RLG00000002317

Methionine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0015103
Physical Location & Seq
Reverse (-)
32135409 .. 32141967
6559 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002317

Sequence Viewer

Length: 3297 bp
ATGGCGAAGAACGACGCGTCGTCGTTGGCGTTGGGATCGGTGGACGAGTTCCTGAAGCGGTGCGAGCAATCCGGCGATGCCGCGTACTCTGCTCTGAGATCAGTGCTGGAGCGTCTCGAGGATCCGAAAATCCGAACTCAAGCTCGGATCTTCCTCTCCAACCTCCAGAAGCGCTTCCCTACCAAGGACGACTGCGATCACTGCTTCCGTACCTATCATTTCCAGATTGAGGACATCTTCTTCGATCAGTATCAAGGTTACCAGGGTAGGAAGAAATTGACAATGATGGTTATCCCTAGCATTTTTGTTCCAGAAGATTGGTCCTTCACTTTTTTCGAAGGACTAAATAGACATCCAGACTCTATCTTTAAGGACAAGTCACTTGCTGAGCTTGGCTGTGGAAATGGTTGGATATCCATTGCCATTGCTGAGAAGTGGTTGCCATCCAAGGTGTATGGTCTCGATATCAATCCTAGAGCTGTCAAGATGTCATGGATAAATTTGTATTTAAATGCTTTGGACGAGGAAGGTCAGCCCATCTATGATGCTGAGAAAAAGACCTTGCTGGACAGGGTTGAGTTTCATGAATCAGATCTCCTATCTTATTGCAGAGACAATGATATTCAACTAGAACGAATTGTTGGTTGCATCCCTCAGATTCTTAACCCGAATCCAGATGCAATGTCTAAGATGATAACTGAGAATGCAAGTGAGGAATTTCTACATTCATTGAGCAATTATTGTGCGCTTCAGGGTTTTCTTGAGGATCAATTTGGCTTAGGTCTAATTGCTAGGGCAGTGGAGGAAGGAATAGCTGTCATCAAACCTATGGGGATAATGATCTTCAACATGGGAGGGCGTCCAGGACAAGCTGTTTGTAAACGCTTGTTTGAACGCCGTGGCTTTCATGTTAACAAGCTTTGGCAGACTAAAATTCTTCAGGCTGCAGACACAGATATATCAGCATTAGTTGAAATTGAAAAGAACAGTCCACACCGTTTTGAGTTCTTCATGGGACTTTCTGGAGATCAGCCTATTTGTGCTCGAACAGCATGGGCATATGGAAATGCCGGTGGTCGAATCTCACATGCTTTATCAGTTTACAGCTGTCAACTCCGCCAACCAAATCAGGTCAAGACAATTTTTGAGTTTCTTAAGAATGGCTTCCACGACATTAGCAGTTCTTTAGATTTATCATTTGAAGATGATTCAGTGGCTGATGAGAAGATTCCCTTCCTAGCTTATCTTTCCAGTGTTCTGAAAGGGAGTTCTTTCTGTAAGTATGAACCACCAGCTGGAAGCAAACATTTTCGCAACCTCATTGCTGGGTTTTTGAAAACGTATCACCGTGTTCCACTCACTACTGATAATGTTGTTGTCTTTCCTTCAAGGGCTGTAGCAATAGAAAATGCTCTTCGGTTGTTCTCACCTCGTCTTGCAATTGTTGATGAACATCTGACGCGCCACCTTCCAAGGAACTGGTTAACATCTTTAACAGTTAAGTGTGCAGGAACTGATAATCCTGCAGAGGATTCACTTACAGTTATTGAAGCGCCTCGACAGTCTGATTTGATAATAGAGCTTATAAGGAAGTTGAAGCCACAGGTGGTGGTTACTGGGATAGCTGATTATGAGTCTGTTACTAGTTCAGCCTTTGTACACCTTTTAGATGTTACAAGAGAAGTTGGATCTCGTCTATTCTTAGACATTTCTGATCATTTTGAGCTATCCAGCCTTCCAAGTTCCAATGGAGTCCTCAAATATATAGGGGGTAATGTTCTGCCATCCCATGCAGCAATTATATGTGGCCTAGTGAAAAACAAGGTTTATTCAGATCTCGAAGTAGCTTTTGTAATTTCAGAGGAGGAAAATATTTTCAAGGCCTTGTCAAAGACTGTTGAACTTCTAGAAGGGAGTACAGCACCAATTAGTCAAGGTTATTATGGTTGTCTTTTTCATGAACTTTTAGCTTTTCAACTTGCTGACAGACATCCACCTCCACAGAGGGAATGCACATCAGTTAAATCAGCAGAGATGATTGGTTTTGCTAGTTCAGCAAACTCGGTCCTTAATAATGCTGAGTTGGCAATTAATGAGGCAGGGAACTCTTCCCTGATTCATATGGACGTGGATCAGAGCTTCTTACATGTACCATCACCTGTCAATGCTGCAATTTTTGAAAGCTTTGCCAGACAGAACATAGCTGAGTCAGAAATAGATGTCACCACTAGCATTAAGGAATTCATAAAGAGCAATTATGGTTACCCACTTGATAGCCATACCGAATTTATATATGCTGACAGCTCGCTAGCTCTGTTTAACAAGTTGGTTCTTTGTTGCATACAAGAAGGGGGGACATTGTGCTTTCCTTCTGGCTCAAATGGAAACTATGTCTCTGCTGCAAAATTTTTGAAAGCAAACATTGTAAATATACCTACAAAGATCGAAGAAGGCTTTAAACTGACAGAAAAGGGTCTTTCTGGAGTCCTAGAGACTCTGGATAAACCATGGGTATACATTTCTGGACCAACAATTAACCCAACTGGTGCGCTATACAGCAACAAAGAGATTGAAAATTTACTGTCTACCTGTGCCAAATTTGGGGCTAGGGTTGTGATTGACACTTCATTCTCGGGTTTGGAATTTGACCCTGAGGGTTGGGGTGGCTGGAGTCTGGTGGACAGTTTGTCAAAGTTATATTCTTCTAGCGAACCATCTTTTTGTGTCTCTCTGCTCGGGGGATTGTCTTTGAAGATGCTGAGTGGGGGACTCAAATTTGGGTTTCTGGTTCTGAACCAGTCTGCTGTGGTGGAAACATTTTATAGCTTTCCAGGATTAAGCAAACCTCATAACACTGTTAAATATGCCGTTAAGAAGCTACTGGGTCTGAGAGAGCAGAAATCAGGAGATCTTTGGGATGCCATTGCAGAACAGATAGGAAATTTGAAGAGTCGATCCAAGCGCTTGAAGGAGACACTGGAGAAGTGTGGATGGGATGTGCTTGAATCTAGTGGTGGTGTTTCCATGGTGGCCAAGCCCTCATCTTATTTGAATAAGACTGTTAAGTTCAAGCAATATAAAGATGGAGGCAGCACTGAAGATGGAACAGTGCATGAAGTTAAGCTTGATGACTCTAATATCAGGGAGGTCATTCACAAATCTACTGGCTTATGCATCAATAGTGGCTCCTGGACTGGAATTCCTGGCTATTGTCGTTTCACCATTGCTCTTGAAGAAAGTGAGTTTGAGGGGGCATTGGATTGCATTGTTCAATTTAAGAAGATCATCAGTAACTGA

Protein Analysis

1099

Amino Acids

121.71

Weight (kDa)

5.62

Isoelectric Point (pI)

39.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 740 - 1087 1.9e-18 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015103)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49810
fragaria_vesca FvH4_5g27550
malus_domestica MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica Prupe.1G586400_v2.0.a1
pyrus_communis pycom08g21570
rosa_chinensis RchiOBHm_Chr7g0218921
rosa_laevigata RLG00000002317
rosa_multiflora Rmu_sc0003823.1_g000008
rosa_roxburghii Rroxscaffold_3G00240310
rosa_rugosa Rorug07G0179800
rosa_samantha Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana Rw7G027380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1586
AccB7I CCANNNNNTGG 1 cut(s) 1295
AccI GTMKAC 2 cut(s) 2514, 2585
AccII CGCG 3 cut(s) 17, 83, 1462
AciI CCGC 3 cut(s) 58, 81, 1117
AclWI GGATC 8 cut(s) 43, 116, 129, 155, 774, 1696, 2139, 2949
AcoI YGGCCR 1 cut(s) 3030
AcuI CTGAAG 4 cut(s) 74, 734, 923, 3117
AcyI GRCGYC 1 cut(s) 859
AfaI GTAC 5 cut(s) 86, 211, 1659, 1918, 2151
AfeI AGCGCT 2 cut(s) 173, 2963
AfiI CCNNNNNNNGG 4 cut(s) 184, 229, 1295, 2601
AflII CTTAAG 1 cut(s) 1154
AflIII ACRYGT 2 cut(s) 15, 2146
AhdI GACNNNNNGTC 2 cut(s) 19, 2686
AhlI ACTAGT 1 cut(s) 1643
AjiI CACGTC 1 cut(s) 2128
AjnI CCWGG 5 cut(s) 261, 862, 2830, 3188, 3202
AjuI GAANNNNNNNTTGG 2 cut(s) 624, 656
AloI GAACNNNNNNTCC 2 cut(s) 1504, 1536
Alw21I GWGCWC 1 cut(s) 1045
Alw26I GTCTC 7 cut(s) 119, 464, 606, 2398, 2486, 2731, 2966
AlwI GGATC 8 cut(s) 43, 116, 129, 155, 774, 1696, 2139, 2949
AlwNI CAGNNNCTG 3 cut(s) 1217, 1514, 3294
Ama87I CYCGRG 3 cut(s) 116, 2632, 2735
Aor51HI AGCGCT 2 cut(s) 173, 2963
AoxI GGCC 3 cut(s) 1807, 1881, 3030
ApeKI GCWGC 5 cut(s) 944, 1793, 2168, 2399, 3090
ArsI GACNNNNNNTTYG 4 cut(s) 224, 256, 858, 890
AseI ATTAAT 1 cut(s) 2091
Asp700I GAANNNNTTC 2 cut(s) 173, 1163
AspLEI GCGC 6 cut(s) 174, 748, 1464, 1555, 2551, 2964
AspS9I GGNCC 3 cut(s) 321, 2065, 2525
AsuHPI GGTGA 5 cut(s) 1337, 1419, 2148, 2215, 3211
AsuII TTCGAA 1 cut(s) 336
AsuNHI GCTAGC 1 cut(s) 2308
AvaI CYCGRG 3 cut(s) 116, 2632, 2735
AvaII GGWCC 3 cut(s) 321, 2065, 2525
AxyI CCTNAGG 1 cut(s) 2652
BalI TGGCCA 1 cut(s) 3032
BamHI GGATCC 1 cut(s) 121
Bbv12I GWGCWC 1 cut(s) 1045
BbvI GCAGC 5 cut(s) 931, 1805, 2155, 2386, 3102
BccI CCATC 9 cut(s) 280, 451, 545, 1792, 2161, 2722, 2985, 3077, 3095
BceAI ACGGC 2 cut(s) 882, 2852
BciT130I CCWGG 5 cut(s) 263, 864, 2832, 3190, 3204
BclI TGATCA 1 cut(s) 1714
BcoDI GTCTC 7 cut(s) 119, 464, 606, 2398, 2486, 2731, 2966
BcuI ACTAGT 1 cut(s) 1643
BfmI CTRYAG 3 cut(s) 945, 1395, 1524
BfoI RGCGCY 3 cut(s) 175, 1556, 2965
BfrI CTTAAG 1 cut(s) 1154
BglII AGATCT 3 cut(s) 592, 1834, 2908
BisI GCNGC 6 cut(s) 81, 945, 1794, 2169, 2400, 3091
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 6 cut(s) 82, 946, 1795, 2170, 2401, 3092
Bme1390I CCNGG 5 cut(s) 263, 864, 2832, 3190, 3204
Bme18I GGWCC 3 cut(s) 321, 2065, 2525
BmeRI GACNNNNNGTC 2 cut(s) 19, 2686
BmeT110I CYCGRG 3 cut(s) 116, 2632, 2735
BmgBI CACGTC 1 cut(s) 2128
BmgT120I GGNCC 3 cut(s) 321, 2065, 2525
BmiI GGNNCC 2 cut(s) 123, 3187
BmrFI CCNGG 5 cut(s) 263, 864, 2832, 3190, 3204
BmrI ACTGGG 2 cut(s) 1626, 2891
BmsI GCATC 7 cut(s) 67, 535, 657, 667, 2745, 2908, 3183
BmtI GCTAGC 1 cut(s) 2312
BmuI ACTGGG 2 cut(s) 1626, 2891
BpmI CTGGAG 6 cut(s) 128, 149, 1044, 2502, 2689, 2999
Bpu10I CCTNAGC 1 cut(s) 778
Bpu1102I GCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 123, 782
BsaBI GATNNNNATC 5 cut(s) 249, 290, 468, 839, 1452
BsaHI GRCGYC 1 cut(s) 859
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 7 cut(s) 183, 262, 447, 898, 1472, 2507, 3024
Bsc4I CCNNNNNNNGG 4 cut(s) 184, 229, 1295, 2601
Bse118I RCCGGY 1 cut(s) 1070
Bse1I ACTGG 9 cut(s) 1251, 1484, 1621, 2548, 2797, 2886, 2982, 3169, 3199
Bse21I CCTNAGG 1 cut(s) 2652
Bse3DI GCAATG 6 cut(s) 417, 423, 687, 1320, 2922, 3222
Bse8I GATNNNNATC 5 cut(s) 249, 290, 468, 839, 1452
BseBI CCWGG 5 cut(s) 263, 864, 2832, 3190, 3204
BseDI CCNNGG 7 cut(s) 183, 262, 447, 898, 1472, 2507, 3024
BseGI GGATG 8 cut(s) 352, 443, 648, 1784, 1990, 2923, 2996, 3001
BseJI GATNNNNATC 5 cut(s) 249, 290, 468, 839, 1452
BseLI CCNNNNNNNGG 4 cut(s) 184, 229, 1295, 2601
BseMI GCAATG 6 cut(s) 417, 423, 687, 1320, 2922, 3222
BseNI ACTGG 9 cut(s) 1251, 1484, 1621, 2548, 2797, 2886, 2982, 3169, 3199
BseRI GAGGAG 1 cut(s) 1877
BseXI GCAGC 5 cut(s) 931, 1805, 2155, 2386, 3102
BseYI CCCAGC 1 cut(s) 1325
BsgI GTGCAG 1 cut(s) 1527
Bsh1236I CGCG 3 cut(s) 17, 83, 1462
BshFI GGCC 3 cut(s) 1809, 1883, 3032
BsiHKAI GWGCWC 1 cut(s) 1045
BsiHKCI CYCGRG 3 cut(s) 116, 2632, 2735
BsiSI CCGG 2 cut(s) 72, 1071
BslFI GGGAC 3 cut(s) 1029, 2368, 2781
BslI CCNNNNNNNGG 4 cut(s) 184, 229, 1295, 2601
BsmAI GTCTC 7 cut(s) 119, 464, 606, 2398, 2486, 2731, 2966
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 3 cut(s) 1029, 2368, 2781
BsmI GAATGC 2 cut(s) 709, 2015
BsnI GGCC 3 cut(s) 1809, 1883, 3032
Bso31I GGTCTC 1 cut(s) 464
BsoBI CYCGRG 3 cut(s) 116, 2632, 2735
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 1045
Bsp1407I TGTACA 1 cut(s) 1657
Bsp1720I GCTNAGC 1 cut(s) 387
Bsp19I CCATGG 2 cut(s) 2507, 3024
BspACI CCGC 3 cut(s) 58, 81, 1117
BspANI GGCC 3 cut(s) 1809, 1883, 3032
BspFNI CGCG 3 cut(s) 17, 83, 1462
BspHI TCATGA 2 cut(s) 583, 1957
BspLI GGNNCC 2 cut(s) 123, 3187
BspMAI CTGCAG 2 cut(s) 949, 1528
BspOI GCTAGC 1 cut(s) 2312
BspPI GGATC 8 cut(s) 43, 116, 129, 155, 774, 1696, 2139, 2949
BspQI GCTCTTC 1 cut(s) 1419
BspT104I TTCGAA 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 1154
BspTNI GGTCTC 1 cut(s) 464
BsrDI GCAATG 6 cut(s) 417, 423, 687, 1320, 2922, 3222
BsrFI RCCGGY 1 cut(s) 1070
BsrGI TGTACA 1 cut(s) 1657
BsrI ACTGG 9 cut(s) 1251, 1484, 1621, 2548, 2797, 2886, 2982, 3169, 3199
BssAI RCCGGY 1 cut(s) 1070
BssECI CCNNGG 7 cut(s) 183, 262, 447, 898, 1472, 2507, 3024
BssNAI GTATAC 1 cut(s) 2515
BssNI GRCGYC 1 cut(s) 859
BssT1I CCWWGG 5 cut(s) 183, 447, 1472, 2507, 3024
Bst1107I GTATAC 1 cut(s) 2515
Bst2UI CCWGG 5 cut(s) 263, 864, 2832, 3190, 3204
Bst6I CTCTTC 3 cut(s) 1419, 2113, 2942
BstACI GRCGYC 1 cut(s) 859
BstAFI CTTAAG 1 cut(s) 1154
BstAUI TGTACA 1 cut(s) 1657
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 3 cut(s) 65, 2306, 2310
BstDSI CCRYGG 3 cut(s) 898, 2507, 3024
BstEII GGTNACC 2 cut(s) 257, 2261
BstF5I GGATG 8 cut(s) 352, 443, 648, 1784, 1990, 2923, 2996, 3001
BstFNI CGCG 3 cut(s) 17, 83, 1462
BstH2I RGCGCY 3 cut(s) 175, 1556, 2965
BstHHI GCGC 6 cut(s) 174, 748, 1464, 1555, 2551, 2964
BstMAI GTCTC 7 cut(s) 119, 464, 606, 2398, 2486, 2731, 2966
BstMWI GCNNNNNNNGC 6 cut(s) 64, 89, 201, 1049, 2054, 2084
BstNI CCWGG 5 cut(s) 263, 864, 2832, 3190, 3204
BstNSI RCATGY 2 cut(s) 1091, 2150
BstPI GGTNACC 2 cut(s) 257, 2261
BstSCI CCNGG 5 cut(s) 261, 862, 2830, 3188, 3202
BstSFI CTRYAG 3 cut(s) 945, 1395, 1524
BstUI CGCG 3 cut(s) 17, 83, 1462
BstV1I GCAGC 5 cut(s) 931, 1805, 2155, 2386, 3102
BstX2I RGATCY 6 cut(s) 121, 147, 592, 1688, 1834, 2908
BstXI CCANNNNNNTGG 2 cut(s) 318, 1479
BstYI RGATCY 6 cut(s) 121, 147, 592, 1688, 1834, 2908
BstZ17I GTATAC 1 cut(s) 2515
Bsu36I CCTNAGG 1 cut(s) 2652
BsuRI GGCC 3 cut(s) 1809, 1883, 3032
BtgI CCRYGG 3 cut(s) 898, 2507, 3024
BtgZI GCGATG 1 cut(s) 90
BtrI CACGTC 1 cut(s) 2128
BtsCI GGATG 8 cut(s) 352, 443, 648, 1784, 1990, 2923, 2996, 3001
BtsI GCAGTG 2 cut(s) 199, 804
BtsIMutI CAGTG 9 cut(s) 108, 199, 804, 1218, 1258, 2853, 2975, 3093, 3114
Cac8I GCNNGC 3 cut(s) 65, 2306, 2310
CaiI CAGNNNCTG 3 cut(s) 1217, 1514, 3294
CciI TCATGA 2 cut(s) 583, 1957
CfoI GCGC 6 cut(s) 174, 748, 1464, 1555, 2551, 2964
Cfr10I RCCGGY 1 cut(s) 1070
Cfr13I GGNCC 3 cut(s) 321, 2065, 2525
CseI GACGC 5 cut(s) 6, 23, 101, 848, 1468
Csp6I GTAC 5 cut(s) 85, 210, 1658, 1917, 2150
CviQI GTAC 5 cut(s) 85, 210, 1658, 1917, 2150
DraI TTTAAA 2 cut(s) 510, 2458
DriI GACNNNNNGTC 2 cut(s) 19, 2686
EaeI YGGCCR 1 cut(s) 3030
Eam1104I CTCTTC 3 cut(s) 1419, 2113, 2942
Eam1105I GACNNNNNGTC 2 cut(s) 19, 2686
EarI CTCTTC 3 cut(s) 1419, 2113, 2942
EciI GGCGGA 1 cut(s) 1106
Eco130I CCWWGG 5 cut(s) 183, 447, 1472, 2507, 3024
Eco147I AGGCCT 1 cut(s) 1883
Eco31I GGTCTC 1 cut(s) 464
Eco32I GATATC 2 cut(s) 414, 466
Eco47I GGWCC 3 cut(s) 321, 2065, 2525
Eco47III AGCGCT 2 cut(s) 173, 2963
Eco57I CTGAAG 4 cut(s) 74, 734, 923, 3117
Eco81I CCTNAGG 1 cut(s) 2652
Eco88I CYCGRG 3 cut(s) 116, 2632, 2735
Eco91I GGTNACC 2 cut(s) 257, 2261
EcoO65I GGTNACC 2 cut(s) 257, 2261
EcoRI GAATTC 2 cut(s) 2240, 3198
EcoRII CCWGG 5 cut(s) 261, 862, 2830, 3188, 3202
EcoRV GATATC 2 cut(s) 414, 466
EcoT14I CCWWGG 5 cut(s) 183, 447, 1472, 2507, 3024
EcoT22I ATGCAT 1 cut(s) 3176
ErhI CCWWGG 5 cut(s) 183, 447, 1472, 2507, 3024
Esp3I CGTCTC 1 cut(s) 119
FalI AAGNNNNNCTT 6 cut(s) 1148, 1180, 1217, 1249, 3108, 3140
FaqI GGGAC 3 cut(s) 1029, 2368, 2781
FauNDI CATATG 2 cut(s) 1060, 2121
FbaI TGATCA 1 cut(s) 1714
FblI GTMKAC 2 cut(s) 2514, 2585
Fnu4HI GCNGC 6 cut(s) 81, 945, 1794, 2169, 2400, 3091
FokI GGATG 8 cut(s) 339, 430, 635, 1771, 1977, 2930, 3003, 3008
Fsp4HI GCNGC 6 cut(s) 81, 945, 1794, 2169, 2400, 3091
GlaI GCGC 6 cut(s) 173, 747, 1463, 1554, 2550, 2963
GluI GCNGC 6 cut(s) 81, 945, 1794, 2169, 2400, 3091
GsaI CCCAGC 1 cut(s) 1329
GsuI CTGGAG 6 cut(s) 128, 149, 1044, 2502, 2689, 2999
HaeII RGCGCY 3 cut(s) 175, 1556, 2965
HaeIII GGCC 3 cut(s) 1809, 1883, 3032
HapII CCGG 2 cut(s) 72, 1071
HgaI GACGC 5 cut(s) 6, 23, 101, 848, 1468
HhaI GCGC 6 cut(s) 174, 748, 1464, 1555, 2551, 2964
Hin1I GRCGYC 1 cut(s) 859
Hin6I GCGC 6 cut(s) 172, 746, 1462, 1553, 2549, 2962
HinP1I GCGC 6 cut(s) 172, 746, 1462, 1553, 2549, 2962
HincII GTYRAC 3 cut(s) 913, 1112, 1485
HindII GTYRAC 3 cut(s) 913, 1112, 1485
HindIII AAGCTT 3 cut(s) 917, 2182, 3122
HpaI GTTAAC 2 cut(s) 913, 1485
HpaII CCGG 2 cut(s) 72, 1071
HphI GGTGA 5 cut(s) 1337, 1419, 2148, 2215, 3211
Hpy99I CGWCG 3 cut(s) 17, 22, 25
HpyCH4IV ACGT 2 cut(s) 1340, 2127
HpyF10VI GCNNNNNNNGC 6 cut(s) 64, 89, 201, 1049, 2054, 2084
HpySE526I ACGT 2 cut(s) 1340, 2127
Hsp92I GRCGYC 1 cut(s) 859
HspAI GCGC 6 cut(s) 172, 746, 1462, 1553, 2549, 2962
Ksp22I TGATCA 1 cut(s) 1714
KspAI GTTAAC 2 cut(s) 913, 1485
LguI GCTCTTC 1 cut(s) 1419
LmnI GCTCC 2 cut(s) 109, 3191
Lsp1109I GCAGC 5 cut(s) 931, 1805, 2155, 2386, 3102
LweI GCATC 7 cut(s) 67, 535, 657, 667, 2745, 2908, 3183
MaeII ACGT 2 cut(s) 1340, 2127
MaeIII GTNAC 8 cut(s) 257, 378, 1612, 1639, 1672, 2221, 2261, 3290
MfeI CAATTG 1 cut(s) 1440
MflI RGATCY 6 cut(s) 121, 147, 592, 1688, 1834, 2908
MhlI GDGCHC 1 cut(s) 1045
MlsI TGGCCA 1 cut(s) 3032
MluI ACGCGT 1 cut(s) 15
MluNI TGGCCA 1 cut(s) 3032
MmeI TCCRAC 3 cut(s) 183, 389, 1666
Mox20I TGGCCA 1 cut(s) 3032
Mph1103I ATGCAT 1 cut(s) 3176
MroXI GAANNNNTTC 2 cut(s) 173, 1163
MscI TGGCCA 1 cut(s) 3032
Msp20I TGGCCA 1 cut(s) 3032
MspA1I CMGCKG 2 cut(s) 1107, 1295
MspCI CTTAAG 1 cut(s) 1154
MspI CCGG 2 cut(s) 72, 1071
MspR9I CCNGG 5 cut(s) 263, 864, 2832, 3190, 3204
MunI CAATTG 1 cut(s) 1440
Mva1269I GAATGC 2 cut(s) 709, 2015
MvaI CCWGG 5 cut(s) 263, 864, 2832, 3190, 3204
MvnI CGCG 3 cut(s) 17, 83, 1462
MwoI GCNNNNNNNGC 6 cut(s) 64, 89, 201, 1049, 2054, 2084
NcoI CCATGG 2 cut(s) 2507, 3024
NdeI CATATG 2 cut(s) 1060, 2121
NheI GCTAGC 1 cut(s) 2308
NlaIV GGNNCC 2 cut(s) 123, 3187
NmuCI GTSAC 2 cut(s) 378, 2221
NsiI ATGCAT 1 cut(s) 3176
NspI RCATGY 2 cut(s) 1091, 2150
NspV TTCGAA 1 cut(s) 336
PaeR7I CTCGAG 1 cut(s) 116
PagI TCATGA 2 cut(s) 583, 1957
PceI AGGCCT 1 cut(s) 1883
PciI ACATGT 1 cut(s) 2146
PciSI GCTCTTC 1 cut(s) 1419
PcsI WCGNNNNNNNCGW 1 cut(s) 26
PctI GAATGC 2 cut(s) 709, 2015
PdmI GAANNNNTTC 2 cut(s) 173, 1163
PfeI GAWTC 9 cut(s) 587, 658, 670, 1080, 1208, 1228, 1532, 2116, 3005
PflMI CCANNNNNTGG 1 cut(s) 1295
PfoI TCCNGGA 3 cut(s) 862, 2830, 3188
PkrI GCNGC 6 cut(s) 82, 946, 1795, 2170, 2401, 3092
PscI ACATGT 1 cut(s) 2146
PshBI ATTAAT 1 cut(s) 2091
PsiI TTATAA 1 cut(s) 1586
Psp6I CCWGG 5 cut(s) 261, 862, 2830, 3188, 3202
PspEI GGTNACC 2 cut(s) 257, 2261
PspFI CCCAGC 1 cut(s) 1325
PspGI CCWGG 5 cut(s) 261, 862, 2830, 3188, 3202
PspN4I GGNNCC 2 cut(s) 123, 3187
PspPI GGNCC 3 cut(s) 321, 2065, 2525
PstI CTGCAG 2 cut(s) 949, 1528
PstNI CAGNNNCTG 3 cut(s) 1217, 1514, 3294
PsuI RGATCY 6 cut(s) 121, 147, 592, 1688, 1834, 2908
PvuII CAGCTG 2 cut(s) 1107, 1295
RsaI GTAC 5 cut(s) 86, 211, 1659, 1918, 2151
RsaNI GTAC 5 cut(s) 85, 210, 1658, 1917, 2150
SapI GCTCTTC 1 cut(s) 1419
SatI GCNGC 6 cut(s) 81, 945, 1794, 2169, 2400, 3091
Sau96I GGNCC 3 cut(s) 321, 2065, 2525
ScrFI CCNGG 5 cut(s) 263, 864, 2832, 3190, 3204
SduI GDGCHC 1 cut(s) 1045
SfaNI GCATC 7 cut(s) 67, 535, 657, 667, 2745, 2908, 3183
SfcI CTRYAG 3 cut(s) 945, 1395, 1524
Sfr274I CTCGAG 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 3 cut(s) 321, 2065, 2525
SlaI CTCGAG 1 cut(s) 116
SmiI ATTTAAAT 1 cut(s) 510
SmlI CTYRAG 4 cut(s) 116, 138, 761, 1154
SmoI CTYRAG 4 cut(s) 116, 138, 761, 1154
SpeI ACTAGT 1 cut(s) 1643
SseBI AGGCCT 1 cut(s) 1883
SsiI CCGC 3 cut(s) 58, 81, 1117
SspI AATATT 1 cut(s) 1873
StuI AGGCCT 1 cut(s) 1883
StyD4I CCNGG 5 cut(s) 261, 862, 2830, 3188, 3202
StyI CCWWGG 5 cut(s) 183, 447, 1472, 2507, 3024
SwaI ATTTAAAT 1 cut(s) 510
TaiI ACGT 2 cut(s) 1343, 2130
TaqII GACCGA 1 cut(s) 2053
TatI WGTACW 2 cut(s) 1657, 1916
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 9 cut(s) 587, 658, 670, 1080, 1208, 1228, 1532, 2116, 3005
TscAI CASTG 9 cut(s) 108, 206, 804, 1218, 1258, 2860, 2982, 3100, 3114
TseFI GTSAC 2 cut(s) 378, 2221
TseI GCWGC 5 cut(s) 944, 1793, 2168, 2399, 3090
Tsp45I GTSAC 2 cut(s) 378, 2221
TspGWI ACGGA 1 cut(s) 197
TspRI CASTG 9 cut(s) 108, 206, 804, 1218, 1258, 2860, 2982, 3100, 3114
Van91I CCANNNNNTGG 1 cut(s) 1295
Vha464I CTTAAG 1 cut(s) 1154
VpaK11BI GGWCC 3 cut(s) 321, 2065, 2525
VspI ATTAAT 1 cut(s) 2091
XbaI TCTAGA 1 cut(s) 1906
XceI RCATGY 2 cut(s) 1091, 2150
XhoI CTCGAG 1 cut(s) 116
XmiI GTMKAC 2 cut(s) 2514, 2585
XmnI GAANNNNTTC 2 cut(s) 173, 1163
Zsp2I ATGCAT 1 cut(s) 3176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.