Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr08

Orthogroup ID

OG0015103

Physical Location & Seq

Genomic Coordinates Reverse (-)

31352025 .. 31358989

Gene Structure 6965 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD08G1249200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3228 bp

ATGGCGAAGAACGAGGCGCCGTTGGGATCGGTGGACGACTTCTTGAAGCAATGTAAGCAGTCGGGCGATGCCGCGTACGCTGCCTTAAGATCAGTGCTGGAGCGTCTCGAGGATCCGAAAACCAGAACTCAAGCTCGGATCTTCCTCACGGACCTCCAGAACCGCTTCCCCTCCAAGGAGGCCTGCGATCAATGCTTTCGCACATACCATTTTCAGATCGAAGATATCTTCTTCGATCAGTATGAAGGTTACCAGGGTAGGAAGAAATTGACGATGATGGTTATCCCCAGTATATTTGTTCCAGAAGACTGGTCTTTTACTTTTTTTGAAGGACTAAATAGACATTCCGATTCTATCTTCAAGGACAAGTCAGTTGCTGAGCTTGGTTGTGGAAATGGTTGGATATCCATTGCCATTGCTGAGAAGTGGTTGCCATTGAAGGTGTATGGCCTTGAAATCAATCCTAGAGCAGTAAAGATGTCATGGATAAACTTGTACTTAAATGCGTTGGATGAAAGAGGTCAACCCATTTATGATGCGGAGAAGAAAACTTTGCTGGACAGGGTAGAGTTTCATGAATCGGATCTACTATCTTATTGTAGAGATAATGACATTCAACTAGAACGAATTGTTGGATGCATACCTCAGATTCTTAACCCAAATCCAGATGCTATGTCTAAAATGATTACAGAGAACGCAAGTGAGGAATTTCTACATTCATTGAGCAATTATTGTGCACTTCAGGGTTTTCTTGAGGATCAGTTTGGCCTAGGCCTAATTGCTCGGGCAGTGGAGGAAGGAATAGCTGTCATTAAACCTATGGGAATAATGATCTTCAACATGGGCGGTCGTCCAGGACAAGCTGTTTGTAAGCGCTTGTTTGAACGCCGTGGTTTCCATGTTAACAAGCTTTGGCAAACCAAAATTCTCCAGGCTGCAGACACAGATATCTCAGCCTTAGTTGAAATTGAAAAGAACAGTCCACACCGTTTTGAGTTCTTTATGGGGCTTTCTGGAGATCAGCCTATTTGTGCTCGTACAGCCTGGGCTTATGGAAATGCTGGTGGGCGAATCTCTCATGCTTTATCAGTATACAGCTGTCAACTTCGTCAACCAAATCAGGTGAAGACAATTTTTGAGTTTCTTGAAAATGGCTTCCACGAGATAAGCAGTTCTTTAGACTTATCATTTGAAGATGATGCTGTGGCTGATGAGAAGATTCCTTTCCTAGCTTATCTATCCAGTGTTCTGAAAGGGAGTTCTTTTGGCACTTATGAGCCACCAGCTGGAAGCAAACATTTTCGTAGTCTCATTGCCGGGTTTATGAGAACATATCACCGCATTCCACTCAAAGCTGATAATGTGGTTGTCTTTCCTTCAAGGGCTGTGGCGATAGAAAATGCTCTTCGACTGTTCTCGCCTCGTCTTGCAATTGTTGATGAACATCTGACCCGACACCTTCCAAGGGAGTGGTTAACATCGTTAGCAATTGAGTGTGCTGGAACTGATAATCCTTCAGAAGATGTACTTACAGTTATTCAAGCGCCTCGCCAGTCTGACTTAATGATAGAGCTAATAAAGAAGCTGAAGCCACAGGTGGTGGTTACTGGTATTGCGGATTATGAGGCTGTTACTAGTTCAGCTTTTGTGCACCTTTTAGATGTCACAAGAGAAATTGGATCTCGTTTGTTCTTGGACATATCTGATCACTTTGAGCTATCCAGCCTTCCAGGTTCCAATGGAGTCCTCAAATATATTGGAGGAACTGCTCTGCCATCACATGCAGCAATTATATGCGGCTTAGTGAAAAATAAGGTTTATTCGGATCTAGAAGTAGCTTTTGTAATTTCTGAAGAGGAGGCCATCTTCAAGGCATTGTCGAAGACTGTTGAACTACTAGAAGGGAATACGGCACCAATTAGTCAATTTTATTATGGTTGTCTTTTTCATGAACTTTTGGCTTTTCAGCTCGCTGACAGACATCCTCCTGCACAGAGGGAATCTGCATTGCCTAAATCAGCAGAGATGATCGGGTTTGCTAGTTCAGCAATCTCAGTCCTCAATAATGCAGAGTTGTCAATTAGCGAGGCAGAGAACTCTTCCCTCATTCACATGGATGTTGATCAAAGCTTCTTGCGTGTACCGTCACCTGTCAAGGCTGCTATTTTTGAAAGTTTTGCTAGACAGAATATAGCTGAGTCAGAAATAGATGTCACCACTAGCATCAAGCAGTTTATTAAGAGTACTTACGGTTACCCAGTTGACAGCAGCACCGAATTTATATATGCTGACAGCTCGCTAGCTCTGTTCAACAAGATGGTTCTTTGCTGCATTCAGGAAGGGGGTACATTGTGTTTTCCTGCTGGTGCAAATGGGAACTATGTTTCTGCTGCCAAATTTTTGAAAGCAAATATTGTAACTATACCTACTAACCCCACAGATGGCTTTAAACTGACAGACAAGGTGCTTTCTGGAGCGCTAGGGACTGTGAATAAGCCATGGGTGTACATTTCTGGGCCAACAATCAACCCAACTGGTTTGATATACAGCAACAAAGAGATTGAAAGTTTGCTGTCTGCATGTGCTAAAGTTGGGGCTAGAGTTGTGATTGATACTTCCTTCTCAGGCTTGGAATATGACATCGAGGGTTGGGGAGGCTGGAACCTGGTGGACAGTTTGTCAAAGCTAAATACTAGCAACACATGTTTTTGTGTCTCTCTGCTTGGGGGATTATCTTTAAAGATGTTGAGTGGGGCGCTCAAATTCGGGTTCCTTGTATTAAACCAGCCTGTTTTGGTGGATACATTTGATAGCTTTCCAGGATTGAGCAAACCTCACAACACTGTTAAATATGCTGTTAAGAAGCTGCTTAGTCTAAGAGAGAAGAAACCAGGAGGTCTGTGGGATGCTATTGCAGAACATATAAAAACTTTGAAGAGTCAATCCAAGCGCCTGAAAGAGACACTGGAGAAGTGTGGGTGGGATGTGGTTGAACCGTGTGGTGGTGTTTCCATGGTGGCCAAGCCGACGTCCTATCTGAACAAGAGTGTTAAGGTTGATGACTCGAACATCAGGGAAGTCATTCACAAGGCTACAGGGTTATGCATCAACAGTGGAGCCTGGACTGGAATTCCTGGCTACTGTCGATTCACCATTGCTCATGAAGAAAGTGAATTTGAGCGCGCTTTGGATTGCATTGTTAAATTTAAGGACACAATCAACAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001887 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006732 GO:0006790 GO:0008150 GO:0008152 GO:0008168 GO:0008757 GO:0009987 GO:0016740 GO:0016741 GO:0017144 GO:0032259 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0046500 GO:0051186 GO:0071704

KEGG Pathways

Metabolic & Signaling

ko00450 map00450

Pfam Domains

Protein Families

Aminotran_1_2 MTS Methyltransf_31

Protein Analysis

1076

Amino Acids

119.02

Weight (kDa)

5.72

Isoelectric Point (pI)

36.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Aminotran_1_2	PF00155	737 - 1065	7.2e-17	Aminotransferase class I and II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0015103)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G49810
fragaria_vesca	FvH4_5g27550
malus_domestica	MD08G1249200.v1.1 MD15G1443800.v1.1
prunus_persica	Prupe.1G586400_v2.0.a1
pyrus_communis	pycom08g21570
rosa_chinensis	RchiOBHm_Chr7g0218921
rosa_laevigata	RLG00000002317
rosa_multiflora	Rmu_sc0003823.1_g000008
rosa_roxburghii	Rroxscaffold_3G00240310
rosa_rugosa	Rorug07G0179800
rosa_samantha	Rh7AG323100 Rh7BG314100 Rh7CG339800 Rh7DG320200
rosa_wichuraiana	Rw7G027380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	3032
AccB1I	GGYRCC	2 cut(s)	16, 1912
AccB7I	CCANNNNNTGG	1 cut(s)	1286
AccI	GTMKAC	1 cut(s)	1092
AccII	CGCG	2 cut(s)	74, 3183
AciI	CCGC	7 cut(s)	72, 163, 539, 846, 1339, 1616, 1797
AclWI	GGATC	8 cut(s)	34, 107, 120, 146, 591, 765, 1687, 1833
AcoI	YGGCCR	1 cut(s)	3018
AcsI	RAATTY	8 cut(s)	707, 924, 2276, 2396, 2762, 3129, 3173, 3203
AcuI	CTGAAG	4 cut(s)	725, 1500, 1607, 1872
AcyI	GRCGYC	2 cut(s)	17, 3029
AfaI	GTAC	8 cut(s)	77, 497, 1039, 1527, 2142, 2245, 2347, 2507
AfeI	AGCGCT	2 cut(s)	875, 2478
AfiI	CCNNNNNNNGG	5 cut(s)	175, 1286, 1465, 2441, 3002
AflII	CTTAAG	1 cut(s)	85
AflIII	ACRYGT	1 cut(s)	2702
AhdI	GACNNNNNGTC	1 cut(s)	2677
AhlI	ACTAGT	1 cut(s)	1634
AjuI	GAANNNNNNNTTGG	2 cut(s)	615, 647
AloI	GAACNNNNNNTCC	2 cut(s)	1495, 1527
Alw21I	GWGCWC	3 cut(s)	739, 1036, 1653
Alw26I	GTCTC	4 cut(s)	110, 1313, 2719, 2954
Alw44I	GTGCAC	2 cut(s)	735, 1649
AlwI	GGATC	8 cut(s)	34, 107, 120, 146, 591, 765, 1687, 1833
AlwNI	CAGNNNCTG	1 cut(s)	377
Ama87I	CYCGRG	2 cut(s)	107, 783
Aor51HI	AGCGCT	2 cut(s)	875, 2478
AoxI	GGCC	7 cut(s)	180, 448, 766, 772, 1860, 2516, 3018
ApaLI	GTGCAC	2 cut(s)	735, 1649
ApeKI	GCWGC	8 cut(s)	80, 935, 1784, 2159, 2268, 2328, 2390, 2866
ApoI	RAATTY	8 cut(s)	707, 924, 2276, 2396, 2762, 3129, 3173, 3203
ArsI	GACNNNNNNTTYG	4 cut(s)	849, 881, 1172, 1204
Asp700I	GAANNNNTTC	2 cut(s)	164, 3081
AspA2I	CCTAGG	1 cut(s)	769
AspLEI	GCGC	8 cut(s)	19, 876, 1546, 2479, 2758, 2952, 3183, 3185
AspS9I	GGNCC	2 cut(s)	151, 2516
AsuC2I	CCSGG	1 cut(s)	1318
AsuHPI	GGTGA	5 cut(s)	1135, 1328, 2139, 2206, 3142
AsuNHI	GCTAGC	1 cut(s)	2299
AvaI	CYCGRG	2 cut(s)	107, 783
AvaII	GGWCC	1 cut(s)	151
AvrII	CCTAGG	1 cut(s)	769
BaeGI	GKGCMC	2 cut(s)	739, 1653
BalI	TGGCCA	1 cut(s)	3020
BamHI	GGATCC	1 cut(s)	112
BanI	GGYRCC	2 cut(s)	16, 1912
BauI	CACGAG	1 cut(s)	1160
BbsI	GAAGAC	3 cut(s)	312, 1133, 1889
Bbv12I	GWGCWC	3 cut(s)	739, 1036, 1653
BbvI	GCAGC	8 cut(s)	67, 922, 1796, 2146, 2280, 2315, 2377, 2853
BccI	CCATC	5 cut(s)	271, 1783, 1871, 2311, 2435
BceAI	ACGGC	3 cut(s)	4, 873, 1926
BciVI	GTATCC	1 cut(s)	2794
BclI	TGATCA	2 cut(s)	1705, 2122
BcnI	CCSGG	1 cut(s)	1318
BcoDI	GTCTC	4 cut(s)	110, 1313, 2719, 2954
BcuI	ACTAGT	1 cut(s)	1634
BfmI	CTRYAG	2 cut(s)	936, 3093
BfoI	RGCGCY	6 cut(s)	20, 877, 1547, 2480, 2759, 2953
BfrI	CTTAAG	1 cut(s)	85
BfuI	GTATCC	1 cut(s)	2794
BlnI	CCTAGG	1 cut(s)	769
BlpI	GCTNAGC	1 cut(s)	378
BmcAI	AGTACT	1 cut(s)	2245
Bme18I	GGWCC	1 cut(s)	151
BmeRI	GACNNNNNGTC	1 cut(s)	2677
BmeT110I	CYCGRG	2 cut(s)	107, 783
BmgT120I	GGNCC	2 cut(s)	151, 2516
BmiI	GGNNCC	7 cut(s)	18, 114, 1735, 1914, 2663, 2771, 3118
BmrI	ACTGGG	2 cut(s)	282, 2252
BmsI	GCATC	8 cut(s)	58, 526, 626, 658, 1189, 2232, 2896, 3114
BmtI	GCTAGC	1 cut(s)	2303
BmuI	ACTGGG	2 cut(s)	282, 2252
BpiI	GAAGAC	3 cut(s)	312, 1133, 1889
BplI	GAGNNNNNCTC	2 cut(s)	2818, 2850
BpmI	CTGGAG	6 cut(s)	119, 140, 914, 1035, 2493, 2987
Bpu1102I	GCTNAGC	1 cut(s)	378
BpuEI	CTTGAG	2 cut(s)	114, 773
BpuMI	CCSGG	1 cut(s)	1318
BsaBI	GATNNNNATC	3 cut(s)	281, 1443, 2121
BsaHI	GRCGYC	2 cut(s)	17, 3029
BsaJI	CCNNGG	8 cut(s)	174, 253, 769, 889, 1044, 1463, 2498, 3012
Bsc4I	CCNNNNNNNGG	5 cut(s)	175, 1286, 1465, 2441, 3002
Bse1I	ACTGG	9 cut(s)	288, 314, 1242, 1552, 1612, 2258, 2539, 2970, 3130
Bse3DI	GCAATG	6 cut(s)	56, 408, 414, 1311, 2006, 3153
Bse8I	GATNNNNATC	3 cut(s)	281, 1443, 2121
BseDI	CCNNGG	8 cut(s)	174, 253, 769, 889, 1044, 1463, 2498, 3012
BseGI	GGATG	6 cut(s)	517, 641, 1981, 2122, 2911, 2989
BseJI	GATNNNNATC	3 cut(s)	281, 1443, 2121
BseLI	CCNNNNNNNGG	5 cut(s)	175, 1286, 1465, 2441, 3002
BseMI	GCAATG	6 cut(s)	56, 408, 414, 1311, 2006, 3153
BseMII	CTCAG	7 cut(s)	369, 411, 659, 966, 2067, 2187, 2637
BseNI	ACTGG	9 cut(s)	288, 314, 1242, 1552, 1612, 2258, 2539, 2970, 3130
BsePI	GCGCGC	1 cut(s)	3181
BseRI	GAGGAG	1 cut(s)	1871
BseSI	GKGCMC	2 cut(s)	739, 1653
BseXI	GCAGC	8 cut(s)	67, 922, 1796, 2146, 2280, 2315, 2377, 2853
BsgI	GTGCAG	1 cut(s)	1974
Bsh1236I	CGCG	2 cut(s)	74, 3183
Bsh1285I	CGRYCG	1 cut(s)	850
BshFI	GGCC	7 cut(s)	182, 450, 768, 774, 1862, 2518, 3020
BshNI	GGYRCC	2 cut(s)	16, 1912
BsiEI	CGRYCG	1 cut(s)	850
BsiHKAI	GWGCWC	3 cut(s)	739, 1036, 1653
BsiHKCI	CYCGRG	2 cut(s)	107, 783
BsiSI	CCGG	1 cut(s)	1317
BsiWI	CGTACG	1 cut(s)	75
BslFI	GGGAC	1 cut(s)	2497
BslI	CCNNNNNNNGG	5 cut(s)	175, 1286, 1465, 2441, 3002
BsmAI	GTCTC	4 cut(s)	110, 1313, 2719, 2954
BsmBI	CGTCTC	1 cut(s)	110
BsmFI	GGGAC	1 cut(s)	2497
BsmI	GAATGC	2 cut(s)	1341, 2331
BsnI	GGCC	7 cut(s)	182, 450, 768, 774, 1862, 2518, 3020
BsoBI	CYCGRG	2 cut(s)	107, 783
Bsp1286I	GDGCHC	3 cut(s)	739, 1036, 1653
Bsp1407I	TGTACA	1 cut(s)	2505
Bsp1720I	GCTNAGC	1 cut(s)	378
Bsp19I	CCATGG	2 cut(s)	2498, 3012
BspACI	CCGC	7 cut(s)	72, 163, 539, 846, 1339, 1616, 1797
BspANI	GGCC	7 cut(s)	182, 450, 768, 774, 1862, 2518, 3020
BspCNI	CTCAG	7 cut(s)	370, 412, 658, 965, 2066, 2188, 2636
BspFNI	CGCG	2 cut(s)	74, 3183
BspHI	TCATGA	3 cut(s)	574, 1948, 3160
BspLI	GGNNCC	7 cut(s)	18, 114, 1735, 1914, 2663, 2771, 3118
BspMAI	CTGCAG	1 cut(s)	940
BspOI	GCTAGC	1 cut(s)	2303
BspPI	GGATC	8 cut(s)	34, 107, 120, 146, 591, 765, 1687, 1833
BspQI	GCTCTTC	1 cut(s)	1410
BspT107I	GGYRCC	2 cut(s)	16, 1912
BspTI	CTTAAG	1 cut(s)	85
BsrDI	GCAATG	6 cut(s)	56, 408, 414, 1311, 2006, 3153
BsrGI	TGTACA	1 cut(s)	2505
BsrI	ACTGG	9 cut(s)	288, 314, 1242, 1552, 1612, 2258, 2539, 2970, 3130
BssECI	CCNNGG	8 cut(s)	174, 253, 769, 889, 1044, 1463, 2498, 3012
BssHII	GCGCGC	1 cut(s)	3181
BssNAI	GTATAC	1 cut(s)	1093
BssNI	GRCGYC	2 cut(s)	17, 3029
BssSI	CACGAG	1 cut(s)	1160
BssT1I	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
Bst1107I	GTATAC	1 cut(s)	1093
Bst2BI	CACGAG	1 cut(s)	1160
Bst6I	CTCTTC	4 cut(s)	1410, 1848, 2104, 2930
BstACI	GRCGYC	2 cut(s)	17, 3029
BstAFI	CTTAAG	1 cut(s)	85
BstAUI	TGTACA	1 cut(s)	2505
BstC8I	GCNNGC	5 cut(s)	184, 1971, 2297, 2301, 3183
BstDSI	CCRYGG	3 cut(s)	889, 2498, 3012
BstEII	GGTNACC	2 cut(s)	248, 2252
BstF5I	GGATG	6 cut(s)	517, 641, 1981, 2122, 2911, 2989
BstFNI	CGCG	2 cut(s)	74, 3183
BstH2I	RGCGCY	6 cut(s)	20, 877, 1547, 2480, 2759, 2953
BstHHI	GCGC	8 cut(s)	19, 876, 1546, 2479, 2758, 2952, 3183, 3185
BstMAI	GTCTC	4 cut(s)	110, 1313, 2719, 2954
BstMCI	CGRYCG	1 cut(s)	850
BstMWI	GCNNNNNNNGC	6 cut(s)	55, 77, 80, 192, 1040, 2045
BstNSI	RCATGY	3 cut(s)	1784, 2583, 2706
BstPI	GGTNACC	2 cut(s)	248, 2252
BstSFI	CTRYAG	2 cut(s)	936, 3093
BstSLI	GKGCMC	2 cut(s)	739, 1653
BstUI	CGCG	2 cut(s)	74, 3183
BstV1I	GCAGC	8 cut(s)	67, 922, 1796, 2146, 2280, 2315, 2377, 2853
BstV2I	GAAGAC	3 cut(s)	312, 1133, 1889
BstX2I	RGATCY	5 cut(s)	112, 138, 583, 1679, 1825
BstXI	CCANNNNNNTGG	2 cut(s)	309, 1470
BstYI	RGATCY	5 cut(s)	112, 138, 583, 1679, 1825
BstZ17I	GTATAC	1 cut(s)	1093
BsuI	GTATCC	1 cut(s)	2794
BsuRI	GGCC	7 cut(s)	182, 450, 768, 774, 1862, 2518, 3020
BtgI	CCRYGG	3 cut(s)	889, 2498, 3012
BtgZI	GCGATG	1 cut(s)	81
BtsCI	GGATG	6 cut(s)	517, 641, 1981, 2122, 2911, 2989
BtsI	GCAGTG	1 cut(s)	795
BtsIMutI	CAGTG	6 cut(s)	99, 795, 1249, 2841, 2963, 3118
Cac8I	GCNNGC	5 cut(s)	184, 1971, 2297, 2301, 3183
CaiI	CAGNNNCTG	1 cut(s)	377
CciI	TCATGA	3 cut(s)	574, 1948, 3160
CfoI	GCGC	8 cut(s)	19, 876, 1546, 2479, 2758, 2952, 3183, 3185
Cfr13I	GGNCC	2 cut(s)	151, 2516
CseI	GACGC	1 cut(s)	92
CsiI	ACCWGGT	1 cut(s)	2664
Csp6I	GTAC	8 cut(s)	76, 496, 1038, 1526, 2141, 2244, 2346, 2506
CspCI	CAANNNNNGTGG	2 cut(s)	1368, 1403
CviQI	GTAC	8 cut(s)	76, 496, 1038, 1526, 2141, 2244, 2346, 2506
DinI	GGCGCC	1 cut(s)	18
DraI	TTTAAA	2 cut(s)	2449, 2739
DriI	GACNNNNNGTC	1 cut(s)	2677
EaeI	YGGCCR	1 cut(s)	3018
Eam1104I	CTCTTC	4 cut(s)	1410, 1848, 2104, 2930
Eam1105I	GACNNNNNGTC	1 cut(s)	2677
EarI	CTCTTC	4 cut(s)	1410, 1848, 2104, 2930
Eco130I	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
Eco147I	AGGCCT	2 cut(s)	182, 774
Eco32I	GATATC	3 cut(s)	226, 405, 949
Eco47I	GGWCC	1 cut(s)	151
Eco47III	AGCGCT	2 cut(s)	875, 2478
Eco57I	CTGAAG	4 cut(s)	725, 1500, 1607, 1872
Eco88I	CYCGRG	2 cut(s)	107, 783
Eco91I	GGTNACC	2 cut(s)	248, 2252
EcoO65I	GGTNACC	2 cut(s)	248, 2252
EcoRI	GAATTC	1 cut(s)	3129
EcoRV	GATATC	3 cut(s)	226, 405, 949
EcoT14I	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
EcoT22I	ATGCAT	2 cut(s)	641, 3107
EgeI	GGCGCC	1 cut(s)	18
EheI	GGCGCC	1 cut(s)	18
ErhI	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
Esp3I	CGTCTC	1 cut(s)	110
FalI	AAGNNNNNCTT	4 cut(s)	1159, 1191, 1512, 1544
FaqI	GGGAC	1 cut(s)	2497
FbaI	TGATCA	2 cut(s)	1705, 2122
FblI	GTMKAC	1 cut(s)	1092
FokI	GGATG	6 cut(s)	524, 648, 1968, 2129, 2918, 2996
GlaI	GCGC	8 cut(s)	18, 875, 1545, 2478, 2757, 2951, 3182, 3184
GsuI	CTGGAG	6 cut(s)	119, 140, 914, 1035, 2493, 2987
HaeII	RGCGCY	6 cut(s)	20, 877, 1547, 2480, 2759, 2953
HaeIII	GGCC	7 cut(s)	182, 450, 768, 774, 1862, 2518, 3020
HapII	CCGG	1 cut(s)	1317
HgaI	GACGC	1 cut(s)	92
HhaI	GCGC	8 cut(s)	19, 876, 1546, 2479, 2758, 2952, 3183, 3185
Hin1I	GRCGYC	2 cut(s)	17, 3029
Hin6I	GCGC	8 cut(s)	17, 874, 1544, 2477, 2756, 2950, 3181, 3183
HinP1I	GCGC	8 cut(s)	17, 874, 1544, 2477, 2756, 2950, 3181, 3183
HincII	GTYRAC	6 cut(s)	524, 904, 1103, 1112, 1476, 2263
HindII	GTYRAC	6 cut(s)	524, 904, 1103, 1112, 1476, 2263
HindIII	AAGCTT	2 cut(s)	908, 2128
HpaI	GTTAAC	2 cut(s)	904, 1476
HpaII	CCGG	1 cut(s)	1317
HphI	GGTGA	5 cut(s)	1135, 1328, 2139, 2206, 3142
Hpy99I	CGWCG	1 cut(s)	3031
HpyCH4IV	ACGT	1 cut(s)	3029
HpyF10VI	GCNNNNNNNGC	6 cut(s)	55, 77, 80, 192, 1040, 2045
HpySE526I	ACGT	1 cut(s)	3029
Hsp92I	GRCGYC	2 cut(s)	17, 3029
HspAI	GCGC	8 cut(s)	17, 874, 1544, 2477, 2756, 2950, 3181, 3183
KasI	GGCGCC	1 cut(s)	16
Ksp22I	TGATCA	2 cut(s)	1705, 2122
KspAI	GTTAAC	2 cut(s)	904, 1476
LguI	GCTCTTC	1 cut(s)	1410
LmnI	GCTCC	3 cut(s)	100, 2474, 3116
Lsp1109I	GCAGC	8 cut(s)	67, 922, 1796, 2146, 2280, 2315, 2377, 2853
LweI	GCATC	8 cut(s)	58, 526, 626, 658, 1189, 2232, 2896, 3114
MabI	ACCWGGT	1 cut(s)	2664
MaeII	ACGT	1 cut(s)	3029
MaeIII	GTNAC	8 cut(s)	248, 1603, 1630, 1663, 2145, 2212, 2252, 2416
MfeI	CAATTG	2 cut(s)	1431, 1488
MflI	RGATCY	5 cut(s)	112, 138, 583, 1679, 1825
MhlI	GDGCHC	3 cut(s)	739, 1036, 1653
MlsI	TGGCCA	1 cut(s)	3020
MluNI	TGGCCA	1 cut(s)	3020
Mly113I	GGCGCC	1 cut(s)	17
MlyI	GAGTC	4 cut(s)	1752, 2207, 2947, 3056
MmeI	TCCRAC	3 cut(s)	380, 489, 613
Mox20I	TGGCCA	1 cut(s)	3020
Mph1103I	ATGCAT	2 cut(s)	641, 3107
MroXI	GAANNNNTTC	2 cut(s)	164, 3081
MscI	TGGCCA	1 cut(s)	3020
MslI	CAYNNNNRTG	2 cut(s)	2111, 2115
Msp20I	TGGCCA	1 cut(s)	3020
MspA1I	CMGCKG	2 cut(s)	1098, 1286
MspCI	CTTAAG	1 cut(s)	85
MspI	CCGG	1 cut(s)	1317
MunI	CAATTG	2 cut(s)	1431, 1488
Mva1269I	GAATGC	2 cut(s)	1341, 2331
MvnI	CGCG	2 cut(s)	74, 3183
MwoI	GCNNNNNNNGC	6 cut(s)	55, 77, 80, 192, 1040, 2045
NarI	GGCGCC	1 cut(s)	17
NciI	CCSGG	1 cut(s)	1318
NcoI	CCATGG	2 cut(s)	2498, 3012
NheI	GCTAGC	1 cut(s)	2299
NlaIV	GGNNCC	7 cut(s)	18, 114, 1735, 1914, 2663, 2771, 3118
NmuCI	GTSAC	3 cut(s)	1663, 2145, 2212
NsiI	ATGCAT	2 cut(s)	641, 3107
NspI	RCATGY	3 cut(s)	1784, 2583, 2706
PaeR7I	CTCGAG	1 cut(s)	107
PagI	TCATGA	3 cut(s)	574, 1948, 3160
PauI	GCGCGC	1 cut(s)	3181
PceI	AGGCCT	2 cut(s)	182, 774
PciI	ACATGT	1 cut(s)	2702
PciSI	GCTCTTC	1 cut(s)	1410
PctI	GAATGC	2 cut(s)	1341, 2331
PdmI	GAANNNNTTC	2 cut(s)	164, 3081
PfeI	GAWTC	7 cut(s)	350, 578, 649, 1071, 1219, 2000, 3147
Pfl23II	CGTACG	1 cut(s)	75
PflMI	CCANNNNNTGG	1 cut(s)	1286
PfoI	TCCNGGA	2 cut(s)	853, 2818
PleI	GAGTC	4 cut(s)	1751, 2206, 2946, 3056
PluTI	GGCGCC	1 cut(s)	20
PpsI	GAGTC	4 cut(s)	1751, 2206, 2946, 3056
PscI	ACATGT	1 cut(s)	2702
PspEI	GGTNACC	2 cut(s)	248, 2252
PspLI	CGTACG	1 cut(s)	75
PspN4I	GGNNCC	7 cut(s)	18, 114, 1735, 1914, 2663, 2771, 3118
PspPI	GGNCC	2 cut(s)	151, 2516
PstI	CTGCAG	1 cut(s)	940
PstNI	CAGNNNCTG	1 cut(s)	377
PsuI	RGATCY	5 cut(s)	112, 138, 583, 1679, 1825
PteI	GCGCGC	1 cut(s)	3181
PvuII	CAGCTG	2 cut(s)	1098, 1286
RsaI	GTAC	8 cut(s)	77, 497, 1039, 1527, 2142, 2245, 2347, 2507
RsaNI	GTAC	8 cut(s)	76, 496, 1038, 1526, 2141, 2244, 2346, 2506
RseI	CAYNNNNRTG	2 cut(s)	2111, 2115
SapI	GCTCTTC	1 cut(s)	1410
Sau96I	GGNCC	2 cut(s)	151, 2516
ScaI	AGTACT	1 cut(s)	2245
SchI	GAGTC	4 cut(s)	1752, 2207, 2947, 3056
SduI	GDGCHC	3 cut(s)	739, 1036, 1653
SexAI	ACCWGGT	1 cut(s)	2664
SfaNI	GCATC	8 cut(s)	58, 526, 626, 658, 1189, 2232, 2896, 3114
SfcI	CTRYAG	2 cut(s)	936, 3093
SfoI	GGCGCC	1 cut(s)	18
Sfr274I	CTCGAG	1 cut(s)	107
SinI	GGWCC	1 cut(s)	151
SlaI	CTCGAG	1 cut(s)	107
SmiMI	CAYNNNNRTG	2 cut(s)	2111, 2115
SmlI	CTYRAG	4 cut(s)	85, 107, 129, 752
SmoI	CTYRAG	4 cut(s)	85, 107, 129, 752
SpeI	ACTAGT	1 cut(s)	1634
SseBI	AGGCCT	2 cut(s)	182, 774
SsiI	CCGC	7 cut(s)	72, 163, 539, 846, 1339, 1616, 1797
SspDI	GGCGCC	1 cut(s)	16
SspI	AATATT	1 cut(s)	2413
StuI	AGGCCT	2 cut(s)	182, 774
StyI	CCWWGG	5 cut(s)	174, 769, 1463, 2498, 3012
TaiI	ACGT	1 cut(s)	3032
TaqI	TCGA	8 cut(s)	108, 219, 234, 1408, 1880, 2643, 3065, 3145
TatI	WGTACW	4 cut(s)	495, 1525, 2243, 2505
TauI	GCSGC	2 cut(s)	74, 1800
TfiI	GAWTC	7 cut(s)	350, 578, 649, 1071, 1219, 2000, 3147
TscAI	CASTG	6 cut(s)	99, 795, 1249, 2848, 2970, 3118
TseFI	GTSAC	3 cut(s)	1663, 2145, 2212
TseI	GCWGC	8 cut(s)	80, 935, 1784, 2159, 2268, 2328, 2390, 2866
Tsp45I	GTSAC	3 cut(s)	1663, 2145, 2212
TspDTI	ATGAA	9 cut(s)	258, 528, 563, 591, 708, 1455, 1937, 1965, 3177
TspGWI	ACGGA	1 cut(s)	164
TspRI	CASTG	6 cut(s)	99, 795, 1249, 2848, 2970, 3118
Van91I	CCANNNNNTGG	1 cut(s)	1286
Vha464I	CTTAAG	1 cut(s)	85
VneI	GTGCAC	2 cut(s)	735, 1649
VpaK11BI	GGWCC	1 cut(s)	151
XapI	RAATTY	8 cut(s)	707, 924, 2276, 2396, 2762, 3129, 3173, 3203
XbaI	TCTAGA	1 cut(s)	1828
XceI	RCATGY	3 cut(s)	1784, 2583, 2706
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2898
XhoI	CTCGAG	1 cut(s)	107
XmaJI	CCTAGG	1 cut(s)	769
XmiI	GTMKAC	1 cut(s)	1092
XmnI	GAANNNNTTC	2 cut(s)	164, 3081
ZraI	GACGTC	1 cut(s)	3030
ZrmI	AGTACT	1 cut(s)	2245
Zsp2I	ATGCAT	2 cut(s)	641, 3107

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.