AT5G54520

WD40 repeats

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008102
Physical Location & Seq
Reverse (-)
22146504 .. 22149302
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54520.1

Sequence Viewer

Length: 1374 bp
ATGGATCTCATCTGCAATTCGTATGCCAATGACTCCGACGACGAACCTGAGCCAGTGACCAATGAGCGATTAACATCTAAAATCGCCGCAGCACCGTCAATTCCTTCTAAGCGTCCATATCCAGTCCCGGAGGAGCGACAATACAAACCACCGCGTAGACCGTATCCTCCACACGGTTCCTATTCGGAATCTCAAACCAGCTCGTCGTTTTCAATACCGGTTCCGGTTCCGGTTCCGGTTCCAGGCCGGTACGTATCCAAGAGAGAGAGATCACTTCTAGCCTCCCTTTCAACGATTCCGACTCCAGATCAAAGTTCCGATTTGAGTCAGAAACCTTATAGCTCTCCTACTGTTCTTGGGAGCATCTCAGATTCTGATGTACCTCGCCATGTTCTATCTTCGGTTAGACATAGACCAAAAGGATCCTCGTTGCAAACCGAAATGCCTAGTAGAATGTCGATTTCGTTAACCGGCCATACAAAGGCTGTTACTGCTATTGATTGGTCTACAAGTCATGTTCATCTTCTTGCTTCTGCGGGGCTTGATGGTGCTGTTTACGTGTGGAATGTGTGGAGTAATGATAAGAAGAAAGTACGGGCTTTTCTCCATCATAATGCACCGGTTAAAGATGTGAAGTGGTCAAAGCAAGGGTTATCGTTGCTTTCTTGTGGATATGACTGCACGTCGAGACTGTTTGATGTTGAAAGAGGGGTAGAGACACAATCTTTCAAGGAGGATGAAGTTGTTGGAGTTGTTAAGTTTCATCCAGATAATTGTAACGTGTTTCTTTCTGGTGGATCAAAGGGAAGTCTTAGATTATGGGACATTAGAGCCAATAAATTCGTGCATGAATATGTTAGAGATCTTGGGCCAATTCTTGATGTTGAGTTTATCGCGGGTGGGAAGCAGTTTATCTCGTCAAGTGATGTTTCTGGCAGAAATATAAGTGAGAATGCTGTTATAGTTTGGGATATTTCGAGAGAGGTTCCTTTATCTAATCAGGTCTATGTAGAAGCATACACATGCCCTTGCATCAAACGTCACCCGCAAGACCCAGTCTTCATCGCGCAATCACACGGGAACTACACAGCAATCTTCTCAACAAACCCACCTTTCAAACTCAACAAATACAAGAGATTCGAAGGTCACTGGGTTGCAGGTTTCCCAATCAAATGCAACTTCAGCCCGGATGGAGAAACCTTAGCCTCTGGCTCATCTGACGGTTCTGTATACATGTACGACTACAAATCCACTGCGCTCATTAAGAAGCTCAAAGCTTATGAACAGCCCTGCGTTAATGTGTCATACCACCCCGTTTTACCTAACGTGGTCGCGGCTTGTAGCTGGAATGGACAAGTCTCTGTTTTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

457

Amino Acids

50.61

Weight (kDa)

8.24

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 145 - 240 4.5e-10 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 151 - 241 3.3e-11 WDR3 first beta-propeller domain
Beta-prop_RIG_2nd PF23775 151 - 201 1e-05 RIG second beta-propeller
Beta-prop_THOC3 PF25174 152 - 194 6.8e-06 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 152 - 287 9e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 152 - 244 9.1e-06 TEP-1 second beta-propeller
WD40_Prp19 PF24814 153 - 309 4.7e-19 Prp19 WD40 domain
Beta-prop_Aladin PF25460 154 - 234 9.8e-06 Aladin seven-bladed propeller
EIF3I PF24805 154 - 280 2.5e-08 EIF3I
WD40 PF00400 155 - 189 5.5e-06 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 156 - 455 2.8e-36 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 159 - 326 2.3e-12 CDC20/Fizzy WD40 domain
WDR55 PF24796 163 - 308 1.6e-15 WDR55
WD40_RFWD3 PF23419 194 - 279 4e-06 E3 ubiquitin-protein ligase RFWD3 WD40 domain
Beta-prop_THOC3 PF25174 204 - 437 3.3e-24 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 318 - 451 1.5e-06 WDR3 first beta-propeller domain
WD40_WDHD1_1st PF24817 319 - 454 2.8e-07 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 347 - 457 5.8e-06 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1148
AccI GTMKAC 3 cut(s) 157, 506, 1230
AccII CGCG 4 cut(s) 154, 896, 1067, 1334
AciI CCGC 6 cut(s) 87, 152, 536, 896, 1046, 1334
AclWI GGATC 4 cut(s) 12, 417, 430, 805
AcoI YGGCCR 1 cut(s) 472
AcsI RAATTY 1 cut(s) 839
AcuI CTGAAG 1 cut(s) 1165
AfaI GTAC 4 cut(s) 251, 381, 594, 1238
AfiI CCNNNNNNNGG 3 cut(s) 173, 242, 481
AflIII ACRYGT 3 cut(s) 558, 780, 1233
AgeI ACCGGT 2 cut(s) 217, 619
AgsI TTSAA 5 cut(s) 213, 291, 704, 730, 1117
AhdI GACNNNNNGTC 1 cut(s) 682
AjiI CACGTC 1 cut(s) 684
AjnI CCWGG 1 cut(s) 241
AluBI AGCT 5 cut(s) 201, 342, 1270, 1277, 1344
AluI AGCT 5 cut(s) 201, 342, 1270, 1277, 1344
Alw26I GTCTC 3 cut(s) 682, 710, 1363
AlwI GGATC 4 cut(s) 12, 417, 430, 805
AlwNI CAGNNNCTG 1 cut(s) 374
AoxI GGCC 3 cut(s) 244, 472, 869
ApeKI GCWGC 1 cut(s) 89
ApoI RAATTY 1 cut(s) 839
AsiGI ACCGGT 2 cut(s) 217, 619
AspLEI GCGC 2 cut(s) 1069, 1258
AspS9I GGNCC 1 cut(s) 869
AsuC2I CCSGG 2 cut(s) 128, 1187
AsuHPI GGTGA 1 cut(s) 1034
AsuII TTCGAA 1 cut(s) 1140
BamHI GGATCC 1 cut(s) 422
BbsI GAAGAC 1 cut(s) 1051
BbvI GCAGC 1 cut(s) 101
BccI CCATC 3 cut(s) 539, 615, 1184
BciT130I CCWGG 1 cut(s) 243
BciVI GTATCC 2 cut(s) 174, 265
BcnI CCSGG 2 cut(s) 128, 1187
BcoDI GTCTC 3 cut(s) 682, 710, 1363
BfaI CTAG 2 cut(s) 278, 447
BfuAI ACCTGC 1 cut(s) 1148
BfuI GTATCC 2 cut(s) 174, 265
BglII AGATCT 1 cut(s) 862
BisI GCNGC 3 cut(s) 87, 90, 1335
BlsI GCNGC 3 cut(s) 88, 91, 1336
Bme1390I CCNGG 3 cut(s) 128, 243, 1187
BmeRI GACNNNNNGTC 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 684
BmgT120I GGNCC 1 cut(s) 869
BmiI GGNNCC 7 cut(s) 178, 222, 228, 234, 240, 424, 987
BmrFI CCNGG 3 cut(s) 128, 243, 1187
BmrI ACTGGG 2 cut(s) 1049, 1159
BmsI GCATC 2 cut(s) 372, 1041
BmuI ACTGGG 2 cut(s) 1049, 1159
BpiI GAAGAC 1 cut(s) 1051
BpmI CTGGAG 1 cut(s) 288
Bpu10I CCTNAGC 2 cut(s) 48, 1201
Bpu14I TTCGAA 1 cut(s) 1140
BpuMI CCSGG 2 cut(s) 128, 1187
BsaAI YACGTR 2 cut(s) 253, 559
BsaBI GATNNNNATC 1 cut(s) 73
BsaWI WCCGGW 5 cut(s) 217, 223, 229, 235, 619
Bsc4I CCNNNNNNNGG 3 cut(s) 173, 242, 481
Bse118I RCCGGY 4 cut(s) 217, 246, 470, 619
Bse1I ACTGG 4 cut(s) 53, 122, 1055, 1154
Bse8I GATNNNNATC 1 cut(s) 73
BseBI CCWGG 1 cut(s) 243
BseGI GGATG 3 cut(s) 742, 763, 1195
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 3 cut(s) 173, 242, 481
BseMII CTCAG 2 cut(s) 39, 381
BseNI ACTGG 4 cut(s) 53, 122, 1055, 1154
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 1 cut(s) 101
BsgI GTGCAG 1 cut(s) 664
Bsh1236I CGCG 4 cut(s) 154, 896, 1067, 1334
BshFI GGCC 3 cut(s) 246, 474, 871
BshTI ACCGGT 2 cut(s) 217, 619
BsiSI CCGG 9 cut(s) 128, 218, 224, 230, 236, 247, 471, 620, 1187
BslFI GGGAC 2 cut(s) 110, 836
BslI CCNNNNNNNGG 3 cut(s) 173, 242, 481
BsmAI GTCTC 3 cut(s) 682, 710, 1363
BsmFI GGGAC 2 cut(s) 110, 836
BsmI GAATGC 1 cut(s) 958
BsnI GGCC 3 cut(s) 246, 474, 871
Bsp119I TTCGAA 1 cut(s) 1140
Bsp143I GATC 6 cut(s) 4, 269, 307, 422, 797, 862
BspACI CCGC 6 cut(s) 87, 152, 536, 896, 1046, 1334
BspANI GGCC 3 cut(s) 246, 474, 871
BspCNI CTCAG 2 cut(s) 40, 380
BspFNI CGCG 4 cut(s) 154, 896, 1067, 1334
BspLI GGNNCC 7 cut(s) 178, 222, 228, 234, 240, 424, 987
BspMI ACCTGC 1 cut(s) 1148
BspPI GGATC 4 cut(s) 12, 417, 430, 805
BspT104I TTCGAA 1 cut(s) 1140
BsrFI RCCGGY 4 cut(s) 217, 246, 470, 619
BsrI ACTGG 4 cut(s) 53, 122, 1055, 1154
BssAI RCCGGY 4 cut(s) 217, 246, 470, 619
BssMI GATC 6 cut(s) 4, 269, 307, 422, 797, 862
BssNAI GTATAC 1 cut(s) 1231
Bst1107I GTATAC 1 cut(s) 1231
Bst2UI CCWGG 1 cut(s) 243
Bst4CI ACNGT 6 cut(s) 96, 162, 176, 352, 693, 1223
BstBAI YACGTR 2 cut(s) 253, 559
BstBI TTCGAA 1 cut(s) 1140
BstDEI CTNAG 5 cut(s) 48, 108, 367, 812, 1201
BstF5I GGATG 3 cut(s) 742, 763, 1195
BstFNI CGCG 4 cut(s) 154, 896, 1067, 1334
BstHHI GCGC 2 cut(s) 1069, 1258
BstKTI GATC 6 cut(s) 7, 272, 310, 425, 800, 865
BstMAI GTCTC 3 cut(s) 682, 710, 1363
BstMBI GATC 6 cut(s) 4, 269, 307, 422, 797, 862
BstMWI GCNNNNNNNGC 2 cut(s) 491, 1182
BstNI CCWGG 1 cut(s) 243
BstNSI RCATGY 2 cut(s) 1026, 1237
BstSCI CCNGG 3 cut(s) 126, 241, 1185
BstSNI TACGTA 1 cut(s) 253
BstUI CGCG 4 cut(s) 154, 896, 1067, 1334
BstV1I GCAGC 1 cut(s) 101
BstV2I GAAGAC 1 cut(s) 1051
BstX2I RGATCY 3 cut(s) 4, 422, 862
BstYI RGATCY 3 cut(s) 4, 422, 862
BstZ17I GTATAC 1 cut(s) 1231
BsuI GTATCC 2 cut(s) 174, 265
BsuRI GGCC 3 cut(s) 246, 474, 871
BtgZI GCGATG 1 cut(s) 1048
BtrI CACGTC 1 cut(s) 684
BtsCI GGATG 3 cut(s) 742, 763, 1195
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 3 cut(s) 60, 1147, 1251
BveI ACCTGC 1 cut(s) 1148
CaiI CAGNNNCTG 1 cut(s) 374
CfoI GCGC 2 cut(s) 1069, 1258
Cfr10I RCCGGY 4 cut(s) 217, 246, 470, 619
Cfr13I GGNCC 1 cut(s) 869
CseI GACGC 1 cut(s) 101
Csp6I GTAC 4 cut(s) 250, 380, 593, 1237
CspAI ACCGGT 2 cut(s) 217, 619
CviAII CATG 5 cut(s) 389, 515, 848, 1023, 1234
CviQI GTAC 4 cut(s) 250, 380, 593, 1237
DdeI CTNAG 5 cut(s) 48, 108, 367, 812, 1201
DpnI GATC 6 cut(s) 6, 271, 309, 424, 799, 864
DpnII GATC 6 cut(s) 4, 269, 307, 422, 797, 862
DriI GACNNNNNGTC 1 cut(s) 682
EaeI YGGCCR 1 cut(s) 472
Eam1105I GACNNNNNGTC 1 cut(s) 682
Eco105I TACGTA 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 1165
EcoRII CCWGG 1 cut(s) 241
FaeI CATG 5 cut(s) 392, 518, 851, 1026, 1237
FaqI GGGAC 2 cut(s) 110, 836
FatI CATG 5 cut(s) 388, 514, 847, 1022, 1233
FauI CCCGC 3 cut(s) 529, 889, 1053
FblI GTMKAC 3 cut(s) 157, 506, 1230
Fnu4HI GCNGC 3 cut(s) 87, 90, 1335
FokI GGATG 3 cut(s) 749, 750, 1202
Fsp4HI GCNGC 3 cut(s) 87, 90, 1335
FspBI CTAG 2 cut(s) 278, 447
GlaI GCGC 2 cut(s) 1068, 1257
GluI GCNGC 3 cut(s) 87, 90, 1335
GsuI CTGGAG 1 cut(s) 288
HaeIII GGCC 3 cut(s) 246, 474, 871
HapII CCGG 9 cut(s) 128, 218, 224, 230, 236, 247, 471, 620, 1187
HgaI GACGC 1 cut(s) 101
HhaI GCGC 2 cut(s) 1069, 1258
Hin1II CATG 5 cut(s) 392, 518, 851, 1026, 1237
Hin6I GCGC 2 cut(s) 1067, 1256
HinP1I GCGC 2 cut(s) 1067, 1256
HincII GTYRAC 1 cut(s) 468
HindII GTYRAC 1 cut(s) 468
HindIII AAGCTT 1 cut(s) 1275
HinfI GANTC 7 cut(s) 32, 188, 295, 301, 325, 371, 1137
HpaI GTTAAC 1 cut(s) 468
HpaII CCGG 9 cut(s) 128, 218, 224, 230, 236, 247, 471, 620, 1187
HphI GGTGA 1 cut(s) 1034
Hpy166II GTNNAC 5 cut(s) 158, 468, 507, 556, 1231
Hpy188I TCNGA 8 cut(s) 37, 187, 300, 319, 330, 370, 376, 1219
Hpy188III TCNNGA 5 cut(s) 305, 687, 767, 878, 978
Hpy8I GTNNAC 5 cut(s) 158, 468, 507, 556, 1231
Hpy99I CGWCG 4 cut(s) 41, 44, 208, 688
HpyAV CCTTC 2 cut(s) 114, 1136
HpyCH4III ACNGT 6 cut(s) 96, 162, 176, 352, 693, 1223
HpyCH4IV ACGT 6 cut(s) 252, 558, 683, 780, 1039, 1326
HpyCH4V TGCA 8 cut(s) 15, 433, 617, 681, 847, 1032, 1157, 1176
HpyF10VI GCNNNNNNNGC 2 cut(s) 491, 1182
HpyF3I CTNAG 5 cut(s) 48, 108, 367, 812, 1201
HpySE526I ACGT 6 cut(s) 252, 558, 683, 780, 1039, 1326
Hsp92II CATG 5 cut(s) 392, 518, 851, 1026, 1237
HspAI GCGC 2 cut(s) 1067, 1256
KspAI GTTAAC 1 cut(s) 468
Kzo9I GATC 6 cut(s) 4, 269, 307, 422, 797, 862
LmnI GCTCC 2 cut(s) 133, 360
Lsp1109I GCAGC 1 cut(s) 101
LweI GCATC 2 cut(s) 372, 1041
MaeI CTAG 2 cut(s) 278, 447
MaeII ACGT 6 cut(s) 252, 558, 683, 780, 1039, 1326
MaeIII GTNAC 5 cut(s) 55, 487, 776, 1040, 1145
MalI GATC 6 cut(s) 6, 271, 309, 424, 799, 864
MboI GATC 6 cut(s) 4, 269, 307, 422, 797, 862
MboII GAAGA 5 cut(s) 390, 515, 598, 1051, 1087
MflI RGATCY 3 cut(s) 4, 422, 862
MluCI AATT 5 cut(s) 16, 99, 772, 839, 873
MlyI GAGTC 3 cut(s) 26, 295, 334
MmeI TCCRAC 3 cut(s) 60, 323, 727
MnlI CCTC 9 cut(s) 124, 177, 292, 393, 436, 701, 727, 976, 1216
MseI TTAA 6 cut(s) 71, 467, 624, 756, 1263, 1296
MslI CAYNNNNRTG 3 cut(s) 612, 852, 1021
MspI CCGG 9 cut(s) 128, 218, 224, 230, 236, 247, 471, 620, 1187
MspR9I CCNGG 3 cut(s) 128, 243, 1187
Mva1269I GAATGC 1 cut(s) 958
MvaI CCWGG 1 cut(s) 243
MvnI CGCG 4 cut(s) 154, 896, 1067, 1334
MwoI GCNNNNNNNGC 2 cut(s) 491, 1182
NciI CCSGG 2 cut(s) 128, 1187
NdeII GATC 6 cut(s) 4, 269, 307, 422, 797, 862
NlaIII CATG 5 cut(s) 392, 518, 851, 1026, 1237
NlaIV GGNNCC 7 cut(s) 178, 222, 228, 234, 240, 424, 987
NmuCI GTSAC 3 cut(s) 55, 1040, 1145
NspI RCATGY 2 cut(s) 1026, 1237
NspV TTCGAA 1 cut(s) 1140
PciI ACATGT 1 cut(s) 1233
PctI GAATGC 1 cut(s) 958
PfeI GAWTC 4 cut(s) 188, 295, 371, 1137
PflFI GACNNNGTC 1 cut(s) 1055
PfoI TCCNGGA 1 cut(s) 126
PinAI ACCGGT 2 cut(s) 217, 619
PkrI GCNGC 3 cut(s) 88, 91, 1336
PleI GAGTC 3 cut(s) 26, 295, 333
PpsI GAGTC 3 cut(s) 26, 295, 333
Ppu21I YACGTR 2 cut(s) 253, 559
PscI ACATGT 1 cut(s) 1233
Psp6I CCWGG 1 cut(s) 241
PspGI CCWGG 1 cut(s) 241
PspN4I GGNNCC 7 cut(s) 178, 222, 228, 234, 240, 424, 987
PspPI GGNCC 1 cut(s) 869
PstNI CAGNNNCTG 1 cut(s) 374
PsuI RGATCY 3 cut(s) 4, 422, 862
PsyI GACNNNGTC 1 cut(s) 1055
RsaI GTAC 4 cut(s) 251, 381, 594, 1238
RsaNI GTAC 4 cut(s) 250, 380, 593, 1237
RseI CAYNNNNRTG 3 cut(s) 612, 852, 1021
SaqAI TTAA 6 cut(s) 71, 467, 624, 756, 1263, 1296
SatI GCNGC 3 cut(s) 87, 90, 1335
Sau3AI GATC 6 cut(s) 4, 269, 307, 422, 797, 862
Sau96I GGNCC 1 cut(s) 869
SchI GAGTC 3 cut(s) 26, 295, 334
ScrFI CCNGG 3 cut(s) 128, 243, 1187
SfaNI GCATC 2 cut(s) 372, 1041
SfuI TTCGAA 1 cut(s) 1140
SmiMI CAYNNNNRTG 3 cut(s) 612, 852, 1021
SnaBI TACGTA 1 cut(s) 253
Sse9I AATT 5 cut(s) 16, 99, 772, 839, 873
SsiI CCGC 6 cut(s) 87, 152, 536, 896, 1046, 1334
SspMI CTAG 2 cut(s) 278, 447
StyD4I CCNGG 3 cut(s) 126, 241, 1185
TaaI ACNGT 6 cut(s) 96, 162, 176, 352, 693, 1223
TaiI ACGT 6 cut(s) 255, 561, 686, 783, 1042, 1329
TaqI TCGA 4 cut(s) 458, 686, 977, 1140
TasI AATT 5 cut(s) 16, 99, 772, 839, 873
TauI GCSGC 2 cut(s) 89, 1337
TfiI GAWTC 4 cut(s) 188, 295, 371, 1137
Tru1I TTAA 6 cut(s) 71, 467, 624, 756, 1263, 1296
Tru9I TTAA 6 cut(s) 71, 467, 624, 756, 1263, 1296
TscAI CASTG 3 cut(s) 60, 1154, 1258
TseFI GTSAC 3 cut(s) 55, 1040, 1145
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 3 cut(s) 55, 1040, 1145
TspDTI ATGAA 6 cut(s) 509, 752, 753, 864, 1051, 1296
TspRI CASTG 3 cut(s) 60, 1154, 1258
Tth111I GACNNNGTC 1 cut(s) 1055
XapI RAATTY 1 cut(s) 839
XceI RCATGY 2 cut(s) 1026, 1237
XmiI GTMKAC 3 cut(s) 157, 506, 1230
XspI CTAG 2 cut(s) 278, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.