Basic Information

Type: gene

Biological Identity

Species

prunus_persica

Chromosome

Pp04

Orthogroup ID

OG0008102

Physical Location & Seq

Genomic Coordinates Forward (+)

12016983 .. 12021698

Gene Structure 4716 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Prupe.4G196900.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGATCTTCTCTGCAACGCATACTCAAACGCCTCAGACAACGAAGAAGAGGAAGAAGAATCGGCTAAACAACCCAGACCCGAAAACTTCACGCCCCCGAATTCGAAGCGGCTCAAACCCGTTAACCCATTTACACATACTAAAACTCAGAATCCTCTGTCGGGCTCAACCCAGTTCCCGGCCCGCCATATAGAAGCTCCGGTTCCTGGTAGATACGTATCCAAGAGAGAGCGCTCTCTCTTGGGTTCCCAACCTACCGCCTCCGACCCGAACCCGGATGCTTTGCTGCTGACAACCTCTCCTGTTTTTGGGAGTATTTCGGATTCTGATGTTCCTCGTGATATTAAGTCATTGTTGAGAAACAAAGCAAAAAAGGGTCGTGCACAGCTTGGCCAGATGCCTCAAAGAATGTCTGTGCCTCTAAGCAGACATACCAAAGCTGTTAATGCTGTACATTGGTCACCAACTCATGCTCATCTTCTTGCATCTGCTTCGATGGATCAAACAATCTGCATATGGAATGTGTGGAGCAGTGATCAGAAGGTAGCCCGTGTTTTGAATTTTCACAATGCTGCTATCAAAGATGTGAAATGGTCGGGGCAAGGGTTGTTTGTGCTTTCTTGTGGATATGACTCCTCATCAAAGCTAGTGGATGTTGAAAGGGGGATAGAGACTCAGGTTTATAAGGAGGATCAAGTTGTTAGTGTTGTTAAATTCCATCCTGATAATTTCAACCTCTTCGTTTCTGGGGGGTCCAAAGGCGGTCTGAGGTTGTGGGATGTTAGAAATGGCAAGGTGGTCCATGAATACGTTCGAGGTCACGATCCCATCCTTGATGTTGAATTCACCACGAATGGCAAGCAGATTATATCGTCTAGTGATGTATCTGGACGCAACCTTAGTGAAAATTCTATTGTTGTTTGGGATGTTTCACGACAAGTTCCACTCTCTAATCAGGTTTATGTGGAAGCCTACACATGTCCCTGTGTGAGATGCCACACATTTGAACCATTTTTCGTTGCCCAGTCAAATGGTAATTATATTGCAATCTTCTCTTCAATCCCCCCCTTCAAGCTGAACAAGTACAAGAGGTATGAAAGCCATGGGGTATCAGGATTTCCCATTAAATGCGTGTTTAGCGTGGATGGGGAAAAGCTAATCTCGGGCTCCTCTGATGGGTCTATATACTTCTACGACTATAGGTCTTCTGAGCTTGTCAGAAAAATCAAGGCATATGAGCAGGCTTGCATTGACATTGCTATCCACCCCAGCATACCTAATGTGATTGCTTCATGCAGTTGGAATGGAGAGGTTTCAGTGTTCGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

443

Amino Acids

49.16

Weight (kDa)

8.0

Isoelectric Point (pI)

43.67

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008102)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54520
fragaria_vesca	FvH4_3g21450 FvH4_3g21450 FvH4_3g21450 FvH4_3g21450 FvH4_3g21450
malus_domestica	MD03G1215900.v1.1 MD11G1230500.v1.1
prunus_persica	Prupe.4G196900_v2.0.a1 Prupe.4G196900_v2.0.a1 Prupe.4G196900_v2.0.a1 Prupe.4G196900_v2.0.a1 Prupe.4G196900_v2.0.a1
pyrus_communis	pycom03g16420
rosa_chinensis	RchiOBHm_Chr5g0036881
rosa_laevigata	RLG00000033731
rosa_multiflora	Rmu_sc0007663.1_g000001
rosa_roxburghii	Rroxscaffold_1G00044080
rosa_rugosa	Rorug05G0158500
rosa_samantha	Rh5AG251000 Rh5BG252400 Rh5CG285000 Rh5DG261000
rosa_wichuraiana	Rw5G023070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	684
AciI	CCGC	4 cut(s)	109, 184, 258, 762
AclWI	GGATC	4 cut(s)	12, 507, 699, 818
AcoI	YGGCCR	1 cut(s)	391
AcsI	RAATTY	5 cut(s)	100, 559, 713, 842, 907
AfaI	GTAC	2 cut(s)	453, 1085
AfeI	AGCGCT	1 cut(s)	233
AfiI	CCNNNNNNNGG	6 cut(s)	161, 178, 206, 274, 308, 1176
AflIII	ACRYGT	1 cut(s)	977
AgsI	TTSAA	7 cut(s)	559, 659, 733, 842, 1007, 1059, 1072
AhdI	GACNNNNNGTC	1 cut(s)	1201
AjnI	CCWGG	1 cut(s)	205
AluBI	AGCT	7 cut(s)	197, 388, 440, 646, 1075, 1156, 1213
AluI	AGCT	7 cut(s)	197, 388, 440, 646, 1075, 1156, 1213
Alw21I	GWGCWC	1 cut(s)	385
Alw26I	GTCTC	1 cut(s)	665
Alw44I	GTGCAC	1 cut(s)	381
AlwI	GGATC	4 cut(s)	12, 507, 699, 818
Ama87I	CYCGRG	1 cut(s)	1162
Aor51HI	AGCGCT	1 cut(s)	233
AoxI	GGCC	2 cut(s)	180, 391
ApaLI	GTGCAC	1 cut(s)	381
ApeKI	GCWGC	2 cut(s)	286, 572
ApoI	RAATTY	5 cut(s)	100, 559, 713, 842, 907
Asp700I	GAANNNNTTC	1 cut(s)	810
AspLEI	GCGC	1 cut(s)	234
AspS9I	GGNCC	3 cut(s)	181, 753, 799
AsuC2I	CCSGG	2 cut(s)	179, 275
AsuHPI	GGTGA	2 cut(s)	453, 838
AsuII	TTCGAA	1 cut(s)	104
AvaI	CYCGRG	1 cut(s)	1162
AvaII	GGWCC	2 cut(s)	753, 799
BaeGI	GKGCMC	1 cut(s)	385
BalI	TGGCCA	1 cut(s)	393
BanII	GRGCYC	2 cut(s)	167, 1169
BauI	CACGAG	1 cut(s)	336
BbsI	GAAGAC	1 cut(s)	1197
Bbv12I	GWGCWC	1 cut(s)	385
BbvI	GCAGC	2 cut(s)	273, 559
BccI	CCATC	5 cut(s)	490, 726, 836, 1139, 1169
BcgI	CGANNNNNNTGC	2 cut(s)	474, 508
BciT130I	CCWGG	1 cut(s)	207
BciVI	GTATCC	1 cut(s)	229
BclI	TGATCA	1 cut(s)	535
BcnI	CCSGG	2 cut(s)	179, 275
BcoDI	GTCTC	1 cut(s)	665
BfaI	CTAG	2 cut(s)	647, 876
BfmI	CTRYAG	1 cut(s)	1198
BfoI	RGCGCY	1 cut(s)	235
BfuI	GTATCC	1 cut(s)	229
BisI	GCNGC	3 cut(s)	110, 287, 573
BlsI	GCNGC	3 cut(s)	111, 288, 574
Bme1390I	CCNGG	3 cut(s)	179, 207, 275
Bme18I	GGWCC	2 cut(s)	753, 799
BmeRI	GACNNNNNGTC	1 cut(s)	1201
BmeT110I	CYCGRG	1 cut(s)	1162
BmgT120I	GGNCC	3 cut(s)	181, 753, 799
BmiI	GGNNCC	4 cut(s)	204, 247, 754, 1168
BmrFI	CCNGG	3 cut(s)	179, 207, 275
BmrI	ACTGGG	2 cut(s)	166, 1018
BmsI	GCATC	4 cut(s)	268, 387, 494, 983
BmuI	ACTGGG	2 cut(s)	166, 1018
BpiI	GAAGAC	1 cut(s)	1197
Bpu14I	TTCGAA	1 cut(s)	104
BpuMI	CCSGG	2 cut(s)	179, 275
BsaAI	YACGTR	1 cut(s)	217
BsaBI	GATNNNNATC	1 cut(s)	217
BsaJI	CCNNGG	1 cut(s)	1102
BsaWI	WCCGGW	1 cut(s)	199
BsaXI	ACNNNNNCTCC	2 cut(s)	283, 313
Bsc4I	CCNNNNNNNGG	6 cut(s)	161, 178, 206, 274, 308, 1176
Bse1I	ACTGG	2 cut(s)	172, 1024
Bse3DI	GCAATG	1 cut(s)	1254
Bse8I	GATNNNNATC	1 cut(s)	217
BseBI	CCWGG	1 cut(s)	207
BseDI	CCNNGG	1 cut(s)	1102
BseGI	GGATG	7 cut(s)	283, 658, 718, 784, 828, 931, 1150
BseJI	GATNNNNATC	1 cut(s)	217
BseLI	CCNNNNNNNGG	6 cut(s)	161, 178, 206, 274, 308, 1176
BseMI	GCAATG	1 cut(s)	1254
BseMII	CTCAG	5 cut(s)	48, 161, 689, 758, 1200
BseNI	ACTGG	2 cut(s)	172, 1024
BseRI	GAGGAG	2 cut(s)	625, 1159
BseSI	GKGCMC	1 cut(s)	385
BseXI	GCAGC	2 cut(s)	273, 559
BseYI	CCCAGC	1 cut(s)	1268
BshFI	GGCC	2 cut(s)	182, 393
BsiHKAI	GWGCWC	1 cut(s)	385
BsiHKCI	CYCGRG	1 cut(s)	1162
BsiSI	CCGG	3 cut(s)	179, 200, 275
BslFI	GGGAC	1 cut(s)	966
BslI	CCNNNNNNNGG	6 cut(s)	161, 178, 206, 274, 308, 1176
BsmAI	GTCTC	1 cut(s)	665
BsmFI	GGGAC	1 cut(s)	966
BsnI	GGCC	2 cut(s)	182, 393
BsoBI	CYCGRG	1 cut(s)	1162
Bsp119I	TTCGAA	1 cut(s)	104
Bsp1286I	GDGCHC	3 cut(s)	167, 385, 1169
Bsp1407I	TGTACA	1 cut(s)	451
Bsp143I	GATC	5 cut(s)	4, 499, 535, 691, 823
Bsp19I	CCATGG	1 cut(s)	1102
BspACI	CCGC	4 cut(s)	109, 184, 258, 762
BspANI	GGCC	2 cut(s)	182, 393
BspCNI	CTCAG	5 cut(s)	47, 160, 688, 759, 1201
BspLI	GGNNCC	4 cut(s)	204, 247, 754, 1168
BspPI	GGATC	4 cut(s)	12, 507, 699, 818
BspT104I	TTCGAA	1 cut(s)	104
BsrDI	GCAATG	1 cut(s)	1254
BsrGI	TGTACA	1 cut(s)	451
BsrI	ACTGG	2 cut(s)	172, 1024
BssECI	CCNNGG	1 cut(s)	1102
BssMI	GATC	5 cut(s)	4, 499, 535, 691, 823
BssSI	CACGAG	1 cut(s)	336
BssT1I	CCWWGG	1 cut(s)	1102
Bst2BI	CACGAG	1 cut(s)	336
Bst2UI	CCWGG	1 cut(s)	207
Bst6I	CTCTTC	3 cut(s)	42, 743, 1060
BstAUI	TGTACA	1 cut(s)	451
BstBAI	YACGTR	1 cut(s)	217
BstBI	TTCGAA	1 cut(s)	104
BstC8I	GCNNGC	4 cut(s)	184, 860, 1242, 1246
BstDEI	CTNAG	7 cut(s)	34, 147, 422, 675, 767, 899, 1209
BstDSI	CCRYGG	1 cut(s)	1102
BstEII	GGTNACC	1 cut(s)	459
BstF5I	GGATG	7 cut(s)	283, 658, 718, 784, 828, 931, 1150
BstH2I	RGCGCY	1 cut(s)	235
BstHHI	GCGC	1 cut(s)	234
BstKTI	GATC	5 cut(s)	7, 502, 538, 694, 826
BstMAI	GTCTC	1 cut(s)	665
BstMBI	GATC	5 cut(s)	4, 499, 535, 691, 823
BstMWI	GCNNNNNNNGC	2 cut(s)	446, 1137
BstNI	CCWGG	1 cut(s)	207
BstNSI	RCATGY	1 cut(s)	981
BstPI	GGTNACC	1 cut(s)	459
BstSCI	CCNGG	3 cut(s)	177, 205, 273
BstSFI	CTRYAG	1 cut(s)	1198
BstSLI	GKGCMC	1 cut(s)	385
BstSNI	TACGTA	1 cut(s)	217
BstV1I	GCAGC	2 cut(s)	273, 559
BstV2I	GAAGAC	1 cut(s)	1197
BstX2I	RGATCY	1 cut(s)	4
BstXI	CCANNNNNNTGG	1 cut(s)	1031
BstYI	RGATCY	1 cut(s)	4
BsuI	GTATCC	1 cut(s)	229
BsuRI	GGCC	2 cut(s)	182, 393
BtgI	CCRYGG	1 cut(s)	1102
BtsCI	GGATG	7 cut(s)	283, 658, 718, 784, 828, 931, 1150
BtsI	GCAGTG	1 cut(s)	538
BtsIMutI	CAGTG	2 cut(s)	538, 1323
Cac8I	GCNNGC	4 cut(s)	184, 860, 1242, 1246
CfoI	GCGC	1 cut(s)	234
Cfr13I	GGNCC	3 cut(s)	181, 753, 799
CseI	GACGC	1 cut(s)	900
Csp6I	GTAC	2 cut(s)	452, 1084
CspCI	CAANNNNNGTGG	2 cut(s)	630, 665
CviAII	CATG	5 cut(s)	470, 803, 978, 1103, 1293
CviQI	GTAC	2 cut(s)	452, 1084
DdeI	CTNAG	7 cut(s)	34, 147, 422, 675, 767, 899, 1209
DpnI	GATC	5 cut(s)	6, 501, 537, 693, 825
DpnII	GATC	5 cut(s)	4, 499, 535, 691, 823
DriI	GACNNNNNGTC	1 cut(s)	1201
EaeI	YGGCCR	1 cut(s)	391
Eam1104I	CTCTTC	3 cut(s)	42, 743, 1060
Eam1105I	GACNNNNNGTC	1 cut(s)	1201
EarI	CTCTTC	3 cut(s)	42, 743, 1060
Eco105I	TACGTA	1 cut(s)	217
Eco130I	CCWWGG	1 cut(s)	1102
Eco24I	GRGCYC	2 cut(s)	167, 1169
Eco47I	GGWCC	2 cut(s)	753, 799
Eco47III	AGCGCT	1 cut(s)	233
Eco88I	CYCGRG	1 cut(s)	1162
Eco91I	GGTNACC	1 cut(s)	459
EcoO65I	GGTNACC	1 cut(s)	459
EcoRI	GAATTC	2 cut(s)	100, 842
EcoRII	CCWGG	1 cut(s)	205
EcoT14I	CCWWGG	1 cut(s)	1102
EcoT38I	GRGCYC	2 cut(s)	167, 1169
ErhI	CCWWGG	1 cut(s)	1102
FaeI	CATG	5 cut(s)	473, 806, 981, 1106, 1296
FaqI	GGGAC	1 cut(s)	966
FatI	CATG	5 cut(s)	469, 802, 977, 1102, 1292
FauI	CCCGC	1 cut(s)	191
FauNDI	CATATG	2 cut(s)	515, 1234
FbaI	TGATCA	1 cut(s)	535
Fnu4HI	GCNGC	3 cut(s)	110, 287, 573
FokI	GGATG	7 cut(s)	290, 665, 705, 791, 815, 938, 1157
FriOI	GRGCYC	2 cut(s)	167, 1169
Fsp4HI	GCNGC	3 cut(s)	110, 287, 573
FspBI	CTAG	2 cut(s)	647, 876
GlaI	GCGC	1 cut(s)	233
GluI	GCNGC	3 cut(s)	110, 287, 573
GsaI	CCCAGC	1 cut(s)	1272
HaeII	RGCGCY	1 cut(s)	235
HaeIII	GGCC	2 cut(s)	182, 393
HapII	CCGG	3 cut(s)	179, 200, 275
HgaI	GACGC	1 cut(s)	900
HhaI	GCGC	1 cut(s)	234
Hin1II	CATG	5 cut(s)	473, 806, 981, 1106, 1296
Hin6I	GCGC	1 cut(s)	232
HinP1I	GCGC	1 cut(s)	232
HincII	GTYRAC	1 cut(s)	124
HindII	GTYRAC	1 cut(s)	124
HinfI	GANTC	5 cut(s)	59, 151, 323, 632, 673
HpaI	GTTAAC	1 cut(s)	124
HpaII	CCGG	3 cut(s)	179, 200, 275
HphI	GGTGA	2 cut(s)	453, 838
Hpy166II	GTNNAC	2 cut(s)	124, 383
Hpy188III	TCNNGA	6 cut(s)	338, 722, 821, 888, 933, 1113
Hpy8I	GTNNAC	2 cut(s)	124, 383
HpyAV	CCTTC	2 cut(s)	535, 1078
HpyCH4IV	ACGT	2 cut(s)	216, 810
HpyCH4V	TGCA	7 cut(s)	15, 383, 485, 513, 1046, 1248, 1296
HpyF10VI	GCNNNNNNNGC	2 cut(s)	446, 1137
HpyF3I	CTNAG	7 cut(s)	34, 147, 422, 675, 767, 899, 1209
HpySE526I	ACGT	2 cut(s)	216, 810
Hsp92II	CATG	5 cut(s)	473, 806, 981, 1106, 1296
HspAI	GCGC	1 cut(s)	232
Ksp22I	TGATCA	1 cut(s)	535
KspAI	GTTAAC	1 cut(s)	124
Kzo9I	GATC	5 cut(s)	4, 499, 535, 691, 823
LmnI	GCTCC	3 cut(s)	202, 528, 1172
Lsp1109I	GCAGC	2 cut(s)	273, 559
LweI	GCATC	4 cut(s)	268, 387, 494, 983
MaeI	CTAG	2 cut(s)	647, 876
MaeII	ACGT	2 cut(s)	216, 810
MaeIII	GTNAC	2 cut(s)	459, 818
MalI	GATC	5 cut(s)	6, 501, 537, 693, 825
MboI	GATC	5 cut(s)	4, 499, 535, 691, 823
MboII	GAAGA	9 cut(s)	56, 59, 65, 68, 470, 730, 1042, 1047, 1197
MflI	RGATCY	1 cut(s)	4
MhlI	GDGCHC	3 cut(s)	167, 385, 1169
MlsI	TGGCCA	1 cut(s)	393
MluCI	AATT	7 cut(s)	100, 559, 713, 727, 842, 907, 1036
MluNI	TGGCCA	1 cut(s)	393
MlyI	GAGTC	2 cut(s)	626, 667
MmeI	TCCRAC	2 cut(s)	288, 1280
Mox20I	TGGCCA	1 cut(s)	393
MroXI	GAANNNNTTC	1 cut(s)	810
MscI	TGGCCA	1 cut(s)	393
MseI	TTAA	5 cut(s)	123, 345, 444, 711, 1125
Msp20I	TGGCCA	1 cut(s)	393
MspI	CCGG	3 cut(s)	179, 200, 275
MspR9I	CCNGG	3 cut(s)	179, 207, 275
MvaI	CCWGG	1 cut(s)	207
MwoI	GCNNNNNNNGC	2 cut(s)	446, 1137
NciI	CCSGG	2 cut(s)	179, 275
NcoI	CCATGG	1 cut(s)	1102
NdeI	CATATG	2 cut(s)	515, 1234
NdeII	GATC	5 cut(s)	4, 499, 535, 691, 823
NlaIII	CATG	5 cut(s)	473, 806, 981, 1106, 1296
NlaIV	GGNNCC	4 cut(s)	204, 247, 754, 1168
NmuCI	GTSAC	2 cut(s)	459, 818
NspI	RCATGY	1 cut(s)	981
NspV	TTCGAA	1 cut(s)	104
PciI	ACATGT	1 cut(s)	977
PdmI	GAANNNNTTC	1 cut(s)	810
PfeI	GAWTC	3 cut(s)	59, 151, 323
PkrI	GCNGC	3 cut(s)	111, 288, 574
PleI	GAGTC	2 cut(s)	626, 667
PpsI	GAGTC	2 cut(s)	626, 667
Ppu21I	YACGTR	1 cut(s)	217
PscI	ACATGT	1 cut(s)	977
PsiI	TTATAA	1 cut(s)	684
Psp6I	CCWGG	1 cut(s)	205
PspEI	GGTNACC	1 cut(s)	459
PspFI	CCCAGC	1 cut(s)	1268
PspGI	CCWGG	1 cut(s)	205
PspN4I	GGNNCC	4 cut(s)	204, 247, 754, 1168
PspPI	GGNCC	3 cut(s)	181, 753, 799
PsuI	RGATCY	1 cut(s)	4
RsaI	GTAC	2 cut(s)	453, 1085
RsaNI	GTAC	2 cut(s)	452, 1084
SaqAI	TTAA	5 cut(s)	123, 345, 444, 711, 1125
SatI	GCNGC	3 cut(s)	110, 287, 573
Sau3AI	GATC	5 cut(s)	4, 499, 535, 691, 823
Sau96I	GGNCC	3 cut(s)	181, 753, 799
SchI	GAGTC	2 cut(s)	626, 667
ScrFI	CCNGG	3 cut(s)	179, 207, 275
SduI	GDGCHC	3 cut(s)	167, 385, 1169
SfaNI	GCATC	4 cut(s)	268, 387, 494, 983
SfcI	CTRYAG	1 cut(s)	1198
SfuI	TTCGAA	1 cut(s)	104
SinI	GGWCC	2 cut(s)	753, 799
SnaBI	TACGTA	1 cut(s)	217
Sse9I	AATT	7 cut(s)	100, 559, 713, 727, 842, 907, 1036
SsiI	CCGC	4 cut(s)	109, 184, 258, 762
SspMI	CTAG	2 cut(s)	647, 876
StyD4I	CCNGG	3 cut(s)	177, 205, 273
StyI	CCWWGG	1 cut(s)	1102
TaiI	ACGT	2 cut(s)	219, 813
TaqI	TCGA	4 cut(s)	104, 494, 814, 1323
TasI	AATT	7 cut(s)	100, 559, 713, 727, 842, 907, 1036
TatI	WGTACW	2 cut(s)	451, 1083
TauI	GCSGC	1 cut(s)	112
TfiI	GAWTC	3 cut(s)	59, 151, 323
Tru1I	TTAA	5 cut(s)	123, 345, 444, 711, 1125
Tru9I	TTAA	5 cut(s)	123, 345, 444, 711, 1125
TscAI	CASTG	2 cut(s)	538, 1323
TseFI	GTSAC	2 cut(s)	459, 818
TseI	GCWGC	2 cut(s)	286, 572
Tsp45I	GTSAC	2 cut(s)	459, 818
TspDTI	ATGAA	3 cut(s)	819, 1110, 1281
TspRI	CASTG	2 cut(s)	538, 1323
VneI	GTGCAC	1 cut(s)	381
VpaK11BI	GGWCC	2 cut(s)	753, 799
XapI	RAATTY	5 cut(s)	100, 559, 713, 842, 907
XceI	RCATGY	1 cut(s)	981
XmnI	GAANNNNTTC	1 cut(s)	810
XspI	CTAG	2 cut(s)	647, 876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.