Rh5CG285000

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008102
Physical Location & Seq
Forward (+)
31230910 .. 31235688
4779 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG285000.1

Sequence Viewer

Length: 996 bp
ATGGACCTTCTCTGCAACGCATACTCAAACGCCTCGGACAACGACGACGAAGAAGAAGAAGAAGCTCGACCACTTAAACAACCGAGACCCGAAACATCCACACCCCAGAATTGGAAGCAACACAAACCCAATAACCCATTTCAGTATACCAACACTTACCATCCTCCGCCGGCCGCAACCCAGTTTCCGGTCATCCGTACTGAGACCCAAGTCCCTGGTAGATACATATCCAAGAGAGAGCGGGCTCTTTTGGGCTCCCAGCCCAGCGTCCCCGACCCGAACCCAAACCCGAATACCCTGGCCACCACCTCCCCTGTGTTGGGGAGTATTTCGGATTCTGATGTTCGTCGCGATATTGTGTCATTGTTGAGAAATAAAGCGAAGGGCTGTTCAAAGCCTGGAAAAATGCCTGAAAGAATGTCTGTGGGTCTAAATAGGCATACCAAAGCTGTCAATGCGGTACATTGGTCACCTAGTCATGCTCATCTTCTAGCCTCTGCTTCGATGGATCACACAATCTGCATATGGAGTGTGTGGAACAGTGACCAGAAGGTAGCCCGTGTGTTGAATTTTCACAATGCAGCAGTTAAAGATGTAAAATGGTCACGGGATGGATTGTTTGTGCTTTCTTGTGGATACGACTCCTCTTCAAGGTTAGTGGATGTTGAAAAGGGGATAGAGACTCAAGTTTTTAAGGAGGATCAAGTTGTCAGTGTTGTTAAGTTCCATCCCGACAATTCAAAACTCTTCATTACTGGGGGATCAAGAGGCAGCCTCAAGCTGTGGGATGTTAGAAATGGCAAGGTGGTCCATGACTACATTCGAGGTCATGATCCTATCCTTGATGTCGAATTCACCAAGAATGGCAAGCAGATTATTTCCTCGAGTGATGTATCTGGACGAAACCTTAGTGAGAATTCTATTGTTGTTTGGGATGTTTCACGAGAAGTTCCTCTCTCTAATCAGGTAATAGACTCATTCCAATTATTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

331

Amino Acids

37.0

Weight (kDa)

7.74

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 138 - 272 5e-14 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR35_TULP_N PF24797 139 - 271 4.8e-06 WDR35/TULP4 N-terminal
Beta-prop_WDR3_1st PF25173 139 - 231 1.4e-10 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 140 - 298 2.4e-23 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 141 - 181 1.1e-07 THOC3 beta-propeller domain
Beta-prop_EML PF23409 142 - 299 3.8e-07 Echinoderm microtubule-associated protein first beta-propeller
WD40 PF00400 144 - 177 3.3e-07 WD domain, G-beta repeat
EIF3I PF24805 144 - 251 2.1e-07 EIF3I
Beta-prop_WDR5 PF25175 144 - 297 1.1e-26 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 147 - 249 7.4e-06 Aladin seven-bladed propeller
WDR55 PF24796 149 - 297 2.5e-19 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 159 - 251 4.8e-07 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 182 - 299 2.9e-09 G protein beta WD-40 repeat protein
WD40_CDC20-Fz PF24807 183 - 314 3e-12 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 192 - 315 2.9e-16 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 219 - 296 1.9e-06 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 233 - 317 5.9e-06 WDR3 first beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 241
AccI GTMKAC 1 cut(s) 146
AccII CGCG 1 cut(s) 351
AciI CCGC 4 cut(s) 167, 174, 241, 458
AclWI GGATC 4 cut(s) 516, 708, 769, 827
AcoI YGGCCR 2 cut(s) 171, 300
AcsI RAATTY 3 cut(s) 568, 851, 916
AfaI GTAC 2 cut(s) 199, 462
AfiI CCNNNNNNNGG 5 cut(s) 111, 187, 319, 320, 651
AgsI TTSAA 5 cut(s) 393, 568, 651, 668, 741
AjnI CCWGG 3 cut(s) 214, 297, 397
AluBI AGCT 3 cut(s) 65, 449, 781
AluI AGCT 3 cut(s) 65, 449, 781
Alw26I GTCTC 3 cut(s) 79, 197, 674
AlwI GGATC 4 cut(s) 516, 708, 769, 827
Ama87I CYCGRG 1 cut(s) 883
AoxI GGCC 2 cut(s) 171, 300
ApeKI GCWGC 2 cut(s) 581, 771
ApoI RAATTY 3 cut(s) 568, 851, 916
AspS9I GGNCC 2 cut(s) 4, 808
AsuHPI GGTGA 2 cut(s) 462, 847
AvaI CYCGRG 1 cut(s) 883
AvaII GGWCC 2 cut(s) 4, 808
BaeI ACNNNNGTAYC 2 cut(s) 452, 485
BalI TGGCCA 1 cut(s) 302
BanII GRGCYC 2 cut(s) 247, 257
BauI CACGAG 1 cut(s) 942
BbvI GCAGC 2 cut(s) 593, 783
BccI CCATC 4 cut(s) 168, 499, 605, 735
BciT130I CCWGG 3 cut(s) 216, 299, 399
BciVI GTATCC 1 cut(s) 629
BcoDI GTCTC 3 cut(s) 79, 197, 674
BfaI CTAG 2 cut(s) 474, 491
BfuI GTATCC 1 cut(s) 629
BisI GCNGC 3 cut(s) 174, 582, 772
BlsI GCNGC 3 cut(s) 175, 583, 773
Bme1390I CCNGG 3 cut(s) 216, 299, 399
Bme18I GGWCC 2 cut(s) 4, 808
BmeT110I CYCGRG 1 cut(s) 883
BmgT120I GGNCC 2 cut(s) 4, 808
BmiI GGNNCC 1 cut(s) 256
BmrFI CCNGG 3 cut(s) 216, 299, 399
BmrI ACTGGG 2 cut(s) 175, 765
BmuI ACTGGG 2 cut(s) 175, 765
BoxI GACNNNNGTC 1 cut(s) 209
BplI GAGNNNNNCTC 2 cut(s) 759, 791
BpuEI CTTGAG 2 cut(s) 669, 761
BsaBI GATNNNNATC 1 cut(s) 226
BsaI GGTCTC 2 cut(s) 79, 197
BsaJI CCNNGG 3 cut(s) 33, 214, 297
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 5 cut(s) 111, 187, 319, 320, 651
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 2 cut(s) 181, 760
Bse8I GATNNNNATC 1 cut(s) 226
BseBI CCWGG 3 cut(s) 216, 299, 399
BseDI CCNNGG 3 cut(s) 33, 214, 297
BseGI GGATG 8 cut(s) 95, 160, 192, 616, 667, 727, 793, 940
BseJI GATNNNNATC 1 cut(s) 226
BseLI CCNNNNNNNGG 5 cut(s) 111, 187, 319, 320, 651
BseMII CTCAG 1 cut(s) 192
BseNI ACTGG 2 cut(s) 181, 760
BseRI GAGGAG 1 cut(s) 634
BseX3I CGGCCG 1 cut(s) 171
BseXI GCAGC 2 cut(s) 593, 783
BseYI CCCAGC 2 cut(s) 258, 263
Bsh1236I CGCG 1 cut(s) 351
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 2 cut(s) 173, 302
BsiEI CGRYCG 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 883
BsiSI CCGG 2 cut(s) 170, 188
BslFI GGGAC 2 cut(s) 197, 254
BslI CCNNNNNNNGG 5 cut(s) 111, 187, 319, 320, 651
BsmAI GTCTC 3 cut(s) 79, 197, 674
BsmFI GGGAC 2 cut(s) 197, 254
BsnI GGCC 2 cut(s) 173, 302
Bso31I GGTCTC 2 cut(s) 79, 197
BsoBI CYCGRG 1 cut(s) 883
Bsp1286I GDGCHC 2 cut(s) 247, 257
Bsp143I GATC 4 cut(s) 508, 700, 761, 832
Bsp68I TCGCGA 1 cut(s) 351
BspACI CCGC 4 cut(s) 167, 174, 241, 458
BspANI GGCC 2 cut(s) 173, 302
BspCNI CTCAG 1 cut(s) 193
BspFNI CGCG 1 cut(s) 351
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 4 cut(s) 516, 708, 769, 827
BspTNI GGTCTC 2 cut(s) 79, 197
BsrBI CCGCTC 1 cut(s) 241
BsrFI RCCGGY 1 cut(s) 169
BsrI ACTGG 2 cut(s) 181, 760
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 33, 214, 297
BssMI GATC 4 cut(s) 508, 700, 761, 832
BssNAI GTATAC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 942
Bst1107I GTATAC 1 cut(s) 147
Bst2BI CACGAG 1 cut(s) 942
Bst2UI CCWGG 3 cut(s) 216, 299, 399
Bst4CI ACNGT 1 cut(s) 542
Bst6I CTCTTC 2 cut(s) 652, 752
BstC8I GCNNGC 3 cut(s) 171, 243, 869
BstDEI CTNAG 2 cut(s) 201, 908
BstEII GGTNACC 1 cut(s) 468
BstENI CCTNNNNNAGG 1 cut(s) 649
BstF5I GGATG 8 cut(s) 95, 160, 192, 616, 667, 727, 793, 940
BstFNI CGCG 1 cut(s) 351
BstKTI GATC 4 cut(s) 511, 703, 764, 835
BstMAI GTCTC 3 cut(s) 79, 197, 674
BstMBI GATC 4 cut(s) 508, 700, 761, 832
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 1 cut(s) 455
BstNI CCWGG 3 cut(s) 216, 299, 399
BstPAI GACNNNNGTC 1 cut(s) 209
BstPI GGTNACC 1 cut(s) 468
BstSCI CCNGG 3 cut(s) 214, 297, 397
BstUI CGCG 1 cut(s) 351
BstV1I GCAGC 2 cut(s) 593, 783
BstXI CCANNNNNNTGG 1 cut(s) 215
BstZ17I GTATAC 1 cut(s) 147
BstZI CGGCCG 1 cut(s) 171
BsuI GTATCC 1 cut(s) 629
BsuRI GGCC 2 cut(s) 173, 302
BtsCI GGATG 8 cut(s) 95, 160, 192, 616, 667, 727, 793, 940
BtsIMutI CAGTG 2 cut(s) 547, 718
BtuMI TCGCGA 1 cut(s) 351
Cac8I GCNNGC 3 cut(s) 171, 243, 869
CciI TCATGA 1 cut(s) 829
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 4, 808
CseI GACGC 1 cut(s) 256
Csp6I GTAC 2 cut(s) 198, 461
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CviAII CATG 3 cut(s) 479, 812, 830
CviQI GTAC 2 cut(s) 198, 461
DdeI CTNAG 2 cut(s) 201, 908
DpnI GATC 4 cut(s) 510, 702, 763, 834
DpnII GATC 4 cut(s) 508, 700, 761, 832
EaeI YGGCCR 2 cut(s) 171, 300
EagI CGGCCG 1 cut(s) 171
Eam1104I CTCTTC 2 cut(s) 652, 752
EarI CTCTTC 2 cut(s) 652, 752
EciI GGCGGA 1 cut(s) 156
EclXI CGGCCG 1 cut(s) 171
Eco24I GRGCYC 2 cut(s) 247, 257
Eco31I GGTCTC 2 cut(s) 79, 197
Eco47I GGWCC 2 cut(s) 4, 808
Eco52I CGGCCG 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 883
Eco91I GGTNACC 1 cut(s) 468
EcoNI CCTNNNNNAGG 1 cut(s) 649
EcoO65I GGTNACC 1 cut(s) 468
EcoRI GAATTC 2 cut(s) 851, 916
EcoRII CCWGG 3 cut(s) 214, 297, 397
EcoT38I GRGCYC 2 cut(s) 247, 257
FaeI CATG 3 cut(s) 482, 815, 833
FaiI YATR 9 cut(s) 22, 147, 227, 441, 480, 524, 526, 813, 831
FaqI GGGAC 2 cut(s) 197, 254
FatI CATG 3 cut(s) 478, 811, 829
FauI CCCGC 1 cut(s) 234
FauNDI CATATG 1 cut(s) 524
FblI GTMKAC 1 cut(s) 146
Fnu4HI GCNGC 3 cut(s) 174, 582, 772
FokI GGATG 8 cut(s) 82, 147, 179, 623, 674, 714, 800, 947
FriOI GRGCYC 2 cut(s) 247, 257
Fsp4HI GCNGC 3 cut(s) 174, 582, 772
FspBI CTAG 2 cut(s) 474, 491
GluI GCNGC 3 cut(s) 174, 582, 772
GsaI CCCAGC 2 cut(s) 262, 267
HaeIII GGCC 2 cut(s) 173, 302
HapII CCGG 2 cut(s) 170, 188
HgaI GACGC 1 cut(s) 256
Hin1II CATG 3 cut(s) 482, 815, 833
HinfI GANTC 4 cut(s) 335, 641, 682, 974
HpaII CCGG 2 cut(s) 170, 188
HphI GGTGA 2 cut(s) 462, 847
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 3 cut(s) 37, 334, 340
Hpy188III TCNNGA 6 cut(s) 350, 731, 765, 830, 897, 942
Hpy8I GTNNAC 1 cut(s) 147
Hpy99I CGWCG 3 cut(s) 47, 50, 351
HpyAV CCTTC 3 cut(s) 17, 376, 544
HpyCH4III ACNGT 1 cut(s) 542
HpyCH4V TGCA 3 cut(s) 15, 522, 581
HpyF10VI GCNNNNNNNGC 1 cut(s) 455
HpyF3I CTNAG 2 cut(s) 201, 908
Hsp92II CATG 3 cut(s) 482, 815, 833
KroI GCCGGC 1 cut(s) 169
KroNI GCCGGC 1 cut(s) 171
Kzo9I GATC 4 cut(s) 508, 700, 761, 832
LmnI GCTCC 1 cut(s) 260
Lsp1109I GCAGC 2 cut(s) 593, 783
MaeI CTAG 2 cut(s) 474, 491
MaeIII GTNAC 3 cut(s) 468, 542, 603
MalI GATC 4 cut(s) 510, 702, 763, 834
MbiI CCGCTC 1 cut(s) 241
MboI GATC 4 cut(s) 508, 700, 761, 832
MboII GAAGA 7 cut(s) 62, 65, 68, 71, 479, 639, 739
MhlI GDGCHC 2 cut(s) 247, 257
MlsI TGGCCA 1 cut(s) 302
MluCI AATT 6 cut(s) 109, 568, 736, 851, 916, 983
MluNI TGGCCA 1 cut(s) 302
MlyI GAGTC 3 cut(s) 635, 676, 968
Mox20I TGGCCA 1 cut(s) 302
MroNI GCCGGC 1 cut(s) 169
MscI TGGCCA 1 cut(s) 302
MseI TTAA 5 cut(s) 75, 588, 693, 720, 994
Msp20I TGGCCA 1 cut(s) 302
MspI CCGG 2 cut(s) 170, 188
MspR9I CCNGG 3 cut(s) 216, 299, 399
MvaI CCWGG 3 cut(s) 216, 299, 399
MvnI CGCG 1 cut(s) 351
MwoI GCNNNNNNNGC 1 cut(s) 455
NaeI GCCGGC 1 cut(s) 171
NdeI CATATG 1 cut(s) 524
NdeII GATC 4 cut(s) 508, 700, 761, 832
NgoMIV GCCGGC 1 cut(s) 169
NlaIII CATG 3 cut(s) 482, 815, 833
NlaIV GGNNCC 1 cut(s) 256
NmuCI GTSAC 3 cut(s) 468, 542, 603
NruI TCGCGA 1 cut(s) 351
PaeR7I CTCGAG 1 cut(s) 883
PagI TCATGA 1 cut(s) 829
PdiI GCCGGC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 335
PkrI GCNGC 3 cut(s) 175, 583, 773
PleI GAGTC 3 cut(s) 635, 676, 968
PpsI GAGTC 3 cut(s) 635, 676, 968
PshAI GACNNNNGTC 1 cut(s) 209
Psp6I CCWGG 3 cut(s) 214, 297, 397
PspEI GGTNACC 1 cut(s) 468
PspFI CCCAGC 2 cut(s) 258, 263
PspGI CCWGG 3 cut(s) 214, 297, 397
PspN4I GGNNCC 1 cut(s) 256
PspPI GGNCC 2 cut(s) 4, 808
PspXI VCTCGAGB 1 cut(s) 883
RruI TCGCGA 1 cut(s) 351
RsaI GTAC 2 cut(s) 199, 462
RsaNI GTAC 2 cut(s) 198, 461
SaqAI TTAA 5 cut(s) 75, 588, 693, 720, 994
SatI GCNGC 3 cut(s) 174, 582, 772
Sau3AI GATC 4 cut(s) 508, 700, 761, 832
Sau96I GGNCC 2 cut(s) 4, 808
SchI GAGTC 3 cut(s) 635, 676, 968
ScrFI CCNGG 3 cut(s) 216, 299, 399
SduI GDGCHC 2 cut(s) 247, 257
Sfr274I CTCGAG 1 cut(s) 883
SinI GGWCC 2 cut(s) 4, 808
SlaI CTCGAG 1 cut(s) 883
SmlI CTYRAG 3 cut(s) 684, 776, 883
SmoI CTYRAG 3 cut(s) 684, 776, 883
Sse9I AATT 6 cut(s) 109, 568, 736, 851, 916, 983
SsiI CCGC 4 cut(s) 167, 174, 241, 458
SspMI CTAG 2 cut(s) 474, 491
StyD4I CCNGG 3 cut(s) 214, 297, 397
TaaI ACNGT 1 cut(s) 542
TaqI TCGA 5 cut(s) 67, 503, 823, 849, 884
TasI AATT 6 cut(s) 109, 568, 736, 851, 916, 983
TauI GCSGC 1 cut(s) 176
TfiI GAWTC 1 cut(s) 335
Tru1I TTAA 5 cut(s) 75, 588, 693, 720, 994
Tru9I TTAA 5 cut(s) 75, 588, 693, 720, 994
TscAI CASTG 2 cut(s) 547, 718
TseFI GTSAC 3 cut(s) 468, 542, 603
TseI GCWGC 2 cut(s) 581, 771
Tsp45I GTSAC 3 cut(s) 468, 542, 603
TspDTI ATGAA 1 cut(s) 739
TspGWI ACGGA 1 cut(s) 185
TspRI CASTG 2 cut(s) 547, 718
VpaK11BI GGWCC 2 cut(s) 4, 808
XagI CCTNNNNNAGG 1 cut(s) 649
XapI RAATTY 3 cut(s) 568, 851, 916
XhoI CTCGAG 1 cut(s) 883
XmiI GTMKAC 1 cut(s) 146
XspI CTAG 2 cut(s) 474, 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.