FvH4_3g21450

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008102
Physical Location & Seq
Forward (+)
14551478 .. 14557397
5920 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21450.t3

Sequence Viewer

Length: 891 bp
ATGCGCTACATTGGTCACCTAGTCATGTCTACAGCTCATCTTCTTGCCTCTGCTTCGATGGATCACACAATCTGCATATGGAATGTGTGGAACAGTGACCAGAAGGTAGCTCGTGTATTGAATTTTCACAATGCCGCAGTCAAAGATGTAAAATGGTCACGGGATGGATTGTTTGTGCTTTCTTGCGGATACGACTCCTATTCAAGGTTAGTGGATGTTGAAAAGGGGATGGAGACTCAAGCTTTTAAGGAGGATCAAGTTGTCAGTGTTGTTAAGTTCAATCCTGAAAATTCAAAACTCTTCATTGCTGGGGGATCAAGAGGCAGCCTCAAGCTGTGGGATGTTAGAAATGGCAAGGTGGTCCATGACTACATTCGAGGTCATGATCCTATCCTTGATGTCGAATTCACCAAGAATGGCAAGCTGATTATTTCCTCAAGTGATGTATCTGGACGAAACCTTAGTGAGAATTCTATTGTTGTTTGGGATGTTTCACGAGAAGTTCCTCTCTCTAATCAGGTGTACGTAGAAGCATTCACCTGTCCCTGTGTTAGATTCCACCCTTTCGAACAATCGTTTGTTGCCCAGTCAAATGGTAATTATATCGCAATCTTCTCTGCAAGCCCTCCCTTCAAGATGAACAAGTACAAGAGGTACGAAGGCCATGGGGTATCAGGGTTTCCAATAAAATGTGTGTTTAGCTTGGACGGGGAAAAGCTACTGTCAGGCTCCTCAGAGGGTTCAGTATACTTCTATGATTATGGGACTTCTGAGGTTATCAAGAAAATCAAGGCATTTGAGCAGGCCAGCATAGATATTGCTGTCCACCCAATCATACCTAATGTGATTGCTGCTTGCAGTTGGAATGGAGAAGTTTCTGTGTTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

297

Amino Acids

33.06

Weight (kDa)

6.51

Isoelectric Point (pI)

27.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 130 - 263 2.5e-12 CAF1B/HIR1 beta-propeller domain
WD40_Prp19 PF24814 130 - 332 2.9e-20 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 130 - 221 7.6e-12 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 131 - 171 1.1e-07 THOC3 beta-propeller domain
Beta-prop_EML PF23409 132 - 285 1.3e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_TEP1_2nd PF25047 132 - 254 2.3e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 133 - 175 8.1e-06 CDC20/Fizzy WD40 domain
WD40 PF00400 134 - 167 3.1e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 134 - 433 1.4e-43 WDR5 beta-propeller domain
EIF3I PF24805 134 - 244 2.6e-07 EIF3I
Beta-prop_TEP1_2nd PF25047 134 - 267 5.9e-07 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 135 - 304 2.6e-16 CDC20/Fizzy WD40 domain
Beta-prop_Aladin PF25460 137 - 238 2.3e-06 Aladin seven-bladed propeller
WDR55 PF24796 139 - 287 1e-17 WDR55
Beta-prop_WDR36-Utp21_2nd PF25168 150 - 240 3.9e-07 WDR36/Utp21 second beta-propeller domain
WD40_Gbeta PF25391 172 - 289 2.1e-08 G protein beta WD-40 repeat protein
Beta-prop_THOC3 PF25174 182 - 407 5.6e-22 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 209 - 286 4.8e-06 WDR36/Utp21 first beta-propeller
Beta-prop_VPS8 PF23410 218 - 290 8.3e-06 Vps8 beta-propeller
WD40_WDHD1_1st PF24817 266 - 432 1.7e-07 WDHD1 first WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 29, 747
AciI CCGC 2 cut(s) 135, 186
AclWI GGATC 4 cut(s) 69, 261, 322, 380
AcsI RAATTY 4 cut(s) 121, 289, 404, 469
AfaI GTAC 3 cut(s) 524, 647, 656
AfiI CCNNNNNNNGG 1 cut(s) 204
AgsI TTSAA 6 cut(s) 121, 204, 221, 280, 294, 634
AluBI AGCT 7 cut(s) 35, 110, 242, 334, 424, 702, 718
AluI AGCT 7 cut(s) 35, 110, 242, 334, 424, 702, 718
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 4 cut(s) 69, 261, 322, 380
AoxI GGCC 2 cut(s) 661, 804
ApeKI GCWGC 2 cut(s) 324, 851
ApoI RAATTY 4 cut(s) 121, 289, 404, 469
AspLEI GCGC 1 cut(s) 6
AspS9I GGNCC 1 cut(s) 361
AsuHPI GGTGA 3 cut(s) 8, 400, 529
AsuII TTCGAA 1 cut(s) 567
AvaII GGWCC 1 cut(s) 361
BauI CACGAG 2 cut(s) 111, 495
BbvI GCAGC 2 cut(s) 336, 838
BccI CCATC 3 cut(s) 52, 158, 223
BciVI GTATCC 1 cut(s) 182
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 30
BfuI GTATCC 1 cut(s) 182
BisI GCNGC 3 cut(s) 135, 325, 852
BlsI GCNGC 3 cut(s) 136, 326, 853
Bme18I GGWCC 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 730
BmrI ACTGGG 1 cut(s) 580
BmuI ACTGGG 1 cut(s) 580
BplI GAGNNNNNCTC 2 cut(s) 312, 344
Bpu14I TTCGAA 1 cut(s) 567
BpuEI CTTGAG 3 cut(s) 222, 314, 421
BsaAI YACGTR 1 cut(s) 526
BsaJI CCNNGG 1 cut(s) 664
Bsc4I CCNNNNNNNGG 1 cut(s) 204
Bse1I ACTGG 1 cut(s) 586
Bse3DI GCAATG 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 664
BseGI GGATG 5 cut(s) 169, 220, 234, 346, 493
BseLI CCNNNNNNNGG 1 cut(s) 204
BseMI GCAATG 1 cut(s) 303
BseMII CTCAG 2 cut(s) 747, 762
BseNI ACTGG 1 cut(s) 586
BseRI GAGGAG 1 cut(s) 721
BseXI GCAGC 2 cut(s) 336, 838
BseYI CCCAGC 1 cut(s) 308
BshFI GGCC 2 cut(s) 663, 806
BslFI GGGAC 2 cut(s) 528, 778
BslI CCNNNNNNNGG 1 cut(s) 204
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 2 cut(s) 528, 778
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 2 cut(s) 663, 806
Bsp119I TTCGAA 1 cut(s) 567
Bsp143I GATC 4 cut(s) 61, 253, 314, 385
Bsp19I CCATGG 1 cut(s) 664
BspACI CCGC 2 cut(s) 135, 186
BspANI GGCC 2 cut(s) 663, 806
BspCNI CTCAG 2 cut(s) 746, 763
BspHI TCATGA 1 cut(s) 382
BspLI GGNNCC 1 cut(s) 730
BspPI GGATC 4 cut(s) 69, 261, 322, 380
BspT104I TTCGAA 1 cut(s) 567
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 1 cut(s) 586
BssECI CCNNGG 1 cut(s) 664
BssMI GATC 4 cut(s) 61, 253, 314, 385
BssNAI GTATAC 1 cut(s) 748
BssSI CACGAG 2 cut(s) 111, 495
BssT1I CCWWGG 1 cut(s) 664
Bst1107I GTATAC 1 cut(s) 748
Bst2BI CACGAG 2 cut(s) 111, 495
Bst4CI ACNGT 2 cut(s) 95, 723
Bst6I CTCTTC 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 567
BstC8I GCNNGC 5 cut(s) 422, 622, 804, 808, 856
BstDEI CTNAG 3 cut(s) 461, 733, 771
BstDSI CCRYGG 1 cut(s) 664
BstEII GGTNACC 1 cut(s) 14
BstENI CCTNNNNNAGG 1 cut(s) 202
BstF5I GGATG 5 cut(s) 169, 220, 234, 346, 493
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 4 cut(s) 64, 256, 317, 388
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 61, 253, 314, 385
BstPI GGTNACC 1 cut(s) 14
BstSFI CTRYAG 1 cut(s) 30
BstSNI TACGTA 1 cut(s) 526
BstV1I GCAGC 2 cut(s) 336, 838
BstXI CCANNNNNNTGG 1 cut(s) 593
BstZ17I GTATAC 1 cut(s) 748
BsuI GTATCC 1 cut(s) 182
BsuRI GGCC 2 cut(s) 663, 806
BtgI CCRYGG 1 cut(s) 664
BtsCI GGATG 5 cut(s) 169, 220, 234, 346, 493
BtsIMutI CAGTG 2 cut(s) 100, 271
Cac8I GCNNGC 5 cut(s) 422, 622, 804, 808, 856
CciI TCATGA 1 cut(s) 382
CfoI GCGC 1 cut(s) 6
Cfr13I GGNCC 1 cut(s) 361
Csp6I GTAC 3 cut(s) 523, 646, 655
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 4 cut(s) 25, 365, 383, 665
CviQI GTAC 3 cut(s) 523, 646, 655
DdeI CTNAG 3 cut(s) 461, 733, 771
DpnI GATC 4 cut(s) 63, 255, 316, 387
DpnII GATC 4 cut(s) 61, 253, 314, 385
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
Eco105I TACGTA 1 cut(s) 526
Eco130I CCWWGG 1 cut(s) 664
Eco47I GGWCC 1 cut(s) 361
Eco91I GGTNACC 1 cut(s) 14
EcoNI CCTNNNNNAGG 1 cut(s) 202
EcoO65I GGTNACC 1 cut(s) 14
EcoRI GAATTC 2 cut(s) 404, 469
EcoT14I CCWWGG 1 cut(s) 664
ErhI CCWWGG 1 cut(s) 664
FaeI CATG 4 cut(s) 28, 368, 386, 668
FaqI GGGAC 2 cut(s) 528, 778
FatI CATG 4 cut(s) 24, 364, 382, 664
FauNDI CATATG 1 cut(s) 77
FblI GTMKAC 2 cut(s) 29, 747
Fnu4HI GCNGC 3 cut(s) 135, 325, 852
FokI GGATG 5 cut(s) 176, 227, 241, 353, 500
Fsp4HI GCNGC 3 cut(s) 135, 325, 852
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 5
GluI GCNGC 3 cut(s) 135, 325, 852
GsaI CCCAGC 1 cut(s) 312
HaeIII GGCC 2 cut(s) 663, 806
HhaI GCGC 1 cut(s) 6
Hin1II CATG 4 cut(s) 28, 368, 386, 668
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HindIII AAGCTT 1 cut(s) 240
HinfI GANTC 3 cut(s) 194, 235, 555
HphI GGTGA 3 cut(s) 8, 400, 529
Hpy166II GTNNAC 4 cut(s) 30, 523, 748, 826
Hpy188I TCNGA 2 cut(s) 736, 772
Hpy188III TCNNGA 7 cut(s) 284, 318, 383, 450, 495, 634, 781
Hpy8I GTNNAC 4 cut(s) 30, 523, 748, 826
HpyAV CCTTC 3 cut(s) 97, 640, 653
HpyCH4III ACNGT 2 cut(s) 95, 723
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 3 cut(s) 75, 620, 858
HpyF3I CTNAG 3 cut(s) 461, 733, 771
HpySE526I ACGT 1 cut(s) 525
Hsp92II CATG 4 cut(s) 28, 368, 386, 668
HspAI GCGC 1 cut(s) 4
Kzo9I GATC 4 cut(s) 61, 253, 314, 385
LmnI GCTCC 1 cut(s) 734
Lsp1109I GCAGC 2 cut(s) 336, 838
MaeI CTAG 1 cut(s) 20
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 3 cut(s) 14, 95, 156
MalI GATC 4 cut(s) 63, 255, 316, 387
MboI GATC 4 cut(s) 61, 253, 314, 385
MboII GAAGA 3 cut(s) 32, 292, 604
MluCI AATT 5 cut(s) 121, 289, 404, 469, 598
MlyI GAGTC 2 cut(s) 188, 229
MmeI TCCRAC 1 cut(s) 842
MseI TTAA 2 cut(s) 246, 273
Mva1269I GAATGC 1 cut(s) 533
NcoI CCATGG 1 cut(s) 664
NdeI CATATG 1 cut(s) 77
NdeII GATC 4 cut(s) 61, 253, 314, 385
NlaIII CATG 4 cut(s) 28, 368, 386, 668
NlaIV GGNNCC 1 cut(s) 730
NmuCI GTSAC 3 cut(s) 14, 95, 156
NspV TTCGAA 1 cut(s) 567
PagI TCATGA 1 cut(s) 382
PctI GAATGC 1 cut(s) 533
PfeI GAWTC 1 cut(s) 555
PkrI GCNGC 3 cut(s) 136, 326, 853
PleI GAGTC 2 cut(s) 188, 229
PpsI GAGTC 2 cut(s) 188, 229
Ppu21I YACGTR 1 cut(s) 526
PspEI GGTNACC 1 cut(s) 14
PspFI CCCAGC 1 cut(s) 308
PspN4I GGNNCC 1 cut(s) 730
PspPI GGNCC 1 cut(s) 361
RsaI GTAC 3 cut(s) 524, 647, 656
RsaNI GTAC 3 cut(s) 523, 646, 655
SaqAI TTAA 2 cut(s) 246, 273
SatI GCNGC 3 cut(s) 135, 325, 852
Sau3AI GATC 4 cut(s) 61, 253, 314, 385
Sau96I GGNCC 1 cut(s) 361
SchI GAGTC 2 cut(s) 188, 229
SfcI CTRYAG 1 cut(s) 30
SfuI TTCGAA 1 cut(s) 567
SinI GGWCC 1 cut(s) 361
SmlI CTYRAG 3 cut(s) 237, 329, 436
SmoI CTYRAG 3 cut(s) 237, 329, 436
SnaBI TACGTA 1 cut(s) 526
Sse9I AATT 5 cut(s) 121, 289, 404, 469, 598
SsiI CCGC 2 cut(s) 135, 186
SspMI CTAG 1 cut(s) 20
StyI CCWWGG 1 cut(s) 664
TaaI ACNGT 2 cut(s) 95, 723
TaiI ACGT 1 cut(s) 528
TaqI TCGA 4 cut(s) 56, 376, 402, 567
TasI AATT 5 cut(s) 121, 289, 404, 469, 598
TatI WGTACW 1 cut(s) 645
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 555
Tru1I TTAA 2 cut(s) 246, 273
Tru9I TTAA 2 cut(s) 246, 273
TscAI CASTG 2 cut(s) 100, 271
TseFI GTSAC 3 cut(s) 14, 95, 156
TseI GCWGC 2 cut(s) 324, 851
Tsp45I GTSAC 3 cut(s) 14, 95, 156
TspDTI ATGAA 2 cut(s) 292, 653
TspRI CASTG 2 cut(s) 100, 271
VpaK11BI GGWCC 1 cut(s) 361
XagI CCTNNNNNAGG 1 cut(s) 202
XapI RAATTY 4 cut(s) 121, 289, 404, 469
XmiI GTMKAC 2 cut(s) 29, 747
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.