MD11G1230500.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008102
Physical Location & Seq
Reverse (-)
33547997 .. 33549627
1631 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1230500.v1.1.491

Sequence Viewer

Length: 651 bp
ATGGACCTGCTCTGCAACGTATACTCTAATCCCTCGGACAACGAAGAAGAAGAAGAAGAAGAAGAAGCAGAAGGAATTAAGCAAACCATCACACCCCCGAATCCGAAGCGACTCAAATCCGATAACCCATTTGCACATACCAAATTTCACCATCCTCTGCCGGAGTCAACCCAGTTTCCTACCCTCCGAGCAGAAGCTCCGGTTCCTGGTCGATACATATCCAAGAGGGAGAGGGCGTTATTGGCTTCCCAACCTACTGTCCCCGACCCGCAGCCGAACCCGAATGGTCCTGTCACGACCTTGCCTGTTTTTGGGAGTATTTCGGATTCAGACGTTCCTCGAGATGTGATGTTGTTGTTGAGAAACAAAGCGAAAAAGGGTCGATCGCAGCTCGGCCAATTGCCCCAAAGAATGTCTGTAGCTCTAAATAAGCATACCCAAGCTGTCAATGCTGTACATTGGTCACCAACCCACGCTCATCTTCTTGCCTCTGCTTCAATGGATCACAAAATCTGCATATGGAATGTGTGGAGCAGTGATCAGAAGGTAGCCCGTGTGTTCAATTTTCATAACGCTGCGGTCAAAGATGTGAATGGTCACTGCAAGGGTTGTCTGTTCTTTCTTGTGGATATGACTCCTCATCAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

217

Amino Acids

24.08

Weight (kDa)

7.18

Isoelectric Point (pI)

49.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_CAF1B_HIR1 PF24105 133 - 184 1.1e-06 CAF1B/HIR1 beta-propeller domain
Beta-prop_RIG_2nd PF23775 136 - 182 3.9e-07 RIG second beta-propeller
WD40_Prp19 PF24814 137 - 206 6.8e-08 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 137 - 197 1.3e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 139 - 184 9.7e-09 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 140 - 203 1.8e-07 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 140 - 195 3.2e-07 CDC20/Fizzy WD40 domain
WD40 PF00400 142 - 175 3.8e-07 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 21
AciI CCGC 2 cut(s) 269, 578
AclWI GGATC 1 cut(s) 510
AcoI YGGCCR 1 cut(s) 394
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 1 cut(s) 456
AfiI CCNNNNNNNGG 3 cut(s) 206, 311, 644
AgsI TTSAA 2 cut(s) 498, 562
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 4 cut(s) 197, 391, 422, 443
AluI AGCT 4 cut(s) 197, 391, 422, 443
AlwI GGATC 1 cut(s) 510
Ama87I CYCGRG 1 cut(s) 339
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 3 cut(s) 271, 388, 575
ApoI RAATTY 1 cut(s) 143
AspS9I GGNCC 2 cut(s) 4, 287
AsuHPI GGTGA 2 cut(s) 140, 456
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 2 cut(s) 4, 287
BbvI GCAGC 3 cut(s) 283, 400, 562
BccI CCATC 2 cut(s) 95, 159
BciT130I CCWGG 1 cut(s) 207
BclI TGATCA 1 cut(s) 538
BfaI CTAG 1 cut(s) 649
BfmI CTRYAG 1 cut(s) 417
BfuAI ACCTGC 1 cut(s) 15
BisI GCNGC 3 cut(s) 272, 389, 576
BlsI GCNGC 3 cut(s) 273, 390, 577
Bme1390I CCNGG 1 cut(s) 207
Bme18I GGWCC 2 cut(s) 4, 287
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 4, 287
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 207
BmrI ACTGGG 1 cut(s) 166
BmuI ACTGGG 1 cut(s) 166
BsaBI GATNNNNATC 1 cut(s) 217
BsaJI CCNNGG 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 199
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 311, 644
Bse1I ACTGG 1 cut(s) 172
Bse8I GATNNNNATC 1 cut(s) 217
BseBI CCWGG 1 cut(s) 207
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 1 cut(s) 151
BseJI GATNNNNATC 1 cut(s) 217
BseLI CCNNNNNNNGG 3 cut(s) 206, 311, 644
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 627
BseXI GCAGC 3 cut(s) 283, 400, 562
Bsh1285I CGRYCG 1 cut(s) 386
BshFI GGCC 1 cut(s) 396
BsiEI CGRYCG 1 cut(s) 386
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 2 cut(s) 161, 200
BslFI GGGAC 1 cut(s) 245
BslI CCNNNNNNNGG 3 cut(s) 206, 311, 644
BsmFI GGGAC 1 cut(s) 245
BsnI GGCC 1 cut(s) 396
BsoBI CYCGRG 1 cut(s) 339
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 3 cut(s) 383, 502, 538
BspACI CCGC 2 cut(s) 269, 578
BspANI GGCC 1 cut(s) 396
BspLI GGNNCC 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 15
BspPI GGATC 1 cut(s) 510
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 3 cut(s) 383, 502, 538
BssNAI GTATAC 1 cut(s) 22
Bst1107I GTATAC 1 cut(s) 22
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 1 cut(s) 259
BstAUI TGTACA 1 cut(s) 454
BstEII GGTNACC 1 cut(s) 462
BstENI CCTNNNNNAGG 1 cut(s) 642
BstF5I GGATG 1 cut(s) 151
BstKTI GATC 3 cut(s) 386, 505, 541
BstMBI GATC 3 cut(s) 383, 502, 538
BstMCI CGRYCG 1 cut(s) 386
BstMWI GCNNNNNNNGC 2 cut(s) 242, 449
BstNI CCWGG 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 462
BstSCI CCNGG 1 cut(s) 205
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 3 cut(s) 283, 400, 562
BstZ17I GTATAC 1 cut(s) 22
BsuRI GGCC 1 cut(s) 396
BtsCI GGATG 1 cut(s) 151
BtsI GCAGTG 2 cut(s) 541, 598
BtsIMutI CAGTG 2 cut(s) 541, 598
BveI ACCTGC 1 cut(s) 15
Cfr13I GGNCC 2 cut(s) 4, 287
Csp6I GTAC 1 cut(s) 455
CviJI RGCY 9 cut(s) 197, 245, 274, 391, 396, 422, 443, 551, 648
CviKI_1 RGCY 9 cut(s) 197, 245, 274, 391, 396, 422, 443, 551, 648
CviQI GTAC 1 cut(s) 455
DpnI GATC 3 cut(s) 385, 504, 540
DpnII GATC 3 cut(s) 383, 502, 538
EaeI YGGCCR 1 cut(s) 394
Eco47I GGWCC 2 cut(s) 4, 287
Eco88I CYCGRG 1 cut(s) 339
Eco91I GGTNACC 1 cut(s) 462
EcoNI CCTNNNNNAGG 1 cut(s) 642
EcoO65I GGTNACC 1 cut(s) 462
EcoRII CCWGG 1 cut(s) 205
FaiI YATR 8 cut(s) 22, 138, 218, 435, 518, 520, 570, 632
FaqI GGGAC 1 cut(s) 245
FauI CCCGC 1 cut(s) 276
FauNDI CATATG 1 cut(s) 518
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 3 cut(s) 272, 389, 576
FokI GGATG 1 cut(s) 138
Fsp4HI GCNGC 3 cut(s) 272, 389, 576
FspBI CTAG 1 cut(s) 649
GluI GCNGC 3 cut(s) 272, 389, 576
HaeIII GGCC 1 cut(s) 396
HapII CCGG 2 cut(s) 161, 200
HincII GTYRAC 1 cut(s) 168
HindII GTYRAC 1 cut(s) 168
HinfI GANTC 5 cut(s) 100, 111, 164, 326, 634
HpaII CCGG 2 cut(s) 161, 200
HphI GGTGA 2 cut(s) 140, 456
Hpy166II GTNNAC 2 cut(s) 22, 168
Hpy188I TCNGA 7 cut(s) 37, 105, 121, 188, 325, 331, 543
Hpy188III TCNNGA 2 cut(s) 295, 341
Hpy8I GTNNAC 2 cut(s) 22, 168
HpyAV CCTTC 2 cut(s) 65, 538
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 2 cut(s) 18, 333
HpyCH4V TGCA 4 cut(s) 15, 134, 516, 603
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 449
HpySE526I ACGT 2 cut(s) 18, 333
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 3 cut(s) 383, 502, 538
LmnI GCTCC 2 cut(s) 202, 531
LpnPI CCDG 8 cut(s) 20, 174, 185, 192, 213, 219, 303, 318
Lsp1109I GCAGC 3 cut(s) 283, 400, 562
MaeI CTAG 1 cut(s) 649
MaeII ACGT 2 cut(s) 18, 333
MaeIII GTNAC 3 cut(s) 292, 462, 596
MalI GATC 3 cut(s) 385, 504, 540
MboI GATC 3 cut(s) 383, 502, 538
MboII GAAGA 8 cut(s) 56, 59, 62, 65, 68, 71, 74, 473
MfeI CAATTG 1 cut(s) 398
MluCI AATT 4 cut(s) 75, 143, 398, 562
MlyI GAGTC 3 cut(s) 105, 173, 628
MnlI CCTC 8 cut(s) 43, 165, 194, 219, 225, 348, 499, 648
MseI TTAA 1 cut(s) 78
MspI CCGG 2 cut(s) 161, 200
MspR9I CCNGG 1 cut(s) 207
MunI CAATTG 1 cut(s) 398
MvaI CCWGG 1 cut(s) 207
MwoI GCNNNNNNNGC 2 cut(s) 242, 449
NdeI CATATG 1 cut(s) 518
NdeII GATC 3 cut(s) 383, 502, 538
NlaIV GGNNCC 1 cut(s) 204
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 3 cut(s) 292, 462, 596
PaeR7I CTCGAG 1 cut(s) 339
PfeI GAWTC 2 cut(s) 100, 326
PkrI GCNGC 3 cut(s) 273, 390, 577
Ple19I CGATCG 1 cut(s) 386
PleI GAGTC 3 cut(s) 105, 172, 628
PpsI GAGTC 3 cut(s) 105, 172, 628
Psp6I CCWGG 1 cut(s) 205
PspEI GGTNACC 1 cut(s) 462
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 2 cut(s) 4, 287
PvuI CGATCG 1 cut(s) 386
RsaI GTAC 1 cut(s) 456
RsaNI GTAC 1 cut(s) 455
SaqAI TTAA 1 cut(s) 78
SatI GCNGC 3 cut(s) 272, 389, 576
Sau3AI GATC 3 cut(s) 383, 502, 538
Sau96I GGNCC 2 cut(s) 4, 287
SchI GAGTC 3 cut(s) 105, 173, 628
ScrFI CCNGG 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 417
Sfr274I CTCGAG 1 cut(s) 339
SinI GGWCC 2 cut(s) 4, 287
SlaI CTCGAG 1 cut(s) 339
SmlI CTYRAG 1 cut(s) 339
SmoI CTYRAG 1 cut(s) 339
Sse9I AATT 4 cut(s) 75, 143, 398, 562
SsiI CCGC 2 cut(s) 269, 578
SspMI CTAG 1 cut(s) 649
StyD4I CCNGG 1 cut(s) 205
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 2 cut(s) 21, 336
TaqI TCGA 3 cut(s) 211, 340, 382
TasI AATT 4 cut(s) 75, 143, 398, 562
TatI WGTACW 1 cut(s) 454
TfiI GAWTC 2 cut(s) 100, 326
Tru1I TTAA 1 cut(s) 78
Tru9I TTAA 1 cut(s) 78
TscAI CASTG 2 cut(s) 541, 605
TseFI GTSAC 3 cut(s) 292, 462, 596
TseI GCWGC 3 cut(s) 271, 388, 575
Tsp45I GTSAC 3 cut(s) 292, 462, 596
TspDTI ATGAA 1 cut(s) 557
TspRI CASTG 2 cut(s) 541, 605
VpaK11BI GGWCC 2 cut(s) 4, 287
XagI CCTNNNNNAGG 1 cut(s) 642
XapI RAATTY 1 cut(s) 143
XhoI CTCGAG 1 cut(s) 339
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 1 cut(s) 649
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.