MD03G1215900.v1.1

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008102
Physical Location & Seq
Reverse (-)
29687770 .. 29699045
11276 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1215900.v1.1.491

Sequence Viewer

Length: 1320 bp
ATGGATCTGCTCTGCAACGCATACTGTAATGCCTCCGACAACGAAGAAGCAGAAGCAGAAGGAATTAAAGAAACCGTCACACCCCCCAACCCGAAGCGACTCAAACCCGATAACCCGTTTGCACATACCAAATTTCACCATCCTCTACCGGAGTCAACCCAGTTTCCGACCACCCAAGCAGAAGCTCCGGTTCCTGGTCGATACATATCCAAGAGGGAGAGGGCGTTATTGGCTACCCAACCTACTGTCCCCGAACCGAACCCGAACCCGAACGCTCCTGTCACGACCTTGCCTGTTTTTGGGAGTATTTCGGATTCAGACGTTCGTCGAGATGTTATGTCGTTGTTGAGAAACAAAGGGAAAAAGGGTCCATCGCAGCTTGGCCAATTGCCCCAAAGAACGTCTGTAGCTCTAAATAGGCATACCAAAGCTGTCAATGCTGTGCATTGGTCACCAACTCATGCTCATCTTCTTGCCTCTGCTTCAATGGATCACACAATCTGCATATGGAATGTGTGGAGCAGTGATGAGAAGGTAGCCCGTGTGTTGAATTTTCACAGTGCTGCAGTCAAAGATGTGAAATGGTCACAGCAAGGATTGTCTGTTCTTTCTTGTGGATATGACTCCTCATCAAGGCTAGTAGATGTTGAAAAGGGGTTAGAGATTCAGGTTTTTAAGGAGGATCAAGTTGTCAGTGTTATTAAGTTCCATCCTGACAATTCCAACCTCTTCATATCGGGGGGCTCAAAAGGCGGAATCAGGTTGTGGGATGTTAGAAGTGGCAAGGTGGTCCATGAATATATTCGTGGTCATGATCCCATCCTTGATTTAGAATTCAGCACCAATAGCAAGCAGATTATATCCTCTAGTGATGTATCCGGACGCAACCTCAGTGAAAATTCTATTGTTGTTTGGGATGTTTCACGACAAGTTCCACTATCTAATCAGGTTTATGTGGAAGCCTTTACCTGTCCCTGTGTGAGATCCCACCCATTTGAACCATTTTTTGTTGCCCAGTCAAATGGTAATTATATTGCAATCTTCTCTTTAAGTCCCCCCTTCAAGCTGAACAAGTACAAGAGGTACGAAAGCCATGGAGTATCTGGGTTCCCCATTAAATGTGTATTTAGCTTGGATGGGGAAAAGCTAGTCTCGGGTTCCTCCGATGGTTCTTTATACTTTTATGACTACAGGTCTTCTGAGCTTGTTAGAAAAATCAAGGCGTATGAGCAGGCCTGCATAGACGTTGCTATCCACCCCGTCATGCCGAATGTGATTGCTTCGTGCAGTTGGAACGGAGAGGTTTCAGTGTTCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

440

Amino Acids

48.67

Weight (kDa)

6.94

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_RIG_2nd PF23775 133 - 177 7.4e-06 RIG second beta-propeller
Beta-prop_WDR3_1st PF25173 133 - 226 7.5e-11 WDR3 first beta-propeller domain
WD40_Prp19 PF24814 133 - 291 3.7e-23 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 134 - 175 2.4e-08 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 134 - 266 1.1e-12 CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz PF24807 136 - 310 1.4e-18 CDC20/Fizzy WD40 domain
EIF3I PF24805 136 - 244 3.1e-07 EIF3I
WD40_MABP1-WDR62_2nd PF24782 136 - 225 2.7e-06 MABP1/WDR62 second WD40 domain
WD40 PF00400 138 - 171 2.1e-07 WD domain, G-beta repeat
Beta-prop_WDR5 PF25175 138 - 437 9.2e-43 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 140 - 216 9.2e-06 Aladin seven-bladed propeller
Beta-prop_WDR36-Utp21_2nd PF25168 153 - 256 1.4e-07 WDR36/Utp21 second beta-propeller domain
WD40_RFWD3 PF23419 174 - 260 5.6e-06 E3 ubiquitin-protein ligase RFWD3 WD40 domain
WD40_Gbeta PF25391 178 - 293 3.2e-06 G protein beta WD-40 repeat protein
WDR55 PF24796 180 - 291 1.3e-15 WDR55
Beta-prop_THOC3 PF25174 186 - 350 1.6e-16 THOC3 beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 205 - 290 3.6e-07 WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st PF25173 230 - 310 2.8e-06 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 252 - 415 1.3e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3 PF25174 376 - 438 3.2e-06 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 878
AciI CCGC 1 cut(s) 753
AclWI GGATC 5 cut(s) 12, 498, 690, 809, 978
AcoI YGGCCR 1 cut(s) 382
AcsI RAATTY 4 cut(s) 131, 550, 833, 898
AfaI GTAC 2 cut(s) 1076, 1085
AfiI CCNNNNNNNGG 3 cut(s) 194, 299, 633
AgsI TTSAA 5 cut(s) 486, 550, 650, 998, 1063
AhdI GACNNNNNGTC 1 cut(s) 1192
AjnI CCWGG 1 cut(s) 193
AloI GAACNNNNNNTCC 2 cut(s) 588, 620
AluBI AGCT 8 cut(s) 185, 379, 410, 431, 1066, 1131, 1147, 1204
AluI AGCT 8 cut(s) 185, 379, 410, 431, 1066, 1131, 1147, 1204
Alw26I GTCTC 1 cut(s) 1156
AlwI GGATC 5 cut(s) 12, 498, 690, 809, 978
Ama87I CYCGRG 1 cut(s) 1153
Aor13HI TCCGGA 1 cut(s) 878
AoxI GGCC 2 cut(s) 382, 1233
ApeKI GCWGC 2 cut(s) 376, 563
ApoI RAATTY 4 cut(s) 131, 550, 833, 898
Asp700I GAANNNNTTC 1 cut(s) 801
AspS9I GGNCC 2 cut(s) 368, 790
AsuHPI GGTGA 2 cut(s) 128, 444
AvaI CYCGRG 1 cut(s) 1153
AvaII GGWCC 2 cut(s) 368, 790
BalI TGGCCA 1 cut(s) 384
BanII GRGCYC 1 cut(s) 746
BbsI GAAGAC 1 cut(s) 1188
BbvI GCAGC 2 cut(s) 388, 550
BccI CCATC 6 cut(s) 147, 379, 717, 827, 1130, 1160
BciT130I CCWGG 1 cut(s) 195
BciVI GTATCC 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 1156
BfaI CTAG 3 cut(s) 638, 867, 1148
BfmI CTRYAG 3 cut(s) 405, 564, 1189
BfuI GTATCC 1 cut(s) 886
BisI GCNGC 2 cut(s) 377, 564
BlsI GCNGC 2 cut(s) 378, 565
Bme1390I CCNGG 1 cut(s) 195
Bme18I GGWCC 2 cut(s) 368, 790
BmeRI GACNNNNNGTC 1 cut(s) 1192
BmeT110I CYCGRG 1 cut(s) 1153
BmgT120I GGNCC 2 cut(s) 368, 790
BmiI GGNNCC 4 cut(s) 192, 369, 1109, 1159
BmrFI CCNGG 1 cut(s) 195
BmrI ACTGGG 2 cut(s) 154, 1009
BmuI ACTGGG 2 cut(s) 154, 1009
BoxI GACNNNNGTC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 1188
BsaBI GATNNNNATC 1 cut(s) 205
BsaJI CCNNGG 1 cut(s) 1093
BsaWI WCCGGW 3 cut(s) 148, 187, 878
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 299, 633
Bse1I ACTGG 2 cut(s) 160, 1015
Bse8I GATNNNNATC 1 cut(s) 205
BseAI TCCGGA 1 cut(s) 878
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 1 cut(s) 1093
BseGI GGATG 6 cut(s) 139, 709, 775, 819, 922, 1141
BseJI GATNNNNATC 1 cut(s) 205
BseLI CCNNNNNNNGG 3 cut(s) 194, 299, 633
BseMII CTCAG 2 cut(s) 904, 1191
BseNI ACTGG 2 cut(s) 160, 1015
BseRI GAGGAG 1 cut(s) 616
BseXI GCAGC 2 cut(s) 388, 550
BsgI GTGCAG 1 cut(s) 1306
BshFI GGCC 2 cut(s) 384, 1235
BsiHKCI CYCGRG 1 cut(s) 1153
BsiSI CCGG 3 cut(s) 149, 188, 879
BslFI GGGAC 3 cut(s) 233, 957, 1038
BslI CCNNNNNNNGG 3 cut(s) 194, 299, 633
BsmAI GTCTC 1 cut(s) 1156
BsmFI GGGAC 3 cut(s) 233, 957, 1038
BsnI GGCC 2 cut(s) 384, 1235
BsoBI CYCGRG 1 cut(s) 1153
Bsp1286I GDGCHC 1 cut(s) 746
Bsp13I TCCGGA 1 cut(s) 878
Bsp143I GATC 5 cut(s) 4, 490, 682, 814, 983
Bsp19I CCATGG 1 cut(s) 1093
BspACI CCGC 1 cut(s) 753
BspANI GGCC 2 cut(s) 384, 1235
BspCNI CTCAG 2 cut(s) 903, 1192
BspEI TCCGGA 1 cut(s) 878
BspHI TCATGA 1 cut(s) 811
BspLI GGNNCC 4 cut(s) 192, 369, 1109, 1159
BspMAI CTGCAG 1 cut(s) 568
BspPI GGATC 5 cut(s) 12, 498, 690, 809, 978
BsrI ACTGG 2 cut(s) 160, 1015
BssECI CCNNGG 1 cut(s) 1093
BssMI GATC 5 cut(s) 4, 490, 682, 814, 983
BssT1I CCWWGG 1 cut(s) 1093
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 4 cut(s) 26, 76, 247, 560
Bst6I CTCTTC 1 cut(s) 734
BstC8I GCNNGC 3 cut(s) 851, 1233, 1237
BstDEI CTNAG 2 cut(s) 890, 1200
BstDSI CCRYGG 1 cut(s) 1093
BstEII GGTNACC 1 cut(s) 450
BstENI CCTNNNNNAGG 1 cut(s) 631
BstF5I GGATG 6 cut(s) 139, 709, 775, 819, 922, 1141
BstKTI GATC 5 cut(s) 7, 493, 685, 817, 986
BstMAI GTCTC 1 cut(s) 1156
BstMBI GATC 5 cut(s) 4, 490, 682, 814, 983
BstMWI GCNNNNNNNGC 4 cut(s) 230, 437, 750, 846
BstNI CCWGG 1 cut(s) 195
BstPAI GACNNNNGTC 1 cut(s) 324
BstPI GGTNACC 1 cut(s) 450
BstSCI CCNGG 1 cut(s) 193
BstSFI CTRYAG 3 cut(s) 405, 564, 1189
BstV1I GCAGC 2 cut(s) 388, 550
BstV2I GAAGAC 1 cut(s) 1188
BstX2I RGATCY 2 cut(s) 4, 983
BstXI CCANNNNNNTGG 1 cut(s) 1022
BstYI RGATCY 2 cut(s) 4, 983
BsuI GTATCC 1 cut(s) 886
BsuRI GGCC 2 cut(s) 384, 1235
BtgI CCRYGG 1 cut(s) 1093
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 6 cut(s) 139, 709, 775, 819, 922, 1141
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 5 cut(s) 529, 565, 700, 898, 1314
Cac8I GCNNGC 3 cut(s) 851, 1233, 1237
CciI TCATGA 1 cut(s) 811
Cfr13I GGNCC 2 cut(s) 368, 790
CseI GACGC 1 cut(s) 891
Csp6I GTAC 2 cut(s) 1075, 1084
CviAII CATG 5 cut(s) 461, 794, 812, 1094, 1264
CviQI GTAC 2 cut(s) 1075, 1084
DdeI CTNAG 2 cut(s) 890, 1200
DpnI GATC 5 cut(s) 6, 492, 684, 816, 985
DpnII GATC 5 cut(s) 4, 490, 682, 814, 983
DriI GACNNNNNGTC 1 cut(s) 1192
EaeI YGGCCR 1 cut(s) 382
Eam1104I CTCTTC 1 cut(s) 734
Eam1105I GACNNNNNGTC 1 cut(s) 1192
EarI CTCTTC 1 cut(s) 734
EciI GGCGGA 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 1093
Eco147I AGGCCT 1 cut(s) 1235
Eco24I GRGCYC 1 cut(s) 746
Eco47I GGWCC 2 cut(s) 368, 790
Eco88I CYCGRG 1 cut(s) 1153
Eco91I GGTNACC 1 cut(s) 450
EcoNI CCTNNNNNAGG 1 cut(s) 631
EcoO65I GGTNACC 1 cut(s) 450
EcoRI GAATTC 1 cut(s) 833
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 1093
EcoT38I GRGCYC 1 cut(s) 746
ErhI CCWWGG 1 cut(s) 1093
FaeI CATG 5 cut(s) 464, 797, 815, 1097, 1267
FaqI GGGAC 3 cut(s) 233, 957, 1038
FatI CATG 5 cut(s) 460, 793, 811, 1093, 1263
FauNDI CATATG 1 cut(s) 506
Fnu4HI GCNGC 2 cut(s) 377, 564
FokI GGATG 6 cut(s) 126, 696, 782, 806, 929, 1148
FriOI GRGCYC 1 cut(s) 746
Fsp4HI GCNGC 2 cut(s) 377, 564
FspBI CTAG 3 cut(s) 638, 867, 1148
GluI GCNGC 2 cut(s) 377, 564
HaeIII GGCC 2 cut(s) 384, 1235
HapII CCGG 3 cut(s) 149, 188, 879
HgaI GACGC 1 cut(s) 891
Hin1II CATG 5 cut(s) 464, 797, 815, 1097, 1267
HincII GTYRAC 1 cut(s) 156
HindII GTYRAC 1 cut(s) 156
HinfI GANTC 6 cut(s) 99, 152, 314, 623, 664, 756
HpaII CCGG 3 cut(s) 149, 188, 879
HphI GGTGA 2 cut(s) 128, 444
Hpy166II GTNNAC 1 cut(s) 156
Hpy188I TCNGA 6 cut(s) 37, 168, 313, 319, 1165, 1201
Hpy188III TCNNGA 6 cut(s) 283, 329, 713, 812, 879, 924
Hpy8I GTNNAC 1 cut(s) 156
Hpy99I CGWCG 1 cut(s) 330
HpyAV CCTTC 3 cut(s) 53, 526, 1069
HpyCH4III ACNGT 4 cut(s) 26, 76, 247, 560
HpyCH4IV ACGT 3 cut(s) 321, 401, 1245
HpyCH4V TGCA 8 cut(s) 15, 122, 445, 504, 566, 1037, 1239, 1287
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 437, 750, 846
HpyF3I CTNAG 2 cut(s) 890, 1200
HpySE526I ACGT 3 cut(s) 321, 401, 1245
Hsp92II CATG 5 cut(s) 464, 797, 815, 1097, 1267
Kpn2I TCCGGA 1 cut(s) 878
Kzo9I GATC 5 cut(s) 4, 490, 682, 814, 983
LmnI GCTCC 3 cut(s) 190, 280, 519
Lsp1109I GCAGC 2 cut(s) 388, 550
MaeI CTAG 3 cut(s) 638, 867, 1148
MaeII ACGT 3 cut(s) 321, 401, 1245
MaeIII GTNAC 4 cut(s) 76, 280, 450, 585
MalI GATC 5 cut(s) 6, 492, 684, 816, 985
MboI GATC 5 cut(s) 4, 490, 682, 814, 983
MboII GAAGA 5 cut(s) 56, 461, 721, 1033, 1188
MfeI CAATTG 1 cut(s) 386
MflI RGATCY 2 cut(s) 4, 983
MhlI GDGCHC 1 cut(s) 746
MlsI TGGCCA 1 cut(s) 384
MluCI AATT 8 cut(s) 63, 131, 386, 550, 718, 833, 898, 1027
MluNI TGGCCA 1 cut(s) 384
MlyI GAGTC 3 cut(s) 93, 161, 617
MmeI TCCRAC 4 cut(s) 60, 191, 747, 1271
Mox20I TGGCCA 1 cut(s) 384
MroI TCCGGA 1 cut(s) 878
MroXI GAANNNNTTC 1 cut(s) 801
MscI TGGCCA 1 cut(s) 384
MseI TTAA 5 cut(s) 66, 675, 702, 1049, 1116
Msp20I TGGCCA 1 cut(s) 384
MspI CCGG 3 cut(s) 149, 188, 879
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 1 cut(s) 386
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 4 cut(s) 230, 437, 750, 846
NcoI CCATGG 1 cut(s) 1093
NdeI CATATG 1 cut(s) 506
NdeII GATC 5 cut(s) 4, 490, 682, 814, 983
NlaIII CATG 5 cut(s) 464, 797, 815, 1097, 1267
NlaIV GGNNCC 4 cut(s) 192, 369, 1109, 1159
NmuCI GTSAC 4 cut(s) 76, 280, 450, 585
PagI TCATGA 1 cut(s) 811
PceI AGGCCT 1 cut(s) 1235
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 3 cut(s) 314, 664, 756
PkrI GCNGC 2 cut(s) 378, 565
PleI GAGTC 3 cut(s) 93, 160, 617
PpsI GAGTC 3 cut(s) 93, 160, 617
PshAI GACNNNNGTC 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 193
PspEI GGTNACC 1 cut(s) 450
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 4 cut(s) 192, 369, 1109, 1159
PspPI GGNCC 2 cut(s) 368, 790
PstI CTGCAG 1 cut(s) 568
PsuI RGATCY 2 cut(s) 4, 983
RsaI GTAC 2 cut(s) 1076, 1085
RsaNI GTAC 2 cut(s) 1075, 1084
SaqAI TTAA 5 cut(s) 66, 675, 702, 1049, 1116
SatI GCNGC 2 cut(s) 377, 564
Sau3AI GATC 5 cut(s) 4, 490, 682, 814, 983
Sau96I GGNCC 2 cut(s) 368, 790
SchI GAGTC 3 cut(s) 93, 161, 617
ScrFI CCNGG 1 cut(s) 195
SduI GDGCHC 1 cut(s) 746
SfcI CTRYAG 3 cut(s) 405, 564, 1189
SinI GGWCC 2 cut(s) 368, 790
Sse9I AATT 8 cut(s) 63, 131, 386, 550, 718, 833, 898, 1027
SseBI AGGCCT 1 cut(s) 1235
SsiI CCGC 1 cut(s) 753
SspMI CTAG 3 cut(s) 638, 867, 1148
StuI AGGCCT 1 cut(s) 1235
StyD4I CCNGG 1 cut(s) 193
StyI CCWWGG 1 cut(s) 1093
TaaI ACNGT 4 cut(s) 26, 76, 247, 560
TaiI ACGT 3 cut(s) 324, 404, 1248
TaqI TCGA 3 cut(s) 199, 328, 1314
TasI AATT 8 cut(s) 63, 131, 386, 550, 718, 833, 898, 1027
TatI WGTACW 1 cut(s) 1074
TfiI GAWTC 3 cut(s) 314, 664, 756
Tru1I TTAA 5 cut(s) 66, 675, 702, 1049, 1116
Tru9I TTAA 5 cut(s) 66, 675, 702, 1049, 1116
TscAI CASTG 5 cut(s) 529, 565, 700, 898, 1314
TseFI GTSAC 4 cut(s) 76, 280, 450, 585
TseI GCWGC 2 cut(s) 376, 563
Tsp45I GTSAC 4 cut(s) 76, 280, 450, 585
TspDTI ATGAA 2 cut(s) 721, 810
TspGWI ACGGA 1 cut(s) 1311
TspRI CASTG 5 cut(s) 529, 565, 700, 898, 1314
VpaK11BI GGWCC 2 cut(s) 368, 790
XagI CCTNNNNNAGG 1 cut(s) 631
XapI RAATTY 4 cut(s) 131, 550, 833, 898
XcmI CCANNNNNNNNNTGG 1 cut(s) 1100
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 3 cut(s) 638, 867, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.