AT5G57030

Lycopene epsilon cyclase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012912
Physical Location & Seq
Forward (+)
23077120 .. 23080096
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57030.1

Sequence Viewer

Length: 1575 bp
ATGGAGTGTGTTGGGGCTAGGAATTTCGCAGCAATGGCGGTTTCAACATTTCCGTCATGGAGTTGTCGAAGGAAATTTCCAGTGGTTAAGAGATACAGCTATAGGAATATTCGTTTCGGTTTGTGTAGTGTCAGAGCTAGCGGCGGCGGAAGTTCCGGTAGTGAGAGTTGTGTAGCGGTGAGAGAAGATTTCGCTGACGAAGAAGATTTTGTGAAAGCTGGTGGTTCTGAGATTCTATTTGTTCAAATGCAGCAGAACAAAGATATGGATGAACAGTCTAAGCTTGTTGATAAGTTGCCTCCTATATCAATTGGTGATGGTGCTTTGGATCTAGTGGTTATTGGTTGTGGTCCTGCTGGTTTAGCCTTGGCTGCAGAATCAGCTAAGCTTGGATTAAAAGTTGGACTCATTGGTCCAGATCTTCCTTTTACTAACAATTACGGTGTTTGGGAAGATGAATTCAATGATCTTGGGCTGCAAAAATGTATTGAGCATGTTTGGAGAGAGACTATTGTGTATCTGGATGATGACAAGCCTATTACCATTGGCCGTGCTTATGGAAGAGTTAGTCGACGTTTGCTCCATGAGGAGCTTTTGAGGAGGTGTGTCGAGTCAGGTGTCTCGTACCTTAGCTCGAAAGTTGACAGCATAACAGAAGCTTCTGATGGCCTTAGACTTGTTGCTTGTGACGACAATAACGTCATTCCCTGCAGGCTTGCCACTGTTGCTTCTGGAGCAGCTTCGGGAAAGCTCTTGCAATACGAAGTTGGTGGACCTAGAGTCTGTGTGCAAACTGCATACGGCGTGGAGGTTGAGGTGGAAAATAGTCCATATGATCCAGATCAAATGGTTTTCATGGATTACAGAGATTATACTAACGAGAAAGTTCGGAGCTTAGAAGCTGAGTATCCAACGTTTCTGTACGCCATGCCTATGACAAAGTCAAGACTCTTCTTCGAGGAGACATGTTTGGCCTCAAAAGATGTCATGCCCTTTGATTTGCTAAAAACGAAGCTCATGTTAAGATTAGATACACTCGGAATTCGAATTCTAAAGACTTACGAAGAGGAGTGGTCCTATATCCCAGTTGGTGGTTCCTTGCCAAACACCGAACAAAAGAATCTCGCCTTTGGTGCTGCCGCTAGCATGGTACATCCCGCAACAGGCTATTCAGTTGTGAGATCTTTGTCTGAAGCTCCAAAATATGCATCAGTCATCGCAGAGATACTAAGAGAAGAGACTACCAAACAGATCAACAGTAATATTTCAAGACAAGCTTGGGATACTTTATGGCCACCAGAAAGGAAAAGACAGAGAGCATTCTTTCTCTTTGGTCTTGCACTCATAGTTCAATTCGATACCGAAGGCATTAGAAGCTTCTTCCGTACTTTCTTCCGCCTTCCAAAATGGATGTGGCAAGGGTTTCTAGGATCAACATTAACATCAGGAGATCTCGTTCTCTTTGCTTTATACATGTTCGTCATTTCACCAAACAATTTGAGAAAAGGTCTCATCAATCATCTCATCTCTGATCCAACCGGAGCAACCATGATAAAAACCTATCTCAAAGTATGA

Protein Analysis

524

Amino Acids

58.49

Weight (kDa)

5.71

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lycopene_cycl PF05834 110 - 501 4e-161 Lycopene cyclase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 411, 698
AccB7I CCANNNNNTGG 1 cut(s) 1091
AccI GTMKAC 1 cut(s) 571
AciI CCGC 8 cut(s) 38, 141, 144, 147, 176, 1140, 1158, 1396
AclI AACGTT 1 cut(s) 914
AclWI GGATC 4 cut(s) 336, 830, 1438, 1526
AcoI YGGCCR 2 cut(s) 547, 1292
AcsI RAATTY 5 cut(s) 22, 74, 458, 1041, 1047
AcuI CTGAAG 1 cut(s) 1212
AfaI GTAC 4 cut(s) 626, 923, 1152, 1387
AfiI CCNNNNNNNGG 2 cut(s) 1091, 1163
AflIII ACRYGT 2 cut(s) 965, 1473
AgsI TTSAA 5 cut(s) 45, 245, 463, 1269, 1352
AhdI GACNNNNNGTC 1 cut(s) 779
AloI GAACNNNNNNTCC 2 cut(s) 1440, 1472
Alw26I GTCTC 5 cut(s) 500, 625, 956, 1232, 1514
AlwI GGATC 4 cut(s) 336, 830, 1438, 1526
AoxI GGCC 4 cut(s) 547, 667, 972, 1292
ApeKI GCWGC 6 cut(s) 29, 250, 371, 475, 737, 1136
ApoI RAATTY 5 cut(s) 22, 74, 458, 1041, 1047
AspS9I GGNCC 4 cut(s) 350, 413, 773, 1074
AsuHPI GGTGA 3 cut(s) 190, 326, 1479
AsuII TTCGAA 1 cut(s) 1045
AsuNHI GCTAGC 2 cut(s) 137, 1142
AvaII GGWCC 4 cut(s) 350, 413, 773, 1074
BalI TGGCCA 1 cut(s) 1294
BbvI GCAGC 6 cut(s) 41, 262, 358, 462, 749, 1123
BccI CCATC 2 cut(s) 311, 659
BceAI ACGGC 2 cut(s) 534, 817
BciVI GTATCC 2 cut(s) 918, 1276
BcoDI GTCTC 5 cut(s) 500, 625, 956, 1232, 1514
BfaI CTAG 6 cut(s) 18, 138, 332, 777, 1143, 1427
BfmI CTRYAG 3 cut(s) 100, 372, 709
BfuI GTATCC 2 cut(s) 918, 1276
BglII AGATCT 3 cut(s) 418, 1181, 1450
BisI GCNGC 9 cut(s) 30, 142, 145, 251, 372, 476, 738, 1137, 1140
BlpI GCTNAGC 1 cut(s) 384
BlsI GCNGC 9 cut(s) 31, 143, 146, 252, 373, 477, 739, 1138, 1141
Bme18I GGWCC 4 cut(s) 350, 413, 773, 1074
BmeRI GACNNNNNGTC 1 cut(s) 779
BmgT120I GGNCC 4 cut(s) 350, 413, 773, 1074
BmiI GGNNCC 1 cut(s) 1096
BmrI ACTGGG 1 cut(s) 1079
BmsI GCATC 1 cut(s) 1217
BmtI GCTAGC 2 cut(s) 141, 1146
BmuI ACTGGG 1 cut(s) 1079
BpmI CTGGAG 1 cut(s) 753
Bpu10I CCTNAGC 1 cut(s) 629
Bpu1102I GCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 1 cut(s) 1045
BsaBI GATNNNNATC 1 cut(s) 840
BsaI GGTCTC 1 cut(s) 1514
BsaJI CCNNGG 1 cut(s) 366
BsaWI WCCGGW 2 cut(s) 155, 1538
BsaXI ACNNNNNCTCC 4 cut(s) 564, 594, 1440, 1470
Bsc4I CCNNNNNNNGG 2 cut(s) 1091, 1163
Bse1I ACTGG 2 cut(s) 80, 1085
Bse3DI GCAATG 1 cut(s) 39
Bse8I GATNNNNATC 1 cut(s) 840
BseDI CCNNGG 1 cut(s) 366
BseGI GGATG 4 cut(s) 274, 529, 1153, 1416
BseJI GATNNNNATC 1 cut(s) 840
BseLI CCNNNNNNNGG 2 cut(s) 1091, 1163
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 219, 894
BseNI ACTGG 2 cut(s) 80, 1085
BseRI GAGGAG 4 cut(s) 602, 613, 974, 1082
BseXI GCAGC 6 cut(s) 41, 262, 358, 462, 749, 1123
BshFI GGCC 4 cut(s) 549, 669, 974, 1294
BsiSI CCGG 2 cut(s) 156, 1539
BslI CCNNNNNNNGG 2 cut(s) 1091, 1163
BsmAI GTCTC 5 cut(s) 500, 625, 956, 1232, 1514
BsmI GAATGC 1 cut(s) 1319
BsnI GGCC 4 cut(s) 549, 669, 974, 1294
Bso31I GGTCTC 1 cut(s) 1514
Bsp119I TTCGAA 1 cut(s) 1045
Bsp1720I GCTNAGC 1 cut(s) 384
BspACI CCGC 8 cut(s) 38, 141, 144, 147, 176, 1140, 1158, 1396
BspANI GGCC 4 cut(s) 549, 669, 974, 1294
BspCNI CTCAG 2 cut(s) 220, 895
BspLI GGNNCC 1 cut(s) 1096
BspMAI CTGCAG 2 cut(s) 376, 713
BspOI GCTAGC 2 cut(s) 141, 1146
BspPI GGATC 4 cut(s) 336, 830, 1438, 1526
BspT104I TTCGAA 1 cut(s) 1045
BspTNI GGTCTC 1 cut(s) 1514
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 2 cut(s) 80, 1085
BssECI CCNNGG 1 cut(s) 366
BssT1I CCWWGG 1 cut(s) 366
Bst4CI ACNGT 4 cut(s) 276, 443, 724, 1259
Bst6I CTCTTC 4 cut(s) 556, 956, 1059, 1230
BstBI TTCGAA 1 cut(s) 1045
BstC8I GCNNGC 4 cut(s) 139, 713, 717, 1144
BstDEI CTNAG 8 cut(s) 228, 279, 384, 629, 671, 895, 903, 1229
BstF5I GGATG 4 cut(s) 274, 529, 1153, 1416
BstMAI GTCTC 5 cut(s) 500, 625, 956, 1232, 1514
BstMWI GCNNNNNNNGC 8 cut(s) 35, 362, 371, 380, 725, 734, 1133, 1374
BstNSI RCATGY 3 cut(s) 497, 969, 1477
BstSFI CTRYAG 3 cut(s) 100, 372, 709
BstV1I GCAGC 6 cut(s) 41, 262, 358, 462, 749, 1123
BstX2I RGATCY 4 cut(s) 328, 418, 1181, 1450
BstYI RGATCY 4 cut(s) 328, 418, 1181, 1450
BsuI GTATCC 2 cut(s) 918, 1276
BsuRI GGCC 4 cut(s) 549, 669, 974, 1294
BtgZI GCGATG 1 cut(s) 1201
BtsCI GGATG 4 cut(s) 274, 529, 1153, 1416
BtsIMutI CAGTG 2 cut(s) 87, 720
Cac8I GCNNGC 4 cut(s) 139, 713, 717, 1144
Cfr13I GGNCC 4 cut(s) 350, 413, 773, 1074
Csp6I GTAC 4 cut(s) 625, 922, 1151, 1386
CviQI GTAC 4 cut(s) 625, 922, 1151, 1386
DdeI CTNAG 8 cut(s) 228, 279, 384, 629, 671, 895, 903, 1229
DrdI GACNNNNNNGTC 2 cut(s) 411, 698
DriI GACNNNNNGTC 1 cut(s) 779
DseDI GACNNNNNNGTC 2 cut(s) 411, 698
EaeI YGGCCR 2 cut(s) 547, 1292
Eam1104I CTCTTC 4 cut(s) 556, 956, 1059, 1230
Eam1105I GACNNNNNGTC 1 cut(s) 779
EarI CTCTTC 4 cut(s) 556, 956, 1059, 1230
EciI GGCGGA 2 cut(s) 162, 1385
Eco130I CCWWGG 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 1514
Eco47I GGWCC 4 cut(s) 350, 413, 773, 1074
Eco57I CTGAAG 1 cut(s) 1212
EcoRI GAATTC 3 cut(s) 458, 1041, 1047
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 1210
ErhI CCWWGG 1 cut(s) 366
FalI AAGNNNNNCTT 2 cut(s) 1261, 1293
FauI CCCGC 1 cut(s) 1165
FauNDI CATATG 1 cut(s) 832
FblI GTMKAC 1 cut(s) 571
Fnu4HI GCNGC 9 cut(s) 30, 142, 145, 251, 372, 476, 738, 1137, 1140
FokI GGATG 4 cut(s) 281, 536, 1140, 1423
Fsp4HI GCNGC 9 cut(s) 30, 142, 145, 251, 372, 476, 738, 1137, 1140
FspBI CTAG 6 cut(s) 18, 138, 332, 777, 1143, 1427
GluI GCNGC 9 cut(s) 30, 142, 145, 251, 372, 476, 738, 1137, 1140
GsuI CTGGAG 1 cut(s) 753
HaeIII GGCC 4 cut(s) 549, 669, 974, 1294
HapII CCGG 2 cut(s) 156, 1539
HincII GTYRAC 2 cut(s) 572, 643
HindII GTYRAC 2 cut(s) 572, 643
HindIII AAGCTT 5 cut(s) 281, 386, 657, 1275, 1375
HinfI GANTC 7 cut(s) 232, 377, 405, 611, 780, 948, 1120
HpaII CCGG 2 cut(s) 156, 1539
HphI GGTGA 3 cut(s) 190, 326, 1479
Hpy166II GTNNAC 3 cut(s) 572, 643, 773
Hpy188I TCNGA 7 cut(s) 134, 229, 664, 891, 1040, 1192, 1531
Hpy188III TCNNGA 8 cut(s) 416, 521, 732, 744, 839, 945, 1269, 1446
Hpy8I GTNNAC 3 cut(s) 572, 643, 773
Hpy99I CGWCG 1 cut(s) 576
HpyAV CCTTC 3 cut(s) 63, 1358, 1409
HpyCH4III ACNGT 4 cut(s) 276, 443, 724, 1259
HpyCH4IV ACGT 3 cut(s) 574, 699, 914
HpyCH4V TGCA 9 cut(s) 250, 374, 478, 711, 757, 790, 797, 1208, 1340
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 362, 371, 380, 725, 734, 1133, 1374
HpyF3I CTNAG 8 cut(s) 228, 279, 384, 629, 671, 895, 903, 1229
HpySE526I ACGT 3 cut(s) 574, 699, 914
LmnI GCTCC 6 cut(s) 585, 589, 734, 891, 1201, 1541
Lsp1109I GCAGC 6 cut(s) 41, 262, 358, 462, 749, 1123
LweI GCATC 1 cut(s) 1217
MaeI CTAG 6 cut(s) 18, 138, 332, 777, 1143, 1427
MaeII ACGT 3 cut(s) 574, 699, 914
MaeIII GTNAC 1 cut(s) 686
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 4 cut(s) 328, 418, 1181, 1450
MlsI TGGCCA 1 cut(s) 1294
MluCI AATT 9 cut(s) 22, 74, 309, 436, 458, 1041, 1047, 1352, 1495
MluNI TGGCCA 1 cut(s) 1294
MlyI GAGTC 4 cut(s) 399, 620, 789, 942
MmeI TCCRAC 3 cut(s) 382, 935, 1559
MnlI CCTC 9 cut(s) 309, 580, 591, 594, 802, 808, 952, 985, 1060
Mox20I TGGCCA 1 cut(s) 1294
Mph1103I ATGCAT 1 cut(s) 1210
MscI TGGCCA 1 cut(s) 1294
MseI TTAA 4 cut(s) 87, 395, 1022, 1439
MslI CAYNNNNRTG 1 cut(s) 932
Msp20I TGGCCA 1 cut(s) 1294
MspI CCGG 2 cut(s) 156, 1539
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 1 cut(s) 1319
MwoI GCNNNNNNNGC 8 cut(s) 35, 362, 371, 380, 725, 734, 1133, 1374
NdeI CATATG 1 cut(s) 832
NheI GCTAGC 2 cut(s) 137, 1142
NlaIV GGNNCC 1 cut(s) 1096
NmuCI GTSAC 1 cut(s) 686
NsiI ATGCAT 1 cut(s) 1210
NspI RCATGY 3 cut(s) 497, 969, 1477
NspV TTCGAA 1 cut(s) 1045
PciI ACATGT 2 cut(s) 965, 1473
PcsI WCGNNNNNNNCGW 1 cut(s) 696
PctI GAATGC 1 cut(s) 1319
PfeI GAWTC 3 cut(s) 232, 377, 1120
PflFI GACNNNGTC 1 cut(s) 940
PflMI CCANNNNNTGG 1 cut(s) 1091
PkrI GCNGC 9 cut(s) 31, 143, 146, 252, 373, 477, 739, 1138, 1141
PleI GAGTC 4 cut(s) 399, 619, 788, 942
PpsI GAGTC 4 cut(s) 399, 619, 788, 942
PscI ACATGT 2 cut(s) 965, 1473
Psp1406I AACGTT 1 cut(s) 914
PspN4I GGNNCC 1 cut(s) 1096
PspPI GGNCC 4 cut(s) 350, 413, 773, 1074
PstI CTGCAG 2 cut(s) 376, 713
PsuI RGATCY 4 cut(s) 328, 418, 1181, 1450
PsyI GACNNNGTC 1 cut(s) 940
RsaI GTAC 4 cut(s) 626, 923, 1152, 1387
RsaNI GTAC 4 cut(s) 625, 922, 1151, 1386
RseI CAYNNNNRTG 1 cut(s) 932
SalI GTCGAC 1 cut(s) 570
SaqAI TTAA 4 cut(s) 87, 395, 1022, 1439
SatI GCNGC 9 cut(s) 30, 142, 145, 251, 372, 476, 738, 1137, 1140
Sau96I GGNCC 4 cut(s) 350, 413, 773, 1074
SbfI CCTGCAGG 1 cut(s) 713
SchI GAGTC 4 cut(s) 399, 620, 789, 942
SdaI CCTGCAGG 1 cut(s) 713
SfaNI GCATC 1 cut(s) 1217
SfcI CTRYAG 3 cut(s) 100, 372, 709
SfuI TTCGAA 1 cut(s) 1045
SinI GGWCC 4 cut(s) 350, 413, 773, 1074
SmiMI CAYNNNNRTG 1 cut(s) 932
Sse8387I CCTGCAGG 1 cut(s) 713
Sse9I AATT 9 cut(s) 22, 74, 309, 436, 458, 1041, 1047, 1352, 1495
SsiI CCGC 8 cut(s) 38, 141, 144, 147, 176, 1140, 1158, 1396
SspI AATATT 2 cut(s) 109, 1264
SspMI CTAG 6 cut(s) 18, 138, 332, 777, 1143, 1427
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 4 cut(s) 276, 443, 724, 1259
TaiI ACGT 3 cut(s) 577, 702, 917
TaqI TCGA 7 cut(s) 67, 571, 609, 635, 957, 1045, 1356
TasI AATT 9 cut(s) 22, 74, 309, 436, 458, 1041, 1047, 1352, 1495
TauI GCSGC 3 cut(s) 144, 147, 1142
TfiI GAWTC 3 cut(s) 232, 377, 1120
Tru1I TTAA 4 cut(s) 87, 395, 1022, 1439
Tru9I TTAA 4 cut(s) 87, 395, 1022, 1439
TscAI CASTG 2 cut(s) 87, 727
TseFI GTSAC 1 cut(s) 686
TseI GCWGC 6 cut(s) 29, 250, 371, 475, 737, 1136
Tsp45I GTSAC 1 cut(s) 686
TspDTI ATGAA 3 cut(s) 285, 471, 844
TspGWI ACGGA 2 cut(s) 42, 1373
TspRI CASTG 2 cut(s) 87, 727
Tth111I GACNNNGTC 1 cut(s) 940
Van91I CCANNNNNTGG 1 cut(s) 1091
VpaK11BI GGWCC 4 cut(s) 350, 413, 773, 1074
XapI RAATTY 5 cut(s) 22, 74, 458, 1041, 1047
XceI RCATGY 3 cut(s) 497, 969, 1477
XcmI CCANNNNNNNNNTGG 1 cut(s) 1410
XmiI GTMKAC 1 cut(s) 571
XspI CTAG 6 cut(s) 18, 138, 332, 777, 1143, 1427
Zsp2I ATGCAT 1 cut(s) 1210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.