Rh2DG085200

Lycopene epsilon cyclase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012912
Physical Location & Seq
Forward (+)
6629088 .. 6631640
2553 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG085200.1

Sequence Viewer

Length: 1128 bp
ATGGACTGCGTTGGAATCGGAGCTCGGAACTTTACGGCAATGACGGTGTCGTTTTGCCCCACTGGGAGAAGAGGGTTTATCCCAAGTACTAGAAAGGAAGCTTCTTTCTTTCGCTACGGTGCTTTGTGTAACAAGCATTCTCTGCTCCAAGTGAGAGCCACTGCCGGAAGTGTGGCTGTCAAGGAAGGCTTTGCGGATGAGGAAGATTACGTGAAGGGCGGTGGGTCGGAGCTTCTTTTCGTCCAAATGCAGCGGAACAAGGCCATGGAGAAACAGTCTAAACTCGTCGATAAGCTTCCGCCAATAAGGGATGATGTACTGGATTTGGTGGTGATTGGTTGTGGTCCGGCGGGTCTTGCTCTGGCTGCCGAGTCGGCTAAGTTGGGATTGAAAGTTGGGCTCATTGGCCCTGACCTGCCTTTCACAAACAACTATGGTGTTTGGGAAGATGAATTCAAAGACCTTGGACTAGAAGGGTGTATTGAGCATGTTTGGCAGGACACCATTGTGTATCTTGATGACGAAAGCGATCCCATTTTGTTCGGTCGTGCTTACGGTCGTGTGTGCCGAAATTTGCTCCATGAGGAGCTTTTGAGAAGGTGTGTAGAGTCAGGTGTTTCATACCTTAGTTCAAGGGTGGAGAGTATTGTTGAAGGTAGCAATGGCCATAGCCTTATAGCATGTGAGCATGGTATCAATGTCCCCTCTAGGCTTGCTACTGTTGCATCAGGAGCTGCTTCAGGGAAGCTTTTGCAATATGAGGTGGGTGGTCCAAAGGTCTCTGTCCAAACAGCTTATGGAGTGGAGGTTGAGGTGGAAAGTTATCCATATGATCCCAACCTGATGGTTTTCATGGATTATAGAGACTACACAAAACAAAAAGTTTCATGTTTGGAAGCAGAATATCCCACATTTCTTTATGCCATGGCCATGTCCCCAACAAGAATATTCTTTGAGGAAACTTGCTTGGCTTCGAAAGATGCCATGCCTTTTGATTTATTGAAGAAAAAGCTCTTGTCAAGGTTAAAGACATTGGGAATTCGTATTGTAAAAACTTATGAAGAGCCTGGAATACCCTCTGGCCACCAGAAAGGAAACGCCAAAGATCATTCTTCCTGTTTGGACTAG

Protein Analysis

375

Amino Acids

41.21

Weight (kDa)

5.65

Isoelectric Point (pI)

35.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lycopene_cycl PF05834 108 - 355 1.3e-86 Lycopene cyclase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 423
AciI CCGC 5 cut(s) 194, 219, 253, 299, 350
AclWI GGATC 2 cut(s) 524, 827
AcoI YGGCCR 3 cut(s) 664, 927, 1081
AcsI RAATTY 3 cut(s) 452, 571, 1038
AcuI CTGAAG 1 cut(s) 723
AfaI GTAC 2 cut(s) 88, 318
AgsI TTSAA 5 cut(s) 391, 457, 633, 653, 1003
AjnI CCWGG 1 cut(s) 1066
AleI CACNNNNGTG 1 cut(s) 506
AluBI AGCT 9 cut(s) 23, 101, 232, 295, 589, 734, 748, 794, 1012
AluI AGCT 9 cut(s) 23, 101, 232, 295, 589, 734, 748, 794, 1012
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 2 cut(s) 784, 858
AlwI GGATC 2 cut(s) 524, 827
AlwNI CAGNNNCTG 1 cut(s) 734
AoxI GGCC 5 cut(s) 261, 406, 664, 927, 1081
ApeKI GCWGC 3 cut(s) 250, 365, 734
ApoI RAATTY 3 cut(s) 452, 571, 1038
AspS9I GGNCC 3 cut(s) 344, 407, 770
AsuHPI GGTGA 1 cut(s) 343
AsuII TTCGAA 1 cut(s) 974
AvaII GGWCC 2 cut(s) 344, 770
BalI TGGCCA 3 cut(s) 666, 929, 1083
BanII GRGCYC 2 cut(s) 25, 402
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 3 cut(s) 262, 352, 721
BccI CCATC 1 cut(s) 838
BceAI ACGGC 1 cut(s) 51
BciT130I CCWGG 1 cut(s) 1068
BcoDI GTCTC 2 cut(s) 784, 858
BfaI CTAG 4 cut(s) 90, 470, 708, 1126
BfuAI ACCTGC 1 cut(s) 423
BglI GCCNNNNNGGC 1 cut(s) 374
BisI GCNGC 3 cut(s) 251, 366, 735
BlsI GCNGC 3 cut(s) 252, 367, 736
BmcAI AGTACT 1 cut(s) 88
Bme1390I CCNGG 1 cut(s) 1068
Bme18I GGWCC 2 cut(s) 344, 770
BmgT120I GGNCC 3 cut(s) 344, 407, 770
BmrFI CCNGG 1 cut(s) 1068
BmrI ACTGGG 1 cut(s) 72
BmsI GCATC 2 cut(s) 734, 970
BmuI ACTGGG 1 cut(s) 72
Bpu14I TTCGAA 1 cut(s) 974
BsaAI YACGTR 1 cut(s) 211
BsaI GGTCTC 1 cut(s) 784
BsaJI CCNNGG 3 cut(s) 264, 463, 924
BsaXI ACNNNNNCTCC 4 cut(s) 260, 290, 792, 822
Bse1I ACTGG 2 cut(s) 67, 324
Bse3DI GCAATG 2 cut(s) 45, 667
BseBI CCWGG 1 cut(s) 1068
BseDI CCNNGG 3 cut(s) 264, 463, 924
BseGI GGATG 2 cut(s) 202, 316
BseMI GCAATG 2 cut(s) 45, 667
BseNI ACTGG 2 cut(s) 67, 324
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 3 cut(s) 262, 352, 721
Bsh1285I CGRYCG 2 cut(s) 547, 559
BshFI GGCC 5 cut(s) 263, 408, 666, 929, 1083
BsiEI CGRYCG 2 cut(s) 547, 559
BsiHKAI GWGCWC 1 cut(s) 25
BsiSI CCGG 2 cut(s) 165, 347
BslFI GGGAC 2 cut(s) 686, 919
BsmAI GTCTC 2 cut(s) 784, 858
BsmFI GGGAC 2 cut(s) 686, 919
BsmI GAATGC 1 cut(s) 136
BsnI GGCC 5 cut(s) 263, 408, 666, 929, 1083
Bso31I GGTCTC 1 cut(s) 784
Bsp119I TTCGAA 1 cut(s) 974
Bsp1286I GDGCHC 2 cut(s) 25, 402
Bsp143I GATC 3 cut(s) 529, 832, 1105
Bsp19I CCATGG 2 cut(s) 264, 924
BspACI CCGC 5 cut(s) 194, 219, 253, 299, 350
BspANI GGCC 5 cut(s) 263, 408, 666, 929, 1083
BspMI ACCTGC 1 cut(s) 423
BspPI GGATC 2 cut(s) 524, 827
BspQI GCTCTTC 1 cut(s) 1056
BspT104I TTCGAA 1 cut(s) 974
BspTNI GGTCTC 1 cut(s) 784
BsrDI GCAATG 2 cut(s) 45, 667
BsrI ACTGG 2 cut(s) 67, 324
BssECI CCNNGG 3 cut(s) 264, 463, 924
BssMI GATC 3 cut(s) 529, 832, 1105
BssT1I CCWWGG 3 cut(s) 264, 463, 924
Bst2UI CCWGG 1 cut(s) 1068
Bst4CI ACNGT 5 cut(s) 46, 119, 276, 557, 721
Bst6I CTCTTC 2 cut(s) 64, 1056
BstAPI GCANNNNNTGC 1 cut(s) 142
BstBAI YACGTR 1 cut(s) 211
BstBI TTCGAA 1 cut(s) 974
BstC8I GCNNGC 1 cut(s) 714
BstDEI CTNAG 2 cut(s) 378, 626
BstDSI CCRYGG 2 cut(s) 264, 924
BstF5I GGATG 2 cut(s) 202, 316
BstKTI GATC 3 cut(s) 532, 835, 1108
BstMAI GTCTC 2 cut(s) 784, 858
BstMBI GATC 3 cut(s) 529, 832, 1105
BstMCI CGRYCG 2 cut(s) 547, 559
BstMWI GCNNNNNNNGC 7 cut(s) 142, 356, 365, 374, 493, 722, 731
BstNI CCWGG 1 cut(s) 1068
BstNSI RCATGY 2 cut(s) 491, 684
BstSCI CCNGG 1 cut(s) 1066
BstV1I GCAGC 3 cut(s) 262, 352, 721
BstXI CCANNNNNNTGG 1 cut(s) 844
BsuRI GGCC 5 cut(s) 263, 408, 666, 929, 1083
BtgI CCRYGG 2 cut(s) 264, 924
BtsCI GGATG 2 cut(s) 202, 316
BtsI GCAGTG 1 cut(s) 159
BtsIMutI CAGTG 2 cut(s) 60, 159
BveI ACCTGC 1 cut(s) 423
Cac8I GCNNGC 1 cut(s) 714
CaiI CAGNNNCTG 1 cut(s) 734
Cfr13I GGNCC 3 cut(s) 344, 407, 770
Csp6I GTAC 2 cut(s) 87, 317
CviQI GTAC 2 cut(s) 87, 317
DdeI CTNAG 2 cut(s) 378, 626
DpnI GATC 3 cut(s) 531, 834, 1107
DpnII GATC 3 cut(s) 529, 832, 1105
EaeI YGGCCR 3 cut(s) 664, 927, 1081
Eam1104I CTCTTC 2 cut(s) 64, 1056
EarI CTCTTC 2 cut(s) 64, 1056
EciI GGCGGA 1 cut(s) 288
Ecl136II GAGCTC 1 cut(s) 23
Eco130I CCWWGG 3 cut(s) 264, 463, 924
Eco24I GRGCYC 2 cut(s) 25, 402
Eco31I GGTCTC 1 cut(s) 784
Eco47I GGWCC 2 cut(s) 344, 770
Eco53kI GAGCTC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 723
EcoICRI GAGCTC 1 cut(s) 23
EcoRI GAATTC 2 cut(s) 452, 1038
EcoRII CCWGG 1 cut(s) 1066
EcoT14I CCWWGG 3 cut(s) 264, 463, 924
EcoT38I GRGCYC 2 cut(s) 25, 402
ErhI CCWWGG 3 cut(s) 264, 463, 924
FalI AAGNNNNNCTT 2 cut(s) 173, 205
FaqI GGGAC 2 cut(s) 686, 919
FauI CCCGC 1 cut(s) 343
FauNDI CATATG 1 cut(s) 829
Fnu4HI GCNGC 3 cut(s) 251, 366, 735
FokI GGATG 2 cut(s) 209, 323
FriOI GRGCYC 2 cut(s) 25, 402
Fsp4HI GCNGC 3 cut(s) 251, 366, 735
FspBI CTAG 4 cut(s) 90, 470, 708, 1126
GluI GCNGC 3 cut(s) 251, 366, 735
HaeIII GGCC 5 cut(s) 263, 408, 666, 929, 1083
HapII CCGG 2 cut(s) 165, 347
HindIII AAGCTT 3 cut(s) 99, 293, 746
HinfI GANTC 3 cut(s) 15, 371, 608
HpaII CCGG 2 cut(s) 165, 347
HphI GGTGA 1 cut(s) 343
Hpy188I TCNGA 3 cut(s) 20, 27, 229
Hpy188III TCNNGA 2 cut(s) 515, 729
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 5 cut(s) 179, 208, 467, 591, 647
HpyCH4III ACNGT 5 cut(s) 46, 119, 276, 557, 721
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 3 cut(s) 250, 725, 754
HpyF10VI GCNNNNNNNGC 7 cut(s) 142, 356, 365, 374, 493, 722, 731
HpyF3I CTNAG 2 cut(s) 378, 626
HpySE526I ACGT 1 cut(s) 210
Kzo9I GATC 3 cut(s) 529, 832, 1105
LguI GCTCTTC 1 cut(s) 1056
LmnI GCTCC 6 cut(s) 20, 150, 229, 582, 586, 731
Lsp1109I GCAGC 3 cut(s) 262, 352, 721
LweI GCATC 2 cut(s) 734, 970
MaeI CTAG 4 cut(s) 90, 470, 708, 1126
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 128
MalI GATC 3 cut(s) 531, 834, 1107
MboI GATC 3 cut(s) 529, 832, 1105
MboII GAAGA 6 cut(s) 81, 215, 458, 1015, 1073, 1104
MhlI GDGCHC 2 cut(s) 25, 402
MlsI TGGCCA 3 cut(s) 666, 929, 1083
MluCI AATT 3 cut(s) 452, 571, 1038
MluNI TGGCCA 3 cut(s) 666, 929, 1083
MlyI GAGTC 2 cut(s) 380, 617
MmeI TCCRAC 1 cut(s) 207
MnlI CCTC 9 cut(s) 65, 193, 577, 715, 754, 799, 805, 949, 1087
Mox20I TGGCCA 3 cut(s) 666, 929, 1083
MscI TGGCCA 3 cut(s) 666, 929, 1083
MseI TTAA 1 cut(s) 1025
MslI CAYNNNNRTG 2 cut(s) 506, 929
Msp20I TGGCCA 3 cut(s) 666, 929, 1083
MspA1I CMGCKG 1 cut(s) 253
MspI CCGG 2 cut(s) 165, 347
MspR9I CCNGG 1 cut(s) 1068
Mva1269I GAATGC 1 cut(s) 136
MvaI CCWGG 1 cut(s) 1068
MwoI GCNNNNNNNGC 7 cut(s) 142, 356, 365, 374, 493, 722, 731
NcoI CCATGG 2 cut(s) 264, 924
NdeI CATATG 1 cut(s) 829
NdeII GATC 3 cut(s) 529, 832, 1105
NmeAIII GCCGAG 1 cut(s) 394
NspI RCATGY 2 cut(s) 491, 684
NspV TTCGAA 1 cut(s) 974
OliI CACNNNNGTG 1 cut(s) 506
PciSI GCTCTTC 1 cut(s) 1056
PcsI WCGNNNNNNNCGW 1 cut(s) 565
PctI GAATGC 1 cut(s) 136
PfeI GAWTC 1 cut(s) 15
PflFI GACNNNGTC 1 cut(s) 46
PkrI GCNGC 3 cut(s) 252, 367, 736
PleI GAGTC 2 cut(s) 379, 616
PpsI GAGTC 2 cut(s) 379, 616
Ppu21I YACGTR 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 1066
PspGI CCWGG 1 cut(s) 1066
PspPI GGNCC 3 cut(s) 344, 407, 770
PstNI CAGNNNCTG 1 cut(s) 734
PsyI GACNNNGTC 1 cut(s) 46
RsaI GTAC 2 cut(s) 88, 318
RsaNI GTAC 2 cut(s) 87, 317
RseI CAYNNNNRTG 2 cut(s) 506, 929
SacI GAGCTC 1 cut(s) 25
SapI GCTCTTC 1 cut(s) 1056
SaqAI TTAA 1 cut(s) 1025
SatI GCNGC 3 cut(s) 251, 366, 735
Sau3AI GATC 3 cut(s) 529, 832, 1105
Sau96I GGNCC 3 cut(s) 344, 407, 770
ScaI AGTACT 1 cut(s) 88
SchI GAGTC 2 cut(s) 380, 617
ScrFI CCNGG 1 cut(s) 1068
SduI GDGCHC 2 cut(s) 25, 402
SfaNI GCATC 2 cut(s) 734, 970
SfuI TTCGAA 1 cut(s) 974
SinI GGWCC 2 cut(s) 344, 770
SmiMI CAYNNNNRTG 2 cut(s) 506, 929
Sse9I AATT 3 cut(s) 452, 571, 1038
SsiI CCGC 5 cut(s) 194, 219, 253, 299, 350
SspI AATATT 1 cut(s) 948
SspMI CTAG 4 cut(s) 90, 470, 708, 1126
SstI GAGCTC 1 cut(s) 25
StyD4I CCNGG 1 cut(s) 1066
StyI CCWWGG 3 cut(s) 264, 463, 924
TaaI ACNGT 5 cut(s) 46, 119, 276, 557, 721
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 288, 974
TaqII GACCGA 1 cut(s) 533
TasI AATT 3 cut(s) 452, 571, 1038
TatI WGTACW 2 cut(s) 86, 316
TfiI GAWTC 1 cut(s) 15
Tru1I TTAA 1 cut(s) 1025
Tru9I TTAA 1 cut(s) 1025
TscAI CASTG 2 cut(s) 67, 166
TseI GCWGC 3 cut(s) 250, 365, 734
TspDTI ATGAA 5 cut(s) 465, 609, 841, 876, 1074
TspRI CASTG 2 cut(s) 67, 166
Tth111I GACNNNGTC 1 cut(s) 46
VpaK11BI GGWCC 2 cut(s) 344, 770
XapI RAATTY 3 cut(s) 452, 571, 1038
XceI RCATGY 2 cut(s) 491, 684
XcmI CCANNNNNNNNNTGG 1 cut(s) 794
XspI CTAG 4 cut(s) 90, 470, 708, 1126
ZrmI AGTACT 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.