MD02G1083500.v1.1

Lycopene epsilon cyclase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012912
Physical Location & Seq
Forward (+)
6504219 .. 6508025
3807 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1083500.v1.1.491

Sequence Viewer

Length: 1599 bp
ATGGACTGCGTTGCAGCAATGACGGCGTCGTTTTGCCCGACCTGGAGGGCGAGGAGAAGAGGAGGACTGGCGGGTGGAAAGGAAGGTCCTTTTTTCCGGTATGGTTTGTGCAACAAGGTTTCTCTGCTGCGAGTGAGAGCGAGTGCAGGAGGCGAAGGGGGCGGTGTTGGGGTGGCGGTGAAGGAAGGGTTTGCGGACGAGGAGGATTTCATGAAGGGCGGCGGGCCGGAACTGCTCTTAGTTCAAATGCAGCAGAAAAAGGTCATGGAGAAGCAGTCCAAGCTCGCTGAAAAGCTACTGCCAATACCAGTAGGAGATAGTGTTCTGGATTTGGTGGTGATTGGTTGTGGTCCGGCAGGTCTTGCTCTGGCTGCAGAATCAGCCAAGTTGGGATTAAAAGTTGGGCTTATTGGCCCTGATCTCCCTTTCACAAACAACTATGGTGTTTGGGAGGATGAATTCAAAGATCTTGGACTAGAAGGGTGTATTGAGCATGTTTGGCAGAACACCATAGTGTATCTTGACGGTGATTCGGACCCCATTATGATCGATCGTGCTTATGGTCGTGTTTGTCGAGACATGCTACACGAGGAGCTCTTGAGAAAGTGTGTGGAGTCAGGTGTTTCGTACCTCGATTCAAGGGTGGAAAGTATTGTTGAAGCTAGCAATGGGATTAGCCTTGTATCCTGTGGCCACAATATTGTTGTTTCGTGCAGGCTTGCTACTGTTGCATCAGGAGCTGCTTCGGGGAAGCTTTTGCAGTACGAGGTGGGTGGTCCAAAGGTGTCTGTCCAAACAGCATATGGTATCGAGGTTGAGGTGGAAAGTAACCCATATGATCCCGAAGTGATGGTTTTCATGGATTATAGAGACTACATGAAACAAAAAGTTCGGTCTTCAGAAGCAGAATATCCAACGTTTCTTTATGTCATGCCCATGTCTCCGACTAGATTGTTCTTTGAGGAAACTTGCTTGGCTTCTAAAGAAGCCATGCCTTTTGATTTATTGAAGAAAAAGCTCTTGTCACGGTTACAGACAATGGGAATCCGTATTACAAAAACTTATGAAGAGGAATGGTCTTGGATTCCGGTTGGTGGTTCCTTACCAAATACAGAGCAAAAGAACCTAGCATTTGGTGCTGCTGCTTGCATGGTACATCCTGCCACAGGATATTCAGTTGCAAGGTCTTTGTCGGAGGCTCCAAAATACGCTTCTGTGATTGCAACTATTTTGAAGCCAGATCATAATAAGGCCATTCGCAGCCGCCAAATAAGTAACGAGAACATCTCGATGCTAGCATGGAATACACTCTGGCCGCAAGAAAGGAAACGCCAAAGATCATTCTTTCTTTTCGGACTAGCGCTTATTCTGCAGCAGGACATCGAGGGCATCAGGATATTCTTTCACACTTTCTTCCGCTTACCCACCTGGATGTGGCAGGGTTTCCTCGGCTCTACGTTGTCCTCGGCTGATCTCATCCTTTTCGCGTTGTATATGTTTGTTTTAGCACCAAATAACTTGAGAAAGAACCTCGTCAGACACCTACTTTCTGATCCTTCCGGAGCCACTATGATTAGAACCTATTTCACATTCCATTAG

Protein Analysis

533

Amino Acids

58.99

Weight (kDa)

6.73

Isoelectric Point (pI)

47.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lycopene_cycl PF05834 110 - 508 8.4e-150 Lycopene cyclase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 347
AccII CGCG 1 cut(s) 1487
AccIII TCCGGA 1 cut(s) 1559
AciI CCGC 9 cut(s) 71, 162, 176, 194, 219, 222, 1264, 1316, 1417
AclI AACGTT 1 cut(s) 917
AclWI GGATC 2 cut(s) 833, 1547
AcoI YGGCCR 2 cut(s) 691, 1313
AcsI RAATTY 1 cut(s) 458
AcuI CTGAAG 1 cut(s) 882
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 3 cut(s) 629, 764, 1155
AfeI AGCGCT 1 cut(s) 1362
AfiI CCNNNNNNNGG 3 cut(s) 1094, 1166, 1434
AgsI TTSAA 6 cut(s) 245, 463, 639, 659, 1009, 1234
AjnI CCWGG 2 cut(s) 41, 1427
AjuI GAANNNNNNNTTGG 2 cut(s) 1195, 1227
AleI CACNNNNGTG 1 cut(s) 512
AloI GAACNNNNNNTCC 2 cut(s) 306, 338
AluBI AGCT 7 cut(s) 283, 295, 595, 662, 740, 754, 1018
AluI AGCT 7 cut(s) 283, 295, 595, 662, 740, 754, 1018
Alw21I GWGCWC 1 cut(s) 597
Alw26I GTCTC 3 cut(s) 570, 864, 945
AlwI GGATC 2 cut(s) 833, 1547
AlwNI CAGNNNCTG 1 cut(s) 740
Aor13HI TCCGGA 1 cut(s) 1559
Aor51HI AGCGCT 1 cut(s) 1362
AoxI GGCC 5 cut(s) 224, 412, 691, 1251, 1313
ApeKI GCWGC 9 cut(s) 14, 127, 250, 371, 740, 1139, 1142, 1260, 1372
ApoI RAATTY 1 cut(s) 458
AspLEI GCGC 1 cut(s) 1363
AspS9I GGNCC 6 cut(s) 86, 224, 350, 413, 535, 776
AsuHPI GGTGA 3 cut(s) 190, 349, 539
AsuNHI GCTAGC 2 cut(s) 662, 1294
AvaII GGWCC 4 cut(s) 86, 350, 535, 776
BalI TGGCCA 1 cut(s) 693
BanII GRGCYC 1 cut(s) 597
BauI CACGAG 1 cut(s) 587
BbsI GAAGAC 1 cut(s) 888
Bbv12I GWGCWC 1 cut(s) 597
BbvI GCAGC 9 cut(s) 26, 114, 262, 358, 727, 1126, 1129, 1272, 1384
BccI CCATC 1 cut(s) 844
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 2 cut(s) 43, 1429
BciVI GTATCC 1 cut(s) 694
BcoDI GTCTC 3 cut(s) 570, 864, 945
BfaI CTAG 6 cut(s) 476, 663, 948, 1127, 1295, 1358
BfmI CTRYAG 2 cut(s) 372, 1370
BfoI RGCGCY 1 cut(s) 1364
BfuAI ACCTGC 1 cut(s) 347
BfuI GTATCC 1 cut(s) 694
BglII AGATCT 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 43, 1429
Bme18I GGWCC 4 cut(s) 86, 350, 535, 776
BmgT120I GGNCC 6 cut(s) 86, 224, 350, 413, 535, 776
BmiI GGNNCC 4 cut(s) 537, 1099, 1200, 1564
BmrFI CCNGG 2 cut(s) 43, 1429
BmsI GCATC 3 cut(s) 740, 1281, 1398
BmtI GCTAGC 2 cut(s) 666, 1298
BpiI GAAGAC 1 cut(s) 888
BplI GAGNNNNNCTC 2 cut(s) 1271, 1303
BpmI CTGGAG 1 cut(s) 64
BpuEI CTTGAG 2 cut(s) 619, 1540
Bsa29I ATCGAT 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 1447, 1464
BsaWI WCCGGW 3 cut(s) 96, 1087, 1559
BsaXI ACNNNNNCTCC 4 cut(s) 260, 290, 306, 336
Bsc4I CCNNNNNNNGG 3 cut(s) 1094, 1166, 1434
Bse1I ACTGG 2 cut(s) 72, 308
Bse3DI GCAATG 2 cut(s) 24, 673
BseAI TCCGGA 1 cut(s) 1559
BseBI CCWGG 2 cut(s) 43, 1429
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 1447, 1464
BseGI GGATG 4 cut(s) 460, 1156, 1437, 1476
BseLI CCNNNNNNNGG 3 cut(s) 1094, 1166, 1434
BseMI GCAATG 2 cut(s) 24, 673
BseNI ACTGG 2 cut(s) 72, 308
BseRI GAGGAG 4 cut(s) 67, 75, 215, 605
BseXI GCAGC 9 cut(s) 26, 114, 262, 358, 727, 1126, 1129, 1272, 1384
BsgI GTGCAG 2 cut(s) 165, 733
Bsh1236I CGCG 1 cut(s) 1487
Bsh1285I CGRYCG 1 cut(s) 553
BshFI GGCC 5 cut(s) 226, 414, 693, 1253, 1315
BshVI ATCGAT 1 cut(s) 549
BsiEI CGRYCG 1 cut(s) 553
BsiHKAI GWGCWC 1 cut(s) 597
BsiSI CCGG 5 cut(s) 97, 227, 353, 1088, 1560
BslI CCNNNNNNNGG 3 cut(s) 1094, 1166, 1434
BsmAI GTCTC 3 cut(s) 570, 864, 945
BsnI GGCC 5 cut(s) 226, 414, 693, 1253, 1315
Bsp1286I GDGCHC 1 cut(s) 597
Bsp13I TCCGGA 1 cut(s) 1559
Bsp143I GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
BspACI CCGC 9 cut(s) 71, 162, 176, 194, 219, 222, 1264, 1316, 1417
BspANI GGCC 5 cut(s) 226, 414, 693, 1253, 1315
BspDI ATCGAT 1 cut(s) 549
BspEI TCCGGA 1 cut(s) 1559
BspFNI CGCG 1 cut(s) 1487
BspHI TCATGA 1 cut(s) 210
BspLI GGNNCC 4 cut(s) 537, 1099, 1200, 1564
BspMAI CTGCAG 2 cut(s) 376, 1374
BspMI ACCTGC 1 cut(s) 347
BspOI GCTAGC 2 cut(s) 666, 1298
BspPI GGATC 2 cut(s) 833, 1547
BsrDI GCAATG 2 cut(s) 24, 673
BsrI ACTGG 2 cut(s) 72, 308
BssECI CCNNGG 2 cut(s) 1447, 1464
BssMI GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
BssNI GRCGYC 1 cut(s) 26
BssSI CACGAG 1 cut(s) 587
Bst2BI CACGAG 1 cut(s) 587
Bst2UI CCWGG 2 cut(s) 43, 1429
Bst4CI ACNGT 3 cut(s) 527, 727, 1029
Bst6I CTCTTC 2 cut(s) 52, 1062
BstACI GRCGYC 1 cut(s) 26
BstAPI GCANNNNNTGC 2 cut(s) 362, 1136
BstC8I GCNNGC 7 cut(s) 224, 285, 664, 716, 720, 1147, 1296
BstDEI CTNAG 1 cut(s) 238
BstENI CCTNNNNNAGG 1 cut(s) 1164
BstF5I GGATG 4 cut(s) 460, 1156, 1437, 1476
BstFNI CGCG 1 cut(s) 1487
BstH2I RGCGCY 1 cut(s) 1364
BstHHI GCGC 1 cut(s) 1363
BstKTI GATC 9 cut(s) 421, 469, 549, 553, 841, 1243, 1340, 1474, 1555
BstMAI GTCTC 3 cut(s) 570, 864, 945
BstMBI GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
BstMCI CGRYCG 1 cut(s) 553
BstNI CCWGG 2 cut(s) 43, 1429
BstNSI RCATGY 2 cut(s) 497, 583
BstSCI CCNGG 2 cut(s) 41, 1427
BstSFI CTRYAG 2 cut(s) 372, 1370
BstUI CGCG 1 cut(s) 1487
BstV1I GCAGC 9 cut(s) 26, 114, 262, 358, 727, 1126, 1129, 1272, 1384
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
Bsu15I ATCGAT 1 cut(s) 549
BsuI GTATCC 1 cut(s) 694
BsuRI GGCC 5 cut(s) 226, 414, 693, 1253, 1315
BsuTUI ATCGAT 1 cut(s) 549
BtsCI GGATG 4 cut(s) 460, 1156, 1437, 1476
BveI ACCTGC 1 cut(s) 347
Cac8I GCNNGC 7 cut(s) 224, 285, 664, 716, 720, 1147, 1296
CaiI CAGNNNCTG 1 cut(s) 740
CciI TCATGA 1 cut(s) 210
CfoI GCGC 1 cut(s) 1363
Cfr13I GGNCC 6 cut(s) 86, 224, 350, 413, 535, 776
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 1 cut(s) 15
Csp6I GTAC 3 cut(s) 628, 763, 1154
CviQI GTAC 3 cut(s) 628, 763, 1154
DdeI CTNAG 1 cut(s) 238
DpnI GATC 9 cut(s) 420, 468, 548, 552, 840, 1242, 1339, 1473, 1554
DpnII GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
EaeI YGGCCR 2 cut(s) 691, 1313
Eam1104I CTCTTC 2 cut(s) 52, 1062
EarI CTCTTC 2 cut(s) 52, 1062
Ecl136II GAGCTC 1 cut(s) 595
Eco24I GRGCYC 1 cut(s) 597
Eco47I GGWCC 4 cut(s) 86, 350, 535, 776
Eco47III AGCGCT 1 cut(s) 1362
Eco53kI GAGCTC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 882
EcoICRI GAGCTC 1 cut(s) 595
EcoNI CCTNNNNNAGG 1 cut(s) 1164
EcoO109I RGGNCCY 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 2 cut(s) 41, 1427
EcoT38I GRGCYC 1 cut(s) 597
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FauI CCCGC 2 cut(s) 64, 215
FauNDI CATATG 2 cut(s) 802, 835
FokI GGATG 4 cut(s) 467, 1143, 1444, 1463
FriOI GRGCYC 1 cut(s) 597
FspBI CTAG 6 cut(s) 476, 663, 948, 1127, 1295, 1358
GlaI GCGC 1 cut(s) 1362
GsuI CTGGAG 1 cut(s) 64
HaeII RGCGCY 1 cut(s) 1364
HaeIII GGCC 5 cut(s) 226, 414, 693, 1253, 1315
HapII CCGG 5 cut(s) 97, 227, 353, 1088, 1560
HgaI GACGC 1 cut(s) 15
HhaI GCGC 1 cut(s) 1363
Hin1I GRCGYC 1 cut(s) 26
Hin6I GCGC 1 cut(s) 1361
HinP1I GCGC 1 cut(s) 1361
HindIII AAGCTT 1 cut(s) 752
HinfI GANTC 6 cut(s) 377, 530, 614, 635, 1044, 1084
HpaII CCGG 5 cut(s) 97, 227, 353, 1088, 1560
HphI GGTGA 3 cut(s) 190, 349, 539
Hpy188I TCNGA 7 cut(s) 535, 901, 945, 1195, 1355, 1538, 1552
Hpy99I CGWCG 1 cut(s) 31
HpyAV CCTTC 7 cut(s) 77, 149, 175, 179, 208, 473, 1566
HpyCH4III ACNGT 3 cut(s) 527, 727, 1029
HpyCH4IV ACGT 2 cut(s) 917, 1457
HpyF3I CTNAG 1 cut(s) 238
HpySE526I ACGT 2 cut(s) 917, 1457
Hsp92I GRCGYC 1 cut(s) 26
HspAI GCGC 1 cut(s) 1361
Kpn2I TCCGGA 1 cut(s) 1559
Kzo9I GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
LmnI GCTCC 4 cut(s) 592, 737, 1204, 1562
Lsp1109I GCAGC 9 cut(s) 26, 114, 262, 358, 727, 1126, 1129, 1272, 1384
LweI GCATC 3 cut(s) 740, 1281, 1398
MaeI CTAG 6 cut(s) 476, 663, 948, 1127, 1295, 1358
MaeII ACGT 2 cut(s) 917, 1457
MaeIII GTNAC 4 cut(s) 827, 1023, 1029, 1274
MalI GATC 9 cut(s) 420, 468, 548, 552, 840, 1242, 1339, 1473, 1554
MboI GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
MboII GAAGA 5 cut(s) 69, 888, 1021, 1079, 1405
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 693
MluCI AATT 1 cut(s) 458
MluNI TGGCCA 1 cut(s) 693
MlyI GAGTC 1 cut(s) 623
MmeI TCCRAC 3 cut(s) 938, 968, 1173
Mox20I TGGCCA 1 cut(s) 693
MroI TCCGGA 1 cut(s) 1559
MscI TGGCCA 1 cut(s) 693
MseI TTAA 1 cut(s) 395
MslI CAYNNNNRTG 4 cut(s) 512, 935, 1289, 1430
Msp20I TGGCCA 1 cut(s) 693
MspI CCGG 5 cut(s) 97, 227, 353, 1088, 1560
MspR9I CCNGG 2 cut(s) 43, 1429
MvaI CCWGG 2 cut(s) 43, 1429
MvnI CGCG 1 cut(s) 1487
NdeI CATATG 2 cut(s) 802, 835
NdeII GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
NheI GCTAGC 2 cut(s) 662, 1294
NlaIV GGNNCC 4 cut(s) 537, 1099, 1200, 1564
NmeAIII GCCGAG 2 cut(s) 1428, 1445
NmuCI GTSAC 1 cut(s) 1023
NspI RCATGY 2 cut(s) 497, 583
OliI CACNNNNGTG 1 cut(s) 512
PagI TCATGA 1 cut(s) 210
PcsI WCGNNNNNNNCGW 2 cut(s) 35, 571
PfeI GAWTC 5 cut(s) 377, 530, 635, 1044, 1084
PflFI GACNNNGTC 1 cut(s) 25
Ple19I CGATCG 1 cut(s) 553
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
PpuMI RGGWCCY 1 cut(s) 86
Psp124BI GAGCTC 1 cut(s) 597
Psp1406I AACGTT 1 cut(s) 917
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 2 cut(s) 41, 1427
PspGI CCWGG 2 cut(s) 41, 1427
PspN4I GGNNCC 4 cut(s) 537, 1099, 1200, 1564
PspPI GGNCC 6 cut(s) 86, 224, 350, 413, 535, 776
PspPPI RGGWCCY 1 cut(s) 86
PstI CTGCAG 2 cut(s) 376, 1374
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 1 cut(s) 466
PsyI GACNNNGTC 1 cut(s) 25
PvuI CGATCG 1 cut(s) 553
RsaI GTAC 3 cut(s) 629, 764, 1155
RsaNI GTAC 3 cut(s) 628, 763, 1154
RseI CAYNNNNRTG 4 cut(s) 512, 935, 1289, 1430
SacI GAGCTC 1 cut(s) 597
SaqAI TTAA 1 cut(s) 395
Sau3AI GATC 9 cut(s) 418, 466, 546, 550, 838, 1240, 1337, 1471, 1552
Sau96I GGNCC 6 cut(s) 86, 224, 350, 413, 535, 776
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 43, 1429
SduI GDGCHC 1 cut(s) 597
SfaNI GCATC 3 cut(s) 740, 1281, 1398
SfcI CTRYAG 2 cut(s) 372, 1370
SinI GGWCC 4 cut(s) 86, 350, 535, 776
SmiMI CAYNNNNRTG 4 cut(s) 512, 935, 1289, 1430
SmlI CTYRAG 2 cut(s) 598, 1519
SmoI CTYRAG 2 cut(s) 598, 1519
Sse9I AATT 1 cut(s) 458
SsiI CCGC 9 cut(s) 71, 162, 176, 194, 219, 222, 1264, 1316, 1417
SspI AATATT 1 cut(s) 700
SspMI CTAG 6 cut(s) 476, 663, 948, 1127, 1295, 1358
SstI GAGCTC 1 cut(s) 597
StyD4I CCNGG 2 cut(s) 41, 1427
TaaI ACNGT 3 cut(s) 527, 727, 1029
TaiI ACGT 2 cut(s) 920, 1460
TaqI TCGA 6 cut(s) 549, 574, 633, 810, 1289, 1383
TaqII GACCGA 1 cut(s) 882
TasI AATT 1 cut(s) 458
TauI GCSGC 3 cut(s) 222, 1266, 1318
TfiI GAWTC 5 cut(s) 377, 530, 635, 1044, 1084
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TseFI GTSAC 1 cut(s) 1023
TseI GCWGC 9 cut(s) 14, 127, 250, 371, 740, 1139, 1142, 1260, 1372
Tsp45I GTSAC 1 cut(s) 1023
TspDTI ATGAA 6 cut(s) 199, 227, 471, 847, 893, 1080
TspGWI ACGGA 1 cut(s) 1037
Tth111I GACNNNGTC 1 cut(s) 25
VpaK11BI GGWCC 4 cut(s) 86, 350, 535, 776
XagI CCTNNNNNAGG 1 cut(s) 1164
XapI RAATTY 1 cut(s) 458
XceI RCATGY 2 cut(s) 497, 583
XcmI CCANNNNNNNNNTGG 1 cut(s) 800
XspI CTAG 6 cut(s) 476, 663, 948, 1127, 1295, 1358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.