FvH4_1g07950

Lycopene epsilon cyclase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012912
Physical Location & Seq
Forward (+)
4197480 .. 4201172
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g07950.t1

Sequence Viewer

Length: 1632 bp
ATGGACTGCGTTGGAATCGGAGCTCGGAACTTTACGGCAATGACGGTGTCGTTTTGCCCGGCTGGGACACGAGGGTTTGTCCCCAACTTTAAAAAGGAGCCTTTCTTCTTTCGTTACGGCGCTTTGTGTAGTAACAAGCATTCTCTGCTTCAAGTGAGAGCCACTGCCGGAAGCGAGAGCTATGTGGGTGTGGCTGTGAAAGAAGATTTTGCTGACGAGGAGGATTACGTCAAGGGCGGTGGATCGGAGCTGCTTTTCGTTCAAATGCAGCGGAACAAGATCATGGAGAAGCAGTCCAAACTCGCTGATAAGCTAAGGCCAATAGGAGATGATATATTGGATTTGGTGGTGATTGGTTGCGGTCCGGCTGGTCTTGCTCTGGCTGCAGAGTCGGCTAAGTTGGGGCTGAAAGTTGGGCTGGTTGGCCCTGACTTGCCTTTCACTAACAACTATGGTGTTTGGGAAGATGAATTCAAAGACCTTGGACTAGAAGGGTGCATTGAGCATGTTTGGCAGGACACGATTGTGTATCTTGATGATGAGAGTGAACCGATTATGTTTGGTCGTGCTTACGGCCGTGTGTGTCGAGATTTGCTCCATGAGGAGCTTTTAAGAAGGTGTGTAGAGTCAGGAGTTTCATACCTTTGTTCAAGGGTGGAGAGTATTGTTGAAGGTAGCAATGGCCATAGCCTTATAGCATGTGAGCATGGTATCAACATCTCCTCTAGGCTGGCTACTGTTGCATCAGGAGCTGCTTCAGGGAAGCTTTTGCAATATGAGGTGGGTGGTCCAAAGGTCTGTGTCCAGACAGCTTATGGAGTGGAAGTTGAGGTGGAAAATTATCCATATGATCCCAACCTGATGGTTTTCATGGATTATAGAGACTACACAAAACAAAAAGTTCCATGTTTGGAAGCTGAATATCCAACATTTCTTTATGCCATGGCCATGTCCCCAACAAAAATATTCTTTGAGGAAACTTGCTTGGCTTCGAAAGATGCCATGCCTTTTGATTTACTGAAGAAAAAGCTTTTCTCAAGGTTGAATACACTGGGAATCCGAATTGTAAAAACTTATGAAGAGGAATGGTCTTGGATTCCAGTTGGGGGGTCATTACCGAATACAGAGCAAAAGAACTTGGCATTTGGTGCTGCTGCTAGCATGGTGCATCCAGCCACAGGGTATTCAGTTGTGAGATCTTTATCAGAGGCTCCAAAATATGCTACTGTGATTGCAAAGATTTTGAAGCAGGGTCACTCAAACGCCAGTCTTAGCCATCAAATAAGTAATCAGAATATCTCAATGCAAGCTTGGAATACCCTCTGGCCACAAGAAAGGAAACGCCAAAGAGCATTCTTTTTGTTTGGACTAGCGCTCCTTGTGCAAATGGACATTGAGGGCATGCGGACATTCTTCGCTACCTTCTTCCGCTTACCTACCTGGATGTGGCAGGGTTTTCTGGGTTCATCTTTGTCCTCGGTCGATCTCATGCTCTTTGCCTTGTATATGTTTGTTATAGCACCAAATAGTTTGAGAAGTCGCCTTGTCAAACATCTATTGTCAGATCCTACTGGAGCCACCATGTTAAAAACCTATCTCACAGTAAACTTGAATTCCTTCCCTAGAACTTAG

Protein Analysis

544

Amino Acids

60.41

Weight (kDa)

6.2

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lycopene_cycl PF05834 114 - 512 1.5e-149 Lycopene cyclase protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 237, 271, 360, 1405, 1429
AclWI GGATC 3 cut(s) 250, 845, 1559
AcoI YGGCCR 4 cut(s) 574, 682, 945, 1325
AcsI RAATTY 2 cut(s) 470, 1612
AcuI CTGAAG 2 cut(s) 741, 1040
AfeI AGCGCT 1 cut(s) 1374
AfiI CCNNNNNNNGG 3 cut(s) 1106, 1178, 1446
AgsI TTSAA 8 cut(s) 152, 263, 475, 651, 671, 1045, 1246, 1612
AjnI CCWGG 1 cut(s) 1439
AleI CACNNNNGTG 1 cut(s) 524
Alw21I GWGCWC 1 cut(s) 25
Alw26I GTCTC 1 cut(s) 876
AlwI GGATC 3 cut(s) 250, 845, 1559
AlwNI CAGNNNCTG 1 cut(s) 752
Aor51HI AGCGCT 1 cut(s) 1374
AoxI GGCC 6 cut(s) 317, 424, 574, 682, 945, 1325
ApeKI GCWGC 6 cut(s) 250, 268, 383, 752, 1151, 1154
ApoI RAATTY 2 cut(s) 470, 1612
Asp700I GAANNNNTTC 1 cut(s) 1616
AspLEI GCGC 2 cut(s) 122, 1375
AspS9I GGNCC 3 cut(s) 362, 425, 788
AsuC2I CCSGG 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 361
AsuII TTCGAA 1 cut(s) 992
AsuNHI GCTAGC 1 cut(s) 1157
AvaII GGWCC 2 cut(s) 362, 788
BalI TGGCCA 3 cut(s) 684, 947, 1327
BanII GRGCYC 1 cut(s) 25
BauI CACGAG 1 cut(s) 69
Bbv12I GWGCWC 1 cut(s) 25
BbvI GCAGC 6 cut(s) 237, 280, 370, 739, 1138, 1141
BccI CCATC 2 cut(s) 856, 1284
BceAI ACGGC 4 cut(s) 51, 133, 561, 589
BciT130I CCWGG 1 cut(s) 1441
BcnI CCSGG 1 cut(s) 59
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 5 cut(s) 488, 726, 1158, 1370, 1623
BfmI CTRYAG 1 cut(s) 384
BfoI RGCGCY 2 cut(s) 123, 1376
BglII AGATCT 1 cut(s) 1196
BisI GCNGC 6 cut(s) 251, 269, 384, 753, 1152, 1155
BlsI GCNGC 6 cut(s) 252, 270, 385, 754, 1153, 1156
Bme1390I CCNGG 2 cut(s) 59, 1441
Bme18I GGWCC 2 cut(s) 362, 788
BmgT120I GGNCC 3 cut(s) 362, 425, 788
BmiI GGNNCC 3 cut(s) 99, 1212, 1576
BmrFI CCNGG 2 cut(s) 59, 1441
BmrI ACTGGG 1 cut(s) 1061
BmsI GCATC 3 cut(s) 752, 988, 1177
BmtI GCTAGC 1 cut(s) 1161
BmuI ACTGGG 1 cut(s) 1061
BplI GAGNNNNNCTC 2 cut(s) 579, 611
BpmI CTGGAG 1 cut(s) 1593
Bpu10I CCTNAGC 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 992
BpuEI CTTGAG 1 cut(s) 1021
BpuMI CCSGG 1 cut(s) 59
BsaBI GATNNNNATC 1 cut(s) 1201
BsaJI CCNNGG 3 cut(s) 481, 942, 1476
BsaXI ACNNNNNCTCC 6 cut(s) 278, 308, 810, 840, 1359, 1389
Bsc4I CCNNNNNNNGG 3 cut(s) 1106, 1178, 1446
Bse1I ACTGG 4 cut(s) 1056, 1100, 1266, 1576
Bse3DI GCAATG 2 cut(s) 45, 685
Bse8I GATNNNNATC 1 cut(s) 1201
BseBI CCWGG 1 cut(s) 1441
BseDI CCNNGG 3 cut(s) 481, 942, 1476
BseGI GGATG 2 cut(s) 1168, 1449
BseJI GATNNNNATC 1 cut(s) 1201
BseLI CCNNNNNNNGG 3 cut(s) 1106, 1178, 1446
BseMI GCAATG 2 cut(s) 45, 685
BseNI ACTGG 4 cut(s) 1056, 1100, 1266, 1576
BseRI GAGGAG 3 cut(s) 233, 617, 712
BseX3I CGGCCG 1 cut(s) 574
BseXI GCAGC 6 cut(s) 237, 280, 370, 739, 1138, 1141
BseYI CCCAGC 1 cut(s) 62
Bsh1285I CGRYCG 2 cut(s) 577, 1482
BshFI GGCC 6 cut(s) 319, 426, 576, 684, 947, 1327
BsiEI CGRYCG 2 cut(s) 577, 1482
BsiHKAI GWGCWC 1 cut(s) 25
BsiSI CCGG 3 cut(s) 59, 168, 365
BslFI GGGAC 3 cut(s) 65, 79, 937
BslI CCNNNNNNNGG 3 cut(s) 1106, 1178, 1446
BsmAI GTCTC 1 cut(s) 876
BsmFI GGGAC 3 cut(s) 65, 79, 937
BsmI GAATGC 2 cut(s) 139, 1352
BsnI GGCC 6 cut(s) 319, 426, 576, 684, 947, 1327
Bsp119I TTCGAA 1 cut(s) 992
Bsp1286I GDGCHC 1 cut(s) 25
Bsp143I GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
Bsp19I CCATGG 1 cut(s) 942
BspACI CCGC 5 cut(s) 237, 271, 360, 1405, 1429
BspANI GGCC 6 cut(s) 319, 426, 576, 684, 947, 1327
BspLI GGNNCC 3 cut(s) 99, 1212, 1576
BspMAI CTGCAG 1 cut(s) 388
BspOI GCTAGC 1 cut(s) 1161
BspPI GGATC 3 cut(s) 250, 845, 1559
BspT104I TTCGAA 1 cut(s) 992
BsrDI GCAATG 2 cut(s) 45, 685
BsrI ACTGG 4 cut(s) 1056, 1100, 1266, 1576
BssECI CCNNGG 3 cut(s) 481, 942, 1476
BssMI GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
BssSI CACGAG 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 481, 942
Bst2BI CACGAG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 1441
Bst4CI ACNGT 4 cut(s) 46, 739, 1228, 1603
Bst6I CTCTTC 1 cut(s) 1074
BstAPI GCANNNNNTGC 2 cut(s) 145, 1148
BstBI TTCGAA 1 cut(s) 992
BstC8I GCNNGC 4 cut(s) 732, 1159, 1308, 1403
BstDEI CTNAG 4 cut(s) 314, 396, 1271, 1629
BstDSI CCRYGG 1 cut(s) 942
BstF5I GGATG 2 cut(s) 1168, 1449
BstH2I RGCGCY 2 cut(s) 123, 1376
BstHHI GCGC 2 cut(s) 122, 1375
BstKTI GATC 6 cut(s) 245, 282, 853, 1199, 1486, 1567
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
BstMCI CGRYCG 2 cut(s) 577, 1482
BstMWI GCNNNNNNNGC 9 cut(s) 145, 374, 383, 392, 511, 740, 749, 1148, 1381
BstNI CCWGG 1 cut(s) 1441
BstNSI RCATGY 3 cut(s) 509, 702, 1405
BstSCI CCNGG 2 cut(s) 57, 1439
BstSFI CTRYAG 1 cut(s) 384
BstV1I GCAGC 6 cut(s) 237, 280, 370, 739, 1138, 1141
BstX2I RGATCY 2 cut(s) 1196, 1564
BstXI CCANNNNNNTGG 1 cut(s) 862
BstYI RGATCY 2 cut(s) 1196, 1564
BstZI CGGCCG 1 cut(s) 574
BsuRI GGCC 6 cut(s) 319, 426, 576, 684, 947, 1327
BtgI CCRYGG 1 cut(s) 942
BtsCI GGATG 2 cut(s) 1168, 1449
BtsI GCAGTG 1 cut(s) 162
BtsIMutI CAGTG 2 cut(s) 162, 1049
Cac8I GCNNGC 4 cut(s) 732, 1159, 1308, 1403
CaiI CAGNNNCTG 1 cut(s) 752
CfoI GCGC 2 cut(s) 122, 1375
Cfr13I GGNCC 3 cut(s) 362, 425, 788
CpoI CGGWCCG 1 cut(s) 362
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CspI CGGWCCG 1 cut(s) 362
DdeI CTNAG 4 cut(s) 314, 396, 1271, 1629
DpnI GATC 6 cut(s) 244, 281, 852, 1198, 1485, 1566
DpnII GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
DraI TTTAAA 1 cut(s) 91
EaeI YGGCCR 4 cut(s) 574, 682, 945, 1325
EagI CGGCCG 1 cut(s) 574
Eam1104I CTCTTC 1 cut(s) 1074
EarI CTCTTC 1 cut(s) 1074
Ecl136II GAGCTC 1 cut(s) 23
EclXI CGGCCG 1 cut(s) 574
Eco130I CCWWGG 2 cut(s) 481, 942
Eco24I GRGCYC 1 cut(s) 25
Eco47I GGWCC 2 cut(s) 362, 788
Eco47III AGCGCT 1 cut(s) 1374
Eco52I CGGCCG 1 cut(s) 574
Eco53kI GAGCTC 1 cut(s) 23
Eco57I CTGAAG 2 cut(s) 741, 1040
EcoICRI GAGCTC 1 cut(s) 23
EcoRI GAATTC 2 cut(s) 470, 1612
EcoRII CCWGG 1 cut(s) 1439
EcoT14I CCWWGG 2 cut(s) 481, 942
EcoT38I GRGCYC 1 cut(s) 25
ErhI CCWWGG 2 cut(s) 481, 942
FaqI GGGAC 3 cut(s) 65, 79, 937
FauNDI CATATG 1 cut(s) 847
Fnu4HI GCNGC 6 cut(s) 251, 269, 384, 753, 1152, 1155
FokI GGATG 2 cut(s) 1155, 1456
FriOI GRGCYC 1 cut(s) 25
Fsp4HI GCNGC 6 cut(s) 251, 269, 384, 753, 1152, 1155
FspBI CTAG 5 cut(s) 488, 726, 1158, 1370, 1623
GlaI GCGC 2 cut(s) 121, 1374
GluI GCNGC 6 cut(s) 251, 269, 384, 753, 1152, 1155
GsaI CCCAGC 1 cut(s) 66
GsuI CTGGAG 1 cut(s) 1593
HaeII RGCGCY 2 cut(s) 123, 1376
HaeIII GGCC 6 cut(s) 319, 426, 576, 684, 947, 1327
HapII CCGG 3 cut(s) 59, 168, 365
HhaI GCGC 2 cut(s) 122, 1375
Hin6I GCGC 2 cut(s) 120, 1373
HinP1I GCGC 2 cut(s) 120, 1373
HindIII AAGCTT 3 cut(s) 764, 1028, 1308
HinfI GANTC 5 cut(s) 15, 389, 626, 1056, 1096
HpaII CCGG 3 cut(s) 59, 168, 365
HphI GGTGA 1 cut(s) 361
Hpy166II GTNNAC 2 cut(s) 548, 1606
Hpy188I TCNGA 7 cut(s) 20, 27, 247, 1061, 1207, 1293, 1564
Hpy188III TCNNGA 5 cut(s) 533, 587, 630, 747, 805
Hpy8I GTNNAC 2 cut(s) 548, 1606
HpyAV CCTTC 5 cut(s) 485, 609, 665, 1432, 1627
HpyCH4III ACNGT 4 cut(s) 46, 739, 1228, 1603
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 9 cut(s) 268, 386, 498, 743, 772, 1168, 1235, 1306, 1384
HpyF10VI GCNNNNNNNGC 9 cut(s) 145, 374, 383, 392, 511, 740, 749, 1148, 1381
HpyF3I CTNAG 4 cut(s) 314, 396, 1271, 1629
HpySE526I ACGT 1 cut(s) 228
HspAI GCGC 2 cut(s) 120, 1373
Kzo9I GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
LmnI GCTCC 9 cut(s) 20, 97, 247, 600, 604, 749, 1216, 1380, 1574
Lsp1109I GCAGC 6 cut(s) 237, 280, 370, 739, 1138, 1141
LweI GCATC 3 cut(s) 752, 988, 1177
MaeI CTAG 5 cut(s) 488, 726, 1158, 1370, 1623
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 3 cut(s) 113, 131, 1253
MalI GATC 6 cut(s) 244, 281, 852, 1198, 1485, 1566
MboI GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
MboII GAAGA 7 cut(s) 97, 215, 476, 1033, 1091, 1405, 1417
MflI RGATCY 2 cut(s) 1196, 1564
MhlI GDGCHC 1 cut(s) 25
MlsI TGGCCA 3 cut(s) 684, 947, 1327
MluCI AATT 4 cut(s) 470, 838, 1062, 1612
MluNI TGGCCA 3 cut(s) 684, 947, 1327
MlyI GAGTC 2 cut(s) 398, 635
MmeI TCCRAC 1 cut(s) 950
Mox20I TGGCCA 3 cut(s) 684, 947, 1327
MroXI GAANNNNTTC 1 cut(s) 1616
MscI TGGCCA 3 cut(s) 684, 947, 1327
MseI TTAA 3 cut(s) 90, 611, 1586
MslI CAYNNNNRTG 2 cut(s) 524, 947
Msp20I TGGCCA 3 cut(s) 684, 947, 1327
MspA1I CMGCKG 1 cut(s) 271
MspI CCGG 3 cut(s) 59, 168, 365
MspR9I CCNGG 2 cut(s) 59, 1441
Mva1269I GAATGC 2 cut(s) 139, 1352
MvaI CCWGG 1 cut(s) 1441
MwoI GCNNNNNNNGC 9 cut(s) 145, 374, 383, 392, 511, 740, 749, 1148, 1381
NciI CCSGG 1 cut(s) 59
NcoI CCATGG 1 cut(s) 942
NdeI CATATG 1 cut(s) 847
NdeII GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
NheI GCTAGC 1 cut(s) 1157
NlaIV GGNNCC 3 cut(s) 99, 1212, 1576
NmuCI GTSAC 1 cut(s) 1253
NspI RCATGY 3 cut(s) 509, 702, 1405
NspV TTCGAA 1 cut(s) 992
OliI CACNNNNGTG 1 cut(s) 524
PaeI GCATGC 1 cut(s) 1405
PctI GAATGC 2 cut(s) 139, 1352
PdmI GAANNNNTTC 1 cut(s) 1616
PfeI GAWTC 3 cut(s) 15, 1056, 1096
PflFI GACNNNGTC 1 cut(s) 46
PkrI GCNGC 6 cut(s) 252, 270, 385, 754, 1153, 1156
PleI GAGTC 2 cut(s) 397, 634
PpsI GAGTC 2 cut(s) 397, 634
Psp124BI GAGCTC 1 cut(s) 25
Psp6I CCWGG 1 cut(s) 1439
PspFI CCCAGC 1 cut(s) 62
PspGI CCWGG 1 cut(s) 1439
PspN4I GGNNCC 3 cut(s) 99, 1212, 1576
PspPI GGNCC 3 cut(s) 362, 425, 788
PstI CTGCAG 1 cut(s) 388
PstNI CAGNNNCTG 1 cut(s) 752
PsuI RGATCY 2 cut(s) 1196, 1564
PsyI GACNNNGTC 1 cut(s) 46
RseI CAYNNNNRTG 2 cut(s) 524, 947
Rsr2I CGGWCCG 1 cut(s) 362
RsrII CGGWCCG 1 cut(s) 362
SacI GAGCTC 1 cut(s) 25
SaqAI TTAA 3 cut(s) 90, 611, 1586
SatI GCNGC 6 cut(s) 251, 269, 384, 753, 1152, 1155
Sau3AI GATC 6 cut(s) 242, 279, 850, 1196, 1483, 1564
Sau96I GGNCC 3 cut(s) 362, 425, 788
SchI GAGTC 2 cut(s) 398, 635
ScrFI CCNGG 2 cut(s) 59, 1441
SduI GDGCHC 1 cut(s) 25
SfaNI GCATC 3 cut(s) 752, 988, 1177
SfcI CTRYAG 1 cut(s) 384
SfuI TTCGAA 1 cut(s) 992
SinI GGWCC 2 cut(s) 362, 788
SmiMI CAYNNNNRTG 2 cut(s) 524, 947
SmlI CTYRAG 1 cut(s) 1036
SmoI CTYRAG 1 cut(s) 1036
SphI GCATGC 1 cut(s) 1405
Sse9I AATT 4 cut(s) 470, 838, 1062, 1612
SsiI CCGC 5 cut(s) 237, 271, 360, 1405, 1429
SspI AATATT 1 cut(s) 966
SspMI CTAG 5 cut(s) 488, 726, 1158, 1370, 1623
SstI GAGCTC 1 cut(s) 25
StyD4I CCNGG 2 cut(s) 57, 1439
StyI CCWWGG 2 cut(s) 481, 942
TaaI ACNGT 4 cut(s) 46, 739, 1228, 1603
TaiI ACGT 1 cut(s) 231
TaqI TCGA 3 cut(s) 586, 992, 1482
TaqII GACCGA 1 cut(s) 1468
TasI AATT 4 cut(s) 470, 838, 1062, 1612
TfiI GAWTC 3 cut(s) 15, 1056, 1096
Tru1I TTAA 3 cut(s) 90, 611, 1586
Tru9I TTAA 3 cut(s) 90, 611, 1586
TscAI CASTG 2 cut(s) 169, 1056
TseFI GTSAC 1 cut(s) 1253
TseI GCWGC 6 cut(s) 250, 268, 383, 752, 1151, 1154
Tsp45I GTSAC 1 cut(s) 1253
TspDTI ATGAA 5 cut(s) 483, 627, 859, 1092, 1455
TspRI CASTG 2 cut(s) 169, 1056
Tth111I GACNNNGTC 1 cut(s) 46
VpaK11BI GGWCC 2 cut(s) 362, 788
XapI RAATTY 2 cut(s) 470, 1612
XceI RCATGY 3 cut(s) 509, 702, 1405
XcmI CCANNNNNNNNNTGG 1 cut(s) 812
XmnI GAANNNNTTC 1 cut(s) 1616
XspI CTAG 5 cut(s) 488, 726, 1158, 1370, 1623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.