AAA domain
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G57590 |
| fragaria_vesca | FvH4_4g09130 FvH4_4g09130 |
| malus_domestica | MD13G1267300.v1.1 |
| prunus_persica | Prupe.1G089300_v2.0.a1 Prupe.1G089300_v2.0.a1 Prupe.1G089300_v2.0.a1 |
| pyrus_communis | pycom13g22810 |
| rosa_chinensis | RchiOBHm_Chr4g0403051 |
| rosa_laevigata | RLG00000009028 |
| rosa_multiflora | Rmu_co8501967.1_g000001 Rmu_sc0001023.1_g000026 |
| rosa_roxburghii | Rroxscaffold_5G00347740 |
| rosa_rugosa | Rorug04G0040400 |
| rosa_samantha | Rh4AG117700 Rh4BG110200 Rh4CG124500 Rh4DG109600 |
| rosa_wichuraiana | Rw4G009420 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 2131 |
| Acc36I | ACCTGC | 1 cut(s) | 1288 |
| AccB1I | GGYRCC | 1 cut(s) | 862 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1661, 2154 |
| AccI | GTMKAC | 2 cut(s) | 1341, 1559 |
| AccII | CGCG | 1 cut(s) | 524 |
| AclI | AACGTT | 1 cut(s) | 2403 |
| AclWI | GGATC | 9 cut(s) | 1184, 1353, 1366, 1577, 1590, 1605, 1618, 1841, 2159 |
| AcoI | YGGCCR | 1 cut(s) | 1037 |
| AcsI | RAATTY | 6 cut(s) | 415, 708, 1105, 1893, 2442, 2486 |
| AcuI | CTGAAG | 1 cut(s) | 885 |
| AcyI | GRCGYC | 1 cut(s) | 1933 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 1535, 1661, 2154, 2325 |
| AjnI | CCWGG | 4 cut(s) | 652, 703, 1200, 1613 |
| Alw21I | GWGCWC | 3 cut(s) | 410, 448, 1798 |
| Alw26I | GTCTC | 7 cut(s) | 41, 163, 623, 1698, 1940, 2165, 2355 |
| AlwI | GGATC | 9 cut(s) | 1184, 1353, 1366, 1577, 1590, 1605, 1618, 1841, 2159 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2048 |
| Ama87I | CYCGRG | 1 cut(s) | 1335 |
| AoxI | GGCC | 7 cut(s) | 689, 817, 1037, 1184, 1734, 2390, 2422 |
| ApeKI | GCWGC | 6 cut(s) | 485, 506, 755, 794, 1235, 1549 |
| ApoI | RAATTY | 6 cut(s) | 415, 708, 1105, 1893, 2442, 2486 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 141, 173 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1729 |
| AspLEI | GCGC | 2 cut(s) | 1295, 2132 |
| AspS9I | GGNCC | 4 cut(s) | 656, 895, 1184, 2423 |
| AsuC2I | CCSGG | 2 cut(s) | 422, 2389 |
| AsuHPI | GGTGA | 4 cut(s) | 1112, 1518, 1663, 1882 |
| AsuNHI | GCTAGC | 1 cut(s) | 1015 |
| AvaI | CYCGRG | 1 cut(s) | 1335 |
| AvaII | GGWCC | 2 cut(s) | 656, 895 |
| BalI | TGGCCA | 1 cut(s) | 1039 |
| BamHI | GGATCC | 3 cut(s) | 1358, 1582, 1610 |
| BanI | GGYRCC | 1 cut(s) | 862 |
| BbsI | GAAGAC | 2 cut(s) | 268, 2379 |
| Bbv12I | GWGCWC | 3 cut(s) | 410, 448, 1798 |
| BbvI | GCAGC | 6 cut(s) | 493, 497, 767, 781, 1222, 1561 |
| BccI | CCATC | 7 cut(s) | 574, 716, 929, 1304, 1381, 1531, 2156 |
| BceAI | ACGGC | 2 cut(s) | 1537, 1662 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 136, 170 |
| BciT130I | CCWGG | 4 cut(s) | 654, 705, 1202, 1615 |
| BcnI | CCSGG | 2 cut(s) | 422, 2389 |
| BcoDI | GTCTC | 7 cut(s) | 41, 163, 623, 1698, 1940, 2165, 2355 |
| BfaI | CTAG | 5 cut(s) | 648, 1016, 1215, 1239, 2297 |
| BfmI | CTRYAG | 3 cut(s) | 162, 685, 1431 |
| BfuAI | ACCTGC | 1 cut(s) | 1288 |
| BlpI | GCTNAGC | 3 cut(s) | 1011, 1206, 1296 |
| Bme1390I | CCNGG | 6 cut(s) | 422, 654, 705, 1202, 1615, 2389 |
| Bme18I | GGWCC | 2 cut(s) | 656, 895 |
| BmeT110I | CYCGRG | 1 cut(s) | 1335 |
| BmgT120I | GGNCC | 4 cut(s) | 656, 895, 1184, 2423 |
| BmiI | GGNNCC | 9 cut(s) | 864, 897, 1185, 1360, 1584, 1612, 1666, 2019, 2284 |
| BmrFI | CCNGG | 6 cut(s) | 422, 654, 705, 1202, 1615, 2389 |
| BmsI | GCATC | 6 cut(s) | 177, 1072, 1147, 1718, 2035, 2302 |
| BmtI | GCTAGC | 1 cut(s) | 1019 |
| BpiI | GAAGAC | 2 cut(s) | 268, 2379 |
| BpmI | CTGGAG | 5 cut(s) | 173, 462, 1529, 1788, 2088 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 1011, 1206, 1296 |
| BpuMI | CCSGG | 2 cut(s) | 422, 2389 |
| BsaBI | GATNNNNATC | 1 cut(s) | 67 |
| BsaHI | GRCGYC | 1 cut(s) | 1933 |
| BsaJI | CCNNGG | 3 cut(s) | 891, 1486, 1553 |
| BsaWI | WCCGGW | 2 cut(s) | 448, 1975 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 1535, 1661, 2154, 2325 |
| Bse118I | RCCGGY | 4 cut(s) | 242, 635, 865, 2451 |
| Bse1I | ACTGG | 6 cut(s) | 445, 1567, 1811, 1898, 2011, 2235 |
| Bse3DI | GCAATG | 1 cut(s) | 1512 |
| Bse8I | GATNNNNATC | 1 cut(s) | 67 |
| BseBI | CCWGG | 4 cut(s) | 654, 705, 1202, 1615 |
| BseDI | CCNNGG | 3 cut(s) | 891, 1486, 1553 |
| BseGI | GGATG | 3 cut(s) | 408, 430, 2148 |
| BseJI | GATNNNNATC | 1 cut(s) | 67 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 1535, 1661, 2154, 2325 |
| BseMI | GCAATG | 1 cut(s) | 1512 |
| BseMII | CTCAG | 6 cut(s) | 756, 1061, 1197, 1254, 1287, 2124 |
| BseNI | ACTGG | 6 cut(s) | 445, 1567, 1811, 1898, 2011, 2235 |
| BseRI | GAGGAG | 2 cut(s) | 176, 209 |
| BseXI | GCAGC | 6 cut(s) | 493, 497, 767, 781, 1222, 1561 |
| BseYI | CCCAGC | 2 cut(s) | 1326, 1826 |
| Bsh1236I | CGCG | 1 cut(s) | 524 |
| Bsh1285I | CGRYCG | 1 cut(s) | 531 |
| BshFI | GGCC | 7 cut(s) | 691, 819, 1039, 1186, 1736, 2392, 2424 |
| BshNI | GGYRCC | 1 cut(s) | 862 |
| BsiEI | CGRYCG | 1 cut(s) | 531 |
| BsiHKAI | GWGCWC | 3 cut(s) | 410, 448, 1798 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1335 |
| BslFI | GGGAC | 2 cut(s) | 760, 2234 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 1535, 1661, 2154, 2325 |
| BsmAI | GTCTC | 7 cut(s) | 41, 163, 623, 1698, 1940, 2165, 2355 |
| BsmBI | CGTCTC | 3 cut(s) | 41, 163, 1698 |
| BsmFI | GGGAC | 2 cut(s) | 760, 2234 |
| BsmI | GAATGC | 3 cut(s) | 57, 1762, 1839 |
| BsnI | GGCC | 7 cut(s) | 691, 819, 1039, 1186, 1736, 2392, 2424 |
| BsoBI | CYCGRG | 1 cut(s) | 1335 |
| Bsp1286I | GDGCHC | 3 cut(s) | 410, 448, 1798 |
| Bsp143I | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 1011, 1206, 1296 |
| Bsp19I | CCATGG | 1 cut(s) | 1486 |
| BspANI | GGCC | 7 cut(s) | 691, 819, 1039, 1186, 1736, 2392, 2424 |
| BspCNI | CTCAG | 6 cut(s) | 755, 1060, 1198, 1255, 1288, 2123 |
| BspFNI | CGCG | 1 cut(s) | 524 |
| BspLI | GGNNCC | 9 cut(s) | 864, 897, 1185, 1360, 1584, 1612, 1666, 2019, 2284 |
| BspMI | ACCTGC | 1 cut(s) | 1288 |
| BspOI | GCTAGC | 1 cut(s) | 1019 |
| BspPI | GGATC | 9 cut(s) | 1184, 1353, 1366, 1577, 1590, 1605, 1618, 1841, 2159 |
| BspQI | GCTCTTC | 1 cut(s) | 1476 |
| BspT107I | GGYRCC | 1 cut(s) | 862 |
| BsrDI | GCAATG | 1 cut(s) | 1512 |
| BsrFI | RCCGGY | 4 cut(s) | 242, 635, 865, 2451 |
| BsrI | ACTGG | 6 cut(s) | 445, 1567, 1811, 1898, 2011, 2235 |
| BssAI | RCCGGY | 4 cut(s) | 242, 635, 865, 2451 |
| BssECI | CCNNGG | 3 cut(s) | 891, 1486, 1553 |
| BssMI | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| BssNAI | GTATAC | 2 cut(s) | 1342, 1560 |
| BssNI | GRCGYC | 1 cut(s) | 1933 |
| BssT1I | CCWWGG | 2 cut(s) | 891, 1486 |
| Bst1107I | GTATAC | 2 cut(s) | 1342, 1560 |
| Bst2UI | CCWGG | 4 cut(s) | 654, 705, 1202, 1615 |
| Bst4CI | ACNGT | 8 cut(s) | 35, 436, 529, 1075, 1081, 1136, 1594, 2378 |
| Bst6I | CTCTTC | 6 cut(s) | 570, 675, 1431, 1476, 1561, 2358 |
| BstACI | GRCGYC | 1 cut(s) | 1933 |
| BstC8I | GCNNGC | 5 cut(s) | 1017, 1148, 1168, 1212, 1987 |
| BstDSI | CCRYGG | 2 cut(s) | 1486, 1553 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1533 |
| BstF5I | GGATG | 3 cut(s) | 408, 430, 2148 |
| BstFNI | CGCG | 1 cut(s) | 524 |
| BstHHI | GCGC | 2 cut(s) | 1295, 2132 |
| BstKTI | GATC | 9 cut(s) | 814, 910, 1192, 1361, 1414, 1585, 1613, 1849, 2167 |
| BstMAI | GTCTC | 7 cut(s) | 41, 163, 623, 1698, 1940, 2165, 2355 |
| BstMBI | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| BstMCI | CGRYCG | 1 cut(s) | 531 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 482, 1016, 1211, 1511, 1991, 2045, 2138, 2144, 2464 |
| BstNI | CCWGG | 4 cut(s) | 654, 705, 1202, 1615 |
| BstNSI | RCATGY | 1 cut(s) | 1253 |
| BstSCI | CCNGG | 6 cut(s) | 420, 652, 703, 1200, 1613, 2387 |
| BstSFI | CTRYAG | 3 cut(s) | 162, 685, 1431 |
| BstUI | CGCG | 1 cut(s) | 524 |
| BstV1I | GCAGC | 6 cut(s) | 493, 497, 767, 781, 1222, 1561 |
| BstV2I | GAAGAC | 2 cut(s) | 268, 2379 |
| BstX2I | RGATCY | 5 cut(s) | 1189, 1358, 1582, 1610, 2164 |
| BstYI | RGATCY | 5 cut(s) | 1189, 1358, 1582, 1610, 2164 |
| BstZ17I | GTATAC | 2 cut(s) | 1342, 1560 |
| BsuRI | GGCC | 7 cut(s) | 691, 819, 1039, 1186, 1736, 2392, 2424 |
| BtgI | CCRYGG | 2 cut(s) | 1486, 1553 |
| BtgZI | GCGATG | 1 cut(s) | 154 |
| BtsCI | GGATG | 3 cut(s) | 408, 430, 2148 |
| BtsI | GCAGTG | 1 cut(s) | 1230 |
| BtsIMutI | CAGTG | 3 cut(s) | 438, 1230, 1796 |
| BveI | ACCTGC | 1 cut(s) | 1288 |
| Cac8I | GCNNGC | 5 cut(s) | 1017, 1148, 1168, 1212, 1987 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2048 |
| CfoI | GCGC | 2 cut(s) | 1295, 2132 |
| Cfr10I | RCCGGY | 4 cut(s) | 242, 635, 865, 2451 |
| Cfr13I | GGNCC | 4 cut(s) | 656, 895, 1184, 2423 |
| CseI | GACGC | 2 cut(s) | 1715, 1922 |
| DpnI | GATC | 9 cut(s) | 813, 909, 1191, 1360, 1413, 1584, 1612, 1848, 2166 |
| DpnII | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| DraI | TTTAAA | 1 cut(s) | 771 |
| EaeI | YGGCCR | 1 cut(s) | 1037 |
| Eam1104I | CTCTTC | 6 cut(s) | 570, 675, 1431, 1476, 1561, 2358 |
| EarI | CTCTTC | 6 cut(s) | 570, 675, 1431, 1476, 1561, 2358 |
| EciI | GGCGGA | 3 cut(s) | 17, 196, 870 |
| Eco130I | CCWWGG | 2 cut(s) | 891, 1486 |
| Eco147I | AGGCCT | 1 cut(s) | 691 |
| Eco47I | GGWCC | 2 cut(s) | 656, 895 |
| Eco57I | CTGAAG | 1 cut(s) | 885 |
| Eco88I | CYCGRG | 1 cut(s) | 1335 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1533 |
| EcoO109I | RGGNCCY | 1 cut(s) | 895 |
| EcoRI | GAATTC | 2 cut(s) | 708, 2486 |
| EcoRII | CCWGG | 4 cut(s) | 652, 703, 1200, 1613 |
| EcoT14I | CCWWGG | 2 cut(s) | 891, 1486 |
| EcoT22I | ATGCAT | 1 cut(s) | 1839 |
| ErhI | CCWWGG | 2 cut(s) | 891, 1486 |
| Esp3I | CGTCTC | 3 cut(s) | 41, 163, 1698 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 2076, 2108 |
| FaqI | GGGAC | 2 cut(s) | 760, 2234 |
| FauI | CCCGC | 1 cut(s) | 364 |
| FauNDI | CATATG | 1 cut(s) | 1839 |
| FblI | GTMKAC | 2 cut(s) | 1341, 1559 |
| FokI | GGATG | 3 cut(s) | 415, 437, 2135 |
| FspBI | CTAG | 5 cut(s) | 648, 1016, 1215, 1239, 2297 |
| FspI | TGCGCA | 1 cut(s) | 2131 |
| GlaI | GCGC | 2 cut(s) | 1294, 2131 |
| GsaI | CCCAGC | 2 cut(s) | 1330, 1830 |
| GsuI | CTGGAG | 5 cut(s) | 173, 462, 1529, 1788, 2088 |
| HaeIII | GGCC | 7 cut(s) | 691, 819, 1039, 1186, 1736, 2392, 2424 |
| HgaI | GACGC | 2 cut(s) | 1715, 1922 |
| HhaI | GCGC | 2 cut(s) | 1295, 2132 |
| Hin1I | GRCGYC | 1 cut(s) | 1933 |
| Hin6I | GCGC | 2 cut(s) | 1293, 2130 |
| HinP1I | GCGC | 2 cut(s) | 1293, 2130 |
| HincII | GTYRAC | 1 cut(s) | 2004 |
| HindII | GTYRAC | 1 cut(s) | 2004 |
| HindIII | AAGCTT | 2 cut(s) | 948, 2078 |
| HphI | GGTGA | 4 cut(s) | 1112, 1518, 1663, 1882 |
| Hpy166II | GTNNAC | 5 cut(s) | 1176, 1342, 1560, 1920, 2004 |
| Hpy8I | GTNNAC | 5 cut(s) | 1176, 1342, 1560, 1920, 2004 |
| Hpy99I | CGWCG | 4 cut(s) | 309, 529, 545, 664 |
| HpyAV | CCTTC | 5 cut(s) | 207, 261, 565, 909, 1678 |
| HpyCH4III | ACNGT | 8 cut(s) | 35, 436, 529, 1075, 1081, 1136, 1594, 2378 |
| HpyCH4IV | ACGT | 2 cut(s) | 467, 2403 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 482, 1016, 1211, 1511, 1991, 2045, 2138, 2144, 2464 |
| HpySE526I | ACGT | 2 cut(s) | 467, 2403 |
| Hsp92I | GRCGYC | 1 cut(s) | 1933 |
| HspAI | GCGC | 2 cut(s) | 1293, 2130 |
| Kzo9I | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| LguI | GCTCTTC | 1 cut(s) | 1476 |
| LmnI | GCTCC | 3 cut(s) | 443, 1502, 1793 |
| Lsp1109I | GCAGC | 6 cut(s) | 493, 497, 767, 781, 1222, 1561 |
| LweI | GCATC | 6 cut(s) | 177, 1072, 1147, 1718, 2035, 2302 |
| MaeI | CTAG | 5 cut(s) | 648, 1016, 1215, 1239, 2297 |
| MaeII | ACGT | 2 cut(s) | 467, 2403 |
| MaeIII | GTNAC | 7 cut(s) | 10, 436, 546, 1075, 1226, 2029, 2099 |
| MalI | GATC | 9 cut(s) | 813, 909, 1191, 1360, 1413, 1584, 1612, 1848, 2166 |
| MboI | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| MflI | RGATCY | 5 cut(s) | 1189, 1358, 1582, 1610, 2164 |
| MhlI | GDGCHC | 3 cut(s) | 410, 448, 1798 |
| MlsI | TGGCCA | 1 cut(s) | 1039 |
| MluNI | TGGCCA | 1 cut(s) | 1039 |
| MlyI | GAGTC | 2 cut(s) | 530, 1082 |
| MmeI | TCCRAC | 4 cut(s) | 354, 697, 1771, 1882 |
| Mox20I | TGGCCA | 1 cut(s) | 1039 |
| Mph1103I | ATGCAT | 1 cut(s) | 1839 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1729 |
| MscI | TGGCCA | 1 cut(s) | 1039 |
| MseI | TTAA | 9 cut(s) | 119, 594, 770, 825, 839, 998, 1458, 2082, 2207 |
| Msp20I | TGGCCA | 1 cut(s) | 1039 |
| MspR9I | CCNGG | 6 cut(s) | 422, 654, 705, 1202, 1615, 2389 |
| Mva1269I | GAATGC | 3 cut(s) | 57, 1762, 1839 |
| MvaI | CCWGG | 4 cut(s) | 654, 705, 1202, 1615 |
| MvnI | CGCG | 1 cut(s) | 524 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 482, 1016, 1211, 1511, 1991, 2045, 2138, 2144, 2464 |
| NciI | CCSGG | 2 cut(s) | 422, 2389 |
| NcoI | CCATGG | 1 cut(s) | 1486 |
| NdeI | CATATG | 1 cut(s) | 1839 |
| NdeII | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| NheI | GCTAGC | 1 cut(s) | 1015 |
| NlaIV | GGNNCC | 9 cut(s) | 864, 897, 1185, 1360, 1584, 1612, 1666, 2019, 2284 |
| NmeAIII | GCCGAG | 1 cut(s) | 123 |
| NmuCI | GTSAC | 4 cut(s) | 436, 1075, 2029, 2099 |
| NsbI | TGCGCA | 1 cut(s) | 2131 |
| NsiI | ATGCAT | 1 cut(s) | 1839 |
| NspI | RCATGY | 1 cut(s) | 1253 |
| PaeR7I | CTCGAG | 1 cut(s) | 1335 |
| PceI | AGGCCT | 1 cut(s) | 691 |
| PciSI | GCTCTTC | 1 cut(s) | 1476 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 528, 537 |
| PctI | GAATGC | 3 cut(s) | 57, 1762, 1839 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1729 |
| PfeI | GAWTC | 8 cut(s) | 63, 257, 263, 290, 929, 1926, 2057, 2072 |
| PflFI | GACNNNGTC | 1 cut(s) | 539 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1661, 2154 |
| PfoI | TCCNGGA | 2 cut(s) | 420, 1613 |
| PleI | GAGTC | 2 cut(s) | 530, 1082 |
| PpsI | GAGTC | 2 cut(s) | 530, 1082 |
| PpuMI | RGGWCCY | 1 cut(s) | 895 |
| Psp1406I | AACGTT | 1 cut(s) | 2403 |
| Psp5II | RGGWCCY | 1 cut(s) | 895 |
| Psp6I | CCWGG | 4 cut(s) | 652, 703, 1200, 1613 |
| PspFI | CCCAGC | 2 cut(s) | 1326, 1826 |
| PspGI | CCWGG | 4 cut(s) | 652, 703, 1200, 1613 |
| PspN4I | GGNNCC | 9 cut(s) | 864, 897, 1185, 1360, 1584, 1612, 1666, 2019, 2284 |
| PspPI | GGNCC | 4 cut(s) | 656, 895, 1184, 2423 |
| PspPPI | RGGWCCY | 1 cut(s) | 895 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2048 |
| PsuI | RGATCY | 5 cut(s) | 1189, 1358, 1582, 1610, 2164 |
| PsyI | GACNNNGTC | 1 cut(s) | 539 |
| SapI | GCTCTTC | 1 cut(s) | 1476 |
| SaqAI | TTAA | 9 cut(s) | 119, 594, 770, 825, 839, 998, 1458, 2082, 2207 |
| Sau3AI | GATC | 9 cut(s) | 811, 907, 1189, 1358, 1411, 1582, 1610, 1846, 2164 |
| Sau96I | GGNCC | 4 cut(s) | 656, 895, 1184, 2423 |
| SchI | GAGTC | 2 cut(s) | 530, 1082 |
| ScrFI | CCNGG | 6 cut(s) | 422, 654, 705, 1202, 1615, 2389 |
| SduI | GDGCHC | 3 cut(s) | 410, 448, 1798 |
| SfaNI | GCATC | 6 cut(s) | 177, 1072, 1147, 1718, 2035, 2302 |
| SfcI | CTRYAG | 3 cut(s) | 162, 685, 1431 |
| Sfr274I | CTCGAG | 1 cut(s) | 1335 |
| SinI | GGWCC | 2 cut(s) | 656, 895 |
| SlaI | CTCGAG | 1 cut(s) | 1335 |
| SmlI | CTYRAG | 1 cut(s) | 1335 |
| SmoI | CTYRAG | 1 cut(s) | 1335 |
| SseBI | AGGCCT | 1 cut(s) | 691 |
| SspMI | CTAG | 5 cut(s) | 648, 1016, 1215, 1239, 2297 |
| StuI | AGGCCT | 1 cut(s) | 691 |
| StyD4I | CCNGG | 6 cut(s) | 420, 652, 703, 1200, 1613, 2387 |
| StyI | CCWWGG | 2 cut(s) | 891, 1486 |
| TaaI | ACNGT | 8 cut(s) | 35, 436, 529, 1075, 1081, 1136, 1594, 2378 |
| TaiI | ACGT | 2 cut(s) | 470, 2406 |
| TauI | GCSGC | 5 cut(s) | 176, 362, 730, 2141, 2451 |
| TfiI | GAWTC | 8 cut(s) | 63, 257, 263, 290, 929, 1926, 2057, 2072 |
| Tru1I | TTAA | 9 cut(s) | 119, 594, 770, 825, 839, 998, 1458, 2082, 2207 |
| Tru9I | TTAA | 9 cut(s) | 119, 594, 770, 825, 839, 998, 1458, 2082, 2207 |
| TscAI | CASTG | 3 cut(s) | 445, 1237, 1803 |
| TseFI | GTSAC | 4 cut(s) | 436, 1075, 2029, 2099 |
| TseI | GCWGC | 6 cut(s) | 485, 506, 755, 794, 1235, 1549 |
| Tsp45I | GTSAC | 4 cut(s) | 436, 1075, 2029, 2099 |
| TspDTI | ATGAA | 8 cut(s) | 189, 834, 843, 1290, 1808, 1926, 2049, 2084 |
| TspGWI | ACGGA | 3 cut(s) | 293, 529, 648 |
| TspRI | CASTG | 3 cut(s) | 445, 1237, 1803 |
| Tth111I | GACNNNGTC | 1 cut(s) | 539 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1661, 2154 |
| VpaK11BI | GGWCC | 2 cut(s) | 656, 895 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1533 |
| XapI | RAATTY | 6 cut(s) | 415, 708, 1105, 1893, 2442, 2486 |
| XbaI | TCTAGA | 1 cut(s) | 2296 |
| XceI | RCATGY | 1 cut(s) | 1253 |
| XhoI | CTCGAG | 1 cut(s) | 1335 |
| XmiI | GTMKAC | 2 cut(s) | 1341, 1559 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1729 |
| XspI | CTAG | 5 cut(s) | 648, 1016, 1215, 1239, 2297 |
| Zsp2I | ATGCAT | 1 cut(s) | 1839 |