AT5G57950

regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011000
Physical Location & Seq
Forward (+)
23460673 .. 23462388
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57950.2

Sequence Viewer

Length: 684 bp
ATGGTTGGCGCGAATCTGAAAGCGGAGACGATGGCTCTAATGGACAAGAGAACAGCCATGGAGACGGAGATGAACTCTATTGTCGAACGTCTCTGCAATCCCGGTGGTCCTGGTCTCTCCGGCAATCTCATCGACTCGGAGGGATTTCCTCGGGAAGATATTGACATTCCCATGGTGAGAACTGAGCGCCGTCGTCTTGCTGAGTTAAGGAGTGAGCACGGGGAGATAACGGAAAAGATAAATGTGAACATTCAAATTCTTCATTCAGTGAGGCCTACATCTAGAGCTTCATCCACAAAAGATTCAGGTCCAGAAGAAACAAGCTTGTCAGGTGCTGCAGTAAATTCTTTATCTGCCTCCATGCAGACATCTGGCTTCAGTGTTACTTCCGGTCCCATGGATGTGGATGTGGTTACTAGTATTCCCTTTGCAATGGTTGATGAGATAAACGAGTCATCTCCAGCAGCAGAGGGTGGATTACAGCTTGGAGATCAGGTTTTGAAATTTGGCAACGTGGAGGGTGGTGACAACCTGTTGCAAAGGCTTGCCGCTGAAGCTCAGTCTAACCAGGGACAAGCCGTTTCTGTTCAAGTTATGAGGCAAGGTGCAAAAGTCGTCTTATCTGTCACACCAAGGATATGGCAAGGCAGAGGTCTATTGGGGTGCCATTTCCGTTTGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

227

Amino Acids

24.26

Weight (kDa)

5.07

Isoelectric Point (pI)

38.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nas2_N PF18265 41 - 118 8.1e-20 Nas2 N_terminal domain
PDZ_2 PF13180 172 - 238 8.6e-07 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57950 AT5G57950
fragaria_vesca FvH4_1g00940 FvH4_1g00940 FvH4_1g00940
malus_domestica MD02G1007700.v1.1 MD09G1175000.v1.1
prunus_persica Prupe.7G262800_v2.0.a1
pyrus_communis pycom02g00660 pycom15g13650
rosa_chinensis RchiOBHm_Chr2g0085671
rosa_laevigata RLG00000015710
rosa_multiflora Rmu_co8318769.1_g000001 Rmu_sc0007631.1_g000007
rosa_roxburghii Rroxscaffold_2G00155140
rosa_rugosa Rorug01G0462100
rosa_samantha Rh2AG011300 Rh2BG011800 Rh2CG012600
rosa_wichuraiana Rw2G001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 663
AccII CGCG 1 cut(s) 11
AciI CCGC 2 cut(s) 23, 549
AcsI RAATTY 3 cut(s) 255, 343, 503
AcuI CTGAAG 2 cut(s) 361, 573
AgsI TTSAA 3 cut(s) 254, 502, 590
AhlI ACTAGT 1 cut(s) 416
AjnI CCWGG 2 cut(s) 109, 567
AluBI AGCT 4 cut(s) 287, 324, 484, 557
AluI AGCT 4 cut(s) 287, 324, 484, 557
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 4 cut(s) 20, 56, 95, 119
AlwNI CAGNNNCTG 1 cut(s) 335
Ama87I CYCGRG 1 cut(s) 150
AoxI GGCC 1 cut(s) 272
ApeKI GCWGC 2 cut(s) 335, 464
ApoI RAATTY 3 cut(s) 255, 343, 503
AspLEI GCGC 2 cut(s) 11, 189
AspS9I GGNCC 3 cut(s) 107, 308, 392
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 2 cut(s) 187, 536
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 3 cut(s) 107, 308, 392
BanI GGYRCC 1 cut(s) 663
Bbv12I GWGCWC 1 cut(s) 219
BbvI GCAGC 2 cut(s) 322, 476
BccI CCATC 1 cut(s) 25
BceAI ACGGC 2 cut(s) 174, 563
BcgI CGANNNNNNTGC 2 cut(s) 112, 146
BciT130I CCWGG 2 cut(s) 111, 569
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 4 cut(s) 20, 56, 95, 119
BcuI ACTAGT 1 cut(s) 416
BfaI CTAG 2 cut(s) 282, 417
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 190
BisI GCNGC 3 cut(s) 336, 465, 549
BlsI GCNGC 3 cut(s) 337, 466, 550
Bme1390I CCNGG 3 cut(s) 102, 111, 569
Bme18I GGWCC 3 cut(s) 107, 308, 392
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 3 cut(s) 107, 308, 392
BmiI GGNNCC 2 cut(s) 394, 665
BmrFI CCNGG 3 cut(s) 102, 111, 569
BplI GAGNNNNNCTC 2 cut(s) 59, 91
BpmI CTGGAG 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 102
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 6 cut(s) 57, 149, 171, 396, 568, 632
BsaWI WCCGGW 1 cut(s) 389
BsaXI ACNNNNNCTCC 2 cut(s) 480, 510
Bse3DI GCAATG 1 cut(s) 438
BseBI CCWGG 2 cut(s) 111, 569
BseDI CCNNGG 6 cut(s) 57, 149, 171, 396, 568, 632
BseGI GGATG 3 cut(s) 290, 406, 412
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 3 cut(s) 174, 192, 572
BseXI GCAGC 2 cut(s) 322, 476
Bsh1236I CGCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 274
BshNI GGYRCC 1 cut(s) 663
BsiHKAI GWGCWC 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 3 cut(s) 102, 120, 390
BslFI GGGAC 2 cut(s) 378, 585
BsmAI GTCTC 4 cut(s) 20, 56, 95, 119
BsmBI CGTCTC 3 cut(s) 20, 56, 95
BsmFI GGGAC 2 cut(s) 378, 585
BsnI GGCC 1 cut(s) 274
Bso31I GGTCTC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 150
Bsp1286I GDGCHC 1 cut(s) 219
Bsp143I GATC 1 cut(s) 490
Bsp19I CCATGG 3 cut(s) 57, 171, 396
BspACI CCGC 2 cut(s) 23, 549
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 3 cut(s) 175, 193, 571
BspFNI CGCG 1 cut(s) 11
BspLI GGNNCC 2 cut(s) 394, 665
BspMAI CTGCAG 1 cut(s) 340
BspT107I GGYRCC 1 cut(s) 663
BspTNI GGTCTC 1 cut(s) 119
BsrDI GCAATG 1 cut(s) 438
BssECI CCNNGG 6 cut(s) 57, 149, 171, 396, 568, 632
BssMI GATC 1 cut(s) 490
BssT1I CCWWGG 4 cut(s) 57, 171, 396, 632
Bst2UI CCWGG 2 cut(s) 111, 569
BstC8I GCNNGC 1 cut(s) 546
BstDEI CTNAG 3 cut(s) 183, 201, 558
BstDSI CCRYGG 3 cut(s) 57, 171, 396
BstF5I GGATG 3 cut(s) 290, 406, 412
BstFNI CGCG 1 cut(s) 11
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 2 cut(s) 11, 189
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 4 cut(s) 20, 56, 95, 119
BstMBI GATC 1 cut(s) 490
BstMWI GCNNNNNNNGC 1 cut(s) 554
BstNI CCWGG 2 cut(s) 111, 569
BstSCI CCNGG 3 cut(s) 100, 109, 567
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 1 cut(s) 11
BstV1I GCAGC 2 cut(s) 322, 476
BstXI CCANNNNNNTGG 2 cut(s) 403, 639
BsuRI GGCC 1 cut(s) 274
BtgI CCRYGG 3 cut(s) 57, 171, 396
BtsCI GGATG 3 cut(s) 290, 406, 412
BtsIMutI CAGTG 2 cut(s) 273, 385
Cac8I GCNNGC 1 cut(s) 546
CaiI CAGNNNCTG 1 cut(s) 335
CfoI GCGC 2 cut(s) 11, 189
Cfr13I GGNCC 3 cut(s) 107, 308, 392
CspCI CAANNNNNGTGG 2 cut(s) 85, 120
CviAII CATG 4 cut(s) 58, 172, 361, 397
DdeI CTNAG 3 cut(s) 183, 201, 558
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
Eco130I CCWWGG 4 cut(s) 57, 171, 396, 632
Eco147I AGGCCT 1 cut(s) 274
Eco31I GGTCTC 1 cut(s) 119
Eco47I GGWCC 3 cut(s) 107, 308, 392
Eco57I CTGAAG 2 cut(s) 361, 573
Eco88I CYCGRG 1 cut(s) 150
EcoRII CCWGG 2 cut(s) 109, 567
EcoT14I CCWWGG 4 cut(s) 57, 171, 396, 632
ErhI CCWWGG 4 cut(s) 57, 171, 396, 632
Esp3I CGTCTC 3 cut(s) 20, 56, 95
FaeI CATG 4 cut(s) 61, 175, 364, 400
FaiI YATR 7 cut(s) 59, 173, 362, 398, 596, 640, 682
FaqI GGGAC 2 cut(s) 378, 585
FatI CATG 4 cut(s) 57, 171, 360, 396
Fnu4HI GCNGC 3 cut(s) 336, 465, 549
FokI GGATG 3 cut(s) 277, 413, 419
Fsp4HI GCNGC 3 cut(s) 336, 465, 549
FspBI CTAG 2 cut(s) 282, 417
GlaI GCGC 2 cut(s) 10, 188
GluI GCNGC 3 cut(s) 336, 465, 549
GsuI CTGGAG 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 1 cut(s) 274
HapII CCGG 3 cut(s) 102, 120, 390
HhaI GCGC 2 cut(s) 11, 189
Hin1II CATG 4 cut(s) 61, 175, 364, 400
Hin6I GCGC 2 cut(s) 9, 187
HinP1I GCGC 2 cut(s) 9, 187
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 4 cut(s) 13, 134, 302, 452
HpaII CCGG 3 cut(s) 102, 120, 390
HphI GGTGA 2 cut(s) 187, 536
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 2 cut(s) 18, 139
Hpy188III TCNNGA 3 cut(s) 152, 282, 311
Hpy8I GTNNAC 1 cut(s) 247
Hpy99I CGWCG 1 cut(s) 195
HpyCH4IV ACGT 2 cut(s) 88, 513
HpyCH4V TGCA 6 cut(s) 96, 338, 364, 431, 538, 608
HpyF10VI GCNNNNNNNGC 1 cut(s) 554
HpyF3I CTNAG 3 cut(s) 183, 201, 558
HpySE526I ACGT 2 cut(s) 88, 513
Hsp92II CATG 4 cut(s) 61, 175, 364, 400
HspAI GCGC 2 cut(s) 9, 187
Kzo9I GATC 1 cut(s) 490
Lsp1109I GCAGC 2 cut(s) 322, 476
MaeI CTAG 2 cut(s) 282, 417
MaeII ACGT 2 cut(s) 88, 513
MaeIII GTNAC 4 cut(s) 382, 412, 524, 625
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 3 cut(s) 167, 251, 326
MhlI GDGCHC 1 cut(s) 219
MluCI AATT 3 cut(s) 255, 343, 503
MlyI GAGTC 2 cut(s) 128, 461
MnlI CCTC 8 cut(s) 133, 159, 264, 367, 463, 511, 591, 644
MseI TTAA 1 cut(s) 206
MslI CAYNNNNRTG 2 cut(s) 170, 401
MspA1I CMGCKG 1 cut(s) 551
MspI CCGG 3 cut(s) 102, 120, 390
MspR9I CCNGG 3 cut(s) 102, 111, 569
MvaI CCWGG 2 cut(s) 111, 569
MvnI CGCG 1 cut(s) 11
MwoI GCNNNNNNNGC 1 cut(s) 554
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 3 cut(s) 57, 171, 396
NdeII GATC 1 cut(s) 490
NlaIII CATG 4 cut(s) 61, 175, 364, 400
NlaIV GGNNCC 2 cut(s) 394, 665
NmuCI GTSAC 2 cut(s) 524, 625
PceI AGGCCT 1 cut(s) 274
PfeI GAWTC 2 cut(s) 13, 302
PkrI GCNGC 3 cut(s) 337, 466, 550
PleI GAGTC 2 cut(s) 128, 460
PpsI GAGTC 2 cut(s) 128, 460
Psp6I CCWGG 2 cut(s) 109, 567
PspGI CCWGG 2 cut(s) 109, 567
PspN4I GGNNCC 2 cut(s) 394, 665
PspPI GGNCC 3 cut(s) 107, 308, 392
PstI CTGCAG 1 cut(s) 340
PstNI CAGNNNCTG 1 cut(s) 335
RseI CAYNNNNRTG 2 cut(s) 170, 401
SaqAI TTAA 1 cut(s) 206
SatI GCNGC 3 cut(s) 336, 465, 549
Sau3AI GATC 1 cut(s) 490
Sau96I GGNCC 3 cut(s) 107, 308, 392
SchI GAGTC 2 cut(s) 128, 461
ScrFI CCNGG 3 cut(s) 102, 111, 569
SduI GDGCHC 1 cut(s) 219
SfcI CTRYAG 1 cut(s) 336
SinI GGWCC 3 cut(s) 107, 308, 392
SmiMI CAYNNNNRTG 2 cut(s) 170, 401
SpeI ACTAGT 1 cut(s) 416
Sse9I AATT 3 cut(s) 255, 343, 503
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 2 cut(s) 23, 549
SspMI CTAG 2 cut(s) 282, 417
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 3 cut(s) 100, 109, 567
StyI CCWWGG 4 cut(s) 57, 171, 396, 632
TaiI ACGT 2 cut(s) 91, 516
TaqI TCGA 2 cut(s) 84, 132
TasI AATT 3 cut(s) 255, 343, 503
TauI GCSGC 1 cut(s) 551
TfiI GAWTC 2 cut(s) 13, 302
Tru1I TTAA 1 cut(s) 206
Tru9I TTAA 1 cut(s) 206
TscAI CASTG 2 cut(s) 273, 385
TseFI GTSAC 2 cut(s) 524, 625
TseI GCWGC 2 cut(s) 335, 464
Tsp45I GTSAC 2 cut(s) 524, 625
TspDTI ATGAA 3 cut(s) 86, 251, 279
TspGWI ACGGA 3 cut(s) 80, 245, 662
TspRI CASTG 2 cut(s) 273, 385
VpaK11BI GGWCC 3 cut(s) 107, 308, 392
XapI RAATTY 3 cut(s) 255, 343, 503
XbaI TCTAGA 1 cut(s) 281
XspI CTAG 2 cut(s) 282, 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.