Rroxscaffold_2G00155140

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011000
Physical Location & Seq
Reverse (-)
91527445 .. 91529531
2087 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155140.1

Sequence Viewer

Length: 657 bp
ATGGTGGCGGCGAATCTGAAGGCGGAGACTTTGGGTCTGATGGAGAAGAGGAGTGCTTTGGAGGCGGAGATGAATGCCATCATCGGGCGTCTTACTCAGCCCGGCGGTCCTGGACTTTCCGGCAACCTCCTTAACTTCGAGGGGTTTCCTCGCGAGGACATTGACATCCCAGCTGTGCGAGCTGAACGGCGTCGTCTTGCTGAACTGAAAAATGATTACAAAGAGATTACGGAGAAATTGGACCGAAATATACAAGTTTTGCATTCGGCAAAGCTTGCTCCAAAATCATCATCCCTCAAACCATCAGATGGTCAGAGTGGTTCTGTTGTCAATGTTGGAGAATCGGCATCTTCAACCAACGTTTCTTCAAATGCTATGGATGTGGATGTGAGCGTACCTTTTGCATTGGTTGATGAGATAGCTGATGCATCACCAGCAGCAGAGGATGGTTTACAGCTTGGAGATCAGATTGTGAAATTTGGCAATGTAGAAAATGGTGACAATTTGTTGCAGAAACTTGCTTCTGAGGCTCAAACAAATCAGGGTTGTGCTGTACCTGTTATGGTCTTAAGGCAAGGTGCCCAAGTCAACTTAATGGTGACACCTAGAACATGGCAAGGCAGGGGTCTACTGGGGTGTCATTTCCAGATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

218

Amino Acids

23.26

Weight (kDa)

5.04

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nas2_N PF18265 13 - 89 3.7e-21 Nas2 N_terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57950 AT5G57950
fragaria_vesca FvH4_1g00940 FvH4_1g00940 FvH4_1g00940
malus_domestica MD02G1007700.v1.1 MD09G1175000.v1.1
prunus_persica Prupe.7G262800_v2.0.a1
pyrus_communis pycom02g00660 pycom15g13650
rosa_chinensis RchiOBHm_Chr2g0085671
rosa_laevigata RLG00000015710
rosa_multiflora Rmu_co8318769.1_g000001 Rmu_sc0007631.1_g000007
rosa_roxburghii Rroxscaffold_2G00155140
rosa_rugosa Rorug01G0462100
rosa_samantha Rh2AG011300 Rh2BG011800 Rh2CG012600
rosa_wichuraiana Rw2G001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 578
AccB7I CCANNNNNTGG 1 cut(s) 308
AccI GTMKAC 1 cut(s) 628
AccII CGCG 1 cut(s) 153
AciI CCGC 4 cut(s) 8, 23, 65, 105
AclI AACGTT 1 cut(s) 360
AcsI RAATTY 1 cut(s) 476
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 2 cut(s) 88, 190
AfaI GTAC 2 cut(s) 396, 555
AfiI CCNNNNNNNGG 2 cut(s) 84, 308
AflII CTTAAG 1 cut(s) 568
AgsI TTSAA 2 cut(s) 354, 369
AhdI GACNNNNNGTC 1 cut(s) 33
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 173, 182, 274, 422, 457
AluI AGCT 5 cut(s) 173, 182, 274, 422, 457
Alw26I GTCTC 1 cut(s) 20
ApeKI GCWGC 1 cut(s) 437
ApoI RAATTY 1 cut(s) 476
AspS9I GGNCC 2 cut(s) 107, 241
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 3 cut(s) 423, 509, 610
AvaII GGWCC 2 cut(s) 107, 241
BaeGI GKGCMC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 578
BbvI GCAGC 1 cut(s) 449
BccI CCATC 5 cut(s) 34, 86, 302, 310, 440
BceAI ACGGC 1 cut(s) 203
BciT130I CCWGG 1 cut(s) 111
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 606
BfrI CTTAAG 1 cut(s) 568
BglII AGATCT 1 cut(s) 648
BisI GCNGC 2 cut(s) 9, 438
BlsI GCNGC 2 cut(s) 10, 439
Bme1390I CCNGG 2 cut(s) 102, 111
Bme18I GGWCC 2 cut(s) 107, 241
BmeRI GACNNNNNGTC 1 cut(s) 33
BmgT120I GGNCC 2 cut(s) 107, 241
BmiI GGNNCC 1 cut(s) 580
BmrFI CCNGG 2 cut(s) 102, 111
BmrI ACTGGG 1 cut(s) 641
BmsI GCATC 3 cut(s) 356, 415, 437
BmuI ACTGGG 1 cut(s) 641
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 2 cut(s) 88, 190
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 308
Bse1I ACTGG 1 cut(s) 636
Bse3DI GCAATG 1 cut(s) 490
BseBI CCWGG 1 cut(s) 111
BseGI GGATG 5 cut(s) 165, 290, 385, 391, 451
BseLI CCNNNNNNNGG 2 cut(s) 84, 308
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 2 cut(s) 110, 516
BseNI ACTGG 1 cut(s) 636
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 583
BseXI GCAGC 1 cut(s) 449
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 153
BshNI GGYRCC 1 cut(s) 578
BsiSI CCGG 2 cut(s) 102, 120
BslI CCNNNNNNNGG 2 cut(s) 84, 308
BsmAI GTCTC 1 cut(s) 20
BsmI GAATGC 2 cut(s) 79, 262
Bsp1286I GDGCHC 1 cut(s) 583
Bsp143I GATC 2 cut(s) 463, 648
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 4 cut(s) 8, 23, 65, 105
BspCNI CTCAG 2 cut(s) 109, 517
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 580
BspT107I GGYRCC 1 cut(s) 578
BspTI CTTAAG 1 cut(s) 568
BsrDI GCAATG 1 cut(s) 490
BsrI ACTGG 1 cut(s) 636
BssMI GATC 2 cut(s) 463, 648
BssNI GRCGYC 2 cut(s) 88, 190
Bst2UI CCWGG 1 cut(s) 111
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 2 cut(s) 88, 190
BstAFI CTTAAG 1 cut(s) 568
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 2 cut(s) 180, 276
BstDEI CTNAG 2 cut(s) 96, 525
BstF5I GGATG 5 cut(s) 165, 290, 385, 391, 451
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 2 cut(s) 466, 651
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 2 cut(s) 463, 648
BstMWI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 434, 527
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 100, 109
BstSLI GKGCMC 1 cut(s) 583
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 449
BstX2I RGATCY 1 cut(s) 648
BstYI RGATCY 1 cut(s) 648
BtsCI GGATG 5 cut(s) 165, 290, 385, 391, 451
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 180, 276
Cfr13I GGNCC 2 cut(s) 107, 241
CseI GACGC 2 cut(s) 77, 179
Csp6I GTAC 2 cut(s) 395, 554
CviAII CATG 1 cut(s) 612
CviJI RGCY 7 cut(s) 100, 173, 182, 274, 422, 457, 530
CviKI_1 RGCY 7 cut(s) 100, 173, 182, 274, 422, 457, 530
CviQI GTAC 2 cut(s) 395, 554
DdeI CTNAG 2 cut(s) 96, 525
DpnI GATC 2 cut(s) 465, 650
DpnII GATC 2 cut(s) 463, 648
DriI GACNNNNNGTC 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 41
Eam1105I GACNNNNNGTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 2 cut(s) 38, 80
Eco47I GGWCC 2 cut(s) 107, 241
Eco57I CTGAAG 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 430
FaeI CATG 1 cut(s) 615
FaiI YATR 4 cut(s) 251, 377, 563, 613
FatI CATG 1 cut(s) 611
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 2 cut(s) 9, 438
FokI GGATG 5 cut(s) 152, 277, 392, 398, 458
Fsp4HI GCNGC 2 cut(s) 9, 438
FspBI CTAG 1 cut(s) 606
GluI GCNGC 2 cut(s) 9, 438
GsaI CCCAGC 1 cut(s) 173
HapII CCGG 2 cut(s) 102, 120
HgaI GACGC 2 cut(s) 77, 179
Hin1I GRCGYC 2 cut(s) 88, 190
Hin1II CATG 1 cut(s) 615
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 13, 341
HpaII CCGG 2 cut(s) 102, 120
HphI GGTGA 3 cut(s) 423, 509, 610
Hpy166II GTNNAC 3 cut(s) 452, 589, 629
Hpy188I TCNGA 6 cut(s) 18, 39, 307, 315, 468, 526
Hpy188III TCNNGA 2 cut(s) 152, 646
Hpy8I GTNNAC 3 cut(s) 452, 589, 629
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 4 cut(s) 262, 404, 428, 511
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 434, 527
HpyF3I CTNAG 2 cut(s) 96, 525
HpySE526I ACGT 1 cut(s) 360
Hsp92I GRCGYC 2 cut(s) 88, 190
Hsp92II CATG 1 cut(s) 615
Kzo9I GATC 2 cut(s) 463, 648
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 1 cut(s) 449
LweI GCATC 3 cut(s) 356, 415, 437
MaeI CTAG 1 cut(s) 606
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 2 cut(s) 497, 598
MalI GATC 2 cut(s) 465, 650
MboI GATC 2 cut(s) 463, 648
MboII GAAGA 3 cut(s) 58, 342, 357
MflI RGATCY 1 cut(s) 648
MhlI GDGCHC 1 cut(s) 583
MluCI AATT 3 cut(s) 236, 476, 502
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 9 cut(s) 42, 55, 133, 137, 148, 159, 305, 436, 520
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 3 cut(s) 132, 569, 593
MspA1I CMGCKG 1 cut(s) 173
MspCI CTTAAG 1 cut(s) 568
MspI CCGG 2 cut(s) 102, 120
MspR9I CCNGG 2 cut(s) 102, 111
Mva1269I GAATGC 2 cut(s) 79, 262
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 434, 527
NciI CCSGG 1 cut(s) 102
NdeII GATC 2 cut(s) 463, 648
NlaIII CATG 1 cut(s) 615
NlaIV GGNNCC 1 cut(s) 580
NmuCI GTSAC 2 cut(s) 497, 598
NruI TCGCGA 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 430
PctI GAATGC 2 cut(s) 79, 262
PfeI GAWTC 2 cut(s) 13, 341
PflMI CCANNNNNTGG 1 cut(s) 308
PfoI TCCNGGA 1 cut(s) 109
PkrI GCNGC 2 cut(s) 10, 439
Psp1406I AACGTT 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 580
PspPI GGNCC 2 cut(s) 107, 241
PsuI RGATCY 1 cut(s) 648
PvuII CAGCTG 1 cut(s) 173
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 2 cut(s) 396, 555
RsaNI GTAC 2 cut(s) 395, 554
SaqAI TTAA 3 cut(s) 132, 569, 593
SatI GCNGC 2 cut(s) 9, 438
Sau3AI GATC 2 cut(s) 463, 648
Sau96I GGNCC 2 cut(s) 107, 241
ScrFI CCNGG 2 cut(s) 102, 111
SduI GDGCHC 1 cut(s) 583
SfaNI GCATC 3 cut(s) 356, 415, 437
SinI GGWCC 2 cut(s) 107, 241
SmlI CTYRAG 1 cut(s) 568
SmoI CTYRAG 1 cut(s) 568
Sse9I AATT 3 cut(s) 236, 476, 502
SsiI CCGC 4 cut(s) 8, 23, 65, 105
SspMI CTAG 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 100, 109
TaiI ACGT 1 cut(s) 363
TaqI TCGA 1 cut(s) 138
TaqII GACCGA 1 cut(s) 258
TasI AATT 3 cut(s) 236, 476, 502
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 2 cut(s) 13, 341
Tru1I TTAA 3 cut(s) 132, 569, 593
Tru9I TTAA 3 cut(s) 132, 569, 593
TseFI GTSAC 2 cut(s) 497, 598
TseI GCWGC 1 cut(s) 437
Tsp45I GTSAC 2 cut(s) 497, 598
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 1 cut(s) 245
Van91I CCANNNNNTGG 1 cut(s) 308
Vha464I CTTAAG 1 cut(s) 568
VpaK11BI GGWCC 2 cut(s) 107, 241
XapI RAATTY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 1 cut(s) 606
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.