FvH4_1g00940

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0011000
Physical Location & Seq
Forward (+)
459277 .. 461670
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00940.t3

Sequence Viewer

Length: 657 bp
ATGGTGGCGGCGAATCTGAAGGCGGAGACTTTGGGTCTGATGGACAAACGGAGTGCTTTGGAGGCCGAGATGAACGCAATCATCGAGCGTCTTACTCAGCCCGGCGGCCCTGGACTTTCCGGCAACCTCCTCAACTTCGAGGGGTTTCCTCGCGAGGACATTGACATCCCAGCTGTACGAGCCGAACGACGTCGTCTTGCTGAGCTGAAAAATGATTACAAAGAGATTACGGAGAAATTGGACCGGAATATACAAGTTTTGCATTCAGCAAAACTTGCTCCAAAATCATCATCCCTCAACCCGTCAGATGGTCAGAGTGGTTCAGTTGTCAGTGTTGGAGAATCAGCTTCTGCAACCAATGTTAATTCAAATGCCATGGATGTGGATGTGAGTGTACCCTTTGCATTGGTTGATGAGATAGCTGATGCATCGCCAGCGGCAGAGGATGGTTTTCAACTTGGAGATCAGATTGTGAAGTTTGGCAATGTGGAAAATGGCGACAATTTGTTGCAGAAACTTGCTTCTGAGACTCAAACGAATCAGGGTTGTGCTATACCTGTTATGGTCCTAAGGCAAGGTGCCCAAGTGAACTTAATGGTGACACCTAGAACATGGCAAGGCAGGGGTCTACTTGGGTGTCATTTCCAGATTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

219

Amino Acids

23.37

Weight (kDa)

4.85

Isoelectric Point (pI)

35.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nas2_N PF18265 13 - 89 2.1e-21 Nas2 N_terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57950 AT5G57950
fragaria_vesca FvH4_1g00940 FvH4_1g00940 FvH4_1g00940
malus_domestica MD02G1007700.v1.1 MD09G1175000.v1.1
prunus_persica Prupe.7G262800_v2.0.a1
pyrus_communis pycom02g00660 pycom15g13650
rosa_chinensis RchiOBHm_Chr2g0085671
rosa_laevigata RLG00000015710
rosa_multiflora Rmu_co8318769.1_g000001 Rmu_sc0007631.1_g000007
rosa_roxburghii Rroxscaffold_2G00155140
rosa_rugosa Rorug01G0462100
rosa_samantha Rh2AG011300 Rh2BG011800 Rh2CG012600
rosa_wichuraiana Rw2G001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 578
AccI GTMKAC 1 cut(s) 628
AccII CGCG 1 cut(s) 153
AciI CCGC 4 cut(s) 8, 23, 105, 437
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 2 cut(s) 177, 396
AfiI CCNNNNNNNGG 1 cut(s) 308
AgsI TTSAA 2 cut(s) 369, 455
AhdI GACNNNNNGTC 1 cut(s) 33
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 4 cut(s) 173, 205, 347, 422
AluI AGCT 4 cut(s) 173, 205, 347, 422
Alw26I GTCTC 2 cut(s) 20, 521
AlwNI CAGNNNCTG 1 cut(s) 350
AoxI GGCC 2 cut(s) 63, 106
AspS9I GGNCC 3 cut(s) 107, 241, 565
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 610
AvaII GGWCC 2 cut(s) 241, 565
AxyI CCTNAGG 1 cut(s) 569
BaeGI GKGCMC 1 cut(s) 583
BanI GGYRCC 1 cut(s) 578
BccI CCATC 3 cut(s) 34, 302, 440
BciT130I CCWGG 1 cut(s) 111
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 20, 521
BfaI CTAG 1 cut(s) 606
BisI GCNGC 3 cut(s) 9, 106, 438
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 3 cut(s) 10, 107, 439
Bme1390I CCNGG 2 cut(s) 102, 111
Bme18I GGWCC 2 cut(s) 241, 565
BmeRI GACNNNNNGTC 1 cut(s) 33
BmgT120I GGNCC 3 cut(s) 107, 241, 565
BmiI GGNNCC 1 cut(s) 580
BmrFI CCNGG 2 cut(s) 102, 111
BmsI GCATC 2 cut(s) 415, 437
Bpu1102I GCTNAGC 1 cut(s) 201
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 2 cut(s) 109, 375
BsaWI WCCGGW 1 cut(s) 243
Bsc4I CCNNNNNNNGG 1 cut(s) 308
Bse21I CCTNAGG 1 cut(s) 569
Bse3DI GCAATG 1 cut(s) 490
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 2 cut(s) 109, 375
BseGI GGATG 5 cut(s) 165, 290, 385, 391, 451
BseLI CCNNNNNNNGG 1 cut(s) 308
BseMI GCAATG 1 cut(s) 490
BseMII CTCAG 3 cut(s) 110, 192, 516
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 583
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 2 cut(s) 65, 108
BshNI GGYRCC 1 cut(s) 578
BsiSI CCGG 3 cut(s) 102, 120, 244
BslI CCNNNNNNNGG 1 cut(s) 308
BsmAI GTCTC 2 cut(s) 20, 521
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 2 cut(s) 65, 108
Bsp1286I GDGCHC 1 cut(s) 583
Bsp143I GATC 1 cut(s) 463
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp19I CCATGG 1 cut(s) 375
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 4 cut(s) 8, 23, 105, 437
BspANI GGCC 2 cut(s) 65, 108
BspCNI CTCAG 3 cut(s) 109, 193, 517
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 580
BspT107I GGYRCC 1 cut(s) 578
BsrDI GCAATG 1 cut(s) 490
BssECI CCNNGG 2 cut(s) 109, 375
BssMI GATC 1 cut(s) 463
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 111
BstACI GRCGYC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 275
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 4 cut(s) 96, 201, 525, 569
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 5 cut(s) 165, 290, 385, 391, 451
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 1 cut(s) 466
BstMAI GTCTC 2 cut(s) 20, 521
BstMBI GATC 1 cut(s) 463
BstMWI GCNNNNNNNGC 4 cut(s) 62, 179, 275, 434
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 100, 109
BstSLI GKGCMC 1 cut(s) 583
BstUI CGCG 1 cut(s) 153
BstXI CCANNNNNNTGG 1 cut(s) 382
Bsu36I CCTNAGG 1 cut(s) 569
BsuRI GGCC 2 cut(s) 65, 108
BtgI CCRYGG 1 cut(s) 375
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 5 cut(s) 165, 290, 385, 391, 451
BtsIMutI CAGTG 1 cut(s) 337
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 350
Cfr13I GGNCC 3 cut(s) 107, 241, 565
CseI GACGC 1 cut(s) 77
Csp6I GTAC 2 cut(s) 176, 395
CviAII CATG 2 cut(s) 376, 612
CviJI RGCY 8 cut(s) 65, 100, 108, 173, 182, 205, 347, 422
CviKI_1 RGCY 8 cut(s) 65, 100, 108, 173, 182, 205, 347, 422
CviQI GTAC 2 cut(s) 176, 395
DdeI CTNAG 4 cut(s) 96, 201, 525, 569
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
DriI GACNNNNNGTC 1 cut(s) 33
Eam1105I GACNNNNNGTC 1 cut(s) 33
EciI GGCGGA 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 375
Eco47I GGWCC 2 cut(s) 241, 565
Eco57I CTGAAG 1 cut(s) 38
Eco81I CCTNAGG 1 cut(s) 569
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 375
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 1 cut(s) 375
FaeI CATG 2 cut(s) 379, 615
FaiI YATR 5 cut(s) 251, 377, 554, 563, 613
FatI CATG 2 cut(s) 375, 611
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 3 cut(s) 9, 106, 438
FokI GGATG 5 cut(s) 152, 277, 392, 398, 458
Fsp4HI GCNGC 3 cut(s) 9, 106, 438
FspBI CTAG 1 cut(s) 606
GluI GCNGC 3 cut(s) 9, 106, 438
GsaI CCCAGC 1 cut(s) 173
HaeIII GGCC 2 cut(s) 65, 108
HapII CCGG 3 cut(s) 102, 120, 244
HgaI GACGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 2 cut(s) 379, 615
HinfI GANTC 4 cut(s) 13, 341, 529, 538
HpaII CCGG 3 cut(s) 102, 120, 244
HphI GGTGA 1 cut(s) 610
Hpy166II GTNNAC 3 cut(s) 395, 589, 629
Hpy188I TCNGA 6 cut(s) 18, 39, 307, 315, 468, 526
Hpy188III TCNNGA 2 cut(s) 152, 646
Hpy8I GTNNAC 3 cut(s) 395, 589, 629
Hpy99I CGWCG 2 cut(s) 192, 195
HpyAV CCTTC 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 5 cut(s) 262, 353, 404, 428, 511
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 179, 275, 434
HpyF3I CTNAG 4 cut(s) 96, 201, 525, 569
HpySE526I ACGT 1 cut(s) 190
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 2 cut(s) 379, 615
Kzo9I GATC 1 cut(s) 463
LmnI GCTCC 1 cut(s) 283
LweI GCATC 2 cut(s) 415, 437
MaeI CTAG 1 cut(s) 606
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 1 cut(s) 598
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MhlI GDGCHC 1 cut(s) 583
MluCI AATT 3 cut(s) 236, 364, 502
MlyI GAGTC 1 cut(s) 523
MmeI TCCRAC 1 cut(s) 316
MnlI CCTC 8 cut(s) 55, 133, 137, 140, 148, 159, 305, 436
Mph1103I ATGCAT 1 cut(s) 430
MseI TTAA 2 cut(s) 363, 593
MslI CAYNNNNRTG 1 cut(s) 380
MspA1I CMGCKG 2 cut(s) 173, 437
MspI CCGG 3 cut(s) 102, 120, 244
MspR9I CCNGG 2 cut(s) 102, 111
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 4 cut(s) 62, 179, 275, 434
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 375
NdeII GATC 1 cut(s) 463
NlaIII CATG 2 cut(s) 379, 615
NlaIV GGNNCC 1 cut(s) 580
NmeAIII GCCGAG 1 cut(s) 91
NmuCI GTSAC 1 cut(s) 598
NruI TCGCGA 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 430
PcsI WCGNNNNNNNCGW 2 cut(s) 81, 184
PctI GAATGC 1 cut(s) 262
PfeI GAWTC 3 cut(s) 13, 341, 538
PflFI GACNNNGTC 1 cut(s) 192
PkrI GCNGC 3 cut(s) 10, 107, 439
PleI GAGTC 1 cut(s) 523
PpsI GAGTC 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 580
PspPI GGNCC 3 cut(s) 107, 241, 565
PstNI CAGNNNCTG 1 cut(s) 350
PsyI GACNNNGTC 1 cut(s) 192
PvuII CAGCTG 1 cut(s) 173
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 2 cut(s) 177, 396
RsaNI GTAC 2 cut(s) 176, 395
RseI CAYNNNNRTG 1 cut(s) 380
SaqAI TTAA 2 cut(s) 363, 593
SatI GCNGC 3 cut(s) 9, 106, 438
Sau3AI GATC 1 cut(s) 463
Sau96I GGNCC 3 cut(s) 107, 241, 565
SchI GAGTC 1 cut(s) 523
ScrFI CCNGG 2 cut(s) 102, 111
SduI GDGCHC 1 cut(s) 583
SetI ASST 9 cut(s) 129, 175, 193, 207, 349, 424, 559, 580, 607
SfaNI GCATC 2 cut(s) 415, 437
SinI GGWCC 2 cut(s) 241, 565
SmiMI CAYNNNNRTG 1 cut(s) 380
Sse9I AATT 3 cut(s) 236, 364, 502
SsiI CCGC 4 cut(s) 8, 23, 105, 437
SspMI CTAG 1 cut(s) 606
StyD4I CCNGG 2 cut(s) 100, 109
StyI CCWWGG 1 cut(s) 375
TaiI ACGT 1 cut(s) 193
TaqI TCGA 2 cut(s) 84, 138
TasI AATT 3 cut(s) 236, 364, 502
TauI GCSGC 3 cut(s) 11, 108, 440
TfiI GAWTC 3 cut(s) 13, 341, 538
Tru1I TTAA 2 cut(s) 363, 593
Tru9I TTAA 2 cut(s) 363, 593
TscAI CASTG 1 cut(s) 337
TseFI GTSAC 1 cut(s) 598
Tsp45I GTSAC 1 cut(s) 598
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 2 cut(s) 64, 245
TspRI CASTG 1 cut(s) 337
Tth111I GACNNNGTC 1 cut(s) 192
VpaK11BI GGWCC 2 cut(s) 241, 565
XmiI GTMKAC 1 cut(s) 628
XspI CTAG 1 cut(s) 606
ZraI GACGTC 1 cut(s) 191
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.