pycom02g00660

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0011000
Physical Location & Seq
Forward (+)
360344 .. 362341
1998 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00660.2

Sequence Viewer

Length: 810 bp
ATGTTTCTCGGTGTTTGCAGTTTGAAATTCGATGGTGGTTACCCTTGGCTGATCGCCGCCGGTTTTATTCTCTCTGGTTCTCTCAGTCTCAACTCAAGTACAGAAAAGAAAAGAAAAAAAGGGGAAGAGAAGGAAAAAATGGTGGGAACGAATCTGAAGGGGGAGACGATGGACCTGATGGAGAAGAGGACTGCCCTGGAGGCGGAGATGAACGCTATCATCGAGCTTCTTACTCAGCCCGGCGGCCCTGGAATCTCCGGCAACCTCCTTGACTCCGAGGGTTTTCCTCGCGAAGACATTGACATCCCAGCTGTGCGAGCGGAAAGGCGTCGTCTTGCTGAATTGCGAAATGATCACAAAGAGGTTACCGAGAAATTGAACCGAAATATAGAAGTTCTGCATTCGGCAAGGCTTATTCCGAAAAAATCATCACTTAAAGATTCAGATGGCCAAAGCGCATCAGTTGTCAACGTGGTTGCATCTGCATCCTCAACCAATGTTCATACTGGTTCTATCAATGCCATGGATGTGGATGTGATTGTGAGCGTACCCTTTGTGTTGGTGGATGAGATAGCTGATGAATCACCAGCAGCAGAGGATGGTTTGCAGCTTGGAGATCAGATTGTTAAATTTGGTAGTGTGGAAAATGGCGATAATCTGTTGCAGAAACTTGCTTCTGAGGCTCAAGGTAATCAGGGCCGTGGGATACCTGTAATACTTGTGAGGCAAGGTGCTCAAGTCAACTTAACCATGACACCTAGAACATGGCAAGGCAGGGGTATACTCGGCTGCCATTTCCGGATCCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

270

Amino Acids

29.09

Weight (kDa)

5.53

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57950 AT5G57950
fragaria_vesca FvH4_1g00940 FvH4_1g00940 FvH4_1g00940
malus_domestica MD02G1007700.v1.1 MD09G1175000.v1.1
prunus_persica Prupe.7G262800_v2.0.a1
pyrus_communis pycom02g00660 pycom15g13650
rosa_chinensis RchiOBHm_Chr2g0085671
rosa_laevigata RLG00000015710
rosa_multiflora Rmu_co8318769.1_g000001 Rmu_sc0007631.1_g000007
rosa_roxburghii Rroxscaffold_2G00155140
rosa_rugosa Rorug01G0462100
rosa_samantha Rh2AG011300 Rh2BG011800 Rh2CG012600
rosa_wichuraiana Rw2G001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 320
AccI GTMKAC 1 cut(s) 781
AccII CGCG 1 cut(s) 291
AccIII TCCGGA 1 cut(s) 798
AciI CCGC 4 cut(s) 57, 203, 243, 320
AclWI GGATC 2 cut(s) 796, 809
AcoI YGGCCR 1 cut(s) 448
AcsI RAATTY 2 cut(s) 26, 629
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 1 cut(s) 328
AfaI GTAC 2 cut(s) 100, 549
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 2 cut(s) 25, 379
AjnI CCWGG 2 cut(s) 195, 247
AluBI AGCT 4 cut(s) 226, 311, 575, 610
AluI AGCT 4 cut(s) 226, 311, 575, 610
Alw21I GWGCWC 1 cut(s) 736
Alw26I GTCTC 2 cut(s) 92, 158
AlwI GGATC 2 cut(s) 796, 809
Aor13HI TCCGGA 1 cut(s) 798
AoxI GGCC 3 cut(s) 244, 448, 697
ApeKI GCWGC 3 cut(s) 590, 607, 789
ApoI RAATTY 2 cut(s) 26, 629
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 3 cut(s) 172, 245, 697
AsuC2I CCSGG 1 cut(s) 240
AsuHPI GGTGA 1 cut(s) 576
AvaII GGWCC 1 cut(s) 172
BalI TGGCCA 1 cut(s) 450
BamHI GGATCC 1 cut(s) 801
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 1 cut(s) 736
BbvI GCAGC 3 cut(s) 602, 619, 776
BccI CCATC 5 cut(s) 26, 163, 172, 440, 593
BceAI ACGGC 1 cut(s) 684
BciT130I CCWGG 2 cut(s) 197, 249
BciVI GTATCC 1 cut(s) 699
BclI TGATCA 1 cut(s) 352
BcnI CCSGG 1 cut(s) 240
BcoDI GTCTC 2 cut(s) 92, 158
BfaI CTAG 1 cut(s) 759
BfuI GTATCC 1 cut(s) 699
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 5 cut(s) 57, 244, 591, 608, 790
BlsI GCNGC 5 cut(s) 58, 245, 592, 609, 791
Bme1390I CCNGG 3 cut(s) 197, 240, 249
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 3 cut(s) 172, 245, 697
BmiI GGNNCC 1 cut(s) 803
BmrFI CCNGG 3 cut(s) 197, 240, 249
BmsI GCATC 3 cut(s) 467, 488, 494
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 1 cut(s) 218
BpuEI CTTGAG 3 cut(s) 79, 669, 720
BpuMI CCSGG 1 cut(s) 240
BsaHI GRCGYC 1 cut(s) 328
BsaJI CCNNGG 6 cut(s) 44, 195, 247, 276, 522, 700
BsaWI WCCGGW 1 cut(s) 798
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 1 cut(s) 511
BseAI TCCGGA 1 cut(s) 798
BseBI CCWGG 2 cut(s) 197, 249
BseDI CCNNGG 6 cut(s) 44, 195, 247, 276, 522, 700
BseGI GGATG 6 cut(s) 303, 485, 532, 538, 571, 604
BseLI CCNNNNNNNGG 1 cut(s) 202
BseMII CTCAG 3 cut(s) 97, 248, 669
BseNI ACTGG 1 cut(s) 511
BseXI GCAGC 3 cut(s) 602, 619, 776
BseYI CCCAGC 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 3 cut(s) 246, 450, 699
BsiHKAI GWGCWC 1 cut(s) 736
BsiSI CCGG 4 cut(s) 60, 240, 258, 799
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 2 cut(s) 92, 158
BsmBI CGTCTC 1 cut(s) 158
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 3 cut(s) 246, 450, 699
Bsp1286I GDGCHC 1 cut(s) 736
Bsp13I TCCGGA 1 cut(s) 798
Bsp143I GATC 4 cut(s) 51, 352, 616, 801
Bsp19I CCATGG 1 cut(s) 522
Bsp68I TCGCGA 1 cut(s) 291
BspACI CCGC 4 cut(s) 57, 203, 243, 320
BspANI GGCC 3 cut(s) 246, 450, 699
BspCNI CTCAG 3 cut(s) 96, 247, 670
BspEI TCCGGA 1 cut(s) 798
BspFNI CGCG 1 cut(s) 291
BspLI GGNNCC 1 cut(s) 803
BspPI GGATC 2 cut(s) 796, 809
BsrBI CCGCTC 1 cut(s) 320
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 1 cut(s) 511
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 6 cut(s) 44, 195, 247, 276, 522, 700
BssMI GATC 4 cut(s) 51, 352, 616, 801
BssNAI GTATAC 1 cut(s) 782
BssNI GRCGYC 1 cut(s) 328
BssT1I CCWWGG 2 cut(s) 44, 522
Bst1107I GTATAC 1 cut(s) 782
Bst2UI CCWGG 2 cut(s) 197, 249
Bst6I CTCTTC 2 cut(s) 120, 179
BstACI GRCGYC 1 cut(s) 328
BstC8I GCNNGC 1 cut(s) 318
BstDEI CTNAG 3 cut(s) 83, 234, 678
BstDSI CCRYGG 2 cut(s) 522, 700
BstEII GGTNACC 2 cut(s) 38, 364
BstF5I GGATG 6 cut(s) 303, 485, 532, 538, 571, 604
BstFNI CGCG 1 cut(s) 291
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 4 cut(s) 54, 355, 619, 804
BstMAI GTCTC 2 cut(s) 92, 158
BstMBI GATC 4 cut(s) 51, 352, 616, 801
BstMWI GCNNNNNNNGC 3 cut(s) 200, 317, 680
BstNI CCWGG 2 cut(s) 197, 249
BstPI GGTNACC 2 cut(s) 38, 364
BstSCI CCNGG 3 cut(s) 195, 238, 247
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 3 cut(s) 602, 619, 776
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 801
BstXI CCANNNNNNTGG 1 cut(s) 529
BstYI RGATCY 1 cut(s) 801
BstZ17I GTATAC 1 cut(s) 782
BsuI GTATCC 1 cut(s) 699
BsuRI GGCC 3 cut(s) 246, 450, 699
BtgI CCRYGG 2 cut(s) 522, 700
BtsCI GGATG 6 cut(s) 303, 485, 532, 538, 571, 604
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 318
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 3 cut(s) 172, 245, 697
CseI GACGC 1 cut(s) 317
Csp6I GTAC 2 cut(s) 99, 548
CviAII CATG 3 cut(s) 523, 751, 765
CviQI GTAC 2 cut(s) 99, 548
DdeI CTNAG 3 cut(s) 83, 234, 678
DpnI GATC 4 cut(s) 53, 354, 618, 803
DpnII GATC 4 cut(s) 51, 352, 616, 801
EaeI YGGCCR 1 cut(s) 448
Eam1104I CTCTTC 2 cut(s) 120, 179
EarI CTCTTC 2 cut(s) 120, 179
EciI GGCGGA 1 cut(s) 218
Eco130I CCWWGG 2 cut(s) 44, 522
Eco47I GGWCC 1 cut(s) 172
Eco57I CTGAAG 1 cut(s) 176
Eco91I GGTNACC 2 cut(s) 38, 364
EcoO65I GGTNACC 2 cut(s) 38, 364
EcoRII CCWGG 2 cut(s) 195, 247
EcoT14I CCWWGG 2 cut(s) 44, 522
ErhI CCWWGG 2 cut(s) 44, 522
Esp3I CGTCTC 1 cut(s) 158
FaeI CATG 3 cut(s) 526, 754, 768
FaiI YATR 6 cut(s) 389, 504, 524, 752, 766, 782
FatI CATG 3 cut(s) 522, 750, 764
FbaI TGATCA 1 cut(s) 352
FblI GTMKAC 1 cut(s) 781
Fnu4HI GCNGC 5 cut(s) 57, 244, 591, 608, 790
FokI GGATG 6 cut(s) 290, 472, 539, 545, 578, 611
Fsp4HI GCNGC 5 cut(s) 57, 244, 591, 608, 790
FspBI CTAG 1 cut(s) 759
GlaI GCGC 1 cut(s) 457
GluI GCNGC 5 cut(s) 57, 244, 591, 608, 790
GsaI CCCAGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 3 cut(s) 246, 450, 699
HapII CCGG 4 cut(s) 60, 240, 258, 799
HgaI GACGC 1 cut(s) 317
HhaI GCGC 1 cut(s) 458
Hin1I GRCGYC 1 cut(s) 328
Hin1II CATG 3 cut(s) 526, 754, 768
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 2 cut(s) 469, 742
HindII GTYRAC 2 cut(s) 469, 742
HinfI GANTC 5 cut(s) 151, 252, 272, 440, 581
HpaII CCGG 4 cut(s) 60, 240, 258, 799
HphI GGTGA 1 cut(s) 576
Hpy166II GTNNAC 3 cut(s) 469, 742, 782
Hpy188I TCNGA 6 cut(s) 156, 277, 420, 445, 621, 679
Hpy188III TCNNGA 2 cut(s) 290, 799
Hpy8I GTNNAC 3 cut(s) 469, 742, 782
Hpy99I CGWCG 1 cut(s) 333
HpyAV CCTTC 2 cut(s) 124, 151
HpyCH4IV ACGT 1 cut(s) 471
HpyCH4V TGCA 6 cut(s) 18, 400, 479, 485, 607, 664
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 317, 680
HpyF3I CTNAG 3 cut(s) 83, 234, 678
HpySE526I ACGT 1 cut(s) 471
Hsp92I GRCGYC 1 cut(s) 328
Hsp92II CATG 3 cut(s) 526, 754, 768
HspAI GCGC 1 cut(s) 456
Kpn2I TCCGGA 1 cut(s) 798
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 4 cut(s) 51, 352, 616, 801
Lsp1109I GCAGC 3 cut(s) 602, 619, 776
LweI GCATC 3 cut(s) 467, 488, 494
MaeI CTAG 1 cut(s) 759
MaeII ACGT 1 cut(s) 471
MaeIII GTNAC 2 cut(s) 38, 364
MalI GATC 4 cut(s) 53, 354, 618, 803
MbiI CCGCTC 1 cut(s) 320
MboI GATC 4 cut(s) 51, 352, 616, 801
MboII GAAGA 3 cut(s) 137, 196, 305
MflI RGATCY 1 cut(s) 801
MhlI GDGCHC 1 cut(s) 736
MlsI TGGCCA 1 cut(s) 450
MluCI AATT 4 cut(s) 26, 341, 374, 629
MluNI TGGCCA 1 cut(s) 450
MlyI GAGTC 1 cut(s) 266
Mox20I TGGCCA 1 cut(s) 450
MroI TCCGGA 1 cut(s) 798
MscI TGGCCA 1 cut(s) 450
MseI TTAA 3 cut(s) 435, 627, 746
MslI CAYNNNNRTG 1 cut(s) 527
Msp20I TGGCCA 1 cut(s) 450
MspA1I CMGCKG 1 cut(s) 311
MspI CCGG 4 cut(s) 60, 240, 258, 799
MspR9I CCNGG 3 cut(s) 197, 240, 249
Mva1269I GAATGC 1 cut(s) 400
MvaI CCWGG 2 cut(s) 197, 249
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 200, 317, 680
NciI CCSGG 1 cut(s) 240
NcoI CCATGG 1 cut(s) 522
NdeII GATC 4 cut(s) 51, 352, 616, 801
NlaIII CATG 3 cut(s) 526, 754, 768
NlaIV GGNNCC 1 cut(s) 803
NmeAIII GCCGAG 1 cut(s) 765
NruI TCGCGA 1 cut(s) 291
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 400
PfeI GAWTC 4 cut(s) 151, 252, 440, 581
PkrI GCNGC 5 cut(s) 58, 245, 592, 609, 791
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 195, 247
PspEI GGTNACC 2 cut(s) 38, 364
PspFI CCCAGC 1 cut(s) 307
PspGI CCWGG 2 cut(s) 195, 247
PspN4I GGNNCC 1 cut(s) 803
PspPI GGNCC 3 cut(s) 172, 245, 697
PsuI RGATCY 1 cut(s) 801
PvuII CAGCTG 1 cut(s) 311
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 2 cut(s) 100, 549
RsaNI GTAC 2 cut(s) 99, 548
RseI CAYNNNNRTG 1 cut(s) 527
SaqAI TTAA 3 cut(s) 435, 627, 746
SatI GCNGC 5 cut(s) 57, 244, 591, 608, 790
Sau3AI GATC 4 cut(s) 51, 352, 616, 801
Sau96I GGNCC 3 cut(s) 172, 245, 697
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 3 cut(s) 197, 240, 249
SduI GDGCHC 1 cut(s) 736
SfaNI GCATC 3 cut(s) 467, 488, 494
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 527
SmlI CTYRAG 3 cut(s) 94, 684, 735
SmoI CTYRAG 3 cut(s) 94, 684, 735
Sse9I AATT 4 cut(s) 26, 341, 374, 629
SsiI CCGC 4 cut(s) 57, 203, 243, 320
SspMI CTAG 1 cut(s) 759
StyD4I CCNGG 3 cut(s) 195, 238, 247
StyI CCWWGG 2 cut(s) 44, 522
TaiI ACGT 1 cut(s) 474
TaqI TCGA 2 cut(s) 30, 222
TasI AATT 4 cut(s) 26, 341, 374, 629
TatI WGTACW 1 cut(s) 98
TauI GCSGC 2 cut(s) 59, 246
TfiI GAWTC 4 cut(s) 151, 252, 440, 581
Tru1I TTAA 3 cut(s) 435, 627, 746
Tru9I TTAA 3 cut(s) 435, 627, 746
TseI GCWGC 3 cut(s) 590, 607, 789
TspDTI ATGAA 3 cut(s) 224, 491, 594
VpaK11BI GGWCC 1 cut(s) 172
XapI RAATTY 2 cut(s) 26, 629
XmiI GTMKAC 1 cut(s) 781
XspI CTAG 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.