MD02G1007700.v1.1

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0011000
Physical Location & Seq
Forward (+)
487844 .. 490037
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1007700.v1.1.491

Sequence Viewer

Length: 672 bp
ATGGTGCGAACGAATCTGAAGGGGGAGACGATGGGCCTGATGGAGAAGAGGACTGCCCTGGAGGCGGAGATCAACGCCATCATCGAGCGTCTTACTCAGCCCGGCGGCCCTGGAATCTCCGGCAACCTCCTTGACTCCGAGGGTTTTCCTCGCGAAGACATTGACATCCCAGCTGTGCGAGCGGAAAGGCGTCGTCTTGCGGAATTGCGAAATGATCACAAAGAGGTTACCGAGAAATTGAACCGAAATATAGAAGTTCTGCATTCGGCAAGGCTTACTCCGAAACAATCATCACTCAAAGATTCAGATGGCCAAAGCGCATCAGTTGTCAACGTGGTTGTATCTGCATCCTCAACCAATGTTCATACTGGTTCTACCAATACCATGGATGTGGATGTGATTGTGAGTGTACCCTTTGCGTTGGTGGATGAGATAGCTGATGAATCACCAGCAGCAGAGGATGGTTTGCAGCTTGGAGATCAGATTGTTAAATTTGGTAGTGTAGAAAATGGCGATAATTTGTTGCAGAAACTTGCTTCCGAGGCTCAAGCTAATCAGGGCCGTGGGATACCTGTAATACTTGTAAGGCAAGGTGCTCAAGTCAACTTAACCATGACACCTAGAACATGGCAAGGCAGGGGTCTACTCGGCTGCCATTTCCGGATCTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

224

Amino Acids

24.14

Weight (kDa)

5.31

Isoelectric Point (pI)

41.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nas2_N PF18265 12 - 89 1.1e-23 Nas2 N_terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57950 AT5G57950
fragaria_vesca FvH4_1g00940 FvH4_1g00940 FvH4_1g00940
malus_domestica MD02G1007700.v1.1 MD09G1175000.v1.1
prunus_persica Prupe.7G262800_v2.0.a1
pyrus_communis pycom02g00660 pycom15g13650
rosa_chinensis RchiOBHm_Chr2g0085671
rosa_laevigata RLG00000015710
rosa_multiflora Rmu_co8318769.1_g000001 Rmu_sc0007631.1_g000007
rosa_roxburghii Rroxscaffold_2G00155140
rosa_rugosa Rorug01G0462100
rosa_samantha Rh2AG011300 Rh2BG011800 Rh2CG012600
rosa_wichuraiana Rw2G001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 182
AccI GTMKAC 1 cut(s) 643
AccII CGCG 1 cut(s) 153
AccIII TCCGGA 1 cut(s) 660
AciI CCGC 4 cut(s) 65, 105, 182, 200
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 64
AgsI TTSAA 1 cut(s) 241
AjnI CCWGG 2 cut(s) 57, 109
AluBI AGCT 4 cut(s) 173, 437, 472, 551
AluI AGCT 4 cut(s) 173, 437, 472, 551
Alw21I GWGCWC 1 cut(s) 598
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 1 cut(s) 671
Aor13HI TCCGGA 1 cut(s) 660
AoxI GGCC 4 cut(s) 34, 106, 310, 559
ApeKI GCWGC 3 cut(s) 452, 469, 651
ApoI RAATTY 1 cut(s) 491
AspLEI GCGC 1 cut(s) 320
AspS9I GGNCC 3 cut(s) 34, 107, 559
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 438
BalI TGGCCA 1 cut(s) 312
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 1 cut(s) 598
BbvI GCAGC 3 cut(s) 464, 481, 638
BccI CCATC 5 cut(s) 25, 34, 86, 302, 455
BceAI ACGGC 1 cut(s) 546
BciT130I CCWGG 2 cut(s) 59, 111
BciVI GTATCC 1 cut(s) 561
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 621
BfuI GTATCC 1 cut(s) 561
BglI GCCNNNNNGGC 1 cut(s) 62
BisI GCNGC 4 cut(s) 106, 453, 470, 652
BlsI GCNGC 4 cut(s) 107, 454, 471, 653
Bme1390I CCNGG 3 cut(s) 59, 102, 111
BmgT120I GGNCC 3 cut(s) 34, 107, 559
BmrFI CCNGG 3 cut(s) 59, 102, 111
BmsI GCATC 2 cut(s) 329, 356
BpiI GAAGAC 1 cut(s) 162
BpmI CTGGAG 1 cut(s) 80
BpuEI CTTGAG 2 cut(s) 531, 582
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 6 cut(s) 57, 109, 138, 384, 540, 562
BsaWI WCCGGW 1 cut(s) 660
Bsc4I CCNNNNNNNGG 1 cut(s) 64
Bse1I ACTGG 1 cut(s) 373
BseAI TCCGGA 1 cut(s) 660
BseBI CCWGG 2 cut(s) 59, 111
BseDI CCNNGG 6 cut(s) 57, 109, 138, 384, 540, 562
BseGI GGATG 6 cut(s) 165, 347, 394, 400, 433, 466
BseLI CCNNNNNNNGG 1 cut(s) 64
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 373
BseXI GCAGC 3 cut(s) 464, 481, 638
BseYI CCCAGC 1 cut(s) 169
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 4 cut(s) 36, 108, 312, 561
BsiHKAI GWGCWC 1 cut(s) 598
BsiSI CCGG 3 cut(s) 102, 120, 661
BslI CCNNNNNNNGG 1 cut(s) 64
BsmAI GTCTC 1 cut(s) 20
BsmBI CGTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 4 cut(s) 36, 108, 312, 561
Bsp1286I GDGCHC 1 cut(s) 598
Bsp13I TCCGGA 1 cut(s) 660
Bsp143I GATC 4 cut(s) 69, 214, 478, 663
Bsp19I CCATGG 1 cut(s) 384
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 4 cut(s) 65, 105, 182, 200
BspANI GGCC 4 cut(s) 36, 108, 312, 561
BspCNI CTCAG 1 cut(s) 109
BspEI TCCGGA 1 cut(s) 660
BspFNI CGCG 1 cut(s) 153
BspPI GGATC 1 cut(s) 671
BsrBI CCGCTC 1 cut(s) 182
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 6 cut(s) 57, 109, 138, 384, 540, 562
BssMI GATC 4 cut(s) 69, 214, 478, 663
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 2 cut(s) 59, 111
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 180
BstDEI CTNAG 1 cut(s) 96
BstDSI CCRYGG 2 cut(s) 384, 562
BstEII GGTNACC 1 cut(s) 226
BstF5I GGATG 6 cut(s) 165, 347, 394, 400, 433, 466
BstFNI CGCG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 320
BstKTI GATC 4 cut(s) 72, 217, 481, 666
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 4 cut(s) 69, 214, 478, 663
BstMWI GCNNNNNNNGC 3 cut(s) 62, 179, 542
BstNI CCWGG 2 cut(s) 59, 111
BstPI GGTNACC 1 cut(s) 226
BstSCI CCNGG 3 cut(s) 57, 100, 109
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 3 cut(s) 464, 481, 638
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 663
BstXI CCANNNNNNTGG 2 cut(s) 385, 391
BstYI RGATCY 1 cut(s) 663
BsuI GTATCC 1 cut(s) 561
BsuRI GGCC 4 cut(s) 36, 108, 312, 561
BtgI CCRYGG 2 cut(s) 384, 562
BtsCI GGATG 6 cut(s) 165, 347, 394, 400, 433, 466
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 180
CfoI GCGC 1 cut(s) 320
Cfr13I GGNCC 3 cut(s) 34, 107, 559
CseI GACGC 2 cut(s) 77, 179
Csp6I GTAC 1 cut(s) 410
CviAII CATG 3 cut(s) 385, 613, 627
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 1 cut(s) 96
DpnI GATC 4 cut(s) 71, 216, 480, 665
DpnII GATC 4 cut(s) 69, 214, 478, 663
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 384
Eco57I CTGAAG 1 cut(s) 38
Eco91I GGTNACC 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 226
EcoRII CCWGG 2 cut(s) 57, 109
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 3 cut(s) 388, 616, 630
FaiI YATR 5 cut(s) 251, 366, 386, 614, 628
FatI CATG 3 cut(s) 384, 612, 626
FbaI TGATCA 1 cut(s) 214
FblI GTMKAC 1 cut(s) 643
Fnu4HI GCNGC 4 cut(s) 106, 453, 470, 652
FokI GGATG 6 cut(s) 152, 334, 401, 407, 440, 473
Fsp4HI GCNGC 4 cut(s) 106, 453, 470, 652
FspBI CTAG 1 cut(s) 621
GlaI GCGC 1 cut(s) 319
GluI GCNGC 4 cut(s) 106, 453, 470, 652
GsaI CCCAGC 1 cut(s) 173
GsuI CTGGAG 1 cut(s) 80
HaeIII GGCC 4 cut(s) 36, 108, 312, 561
HapII CCGG 3 cut(s) 102, 120, 661
HgaI GACGC 2 cut(s) 77, 179
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 3 cut(s) 388, 616, 630
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HincII GTYRAC 2 cut(s) 331, 604
HindII GTYRAC 2 cut(s) 331, 604
HinfI GANTC 5 cut(s) 13, 114, 134, 302, 443
HpaII CCGG 3 cut(s) 102, 120, 661
HphI GGTGA 1 cut(s) 438
Hpy166II GTNNAC 4 cut(s) 331, 410, 604, 644
Hpy188I TCNGA 6 cut(s) 18, 139, 282, 307, 483, 541
Hpy188III TCNNGA 2 cut(s) 152, 661
Hpy8I GTNNAC 4 cut(s) 331, 410, 604, 644
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 1 cut(s) 13
HpyCH4IV ACGT 1 cut(s) 333
HpyCH4V TGCA 4 cut(s) 262, 347, 469, 526
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 179, 542
HpyF3I CTNAG 1 cut(s) 96
HpySE526I ACGT 1 cut(s) 333
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 3 cut(s) 388, 616, 630
HspAI GCGC 1 cut(s) 318
Kpn2I TCCGGA 1 cut(s) 660
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 4 cut(s) 69, 214, 478, 663
Lsp1109I GCAGC 3 cut(s) 464, 481, 638
LweI GCATC 2 cut(s) 329, 356
MaeI CTAG 1 cut(s) 621
MaeII ACGT 1 cut(s) 333
MaeIII GTNAC 1 cut(s) 226
MalI GATC 4 cut(s) 71, 216, 480, 665
MbiI CCGCTC 1 cut(s) 182
MboI GATC 4 cut(s) 69, 214, 478, 663
MboII GAAGA 2 cut(s) 58, 167
MflI RGATCY 1 cut(s) 663
MhlI GDGCHC 1 cut(s) 598
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 4 cut(s) 203, 236, 491, 517
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 128
MnlI CCTC 9 cut(s) 42, 55, 133, 137, 159, 217, 361, 451, 535
Mox20I TGGCCA 1 cut(s) 312
MroI TCCGGA 1 cut(s) 660
MscI TGGCCA 1 cut(s) 312
MseI TTAA 2 cut(s) 489, 608
MslI CAYNNNNRTG 1 cut(s) 389
Msp20I TGGCCA 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 3 cut(s) 102, 120, 661
MspR9I CCNGG 3 cut(s) 59, 102, 111
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 2 cut(s) 59, 111
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 3 cut(s) 62, 179, 542
NciI CCSGG 1 cut(s) 102
NcoI CCATGG 1 cut(s) 384
NdeII GATC 4 cut(s) 69, 214, 478, 663
NlaIII CATG 3 cut(s) 388, 616, 630
NmeAIII GCCGAG 1 cut(s) 627
NruI TCGCGA 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 81
PctI GAATGC 1 cut(s) 262
PfeI GAWTC 4 cut(s) 13, 114, 302, 443
PkrI GCNGC 4 cut(s) 107, 454, 471, 653
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
Psp6I CCWGG 2 cut(s) 57, 109
PspEI GGTNACC 1 cut(s) 226
PspFI CCCAGC 1 cut(s) 169
PspGI CCWGG 2 cut(s) 57, 109
PspPI GGNCC 3 cut(s) 34, 107, 559
PsuI RGATCY 1 cut(s) 663
PvuII CAGCTG 1 cut(s) 173
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 389
SaqAI TTAA 2 cut(s) 489, 608
SatI GCNGC 4 cut(s) 106, 453, 470, 652
Sau3AI GATC 4 cut(s) 69, 214, 478, 663
Sau96I GGNCC 3 cut(s) 34, 107, 559
SchI GAGTC 1 cut(s) 128
ScrFI CCNGG 3 cut(s) 59, 102, 111
SduI GDGCHC 1 cut(s) 598
SfaNI GCATC 2 cut(s) 329, 356
SmiMI CAYNNNNRTG 1 cut(s) 389
SmlI CTYRAG 2 cut(s) 546, 597
SmoI CTYRAG 2 cut(s) 546, 597
Sse9I AATT 4 cut(s) 203, 236, 491, 517
SsiI CCGC 4 cut(s) 65, 105, 182, 200
SspMI CTAG 1 cut(s) 621
StyD4I CCNGG 3 cut(s) 57, 100, 109
StyI CCWWGG 1 cut(s) 384
TaiI ACGT 1 cut(s) 336
TaqI TCGA 1 cut(s) 84
TasI AATT 4 cut(s) 203, 236, 491, 517
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 4 cut(s) 13, 114, 302, 443
Tru1I TTAA 2 cut(s) 489, 608
Tru9I TTAA 2 cut(s) 489, 608
TseI GCWGC 3 cut(s) 452, 469, 651
TspDTI ATGAA 2 cut(s) 353, 456
XapI RAATTY 1 cut(s) 491
XmiI GTMKAC 1 cut(s) 643
XspI CTAG 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.