Rw2G001000

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0011000
Physical Location & Seq
Forward (+)
840993 .. 842842
1850 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001000.1

Sequence Viewer

Length: 648 bp
ATGGTGGCGGCGAATCTGAAGGCGGAGACTTTGGGTCTGATGGAGAAGAGGAGTGCTTTGGAGGCGGAGATGAATGCCATCATCGAGCGTCTTACTCGGCCCGGCGGTCCTGGACTTTCCGGCAACCTCCTTAACTTCGAGGGGTTTCCTCGCGAGGACATTGACATCCCAGCTGTGCGAGCTGAACGGCGTCGTCTTGCTGAACTGAAAAATGATTACAAAGAGATTACGGAGAAATTGGACCGAAATATACAAGTTTTGCATTCGGCAAAGCTTGCTCCAAAATCATCATCCCTCAAACCATCAGAGTGGTTCAGTTGTCATGTTGGAGAATCGGCATCTTCAACCAATGTTTCTTCGAATGCGATGGATGTGGATGTGAGCGTACCTTTTGCATTGGTTGATGAGATAGCTGATGCATCACCAGCAGCAGAGGATGGTTTACAGCTTGGAGATCAGATTGTGAAATTTGGCAATGTAGAAAATGGTGACAATTTGTTGCAGAAACTTGCTTCTGAGGCTCAAACGAATCAGGGTTGTGCTGTACCTGTTATGGTCTTAAGGCAAGGTGCCCAAGTCAACTTAATGGTGACACCTAGAACATGGCAAGGCAGGGGTCTGCTGGGGTGTCATTTCCAGATCTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2

Protein Analysis

215

Amino Acids

23.31

Weight (kDa)

5.18

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nas2_N PF18265 13 - 89 1.3e-21 Nas2 N_terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011000)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57950 AT5G57950
fragaria_vesca FvH4_1g00940 FvH4_1g00940 FvH4_1g00940
malus_domestica MD02G1007700.v1.1 MD09G1175000.v1.1
prunus_persica Prupe.7G262800_v2.0.a1
pyrus_communis pycom02g00660 pycom15g13650
rosa_chinensis RchiOBHm_Chr2g0085671
rosa_laevigata RLG00000015710
rosa_multiflora Rmu_co8318769.1_g000001 Rmu_sc0007631.1_g000007
rosa_roxburghii Rroxscaffold_2G00155140
rosa_rugosa Rorug01G0462100
rosa_samantha Rh2AG011300 Rh2BG011800 Rh2CG012600
rosa_wichuraiana Rw2G001000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 569
AccII CGCG 1 cut(s) 153
AciI CCGC 4 cut(s) 8, 23, 65, 105
AcsI RAATTY 1 cut(s) 467
AcuI CTGAAG 1 cut(s) 38
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 2 cut(s) 387, 546
AflII CTTAAG 1 cut(s) 559
AgsI TTSAA 1 cut(s) 345
AhdI GACNNNNNGTC 1 cut(s) 33
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 5 cut(s) 173, 182, 274, 413, 448
AluI AGCT 5 cut(s) 173, 182, 274, 413, 448
Alw26I GTCTC 1 cut(s) 20
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 1 cut(s) 428
ApoI RAATTY 1 cut(s) 467
AspS9I GGNCC 3 cut(s) 99, 107, 241
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 3 cut(s) 414, 500, 601
AsuII TTCGAA 1 cut(s) 359
AvaII GGWCC 2 cut(s) 107, 241
BaeGI GKGCMC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 569
BbvI GCAGC 1 cut(s) 440
BccI CCATC 5 cut(s) 34, 86, 310, 361, 431
BceAI ACGGC 1 cut(s) 203
BciT130I CCWGG 1 cut(s) 111
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 1 cut(s) 597
BfrI CTTAAG 1 cut(s) 559
BglII AGATCT 1 cut(s) 639
BisI GCNGC 2 cut(s) 9, 429
BlsI GCNGC 2 cut(s) 10, 430
Bme1390I CCNGG 2 cut(s) 102, 111
Bme18I GGWCC 2 cut(s) 107, 241
BmeRI GACNNNNNGTC 1 cut(s) 33
BmgT120I GGNCC 3 cut(s) 99, 107, 241
BmiI GGNNCC 1 cut(s) 571
BmrFI CCNGG 2 cut(s) 102, 111
BmsI GCATC 3 cut(s) 347, 406, 428
Bpu14I TTCGAA 1 cut(s) 359
BpuMI CCSGG 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 190
Bse3DI GCAATG 1 cut(s) 481
BseBI CCWGG 1 cut(s) 111
BseGI GGATG 5 cut(s) 165, 290, 376, 382, 442
BseMI GCAATG 1 cut(s) 481
BseMII CTCAG 1 cut(s) 507
BseRI GAGGAG 1 cut(s) 64
BseSI GKGCMC 1 cut(s) 574
BseXI GCAGC 1 cut(s) 440
BseYI CCCAGC 2 cut(s) 169, 622
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 100
BshNI GGYRCC 1 cut(s) 569
BsiSI CCGG 2 cut(s) 102, 120
BsmAI GTCTC 1 cut(s) 20
BsmI GAATGC 3 cut(s) 79, 262, 367
BsnI GGCC 1 cut(s) 100
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 574
Bsp143I GATC 2 cut(s) 454, 639
Bsp68I TCGCGA 1 cut(s) 153
BspACI CCGC 4 cut(s) 8, 23, 65, 105
BspANI GGCC 1 cut(s) 100
BspCNI CTCAG 1 cut(s) 508
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 571
BspT104I TTCGAA 1 cut(s) 359
BspT107I GGYRCC 1 cut(s) 569
BspTI CTTAAG 1 cut(s) 559
BsrDI GCAATG 1 cut(s) 481
BssMI GATC 2 cut(s) 454, 639
BssNI GRCGYC 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 111
Bst6I CTCTTC 1 cut(s) 41
BstACI GRCGYC 1 cut(s) 190
BstAFI CTTAAG 1 cut(s) 559
BstAPI GCANNNNNTGC 1 cut(s) 275
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 2 cut(s) 180, 276
BstDEI CTNAG 1 cut(s) 516
BstF5I GGATG 5 cut(s) 165, 290, 376, 382, 442
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 2 cut(s) 457, 642
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 2 cut(s) 454, 639
BstMWI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 425, 518
BstNI CCWGG 1 cut(s) 111
BstSCI CCNGG 2 cut(s) 100, 109
BstSLI GKGCMC 1 cut(s) 574
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 440
BstX2I RGATCY 1 cut(s) 639
BstXI CCANNNNNNTGG 1 cut(s) 309
BstYI RGATCY 1 cut(s) 639
BsuRI GGCC 1 cut(s) 100
BtgZI GCGATG 1 cut(s) 380
BtsCI GGATG 5 cut(s) 165, 290, 376, 382, 442
BtuMI TCGCGA 1 cut(s) 153
Cac8I GCNNGC 2 cut(s) 180, 276
Cfr13I GGNCC 3 cut(s) 99, 107, 241
CseI GACGC 2 cut(s) 77, 179
Csp6I GTAC 2 cut(s) 386, 545
CviAII CATG 2 cut(s) 323, 603
CviJI RGCY 7 cut(s) 100, 173, 182, 274, 413, 448, 521
CviKI_1 RGCY 7 cut(s) 100, 173, 182, 274, 413, 448, 521
CviQI GTAC 2 cut(s) 386, 545
DdeI CTNAG 1 cut(s) 516
DpnI GATC 2 cut(s) 456, 641
DpnII GATC 2 cut(s) 454, 639
DriI GACNNNNNGTC 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 41
Eam1105I GACNNNNNGTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 41
EciI GGCGGA 2 cut(s) 38, 80
Eco47I GGWCC 2 cut(s) 107, 241
Eco57I CTGAAG 1 cut(s) 38
EcoRII CCWGG 1 cut(s) 109
EcoT22I ATGCAT 1 cut(s) 421
FaeI CATG 2 cut(s) 326, 606
FaiI YATR 4 cut(s) 251, 324, 554, 604
FatI CATG 2 cut(s) 322, 602
Fnu4HI GCNGC 2 cut(s) 9, 429
FokI GGATG 5 cut(s) 152, 277, 383, 389, 449
Fsp4HI GCNGC 2 cut(s) 9, 429
FspBI CTAG 1 cut(s) 597
GluI GCNGC 2 cut(s) 9, 429
GsaI CCCAGC 2 cut(s) 173, 626
HaeIII GGCC 1 cut(s) 100
HapII CCGG 2 cut(s) 102, 120
HgaI GACGC 2 cut(s) 77, 179
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 2 cut(s) 326, 606
HincII GTYRAC 1 cut(s) 580
HindII GTYRAC 1 cut(s) 580
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 3 cut(s) 13, 332, 529
HpaII CCGG 2 cut(s) 102, 120
HphI GGTGA 3 cut(s) 414, 500, 601
Hpy166II GTNNAC 2 cut(s) 443, 580
Hpy188I TCNGA 5 cut(s) 18, 39, 307, 459, 517
Hpy188III TCNNGA 2 cut(s) 152, 637
Hpy8I GTNNAC 2 cut(s) 443, 580
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 1 cut(s) 13
HpyCH4V TGCA 4 cut(s) 262, 395, 419, 502
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 425, 518
HpyF3I CTNAG 1 cut(s) 516
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 2 cut(s) 326, 606
Kzo9I GATC 2 cut(s) 454, 639
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 1 cut(s) 440
LweI GCATC 3 cut(s) 347, 406, 428
MaeI CTAG 1 cut(s) 597
MaeIII GTNAC 2 cut(s) 488, 589
MalI GATC 2 cut(s) 456, 641
MboI GATC 2 cut(s) 454, 639
MboII GAAGA 3 cut(s) 58, 333, 348
MflI RGATCY 1 cut(s) 639
MhlI GDGCHC 1 cut(s) 574
MluCI AATT 3 cut(s) 236, 467, 493
MmeI TCCRAC 1 cut(s) 307
MnlI CCTC 9 cut(s) 42, 55, 133, 137, 148, 159, 305, 427, 511
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 3 cut(s) 132, 560, 584
MslI CAYNNNNRTG 1 cut(s) 307
MspA1I CMGCKG 1 cut(s) 173
MspCI CTTAAG 1 cut(s) 559
MspI CCGG 2 cut(s) 102, 120
MspR9I CCNGG 2 cut(s) 102, 111
Mva1269I GAATGC 3 cut(s) 79, 262, 367
MvaI CCWGG 1 cut(s) 111
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 5 cut(s) 62, 179, 275, 425, 518
NciI CCSGG 1 cut(s) 102
NdeII GATC 2 cut(s) 454, 639
NlaIII CATG 2 cut(s) 326, 606
NlaIV GGNNCC 1 cut(s) 571
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 488, 589
NruI TCGCGA 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 421
NspV TTCGAA 1 cut(s) 359
PctI GAATGC 3 cut(s) 79, 262, 367
PfeI GAWTC 3 cut(s) 13, 332, 529
PfoI TCCNGGA 1 cut(s) 109
PkrI GCNGC 2 cut(s) 10, 430
Psp6I CCWGG 1 cut(s) 109
PspFI CCCAGC 2 cut(s) 169, 622
PspGI CCWGG 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 571
PspPI GGNCC 3 cut(s) 99, 107, 241
PsuI RGATCY 1 cut(s) 639
PvuII CAGCTG 1 cut(s) 173
RruI TCGCGA 1 cut(s) 153
RsaI GTAC 2 cut(s) 387, 546
RsaNI GTAC 2 cut(s) 386, 545
RseI CAYNNNNRTG 1 cut(s) 307
SaqAI TTAA 3 cut(s) 132, 560, 584
SatI GCNGC 2 cut(s) 9, 429
Sau3AI GATC 2 cut(s) 454, 639
Sau96I GGNCC 3 cut(s) 99, 107, 241
ScrFI CCNGG 2 cut(s) 102, 111
SduI GDGCHC 1 cut(s) 574
SfaNI GCATC 3 cut(s) 347, 406, 428
SfuI TTCGAA 1 cut(s) 359
SinI GGWCC 2 cut(s) 107, 241
SmiMI CAYNNNNRTG 1 cut(s) 307
SmlI CTYRAG 1 cut(s) 559
SmoI CTYRAG 1 cut(s) 559
Sse9I AATT 3 cut(s) 236, 467, 493
SsiI CCGC 4 cut(s) 8, 23, 65, 105
SspMI CTAG 1 cut(s) 597
StyD4I CCNGG 2 cut(s) 100, 109
TaqI TCGA 3 cut(s) 84, 138, 359
TaqII GACCGA 1 cut(s) 258
TasI AATT 3 cut(s) 236, 467, 493
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 3 cut(s) 13, 332, 529
Tru1I TTAA 3 cut(s) 132, 560, 584
Tru9I TTAA 3 cut(s) 132, 560, 584
TseFI GTSAC 2 cut(s) 488, 589
TseI GCWGC 1 cut(s) 428
Tsp45I GTSAC 2 cut(s) 488, 589
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 1 cut(s) 245
Vha464I CTTAAG 1 cut(s) 559
VpaK11BI GGWCC 2 cut(s) 107, 241
XapI RAATTY 1 cut(s) 467
XspI CTAG 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.