Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010237

Physical Location & Seq

Genomic Coordinates Reverse (-)

23632228 .. 23635037

Gene Structure 2810 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G58460.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2574 bp

ATGGTTAGGTCTTTCCCAAGTGATGGCTGGAGTGCCCTGCCTACCCGTTTTGGGTTCTGGCCAGAGAATCCAACCACCGCCGGAGTGGTCAGCAGTCGTGTTTTCTCAGCCAGACTTCCGGAGGTTTGCCGACAGGTTCACGATAAGCAGCCGTTCGGAATGTTCAAAGGCGAAAATGGCATGAACTACACATTTTCAACCTTTTTGATCGAAGCAATACTCATCATTTTCTTCATCAAAATTGTTTACGTCCTCCTTCGACCTCTTCGCCAACCTCGTATAGTCTGCGAGATCATTGGTGGGATGATGATAGGACCGTCCATGTTAGGAAGAAACCGAAACTTCAATTACTATCTGTTTCCACCGATTGCGAATTACATATGCGCAAACATAGGACTGATGGGATTCTTTTACTTCTTCTTCCTCACGGCTGCCAAAACAGACGTTGCAGAAATCTTTAAAGCACCAAGAAAGCATAAGTACATTGCTGCAGTTAGCGTTTTGGTGCCGATTGCTTGCGTGGGGAGCACGGGAGCTGCCCTAAAACACAAAATGGATATAAGATTGCAGAAACCTTCGTCCATTGGAGGGGTCACGTTCGCCTTGGGTTTCACTTCTTTTCCGGTCATTTACACTGTTTTGAGAGACATGAACCTTCTCAACTCTGAGATAGGAAAGTTCGCCATGTCAGTGACTCTCCTTGGAGACATGGTTGGGGTCTACGTGCTTGTTTTGTTTGAAGCCATGGCGCAGGCTGACGGAGGAGGAGGAGCATACTCTGTCATATGGTTTCTCATATCGGCGGCTATCATGGCCGCTTGTTTGCTTTTGGTTGTGAAACGATCCTTTGAATGGATTGTCGCCAAAACGCCTGAGGGAGGACTCGTGAACCAAAACTACATCGTCAATATTCTCATGGGTGTCCTTGTCTCTTGTTTCCTCACTGACATGTTCGGTATGGCTATAGCTGTTGGACCCATTTGGCTGGGGTTGGTGGTCCCTCATGGACCACCGTTAGGTTCGACGTTGGCTATTAGGAGCGAGACCTTTGTTAATGAATTCTTGATGCCTTTCTCTTTTGCTTTGGTGGGACAAAAAACCAATGTCAACTTAATTAGCAAAGAGACGTGGCCCAAACAGATCTCTCCTCTTATCTACATGAGCATCGTTGGCTTCGTCACCAAGTTTGTCTCTTCCACTGGCGCTGCTCTCTTCTTTAAAGTCCCCACTAGGGACAGTCTCACGCTTGGCCTGATGATGAACTTGAGAGGCCAGATCGATATCTTACTCTACTTGCATTGGATCGACAAACAAATGGTAGGCTTACCCGGTTATTCTGTCATGGTTTTGTATGCGATCGTGGTCACGGGTGTAACAGCGCCGCTAATCAGCTTCCTCTACGATCCCACTCGGCCTTATAGAAGCAGCAAGCGCCGCACAATCCAACACACTCCTCAGAACACCGAGACGGGACTAGTTCTTGCAGTAACCGACCATGATACCTTCTCAGGCTTGATCACGTTTCTAGACTTCGCTTATCCCACTAAAACTAGTCCTTTCTCTGTATTCGCAATTCAGCTAGTGGAACTGGAAGGGCGTGCCCAACCCTTGTTTATTGCACACGACAAGAAAAGGGAGGAAGAATACGAGGAAGAGGAAGAGCCTGCAGAGCGTATGGGGAGCAGGCGAGTAGACCAAGTGCAGAGCGCATTTAAACTGTACCAAGAGAAAAGAAGTGAGTGTGTGACGATGCATGCCTACACTGCACATGCTTCAAAGCATAACATGTATCAAAATATTTGCGAGCTGGCTTTGACCAAGAAAACCGCTTTCATTCTCTTACCTTATCAGAAAGAGCGTCTTCAAGACGCAGCTCTTACCGAGCTACGTGACTCCGGCATGCTATCTGTGAACGCAGACGTCTTGGCACACACACCATGTTCTGTCTGTATCTATTACGAGAAGGGACGACTTAAGAACGCTATGGTTAGGTCATCCATGGACCCACAACACACAACAAATTCTAGCCATATGAGGCAAGAAATGTACCGTTTCGTGGTTCTGTTTTTAGGAGGAGCTGATAACAGAGAAGCTCTGCATCTGGCTGATAGGATGACGGAGAATCCGTTCATAAACTTGACAGTGATTAGGTTCTTGGCGCACAACCACGAAGGAGAGGACGAGAGGGAGAAGAAGCTGGACGATGGGGTGGTGACGTGGTTCTGGGTCAAGAACGAAAGCAACGCTAGGGTTAGCTACAAGGAGGTTGTTGTGAAGAACGGTGCTGAGACGTTGGCTGCGATTCAAGCAATGAATGTCAATGACTATGATTTGTGGATAACCGGGAGGAGAGAAGGGATCAATCCAAAGATTCTGGAAGGACTTTCTACGTGGAGCGAAGACCACCAGCTTGGAGTCATTGGAGACACCGTGGCTGGGAGCGTTTTCGCCTCGGAGGGTTCGGTGTTGGTGGTGCAGCAACAAGTGAGAAACCAGATGGGTGGTGATGGTTTCTTGAACGGCAAGTTTGATTACAAGAAATTAGTGTCTCCTTGGTCTCACTCTCATAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

857

Amino Acids

95.83

Weight (kDa)

8.32

Isoelectric Point (pI)

41.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	74 - 462	2.5e-35	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	523 - 661	4.9e-08	Plant cation/H(+) antiporter domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010237)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37060 AT5G58460
fragaria_vesca	FvH4_3g39730
malus_domestica	MD03G1056200.v1.1 MD11G1057100.v1.1 MD11G1057400.v1.1
prunus_persica	Prupe.6G048600_v2.0.a1
pyrus_communis	pycom11g04800 pycom11g04810
rosa_chinensis	RchiOBHm_Chr5g0071611
rosa_laevigata	RLG00000036229
rosa_multiflora	Rmu_sc0005745.1_g000024
rosa_roxburghii	Rroxscaffold_1G00009570
rosa_rugosa	Rorug05G0413600 Rorug05G0413600 Rorug05G0413600
rosa_samantha	Rh5AG469500 Rh5BG488900 Rh5CG512800 Rh5DG499500
rosa_wichuraiana	Rw5G043620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1923
Acc16I	TGCGCA	1 cut(s)	385
AccB1I	GGYRCC	1 cut(s)	505
AccB7I	CCANNNNNTGG	1 cut(s)	23
AccI	GTMKAC	2 cut(s)	720, 1692
AccIII	TCCGGA	1 cut(s)	118
AciI	CCGC	6 cut(s)	78, 803, 816, 1382, 1435, 1827
AclWI	GGATC	4 cut(s)	837, 1310, 1397, 2366
AcoI	YGGCCR	2 cut(s)	59, 813
AcsI	RAATTY	2 cut(s)	1058, 2020
AcyI	GRCGYC	1 cut(s)	1920
AfaI	GTAC	3 cut(s)	482, 1721, 2048
AfiI	CCNNNNNNNGG	9 cut(s)	23, 51, 588, 852, 878, 1016, 1231, 2056, 2436
AflII	CTTAAG	1 cut(s)	1973
AflIII	ACRYGT	2 cut(s)	948, 1785
AgsI	TTSAA	9 cut(s)	166, 198, 346, 740, 851, 1776, 1865, 2306, 2518
AhlI	ACTAGT	2 cut(s)	1474, 1550
AjiI	CACGTC	2 cut(s)	1128, 2217
AloI	GAACNNNNNNTCC	2 cut(s)	2111, 2143
Alw21I	GWGCWC	1 cut(s)	530
AlwI	GGATC	4 cut(s)	837, 1310, 1397, 2366
Aor13HI	TCCGGA	1 cut(s)	118
AoxI	GGCC	6 cut(s)	59, 813, 1130, 1249, 1270, 1412
ApeKI	GCWGC	9 cut(s)	148, 431, 488, 536, 1205, 1425, 1871, 2297, 2476
ApoI	RAATTY	2 cut(s)	1058, 2020
Asp700I	GAANNNNTTC	1 cut(s)	2126
AspLEI	GCGC	7 cut(s)	386, 751, 1205, 1381, 1434, 1709, 2161
AspS9I	GGNCC	6 cut(s)	314, 974, 997, 1007, 1131, 2002
AsuC2I	CCSGG	2 cut(s)	1329, 2344
AsuHPI	GGTGA	3 cut(s)	1171, 2224, 2516
AvaII	GGWCC	5 cut(s)	314, 974, 997, 1007, 2002
AxyI	CCTNAGG	1 cut(s)	873
BaeGI	GKGCMC	2 cut(s)	37, 1603
BalI	TGGCCA	1 cut(s)	61
BanI	GGYRCC	1 cut(s)	505
BauI	CACGAG	1 cut(s)	884
BbsI	GAAGAC	2 cut(s)	1853, 2406
Bbv12I	GWGCWC	1 cut(s)	530
BbvI	GCAGC	9 cut(s)	160, 418, 475, 523, 1192, 1437, 1883, 2284, 2488
BccI	CCATC	5 cut(s)	17, 394, 2198, 2491, 2501
BceAI	ACGGC	3 cut(s)	136, 444, 2536
BclI	TGATCA	1 cut(s)	1515
BcnI	CCSGG	2 cut(s)	1329, 2344
BcuI	ACTAGT	2 cut(s)	1474, 1550
BfaI	CTAG	7 cut(s)	1230, 1475, 1526, 1551, 1580, 2025, 2247
BfmI	CTRYAG	3 cut(s)	489, 963, 1665
BfoI	RGCGCY	3 cut(s)	1206, 1382, 1435
BfrI	CTTAAG	1 cut(s)	1973
BglII	AGATCT	1 cut(s)	1140
Bme1390I	CCNGG	2 cut(s)	1329, 2344
Bme18I	GGWCC	5 cut(s)	314, 974, 997, 1007, 2002
BmgBI	CACGTC	2 cut(s)	1128, 2217
BmgT120I	GGNCC	6 cut(s)	314, 974, 997, 1007, 1131, 2002
BmiI	GGNNCC	4 cut(s)	507, 976, 999, 2004
BmrFI	CCNGG	2 cut(s)	1329, 2344
BmsI	GCATC	4 cut(s)	1056, 1173, 1740, 2107
BpiI	GAAGAC	2 cut(s)	1853, 2406
BpmI	CTGGAG	1 cut(s)	49
BpuEI	CTTGAG	1 cut(s)	1285
BpuMI	CCSGG	2 cut(s)	1329, 2344
Bsa29I	ATCGAT	1 cut(s)	1278
BsaAI	YACGTR	3 cut(s)	724, 1889, 2391
BsaBI	GATNNNNATC	1 cut(s)	1280
BsaHI	GRCGYC	1 cut(s)	1920
BsaI	GGTCTC	2 cut(s)	1037, 2562
BsaJI	CCNNGG	7 cut(s)	603, 700, 744, 1998, 2430, 2451, 2552
BsaWI	WCCGGW	2 cut(s)	118, 622
BsaXI	ACNNNNNCTCC	8 cut(s)	696, 726, 870, 900, 2111, 2141, 2415, 2445
Bsc4I	CCNNNNNNNGG	9 cut(s)	23, 51, 588, 852, 878, 1016, 1231, 2056, 2436
Bse1I	ACTGG	2 cut(s)	1204, 1593
Bse21I	CCTNAGG	1 cut(s)	873
Bse3DI	GCAATG	2 cut(s)	483, 2316
Bse8I	GATNNNNATC	1 cut(s)	1280
BseAI	TCCGGA	1 cut(s)	118
BseCI	ATCGAT	1 cut(s)	1278
BseDI	CCNNGG	7 cut(s)	603, 700, 744, 1998, 2430, 2451, 2552
BseGI	GGATG	3 cut(s)	309, 1994, 2118
BseJI	GATNNNNATC	1 cut(s)	1280
BseLI	CCNNNNNNNGG	9 cut(s)	23, 51, 588, 852, 878, 1016, 1231, 2056, 2436
BseMI	GCAATG	2 cut(s)	483, 2316
BseMII	CTCAG	6 cut(s)	120, 657, 864, 1469, 1521, 2277
BseNI	ACTGG	2 cut(s)	1204, 1593
BseRI	GAGGAG	7 cut(s)	777, 780, 783, 1137, 1443, 2088, 2362
BseSI	GKGCMC	2 cut(s)	37, 1603
BseXI	GCAGC	9 cut(s)	160, 418, 475, 523, 1192, 1437, 1883, 2284, 2488
BseYI	CCCAGC	2 cut(s)	985, 2435
BsgI	GTGCAG	3 cut(s)	1721, 1749, 2495
Bsh1285I	CGRYCG	1 cut(s)	1359
BshFI	GGCC	6 cut(s)	61, 815, 1132, 1251, 1272, 1414
BshNI	GGYRCC	1 cut(s)	505
BshVI	ATCGAT	1 cut(s)	1278
BsiEI	CGRYCG	1 cut(s)	1359
BsiHKAI	GWGCWC	1 cut(s)	530
BsiSI	CCGG	6 cut(s)	81, 119, 623, 1329, 1896, 2343
BslFI	GGGAC	6 cut(s)	983, 1104, 1208, 1247, 1485, 1980
BslI	CCNNNNNNNGG	9 cut(s)	23, 51, 588, 852, 878, 1016, 1231, 2056, 2436
BsmBI	CGTCTC	3 cut(s)	1118, 1460, 2282
BsmFI	GGGAC	6 cut(s)	983, 1104, 1208, 1247, 1485, 1980
BsnI	GGCC	6 cut(s)	61, 815, 1132, 1251, 1272, 1414
Bso31I	GGTCTC	2 cut(s)	1037, 2562
Bsp1286I	GDGCHC	3 cut(s)	37, 530, 1603
Bsp13I	TCCGGA	1 cut(s)	118
Bsp19I	CCATGG	2 cut(s)	744, 1998
BspACI	CCGC	6 cut(s)	78, 803, 816, 1382, 1435, 1827
BspANI	GGCC	6 cut(s)	61, 815, 1132, 1251, 1272, 1414
BspCNI	CTCAG	6 cut(s)	119, 658, 865, 1468, 1520, 2278
BspDI	ATCGAT	1 cut(s)	1278
BspEI	TCCGGA	1 cut(s)	118
BspLI	GGNNCC	4 cut(s)	507, 976, 999, 2004
BspMAI	CTGCAG	2 cut(s)	493, 1669
BspPI	GGATC	4 cut(s)	837, 1310, 1397, 2366
BspQI	GCTCTTC	1 cut(s)	1653
BspT107I	GGYRCC	1 cut(s)	505
BspTI	CTTAAG	1 cut(s)	1973
BspTNI	GGTCTC	2 cut(s)	1037, 2562
BsrDI	GCAATG	2 cut(s)	483, 2316
BsrI	ACTGG	2 cut(s)	1204, 1593
BssECI	CCNNGG	7 cut(s)	603, 700, 744, 1998, 2430, 2451, 2552
BssNI	GRCGYC	1 cut(s)	1920
BssSI	CACGAG	1 cut(s)	884
BssT1I	CCWWGG	5 cut(s)	603, 700, 744, 1998, 2552
Bst2BI	CACGAG	1 cut(s)	884
Bst4CI	ACNGT	9 cut(s)	318, 637, 1014, 1238, 1719, 2051, 2143, 2282, 2431
Bst6I	CTCTTC	5 cut(s)	270, 1198, 1217, 1647, 1653
BstACI	GRCGYC	1 cut(s)	1920
BstAFI	CTTAAG	1 cut(s)	1973
BstBAI	YACGTR	3 cut(s)	724, 1889, 2391
BstDEI	CTNAG	6 cut(s)	106, 666, 873, 1455, 1507, 2286
BstDSI	CCRYGG	3 cut(s)	744, 1998, 2430
BstENI	CCTNNNNNAGG	1 cut(s)	876
BstF5I	GGATG	3 cut(s)	309, 1994, 2118
BstH2I	RGCGCY	3 cut(s)	1206, 1382, 1435
BstHHI	GCGC	7 cut(s)	386, 751, 1205, 1381, 1434, 1709, 2161
BstMCI	CGRYCG	1 cut(s)	1359
BstMWI	GCNNNNNNNGC	9 cut(s)	177, 525, 812, 1170, 1431, 1434, 1669, 1763, 2306
BstNSI	RCATGY	5 cut(s)	952, 1757, 1772, 1789, 1903
BstSCI	CCNGG	2 cut(s)	1327, 2342
BstSFI	CTRYAG	3 cut(s)	489, 963, 1665
BstSLI	GKGCMC	2 cut(s)	37, 1603
BstV1I	GCAGC	9 cut(s)	160, 418, 475, 523, 1192, 1437, 1883, 2284, 2488
BstV2I	GAAGAC	2 cut(s)	1853, 2406
BstX2I	RGATCY	1 cut(s)	1140
BstXI	CCANNNNNNTGG	3 cut(s)	985, 2411, 2501
BstYI	RGATCY	1 cut(s)	1140
Bsu15I	ATCGAT	1 cut(s)	1278
Bsu36I	CCTNAGG	1 cut(s)	873
BsuRI	GGCC	6 cut(s)	61, 815, 1132, 1251, 1272, 1414
BsuTUI	ATCGAT	1 cut(s)	1278
BtgI	CCRYGG	3 cut(s)	744, 1998, 2430
BtrI	CACGTC	2 cut(s)	1128, 2217
BtsCI	GGATG	3 cut(s)	309, 1994, 2118
BtsI	GCAGTG	1 cut(s)	1761
BtsIMutI	CAGTG	6 cut(s)	633, 696, 942, 1197, 1761, 2148
CfoI	GCGC	7 cut(s)	386, 751, 1205, 1381, 1434, 1709, 2161
Cfr13I	GGNCC	6 cut(s)	314, 974, 997, 1007, 1131, 2002
ClaI	ATCGAT	1 cut(s)	1278
CseI	GACGC	2 cut(s)	1847, 1877
Csp6I	GTAC	3 cut(s)	481, 1720, 2047
CviQI	GTAC	3 cut(s)	481, 1720, 2047
DdeI	CTNAG	6 cut(s)	106, 666, 873, 1455, 1507, 2286
DraI	TTTAAA	3 cut(s)	460, 1219, 1714
EaeI	YGGCCR	2 cut(s)	59, 813
Eam1104I	CTCTTC	5 cut(s)	270, 1198, 1217, 1647, 1653
EarI	CTCTTC	5 cut(s)	270, 1198, 1217, 1647, 1653
Eco130I	CCWWGG	5 cut(s)	603, 700, 744, 1998, 2552
Eco31I	GGTCTC	2 cut(s)	1037, 2562
Eco32I	GATATC	1 cut(s)	1282
Eco47I	GGWCC	5 cut(s)	314, 974, 997, 1007, 2002
Eco81I	CCTNAGG	1 cut(s)	873
EcoNI	CCTNNNNNAGG	1 cut(s)	876
EcoRI	GAATTC	1 cut(s)	1058
EcoRV	GATATC	1 cut(s)	1282
EcoT14I	CCWWGG	5 cut(s)	603, 700, 744, 1998, 2552
EcoT22I	ATGCAT	1 cut(s)	1755
ErhI	CCWWGG	5 cut(s)	603, 700, 744, 1998, 2552
Esp3I	CGTCTC	3 cut(s)	1118, 1460, 2282
FalI	AAGNNNNNCTT	2 cut(s)	1845, 1877
FaqI	GGGAC	6 cut(s)	983, 1104, 1208, 1247, 1485, 1980
FauNDI	CATATG	3 cut(s)	380, 785, 2031
FbaI	TGATCA	1 cut(s)	1515
FblI	GTMKAC	2 cut(s)	720, 1692
FokI	GGATG	3 cut(s)	316, 1981, 2125
FspBI	CTAG	7 cut(s)	1230, 1475, 1526, 1551, 1580, 2025, 2247
FspI	TGCGCA	1 cut(s)	385
GlaI	GCGC	7 cut(s)	385, 750, 1204, 1380, 1433, 1708, 2160
GsaI	CCCAGC	2 cut(s)	989, 2439
GsuI	CTGGAG	1 cut(s)	49
HaeII	RGCGCY	3 cut(s)	1206, 1382, 1435
HaeIII	GGCC	6 cut(s)	61, 815, 1132, 1251, 1272, 1414
HapII	CCGG	6 cut(s)	81, 119, 623, 1329, 1896, 2343
HgaI	GACGC	2 cut(s)	1847, 1877
HhaI	GCGC	7 cut(s)	386, 751, 1205, 1381, 1434, 1709, 2161
Hin1I	GRCGYC	1 cut(s)	1920
Hin6I	GCGC	7 cut(s)	384, 749, 1203, 1379, 1432, 1707, 2159
HinP1I	GCGC	7 cut(s)	384, 749, 1203, 1379, 1432, 1707, 2159
HincII	GTYRAC	1 cut(s)	1108
HindII	GTYRAC	1 cut(s)	1108
HinfI	GANTC	9 cut(s)	67, 405, 694, 882, 1892, 2122, 2302, 2371, 2415
HpaII	CCGG	6 cut(s)	81, 119, 623, 1329, 1896, 2343
HphI	GGTGA	3 cut(s)	1171, 2224, 2516
Hpy166II	GTNNAC	7 cut(s)	139, 247, 721, 889, 1108, 1693, 1912
Hpy188I	TCNGA	5 cut(s)	158, 667, 1458, 1851, 2455
Hpy188III	TCNNGA	9 cut(s)	119, 140, 886, 1063, 1526, 1865, 2230, 2375, 2515
Hpy8I	GTNNAC	7 cut(s)	139, 247, 721, 889, 1108, 1693, 1912
Hpy99I	CGWCG	1 cut(s)	1027
HpyAV	CCTTC	9 cut(s)	266, 585, 665, 1513, 1586, 1957, 2165, 2348, 2372
HpyCH4III	ACNGT	9 cut(s)	318, 637, 1014, 1238, 1719, 2051, 2143, 2282, 2431
HpyF10VI	GCNNNNNNNGC	9 cut(s)	177, 525, 812, 1170, 1431, 1434, 1669, 1763, 2306
HpyF3I	CTNAG	6 cut(s)	106, 666, 873, 1455, 1507, 2286
Hsp92I	GRCGYC	1 cut(s)	1920
HspAI	GCGC	7 cut(s)	384, 749, 1203, 1379, 1432, 1707, 2159
Kpn2I	TCCGGA	1 cut(s)	118
Ksp22I	TGATCA	1 cut(s)	1515
LguI	GCTCTTC	1 cut(s)	1653
LmnI	GCTCC	8 cut(s)	525, 533, 770, 1038, 1680, 2075, 2394, 2439
Lsp1109I	GCAGC	9 cut(s)	160, 418, 475, 523, 1192, 1437, 1883, 2284, 2488
LweI	GCATC	4 cut(s)	1056, 1173, 1740, 2107
MaeI	CTAG	7 cut(s)	1230, 1475, 1526, 1551, 1580, 2025, 2247
MaeIII	GTNAC	9 cut(s)	592, 691, 1177, 1363, 1372, 1486, 1744, 1889, 2212
MflI	RGATCY	1 cut(s)	1140
MhlI	GDGCHC	3 cut(s)	37, 530, 1603
MlsI	TGGCCA	1 cut(s)	61
MluCI	AATT	9 cut(s)	240, 346, 373, 1058, 1113, 1572, 2020, 2540, 2569
MluNI	TGGCCA	1 cut(s)	61
MlyI	GAGTC	4 cut(s)	688, 876, 1886, 2424
MmeI	TCCRAC	3 cut(s)	95, 952, 1468
Mox20I	TGGCCA	1 cut(s)	61
Mph1103I	ATGCAT	1 cut(s)	1755
MroI	TCCGGA	1 cut(s)	118
MroXI	GAANNNNTTC	1 cut(s)	2126
MscI	TGGCCA	1 cut(s)	61
MseI	TTAA	6 cut(s)	459, 1053, 1112, 1218, 1713, 1974
MslI	CAYNNNNRTG	2 cut(s)	689, 947
Msp20I	TGGCCA	1 cut(s)	61
MspCI	CTTAAG	1 cut(s)	1973
MspI	CCGG	6 cut(s)	81, 119, 623, 1329, 1896, 2343
MspR9I	CCNGG	2 cut(s)	1329, 2344
MwoI	GCNNNNNNNGC	9 cut(s)	177, 525, 812, 1170, 1431, 1434, 1669, 1763, 2306
NciI	CCSGG	2 cut(s)	1329, 2344
NcoI	CCATGG	2 cut(s)	744, 1998
NdeI	CATATG	3 cut(s)	380, 785, 2031
NlaIV	GGNNCC	4 cut(s)	507, 976, 999, 2004
NmeAIII	GCCGAG	1 cut(s)	1390
NmuCI	GTSAC	7 cut(s)	592, 691, 1177, 1363, 1744, 1889, 2212
NsbI	TGCGCA	1 cut(s)	385
NsiI	ATGCAT	1 cut(s)	1755
NspI	RCATGY	5 cut(s)	952, 1757, 1772, 1789, 1903
PaeI	GCATGC	2 cut(s)	1757, 1903
PciI	ACATGT	2 cut(s)	948, 1785
PciSI	GCTCTTC	1 cut(s)	1653
PcsI	WCGNNNNNNNCGW	4 cut(s)	274, 1173, 2123, 2297
PdmI	GAANNNNTTC	1 cut(s)	2126
PfeI	GAWTC	5 cut(s)	67, 405, 2122, 2302, 2371
PflMI	CCANNNNNTGG	1 cut(s)	23
Ple19I	CGATCG	1 cut(s)	1359
PleI	GAGTC	4 cut(s)	688, 876, 1886, 2423
PpsI	GAGTC	4 cut(s)	688, 876, 1886, 2423
Ppu21I	YACGTR	3 cut(s)	724, 1889, 2391
PscI	ACATGT	2 cut(s)	948, 1785
PspFI	CCCAGC	2 cut(s)	985, 2435
PspN4I	GGNNCC	4 cut(s)	507, 976, 999, 2004
PspPI	GGNCC	6 cut(s)	314, 974, 997, 1007, 1131, 2002
PsrI	GAACNNNNNNTAC	2 cut(s)	881, 913
PstI	CTGCAG	2 cut(s)	493, 1669
PsuI	RGATCY	1 cut(s)	1140
PvuI	CGATCG	1 cut(s)	1359
RsaI	GTAC	3 cut(s)	482, 1721, 2048
RsaNI	GTAC	3 cut(s)	481, 1720, 2047
RseI	CAYNNNNRTG	2 cut(s)	689, 947
SapI	GCTCTTC	1 cut(s)	1653
SaqAI	TTAA	6 cut(s)	459, 1053, 1112, 1218, 1713, 1974
Sau96I	GGNCC	6 cut(s)	314, 974, 997, 1007, 1131, 2002
SchI	GAGTC	4 cut(s)	688, 876, 1886, 2424
ScrFI	CCNGG	2 cut(s)	1329, 2344
SduI	GDGCHC	3 cut(s)	37, 530, 1603
SfaNI	GCATC	4 cut(s)	1056, 1173, 1740, 2107
SfcI	CTRYAG	3 cut(s)	489, 963, 1665
SinI	GGWCC	5 cut(s)	314, 974, 997, 1007, 2002
SmiMI	CAYNNNNRTG	2 cut(s)	689, 947
SmlI	CTYRAG	2 cut(s)	1264, 1973
SmoI	CTYRAG	2 cut(s)	1264, 1973
SpeI	ACTAGT	2 cut(s)	1474, 1550
SphI	GCATGC	2 cut(s)	1757, 1903
Sse9I	AATT	9 cut(s)	240, 346, 373, 1058, 1113, 1572, 2020, 2540, 2569
SsiI	CCGC	6 cut(s)	78, 803, 816, 1382, 1435, 1827
SspI	AATATT	2 cut(s)	910, 1798
SspMI	CTAG	7 cut(s)	1230, 1475, 1526, 1551, 1580, 2025, 2247
StyD4I	CCNGG	2 cut(s)	1327, 2342
StyI	CCWWGG	5 cut(s)	603, 700, 744, 1998, 2552
TaaI	ACNGT	9 cut(s)	318, 637, 1014, 1238, 1719, 2051, 2143, 2282, 2431
TaqI	TCGA	5 cut(s)	210, 259, 1022, 1278, 1305
TasI	AATT	9 cut(s)	240, 346, 373, 1058, 1113, 1572, 2020, 2540, 2569
TatI	WGTACW	1 cut(s)	480
TauI	GCSGC	4 cut(s)	806, 818, 1384, 1437
TfiI	GAWTC	5 cut(s)	67, 405, 2122, 2302, 2371
Tru1I	TTAA	6 cut(s)	459, 1053, 1112, 1218, 1713, 1974
Tru9I	TTAA	6 cut(s)	459, 1053, 1112, 1218, 1713, 1974
TscAI	CASTG	6 cut(s)	640, 696, 949, 1204, 1768, 2148
TseFI	GTSAC	7 cut(s)	592, 691, 1177, 1363, 1744, 1889, 2212
TseI	GCWGC	9 cut(s)	148, 431, 488, 536, 1205, 1425, 1871, 2297, 2476
Tsp45I	GTSAC	7 cut(s)	592, 691, 1177, 1363, 1744, 1889, 2212
TspDTI	ATGAA	8 cut(s)	197, 223, 665, 1071, 1274, 1822, 2119, 2327
TspGWI	ACGGA	3 cut(s)	774, 2115, 2132
TspRI	CASTG	6 cut(s)	640, 696, 949, 1204, 1768, 2148
Van91I	CCANNNNNTGG	1 cut(s)	23
Vha464I	CTTAAG	1 cut(s)	1973
VpaK11BI	GGWCC	5 cut(s)	314, 974, 997, 1007, 2002
XagI	CCTNNNNNAGG	1 cut(s)	876
XapI	RAATTY	2 cut(s)	1058, 2020
XbaI	TCTAGA	1 cut(s)	1525
XceI	RCATGY	5 cut(s)	952, 1757, 1772, 1789, 1903
XcmI	CCANNNNNNNNNTGG	2 cut(s)	24, 82
XmiI	GTMKAC	2 cut(s)	720, 1692
XmnI	GAANNNNTTC	1 cut(s)	2126
XspI	CTAG	7 cut(s)	1230, 1475, 1526, 1551, 1580, 2025, 2247
ZraI	GACGTC	1 cut(s)	1921
Zsp2I	ATGCAT	1 cut(s)	1755

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.