Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0010237

Physical Location & Seq

Genomic Coordinates Forward (+)

4876330 .. 4880171

Gene Structure 3842 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1057400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2532 bp

ATGGTTAGGAAATTCCCACTTTACCTTCCCCCAACTCCTTGGGATGGAGGTTCAAACAGATTTCAGGCGTCCGTGAAAGCTCAGGCCAGCAATGTGTCCATAGATTTCAACAATGTTTATCCTTCAATCATAATTTCATGTGGAAAGACTCGACAGACAGGCTTCTACAGCTTACTTTACGGTGAAAATCCATTGAAAAGTTCATTCACTCTTTTGTTGTTAGAGTTCGCTTTGATCATCGTCCTCACACGTATCCTCCGTGTTCTTCTCAAGCCTCTCAAACAACCCAGAATGGTCTCAGAAGTCATTGGTGGTATATTCATTGGACCGTCAATTTTAGGCCGCAGCGAGAAATTTACTTCAGTTATGTTTCCGAAAAATTCAAAATTCATAGTGAGAAATATTGGATTAATTGGATTCACTTATTATTTTTTCTTGTCCGGAGTGAAAATGGACCTTTCGCTGGTGAAAAAGACAAATAAGAAACAATTGTACATGGCATTTGCTGGAGTGCTCCTCCCCTTCATAGTTATATCAATTGTTGCATTCAGTTTGCGCAAGTCCATGGACAAAGAACTAGCTAGAATTTCTTCACTTGGATTCATTTCCTCCGGCTTGGCCCTACCGTTGTTTCCGGTCATCCACGCCATCCTCAAAGAGCTCAACCTTCTGAGCTCTGAGATCGGACGTCTGGCATTGTCGATATCGGTGATCAGCGATGCGATTGGAGGCTGTATAATGGTTTTATTTGAGGCAGCTAAGCAAGGTGAGGGCAACCACCTGGCTGTGTTATGGTACTTGATTTCTGTGGTTGTTTTTGTGGCCTTCATTGTGTTTGTGATTAGAAGGAGTTTGTTGAGAGTTGCTGAGACCACCCCAGAAGGGCAACCAGTGGACCAAGCATACGTGGTGTCGATTTTGTTGGGGGTTTTAGTGATGGGATGTTTGGGTGACATCTCTGGGATCACGATCGTAAACGGTGCGTTCTGGTTGGGTTTCGCGATCCCGGATGGCCCTCCGCTTGGATCAACAATTGTAGAGAGGAGTGAGATGGTTATAGTGGATGTTTTGATGCCATTTTCTTTTGCAATGGTTGGGCTTTCTGTGGATGTGAATGCCATGAGCTCTGTTGGTTGGTCAAGCTTGAGTCCCTTGTTTGCCATCACTGCGACTGCCTATGTGGCTAAGTTGCTTGGAACCCTAATCACTTCTGCCTTCTTTGAGTTGCCACTCAGAGATGGTGTTACGCTCAGCTTCATGATGATCCTAAAAGGTCAAGTGGAGATAGTGGTTTTACTCCATTGGATGGACAAAAAGATAATGGCGATTCCAGGGTTTACAATGATGGTGATATCCATCACTACAATGACTGCAATAGCCACGCCCTTGATCAGCATCCTCTATGACCCAACAAGGCCATACATGGTCCATAAAAGGAGAACCATCCAGCACACTCCTCTGGAGGAAGCAGAACTTCGCATAGTCCTATGTATATATGATGAAGATAGCATAGCTGGCCTCATAAACCTTCTTGAAATCTCCAATCCAACTCTAAGCACCCCGTTCGTAATCTTTTCTCTCCATCTTGTCGACCTTGTTGGCCGGGCTTGTCCTGTGCTTATAGACCATGAAAAGCAGGAAGAGCAAGATTCAAAATATGCAGTTTGTCACACAATCCACAATGTCATAAAGCAATACCAAGAAACCAAAGGTGAGTGTGTTAGGCTCCATCCCTTCACAGCTATAGTGCCAAACAAAACAATGTACCAAGACATTTGTGACCTAGCTCTTGTGAAAAAGGCCACACTTTTAATTTTGCCTTTTCACAAAGAATGTTTGGATACTCTTGGAGGACAATTAACAGAAACTGTGAGGCATGGGGTTAGATCTGTCAATTCAAATGTGTTAGCCCATGCACCTTGCTCAGTTGGAGTTTTGGTTGACAAGGGTCATGTTAACCAACTGGCATTAAGGAACACCGAGCTACACTTTGCCGTGCTATATTTGGGCGGAGCAGATTCTAGGGAGGCATTGTGTTATGCAGATAGGATGGCTGGAAAATTCAATGTGTCACTGACTGTGATTCGGTTTCTTTCACACAATTCTGAAGGTGATGATGAGATGGAGAAGAAGTTGGATGATGGGGTTGTGACATGGTTTTGGATGAAGAATGAGAGGAATGAGAGGGTGAATTATAAAGAGGTGGTGGTGAGGAATGGGGAAGAGACAATCTCAGCCATTCAATCAATGAATGATGATAATGAAAATAATTATGACCTTTGGATTGTGGGGAGGAAACAAGGGATCAATCCTGTGCTTTTATCTGGGCTATCATGTTGGAGTGAGAATGGTGAGTTGGGAATAATTGGGGACTATATTTCCTCTCTTGATTTTTGTTGTACTGCTTCTGTGTTAGTGGTGCAACAGCAGACTTTGAGAGGGCAAGGGTCAGATAGTACAAGTGGAAGGTTTGCATGTAACCCTAGCCCTACTCAGAAGGTGAAGGATTTGTTAATTTCATGGTGTTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

844

Amino Acids

93.18

Weight (kDa)

6.96

Isoelectric Point (pI)

41.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	80 - 466	4.3e-38	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	522 - 651	1.1e-10	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	663 - 811	9.2e-10	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010237)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37060 AT5G58460
fragaria_vesca	FvH4_3g39730
malus_domestica	MD03G1056200.v1.1 MD11G1057100.v1.1 MD11G1057400.v1.1
prunus_persica	Prupe.6G048600_v2.0.a1
pyrus_communis	pycom11g04800 pycom11g04810
rosa_chinensis	RchiOBHm_Chr5g0071611
rosa_laevigata	RLG00000036229
rosa_multiflora	Rmu_sc0005745.1_g000024
rosa_roxburghii	Rroxscaffold_1G00009570
rosa_rugosa	Rorug05G0413600 Rorug05G0413600 Rorug05G0413600
rosa_samantha	Rh5AG469500 Rh5BG488900 Rh5CG512800 Rh5DG499500
rosa_wichuraiana	Rw5G043620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2196
AatII	GACGTC	1 cut(s)	691
Acc16I	TGCGCA	1 cut(s)	557
AccB7I	CCANNNNNTGG	1 cut(s)	1306
AccI	GTMKAC	1 cut(s)	1590
AccII	CGCG	1 cut(s)	1001
AccIII	TCCGGA	1 cut(s)	440
AciI	CCGC	3 cut(s)	343, 1019, 2010
AclWI	GGATC	5 cut(s)	971, 997, 1033, 1258, 2312
AcoI	YGGCCR	1 cut(s)	1600
AcsI	RAATTY	6 cut(s)	11, 353, 379, 386, 585, 2060
AcuI	CTGAAG	2 cut(s)	345, 2127
AcyI	GRCGYC	2 cut(s)	68, 688
AfaI	GTAC	5 cut(s)	494, 797, 1766, 2401, 2458
AfiI	CCNNNNNNNGG	5 cut(s)	44, 463, 882, 1022, 1306
AflIII	ACRYGT	1 cut(s)	248
AjnI	CCWGG	2 cut(s)	780, 1330
AjuI	GAANNNNNNNTTGG	2 cut(s)	2339, 2371
Alw21I	GWGCWC	4 cut(s)	516, 663, 677, 1127
Alw26I	GTCTC	3 cut(s)	301, 863, 2219
AlwI	GGATC	5 cut(s)	971, 997, 1033, 1258, 2312
AlwNI	CAGNNNCTG	1 cut(s)	1868
Aor13HI	TCCGGA	1 cut(s)	440
AoxI	GGCC	9 cut(s)	84, 340, 618, 822, 1012, 1415, 1516, 1600, 1800
ApeKI	GCWGC	2 cut(s)	345, 755
ApoI	RAATTY	6 cut(s)	11, 353, 379, 386, 585, 2060
AseI	ATTAAT	1 cut(s)	410
Asp700I	GAANNNNTTC	1 cut(s)	589
AspLEI	GCGC	1 cut(s)	558
AspS9I	GGNCC	6 cut(s)	326, 454, 619, 895, 1013, 1426
AsuC2I	CCSGG	2 cut(s)	1007, 1604
AvaII	GGWCC	4 cut(s)	326, 454, 895, 1426
BanII	GRGCYC	3 cut(s)	663, 677, 1127
Bbv12I	GWGCWC	4 cut(s)	516, 663, 677, 1127
BbvI	GCAGC	2 cut(s)	357, 767
BceAI	ACGGC	1 cut(s)	1979
BcgI	CGANNNNNNTGC	2 cut(s)	1546, 1580
BciT130I	CCWGG	2 cut(s)	782, 1332
BciVI	GTATCC	2 cut(s)	263, 1834
BclI	TGATCA	3 cut(s)	234, 711, 1389
BcnI	CCSGG	2 cut(s)	1007, 1604
BcoDI	GTCTC	3 cut(s)	301, 863, 2219
BfaI	CTAG	5 cut(s)	578, 582, 1784, 2022, 2484
BfmI	CTRYAG	2 cut(s)	166, 1743
BfuI	GTATCC	2 cut(s)	263, 1834
BglI	GCCNNNNNGGC	1 cut(s)	1181
BglII	AGATCT	1 cut(s)	1886
BisI	GCNGC	3 cut(s)	343, 346, 756
BlpI	GCTNAGC	2 cut(s)	759, 1250
BlsI	GCNGC	3 cut(s)	344, 347, 757
Bme1390I	CCNGG	4 cut(s)	782, 1007, 1332, 1604
Bme18I	GGWCC	4 cut(s)	326, 454, 895, 1426
BmgT120I	GGNCC	6 cut(s)	326, 454, 619, 895, 1013, 1426
BmiI	GGNNCC	2 cut(s)	1198, 1727
BmrFI	CCNGG	4 cut(s)	782, 1007, 1332, 1604
BmsI	GCATC	3 cut(s)	709, 1062, 1404
BoxI	GACNNNNGTC	1 cut(s)	1947
BplI	GAGNNNNNCTC	4 cut(s)	501, 533, 2216, 2248
BpmI	CTGGAG	2 cut(s)	528, 1481
Bpu10I	CCTNAGC	1 cut(s)	81
Bpu1102I	GCTNAGC	2 cut(s)	759, 1250
BpuEI	CTTGAG	2 cut(s)	254, 1165
BpuMI	CCSGG	2 cut(s)	1007, 1604
BsaAI	YACGTR	2 cut(s)	251, 907
BsaBI	GATNNNNATC	3 cut(s)	968, 1355, 1394
BsaHI	GRCGYC	2 cut(s)	68, 688
BsaI	GGTCTC	2 cut(s)	301, 863
BsaJI	CCNNGG	3 cut(s)	38, 564, 1331
BsaWI	WCCGGW	2 cut(s)	440, 634
BsaXI	ACNNNNNCTCC	8 cut(s)	240, 270, 1923, 1953, 2018, 2048, 2117, 2147
Bsc4I	CCNNNNNNNGG	5 cut(s)	44, 463, 882, 1022, 1306
Bse1I	ACTGG	2 cut(s)	890, 1968
Bse3DI	GCAATG	2 cut(s)	97, 1095
Bse8I	GATNNNNATC	3 cut(s)	968, 1355, 1394
BseAI	TCCGGA	1 cut(s)	440
BseBI	CCWGG	2 cut(s)	782, 1332
BseDI	CCNNGG	3 cut(s)	38, 564, 1331
BseJI	GATNNNNATC	3 cut(s)	968, 1355, 1394
BseLI	CCNNNNNNNGG	5 cut(s)	44, 463, 882, 1022, 1306
BseMI	GCAATG	2 cut(s)	97, 1095
BseNI	ACTGG	2 cut(s)	890, 1968
BseRI	GAGGAG	3 cut(s)	506, 1057, 1446
BseXI	GCAGC	2 cut(s)	357, 767
Bsh1236I	CGCG	1 cut(s)	1001
Bsh1285I	CGRYCG	1 cut(s)	972
BshFI	GGCC	9 cut(s)	86, 342, 620, 824, 1014, 1417, 1518, 1602, 1802
BsiEI	CGRYCG	1 cut(s)	972
BsiHKAI	GWGCWC	4 cut(s)	516, 663, 677, 1127
BsiSI	CCGG	5 cut(s)	441, 612, 635, 1007, 1603
BslFI	GGGAC	2 cut(s)	1134, 2384
BslI	CCNNNNNNNGG	5 cut(s)	44, 463, 882, 1022, 1306
BsmAI	GTCTC	3 cut(s)	301, 863, 2219
BsmFI	GGGAC	2 cut(s)	1134, 2384
BsmI	GAATGC	2 cut(s)	545, 1120
BsnI	GGCC	9 cut(s)	86, 342, 620, 824, 1014, 1417, 1518, 1602, 1802
Bso31I	GGTCTC	2 cut(s)	301, 863
Bsp1286I	GDGCHC	4 cut(s)	516, 663, 677, 1127
Bsp13I	TCCGGA	1 cut(s)	440
Bsp1407I	TGTACA	1 cut(s)	492
Bsp1720I	GCTNAGC	2 cut(s)	759, 1250
Bsp19I	CCATGG	1 cut(s)	564
Bsp68I	TCGCGA	1 cut(s)	1001
BspACI	CCGC	3 cut(s)	343, 1019, 2010
BspANI	GGCC	9 cut(s)	86, 342, 620, 824, 1014, 1417, 1518, 1602, 1802
BspEI	TCCGGA	1 cut(s)	440
BspFNI	CGCG	1 cut(s)	1001
BspHI	TCATGA	1 cut(s)	1257
BspLI	GGNNCC	2 cut(s)	1198, 1727
BspPI	GGATC	5 cut(s)	971, 997, 1033, 1258, 2312
BspQI	GCTCTTC	1 cut(s)	1635
BspTNI	GGTCTC	2 cut(s)	301, 863
BsrDI	GCAATG	2 cut(s)	97, 1095
BsrGI	TGTACA	1 cut(s)	492
BsrI	ACTGG	2 cut(s)	890, 1968
BssECI	CCNNGG	3 cut(s)	38, 564, 1331
BssNI	GRCGYC	2 cut(s)	68, 688
BssT1I	CCWWGG	2 cut(s)	38, 564
Bst2UI	CCWGG	2 cut(s)	782, 1332
Bst4CI	ACNGT	6 cut(s)	182, 330, 627, 980, 1870, 2080
Bst6I	CTCTTC	2 cut(s)	1635, 2217
BstACI	GRCGYC	2 cut(s)	68, 688
BstAUI	TGTACA	1 cut(s)	492
BstBAI	YACGTR	2 cut(s)	251, 907
BstC8I	GCNNGC	2 cut(s)	88, 1516
BstDSI	CCRYGG	1 cut(s)	564
BstFNI	CGCG	1 cut(s)	1001
BstHHI	GCGC	1 cut(s)	558
BstMAI	GTCTC	3 cut(s)	301, 863, 2219
BstMCI	CGRYCG	1 cut(s)	972
BstMWI	GCNNNNNNNGC	5 cut(s)	168, 1166, 1181, 1515, 1642
BstNI	CCWGG	2 cut(s)	782, 1332
BstNSI	RCATGY	1 cut(s)	2478
BstPAI	GACNNNNGTC	1 cut(s)	1947
BstSCI	CCNGG	4 cut(s)	780, 1005, 1330, 1602
BstSFI	CTRYAG	2 cut(s)	166, 1743
BstUI	CGCG	1 cut(s)	1001
BstV1I	GCAGC	2 cut(s)	357, 767
BstX2I	RGATCY	1 cut(s)	1886
BstXI	CCANNNNNNTGG	1 cut(s)	39
BstYI	RGATCY	1 cut(s)	1886
BsuI	GTATCC	2 cut(s)	263, 1834
BsuRI	GGCC	9 cut(s)	86, 342, 620, 824, 1014, 1417, 1518, 1602, 1802
BtgI	CCRYGG	1 cut(s)	564
BtgZI	GCGATG	1 cut(s)	732
BtsI	GCAGTG	1 cut(s)	1164
BtsIMutI	CAGTG	3 cut(s)	897, 1164, 2072
BtuMI	TCGCGA	1 cut(s)	1001
Cac8I	GCNNGC	2 cut(s)	88, 1516
CaiI	CAGNNNCTG	1 cut(s)	1868
CciI	TCATGA	1 cut(s)	1257
CfoI	GCGC	1 cut(s)	558
Cfr13I	GGNCC	6 cut(s)	326, 454, 619, 895, 1013, 1426
CseI	GACGC	1 cut(s)	57
Csp6I	GTAC	5 cut(s)	493, 796, 1765, 2400, 2457
CviQI	GTAC	5 cut(s)	493, 796, 1765, 2400, 2457
EaeI	YGGCCR	1 cut(s)	1600
Eam1104I	CTCTTC	2 cut(s)	1635, 2217
EarI	CTCTTC	2 cut(s)	1635, 2217
EciI	GGCGGA	1 cut(s)	2025
Ecl136II	GAGCTC	3 cut(s)	661, 675, 1125
Eco130I	CCWWGG	2 cut(s)	38, 564
Eco24I	GRGCYC	3 cut(s)	663, 677, 1127
Eco31I	GGTCTC	2 cut(s)	301, 863
Eco32I	GATATC	2 cut(s)	705, 1353
Eco47I	GGWCC	4 cut(s)	326, 454, 895, 1426
Eco53kI	GAGCTC	3 cut(s)	661, 675, 1125
Eco57I	CTGAAG	2 cut(s)	345, 2127
EcoICRI	GAGCTC	3 cut(s)	661, 675, 1125
EcoRII	CCWGG	2 cut(s)	780, 1330
EcoRV	GATATC	2 cut(s)	705, 1353
EcoT14I	CCWWGG	2 cut(s)	38, 564
EcoT38I	GRGCYC	3 cut(s)	663, 677, 1127
ErhI	CCWWGG	2 cut(s)	38, 564
FalI	AAGNNNNNCTT	2 cut(s)	1458, 1490
FaqI	GGGAC	2 cut(s)	1134, 2384
FbaI	TGATCA	3 cut(s)	234, 711, 1389
FblI	GTMKAC	1 cut(s)	1590
Fnu4HI	GCNGC	3 cut(s)	343, 346, 756
FriOI	GRGCYC	3 cut(s)	663, 677, 1127
Fsp4HI	GCNGC	3 cut(s)	343, 346, 756
FspBI	CTAG	5 cut(s)	578, 582, 1784, 2022, 2484
FspI	TGCGCA	1 cut(s)	557
GlaI	GCGC	1 cut(s)	557
GluI	GCNGC	3 cut(s)	343, 346, 756
GsuI	CTGGAG	2 cut(s)	528, 1481
HaeIII	GGCC	9 cut(s)	86, 342, 620, 824, 1014, 1417, 1518, 1602, 1802
HapII	CCGG	5 cut(s)	441, 612, 635, 1007, 1603
HgaI	GACGC	1 cut(s)	57
HhaI	GCGC	1 cut(s)	558
Hin1I	GRCGYC	2 cut(s)	68, 688
Hin6I	GCGC	1 cut(s)	556
HinP1I	GCGC	1 cut(s)	556
HincII	GTYRAC	3 cut(s)	1591, 1942, 1957
HindII	GTYRAC	3 cut(s)	1591, 1942, 1957
HindIII	AAGCTT	1 cut(s)	1141
HinfI	GANTC	8 cut(s)	148, 417, 600, 1147, 1327, 1649, 2018, 2083
HpaI	GTTAAC	1 cut(s)	1957
HpaII	CCGG	5 cut(s)	441, 612, 635, 1007, 1603
Hpy166II	GTNNAC	6 cut(s)	895, 976, 1338, 1591, 1942, 1957
Hpy188I	TCNGA	9 cut(s)	301, 375, 672, 679, 686, 1235, 2107, 2452, 2496
Hpy188III	TCNNGA	7 cut(s)	441, 967, 1000, 1258, 1460, 1532, 2387
Hpy8I	GTNNAC	6 cut(s)	895, 976, 1338, 1591, 1942, 1957
HpyCH4III	ACNGT	6 cut(s)	182, 330, 627, 980, 1870, 2080
HpyCH4IV	ACGT	3 cut(s)	250, 688, 906
HpyCH4V	TGCA	8 cut(s)	545, 1088, 1373, 1661, 1916, 2042, 2422, 2474
HpyF10VI	GCNNNNNNNGC	5 cut(s)	168, 1166, 1181, 1515, 1642
HpySE526I	ACGT	3 cut(s)	250, 688, 906
Hsp92I	GRCGYC	2 cut(s)	68, 688
HspAI	GCGC	1 cut(s)	556
Kpn2I	TCCGGA	1 cut(s)	440
Ksp22I	TGATCA	3 cut(s)	234, 711, 1389
KspAI	GTTAAC	1 cut(s)	1957
LguI	GCTCTTC	1 cut(s)	1635
LmnI	GCTCC	3 cut(s)	519, 1731, 2012
Lsp1109I	GCAGC	2 cut(s)	357, 767
LweI	GCATC	3 cut(s)	709, 1062, 1404
MaeI	CTAG	5 cut(s)	578, 582, 1784, 2022, 2484
MaeII	ACGT	3 cut(s)	250, 688, 906
MaeIII	GTNAC	7 cut(s)	950, 1243, 1667, 1778, 2070, 2149, 2477
MboII	GAAGA	7 cut(s)	257, 582, 1514, 1652, 2140, 2179, 2234
MfeI	CAATTG	3 cut(s)	488, 537, 1032
MflI	RGATCY	1 cut(s)	1886
MhlI	GDGCHC	4 cut(s)	516, 663, 677, 1127
MlyI	GAGTC	2 cut(s)	142, 1156
MmeI	TCCRAC	4 cut(s)	1571, 1909, 2115, 2318
MroI	TCCGGA	1 cut(s)	440
MroXI	GAANNNNTTC	1 cut(s)	589
MseI	TTAA	6 cut(s)	410, 1811, 1859, 1956, 1970, 2513
MslI	CAYNNNNRTG	1 cut(s)	1364
MspI	CCGG	5 cut(s)	441, 612, 635, 1007, 1603
MspR9I	CCNGG	4 cut(s)	782, 1007, 1332, 1604
MunI	CAATTG	3 cut(s)	488, 537, 1032
Mva1269I	GAATGC	2 cut(s)	545, 1120
MvaI	CCWGG	2 cut(s)	782, 1332
MvnI	CGCG	1 cut(s)	1001
MwoI	GCNNNNNNNGC	5 cut(s)	168, 1166, 1181, 1515, 1642
NciI	CCSGG	2 cut(s)	1007, 1604
NcoI	CCATGG	1 cut(s)	564
NlaIV	GGNNCC	2 cut(s)	1198, 1727
NmuCI	GTSAC	5 cut(s)	950, 1667, 1778, 2070, 2149
NruI	TCGCGA	1 cut(s)	1001
NsbI	TGCGCA	1 cut(s)	557
NspI	RCATGY	1 cut(s)	2478
PagI	TCATGA	1 cut(s)	1257
PciSI	GCTCTTC	1 cut(s)	1635
PcsI	WCGNNNNNNNCGW	1 cut(s)	256
PctI	GAATGC	2 cut(s)	545, 1120
PdmI	GAANNNNTTC	1 cut(s)	589
PfeI	GAWTC	6 cut(s)	417, 600, 1327, 1649, 2018, 2083
PflMI	CCANNNNNTGG	1 cut(s)	1306
PfoI	TCCNGGA	1 cut(s)	1005
PkrI	GCNGC	3 cut(s)	344, 347, 757
Ple19I	CGATCG	1 cut(s)	972
PleI	GAGTC	2 cut(s)	142, 1155
PpsI	GAGTC	2 cut(s)	142, 1155
Ppu21I	YACGTR	2 cut(s)	251, 907
PshAI	GACNNNNGTC	1 cut(s)	1947
PshBI	ATTAAT	1 cut(s)	410
PsiI	TTATAA	1 cut(s)	2196
Psp124BI	GAGCTC	3 cut(s)	663, 677, 1127
Psp6I	CCWGG	2 cut(s)	780, 1330
PspGI	CCWGG	2 cut(s)	780, 1330
PspN4I	GGNNCC	2 cut(s)	1198, 1727
PspPI	GGNCC	6 cut(s)	326, 454, 619, 895, 1013, 1426
PstNI	CAGNNNCTG	1 cut(s)	1868
PsuI	RGATCY	1 cut(s)	1886
PvuI	CGATCG	1 cut(s)	972
RruI	TCGCGA	1 cut(s)	1001
RsaI	GTAC	5 cut(s)	494, 797, 1766, 2401, 2458
RsaNI	GTAC	5 cut(s)	493, 796, 1765, 2400, 2457
RseI	CAYNNNNRTG	1 cut(s)	1364
SacI	GAGCTC	3 cut(s)	663, 677, 1127
SalI	GTCGAC	1 cut(s)	1589
SapI	GCTCTTC	1 cut(s)	1635
SaqAI	TTAA	6 cut(s)	410, 1811, 1859, 1956, 1970, 2513
SatI	GCNGC	3 cut(s)	343, 346, 756
Sau96I	GGNCC	6 cut(s)	326, 454, 619, 895, 1013, 1426
SchI	GAGTC	2 cut(s)	142, 1156
ScrFI	CCNGG	4 cut(s)	782, 1007, 1332, 1604
SduI	GDGCHC	4 cut(s)	516, 663, 677, 1127
SfaNI	GCATC	3 cut(s)	709, 1062, 1404
SfcI	CTRYAG	2 cut(s)	166, 1743
SinI	GGWCC	4 cut(s)	326, 454, 895, 1426
SmiMI	CAYNNNNRTG	1 cut(s)	1364
SmlI	CTYRAG	2 cut(s)	269, 1144
SmoI	CTYRAG	2 cut(s)	269, 1144
SsiI	CCGC	3 cut(s)	343, 1019, 2010
SspI	AATATT	1 cut(s)	403
SspMI	CTAG	5 cut(s)	578, 582, 1784, 2022, 2484
SstI	GAGCTC	3 cut(s)	663, 677, 1127
StyD4I	CCNGG	4 cut(s)	780, 1005, 1330, 1602
StyI	CCWWGG	2 cut(s)	38, 564
TaaI	ACNGT	6 cut(s)	182, 330, 627, 980, 1870, 2080
TaiI	ACGT	3 cut(s)	253, 691, 909
TaqI	TCGA	4 cut(s)	151, 701, 914, 1590
TatI	WGTACW	3 cut(s)	492, 2399, 2456
TauI	GCSGC	1 cut(s)	345
TfiI	GAWTC	6 cut(s)	417, 600, 1327, 1649, 2018, 2083
Tru1I	TTAA	6 cut(s)	410, 1811, 1859, 1956, 1970, 2513
Tru9I	TTAA	6 cut(s)	410, 1811, 1859, 1956, 1970, 2513
TscAI	CASTG	3 cut(s)	897, 1171, 2079
TseFI	GTSAC	5 cut(s)	950, 1667, 1778, 2070, 2149
TseI	GCWGC	2 cut(s)	345, 755
Tsp45I	GTSAC	5 cut(s)	950, 1667, 1778, 2070, 2149
TspGWI	ACGGA	2 cut(s)	61, 248
TspRI	CASTG	3 cut(s)	897, 1171, 2079
Van91I	CCANNNNNTGG	1 cut(s)	1306
VpaK11BI	GGWCC	4 cut(s)	326, 454, 895, 1426
VspI	ATTAAT	1 cut(s)	410
XapI	RAATTY	6 cut(s)	11, 353, 379, 386, 585, 2060
XceI	RCATGY	1 cut(s)	2478
XmiI	GTMKAC	1 cut(s)	1590
XmnI	GAANNNNTTC	1 cut(s)	589
XspI	CTAG	5 cut(s)	578, 582, 1784, 2022, 2484
ZraI	GACGTC	1 cut(s)	689

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.