Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010237

Physical Location & Seq

Genomic Coordinates Forward (+)

4494083 .. 4497394

Gene Structure 3312 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1056200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2538 bp

ATGGTTAGGGAATTCCCACTTTACCATCTCCCAACCCCTTGGGATGGAAGTTCAAACAGATTTCAGGCGTCCTTGAAAGCTCAGGCAGACAATGTGTCCATAAATCTCAACAATGTTTACCCTTCAATTATTATTTCATGTGGAAAGGCTCGAGAGGCAGGCGCCTTCAACTTACTTTATGGTGAAAATCCATTGAAAAATTCATTCACACTGTTGTTGTTAGAGTTTGCATTGGTCATCATCCTCACTCGTATCCTCCGTGTTCTTCTCAAGCCTTTCAAACAACCCAGAATTGTCTCAGAAGTCATTGGTGGTATATTCATTGGACCGTCAATTTTAGGCCGCAGTGAAAAATTTACCTCAGTTATGTTCCCCAAAAATTCACAATTCATAGTGAAAAATATTGGAGCAATTGGATTCACTTATTATTTTTTCTTGTCCGGAGTGAAAATGGACATTTCCCTAGTGAAAAAGGCAAATAAGAAACAATTGTACATAGCATTTGCTGGAGTACTCCTCCCTTTCATACTTATATCAATTGTTGCATTCGCTTTGCGCAAGTCCATGGACAAAGAACTAGCTAGAATTTCTTCACTCGGATTCATTTCCTCCGGCTTGGCCCTACCATTGTTTCCGATCATCCACTCCATCCTCAAAGAGCTCAACCTTCTGAGCTCAGAGATCGGACGACTGGCATTGTCGATATCGGTGATAAGCGATGCAATGGGAGTTGGTGTAATGATTGTATTTGAGGCAGCTAAGCAAGGTGAGGGCAACCACCTGGCTGTGTTATGGTACTTGATTTCTTTGGTTGTTTTTCTGGCCTTCATTGTTTTTGTGATTAGGAGGAGTTTGGTGAGAATTGCTGAGACCACCCCAGAGGGGCAGCCAGTGAACCAAGGGTATGTGGTGATGATTTTTTTGGGGGTTTTAGTGATGGGGGGTTTGGCTGACATAACCGGGATCGCGATCGCAAACGGTGCATTCTGGTTAGGTTTGGCGATCCCGGATGGCCCTCCAATTGGATCAACAATTGTGGAGAGGAGTGAGATGGTTATAGTGGATGTTTTGATGCCATTTTCTTTTGCATTGGTTGGGCTTTCTGTGGATGTGAATGCCATGAGCTCTGCTGGTTGGTCAAGCTTGAGTCCCATGTTTGCCATCACTCTGACTGCCTATGTGGCTAAGTTGCTTGGAACCCTAATCACTTCTGCCTTCTTTGAGTTGCCACTCAGGGATGGTGTTACGCTCAGCTTCATGATGATCCTAAAAGGTCAAGTGGAGATAGTGGTTTTTCTCCATTGGATGGACAAAAAGATAATGGGGATTCCAGGGTTTACAATGATGGTGCTATCCCTCACTACGATGACTGCAATATCCACACCCTTGATTAGCATCCTTTACGACCCAACGAGGCCATACATGGTCCATAAACGGAGAACCATCCAGCACACTCCTCAGGCGGACGCAGAACTTCGCATAGTCCTATGTATATATGATGAAGATAGCACAGGTTCCCTCATCAACTTTCTTGAGATTTCCAATCCAACTCTAAGCACCCCGTTCGTAATCTTTTCTCTCCGTCTCGTCGATCTTGTTGGCCGGGCTTCTCCTGTGCTTATAGACCATGAAAAGCAAGAAGAGCATGATTCAAAATATGCAGTTTGTCACACAATCCACAATGTCTTGCAGCAATACCAAGAAACCAAAGGTGAGTGTGTTAGGCTCCATCCCTTCACAGCAATAGTGCCAAATAGAACAATGTACCAAGACATTTGTGACCTAGCTCTTGTGAAAAAGGCCACAATTATAATCTTGCCTTTTCACAAGGAGTGTTTGGATACTGCTGGACGACAATTAACAGGAATTGTGAGGCATGGGGTTGGATCTGTCAATTCAAATGTGTTGGCCCATGCACCTTGCTCAGTTGGAGTTCTGGTTGACAAGGGTCATGTTAGACATAACAAACTGGCAGTTAGAAACACTGAGCTACATTTTGCCGTGCTATTTTTGGGCGGAGCGGATTCTAGGGAGGCATTATGTTATGCAGATAGGATGGCCGGGAACTTAAACGTGTCGCTGACTGTGATTCGGTTTCTTTCGCACAATTTTGTAGGTGATGATGAGATGGAGAAGAAGTTGGATGATGGCGTTGTGACGTGGTTTTGGGTGAAGAATGAGAGGGATGAGAGGGTGAGTTATAGAGAAATAACGGTGAGGAATGGGGAAGAGACAATCTCAGCCATTCAATCAGTGAATGATGATACTGAAAATAATTATGACCTTTGGATTGTGGGAAGGAAACAAGGGATCAATCCTGTGCTTATATCTGGGCTCTCATATTGGAGTGAAAATGATGAGTTGGGAATAATTGGGGACTATATTTCCTCTCTTGATTTTTGTTGTACTGCTTCTGTGTTAGTGGTACAACAGCAGATTTTGAGAGGGCAAGGGTCAGATAGTACAAGTGGAAGGTTTGCATGTAGCCCTAGCCCTACTCAAAAGGTGAAGGATTTGTTGATTTCATGGTGTAGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0006810 GO:0006811 GO:0006812 GO:0006885 GO:0008150 GO:0012505 GO:0015672 GO:0042592 GO:0044464 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055067 GO:0055080 GO:0065007 GO:0065008 GO:0098771

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_H_Exchanger

Protein Analysis

846

Amino Acids

93.29

Weight (kDa)

7.01

Isoelectric Point (pI)

36.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_H_Exchanger	PF00999	80 - 462	4.5e-38	Sodium/hydrogen exchanger, transmembrane
CHX17_2nd	PF23256	523 - 653	1.3e-11	Plant cation/H(+) antiporter domain
CHX17_C	PF23259	665 - 812	2e-09	Plant cation/H(+) antiporter, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010237)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G37060 AT5G58460
fragaria_vesca	FvH4_3g39730
malus_domestica	MD03G1056200.v1.1 MD11G1057100.v1.1 MD11G1057400.v1.1
prunus_persica	Prupe.6G048600_v2.0.a1
pyrus_communis	pycom11g04800 pycom11g04810
rosa_chinensis	RchiOBHm_Chr5g0071611
rosa_laevigata	RLG00000036229
rosa_multiflora	Rmu_sc0005745.1_g000024
rosa_roxburghii	Rroxscaffold_1G00009570
rosa_rugosa	Rorug05G0413600 Rorug05G0413600 Rorug05G0413600
rosa_samantha	Rh5AG469500 Rh5BG488900 Rh5CG512800 Rh5DG499500
rosa_wichuraiana	Rw5G043620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1811
Acc16I	TGCGCA	1 cut(s)	557
AccB1I	GGYRCC	1 cut(s)	161
AccB7I	CCANNNNNTGG	1 cut(s)	1306
AccBSI	CCGCTC	1 cut(s)	2021
AccII	CGCG	1 cut(s)	968
AccIII	TCCGGA	1 cut(s)	440
AciI	CCGC	4 cut(s)	343, 1463, 2016, 2021
AclWI	GGATC	6 cut(s)	971, 997, 1033, 1258, 1894, 2318
AcoI	YGGCCR	2 cut(s)	1600, 2058
AcsI	RAATTY	5 cut(s)	11, 199, 353, 379, 585
AcyI	GRCGYC	2 cut(s)	68, 162
AfaI	GTAC	7 cut(s)	494, 513, 797, 1766, 2407, 2427, 2464
AfiI	CCNNNNNNNGG	4 cut(s)	44, 882, 1022, 1306
AflIII	ACRYGT	1 cut(s)	2073
AgsI	TTSAA	9 cut(s)	54, 76, 126, 169, 196, 280, 1653, 1899, 2249
AhdI	GACNNNNNGTC	1 cut(s)	94
AjiI	CACGTC	1 cut(s)	2160
AjnI	CCWGG	2 cut(s)	780, 1330
Alw21I	GWGCWC	3 cut(s)	663, 677, 1127
Alw26I	GTCTC	4 cut(s)	301, 863, 1589, 2225
AlwI	GGATC	6 cut(s)	971, 997, 1033, 1258, 1894, 2318
Ama87I	CYCGRG	1 cut(s)	150
Aor13HI	TCCGGA	1 cut(s)	440
AoxI	GGCC	9 cut(s)	340, 618, 822, 1012, 1415, 1600, 1800, 1908, 2058
ApeKI	GCWGC	3 cut(s)	755, 886, 1690
ApoI	RAATTY	5 cut(s)	11, 199, 353, 379, 585
AsiSI	GCGATCGC	1 cut(s)	972
Asp700I	GAANNNNTTC	1 cut(s)	589
AspLEI	GCGC	2 cut(s)	164, 558
AspS9I	GGNCC	5 cut(s)	326, 619, 1013, 1426, 1909
AsuC2I	CCSGG	4 cut(s)	961, 1007, 1604, 2062
AvaI	CYCGRG	1 cut(s)	150
AvaII	GGWCC	2 cut(s)	326, 1426
AxyI	CCTNAGG	1 cut(s)	1458
BanI	GGYRCC	1 cut(s)	161
BanII	GRGCYC	4 cut(s)	663, 677, 1127, 2337
Bbv12I	GWGCWC	3 cut(s)	663, 677, 1127
BbvI	GCAGC	3 cut(s)	767, 898, 1702
BceAI	ACGGC	1 cut(s)	1985
BcgI	CGANNNNNNTGC	2 cut(s)	1546, 1580
BciT130I	CCWGG	2 cut(s)	782, 1332
BciVI	GTATCC	2 cut(s)	263, 1834
BcnI	CCSGG	4 cut(s)	961, 1007, 1604, 2062
BcoDI	GTCTC	4 cut(s)	301, 863, 1589, 2225
BfaI	CTAG	6 cut(s)	464, 578, 582, 1784, 2028, 2490
BfoI	RGCGCY	1 cut(s)	165
BfuI	GTATCC	2 cut(s)	263, 1834
BglI	GCCNNNNNGGC	1 cut(s)	1181
BisI	GCNGC	4 cut(s)	343, 756, 887, 1691
BlpI	GCTNAGC	2 cut(s)	759, 1250
BlsI	GCNGC	4 cut(s)	344, 757, 888, 1692
BmcAI	AGTACT	1 cut(s)	513
Bme1390I	CCNGG	6 cut(s)	782, 961, 1007, 1332, 1604, 2062
Bme18I	GGWCC	2 cut(s)	326, 1426
BmeRI	GACNNNNNGTC	1 cut(s)	94
BmeT110I	CYCGRG	1 cut(s)	150
BmgBI	CACGTC	1 cut(s)	2160
BmgT120I	GGNCC	5 cut(s)	326, 619, 1013, 1426, 1909
BmiI	GGNNCC	4 cut(s)	163, 1198, 1516, 1727
BmrFI	CCNGG	6 cut(s)	782, 961, 1007, 1332, 1604, 2062
BmsI	GCATC	3 cut(s)	709, 1062, 1404
BoxI	GACNNNNGTC	1 cut(s)	1947
BplI	GAGNNNNNCTC	4 cut(s)	501, 533, 2222, 2254
BpmI	CTGGAG	1 cut(s)	528
Bpu10I	CCTNAGC	1 cut(s)	81
Bpu1102I	GCTNAGC	2 cut(s)	759, 1250
BpuEI	CTTGAG	3 cut(s)	254, 1165, 1553
BpuMI	CCSGG	4 cut(s)	961, 1007, 1604, 2062
BsaBI	GATNNNNATC	2 cut(s)	968, 1394
BsaHI	GRCGYC	2 cut(s)	68, 162
BsaI	GGTCTC	1 cut(s)	863
BsaJI	CCNNGG	4 cut(s)	38, 564, 898, 1331
BsaWI	WCCGGW	1 cut(s)	440
BsaXI	ACNNNNNCTCC	6 cut(s)	720, 750, 1923, 1953, 2123, 2153
Bsc4I	CCNNNNNNNGG	4 cut(s)	44, 882, 1022, 1306
Bse1I	ACTGG	3 cut(s)	696, 890, 1974
Bse21I	CCTNAGG	1 cut(s)	1458
Bse3DI	GCAATG	1 cut(s)	729
Bse8I	GATNNNNATC	2 cut(s)	968, 1394
BseAI	TCCGGA	1 cut(s)	440
BseBI	CCWGG	2 cut(s)	782, 1332
BseDI	CCNNGG	4 cut(s)	38, 564, 898, 1331
BseJI	GATNNNNATC	2 cut(s)	968, 1394
BseLI	CCNNNNNNNGG	4 cut(s)	44, 882, 1022, 1306
BseMI	GCAATG	1 cut(s)	729
BseNI	ACTGG	3 cut(s)	696, 890, 1974
BseRI	GAGGAG	4 cut(s)	506, 862, 1057, 1446
BseXI	GCAGC	3 cut(s)	767, 898, 1702
Bsh1236I	CGCG	1 cut(s)	968
Bsh1285I	CGRYCG	1 cut(s)	972
BshFI	GGCC	9 cut(s)	342, 620, 824, 1014, 1417, 1602, 1802, 1910, 2060
BshNI	GGYRCC	1 cut(s)	161
BsiEI	CGRYCG	1 cut(s)	972
BsiHKAI	GWGCWC	3 cut(s)	663, 677, 1127
BsiHKCI	CYCGRG	1 cut(s)	150
BsiSI	CCGG	6 cut(s)	441, 612, 960, 1007, 1603, 2061
BslFI	GGGAC	2 cut(s)	1134, 2390
BslI	CCNNNNNNNGG	4 cut(s)	44, 882, 1022, 1306
BsmAI	GTCTC	4 cut(s)	301, 863, 1589, 2225
BsmBI	CGTCTC	1 cut(s)	1589
BsmFI	GGGAC	2 cut(s)	1134, 2390
BsmI	GAATGC	3 cut(s)	545, 983, 1120
BsnI	GGCC	9 cut(s)	342, 620, 824, 1014, 1417, 1602, 1802, 1910, 2060
Bso31I	GGTCTC	1 cut(s)	863
BsoBI	CYCGRG	1 cut(s)	150
Bsp1286I	GDGCHC	4 cut(s)	663, 677, 1127, 2337
Bsp13I	TCCGGA	1 cut(s)	440
Bsp1407I	TGTACA	1 cut(s)	492
Bsp1720I	GCTNAGC	2 cut(s)	759, 1250
Bsp19I	CCATGG	1 cut(s)	564
Bsp68I	TCGCGA	1 cut(s)	968
BspACI	CCGC	4 cut(s)	343, 1463, 2016, 2021
BspANI	GGCC	9 cut(s)	342, 620, 824, 1014, 1417, 1602, 1802, 1910, 2060
BspEI	TCCGGA	1 cut(s)	440
BspFNI	CGCG	1 cut(s)	968
BspHI	TCATGA	1 cut(s)	1257
BspLI	GGNNCC	4 cut(s)	163, 1198, 1516, 1727
BspPI	GGATC	6 cut(s)	971, 997, 1033, 1258, 1894, 2318
BspQI	GCTCTTC	1 cut(s)	1635
BspT107I	GGYRCC	1 cut(s)	161
BspTNI	GGTCTC	1 cut(s)	863
BsrBI	CCGCTC	1 cut(s)	2021
BsrDI	GCAATG	1 cut(s)	729
BsrGI	TGTACA	1 cut(s)	492
BsrI	ACTGG	3 cut(s)	696, 890, 1974
BssECI	CCNNGG	4 cut(s)	38, 564, 898, 1331
BssNI	GRCGYC	2 cut(s)	68, 162
BssT1I	CCWWGG	3 cut(s)	38, 564, 898
Bst2UI	CCWGG	2 cut(s)	782, 1332
Bst4CI	ACNGT	5 cut(s)	213, 330, 980, 2086, 2215
Bst6I	CTCTTC	2 cut(s)	1635, 2223
BstACI	GRCGYC	2 cut(s)	68, 162
BstAPI	GCANNNNNTGC	1 cut(s)	980
BstAUI	TGTACA	1 cut(s)	492
BstC8I	GCNNGC	1 cut(s)	160
BstDSI	CCRYGG	1 cut(s)	564
BstFNI	CGCG	1 cut(s)	968
BstH2I	RGCGCY	1 cut(s)	165
BstHHI	GCGC	2 cut(s)	164, 558
BstMAI	GTCTC	4 cut(s)	301, 863, 1589, 2225
BstMCI	CGRYCG	1 cut(s)	972
BstMWI	GCNNNNNNNGC	4 cut(s)	155, 980, 1181, 1642
BstNI	CCWGG	2 cut(s)	782, 1332
BstNSI	RCATGY	1 cut(s)	2484
BstPAI	GACNNNNGTC	1 cut(s)	1947
BstSCI	CCNGG	6 cut(s)	780, 959, 1005, 1330, 1602, 2060
BstUI	CGCG	1 cut(s)	968
BstV1I	GCAGC	3 cut(s)	767, 898, 1702
BstX2I	RGATCY	1 cut(s)	1886
BstXI	CCANNNNNNTGG	1 cut(s)	39
BstYI	RGATCY	1 cut(s)	1886
Bsu36I	CCTNAGG	1 cut(s)	1458
BsuI	GTATCC	2 cut(s)	263, 1834
BsuRI	GGCC	9 cut(s)	342, 620, 824, 1014, 1417, 1602, 1802, 1910, 2060
BtgI	CCRYGG	1 cut(s)	564
BtgZI	GCGATG	1 cut(s)	732
BtrI	CACGTC	1 cut(s)	2160
BtsI	GCAGTG	1 cut(s)	352
BtsIMutI	CAGTG	5 cut(s)	209, 352, 897, 1983, 2259
BtuMI	TCGCGA	1 cut(s)	968
Cac8I	GCNNGC	1 cut(s)	160
CciI	TCATGA	1 cut(s)	1257
CfoI	GCGC	2 cut(s)	164, 558
Cfr13I	GGNCC	5 cut(s)	326, 619, 1013, 1426, 1909
CseI	GACGC	2 cut(s)	57, 1475
Csp6I	GTAC	7 cut(s)	493, 512, 796, 1765, 2406, 2426, 2463
CspCI	CAANNNNNGTGG	2 cut(s)	1017, 1052
CviQI	GTAC	7 cut(s)	493, 512, 796, 1765, 2406, 2426, 2463
DinI	GGCGCC	1 cut(s)	163
DriI	GACNNNNNGTC	1 cut(s)	94
EaeI	YGGCCR	2 cut(s)	1600, 2058
Eam1104I	CTCTTC	2 cut(s)	1635, 2223
Eam1105I	GACNNNNNGTC	1 cut(s)	94
EarI	CTCTTC	2 cut(s)	1635, 2223
EciI	GGCGGA	2 cut(s)	1478, 2031
Ecl136II	GAGCTC	3 cut(s)	661, 675, 1125
Eco130I	CCWWGG	3 cut(s)	38, 564, 898
Eco24I	GRGCYC	4 cut(s)	663, 677, 1127, 2337
Eco31I	GGTCTC	1 cut(s)	863
Eco32I	GATATC	1 cut(s)	705
Eco47I	GGWCC	2 cut(s)	326, 1426
Eco53kI	GAGCTC	3 cut(s)	661, 675, 1125
Eco81I	CCTNAGG	1 cut(s)	1458
Eco88I	CYCGRG	1 cut(s)	150
EcoICRI	GAGCTC	3 cut(s)	661, 675, 1125
EcoRI	GAATTC	1 cut(s)	11
EcoRII	CCWGG	2 cut(s)	780, 1330
EcoRV	GATATC	1 cut(s)	705
EcoT14I	CCWWGG	3 cut(s)	38, 564, 898
EcoT38I	GRGCYC	4 cut(s)	663, 677, 1127, 2337
EgeI	GGCGCC	1 cut(s)	163
EheI	GGCGCC	1 cut(s)	163
ErhI	CCWWGG	3 cut(s)	38, 564, 898
Esp3I	CGTCTC	1 cut(s)	1589
FaqI	GGGAC	2 cut(s)	1134, 2390
Fnu4HI	GCNGC	4 cut(s)	343, 756, 887, 1691
FriOI	GRGCYC	4 cut(s)	663, 677, 1127, 2337
Fsp4HI	GCNGC	4 cut(s)	343, 756, 887, 1691
FspBI	CTAG	6 cut(s)	464, 578, 582, 1784, 2028, 2490
FspI	TGCGCA	1 cut(s)	557
GlaI	GCGC	2 cut(s)	163, 557
GluI	GCNGC	4 cut(s)	343, 756, 887, 1691
GsuI	CTGGAG	1 cut(s)	528
HaeII	RGCGCY	1 cut(s)	165
HaeIII	GGCC	9 cut(s)	342, 620, 824, 1014, 1417, 1602, 1802, 1910, 2060
HapII	CCGG	6 cut(s)	441, 612, 960, 1007, 1603, 2061
HgaI	GACGC	2 cut(s)	57, 1475
HhaI	GCGC	2 cut(s)	164, 558
Hin1I	GRCGYC	2 cut(s)	68, 162
Hin6I	GCGC	2 cut(s)	162, 556
HinP1I	GCGC	2 cut(s)	162, 556
HincII	GTYRAC	1 cut(s)	1942
HindII	GTYRAC	1 cut(s)	1942
HindIII	AAGCTT	1 cut(s)	1141
HinfI	GANTC	7 cut(s)	417, 600, 1147, 1327, 1649, 2024, 2089
HpaII	CCGG	6 cut(s)	441, 612, 960, 1007, 1603, 2061
Hpy166II	GTNNAC	4 cut(s)	118, 895, 1338, 1942
Hpy188I	TCNGA	8 cut(s)	301, 599, 636, 672, 679, 686, 1170, 2458
Hpy188III	TCNNGA	6 cut(s)	152, 441, 967, 1258, 1532, 2393
Hpy8I	GTNNAC	4 cut(s)	118, 895, 1338, 1942
Hpy99I	CGWCG	1 cut(s)	1592
HpyAV	CCTTC	9 cut(s)	132, 175, 677, 835, 1225, 1744, 2292, 2466, 2503
HpyCH4III	ACNGT	5 cut(s)	213, 330, 980, 2086, 2215
HpyCH4IV	ACGT	2 cut(s)	2073, 2159
HpyF10VI	GCNNNNNNNGC	4 cut(s)	155, 980, 1181, 1642
HpySE526I	ACGT	2 cut(s)	2073, 2159
Hsp92I	GRCGYC	2 cut(s)	68, 162
HspAI	GCGC	2 cut(s)	162, 556
KasI	GGCGCC	1 cut(s)	161
Kpn2I	TCCGGA	1 cut(s)	440
LguI	GCTCTTC	1 cut(s)	1635
LmnI	GCTCC	3 cut(s)	407, 1731, 2018
Lsp1109I	GCAGC	3 cut(s)	767, 898, 1702
LweI	GCATC	3 cut(s)	709, 1062, 1404
MaeI	CTAG	6 cut(s)	464, 578, 582, 1784, 2028, 2490
MaeII	ACGT	2 cut(s)	2073, 2159
MaeIII	GTNAC	4 cut(s)	1243, 1667, 1778, 2155
MbiI	CCGCTC	1 cut(s)	2021
MboII	GAAGA	7 cut(s)	257, 582, 1514, 1652, 2146, 2185, 2240
MfeI	CAATTG	5 cut(s)	411, 488, 537, 1020, 1032
MflI	RGATCY	1 cut(s)	1886
MhlI	GDGCHC	4 cut(s)	663, 677, 1127, 2337
Mly113I	GGCGCC	1 cut(s)	162
MlyI	GAGTC	1 cut(s)	1156
MmeI	TCCRAC	4 cut(s)	1571, 1864, 1909, 2121
MroI	TCCGGA	1 cut(s)	440
MroXI	GAANNNNTTC	1 cut(s)	589
MseI	TTAA	2 cut(s)	1859, 2069
MslI	CAYNNNNRTG	1 cut(s)	1364
MspI	CCGG	6 cut(s)	441, 612, 960, 1007, 1603, 2061
MspR9I	CCNGG	6 cut(s)	782, 961, 1007, 1332, 1604, 2062
MunI	CAATTG	5 cut(s)	411, 488, 537, 1020, 1032
Mva1269I	GAATGC	3 cut(s)	545, 983, 1120
MvaI	CCWGG	2 cut(s)	782, 1332
MvnI	CGCG	1 cut(s)	968
MwoI	GCNNNNNNNGC	4 cut(s)	155, 980, 1181, 1642
NarI	GGCGCC	1 cut(s)	162
NciI	CCSGG	4 cut(s)	961, 1007, 1604, 2062
NcoI	CCATGG	1 cut(s)	564
NlaIV	GGNNCC	4 cut(s)	163, 1198, 1516, 1727
NmuCI	GTSAC	3 cut(s)	1667, 1778, 2155
NruI	TCGCGA	1 cut(s)	968
NsbI	TGCGCA	1 cut(s)	557
NspI	RCATGY	1 cut(s)	2484
PaeR7I	CTCGAG	1 cut(s)	150
PagI	TCATGA	1 cut(s)	1257
PciSI	GCTCTTC	1 cut(s)	1635
PcsI	WCGNNNNNNNCGW	1 cut(s)	256
PctI	GAATGC	3 cut(s)	545, 983, 1120
PdmI	GAANNNNTTC	1 cut(s)	589
PfeI	GAWTC	6 cut(s)	417, 600, 1327, 1649, 2024, 2089
PflMI	CCANNNNNTGG	1 cut(s)	1306
PfoI	TCCNGGA	1 cut(s)	1005
PkrI	GCNGC	4 cut(s)	344, 757, 888, 1692
Ple19I	CGATCG	1 cut(s)	972
PleI	GAGTC	1 cut(s)	1155
PluTI	GGCGCC	1 cut(s)	165
PpsI	GAGTC	1 cut(s)	1155
PshAI	GACNNNNGTC	1 cut(s)	1947
PsiI	TTATAA	1 cut(s)	1811
Psp124BI	GAGCTC	3 cut(s)	663, 677, 1127
Psp6I	CCWGG	2 cut(s)	780, 1330
PspGI	CCWGG	2 cut(s)	780, 1330
PspN4I	GGNNCC	4 cut(s)	163, 1198, 1516, 1727
PspPI	GGNCC	5 cut(s)	326, 619, 1013, 1426, 1909
PsuI	RGATCY	1 cut(s)	1886
PvuI	CGATCG	1 cut(s)	972
RgaI	GCGATCGC	1 cut(s)	972
RruI	TCGCGA	1 cut(s)	968
RsaI	GTAC	7 cut(s)	494, 513, 797, 1766, 2407, 2427, 2464
RsaNI	GTAC	7 cut(s)	493, 512, 796, 1765, 2406, 2426, 2463
RseI	CAYNNNNRTG	1 cut(s)	1364
SacI	GAGCTC	3 cut(s)	663, 677, 1127
SapI	GCTCTTC	1 cut(s)	1635
SaqAI	TTAA	2 cut(s)	1859, 2069
SatI	GCNGC	4 cut(s)	343, 756, 887, 1691
Sau96I	GGNCC	5 cut(s)	326, 619, 1013, 1426, 1909
ScaI	AGTACT	1 cut(s)	513
SchI	GAGTC	1 cut(s)	1156
ScrFI	CCNGG	6 cut(s)	782, 961, 1007, 1332, 1604, 2062
SduI	GDGCHC	4 cut(s)	663, 677, 1127, 2337
SfaAI	GCGATCGC	1 cut(s)	972
SfaNI	GCATC	3 cut(s)	709, 1062, 1404
SfoI	GGCGCC	1 cut(s)	163
Sfr274I	CTCGAG	1 cut(s)	150
SgfI	GCGATCGC	1 cut(s)	972
SinI	GGWCC	2 cut(s)	326, 1426
SlaI	CTCGAG	1 cut(s)	150
SmiMI	CAYNNNNRTG	1 cut(s)	1364
SmlI	CTYRAG	4 cut(s)	150, 269, 1144, 1532
SmoI	CTYRAG	4 cut(s)	150, 269, 1144, 1532
SsiI	CCGC	4 cut(s)	343, 1463, 2016, 2021
SspDI	GGCGCC	1 cut(s)	161
SspI	AATATT	1 cut(s)	403
SspMI	CTAG	6 cut(s)	464, 578, 582, 1784, 2028, 2490
SstI	GAGCTC	3 cut(s)	663, 677, 1127
StyD4I	CCNGG	6 cut(s)	780, 959, 1005, 1330, 1602, 2060
StyI	CCWWGG	3 cut(s)	38, 564, 898
TaaI	ACNGT	5 cut(s)	213, 330, 980, 2086, 2215
TaiI	ACGT	2 cut(s)	2076, 2162
TaqI	TCGA	3 cut(s)	151, 701, 1590
TatI	WGTACW	4 cut(s)	492, 511, 2405, 2462
TauI	GCSGC	1 cut(s)	345
TfiI	GAWTC	6 cut(s)	417, 600, 1327, 1649, 2024, 2089
Tru1I	TTAA	2 cut(s)	1859, 2069
Tru9I	TTAA	2 cut(s)	1859, 2069
TscAI	CASTG	5 cut(s)	216, 352, 897, 1990, 2259
TseFI	GTSAC	3 cut(s)	1667, 1778, 2155
TseI	GCWGC	3 cut(s)	755, 886, 1690
Tsp45I	GTSAC	3 cut(s)	1667, 1778, 2155
TspGWI	ACGGA	3 cut(s)	248, 1450, 1571
TspRI	CASTG	5 cut(s)	216, 352, 897, 1990, 2259
Van91I	CCANNNNNTGG	1 cut(s)	1306
VpaK11BI	GGWCC	2 cut(s)	326, 1426
XapI	RAATTY	5 cut(s)	11, 199, 353, 379, 585
XceI	RCATGY	1 cut(s)	2484
XhoI	CTCGAG	1 cut(s)	150
XmnI	GAANNNNTTC	1 cut(s)	589
XspI	CTAG	6 cut(s)	464, 578, 582, 1784, 2028, 2490
ZrmI	AGTACT	1 cut(s)	513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.