AT5G58690

phospholipase c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007017
Physical Location & Seq
Reverse (-)
23709529 .. 23712558
3030 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58690.3

Sequence Viewer

Length: 1737 bp
ATGAAGAGAGATATGGGGAGTTACAAAATGGGTTTGTGTTGCAGTGACAAGCTCAGGATGAACAGAGGAGCTCCACCTCAGGATGTGGTGACGGCGTTTGTAGAGTATACCGAGGGCAGGAGCCACATGACGGCAGAGCAGCTATGTCGGTTCTTGGTTGAGGTGCAGGACGAGACTGAAGTGTTGGTTTCAGATGCAGAGAAGATCATAGAAAGAATCACATGTGAAAGACATCATATCACTAAGTTCTTGAGGCATACGCTGAACCTCGATGATTTCTTCAGTTTCTTGTTCTCAGATGATCTGAATCATCCAATTGATAGTAAGGTGCATCAAGACATGGCCTCTCCCTTGTCGCACTATTTCATTTACACCAGCCATAATTCGTATCTGACTGGAAATCAGATCAACAGCGAATGCAGCGACGTACCTCTCATAAAGGCATTAAAGAGAGGTGTTCGAGCTCTTGAACTTGACATGTGGCCTAACTCTACTAAGGACGACATACTTGTTCTCCATGGCTGGGCATGGACACCTCCAGTTGAACTAGTCAAATGCTTGAGATCTATAAAAGAACATGCCTTTTATGCATCTGCATACCCTGTGATCTTAACTTTGGAAGATCATTTGACTCCAGATCTTCAAGCCAAAGCAGCTGAGATGATGAAAGAGATATTCATGGATATGGTGTATTTTCCAGAGGCTGGTGGTTTAAAAGAGTTTCCTTCACCAGAAGATCTCAAGTACAAAATTGTAATATCTACAAAGCCTCCTAAAGGTAGCTTAAGAAAGGACAAGGATTCTGAGTCAGATGCATCAGGAAAGGCGTCATCAGATGTTTCAGCAGACGATGAGAAGACTGAGGAAGAAACAAGTGAGGCCAAAAATGAAGAGGATGGATTTGATCAAGAATCCAGCAACTTGGATTTCCTCACCTATAGTCGCCTAATAACGATTCCTTCTGGAAATGCTAAAAATGGATTAAAGGAAGCACTAACAATCGATAATGGTGGAGTTAGACGGCTGAGTTTGAGGGAGCAGAAGTTCAAGAAAGCCACTGAAATGTATGGAACCGAAGTCATAAAATTCACACAGAAGAATCTGTTGAGAATATATCCCAAGGCTACCCGAGTAAATTCCTCCAACTATAGGCCATACAACGGTTGGATGTACGGAGCTCAAATGGTCGCTTTCAACATGCAGGGCTATGGAAGAGCTCTTTGGATGATGCATGGTATGTTTAGGGGAAATGGTGGATGCGGGTACGTGAAAAAACCGGACTTTATGATGAACAATAATCTCAGTGGAGAAGTGTTCAACCCGAAAGCGAAGTTACCCATAAAGAAAACTCTAAAAGTGAAAGTATACATGGGCAAAGGATGGGACTCTGGTTTCCAGCGAACATGTTTCAACACTTGGTCCTCTCCTAACTTCTACACAAGGGTGGGGATAACAGGAGTAAGAGGTGATAAGGTAATGAAGAAAACAAAGAAAGAACAAAAGACATGGGAGCCATTCTGGAACGAGGAGTTTGAGTTTCAGCTAACAGTACCGGAGCTGGCGTTGCTAAGAATAGAAGTTCACGACTACAACATGCCAGAGAAGGACGATTTCTCGGGTCAGACATGTTTGCCTGTGTCAGAGCTGAGACAAGGCATACGTTCTGTTCCTTTGTATGATCGCAAAGGCGAGAGACTAGTTTCAGTTACACTTCTCATGCGGTTTCACTTTCTTTAA

Protein Analysis

578

Amino Acids

66.25

Weight (kDa)

6.93

Isoelectric Point (pI)

41.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_like PF09279 27 - 102 6.2e-07 Phosphoinositide-specific phospholipase C, efhand-like
PI-PLC-X PF00388 114 - 256 2.3e-52 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 342 - 428 1.2e-27 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 450 - 552 5.2e-15 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 704
AccI GTMKAC 2 cut(s) 107, 1365
AciI CCGC 2 cut(s) 1260, 1720
AcsI RAATTY 2 cut(s) 1085, 1135
AcuI CTGAAG 2 cut(s) 198, 265
AcyI GRCGYC 1 cut(s) 827
AfaI GTAC 5 cut(s) 429, 746, 1172, 1265, 1551
AfiI CCNNNNNNNGG 7 cut(s) 117, 130, 523, 704, 776, 1149, 1160
AflII CTTAAG 1 cut(s) 784
AflIII ACRYGT 4 cut(s) 221, 477, 1403, 1625
AgsI TTSAA 7 cut(s) 470, 545, 644, 1048, 1195, 1318, 1411
AhlI ACTAGT 2 cut(s) 547, 1696
AjuI GAANNNNNNNTTGG 2 cut(s) 1204, 1236
AleI CACNNNNGTG 1 cut(s) 1442
AloI GAACNNNNNNTCC 2 cut(s) 1028, 1060
Alw21I GWGCWC 4 cut(s) 73, 466, 1180, 1219
Alw26I GTCTC 3 cut(s) 167, 1642, 1687
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 2 cut(s) 1128, 1615
AoxI GGCC 4 cut(s) 342, 482, 879, 1151
ApeKI GCWGC 3 cut(s) 139, 420, 653
ApoI RAATTY 2 cut(s) 1085, 1135
AspS9I GGNCC 1 cut(s) 1419
AsuHPI GGTGA 4 cut(s) 100, 720, 925, 1478
AvaI CYCGRG 2 cut(s) 1128, 1615
AvaII GGWCC 1 cut(s) 1419
AxyI CCTNAGG 1 cut(s) 78
BanII GRGCYC 4 cut(s) 73, 466, 1180, 1219
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 4 cut(s) 73, 466, 1180, 1219
BbvI GCAGC 3 cut(s) 151, 432, 665
BccI CCATC 2 cut(s) 890, 1374
BceAI ACGGC 3 cut(s) 108, 147, 1037
BcgI CGANNNNNNTGC 2 cut(s) 128, 162
BclI TGATCA 1 cut(s) 904
BcoDI GTCTC 3 cut(s) 167, 1642, 1687
BcuI ACTAGT 2 cut(s) 547, 1696
BfaI CTAG 2 cut(s) 548, 1697
BfmI CTRYAG 2 cut(s) 937, 1147
BfrI CTTAAG 1 cut(s) 784
BglII AGATCT 3 cut(s) 563, 637, 736
BisI GCNGC 3 cut(s) 140, 421, 654
BlsI GCNGC 3 cut(s) 141, 422, 655
Bme18I GGWCC 1 cut(s) 1419
BmeT110I CYCGRG 2 cut(s) 1128, 1615
BmgT120I GGNCC 1 cut(s) 1419
BmiI GGNNCC 3 cut(s) 122, 1072, 1512
BmsI GCATC 7 cut(s) 184, 340, 599, 802, 824, 1218, 1247
BpiI GAAGAC 1 cut(s) 863
BpmI CTGGAG 2 cut(s) 522, 618
Bpu10I CCTNAGC 1 cut(s) 53
BpuEI CTTGAG 3 cut(s) 271, 580, 725
Bsa29I ATCGAT 1 cut(s) 1002
BsaAI YACGTR 1 cut(s) 1267
BsaBI GATNNNNATC 1 cut(s) 306
BsaHI GRCGYC 1 cut(s) 827
BsaJI CCNNGG 3 cut(s) 111, 517, 1119
BsaWI WCCGGW 2 cut(s) 1276, 1552
Bsc4I CCNNNNNNNGG 7 cut(s) 117, 130, 523, 704, 776, 1149, 1160
Bse1I ACTGG 2 cut(s) 400, 539
Bse21I CCTNAGG 1 cut(s) 78
Bse8I GATNNNNATC 1 cut(s) 306
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 3 cut(s) 111, 517, 1119
BseGI GGATG 8 cut(s) 63, 88, 310, 901, 1173, 1230, 1262, 1385
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 7 cut(s) 117, 130, 523, 704, 776, 1149, 1160
BseMII CTCAG 9 cut(s) 67, 92, 309, 648, 795, 852, 1016, 1315, 1637
BseNI ACTGG 2 cut(s) 400, 539
BseRI GAGGAG 2 cut(s) 81, 1541
BseXI GCAGC 3 cut(s) 151, 432, 665
BseYI CCCAGC 1 cut(s) 522
BsgI GTGCAG 1 cut(s) 185
BshFI GGCC 4 cut(s) 344, 484, 881, 1153
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 4 cut(s) 73, 466, 1180, 1219
BsiHKCI CYCGRG 2 cut(s) 1128, 1615
BsiSI CCGG 2 cut(s) 1277, 1553
BslFI GGGAC 1 cut(s) 1397
BslI CCNNNNNNNGG 7 cut(s) 117, 130, 523, 704, 776, 1149, 1160
BsmAI GTCTC 3 cut(s) 167, 1642, 1687
BsmFI GGGAC 1 cut(s) 1397
BsmI GAATGC 1 cut(s) 422
BsnI GGCC 4 cut(s) 344, 484, 881, 1153
BsoBI CYCGRG 2 cut(s) 1128, 1615
Bsp1286I GDGCHC 4 cut(s) 73, 466, 1180, 1219
Bsp19I CCATGG 1 cut(s) 517
BspACI CCGC 2 cut(s) 1260, 1720
BspANI GGCC 4 cut(s) 344, 484, 881, 1153
BspCNI CTCAG 9 cut(s) 66, 91, 308, 649, 796, 853, 1017, 1314, 1638
BspDI ATCGAT 1 cut(s) 1002
BspLI GGNNCC 3 cut(s) 122, 1072, 1512
BspQI GCTCTTC 1 cut(s) 1207
BspTI CTTAAG 1 cut(s) 784
BsrI ACTGG 2 cut(s) 400, 539
BssECI CCNNGG 3 cut(s) 111, 517, 1119
BssNAI GTATAC 2 cut(s) 108, 1366
BssNI GRCGYC 1 cut(s) 827
BssT1I CCWWGG 2 cut(s) 517, 1119
Bst1107I GTATAC 2 cut(s) 108, 1366
Bst4CI ACNGT 2 cut(s) 1163, 1549
Bst6I CTCTTC 2 cut(s) 885, 1207
BstACI GRCGYC 1 cut(s) 827
BstAFI CTTAAG 1 cut(s) 784
BstBAI YACGTR 1 cut(s) 1267
BstC8I GCNNGC 1 cut(s) 1560
BstDSI CCRYGG 1 cut(s) 517
BstENI CCTNNNNNAGG 1 cut(s) 774
BstF5I GGATG 8 cut(s) 63, 88, 310, 901, 1173, 1230, 1262, 1385
BstMAI GTCTC 3 cut(s) 167, 1642, 1687
BstMWI GCNNNNNNNGC 4 cut(s) 420, 587, 653, 1564
BstNSI RCATGY 7 cut(s) 225, 481, 581, 1201, 1407, 1597, 1629
BstSFI CTRYAG 2 cut(s) 937, 1147
BstV1I GCAGC 3 cut(s) 151, 432, 665
BstV2I GAAGAC 1 cut(s) 863
BstX2I RGATCY 3 cut(s) 563, 637, 736
BstXI CCANNNNNNTGG 1 cut(s) 922
BstYI RGATCY 3 cut(s) 563, 637, 736
BstZ17I GTATAC 2 cut(s) 108, 1366
Bsu15I ATCGAT 1 cut(s) 1002
Bsu36I CCTNAGG 1 cut(s) 78
BsuRI GGCC 4 cut(s) 344, 484, 881, 1153
BsuTUI ATCGAT 1 cut(s) 1002
BtgI CCRYGG 1 cut(s) 517
BtsCI GGATG 8 cut(s) 63, 88, 310, 901, 1173, 1230, 1262, 1385
BtsI GCAGTG 1 cut(s) 49
BtsIMutI CAGTG 3 cut(s) 49, 1056, 1309
Cac8I GCNNGC 1 cut(s) 1560
CaiI CAGNNNCTG 1 cut(s) 704
Cfr13I GGNCC 1 cut(s) 1419
ClaI ATCGAT 1 cut(s) 1002
CseI GACGC 1 cut(s) 816
Csp6I GTAC 5 cut(s) 428, 745, 1171, 1264, 1550
CviQI GTAC 5 cut(s) 428, 745, 1171, 1264, 1550
DraI TTTAAA 1 cut(s) 714
Eam1104I CTCTTC 2 cut(s) 885, 1207
EarI CTCTTC 2 cut(s) 885, 1207
Ecl136II GAGCTC 4 cut(s) 71, 464, 1178, 1217
Eco130I CCWWGG 2 cut(s) 517, 1119
Eco24I GRGCYC 4 cut(s) 73, 466, 1180, 1219
Eco47I GGWCC 1 cut(s) 1419
Eco53kI GAGCTC 4 cut(s) 71, 464, 1178, 1217
Eco57I CTGAAG 2 cut(s) 198, 265
Eco81I CCTNAGG 1 cut(s) 78
Eco88I CYCGRG 2 cut(s) 1128, 1615
EcoICRI GAGCTC 4 cut(s) 71, 464, 1178, 1217
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoT14I CCWWGG 2 cut(s) 517, 1119
EcoT22I ATGCAT 3 cut(s) 592, 817, 1233
EcoT38I GRGCYC 4 cut(s) 73, 466, 1180, 1219
ErhI CCWWGG 2 cut(s) 517, 1119
FaqI GGGAC 1 cut(s) 1397
FauI CCCGC 1 cut(s) 1253
FbaI TGATCA 1 cut(s) 904
FblI GTMKAC 2 cut(s) 107, 1365
Fnu4HI GCNGC 3 cut(s) 140, 421, 654
FokI GGATG 8 cut(s) 70, 95, 297, 908, 1180, 1237, 1269, 1392
FriOI GRGCYC 4 cut(s) 73, 466, 1180, 1219
Fsp4HI GCNGC 3 cut(s) 140, 421, 654
FspBI CTAG 2 cut(s) 548, 1697
GluI GCNGC 3 cut(s) 140, 421, 654
GsaI CCCAGC 1 cut(s) 526
GsuI CTGGAG 2 cut(s) 522, 618
HaeIII GGCC 4 cut(s) 344, 484, 881, 1153
HapII CCGG 2 cut(s) 1277, 1553
HgaI GACGC 1 cut(s) 816
Hin1I GRCGYC 1 cut(s) 827
HinfI GANTC 9 cut(s) 216, 307, 631, 800, 806, 911, 955, 1099, 1385
HpaII CCGG 2 cut(s) 1277, 1553
HphI GGTGA 4 cut(s) 100, 720, 925, 1478
Hpy166II GTNNAC 3 cut(s) 108, 1366, 1582
Hpy8I GTNNAC 3 cut(s) 108, 1366, 1582
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 3 cut(s) 735, 969, 1597
HpyCH4III ACNGT 2 cut(s) 1163, 1549
HpyCH4IV ACGT 3 cut(s) 426, 1266, 1660
HpyF10VI GCNNNNNNNGC 4 cut(s) 420, 587, 653, 1564
HpySE526I ACGT 3 cut(s) 426, 1266, 1660
Hsp92I GRCGYC 1 cut(s) 827
Ksp22I TGATCA 1 cut(s) 904
LguI GCTCTTC 1 cut(s) 1207
LmnI GCTCC 7 cut(s) 68, 76, 120, 1036, 1175, 1510, 1555
Lsp1109I GCAGC 3 cut(s) 151, 432, 665
LweI GCATC 7 cut(s) 184, 340, 599, 802, 824, 1218, 1247
MaeI CTAG 2 cut(s) 548, 1697
MaeII ACGT 3 cut(s) 426, 1266, 1660
MaeIII GTNAC 5 cut(s) 20, 44, 88, 1332, 1705
MfeI CAATTG 1 cut(s) 315
MflI RGATCY 3 cut(s) 563, 637, 736
MhlI GDGCHC 4 cut(s) 73, 466, 1180, 1219
MluCI AATT 5 cut(s) 315, 382, 750, 1085, 1135
MlyI GAGTC 3 cut(s) 625, 815, 1379
MmeI TCCRAC 2 cut(s) 1145, 1167
Mph1103I ATGCAT 3 cut(s) 592, 817, 1233
MseI TTAA 6 cut(s) 446, 611, 713, 785, 983, 1735
MslI CAYNNNNRTG 3 cut(s) 683, 1061, 1442
MspA1I CMGCKG 1 cut(s) 656
MspCI CTTAAG 1 cut(s) 784
MspI CCGG 2 cut(s) 1277, 1553
MunI CAATTG 1 cut(s) 315
Mva1269I GAATGC 1 cut(s) 422
MwoI GCNNNNNNNGC 4 cut(s) 420, 587, 653, 1564
NcoI CCATGG 1 cut(s) 517
NlaIV GGNNCC 3 cut(s) 122, 1072, 1512
NmuCI GTSAC 2 cut(s) 44, 88
NsiI ATGCAT 3 cut(s) 592, 817, 1233
NspI RCATGY 7 cut(s) 225, 481, 581, 1201, 1407, 1597, 1629
OliI CACNNNNGTG 1 cut(s) 1442
PciI ACATGT 4 cut(s) 221, 477, 1403, 1625
PciSI GCTCTTC 1 cut(s) 1207
PctI GAATGC 1 cut(s) 422
PfeI GAWTC 6 cut(s) 216, 307, 800, 911, 955, 1099
PflMI CCANNNNNTGG 1 cut(s) 704
PkrI GCNGC 3 cut(s) 141, 422, 655
PleI GAGTC 3 cut(s) 625, 814, 1379
PpsI GAGTC 3 cut(s) 625, 814, 1379
Ppu21I YACGTR 1 cut(s) 1267
PscI ACATGT 4 cut(s) 221, 477, 1403, 1625
Psp124BI GAGCTC 4 cut(s) 73, 466, 1180, 1219
PspFI CCCAGC 1 cut(s) 522
PspN4I GGNNCC 3 cut(s) 122, 1072, 1512
PspPI GGNCC 1 cut(s) 1419
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 3 cut(s) 563, 637, 736
PvuII CAGCTG 1 cut(s) 656
RsaI GTAC 5 cut(s) 429, 746, 1172, 1265, 1551
RsaNI GTAC 5 cut(s) 428, 745, 1171, 1264, 1550
RseI CAYNNNNRTG 3 cut(s) 683, 1061, 1442
SacI GAGCTC 4 cut(s) 73, 466, 1180, 1219
SapI GCTCTTC 1 cut(s) 1207
SaqAI TTAA 6 cut(s) 446, 611, 713, 785, 983, 1735
SatI GCNGC 3 cut(s) 140, 421, 654
Sau96I GGNCC 1 cut(s) 1419
SchI GAGTC 3 cut(s) 625, 815, 1379
SduI GDGCHC 4 cut(s) 73, 466, 1180, 1219
SfaNI GCATC 7 cut(s) 184, 340, 599, 802, 824, 1218, 1247
SfcI CTRYAG 2 cut(s) 937, 1147
SinI GGWCC 1 cut(s) 1419
SmiMI CAYNNNNRTG 3 cut(s) 683, 1061, 1442
SmlI CTYRAG 4 cut(s) 250, 559, 740, 784
SmoI CTYRAG 4 cut(s) 250, 559, 740, 784
SpeI ACTAGT 2 cut(s) 547, 1696
Sse9I AATT 5 cut(s) 315, 382, 750, 1085, 1135
SsiI CCGC 2 cut(s) 1260, 1720
SspMI CTAG 2 cut(s) 548, 1697
SstI GAGCTC 4 cut(s) 73, 466, 1180, 1219
StyI CCWWGG 2 cut(s) 517, 1119
TaaI ACNGT 2 cut(s) 1163, 1549
TaiI ACGT 3 cut(s) 429, 1269, 1663
TaqI TCGA 3 cut(s) 270, 460, 1002
TasI AATT 5 cut(s) 315, 382, 750, 1085, 1135
TatI WGTACW 1 cut(s) 744
TfiI GAWTC 6 cut(s) 216, 307, 800, 911, 955, 1099
Tru1I TTAA 6 cut(s) 446, 611, 713, 785, 983, 1735
Tru9I TTAA 6 cut(s) 446, 611, 713, 785, 983, 1735
TscAI CASTG 3 cut(s) 49, 1063, 1309
TseFI GTSAC 2 cut(s) 44, 88
TseI GCWGC 3 cut(s) 139, 420, 653
Tsp45I GTSAC 2 cut(s) 44, 88
TspDTI ATGAA 8 cut(s) 17, 74, 355, 667, 680, 903, 1304, 1493
TspGWI ACGGA 1 cut(s) 1188
TspRI CASTG 3 cut(s) 49, 1063, 1309
Van91I CCANNNNNTGG 1 cut(s) 704
Vha464I CTTAAG 1 cut(s) 784
VpaK11BI GGWCC 1 cut(s) 1419
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 2 cut(s) 1085, 1135
XceI RCATGY 7 cut(s) 225, 481, 581, 1201, 1407, 1597, 1629
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XmiI GTMKAC 2 cut(s) 107, 1365
XspI CTAG 2 cut(s) 548, 1697
Zsp2I ATGCAT 3 cut(s) 592, 817, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.