Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007017

Physical Location & Seq

Genomic Coordinates Reverse (-)

5605718 .. 5609597

Gene Structure 3880 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1069000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGGGAGTTATAGGATGTGCGTGTGCTTCACTAGGAAGTTCAGGGTGAAGGAGGAAGAGCCACCGCGGGACGTGAAGGAAGCATTCGAAAAGTACGCTGAGGGTGGGACCCAAATGACAGCGGAGCAGCTGCGCCGTTTCTTGGCCGAGCTCCAGGTGGAGAAAGATGGCGGCGGCGCGTCGGTATCAGACGACGCGGAGCGGATCGTGGAGCAGGTGCTGCAGAAGAGGCACCACATTACCAAGTTGATCACAAGGTGCACTCTTTCCCTCGAAGACTTCCACCATTACTTGTTCTCTCCCGATCTCAACCCCCCTATTCGAGATCAGGTTCACCAGGACATGACAGCTCCGTTGTCCCATTACTACATATATACTGGCCATAATTCGTACCTGACTGGAAATCAGCTAAGTAGTGACTGCAGTGACGTTCCGATCATAAAGGCACTCAAGAGAGGTGTGAGAGTGGTGGAGCTTGATATTTGGCCAAATTCCACAAAGGACAATGTGCATGTTCTCCATGGAAGGACCTTGACAACCCCTGTTGAACTTATAAAATGCTTGAAATCAATTAAAGAGCATGCCTTTTCTGAATCTCCATACCCTGTCATCATAACTCTTGAAGACCACCTTACGGCTGATCTCCAAGCTAAAGTAGCACAGATGCTCATTCAAACATTTGGGGAAATGTTGTTTTACCCTGAAACCTGTTGTTTGAAAGAGTTACCTTCGCCCGAAGAACTTAAATATCGAATTATTATATCTACAAAACCTCCTGAGGAGTACCTTAAGGATAAAACTTCCAATGAAAATGGACTGGATTCACATAAAAGTGAGGAAGATTTATGGGGGAAGGAACCATCAGAACTTACCCATGAACGGGAAGATGATGATACGAGTGACAGTGATACAAGTGAGGACAACAATAACAACAATCATGGATCATTTTCATCAGTAGAACACGATTACAAATGTCTAATTGCAATCCATGCTGGAAAACCGAAGGGTGGTTTAAAGGACGCGCTAAAAGTTGAACTCGATAAAGTCAGGCGGCTTAGCTTGAGTGAACAAGCACTCGAGAAGGCTGCTGAATCTCATGGAACAGATATTGTTAGATTTACACAGAAGAACATTTTAAGGGTGTATCCAAAAGGTACTCGGTTCAACTCCTCGAACTACAAGCCGCTTGTTGGTTGGATGCATGGTGCTCAAATGGTTGCGTTCAATATGCAGGGATATGGTAGATCTCTTTGGCTGATGCATGGGATGTTTAGAGCCAATGGGGGTTGCGGTTATGTTAAAAAGCCTGATTTTATTATGAAAGCAGATTCCGATAATCAGGTTTTCGATCCTAAAGCAAATCTGCCAGTAAAGAAGACTTTAAAGGTGAAAGTTTATATGGGAGATGGATGGCATTTGGATTTCAAACAAACACACTTTGATTTATATTCTCCACCAGATTTCTATACTAGGGTTGGCATAGCAGGGGTGCCGGCTGATGAAATAATGAAGAAAACGAAGAAAAAAGAGGACGATTGGACACCTGTATGGGAGGAAGAGTTTACATTTCCATTGACAGTTCCGGAGTTGGCTTTGCTTAGAGTTGAGGTACATAGAGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933

Pfam Domains

Protein Families

C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

541

Amino Acids

61.71

Weight (kDa)

6.02

Isoelectric Point (pI)

43.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI-PLC-X	PF00388	115 - 257	3.1e-54	Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y	PF00387	352 - 438	6.8e-29	Phosphatidylinositol-specific phospholipase C, Y domain
C2	PF00168	460 - 538	3.5e-06	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007017)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58690 AT5G58690 AT5G58690 AT5G58700 AT5G58700 AT5G58700
fragaria_vesca	FvH4_3g38660
malus_domestica	MD03G1069000.v1.1
prunus_persica	Prupe.6G056800_v2.0.a1 Prupe.6G056800_v2.0.a1 Prupe.6G056900_v2.0.a1 Prupe.6G056900_v2.0.a1 Prupe.6G056900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0069691
rosa_laevigata	RLG00000036086
rosa_multiflora	Rmu_co8457285.1_g000001 Rmu_sc0000738.1_g000034 Rmu_sc0007688.1_g000005
rosa_roxburghii	Rroxscaffold_1G00011380
rosa_rugosa	Rorug05G0399200
rosa_samantha	Rh5AG456200 Rh5BG475200 Rh5CG498800 Rh5DG486600
rosa_wichuraiana	Rw5G042560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	554
AarI	CACCTGC	1 cut(s)	205
Acc36I	ACCTGC	1 cut(s)	205
AccB1I	GGYRCC	2 cut(s)	231, 1489
AccBSI	CCGCTC	1 cut(s)	202
AccII	CGCG	4 cut(s)	67, 179, 197, 1022
AccIII	TCCGGA	1 cut(s)	1582
AclWI	GGATC	3 cut(s)	212, 949, 1343
AcoI	YGGCCR	3 cut(s)	144, 379, 485
AcsI	RAATTY	1 cut(s)	490
AdeI	CACNNNGTG	1 cut(s)	258
AfaI	GTAC	5 cut(s)	95, 392, 785, 1156, 1611
AfiI	CCNNNNNNNGG	6 cut(s)	142, 634, 879, 880, 1007, 1190
AflII	CTTAAG	1 cut(s)	788
AgsI	TTSAA	9 cut(s)	548, 565, 623, 674, 718, 1034, 1165, 1225, 1426
AjiI	CACGTC	1 cut(s)	73
AjnI	CCWGG	2 cut(s)	153, 336
AluBI	AGCT	7 cut(s)	130, 151, 350, 409, 475, 650, 1059
AluI	AGCT	7 cut(s)	130, 151, 350, 409, 475, 650, 1059
Alw21I	GWGCWC	3 cut(s)	153, 263, 1210
Alw44I	GTGCAC	1 cut(s)	259
AlwI	GGATC	3 cut(s)	212, 949, 1343
AlwNI	CAGNNNCTG	1 cut(s)	220
Ama87I	CYCGRG	1 cut(s)	1076
Aor13HI	TCCGGA	1 cut(s)	1582
AoxI	GGCC	3 cut(s)	144, 379, 485
ApaLI	GTGCAC	1 cut(s)	259
ApeKI	GCWGC	4 cut(s)	127, 130, 220, 1085
ApoI	RAATTY	1 cut(s)	490
AspLEI	GCGC	3 cut(s)	135, 179, 1024
AspS9I	GGNCC	2 cut(s)	108, 528
AsuHPI	GGTGA	3 cut(s)	58, 326, 1399
AsuII	TTCGAA	1 cut(s)	87
AvaI	CYCGRG	1 cut(s)	1076
AvaII	GGWCC	2 cut(s)	108, 528
AxyI	CCTNAGG	1 cut(s)	777
BaeGI	GKGCMC	1 cut(s)	263
BalI	TGGCCA	2 cut(s)	381, 487
BanI	GGYRCC	2 cut(s)	231, 1489
BanII	GRGCYC	1 cut(s)	153
BbsI	GAAGAC	3 cut(s)	282, 630, 1382
Bbv12I	GWGCWC	3 cut(s)	153, 263, 1210
BbvCI	CCTCAGC	1 cut(s)	99
BbvI	GCAGC	4 cut(s)	117, 139, 207, 1072
BccI	CCATC	4 cut(s)	161, 868, 1400, 1404
BceAI	ACGGC	2 cut(s)	120, 651
BcgI	CGANNNNNNTGC	2 cut(s)	1067, 1101
BciT130I	CCWGG	2 cut(s)	155, 338
BciVI	GTATCC	1 cut(s)	1155
BclI	TGATCA	1 cut(s)	249
BfaI	CTAG	2 cut(s)	33, 1470
BfmI	CTRYAG	2 cut(s)	221, 421
BfrI	CTTAAG	1 cut(s)	788
BfuAI	ACCTGC	1 cut(s)	205
BfuI	GTATCC	1 cut(s)	1155
BglII	AGATCT	1 cut(s)	1244
BisI	GCNGC	8 cut(s)	128, 131, 172, 175, 221, 1052, 1086, 1184
BlpI	GCTNAGC	1 cut(s)	1055
BlsI	GCNGC	8 cut(s)	129, 132, 173, 176, 222, 1053, 1087, 1185
Bme1390I	CCNGG	2 cut(s)	155, 338
Bme18I	GGWCC	2 cut(s)	108, 528
BmeT110I	CYCGRG	1 cut(s)	1076
BmgBI	CACGTC	1 cut(s)	73
BmgT120I	GGNCC	2 cut(s)	108, 528
BmiI	GGNNCC	5 cut(s)	109, 110, 233, 858, 1491
BmrFI	CCNGG	2 cut(s)	155, 338
BmsI	GCATC	3 cut(s)	654, 1188, 1248
BpiI	GAAGAC	3 cut(s)	282, 630, 1382
BpmI	CTGGAG	1 cut(s)	137
Bpu10I	CCTNAGC	1 cut(s)	99
Bpu1102I	GCTNAGC	1 cut(s)	1055
Bpu14I	TTCGAA	1 cut(s)	87
BpuEI	CTTGAG	2 cut(s)	434, 1081
BsaJI	CCNNGG	2 cut(s)	65, 520
BsaWI	WCCGGW	1 cut(s)	1582
BsaXI	ACNNNNNCTCC	4 cut(s)	757, 787, 1544, 1574
Bsc4I	CCNNNNNNNGG	6 cut(s)	142, 634, 879, 880, 1007, 1190
Bse118I	RCCGGY	1 cut(s)	1492
Bse1I	ACTGG	4 cut(s)	382, 403, 822, 1367
Bse21I	CCTNAGG	1 cut(s)	777
BseAI	TCCGGA	1 cut(s)	1582
BseBI	CCWGG	2 cut(s)	155, 338
BseDI	CCNNGG	2 cut(s)	65, 520
BseGI	GGATG	4 cut(s)	21, 1203, 1272, 1415
BseLI	CCNNNNNNNGG	6 cut(s)	142, 634, 879, 880, 1007, 1190
BseMII	CTCAG	2 cut(s)	90, 768
BseNI	ACTGG	4 cut(s)	382, 403, 822, 1367
BseRI	GAGGAG	2 cut(s)	794, 1159
BseSI	GKGCMC	1 cut(s)	263
BseXI	GCAGC	4 cut(s)	117, 139, 207, 1072
Bsh1236I	CGCG	4 cut(s)	67, 179, 197, 1022
BshFI	GGCC	3 cut(s)	146, 381, 487
BshNI	GGYRCC	2 cut(s)	231, 1489
BsiHKAI	GWGCWC	3 cut(s)	153, 263, 1210
BsiHKCI	CYCGRG	1 cut(s)	1076
BsiSI	CCGG	2 cut(s)	1493, 1583
BslFI	GGGAC	3 cut(s)	83, 121, 343
BslI	CCNNNNNNNGG	6 cut(s)	142, 634, 879, 880, 1007, 1190
BsmFI	GGGAC	3 cut(s)	83, 121, 343
BsmI	GAATGC	1 cut(s)	83
BsnI	GGCC	3 cut(s)	146, 381, 487
BsoBI	CYCGRG	1 cut(s)	1076
Bsp119I	TTCGAA	1 cut(s)	87
Bsp1286I	GDGCHC	3 cut(s)	153, 263, 1210
Bsp13I	TCCGGA	1 cut(s)	1582
Bsp143I	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
Bsp1720I	GCTNAGC	1 cut(s)	1055
Bsp19I	CCATGG	1 cut(s)	520
BspANI	GGCC	3 cut(s)	146, 381, 487
BspCNI	CTCAG	2 cut(s)	91, 769
BspEI	TCCGGA	1 cut(s)	1582
BspFNI	CGCG	4 cut(s)	67, 179, 197, 1022
BspLI	GGNNCC	5 cut(s)	109, 110, 233, 858, 1491
BspMAI	CTGCAG	2 cut(s)	225, 425
BspMI	ACCTGC	1 cut(s)	205
BspPI	GGATC	3 cut(s)	212, 949, 1343
BspQI	GCTCTTC	1 cut(s)	51
BspT104I	TTCGAA	1 cut(s)	87
BspT107I	GGYRCC	2 cut(s)	231, 1489
BspTI	CTTAAG	1 cut(s)	788
BsrBI	CCGCTC	1 cut(s)	202
BsrFI	RCCGGY	1 cut(s)	1492
BsrI	ACTGG	4 cut(s)	382, 403, 822, 1367
BssAI	RCCGGY	1 cut(s)	1492
BssECI	CCNNGG	2 cut(s)	65, 520
BssMI	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
BssT1I	CCWWGG	1 cut(s)	520
Bst2UI	CCWGG	2 cut(s)	155, 338
Bst4CI	ACNGT	2 cut(s)	905, 1579
Bst6I	CTCTTC	3 cut(s)	51, 221, 1551
BstAFI	CTTAAG	1 cut(s)	788
BstAPI	GCANNNNNTGC	2 cut(s)	220, 989
BstBI	TTCGAA	1 cut(s)	87
BstC8I	GCNNGC	2 cut(s)	582, 1494
BstDEI	CTNAG	5 cut(s)	99, 410, 777, 1055, 1598
BstDSI	CCRYGG	2 cut(s)	65, 520
BstF5I	GGATG	4 cut(s)	21, 1203, 1272, 1415
BstFNI	CGCG	4 cut(s)	67, 179, 197, 1022
BstHHI	GCGC	3 cut(s)	135, 179, 1024
BstKTI	GATC	9 cut(s)	207, 252, 307, 328, 438, 643, 944, 1247, 1351
BstMBI	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
BstMWI	GCNNNNNNNGC	4 cut(s)	220, 229, 656, 989
BstNI	CCWGG	2 cut(s)	155, 338
BstNSI	RCATGY	2 cut(s)	515, 584
BstSCI	CCNGG	2 cut(s)	153, 336
BstSFI	CTRYAG	2 cut(s)	221, 421
BstSLI	GKGCMC	1 cut(s)	263
BstUI	CGCG	4 cut(s)	67, 179, 197, 1022
BstV1I	GCAGC	4 cut(s)	117, 139, 207, 1072
BstV2I	GAAGAC	3 cut(s)	282, 630, 1382
BstX2I	RGATCY	1 cut(s)	1244
BstYI	RGATCY	1 cut(s)	1244
Bsu36I	CCTNAGG	1 cut(s)	777
BsuI	GTATCC	1 cut(s)	1155
BsuRI	GGCC	3 cut(s)	146, 381, 487
BtgI	CCRYGG	2 cut(s)	65, 520
BtrI	CACGTC	1 cut(s)	73
BtsCI	GGATG	4 cut(s)	21, 1203, 1272, 1415
BtsI	GCAGTG	1 cut(s)	430
BtsIMutI	CAGTG	2 cut(s)	430, 910
BveI	ACCTGC	1 cut(s)	205
Cac8I	GCNNGC	2 cut(s)	582, 1494
CaiI	CAGNNNCTG	1 cut(s)	220
CfoI	GCGC	3 cut(s)	135, 179, 1024
Cfr10I	RCCGGY	1 cut(s)	1492
Cfr13I	GGNCC	2 cut(s)	108, 528
Cfr42I	CCGCGG	1 cut(s)	68
CseI	GACGC	3 cut(s)	168, 203, 1028
Csp6I	GTAC	5 cut(s)	94, 391, 784, 1155, 1610
CviQI	GTAC	5 cut(s)	94, 391, 784, 1155, 1610
DdeI	CTNAG	5 cut(s)	99, 410, 777, 1055, 1598
DpnI	GATC	9 cut(s)	206, 251, 306, 327, 437, 642, 943, 1246, 1350
DpnII	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
DraI	TTTAAA	2 cut(s)	1014, 1383
DraIII	CACNNNGTG	1 cut(s)	258
EaeI	YGGCCR	3 cut(s)	144, 379, 485
Eam1104I	CTCTTC	3 cut(s)	51, 221, 1551
EarI	CTCTTC	3 cut(s)	51, 221, 1551
Ecl136II	GAGCTC	1 cut(s)	151
Eco130I	CCWWGG	1 cut(s)	520
Eco24I	GRGCYC	1 cut(s)	153
Eco47I	GGWCC	2 cut(s)	108, 528
Eco53kI	GAGCTC	1 cut(s)	151
Eco81I	CCTNAGG	1 cut(s)	777
Eco88I	CYCGRG	1 cut(s)	1076
EcoICRI	GAGCTC	1 cut(s)	151
EcoO109I	RGGNCCY	2 cut(s)	108, 528
EcoRII	CCWGG	2 cut(s)	153, 336
EcoT14I	CCWWGG	1 cut(s)	520
EcoT22I	ATGCAT	2 cut(s)	1203, 1263
EcoT38I	GRGCYC	1 cut(s)	153
ErhI	CCWWGG	1 cut(s)	520
FalI	AAGNNNNNCTT	2 cut(s)	615, 647
FaqI	GGGAC	3 cut(s)	83, 121, 343
FauI	CCCGC	1 cut(s)	60
FbaI	TGATCA	1 cut(s)	249
Fnu4HI	GCNGC	8 cut(s)	128, 131, 172, 175, 221, 1052, 1086, 1184
FokI	GGATG	4 cut(s)	28, 1210, 1279, 1422
FriOI	GRGCYC	1 cut(s)	153
Fsp4HI	GCNGC	8 cut(s)	128, 131, 172, 175, 221, 1052, 1086, 1184
FspBI	CTAG	2 cut(s)	33, 1470
GlaI	GCGC	3 cut(s)	134, 178, 1023
GluI	GCNGC	8 cut(s)	128, 131, 172, 175, 221, 1052, 1086, 1184
GsuI	CTGGAG	1 cut(s)	137
HaeIII	GGCC	3 cut(s)	146, 381, 487
HapII	CCGG	2 cut(s)	1493, 1583
HgaI	GACGC	3 cut(s)	168, 203, 1028
HhaI	GCGC	3 cut(s)	135, 179, 1024
Hin6I	GCGC	3 cut(s)	133, 177, 1022
HinP1I	GCGC	3 cut(s)	133, 177, 1022
HinfI	GANTC	4 cut(s)	593, 821, 1091, 1328
HpaII	CCGG	2 cut(s)	1493, 1583
HphI	GGTGA	3 cut(s)	58, 326, 1399
Hpy166II	GTNNAC	4 cut(s)	261, 334, 1067, 1563
Hpy188I	TCNGA	5 cut(s)	190, 435, 592, 865, 1333
Hpy188III	TCNNGA	7 cut(s)	302, 323, 451, 620, 776, 1078, 1583
Hpy8I	GTNNAC	4 cut(s)	261, 334, 1067, 1563
Hpy99I	CGWCG	2 cut(s)	184, 197
HpyAV	CCTTC	7 cut(s)	43, 70, 519, 738, 847, 997, 1075
HpyCH4III	ACNGT	2 cut(s)	905, 1579
HpyCH4IV	ACGT	2 cut(s)	72, 429
HpyCH4V	TGCA	8 cut(s)	223, 261, 423, 511, 983, 1201, 1231, 1261
HpyF10VI	GCNNNNNNNGC	4 cut(s)	220, 229, 656, 989
HpyF3I	CTNAG	5 cut(s)	99, 410, 777, 1055, 1598
HpySE526I	ACGT	2 cut(s)	72, 429
HspAI	GCGC	3 cut(s)	133, 177, 1022
KflI	GGGWCCC	1 cut(s)	108
Kpn2I	TCCGGA	1 cut(s)	1582
KroI	GCCGGC	1 cut(s)	1492
KroNI	GCCGGC	1 cut(s)	1494
Ksp22I	TGATCA	1 cut(s)	249
KspI	CCGCGG	1 cut(s)	68
Kzo9I	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
LguI	GCTCTTC	1 cut(s)	51
LmnI	GCTCC	6 cut(s)	124, 156, 199, 211, 355, 472
Lsp1109I	GCAGC	4 cut(s)	117, 139, 207, 1072
LweI	GCATC	3 cut(s)	654, 1188, 1248
MaeI	CTAG	2 cut(s)	33, 1470
MaeII	ACGT	2 cut(s)	72, 429
MaeIII	GTNAC	4 cut(s)	416, 425, 723, 899
MalI	GATC	9 cut(s)	206, 251, 306, 327, 437, 642, 943, 1246, 1350
MbiI	CCGCTC	1 cut(s)	202
MboI	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
MflI	RGATCY	1 cut(s)	1244
MhlI	GDGCHC	3 cut(s)	153, 263, 1210
MlsI	TGGCCA	2 cut(s)	381, 487
MluCI	AATT	5 cut(s)	385, 490, 570, 753, 978
MluNI	TGGCCA	2 cut(s)	381, 487
MmeI	TCCRAC	1 cut(s)	1175
Mox20I	TGGCCA	2 cut(s)	381, 487
Mph1103I	ATGCAT	2 cut(s)	1203, 1263
MroI	TCCGGA	1 cut(s)	1582
MroNI	GCCGGC	1 cut(s)	1492
MscI	TGGCCA	2 cut(s)	381, 487
MseI	TTAA	7 cut(s)	573, 744, 789, 1013, 1136, 1299, 1382
MslI	CAYNNNNRTG	2 cut(s)	831, 1546
Msp20I	TGGCCA	2 cut(s)	381, 487
MspA1I	CMGCKG	3 cut(s)	67, 122, 130
MspCI	CTTAAG	1 cut(s)	788
MspI	CCGG	2 cut(s)	1493, 1583
MspR9I	CCNGG	2 cut(s)	155, 338
Mva1269I	GAATGC	1 cut(s)	83
MvaI	CCWGG	2 cut(s)	155, 338
MvnI	CGCG	4 cut(s)	67, 179, 197, 1022
MwoI	GCNNNNNNNGC	4 cut(s)	220, 229, 656, 989
NaeI	GCCGGC	1 cut(s)	1494
NcoI	CCATGG	1 cut(s)	520
NdeII	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
NgoMIV	GCCGGC	1 cut(s)	1492
NlaIV	GGNNCC	5 cut(s)	109, 110, 233, 858, 1491
NmeAIII	GCCGAG	1 cut(s)	172
NmuCI	GTSAC	3 cut(s)	416, 425, 899
NsiI	ATGCAT	2 cut(s)	1203, 1263
NspI	RCATGY	2 cut(s)	515, 584
NspV	TTCGAA	1 cut(s)	87
PaeI	GCATGC	1 cut(s)	584
PaeR7I	CTCGAG	1 cut(s)	1076
PaqCI	CACCTGC	1 cut(s)	205
PciSI	GCTCTTC	1 cut(s)	51
PctI	GAATGC	1 cut(s)	83
PdiI	GCCGGC	1 cut(s)	1494
PfeI	GAWTC	4 cut(s)	593, 821, 1091, 1328
PkrI	GCNGC	8 cut(s)	129, 132, 173, 176, 222, 1053, 1087, 1185
PpuMI	RGGWCCY	2 cut(s)	108, 528
PsiI	TTATAA	1 cut(s)	554
Psp124BI	GAGCTC	1 cut(s)	153
Psp5II	RGGWCCY	2 cut(s)	108, 528
Psp6I	CCWGG	2 cut(s)	153, 336
PspGI	CCWGG	2 cut(s)	153, 336
PspN4I	GGNNCC	5 cut(s)	109, 110, 233, 858, 1491
PspPI	GGNCC	2 cut(s)	108, 528
PspPPI	RGGWCCY	2 cut(s)	108, 528
PstI	CTGCAG	2 cut(s)	225, 425
PstNI	CAGNNNCTG	1 cut(s)	220
PsuI	RGATCY	1 cut(s)	1244
PvuII	CAGCTG	1 cut(s)	130
RsaI	GTAC	5 cut(s)	95, 392, 785, 1156, 1611
RsaNI	GTAC	5 cut(s)	94, 391, 784, 1155, 1610
RseI	CAYNNNNRTG	2 cut(s)	831, 1546
SacI	GAGCTC	1 cut(s)	153
SacII	CCGCGG	1 cut(s)	68
SapI	GCTCTTC	1 cut(s)	51
SaqAI	TTAA	7 cut(s)	573, 744, 789, 1013, 1136, 1299, 1382
SatI	GCNGC	8 cut(s)	128, 131, 172, 175, 221, 1052, 1086, 1184
Sau3AI	GATC	9 cut(s)	204, 249, 304, 325, 435, 640, 941, 1244, 1348
Sau96I	GGNCC	2 cut(s)	108, 528
ScrFI	CCNGG	2 cut(s)	155, 338
SduI	GDGCHC	3 cut(s)	153, 263, 1210
SfaNI	GCATC	3 cut(s)	654, 1188, 1248
SfcI	CTRYAG	2 cut(s)	221, 421
Sfr274I	CTCGAG	1 cut(s)	1076
Sfr303I	CCGCGG	1 cut(s)	68
SfuI	TTCGAA	1 cut(s)	87
SgrBI	CCGCGG	1 cut(s)	68
SinI	GGWCC	2 cut(s)	108, 528
SlaI	CTCGAG	1 cut(s)	1076
SmiMI	CAYNNNNRTG	2 cut(s)	831, 1546
SmlI	CTYRAG	4 cut(s)	449, 788, 1060, 1076
SmoI	CTYRAG	4 cut(s)	449, 788, 1060, 1076
SphI	GCATGC	1 cut(s)	584
Sse9I	AATT	5 cut(s)	385, 490, 570, 753, 978
SspMI	CTAG	2 cut(s)	33, 1470
SstI	GAGCTC	1 cut(s)	153
StyD4I	CCNGG	2 cut(s)	153, 336
StyI	CCWWGG	1 cut(s)	520
TaaI	ACNGT	2 cut(s)	905, 1579
TaiI	ACGT	2 cut(s)	75, 432
TaqI	TCGA	8 cut(s)	87, 273, 322, 751, 1038, 1077, 1172, 1347
TasI	AATT	5 cut(s)	385, 490, 570, 753, 978
TauI	GCSGC	4 cut(s)	174, 177, 1054, 1186
TfiI	GAWTC	4 cut(s)	593, 821, 1091, 1328
Tru1I	TTAA	7 cut(s)	573, 744, 789, 1013, 1136, 1299, 1382
Tru9I	TTAA	7 cut(s)	573, 744, 789, 1013, 1136, 1299, 1382
TscAI	CASTG	2 cut(s)	430, 910
TseFI	GTSAC	3 cut(s)	416, 425, 899
TseI	GCWGC	4 cut(s)	127, 130, 220, 1085
Tsp45I	GTSAC	3 cut(s)	416, 425, 899
TspDTI	ATGAA	6 cut(s)	822, 891, 939, 1334, 1515, 1523
TspGWI	ACGGA	1 cut(s)	342
TspRI	CASTG	2 cut(s)	430, 910
Vha464I	CTTAAG	1 cut(s)	788
VneI	GTGCAC	1 cut(s)	259
VpaK11BI	GGWCC	2 cut(s)	108, 528
XapI	RAATTY	1 cut(s)	490
XceI	RCATGY	2 cut(s)	515, 584
XhoI	CTCGAG	1 cut(s)	1076
XspI	CTAG	2 cut(s)	33, 1470
Zsp2I	ATGCAT	2 cut(s)	1203, 1263

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.