Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0007017

Physical Location & Seq

Genomic Coordinates Forward (+)

32949153 .. 32953294

Gene Structure 4142 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g38660.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGGGGAGTTACCGGATGTGCGTGTGCTTCACTCGCAAGTTCAGGATCACAGAGGCTGAGCCACCTTCCGACGTGACGGAATACTTCGCTAAGTACGCCGAGGGAGGGACCCACATGACCGCCGAGCAGCTCAGGAACTTTCTGGAGGATGTTCAGGGCTACGGCGGCGCGTCGATCTCGGAGGCCGAGCAGATCGTGGAGCAGATGCTGCAGAAGAGGCACCACCACATTGCCAAGTTGATAACGAGGCGCACGCTCACTCTGGAAGACTTCCACCATTACTTGTTCTCTGCTGATCTCAACCCTCCAATAGAGAACCAGGTTCATCAGGATATGAATGCTCCATTGTCTCATTACTACATATATACTGGACACAATTCATATTTGACTGGAAATCAACTCAGTAGTGACTGCAGTCATGTTCCAATCATCAAGGCACTGAAGAGAGGTGTGAGAGTGGTGGAGCTTGATCTTTGGCCAAATTCGGCAAAGGATGATGTCAATGTTCTTCATGGAAGGACCTTGACAACTCCTGTGGAACTTGTTAAGTGCTTGAAGTCAATTAAAGAGCATGCTTTTACTGAATCTCCATATCCTGTTGTCATAACTCTTGAAGATCACCTTACCCCTGATCTCCAAGCTAAAGTAGCAAAGCTGCTCATGAAGACATTTAAGGAAATGTTGTTTATTCCTGATTCTGAATGTTTCAAAAAATTACCTTCACCAGAAGATCTGAAGTATCGAATAATCATATCTACCAAACCTCCTAAAGAGTACCTTGAGGATAAAAGTGTCAATGACAAGGAAGATAACTTGCACAAGAGAAAAGATAGCGAAGAAGATGAATGGGGGAATGAACCTGCAGAGCCTAAAGAGGAACACGAAGATGTTGGTACAAGTGAAAGTGAAGCAAGTGAACTAAATCAGGATTGCGAGGATAACAATAACTCCAGTCATGGAGCAGAACCTGCATCATACAAACGCCTAATTGCGATCCATGCTGGGAAACCAAGTGGTGGTTTAAAGGAGGCACTTAAAGTTGAGGTTGATCGAGTCAGACGCCTTAGTTTGAGTGAACAAGCACTTGAAAAGGCTGCTGAATGTCATGGAACTGATGTTGTTAGGTTCACCCAGAAGAACATTTTAAGGGTCTATCCAAAAGGAACTCGGTTCAACTCCTCCAACTATAAGCCGCTTATTGGTTGGATGCATGGTGCTCAGATGGTTGCATTTAATATGCAGGGATATGGTAAATCTCTTTGGCTGATGCATGGGATGTTTAGAGCAAATGGGGGATGTGGTTATGTAAAAAAGCCTGATTTTGTCATGAATGATGAGCAGATTTTCGATCCTAAAGCAAAATTGACTGTAAAGAAGACTTTAAAGGTGAAAGTGTATATGGGAGATGGTTGGCATTTGGATTTCAAACAAACACACTTTGATTTATATTCACCACCTGATTTTTACACCAGGGTCGGCATAGCAGGAGTTCCAGCTGATGAGGTAATGAAGAAAACTAAGAAAAAAGAGGATGATTGGACTCCTGTATGGGAAGAAGAGTTTACATTTCCGCTGAGAGTCCCCGAGTTGGCTCTGCTTAGAATCGAGGTACATGAATATGACATGTCTGAGAAAGATGACTTTGGTGGCCAAACTTGTCTGCCAATCTCTGAATTGAAACAAGGAATTCATGCACTCCCACTCTTTAACCGAAAAGGAGAAAAATACAACTCAGTGAGGCTTCTAATGCGATTTGAGTTCACCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0016020 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933

Pfam Domains

Protein Families

C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

589

Amino Acids

67.35

Weight (kDa)

6.08

Isoelectric Point (pI)

46.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI-PLC-X	PF00388	112 - 254	1.6e-53	Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y	PF00387	355 - 441	2e-28	Phosphatidylinositol-specific phospholipase C, Y domain
C2	PF00168	460 - 562	3e-13	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007017)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58690 AT5G58690 AT5G58690 AT5G58700 AT5G58700 AT5G58700
fragaria_vesca	FvH4_3g38660
malus_domestica	MD03G1069000.v1.1
prunus_persica	Prupe.6G056800_v2.0.a1 Prupe.6G056800_v2.0.a1 Prupe.6G056900_v2.0.a1 Prupe.6G056900_v2.0.a1 Prupe.6G056900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0069691
rosa_laevigata	RLG00000036086
rosa_multiflora	Rmu_co8457285.1_g000001 Rmu_sc0000738.1_g000034 Rmu_sc0007688.1_g000005
rosa_roxburghii	Rroxscaffold_1G00011380
rosa_rugosa	Rorug05G0399200
rosa_samantha	Rh5AG456200 Rh5BG475200 Rh5CG498800 Rh5DG486600
rosa_wichuraiana	Rw5G042560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	868, 976
AccB1I	GGYRCC	1 cut(s)	219
AccB7I	CCANNNNNTGG	1 cut(s)	1016
AccII	CGCG	1 cut(s)	170
AciI	CCGC	4 cut(s)	120, 165, 1193, 1571
AclWI	GGATC	3 cut(s)	53, 988, 1343
AcoI	YGGCCR	2 cut(s)	476, 1648
AcsI	RAATTY	2 cut(s)	481, 1686
AcuI	CTGAAG	2 cut(s)	461, 755
AcyI	GRCGYC	1 cut(s)	1060
AfaI	GTAC	4 cut(s)	95, 776, 895, 1611
AfiI	CCNNNNNNNGG	4 cut(s)	105, 1016, 1199, 1588
AflIII	ACRYGT	1 cut(s)	1623
AgsI	TTSAA	7 cut(s)	556, 614, 709, 1088, 1174, 1426, 1678
AjiI	CACGTC	1 cut(s)	73
AjnI	CCWGG	2 cut(s)	318, 1469
AluBI	AGCT	5 cut(s)	130, 466, 641, 655, 1496
AluI	AGCT	5 cut(s)	130, 466, 641, 655, 1496
Alw21I	GWGCWC	1 cut(s)	1219
Alw26I	GTCTC	1 cut(s)	354
AlwI	GGATC	3 cut(s)	53, 988, 1343
AlwNI	CAGNNNCTG	3 cut(s)	56, 208, 968
Ama87I	CYCGRG	1 cut(s)	1583
AoxI	GGCC	3 cut(s)	183, 476, 1648
ApeKI	GCWGC	4 cut(s)	127, 208, 655, 1094
ApoI	RAATTY	2 cut(s)	481, 1686
AspLEI	GCGC	2 cut(s)	170, 252
AspS9I	GGNCC	2 cut(s)	108, 519
AsuHPI	GGTGA	6 cut(s)	611, 714, 1120, 1399, 1443, 1753
AvaI	CYCGRG	1 cut(s)	1583
AvaII	GGWCC	2 cut(s)	108, 519
BalI	TGGCCA	2 cut(s)	478, 1650
BanI	GGYRCC	1 cut(s)	219
BbsI	GAAGAC	3 cut(s)	273, 671, 1382
Bbv12I	GWGCWC	1 cut(s)	1219
BbvI	GCAGC	4 cut(s)	139, 195, 642, 1081
BccI	CCATC	2 cut(s)	1216, 1400
BceAI	ACGGC	1 cut(s)	178
BciT130I	CCWGG	2 cut(s)	320, 1471
BcoDI	GTCTC	1 cut(s)	354
BfmI	CTRYAG	3 cut(s)	209, 412, 861
BfuAI	ACCTGC	2 cut(s)	868, 976
BglII	AGATCT	1 cut(s)	730
BisI	GCNGC	6 cut(s)	128, 166, 209, 656, 1095, 1193
BlpI	GCTNAGC	1 cut(s)	57
BlsI	GCNGC	6 cut(s)	129, 167, 210, 657, 1096, 1194
Bme1390I	CCNGG	2 cut(s)	320, 1471
Bme18I	GGWCC	2 cut(s)	108, 519
BmeT110I	CYCGRG	1 cut(s)	1583
BmgBI	CACGTC	1 cut(s)	73
BmgT120I	GGNCC	2 cut(s)	108, 519
BmiI	GGNNCC	3 cut(s)	109, 110, 221
BmrFI	CCNGG	2 cut(s)	320, 1471
BmsI	GCATC	4 cut(s)	195, 980, 1197, 1257
BoxI	GACNNNNGTC	1 cut(s)	414
BpiI	GAAGAC	3 cut(s)	273, 671, 1382
BpmI	CTGGAG	2 cut(s)	164, 934
Bpu10I	CCTNAGC	1 cut(s)	131
Bpu1102I	GCTNAGC	1 cut(s)	57
BpuEI	CTTGAG	1 cut(s)	800
BsaHI	GRCGYC	1 cut(s)	1060
BsaJI	CCNNGG	2 cut(s)	99, 1470
BsaWI	WCCGGW	1 cut(s)	12
BsaXI	ACNNNNNCTCC	6 cut(s)	571, 601, 748, 778, 932, 962
Bsc4I	CCNNNNNNNGG	4 cut(s)	105, 1016, 1199, 1588
Bse1I	ACTGG	3 cut(s)	373, 394, 951
Bse3DI	GCAATG	1 cut(s)	228
BseBI	CCWGG	2 cut(s)	320, 1471
BseDI	CCNNGG	2 cut(s)	99, 1470
BseGI	GGATG	7 cut(s)	21, 154, 499, 1212, 1281, 1301, 1537
BseLI	CCNNNNNNNGG	4 cut(s)	105, 1016, 1199, 1588
BseMI	GCAATG	1 cut(s)	228
BseMII	CTCAG	7 cut(s)	48, 145, 415, 1232, 1565, 1620, 1746
BseNI	ACTGG	3 cut(s)	373, 394, 951
BseRI	GAGGAG	1 cut(s)	1168
BseXI	GCAGC	4 cut(s)	139, 195, 642, 1081
BseYI	CCCAGC	1 cut(s)	1001
Bsh1236I	CGCG	1 cut(s)	170
BshFI	GGCC	3 cut(s)	185, 478, 1650
BshNI	GGYRCC	1 cut(s)	219
BsiHKAI	GWGCWC	1 cut(s)	1219
BsiHKCI	CYCGRG	1 cut(s)	1583
BsiSI	CCGG	1 cut(s)	13
BslFI	GGGAC	2 cut(s)	121, 1565
BslI	CCNNNNNNNGG	4 cut(s)	105, 1016, 1199, 1588
BsmAI	GTCTC	1 cut(s)	354
BsmFI	GGGAC	2 cut(s)	121, 1565
BsmI	GAATGC	1 cut(s)	343
BsnI	GGCC	3 cut(s)	185, 478, 1650
BsoBI	CYCGRG	1 cut(s)	1583
Bsp1286I	GDGCHC	1 cut(s)	1219
Bsp1720I	GCTNAGC	1 cut(s)	57
BspACI	CCGC	4 cut(s)	120, 165, 1193, 1571
BspANI	GGCC	3 cut(s)	185, 478, 1650
BspCNI	CTCAG	7 cut(s)	49, 144, 414, 1231, 1566, 1621, 1745
BspFNI	CGCG	1 cut(s)	170
BspHI	TCATGA	2 cut(s)	660, 1326
BspLI	GGNNCC	3 cut(s)	109, 110, 221
BspMAI	CTGCAG	3 cut(s)	213, 416, 865
BspMI	ACCTGC	2 cut(s)	868, 976
BspPI	GGATC	3 cut(s)	53, 988, 1343
BspT107I	GGYRCC	1 cut(s)	219
BsrDI	GCAATG	1 cut(s)	228
BsrI	ACTGG	3 cut(s)	373, 394, 951
BssECI	CCNNGG	2 cut(s)	99, 1470
BssNI	GRCGYC	1 cut(s)	1060
Bst2UI	CCWGG	2 cut(s)	320, 1471
Bst4CI	ACNGT	1 cut(s)	1369
Bst6I	CTCTTC	3 cut(s)	209, 437, 1551
BstACI	GRCGYC	1 cut(s)	1060
BstAPI	GCANNNNNTGC	2 cut(s)	208, 968
BstC8I	GCNNGC	2 cut(s)	254, 573
BstF5I	GGATG	7 cut(s)	21, 154, 499, 1212, 1281, 1301, 1537
BstFNI	CGCG	1 cut(s)	170
BstHHI	GCGC	2 cut(s)	170, 252
BstMAI	GTCTC	1 cut(s)	354
BstMWI	GCNNNNNNNGC	9 cut(s)	33, 95, 165, 208, 217, 647, 968, 998, 1747
BstNI	CCWGG	2 cut(s)	320, 1471
BstNSI	RCATGY	2 cut(s)	575, 1627
BstPAI	GACNNNNGTC	1 cut(s)	414
BstSCI	CCNGG	2 cut(s)	318, 1469
BstSFI	CTRYAG	3 cut(s)	209, 412, 861
BstUI	CGCG	1 cut(s)	170
BstV1I	GCAGC	4 cut(s)	139, 195, 642, 1081
BstV2I	GAAGAC	3 cut(s)	273, 671, 1382
BstX2I	RGATCY	1 cut(s)	730
BstYI	RGATCY	1 cut(s)	730
BsuRI	GGCC	3 cut(s)	185, 478, 1650
BtrI	CACGTC	1 cut(s)	73
BtsCI	GGATG	7 cut(s)	21, 154, 499, 1212, 1281, 1301, 1537
BtsIMutI	CAGTG	2 cut(s)	437, 1740
BveI	ACCTGC	2 cut(s)	868, 976
Cac8I	GCNNGC	2 cut(s)	254, 573
CaiI	CAGNNNCTG	3 cut(s)	56, 208, 968
CciI	TCATGA	2 cut(s)	660, 1326
CfoI	GCGC	2 cut(s)	170, 252
Cfr13I	GGNCC	2 cut(s)	108, 519
CseI	GACGC	2 cut(s)	159, 1068
CsiI	ACCWGGT	1 cut(s)	318
Csp6I	GTAC	4 cut(s)	94, 775, 894, 1610
CspCI	CAANNNNNGTGG	2 cut(s)	516, 551
CviQI	GTAC	4 cut(s)	94, 775, 894, 1610
DraI	TTTAAA	2 cut(s)	1023, 1383
EaeI	YGGCCR	2 cut(s)	476, 1648
Eam1104I	CTCTTC	3 cut(s)	209, 437, 1551
EarI	CTCTTC	3 cut(s)	209, 437, 1551
Eco47I	GGWCC	2 cut(s)	108, 519
Eco57I	CTGAAG	2 cut(s)	461, 755
Eco88I	CYCGRG	1 cut(s)	1583
EcoO109I	RGGNCCY	2 cut(s)	108, 519
EcoRI	GAATTC	1 cut(s)	1686
EcoRII	CCWGG	2 cut(s)	318, 1469
EcoT22I	ATGCAT	2 cut(s)	1212, 1272
FalI	AAGNNNNNCTT	4 cut(s)	606, 638, 762, 794
FaqI	GGGAC	2 cut(s)	121, 1565
Fnu4HI	GCNGC	6 cut(s)	128, 166, 209, 656, 1095, 1193
FokI	GGATG	7 cut(s)	28, 161, 506, 1219, 1288, 1308, 1544
Fsp4HI	GCNGC	6 cut(s)	128, 166, 209, 656, 1095, 1193
GlaI	GCGC	2 cut(s)	169, 251
GluI	GCNGC	6 cut(s)	128, 166, 209, 656, 1095, 1193
GsaI	CCCAGC	1 cut(s)	1005
GsuI	CTGGAG	2 cut(s)	164, 934
HaeIII	GGCC	3 cut(s)	185, 478, 1650
HapII	CCGG	1 cut(s)	13
HgaI	GACGC	2 cut(s)	159, 1068
HhaI	GCGC	2 cut(s)	170, 252
Hin1I	GRCGYC	1 cut(s)	1060
Hin6I	GCGC	2 cut(s)	168, 250
HinP1I	GCGC	2 cut(s)	168, 250
HinfI	GANTC	6 cut(s)	584, 695, 1053, 1540, 1578, 1602
HpaII	CCGG	1 cut(s)	13
HphI	GGTGA	6 cut(s)	611, 714, 1120, 1399, 1443, 1753
Hpy166II	GTNNAC	5 cut(s)	917, 1076, 1128, 1563, 1761
Hpy188I	TCNGA	8 cut(s)	70, 181, 700, 735, 1058, 1221, 1630, 1672
Hpy8I	GTNNAC	5 cut(s)	917, 1076, 1128, 1563, 1761
Hpy99I	CGWCG	2 cut(s)	74, 175
HpyAV	CCTTC	3 cut(s)	75, 510, 729
HpyCH4III	ACNGT	1 cut(s)	1369
HpyCH4IV	ACGT	1 cut(s)	72
HpyF10VI	GCNNNNNNNGC	9 cut(s)	33, 95, 165, 208, 217, 647, 968, 998, 1747
HpySE526I	ACGT	1 cut(s)	72
Hsp92I	GRCGYC	1 cut(s)	1060
HspAI	GCGC	2 cut(s)	168, 250
KflI	GGGWCCC	1 cut(s)	108
LmnI	GCTCC	4 cut(s)	199, 346, 463, 959
Lsp1109I	GCAGC	4 cut(s)	139, 195, 642, 1081
LweI	GCATC	4 cut(s)	195, 980, 1197, 1257
MabI	ACCWGGT	1 cut(s)	318
MaeII	ACGT	1 cut(s)	72
MaeIII	GTNAC	3 cut(s)	8, 73, 407
MflI	RGATCY	1 cut(s)	730
MhlI	GDGCHC	1 cut(s)	1219
MlsI	TGGCCA	2 cut(s)	478, 1650
MluCI	AATT	8 cut(s)	376, 481, 561, 713, 987, 1361, 1673, 1686
MluNI	TGGCCA	2 cut(s)	478, 1650
MlyI	GAGTC	3 cut(s)	1062, 1534, 1587
MmeI	TCCRAC	3 cut(s)	93, 1184, 1206
Mox20I	TGGCCA	2 cut(s)	478, 1650
Mph1103I	ATGCAT	2 cut(s)	1212, 1272
MscI	TGGCCA	2 cut(s)	478, 1650
MseI	TTAA	9 cut(s)	546, 564, 672, 1022, 1035, 1145, 1233, 1382, 1707
MslI	CAYNNNNRTG	2 cut(s)	885, 1617
Msp20I	TGGCCA	2 cut(s)	478, 1650
MspA1I	CMGCKG	2 cut(s)	1496, 1573
MspI	CCGG	1 cut(s)	13
MspR9I	CCNGG	2 cut(s)	320, 1471
Mva1269I	GAATGC	1 cut(s)	343
MvaI	CCWGG	2 cut(s)	320, 1471
MvnI	CGCG	1 cut(s)	170
MwoI	GCNNNNNNNGC	9 cut(s)	33, 95, 165, 208, 217, 647, 968, 998, 1747
NlaIV	GGNNCC	3 cut(s)	109, 110, 221
NmeAIII	GCCGAG	3 cut(s)	124, 148, 211
NmuCI	GTSAC	2 cut(s)	73, 407
NsiI	ATGCAT	2 cut(s)	1212, 1272
NspI	RCATGY	2 cut(s)	575, 1627
PaeI	GCATGC	1 cut(s)	575
PagI	TCATGA	2 cut(s)	660, 1326
PciI	ACATGT	1 cut(s)	1623
PctI	GAATGC	1 cut(s)	343
PfeI	GAWTC	3 cut(s)	584, 695, 1602
PflMI	CCANNNNNTGG	1 cut(s)	1016
PkrI	GCNGC	6 cut(s)	129, 167, 210, 657, 1096, 1194
PleI	GAGTC	3 cut(s)	1061, 1534, 1586
PpsI	GAGTC	3 cut(s)	1061, 1534, 1586
PpuMI	RGGWCCY	2 cut(s)	108, 519
PscI	ACATGT	1 cut(s)	1623
PshAI	GACNNNNGTC	1 cut(s)	414
Psp5II	RGGWCCY	2 cut(s)	108, 519
Psp6I	CCWGG	2 cut(s)	318, 1469
PspFI	CCCAGC	1 cut(s)	1001
PspGI	CCWGG	2 cut(s)	318, 1469
PspN4I	GGNNCC	3 cut(s)	109, 110, 221
PspPI	GGNCC	2 cut(s)	108, 519
PspPPI	RGGWCCY	2 cut(s)	108, 519
PstI	CTGCAG	3 cut(s)	213, 416, 865
PstNI	CAGNNNCTG	3 cut(s)	56, 208, 968
PsuI	RGATCY	1 cut(s)	730
PvuII	CAGCTG	1 cut(s)	1496
RsaI	GTAC	4 cut(s)	95, 776, 895, 1611
RsaNI	GTAC	4 cut(s)	94, 775, 894, 1610
RseI	CAYNNNNRTG	2 cut(s)	885, 1617
SaqAI	TTAA	9 cut(s)	546, 564, 672, 1022, 1035, 1145, 1233, 1382, 1707
SatI	GCNGC	6 cut(s)	128, 166, 209, 656, 1095, 1193
Sau96I	GGNCC	2 cut(s)	108, 519
SchI	GAGTC	3 cut(s)	1062, 1534, 1587
ScrFI	CCNGG	2 cut(s)	320, 1471
SduI	GDGCHC	1 cut(s)	1219
SexAI	ACCWGGT	1 cut(s)	318
SfaNI	GCATC	4 cut(s)	195, 980, 1197, 1257
SfcI	CTRYAG	3 cut(s)	209, 412, 861
SinI	GGWCC	2 cut(s)	108, 519
SmiMI	CAYNNNNRTG	2 cut(s)	885, 1617
SmlI	CTYRAG	1 cut(s)	779
SmoI	CTYRAG	1 cut(s)	779
SphI	GCATGC	1 cut(s)	575
Sse9I	AATT	8 cut(s)	376, 481, 561, 713, 987, 1361, 1673, 1686
SsiI	CCGC	4 cut(s)	120, 165, 1193, 1571
StyD4I	CCNGG	2 cut(s)	318, 1469
TaaI	ACNGT	1 cut(s)	1369
TaiI	ACGT	1 cut(s)	75
TaqI	TCGA	5 cut(s)	173, 742, 1051, 1347, 1605
TasI	AATT	8 cut(s)	376, 481, 561, 713, 987, 1361, 1673, 1686
TauI	GCSGC	2 cut(s)	168, 1195
TfiI	GAWTC	3 cut(s)	584, 695, 1602
Tru1I	TTAA	9 cut(s)	546, 564, 672, 1022, 1035, 1145, 1233, 1382, 1707
Tru9I	TTAA	9 cut(s)	546, 564, 672, 1022, 1035, 1145, 1233, 1382, 1707
TscAI	CASTG	2 cut(s)	444, 1740
TseFI	GTSAC	2 cut(s)	73, 407
TseI	GCWGC	4 cut(s)	127, 208, 655, 1094
Tsp45I	GTSAC	2 cut(s)	73, 407
TspGWI	ACGGA	1 cut(s)	92
TspRI	CASTG	2 cut(s)	444, 1740
Van91I	CCANNNNNTGG	1 cut(s)	1016
VpaK11BI	GGWCC	2 cut(s)	108, 519
XapI	RAATTY	2 cut(s)	481, 1686
XceI	RCATGY	2 cut(s)	575, 1627
Zsp2I	ATGCAT	2 cut(s)	1212, 1272

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.