AT5G58750

3-oxo-Delta(45)-steroid

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015921
Physical Location & Seq
Forward (+)
23728588 .. 23730142
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58750.2

Sequence Viewer

Length: 1161 bp
ATGGGGTCTGAAAATGGCAGCTTGATGAGAAGAAACGAAGTAGACGAGAATGTAGCGTTAATCTTTGGCGTCACCGGGCTTGTGGGTCGAGAGATTGTTAAGACGCTATTAATGTCTAAACCAGGATGGAGAATCTACGGCGTAGCGCGTAACCCGGAGATCAATTCCATGACGAAGATGTACAACTTCATCTCCTGCGATCTGCTTAACGCATCTGAGACTAAACAGAGGTTGTCTCCATTACAAGACATCGTGAGTCACGTGTTTTGGGTCACGTGGTCTGGTGAGTTTCCATTGGATACCGACGAATGTTGCGTACAGAACAAGACGATGCTGATGAACGCTTTGGACGCGATTCTCCCAAACGCTAAGAGGTTAAAGCATTTCTCGCTTCAAACGGGGATGAAGCATTATGTGTCTTTGGTGGAAGAGACAATGGCTCGTGGAGAAGGTTCGAGTTTGTATTATTACAGCGAGGAGTGTCCAAGAAAGAGCTCTGGGAAGAATTTCTACTATGTTTTGGAGGACTTGCTGAAGGAGAAGATCACTCGTAGTTCCGTTGTTTGGTCGGTTCAAAGACCTGGTTTGCTAATGGGAAGCTCTTCAAGAACTCTGTACAACTTCATGGGAAGTCTTTGTGTTTATGGAGCGATGTGTAAGTATTTGAATCTTCCTTTTGTGTTTGGAGGAACAAGAGAATGTTGGGAAGAGAGTTACATAGATGGCTCTGATTCGAATTTAGTCGCGGAGCAGCATATATTCGCTGCAACAAGTGGGAAAGTACGCGAGAAAGGGGAAGCTTTTAACGCCATTAATGGAGTAGGGTTTACTTGGAAGGAGATTTGGCCGGAAATAGGGAAGAAACTTGGTGTACAAGTTAACGAAACAACCATGTTTGATGAAGGTTTCTGGTTTGGGAGAGAGATGGTTGAGAGAAAACATGTGTGGGATGAGATTGTTGTGAAGGAGAAGCTTGTTAGGACAGAGATTGAGGATTTGGCGAATTGGTATTTCTTGGATGCTCTGTTTAGATGTCCGTTTAAGCTTCTTGGGAAAAGAGAAAAAGTAGATAGGTTTGGGTTTAAGAGGAAATATAGAACCCTAGATTCGGTTTTGTATTGGATTGATGTGATGAGAGATGAAAAACTCATTCCTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

386

Amino Acids

44.5

Weight (kDa)

6.96

Isoelectric Point (pI)

38.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 64 - 284 1.3e-11 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58750 AT5G58750
fragaria_vesca FvH4_3g38520
malus_domestica MD03G1071000.v1.1 MD11G1075000.v1.1
prunus_persica Prupe.6G057800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0069421
rosa_laevigata RLG00000036051
rosa_multiflora Rmu_sc0002658.1_g000023
rosa_roxburghii Rroxscaffold_1G00011540
rosa_rugosa Rorug05G0397500
rosa_samantha Rh5BG473500 Rh5CG496000 Rh5DG485500
rosa_wichuraiana Rw5G042410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 42
AccII CGCG 4 cut(s) 148, 353, 746, 786
AciI CCGC 1 cut(s) 746
AcoI YGGCCR 1 cut(s) 845
AcsI RAATTY 2 cut(s) 505, 736
AcuI CTGAAG 1 cut(s) 554
AcvI CACGTG 2 cut(s) 262, 276
AcyI GRCGYC 1 cut(s) 69
AfaI GTAC 5 cut(s) 182, 318, 617, 783, 873
AfiI CCNNNNNNNGG 3 cut(s) 564, 854, 1108
AflIII ACRYGT 2 cut(s) 261, 940
AgsI TTSAA 4 cut(s) 395, 575, 606, 667
AjnI CCWGG 2 cut(s) 121, 580
AluBI AGCT 6 cut(s) 21, 495, 600, 800, 973, 1045
AluI AGCT 6 cut(s) 21, 495, 600, 800, 973, 1045
Alw21I GWGCWC 1 cut(s) 497
Alw26I GTCTC 3 cut(s) 212, 240, 425
AoxI GGCC 1 cut(s) 845
ApeKI GCWGC 3 cut(s) 18, 751, 764
ApoI RAATTY 2 cut(s) 505, 736
AseI ATTAAT 2 cut(s) 110, 813
Asp700I GAANNNNTTC 2 cut(s) 506, 601
AspLEI GCGC 1 cut(s) 148
AsuC2I CCSGG 2 cut(s) 76, 155
AsuHPI GGTGA 2 cut(s) 64, 296
AsuII TTCGAA 1 cut(s) 734
BanII GRGCYC 1 cut(s) 497
BarI GAAGNNNNNNTAC 2 cut(s) 494, 526
BauI CACGAG 1 cut(s) 441
BbrPI CACGTG 2 cut(s) 262, 276
Bbv12I GWGCWC 1 cut(s) 497
BbvI GCAGC 3 cut(s) 30, 751, 763
BccI CCATC 3 cut(s) 120, 716, 919
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 2 cut(s) 123, 582
BciVI GTATCC 1 cut(s) 292
BcnI CCSGG 2 cut(s) 76, 155
BcoDI GTCTC 3 cut(s) 212, 240, 425
BfaI CTAG 1 cut(s) 1103
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 3 cut(s) 19, 752, 765
BlsI GCNGC 3 cut(s) 20, 753, 766
Bme1390I CCNGG 4 cut(s) 76, 123, 155, 582
BmrFI CCNGG 4 cut(s) 76, 123, 155, 582
BmsI GCATC 3 cut(s) 221, 321, 1009
BplI GAGNNNNNCTC 2 cut(s) 220, 252
Bpu14I TTCGAA 1 cut(s) 734
BpuMI CCSGG 2 cut(s) 76, 155
BsaAI YACGTR 2 cut(s) 262, 276
BsaHI GRCGYC 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 3 cut(s) 564, 854, 1108
BseBI CCWGG 2 cut(s) 123, 582
BseGI GGATG 4 cut(s) 131, 408, 955, 1024
BseLI CCNNNNNNNGG 3 cut(s) 564, 854, 1108
BseMII CTCAG 1 cut(s) 207
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 3 cut(s) 30, 751, 763
Bsh1236I CGCG 4 cut(s) 148, 353, 746, 786
BshFI GGCC 1 cut(s) 847
BsiHKAI GWGCWC 1 cut(s) 497
BsiSI CCGG 3 cut(s) 75, 155, 848
BslI CCNNNNNNNGG 3 cut(s) 564, 854, 1108
BsmAI GTCTC 3 cut(s) 212, 240, 425
BsnI GGCC 1 cut(s) 847
Bsp119I TTCGAA 1 cut(s) 734
Bsp1286I GDGCHC 1 cut(s) 497
Bsp1407I TGTACA 3 cut(s) 180, 615, 871
Bsp143I GATC 3 cut(s) 159, 199, 543
BspACI CCGC 1 cut(s) 746
BspANI GGCC 1 cut(s) 847
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 4 cut(s) 148, 353, 746, 786
BspQI GCTCTTC 1 cut(s) 607
BspT104I TTCGAA 1 cut(s) 734
BsrGI TGTACA 3 cut(s) 180, 615, 871
BssMI GATC 3 cut(s) 159, 199, 543
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 441
Bst2BI CACGAG 1 cut(s) 441
Bst2UI CCWGG 2 cut(s) 123, 582
Bst6I CTCTTC 3 cut(s) 423, 607, 702
BstACI GRCGYC 1 cut(s) 69
BstAUI TGTACA 3 cut(s) 180, 615, 871
BstBAI YACGTR 2 cut(s) 262, 276
BstBI TTCGAA 1 cut(s) 734
BstDEI CTNAG 2 cut(s) 216, 369
BstF5I GGATG 4 cut(s) 131, 408, 955, 1024
BstFNI CGCG 4 cut(s) 148, 353, 746, 786
BstHHI GCGC 1 cut(s) 148
BstKTI GATC 3 cut(s) 162, 202, 546
BstMAI GTCTC 3 cut(s) 212, 240, 425
BstMBI GATC 3 cut(s) 159, 199, 543
BstMWI GCNNNNNNNGC 3 cut(s) 350, 388, 806
BstNI CCWGG 2 cut(s) 123, 582
BstNSI RCATGY 1 cut(s) 944
BstSCI CCNGG 4 cut(s) 74, 121, 153, 580
BstUI CGCG 4 cut(s) 148, 353, 746, 786
BstV1I GCAGC 3 cut(s) 30, 751, 763
BsuI GTATCC 1 cut(s) 292
BsuRI GGCC 1 cut(s) 847
BtgZI GCGATG 1 cut(s) 665
BtsCI GGATG 4 cut(s) 131, 408, 955, 1024
CfoI GCGC 1 cut(s) 148
CseI GACGC 3 cut(s) 58, 112, 359
CsiI ACCWGGT 1 cut(s) 580
Csp6I GTAC 5 cut(s) 181, 317, 616, 782, 872
CviAII CATG 4 cut(s) 169, 625, 892, 941
CviQI GTAC 5 cut(s) 181, 317, 616, 782, 872
DdeI CTNAG 2 cut(s) 216, 369
DpnI GATC 3 cut(s) 161, 201, 545
DpnII GATC 3 cut(s) 159, 199, 543
EaeI YGGCCR 1 cut(s) 845
Eam1104I CTCTTC 3 cut(s) 423, 607, 702
EarI CTCTTC 3 cut(s) 423, 607, 702
Ecl136II GAGCTC 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 497
Eco53kI GAGCTC 1 cut(s) 495
Eco57I CTGAAG 1 cut(s) 554
Eco72I CACGTG 2 cut(s) 262, 276
EcoICRI GAGCTC 1 cut(s) 495
EcoRII CCWGG 2 cut(s) 121, 580
EcoT38I GRGCYC 1 cut(s) 497
FaeI CATG 4 cut(s) 172, 628, 895, 944
FatI CATG 4 cut(s) 168, 624, 891, 940
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 3 cut(s) 19, 752, 765
FokI GGATG 4 cut(s) 138, 415, 962, 1031
FriOI GRGCYC 1 cut(s) 497
Fsp4HI GCNGC 3 cut(s) 19, 752, 765
FspBI CTAG 1 cut(s) 1103
GlaI GCGC 1 cut(s) 147
GluI GCNGC 3 cut(s) 19, 752, 765
HaeIII GGCC 1 cut(s) 847
HapII CCGG 3 cut(s) 75, 155, 848
HgaI GACGC 3 cut(s) 58, 112, 359
HhaI GCGC 1 cut(s) 148
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 4 cut(s) 172, 628, 895, 944
Hin6I GCGC 1 cut(s) 146
HinP1I GCGC 1 cut(s) 146
HincII GTYRAC 1 cut(s) 880
HindII GTYRAC 1 cut(s) 880
HindIII AAGCTT 3 cut(s) 798, 971, 1043
HinfI GANTC 6 cut(s) 132, 256, 355, 667, 731, 1106
HpaI GTTAAC 1 cut(s) 880
HpaII CCGG 3 cut(s) 75, 155, 848
HphI GGTGA 2 cut(s) 64, 296
Hpy166II GTNNAC 4 cut(s) 43, 828, 872, 880
Hpy188I TCNGA 3 cut(s) 10, 217, 730
Hpy188III TCNNGA 3 cut(s) 89, 253, 606
Hpy8I GTNNAC 4 cut(s) 43, 828, 872, 880
Hpy99I CGWCG 1 cut(s) 308
HpyAV CCTTC 5 cut(s) 443, 529, 829, 896, 958
HpyCH4IV ACGT 2 cut(s) 261, 275
HpyCH4V TGCA 1 cut(s) 767
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 388, 806
HpyF3I CTNAG 2 cut(s) 216, 369
HpySE526I ACGT 2 cut(s) 261, 275
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 4 cut(s) 172, 628, 895, 944
HspAI GCGC 1 cut(s) 146
KspAI GTTAAC 1 cut(s) 880
Kzo9I GATC 3 cut(s) 159, 199, 543
LguI GCTCTTC 1 cut(s) 607
LmnI GCTCC 2 cut(s) 647, 748
Lsp1109I GCAGC 3 cut(s) 30, 751, 763
LweI GCATC 3 cut(s) 221, 321, 1009
MabI ACCWGGT 1 cut(s) 580
MaeI CTAG 1 cut(s) 1103
MaeII ACGT 2 cut(s) 261, 275
MaeIII GTNAC 5 cut(s) 70, 149, 257, 271, 713
MalI GATC 3 cut(s) 161, 201, 545
MboI GATC 3 cut(s) 159, 199, 543
MboII GAAGA 9 cut(s) 42, 187, 440, 514, 553, 594, 662, 719, 871
MhlI GDGCHC 1 cut(s) 497
MluCI AATT 4 cut(s) 163, 505, 736, 1003
MlyI GAGTC 1 cut(s) 265
MnlI CCTC 7 cut(s) 222, 366, 469, 517, 680, 985, 1080
MroXI GAANNNNTTC 2 cut(s) 506, 601
MspI CCGG 3 cut(s) 75, 155, 848
MspR9I CCNGG 4 cut(s) 76, 123, 155, 582
MvaI CCWGG 2 cut(s) 123, 582
MvnI CGCG 4 cut(s) 148, 353, 746, 786
MwoI GCNNNNNNNGC 3 cut(s) 350, 388, 806
NciI CCSGG 2 cut(s) 76, 155
NdeII GATC 3 cut(s) 159, 199, 543
NlaIII CATG 4 cut(s) 172, 628, 895, 944
NmuCI GTSAC 3 cut(s) 70, 257, 271
NspI RCATGY 1 cut(s) 944
NspV TTCGAA 1 cut(s) 734
PciI ACATGT 1 cut(s) 940
PciSI GCTCTTC 1 cut(s) 607
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 258, 312
PdmI GAANNNNTTC 2 cut(s) 506, 601
PfeI GAWTC 5 cut(s) 132, 355, 667, 731, 1106
PkrI GCNGC 3 cut(s) 20, 753, 766
PleI GAGTC 1 cut(s) 264
PmaCI CACGTG 2 cut(s) 262, 276
PmlI CACGTG 2 cut(s) 262, 276
PpsI GAGTC 1 cut(s) 264
Ppu21I YACGTR 2 cut(s) 262, 276
PscI ACATGT 1 cut(s) 940
PshBI ATTAAT 2 cut(s) 110, 813
Psp124BI GAGCTC 1 cut(s) 497
Psp6I CCWGG 2 cut(s) 121, 580
PspCI CACGTG 2 cut(s) 262, 276
PspGI CCWGG 2 cut(s) 121, 580
RsaI GTAC 5 cut(s) 182, 318, 617, 783, 873
RsaNI GTAC 5 cut(s) 181, 317, 616, 782, 872
SacI GAGCTC 1 cut(s) 497
SapI GCTCTTC 1 cut(s) 607
SatI GCNGC 3 cut(s) 19, 752, 765
Sau3AI GATC 3 cut(s) 159, 199, 543
SchI GAGTC 1 cut(s) 265
ScrFI CCNGG 4 cut(s) 76, 123, 155, 582
SduI GDGCHC 1 cut(s) 497
SexAI ACCWGGT 1 cut(s) 580
SfaNI GCATC 3 cut(s) 221, 321, 1009
SfuI TTCGAA 1 cut(s) 734
Sse9I AATT 4 cut(s) 163, 505, 736, 1003
SsiI CCGC 1 cut(s) 746
SspMI CTAG 1 cut(s) 1103
SstI GAGCTC 1 cut(s) 497
StyD4I CCNGG 4 cut(s) 74, 121, 153, 580
TaiI ACGT 2 cut(s) 264, 278
TaqI TCGA 3 cut(s) 88, 455, 734
TasI AATT 4 cut(s) 163, 505, 736, 1003
TatI WGTACW 3 cut(s) 180, 615, 871
TfiI GAWTC 5 cut(s) 132, 355, 667, 731, 1106
TseFI GTSAC 3 cut(s) 70, 257, 271
TseI GCWGC 3 cut(s) 18, 751, 764
Tsp45I GTSAC 3 cut(s) 70, 257, 271
TspDTI ATGAA 6 cut(s) 178, 353, 419, 613, 915, 1155
TspGWI ACGGA 2 cut(s) 547, 1026
VspI ATTAAT 2 cut(s) 110, 813
XapI RAATTY 2 cut(s) 505, 736
XceI RCATGY 1 cut(s) 944
XmiI GTMKAC 1 cut(s) 42
XmnI GAANNNNTTC 2 cut(s) 506, 601
XspI CTAG 1 cut(s) 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.