MD11G1075000.v1.1

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015921
Physical Location & Seq
Forward (+)
6354212 .. 6355372
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1075000.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGCAGCTGAAGAAACTGAGAACCCAGAGGCTGGCAATGGCATTGAACATGTAGCAGCCATTTTCGGGGTCACCGGGCTAGTTGGGAAGGTGCTGGCCAGAGAGCTGGTCACAAAACCCAAATGGAAGGTTTACGGCATAGCTCGTAATCCGGAGATCATGCCACCTATTGATCAACGTTCTTCAAACTTCCATTTCATCCAATGTGATCTGCTAAACCGTTCGGAAACCGAAGAACGGCTGTCACTGTTGCAAGATGTTACTCATGTGTTTTGGCTCACCTGGGCAAGCCGATACCAGTTGGATAGCGTTGAGTGTTGTGAGCAGAATAAGGCCATGATGTCGAATGCATTGGATGCCATTGTTCCGAAAGCGAAGGCATTGAAGCATGTTTCTCTCCAGACAGGGATGAAGCATTACGTGTCGTTGCTAGGGCCTTTCGATGATGAAGCAGAAGCTCGGTACTACGATGAAGATTGCCCGAGAGTGGATAAAGGGTGCAACTTTTACTATGTCCTAGAAGACTTGCTAAAGGAGAGGCTGGTTGGTAAGGTAGCTTGGTCGGTACACAGGCCTGGTTTGTTAATTGGTAGTTCTCGTAGGACTGTGTACAATTTTATGGGGAGTTTATGTGTTTTCGGAACCATTTGTAAGCATTTGAATCTGCCTTTTGTGTTTGGAGGGACAAAAAGGTGTTGGGAAGAGACTTGTATCGATGGCTCGGATGCCAGGCTAGTAGCTCAACAGCACATTTGGGCAGCAACCAATGTTGATTTATACTCACATTCCACTGATGGTCAGTCATACAATGCAATCAATGGGCCAAGTTTTACATGGAAGGAGATCTGGACGGCTCTTGGAGAGAAATTCGGAGTAGAAGTAGTACACGGAAGTGCATTTTCGGAGGAGTTCTGGTATTCGAAATCCATGGCTGACAAAAAAGAAGTATGGAAAGAAATTGTAGCAGAGAAGGGACTGATTCAAACTGAGATGGAAAATTTGGCCAATTGGGAATTCTTGGATCTCTTGTTTCGTTGTCCGTTTAAGCTACTGGGAACCCGAAACAAAGTTGATCGACTAGGTTTTACTGTAGGGTATAAGACACTGGATTCAATCTTGTACTGGGTAGATTGTATGAGAGATGAAAAGTTAATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

387

Amino Acids

43.97

Weight (kDa)

5.66

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 21 - 122 3.3e-06 NAD dependent epimerase/dehydratase family
PRISE PF22917 75 - 327 5.5e-16 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58750 AT5G58750
fragaria_vesca FvH4_3g38520
malus_domestica MD03G1071000.v1.1 MD11G1075000.v1.1
prunus_persica Prupe.6G057800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0069421
rosa_laevigata RLG00000036051
rosa_multiflora Rmu_sc0002658.1_g000023
rosa_roxburghii Rroxscaffold_1G00011540
rosa_rugosa Rorug05G0397500
rosa_samantha Rh5BG473500 Rh5CG496000 Rh5DG485500
rosa_wichuraiana Rw5G042410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 32
AccIII TCCGGA 1 cut(s) 151
AclI AACGTT 1 cut(s) 178
AclWI GGATC 1 cut(s) 1029
AcoI YGGCCR 2 cut(s) 96, 1002
AcsI RAATTY 3 cut(s) 866, 997, 1013
AcuI CTGAAG 1 cut(s) 30
AfaI GTAC 5 cut(s) 464, 567, 611, 885, 1121
AfiI CCNNNNNNNGG 4 cut(s) 32, 66, 237, 487
AflIII ACRYGT 2 cut(s) 49, 420
AgsI TTSAA 6 cut(s) 47, 186, 385, 661, 983, 1113
AjnI CCWGG 3 cut(s) 281, 574, 728
AjuI GAANNNNNNNTTGG 2 cut(s) 998, 1030
AleI CACNNNNGTG 1 cut(s) 891
AluBI AGCT 7 cut(s) 8, 106, 143, 458, 557, 740, 1048
AluI AGCT 7 cut(s) 8, 106, 143, 458, 557, 740, 1048
Alw26I GTCTC 1 cut(s) 698
AlwI GGATC 1 cut(s) 1029
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 1 cut(s) 481
Aor13HI TCCGGA 1 cut(s) 151
AoxI GGCC 6 cut(s) 96, 333, 434, 572, 821, 1002
ApeKI GCWGC 3 cut(s) 5, 56, 758
ApoI RAATTY 3 cut(s) 866, 997, 1013
AspS9I GGNCC 2 cut(s) 434, 821
AsuC2I CCSGG 1 cut(s) 76
AsuHPI GGTGA 2 cut(s) 64, 271
AsuII TTCGAA 1 cut(s) 920
AvaI CYCGRG 1 cut(s) 481
BalI TGGCCA 2 cut(s) 98, 1004
BbsI GAAGAC 1 cut(s) 528
BbvI GCAGC 3 cut(s) 17, 68, 770
BccI CCATC 3 cut(s) 710, 788, 985
BceAI ACGGC 3 cut(s) 151, 254, 867
BciT130I CCWGG 3 cut(s) 283, 576, 730
BclI TGATCA 1 cut(s) 172
BcnI CCSGG 1 cut(s) 76
BcoDI GTCTC 1 cut(s) 698
BfaI CTAG 5 cut(s) 80, 431, 518, 734, 1079
BfmI CTRYAG 1 cut(s) 1089
BglII AGATCT 1 cut(s) 843
BisI GCNGC 3 cut(s) 6, 57, 759
BlsI GCNGC 3 cut(s) 7, 58, 760
Bme1390I CCNGG 4 cut(s) 76, 283, 576, 730
BmeT110I CYCGRG 1 cut(s) 481
BmgT120I GGNCC 2 cut(s) 434, 821
BmiI GGNNCC 2 cut(s) 643, 1057
BmrFI CCNGG 4 cut(s) 76, 283, 576, 730
BmrI ACTGGG 2 cut(s) 1061, 1132
BmsI GCATC 2 cut(s) 346, 715
BmuI ACTGGG 2 cut(s) 1061, 1132
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 383
Bpu14I TTCGAA 1 cut(s) 920
BpuMI CCSGG 1 cut(s) 76
Bsa29I ATCGAT 1 cut(s) 714
BsaAI YACGTR 1 cut(s) 421
BsaJI CCNNGG 2 cut(s) 282, 927
BsaWI WCCGGW 1 cut(s) 151
BsaXI ACNNNNNCTCC 6 cut(s) 616, 646, 864, 894, 899, 929
Bsc4I CCNNNNNNNGG 4 cut(s) 32, 66, 237, 487
Bse1I ACTGG 4 cut(s) 298, 1056, 1110, 1127
Bse3DI GCAATG 1 cut(s) 43
BseAI TCCGGA 1 cut(s) 151
BseBI CCWGG 3 cut(s) 283, 576, 730
BseCI ATCGAT 1 cut(s) 714
BseDI CCNNGG 2 cut(s) 282, 927
BseGI GGATG 4 cut(s) 198, 361, 414, 730
BseLI CCNNNNNNNGG 4 cut(s) 32, 66, 237, 487
BseMI GCAATG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 9, 978
BseNI ACTGG 4 cut(s) 298, 1056, 1110, 1127
BseRI GAGGAG 1 cut(s) 920
BseXI GCAGC 3 cut(s) 17, 68, 770
BshFI GGCC 6 cut(s) 98, 335, 436, 574, 823, 1004
BshVI ATCGAT 1 cut(s) 714
BsiHKCI CYCGRG 1 cut(s) 481
BsiSI CCGG 2 cut(s) 75, 152
BslFI GGGAC 2 cut(s) 697, 987
BslI CCNNNNNNNGG 4 cut(s) 32, 66, 237, 487
BsmAI GTCTC 1 cut(s) 698
BsmFI GGGAC 2 cut(s) 697, 987
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 6 cut(s) 98, 335, 436, 574, 823, 1004
BsoBI CYCGRG 1 cut(s) 481
Bsp119I TTCGAA 1 cut(s) 920
Bsp13I TCCGGA 1 cut(s) 151
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
Bsp19I CCATGG 1 cut(s) 927
BspANI GGCC 6 cut(s) 98, 335, 436, 574, 823, 1004
BspCNI CTCAG 2 cut(s) 10, 979
BspDI ATCGAT 1 cut(s) 714
BspEI TCCGGA 1 cut(s) 151
BspLI GGNNCC 2 cut(s) 643, 1057
BspPI GGATC 1 cut(s) 1029
BspT104I TTCGAA 1 cut(s) 920
BsrDI GCAATG 1 cut(s) 43
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 4 cut(s) 298, 1056, 1110, 1127
BssECI CCNNGG 2 cut(s) 282, 927
BssMI GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
BssT1I CCWWGG 1 cut(s) 927
Bst2UI CCWGG 3 cut(s) 283, 576, 730
Bst4CI ACNGT 4 cut(s) 221, 249, 607, 1090
Bst6I CTCTTC 1 cut(s) 696
BstAPI GCANNNNNTGC 1 cut(s) 356
BstAUI TGTACA 1 cut(s) 609
BstBAI YACGTR 1 cut(s) 421
BstBI TTCGAA 1 cut(s) 920
BstC8I GCNNGC 3 cut(s) 34, 96, 289
BstDEI CTNAG 2 cut(s) 18, 987
BstDSI CCRYGG 1 cut(s) 927
BstEII GGTNACC 1 cut(s) 70
BstF5I GGATG 4 cut(s) 198, 361, 414, 730
BstKTI GATC 6 cut(s) 159, 175, 211, 846, 1024, 1075
BstMAI GTCTC 1 cut(s) 698
BstMBI GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNI CCWGG 3 cut(s) 283, 576, 730
BstNSI RCATGY 2 cut(s) 53, 392
BstPI GGTNACC 1 cut(s) 70
BstSCI CCNGG 4 cut(s) 74, 281, 574, 728
BstSFI CTRYAG 1 cut(s) 1089
BstV1I GCAGC 3 cut(s) 17, 68, 770
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 2 cut(s) 843, 1021
BstXI CCANNNNNNTGG 1 cut(s) 106
BstYI RGATCY 2 cut(s) 843, 1021
Bsu15I ATCGAT 1 cut(s) 714
BsuRI GGCC 6 cut(s) 98, 335, 436, 574, 823, 1004
BsuTUI ATCGAT 1 cut(s) 714
BtgI CCRYGG 1 cut(s) 927
BtsCI GGATG 4 cut(s) 198, 361, 414, 730
BtsIMutI CAGTG 3 cut(s) 245, 789, 1103
Cac8I GCNNGC 3 cut(s) 34, 96, 289
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 434, 821
ClaI ATCGAT 1 cut(s) 714
Csp6I GTAC 5 cut(s) 463, 566, 610, 884, 1120
CviAII CATG 7 cut(s) 50, 160, 266, 337, 389, 834, 928
CviQI GTAC 5 cut(s) 463, 566, 610, 884, 1120
DdeI CTNAG 2 cut(s) 18, 987
DpnI GATC 6 cut(s) 158, 174, 210, 845, 1023, 1074
DpnII GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
EaeI YGGCCR 2 cut(s) 96, 1002
Eam1104I CTCTTC 1 cut(s) 696
EarI CTCTTC 1 cut(s) 696
Eco130I CCWWGG 1 cut(s) 927
Eco147I AGGCCT 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 481
Eco91I GGTNACC 1 cut(s) 70
EcoO109I RGGNCCY 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 70
EcoRI GAATTC 1 cut(s) 1013
EcoRII CCWGG 3 cut(s) 281, 574, 728
EcoT14I CCWWGG 1 cut(s) 927
EcoT22I ATGCAT 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 927
FaeI CATG 7 cut(s) 53, 163, 269, 340, 392, 837, 931
FaqI GGGAC 2 cut(s) 697, 987
FatI CATG 7 cut(s) 49, 159, 265, 336, 388, 833, 927
FbaI TGATCA 1 cut(s) 172
Fnu4HI GCNGC 3 cut(s) 6, 57, 759
FokI GGATG 4 cut(s) 185, 368, 421, 737
Fsp4HI GCNGC 3 cut(s) 6, 57, 759
FspBI CTAG 5 cut(s) 80, 431, 518, 734, 1079
GluI GCNGC 3 cut(s) 6, 57, 759
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 6 cut(s) 98, 335, 436, 574, 823, 1004
HapII CCGG 2 cut(s) 75, 152
Hin1II CATG 7 cut(s) 53, 163, 269, 340, 392, 837, 931
HinfI GANTC 3 cut(s) 661, 979, 1109
HpaII CCGG 2 cut(s) 75, 152
HphI GGTGA 2 cut(s) 64, 271
Hpy166II GTNNAC 4 cut(s) 133, 568, 610, 886
Hpy188I TCNGA 6 cut(s) 226, 369, 641, 724, 872, 904
Hpy188III TCNNGA 3 cut(s) 152, 400, 847
Hpy8I GTNNAC 4 cut(s) 133, 568, 610, 886
HpyAV CCTTC 5 cut(s) 82, 121, 370, 832, 964
HpyCH4III ACNGT 4 cut(s) 221, 249, 607, 1090
HpyCH4IV ACGT 2 cut(s) 178, 420
HpyCH4V TGCA 5 cut(s) 253, 350, 501, 812, 896
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 2 cut(s) 18, 987
HpySE526I ACGT 2 cut(s) 178, 420
Hsp92II CATG 7 cut(s) 53, 163, 269, 340, 392, 837, 931
Kpn2I TCCGGA 1 cut(s) 151
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
Lsp1109I GCAGC 3 cut(s) 17, 68, 770
LweI GCATC 2 cut(s) 346, 715
MaeI CTAG 5 cut(s) 80, 431, 518, 734, 1079
MaeII ACGT 2 cut(s) 178, 420
MaeIII GTNAC 4 cut(s) 70, 109, 243, 259
MalI GATC 6 cut(s) 158, 174, 210, 845, 1023, 1074
MboI GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
MboII GAAGA 6 cut(s) 23, 174, 245, 485, 533, 713
MfeI CAATTG 1 cut(s) 1006
MflI RGATCY 2 cut(s) 843, 1021
MlsI TGGCCA 2 cut(s) 98, 1004
MluCI AATT 7 cut(s) 585, 613, 866, 957, 997, 1006, 1013
MluNI TGGCCA 2 cut(s) 98, 1004
MmeI TCCRAC 1 cut(s) 282
MnlI CCTC 4 cut(s) 22, 531, 674, 898
Mox20I TGGCCA 2 cut(s) 98, 1004
Mph1103I ATGCAT 1 cut(s) 352
MroI TCCGGA 1 cut(s) 151
MscI TGGCCA 2 cut(s) 98, 1004
MseI TTAA 4 cut(s) 584, 1044, 1151, 1159
MslI CAYNNNNRTG 1 cut(s) 891
Msp20I TGGCCA 2 cut(s) 98, 1004
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 75, 152
MspR9I CCNGG 4 cut(s) 76, 283, 576, 730
MunI CAATTG 1 cut(s) 1006
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 3 cut(s) 283, 576, 730
MwoI GCNNNNNNNGC 1 cut(s) 356
NciI CCSGG 1 cut(s) 76
NcoI CCATGG 1 cut(s) 927
NdeII GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
NlaIII CATG 7 cut(s) 53, 163, 269, 340, 392, 837, 931
NlaIV GGNNCC 2 cut(s) 643, 1057
NmuCI GTSAC 3 cut(s) 70, 109, 243
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 2 cut(s) 53, 392
NspV TTCGAA 1 cut(s) 920
OliI CACNNNNGTG 1 cut(s) 891
PceI AGGCCT 1 cut(s) 574
PciI ACATGT 1 cut(s) 49
PctI GAATGC 1 cut(s) 352
PfeI GAWTC 3 cut(s) 661, 979, 1109
PflMI CCANNNNNTGG 1 cut(s) 32
PkrI GCNGC 3 cut(s) 7, 58, 760
Ppu21I YACGTR 1 cut(s) 421
PscI ACATGT 1 cut(s) 49
Psp1406I AACGTT 1 cut(s) 178
Psp6I CCWGG 3 cut(s) 281, 574, 728
PspEI GGTNACC 1 cut(s) 70
PspGI CCWGG 3 cut(s) 281, 574, 728
PspN4I GGNNCC 2 cut(s) 643, 1057
PspPI GGNCC 2 cut(s) 434, 821
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 2 cut(s) 843, 1021
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 464, 567, 611, 885, 1121
RsaNI GTAC 5 cut(s) 463, 566, 610, 884, 1120
RseI CAYNNNNRTG 1 cut(s) 891
SaqAI TTAA 4 cut(s) 584, 1044, 1151, 1159
SatI GCNGC 3 cut(s) 6, 57, 759
Sau3AI GATC 6 cut(s) 156, 172, 208, 843, 1021, 1072
Sau96I GGNCC 2 cut(s) 434, 821
ScrFI CCNGG 4 cut(s) 76, 283, 576, 730
SfaNI GCATC 2 cut(s) 346, 715
SfcI CTRYAG 1 cut(s) 1089
SfuI TTCGAA 1 cut(s) 920
SmiMI CAYNNNNRTG 1 cut(s) 891
Sse9I AATT 7 cut(s) 585, 613, 866, 957, 997, 1006, 1013
SseBI AGGCCT 1 cut(s) 574
SspMI CTAG 5 cut(s) 80, 431, 518, 734, 1079
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 4 cut(s) 74, 281, 574, 728
StyI CCWWGG 1 cut(s) 927
TaaI ACNGT 4 cut(s) 221, 249, 607, 1090
TaiI ACGT 2 cut(s) 181, 423
TaqI TCGA 5 cut(s) 344, 441, 714, 920, 1075
TasI AATT 7 cut(s) 585, 613, 866, 957, 997, 1006, 1013
TatI WGTACW 3 cut(s) 609, 883, 1119
TfiI GAWTC 3 cut(s) 661, 979, 1109
Tru1I TTAA 4 cut(s) 584, 1044, 1151, 1159
Tru9I TTAA 4 cut(s) 584, 1044, 1151, 1159
TscAI CASTG 3 cut(s) 252, 796, 1110
TseFI GTSAC 3 cut(s) 70, 109, 243
TseI GCWGC 3 cut(s) 5, 56, 758
Tsp45I GTSAC 3 cut(s) 70, 109, 243
TspDTI ATGAA 5 cut(s) 187, 425, 462, 486, 1158
TspGWI ACGGA 2 cut(s) 903, 1029
TspRI CASTG 3 cut(s) 252, 796, 1110
Van91I CCANNNNNTGG 1 cut(s) 32
XapI RAATTY 3 cut(s) 866, 997, 1013
XceI RCATGY 2 cut(s) 53, 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 831
XspI CTAG 5 cut(s) 80, 431, 518, 734, 1079
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.