RLG00000036051

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015921
Physical Location & Seq
Reverse (-)
75330553 .. 75331704
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036051

Sequence Viewer

Length: 1152 bp
ATGGCAGCTGAAGAAACTAAAAACCCAGAGGCTGTTGCCATTGATAATGTTGCAGCCATTTTTGGGGTAACTGGGTTAGTTGGGAAGGTACTTGCCAGAAAGCTAATATCAACACCCAAATGGAAGGTTTATGGTATAGCTCGAGCTCCTGATCATCAGATCATAACCATTGATCAAAGTTCTTCAAACTTCCATTTCATCCAATGTGATTTGCTAAACCCTTTGGAAACCCAGAAAAGACTCCCATTGTTACAAGATGTGACTCATGTCTTTTGGGTCACTTGGGCAAGCCAGTACCAATTGGATAGCATTGAGTGCTGTGACCAAAACAGAGCCATGATGTCCAATGCATTGGATGCTATACTTCCTGGTGCCATGGCATTGAGGCATGTTTCTCTTCAGACTGGGATGAAGCATTATGTGTCACTGCAAGGCCCTTTTGATGAAACTGAAGAAGCTCGATACTACAATGAGGAGTCTCCGCGAGTAAATCGAAGTTACAACTTTTACTATGTTCTTGAAGACTTGCTCAAGGAGAGGCTACATGGTAAGATAGGTTGGTCTGTGCATAGGCCTGGTTTGTTAATCGGTAGTTCTTCTAGGACTTTGTACAATTTTATGGGTAGTTTATGTGTTTATGGGACTATTTGTAAGCATTTAAATCTCCCATTTGTGTTTGGAGGGACAAAAAAGTGTTGGGAAGAGACTTGTATTGATGGCTCAGATGCAAGGCTAGTAGCTGATCAACACATTTGGGCAGTCACAAATGTTGATTTGTATGATCATAGCAATTCGTTTAATGCGATCAACGGTCCAAGTTTTACATGGAAGGAGATCTGGAAAGCTGTTGCAGAAAAGCTTGGAGTACAAGTATCAGAAGAACACACATTTTCTGAGGACTTTTGGTATTCAAAAGCCATGGCTGACAAGCAACAAGTGTGGGAACAAATTGTAGCAGAAAAAGGGCTGGTTGAAACTGAGATGGACAATTTGGCCAATTGGGATTTCTTGGATGTTCTATTTCGCTGCCCGGTTAAGATTTTGGGGACTCGAGAAAAAGTTGATGGACTTGGTTTTACTGTAAGGTACAAGACAGTGGATTCAATCATGTATTGGATAGATTGTATGAGAGATGAGAAACTAATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

384

Amino Acids

43.71

Weight (kDa)

5.51

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 76 - 329 6.1e-14 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58750 AT5G58750
fragaria_vesca FvH4_3g38520
malus_domestica MD03G1071000.v1.1 MD11G1075000.v1.1
prunus_persica Prupe.6G057800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0069421
rosa_laevigata RLG00000036051
rosa_multiflora Rmu_sc0002658.1_g000023
rosa_roxburghii Rroxscaffold_1G00011540
rosa_rugosa Rorug05G0397500
rosa_samantha Rh5BG473500 Rh5CG496000 Rh5DG485500
rosa_wichuraiana Rw5G042410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 371
AccII CGCG 1 cut(s) 484
AciI CCGC 1 cut(s) 482
AcoI YGGCCR 1 cut(s) 993
AcuI CTGAAG 3 cut(s) 30, 383, 471
AfaI GTAC 5 cut(s) 90, 296, 611, 867, 1088
AfiI CCNNNNNNNGG 1 cut(s) 63
AgsI TTSAA 5 cut(s) 186, 521, 912, 974, 1104
AjnI CCWGG 2 cut(s) 367, 574
AjuI GAANNNNNNNTTGG 2 cut(s) 989, 1021
AluBI AGCT 8 cut(s) 8, 103, 140, 146, 458, 740, 845, 859
AluI AGCT 8 cut(s) 8, 103, 140, 146, 458, 740, 845, 859
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 2 cut(s) 483, 698
AlwNI CAGNNNCTG 1 cut(s) 32
Ama87I CYCGRG 2 cut(s) 141, 1050
AoxI GGCC 3 cut(s) 433, 572, 993
ApeKI GCWGC 3 cut(s) 5, 53, 1026
AspS9I GGNCC 2 cut(s) 434, 812
AsuC2I CCSGG 1 cut(s) 1031
AvaI CYCGRG 2 cut(s) 141, 1050
AvaII GGWCC 1 cut(s) 812
BalI TGGCCA 1 cut(s) 995
BanI GGYRCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 148
BbsI GAAGAC 1 cut(s) 528
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 17, 65, 1013
BccI CCATC 3 cut(s) 710, 976, 1058
BciT130I CCWGG 2 cut(s) 369, 576
BclI TGATCA 4 cut(s) 151, 172, 742, 781
BcnI CCSGG 1 cut(s) 1031
BcoDI GTCTC 2 cut(s) 483, 698
BfaI CTAG 2 cut(s) 600, 734
BglII AGATCT 1 cut(s) 834
BisI GCNGC 3 cut(s) 6, 54, 1027
BlsI GCNGC 3 cut(s) 7, 55, 1028
Bme1390I CCNGG 3 cut(s) 369, 576, 1031
Bme18I GGWCC 1 cut(s) 812
BmeT110I CYCGRG 2 cut(s) 141, 1050
BmgT120I GGNCC 2 cut(s) 434, 812
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 3 cut(s) 369, 576, 1031
BmrI ACTGGG 2 cut(s) 81, 414
BmsI GCATC 2 cut(s) 346, 715
BmuI ACTGGG 2 cut(s) 81, 414
BoxI GACNNNNGTC 1 cut(s) 266
BpiI GAAGAC 1 cut(s) 528
BpuEI CTTGAG 1 cut(s) 515
BpuMI CCSGG 1 cut(s) 1031
BsaJI CCNNGG 2 cut(s) 375, 918
BsaXI ACNNNNNCTCC 2 cut(s) 855, 885
Bsc4I CCNNNNNNNGG 1 cut(s) 63
Bse1I ACTGG 3 cut(s) 76, 292, 409
BseBI CCWGG 2 cut(s) 369, 576
BseDI CCNNGG 2 cut(s) 375, 918
BseGI GGATG 4 cut(s) 198, 361, 414, 1018
BseLI CCNNNNNNNGG 1 cut(s) 63
BseMII CTCAG 3 cut(s) 735, 885, 969
BseNI ACTGG 3 cut(s) 76, 292, 409
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 3 cut(s) 17, 65, 1013
Bsh1236I CGCG 1 cut(s) 484
BshFI GGCC 3 cut(s) 435, 574, 995
BshNI GGYRCC 1 cut(s) 371
BsiHKAI GWGCWC 1 cut(s) 148
BsiHKCI CYCGRG 2 cut(s) 141, 1050
BsiSI CCGG 1 cut(s) 1031
BslFI GGGAC 3 cut(s) 655, 697, 1060
BslI CCNNNNNNNGG 1 cut(s) 63
BsmAI GTCTC 2 cut(s) 483, 698
BsmFI GGGAC 3 cut(s) 655, 697, 1060
BsnI GGCC 3 cut(s) 435, 574, 995
BsoBI CYCGRG 2 cut(s) 141, 1050
Bsp1286I GDGCHC 1 cut(s) 148
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
Bsp19I CCATGG 2 cut(s) 375, 918
BspACI CCGC 1 cut(s) 482
BspANI GGCC 3 cut(s) 435, 574, 995
BspCNI CTCAG 3 cut(s) 734, 886, 970
BspFNI CGCG 1 cut(s) 484
BspLI GGNNCC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 371
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 3 cut(s) 76, 292, 409
BssECI CCNNGG 2 cut(s) 375, 918
BssMI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
BssT1I CCWWGG 2 cut(s) 375, 918
Bst2UI CCWGG 2 cut(s) 369, 576
Bst4CI ACNGT 3 cut(s) 812, 1081, 1096
Bst6I CTCTTC 2 cut(s) 402, 696
BstAPI GCANNNNNTGC 2 cut(s) 315, 356
BstAUI TGTACA 1 cut(s) 609
BstC8I GCNNGC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 721, 894, 978
BstDSI CCRYGG 2 cut(s) 375, 918
BstF5I GGATG 4 cut(s) 198, 361, 414, 1018
BstFNI CGCG 1 cut(s) 484
BstKTI GATC 7 cut(s) 154, 162, 175, 745, 784, 807, 837
BstMAI GTCTC 2 cut(s) 483, 698
BstMBI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
BstMWI GCNNNNNNNGC 2 cut(s) 315, 356
BstNI CCWGG 2 cut(s) 369, 576
BstNSI RCATGY 1 cut(s) 392
BstPAI GACNNNNGTC 1 cut(s) 266
BstSCI CCNGG 3 cut(s) 367, 574, 1029
BstUI CGCG 1 cut(s) 484
BstV1I GCAGC 3 cut(s) 17, 65, 1013
BstV2I GAAGAC 1 cut(s) 528
BstX2I RGATCY 1 cut(s) 834
BstXI CCANNNNNNTGG 1 cut(s) 352
BstYI RGATCY 1 cut(s) 834
BsuRI GGCC 3 cut(s) 435, 574, 995
BtgI CCRYGG 2 cut(s) 375, 918
BtsCI GGATG 4 cut(s) 198, 361, 414, 1018
BtsI GCAGTG 1 cut(s) 425
BtsIMutI CAGTG 2 cut(s) 425, 1101
Cac8I GCNNGC 1 cut(s) 289
CaiI CAGNNNCTG 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 434, 812
Csp6I GTAC 5 cut(s) 89, 295, 610, 866, 1087
CspCI CAANNNNNGTGG 2 cut(s) 920, 955
CviAII CATG 8 cut(s) 266, 337, 376, 389, 545, 825, 919, 1108
CviQI GTAC 5 cut(s) 89, 295, 610, 866, 1087
DdeI CTNAG 3 cut(s) 721, 894, 978
DpnI GATC 7 cut(s) 153, 161, 174, 744, 783, 806, 836
DpnII GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
DraI TTTAAA 1 cut(s) 660
EaeI YGGCCR 1 cut(s) 993
Eam1104I CTCTTC 2 cut(s) 402, 696
EarI CTCTTC 2 cut(s) 402, 696
Ecl136II GAGCTC 1 cut(s) 146
Eco130I CCWWGG 2 cut(s) 375, 918
Eco147I AGGCCT 1 cut(s) 574
Eco24I GRGCYC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 812
Eco53kI GAGCTC 1 cut(s) 146
Eco57I CTGAAG 3 cut(s) 30, 383, 471
Eco88I CYCGRG 2 cut(s) 141, 1050
EcoICRI GAGCTC 1 cut(s) 146
EcoO109I RGGNCCY 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 367, 574
EcoT14I CCWWGG 2 cut(s) 375, 918
EcoT22I ATGCAT 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 148
ErhI CCWWGG 2 cut(s) 375, 918
FaeI CATG 8 cut(s) 269, 340, 379, 392, 548, 828, 922, 1111
FaqI GGGAC 3 cut(s) 655, 697, 1060
FatI CATG 8 cut(s) 265, 336, 375, 388, 544, 824, 918, 1107
FbaI TGATCA 4 cut(s) 151, 172, 742, 781
Fnu4HI GCNGC 3 cut(s) 6, 54, 1027
FokI GGATG 4 cut(s) 185, 368, 421, 1025
FriOI GRGCYC 1 cut(s) 148
Fsp4HI GCNGC 3 cut(s) 6, 54, 1027
FspBI CTAG 2 cut(s) 600, 734
GluI GCNGC 3 cut(s) 6, 54, 1027
HaeIII GGCC 3 cut(s) 435, 574, 995
HapII CCGG 1 cut(s) 1031
Hin1II CATG 8 cut(s) 269, 340, 379, 392, 548, 828, 922, 1111
HindIII AAGCTT 1 cut(s) 857
HinfI GANTC 5 cut(s) 240, 262, 476, 1048, 1100
HpaII CCGG 1 cut(s) 1031
Hpy188I TCNGA 5 cut(s) 159, 402, 724, 877, 895
Hpy188III TCNNGA 4 cut(s) 149, 518, 838, 1052
HpyAV CCTTC 3 cut(s) 79, 118, 823
HpyCH4III ACNGT 3 cut(s) 812, 1081, 1096
HpyCH4V TGCA 6 cut(s) 53, 350, 430, 568, 728, 851
HpyF10VI GCNNNNNNNGC 2 cut(s) 315, 356
HpyF3I CTNAG 3 cut(s) 721, 894, 978
Hsp92II CATG 8 cut(s) 269, 340, 379, 392, 548, 828, 922, 1111
Ksp22I TGATCA 4 cut(s) 151, 172, 742, 781
Kzo9I GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
LmnI GCTCC 1 cut(s) 151
Lsp1109I GCAGC 3 cut(s) 17, 65, 1013
LweI GCATC 2 cut(s) 346, 715
MaeI CTAG 2 cut(s) 600, 734
MaeIII GTNAC 8 cut(s) 67, 249, 259, 277, 320, 423, 497, 760
MalI GATC 7 cut(s) 153, 161, 174, 744, 783, 806, 836
MboI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
MboII GAAGA 8 cut(s) 23, 174, 389, 464, 533, 588, 713, 890
MfeI CAATTG 2 cut(s) 299, 997
MflI RGATCY 1 cut(s) 834
MhlI GDGCHC 1 cut(s) 148
MlsI TGGCCA 1 cut(s) 995
MluCI AATT 6 cut(s) 299, 613, 790, 948, 988, 997
MluNI TGGCCA 1 cut(s) 995
MlyI GAGTC 4 cut(s) 234, 256, 485, 1042
MnlI CCTC 6 cut(s) 22, 378, 466, 531, 674, 889
Mox20I TGGCCA 1 cut(s) 995
Mph1103I ATGCAT 1 cut(s) 352
MscI TGGCCA 1 cut(s) 995
MseI TTAA 5 cut(s) 584, 659, 798, 1035, 1150
MslI CAYNNNNRTG 1 cut(s) 118
Msp20I TGGCCA 1 cut(s) 995
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 1031
MspR9I CCNGG 3 cut(s) 369, 576, 1031
MunI CAATTG 2 cut(s) 299, 997
MvaI CCWGG 2 cut(s) 369, 576
MvnI CGCG 1 cut(s) 484
MwoI GCNNNNNNNGC 2 cut(s) 315, 356
NciI CCSGG 1 cut(s) 1031
NcoI CCATGG 2 cut(s) 375, 918
NdeII GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
NlaIII CATG 8 cut(s) 269, 340, 379, 392, 548, 828, 922, 1111
NlaIV GGNNCC 1 cut(s) 373
NmuCI GTSAC 5 cut(s) 259, 277, 320, 423, 760
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 1 cut(s) 392
PaeR7I CTCGAG 2 cut(s) 141, 1050
PceI AGGCCT 1 cut(s) 574
PcsI WCGNNNNNNNCGW 1 cut(s) 800
PfeI GAWTC 1 cut(s) 1100
PkrI GCNGC 3 cut(s) 7, 55, 1028
PleI GAGTC 4 cut(s) 234, 256, 484, 1042
PpsI GAGTC 4 cut(s) 234, 256, 484, 1042
PshAI GACNNNNGTC 1 cut(s) 266
Psp124BI GAGCTC 1 cut(s) 148
Psp6I CCWGG 2 cut(s) 367, 574
PspGI CCWGG 2 cut(s) 367, 574
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 2 cut(s) 434, 812
PspXI VCTCGAGB 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 32
PsuI RGATCY 1 cut(s) 834
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 5 cut(s) 90, 296, 611, 867, 1088
RsaNI GTAC 5 cut(s) 89, 295, 610, 866, 1087
RseI CAYNNNNRTG 1 cut(s) 118
SacI GAGCTC 1 cut(s) 148
SaqAI TTAA 5 cut(s) 584, 659, 798, 1035, 1150
SatI GCNGC 3 cut(s) 6, 54, 1027
Sau3AI GATC 7 cut(s) 151, 159, 172, 742, 781, 804, 834
Sau96I GGNCC 2 cut(s) 434, 812
SchI GAGTC 4 cut(s) 234, 256, 485, 1042
ScrFI CCNGG 3 cut(s) 369, 576, 1031
SduI GDGCHC 1 cut(s) 148
SfaNI GCATC 2 cut(s) 346, 715
Sfr274I CTCGAG 2 cut(s) 141, 1050
SinI GGWCC 1 cut(s) 812
SlaI CTCGAG 2 cut(s) 141, 1050
SmiI ATTTAAAT 1 cut(s) 660
SmiMI CAYNNNNRTG 1 cut(s) 118
SmlI CTYRAG 3 cut(s) 141, 530, 1050
SmoI CTYRAG 3 cut(s) 141, 530, 1050
Sse9I AATT 6 cut(s) 299, 613, 790, 948, 988, 997
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 1 cut(s) 482
SspMI CTAG 2 cut(s) 600, 734
SstI GAGCTC 1 cut(s) 148
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 3 cut(s) 367, 574, 1029
StyI CCWWGG 2 cut(s) 375, 918
SwaI ATTTAAAT 1 cut(s) 660
TaaI ACNGT 3 cut(s) 812, 1081, 1096
TaqI TCGA 4 cut(s) 142, 460, 493, 1051
TasI AATT 6 cut(s) 299, 613, 790, 948, 988, 997
TatI WGTACW 2 cut(s) 609, 865
TfiI GAWTC 1 cut(s) 1100
Tru1I TTAA 5 cut(s) 584, 659, 798, 1035, 1150
Tru9I TTAA 5 cut(s) 584, 659, 798, 1035, 1150
TscAI CASTG 2 cut(s) 432, 1101
TseFI GTSAC 5 cut(s) 259, 277, 320, 423, 760
TseI GCWGC 3 cut(s) 5, 53, 1026
Tsp45I GTSAC 5 cut(s) 259, 277, 320, 423, 760
TspDTI ATGAA 3 cut(s) 187, 425, 459
TspRI CASTG 2 cut(s) 432, 1101
VpaK11BI GGWCC 1 cut(s) 812
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 822
XhoI CTCGAG 2 cut(s) 141, 1050
XspI CTAG 2 cut(s) 600, 734
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.