FvH4_3g38520

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015921
Physical Location & Seq
Reverse (-)
32873855 .. 32875220
1366 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g38520.t1

Sequence Viewer

Length: 1155 bp
ATGTCAATGGCAGCTGAAGAAACTAGAAAACCAGAGGCTACTGCCATTGATAATGTTGCTGCCATTTTTGGGGTAACTGGGTTAGTTGGTAAGGTACTTGCCAGAAAGCTAATATCAAAACCCAAATGGAAGGTTTACGGTATAGCTCGAGCTCCTGATCATCAGATCATACCCGTTGATCAAAGTTGTTCAAACTTCCATTTCATCCAGTGTGATTTGCTAAACCCTTTGGAAACCCAGAAAAGACTCTCCTTGTTACAAGATGTGACTCATGTGTTTTGGGTCACTTGGGCAAGCCAGTACCAATTGGATAGCATTGAGTGCTGTGACCAAAACAAGGCCATGCTGTCCAACGCCTTGGATGCCATTATTCCTGGTGCCATGGCATTGAAACATGTTACTCTTCAGACTGGGATGAAGCATTATGTGTCGCTGCAAGGCCCTTTCGATGAAAATGAAGCTCGATACTACAATGAGGAGTCTCCGCGAGTGAAACCAGGCTGCAACTTCTATTATGTTCTTGAAGACTTGCTCAAGGAGAGGCTACATGGTAAGACAGCTTGGTCTGTGCATAGGCCTGGTTTGCTAATTGGTAGTTCTTCTAGGACATTGTACAATTTTATGGGTAGCTTATGCGTTTATGGGACTATTTGTAAGCATTTAAATCTCCCTTTTATGTTTGGAGGGACTAAAAAGTGTTGGGAAGAGACTTGTATTGATGGCTCAGATGCAAGGCTAGTAGCTGATCAGCAAATTTGGGCAGCCACCACTGCTGATTTGGATGAAAATGGCCATTCGTTTAATGCAATCAACGGTCCAAGTTTTACATGGAAGGAGATCTGGAAAGCTGTTGCAGGAAAGCTTGGAGTACAAGTATCAGAAGAACACACATTTTCTGAGGACTTCTGGTATTCAAAAGCCATGGCTGACAAGCAACAAGTCTGGAAACAAATTGTAGCAGAAAAAGGGCTGATTGAAACTGAGATGGACTATTTGGCCAATTGGGATTTCTTGGATGTTCTGTTTCGCTGCCCGGTTAAGATTTTGGGGACTCGAGACAAAGTTGATGGACTTGGTTTTGATGTAAGGTACCAGACAGTGGATTCCATCATGTATTGGATAGACTGTATGAGAGATGAGAAGCTAATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

385

Amino Acids

43.56

Weight (kDa)

5.73

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 79 - 331 3e-14 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58750 AT5G58750
fragaria_vesca FvH4_3g38520
malus_domestica MD03G1071000.v1.1 MD11G1075000.v1.1
prunus_persica Prupe.6G057800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0069421
rosa_laevigata RLG00000036051
rosa_multiflora Rmu_sc0002658.1_g000023
rosa_roxburghii Rroxscaffold_1G00011540
rosa_rugosa Rorug05G0397500
rosa_samantha Rh5BG473500 Rh5CG496000 Rh5DG485500
rosa_wichuraiana Rw5G042410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 562
Acc65I GGTACC 1 cut(s) 1089
AccB1I GGYRCC 2 cut(s) 377, 1089
AccB7I CCANNNNNTGG 1 cut(s) 1099
AccII CGCG 1 cut(s) 487
AciI CCGC 1 cut(s) 485
AcoI YGGCCR 2 cut(s) 790, 996
AcsI RAATTY 1 cut(s) 753
AcuI CTGAAG 2 cut(s) 36, 389
AfaI GTAC 5 cut(s) 96, 302, 614, 870, 1091
AfiI CCNNNNNNNGG 3 cut(s) 69, 337, 1099
AflIII ACRYGT 1 cut(s) 394
AgsI TTSAA 5 cut(s) 192, 391, 524, 915, 977
AjnI CCWGG 3 cut(s) 373, 496, 577
AjuI GAANNNNNNNTTGG 2 cut(s) 992, 1024
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 3 cut(s) 486, 701, 1050
Ama87I CYCGRG 2 cut(s) 147, 1053
AoxI GGCC 5 cut(s) 339, 439, 575, 790, 996
ApeKI GCWGC 6 cut(s) 11, 59, 433, 501, 761, 1029
ApoI RAATTY 1 cut(s) 753
Asp718I GGTACC 1 cut(s) 1089
AspS9I GGNCC 2 cut(s) 440, 815
AsuC2I CCSGG 1 cut(s) 1034
AvaI CYCGRG 2 cut(s) 147, 1053
AvaII GGWCC 1 cut(s) 815
BalI TGGCCA 2 cut(s) 792, 998
BanI GGYRCC 2 cut(s) 377, 1089
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 6 cut(s) 23, 46, 420, 488, 773, 1016
BccI CCATC 4 cut(s) 713, 979, 1061, 1115
BciT130I CCWGG 3 cut(s) 375, 498, 579
BclI TGATCA 3 cut(s) 157, 178, 745
BcnI CCSGG 1 cut(s) 1034
BcoDI GTCTC 3 cut(s) 486, 701, 1050
BfaI CTAG 3 cut(s) 24, 603, 737
BglII AGATCT 1 cut(s) 837
BisI GCNGC 6 cut(s) 12, 60, 434, 502, 762, 1030
BlsI GCNGC 6 cut(s) 13, 61, 435, 503, 763, 1031
Bme1390I CCNGG 4 cut(s) 375, 498, 579, 1034
Bme18I GGWCC 1 cut(s) 815
BmeT110I CYCGRG 2 cut(s) 147, 1053
BmgT120I GGNCC 2 cut(s) 440, 815
BmiI GGNNCC 2 cut(s) 379, 1091
BmrFI CCNGG 4 cut(s) 375, 498, 579, 1034
BmrI ACTGGG 2 cut(s) 87, 420
BmsI GCATC 2 cut(s) 352, 718
BmuI ACTGGG 2 cut(s) 87, 420
BpiI GAAGAC 1 cut(s) 531
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 1 cut(s) 1034
BsaJI CCNNGG 3 cut(s) 357, 381, 921
BsaXI ACNNNNNCTCC 2 cut(s) 858, 888
Bsc4I CCNNNNNNNGG 3 cut(s) 69, 337, 1099
Bse1I ACTGG 4 cut(s) 82, 208, 298, 415
BseBI CCWGG 3 cut(s) 375, 498, 579
BseDI CCNNGG 3 cut(s) 357, 381, 921
BseGI GGATG 5 cut(s) 204, 367, 420, 787, 1021
BseLI CCNNNNNNNGG 3 cut(s) 69, 337, 1099
BseMII CTCAG 3 cut(s) 738, 888, 972
BseNI ACTGG 4 cut(s) 82, 208, 298, 415
BseRI GAGGAG 1 cut(s) 491
BseXI GCAGC 6 cut(s) 23, 46, 420, 488, 773, 1016
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 5 cut(s) 341, 441, 577, 792, 998
BshNI GGYRCC 2 cut(s) 377, 1089
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 2 cut(s) 147, 1053
BsiSI CCGG 1 cut(s) 1034
BslFI GGGAC 3 cut(s) 658, 700, 1063
BslI CCNNNNNNNGG 3 cut(s) 69, 337, 1099
BsmAI GTCTC 3 cut(s) 486, 701, 1050
BsmFI GGGAC 3 cut(s) 658, 700, 1063
BsnI GGCC 5 cut(s) 341, 441, 577, 792, 998
BsoBI CYCGRG 2 cut(s) 147, 1053
Bsp1286I GDGCHC 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 612
Bsp143I GATC 5 cut(s) 157, 165, 178, 745, 837
Bsp19I CCATGG 2 cut(s) 381, 921
BspACI CCGC 1 cut(s) 485
BspANI GGCC 5 cut(s) 341, 441, 577, 792, 998
BspCNI CTCAG 3 cut(s) 737, 889, 973
BspFNI CGCG 1 cut(s) 487
BspLI GGNNCC 2 cut(s) 379, 1091
BspT107I GGYRCC 2 cut(s) 377, 1089
BsrGI TGTACA 1 cut(s) 612
BsrI ACTGG 4 cut(s) 82, 208, 298, 415
BssECI CCNNGG 3 cut(s) 357, 381, 921
BssMI GATC 5 cut(s) 157, 165, 178, 745, 837
BssT1I CCWWGG 3 cut(s) 357, 381, 921
Bst2UI CCWGG 3 cut(s) 375, 498, 579
Bst4CI ACNGT 4 cut(s) 140, 815, 1099, 1127
Bst6I CTCTTC 2 cut(s) 408, 699
BstAPI GCANNNNNTGC 1 cut(s) 321
BstAUI TGTACA 1 cut(s) 612
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 3 cut(s) 724, 897, 981
BstDSI CCRYGG 2 cut(s) 381, 921
BstF5I GGATG 5 cut(s) 204, 367, 420, 787, 1021
BstFNI CGCG 1 cut(s) 487
BstKTI GATC 5 cut(s) 160, 168, 181, 748, 840
BstMAI GTCTC 3 cut(s) 486, 701, 1050
BstMBI GATC 5 cut(s) 157, 165, 178, 745, 837
BstMWI GCNNNNNNNGC 4 cut(s) 321, 362, 583, 770
BstNI CCWGG 3 cut(s) 375, 498, 579
BstNSI RCATGY 1 cut(s) 398
BstSCI CCNGG 4 cut(s) 373, 496, 577, 1032
BstUI CGCG 1 cut(s) 487
BstV1I GCAGC 6 cut(s) 23, 46, 420, 488, 773, 1016
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 837
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 1 cut(s) 837
BsuRI GGCC 5 cut(s) 341, 441, 577, 792, 998
BtgI CCRYGG 2 cut(s) 381, 921
BtsCI GGATG 5 cut(s) 204, 367, 420, 787, 1021
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 3 cut(s) 215, 768, 1104
Cac8I GCNNGC 1 cut(s) 295
Cfr13I GGNCC 2 cut(s) 440, 815
Csp6I GTAC 5 cut(s) 95, 301, 613, 869, 1090
CviAII CATG 8 cut(s) 272, 343, 382, 395, 548, 828, 922, 1111
CviQI GTAC 5 cut(s) 95, 301, 613, 869, 1090
DdeI CTNAG 3 cut(s) 724, 897, 981
DpnI GATC 5 cut(s) 159, 167, 180, 747, 839
DpnII GATC 5 cut(s) 157, 165, 178, 745, 837
DraI TTTAAA 1 cut(s) 663
DrdI GACNNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 562
EaeI YGGCCR 2 cut(s) 790, 996
Eam1104I CTCTTC 2 cut(s) 408, 699
EarI CTCTTC 2 cut(s) 408, 699
Ecl136II GAGCTC 1 cut(s) 152
Eco130I CCWWGG 3 cut(s) 357, 381, 921
Eco147I AGGCCT 1 cut(s) 577
Eco24I GRGCYC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 815
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 2 cut(s) 36, 389
Eco88I CYCGRG 2 cut(s) 147, 1053
EcoICRI GAGCTC 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 3 cut(s) 373, 496, 577
EcoT14I CCWWGG 3 cut(s) 357, 381, 921
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 3 cut(s) 357, 381, 921
FaeI CATG 8 cut(s) 275, 346, 385, 398, 551, 831, 925, 1114
FaqI GGGAC 3 cut(s) 658, 700, 1063
FatI CATG 8 cut(s) 271, 342, 381, 394, 547, 827, 921, 1110
FbaI TGATCA 3 cut(s) 157, 178, 745
Fnu4HI GCNGC 6 cut(s) 12, 60, 434, 502, 762, 1030
FokI GGATG 5 cut(s) 191, 374, 427, 794, 1028
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 6 cut(s) 12, 60, 434, 502, 762, 1030
FspBI CTAG 3 cut(s) 24, 603, 737
GluI GCNGC 6 cut(s) 12, 60, 434, 502, 762, 1030
HaeIII GGCC 5 cut(s) 341, 441, 577, 792, 998
HapII CCGG 1 cut(s) 1034
Hin1II CATG 8 cut(s) 275, 346, 385, 398, 551, 831, 925, 1114
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 5 cut(s) 246, 268, 479, 1051, 1103
HpaII CCGG 1 cut(s) 1034
Hpy166II GTNNAC 1 cut(s) 136
Hpy188I TCNGA 5 cut(s) 165, 408, 727, 880, 898
Hpy188III TCNNGA 5 cut(s) 155, 521, 841, 943, 1055
Hpy8I GTNNAC 1 cut(s) 136
HpyAV CCTTC 2 cut(s) 124, 826
HpyCH4III ACNGT 4 cut(s) 140, 815, 1099, 1127
HpyCH4V TGCA 6 cut(s) 436, 504, 571, 731, 806, 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 321, 362, 583, 770
HpyF3I CTNAG 3 cut(s) 724, 897, 981
Hsp92II CATG 8 cut(s) 275, 346, 385, 398, 551, 831, 925, 1114
KpnI GGTACC 1 cut(s) 1093
Ksp22I TGATCA 3 cut(s) 157, 178, 745
Kzo9I GATC 5 cut(s) 157, 165, 178, 745, 837
LmnI GCTCC 1 cut(s) 157
Lsp1109I GCAGC 6 cut(s) 23, 46, 420, 488, 773, 1016
LweI GCATC 2 cut(s) 352, 718
MaeI CTAG 3 cut(s) 24, 603, 737
MaeIII GTNAC 6 cut(s) 73, 255, 265, 283, 326, 397
MalI GATC 5 cut(s) 159, 167, 180, 747, 839
MboI GATC 5 cut(s) 157, 165, 178, 745, 837
MboII GAAGA 6 cut(s) 29, 395, 536, 591, 716, 893
MfeI CAATTG 2 cut(s) 305, 1000
MflI RGATCY 1 cut(s) 837
MhlI GDGCHC 1 cut(s) 154
MlsI TGGCCA 2 cut(s) 792, 998
MluCI AATT 6 cut(s) 305, 588, 616, 753, 951, 1000
MluNI TGGCCA 2 cut(s) 792, 998
MlyI GAGTC 4 cut(s) 240, 262, 488, 1045
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 5 cut(s) 28, 469, 534, 677, 892
Mox20I TGGCCA 2 cut(s) 792, 998
MscI TGGCCA 2 cut(s) 792, 998
MseI TTAA 4 cut(s) 662, 801, 1038, 1153
Msp20I TGGCCA 2 cut(s) 792, 998
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 1 cut(s) 1034
MspR9I CCNGG 4 cut(s) 375, 498, 579, 1034
MunI CAATTG 2 cut(s) 305, 1000
MvaI CCWGG 3 cut(s) 375, 498, 579
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 4 cut(s) 321, 362, 583, 770
NciI CCSGG 1 cut(s) 1034
NcoI CCATGG 2 cut(s) 381, 921
NdeII GATC 5 cut(s) 157, 165, 178, 745, 837
NlaIII CATG 8 cut(s) 275, 346, 385, 398, 551, 831, 925, 1114
NlaIV GGNNCC 2 cut(s) 379, 1091
NmuCI GTSAC 3 cut(s) 265, 283, 326
NspI RCATGY 1 cut(s) 398
PaeR7I CTCGAG 2 cut(s) 147, 1053
PceI AGGCCT 1 cut(s) 577
PciI ACATGT 1 cut(s) 394
PfeI GAWTC 1 cut(s) 1103
PflMI CCANNNNNTGG 1 cut(s) 1099
PkrI GCNGC 6 cut(s) 13, 61, 435, 503, 763, 1031
PleI GAGTC 4 cut(s) 240, 262, 487, 1045
PpsI GAGTC 4 cut(s) 240, 262, 487, 1045
PscI ACATGT 1 cut(s) 394
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 3 cut(s) 373, 496, 577
PspGI CCWGG 3 cut(s) 373, 496, 577
PspN4I GGNNCC 2 cut(s) 379, 1091
PspPI GGNCC 2 cut(s) 440, 815
PspXI VCTCGAGB 1 cut(s) 147
PsuI RGATCY 1 cut(s) 837
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 5 cut(s) 96, 302, 614, 870, 1091
RsaNI GTAC 5 cut(s) 95, 301, 613, 869, 1090
SacI GAGCTC 1 cut(s) 154
SaqAI TTAA 4 cut(s) 662, 801, 1038, 1153
SatI GCNGC 6 cut(s) 12, 60, 434, 502, 762, 1030
Sau3AI GATC 5 cut(s) 157, 165, 178, 745, 837
Sau96I GGNCC 2 cut(s) 440, 815
SchI GAGTC 4 cut(s) 240, 262, 488, 1045
ScrFI CCNGG 4 cut(s) 375, 498, 579, 1034
SduI GDGCHC 1 cut(s) 154
SfaNI GCATC 2 cut(s) 352, 718
Sfr274I CTCGAG 2 cut(s) 147, 1053
SinI GGWCC 1 cut(s) 815
SlaI CTCGAG 2 cut(s) 147, 1053
SmiI ATTTAAAT 1 cut(s) 663
SmlI CTYRAG 3 cut(s) 147, 533, 1053
SmoI CTYRAG 3 cut(s) 147, 533, 1053
Sse9I AATT 6 cut(s) 305, 588, 616, 753, 951, 1000
SseBI AGGCCT 1 cut(s) 577
SsiI CCGC 1 cut(s) 485
SspMI CTAG 3 cut(s) 24, 603, 737
SstI GAGCTC 1 cut(s) 154
StuI AGGCCT 1 cut(s) 577
StyD4I CCNGG 4 cut(s) 373, 496, 577, 1032
StyI CCWWGG 3 cut(s) 357, 381, 921
SwaI ATTTAAAT 1 cut(s) 663
TaaI ACNGT 4 cut(s) 140, 815, 1099, 1127
TaqI TCGA 4 cut(s) 148, 447, 463, 1054
TasI AATT 6 cut(s) 305, 588, 616, 753, 951, 1000
TatI WGTACW 2 cut(s) 612, 868
TfiI GAWTC 1 cut(s) 1103
Tru1I TTAA 4 cut(s) 662, 801, 1038, 1153
Tru9I TTAA 4 cut(s) 662, 801, 1038, 1153
TscAI CASTG 3 cut(s) 215, 775, 1104
TseFI GTSAC 3 cut(s) 265, 283, 326
TseI GCWGC 6 cut(s) 11, 59, 433, 501, 761, 1029
Tsp45I GTSAC 3 cut(s) 265, 283, 326
TspDTI ATGAA 5 cut(s) 193, 431, 465, 471, 798
TspRI CASTG 3 cut(s) 215, 775, 1104
Van91I CCANNNNNTGG 1 cut(s) 1099
VpaK11BI GGWCC 1 cut(s) 815
XapI RAATTY 1 cut(s) 753
XceI RCATGY 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 2 cut(s) 775, 825
XhoI CTCGAG 2 cut(s) 147, 1053
XspI CTAG 3 cut(s) 24, 603, 737
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.