Rroxscaffold_1G00011540

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015921
Physical Location & Seq
Forward (+)
14572537 .. 14573694
1158 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00011540.1

Sequence Viewer

Length: 1158 bp
ATGTCAATGGCAGCTGAAGAAACTAAAAACCCAGAGGCTGCTGCCATTGATAATGTTGCAGCCATTTTTGGGGTAACTGGGTTAGTTGGGAAGGTACTTGCCAGAAAGCTAATATCAACACCCAAATGGAAGGTTTATGGTATAGCTCGAGCTCCTGATCATCAGATCATACCCATTGATCAAAGTTCTTCAAACTTCCATTTCATCCAATGTGATTTGCTAAACCCTTTGGAAACCCAGAAAAGACTCCCATTGTTACAAGATGTGACTCATGTCTTTTGGGTCACTTGGGCAGGCCAGTACCAATTGGATAGCATTGAGTGCTGTGACCAAAACAGAGCCATGATGTCCAATGCCTTGGATGCTATACTTCCTGGTGCCATGGCATTGAGGCATGTTTCTCTTCAGACTGGGATGAAGCATTATGTGTCACTGCAAGGCCCTTTTGATGAAACTGAAGAAGCTCGATACTACAATGAGGAGTCTCCGCGAGTAAATCCCAGCTACAACTTTTACTATGTTCTTGAAGACTTGCTCAAGGAGAGGCTACATGGTAAGATAGGTTGGTCTGTGCATAGGCCTGGTTTGTTAATCGGTAGTTCTTCTAGGACTTTGTACAATTTTATGGGTAGTTTATGTGTTTATGGGACTATTTGTAAGCATTTAAATCTCCCATTTGTGTTTGGAGGGACAAAAAAGTGTTGGGAAGAGACTTGTATTGATGGCTCAGATGCAAGGCTAGTAGCTGATCAACACATTTGGGCAGCCACAAATGTTGATTTGGATGATCATAGCAATTCGTTTAATGCGATCAACGGTCCAAGTTTTACATGGAAGGAGATCTGGAAATCTGTTGCAGAAAAGCTTGGAGTACAAGTATCAGAAGAACACACATTTTCTGAGGACTTCTGGTATTCAAAAGCCATGGCTGACAAGCAACAAGTGTGGGAACAAATTGTAGCAGAAAAAGGGCTGGTTGAAACTGAGATGGACAATTTGGCCAATTGGGATTTCTTGGATGTTCTGTTTCGCTGCCCGGTTAAGATTTTGGGGACTCGAGAAAAAGTTGATGGACTTGGTTTTACTGTAAGGTACAAGACAGTGGATTCAATCATGTATTGGATAGATTATATGAGAGATGAGAAACTAATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

385

Amino Acids

43.81

Weight (kDa)

5.34

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRISE PF22917 78 - 332 1.4e-13 PRISE-like Rossmann-fold domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58750 AT5G58750
fragaria_vesca FvH4_3g38520
malus_domestica MD03G1071000.v1.1 MD11G1075000.v1.1
prunus_persica Prupe.6G057800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0069421
rosa_laevigata RLG00000036051
rosa_multiflora Rmu_sc0002658.1_g000023
rosa_roxburghii Rroxscaffold_1G00011540
rosa_rugosa Rorug05G0397500
rosa_samantha Rh5BG473500 Rh5CG496000 Rh5DG485500
rosa_wichuraiana Rw5G042410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 377
AccII CGCG 1 cut(s) 490
AciI CCGC 1 cut(s) 488
AcoI YGGCCR 1 cut(s) 999
AcuI CTGAAG 3 cut(s) 36, 389, 477
AfaI GTAC 5 cut(s) 96, 302, 617, 873, 1094
AfiI CCNNNNNNNGG 1 cut(s) 69
AgsI TTSAA 5 cut(s) 192, 527, 918, 980, 1110
AjnI CCWGG 2 cut(s) 373, 580
AjuI GAANNNNNNNTTGG 2 cut(s) 995, 1027
AluBI AGCT 8 cut(s) 14, 109, 146, 152, 464, 504, 746, 865
AluI AGCT 8 cut(s) 14, 109, 146, 152, 464, 504, 746, 865
Alw21I GWGCWC 1 cut(s) 154
Alw26I GTCTC 2 cut(s) 489, 704
AlwNI CAGNNNCTG 1 cut(s) 38
Ama87I CYCGRG 2 cut(s) 147, 1056
AoxI GGCC 4 cut(s) 295, 439, 578, 999
ApeKI GCWGC 6 cut(s) 11, 38, 41, 59, 764, 1032
AspS9I GGNCC 2 cut(s) 440, 818
AsuC2I CCSGG 1 cut(s) 1037
AvaI CYCGRG 2 cut(s) 147, 1056
AvaII GGWCC 1 cut(s) 818
BalI TGGCCA 1 cut(s) 1001
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 534
Bbv12I GWGCWC 1 cut(s) 154
BbvI GCAGC 6 cut(s) 23, 25, 28, 71, 776, 1019
BccI CCATC 3 cut(s) 716, 982, 1064
BciT130I CCWGG 2 cut(s) 375, 582
BclI TGATCA 4 cut(s) 157, 178, 748, 787
BcnI CCSGG 1 cut(s) 1037
BcoDI GTCTC 2 cut(s) 489, 704
BfaI CTAG 2 cut(s) 606, 740
BglII AGATCT 1 cut(s) 840
BisI GCNGC 6 cut(s) 12, 39, 42, 60, 765, 1033
BlsI GCNGC 6 cut(s) 13, 40, 43, 61, 766, 1034
Bme1390I CCNGG 3 cut(s) 375, 582, 1037
Bme18I GGWCC 1 cut(s) 818
BmeT110I CYCGRG 2 cut(s) 147, 1056
BmgT120I GGNCC 2 cut(s) 440, 818
BmiI GGNNCC 1 cut(s) 379
BmrFI CCNGG 3 cut(s) 375, 582, 1037
BmrI ACTGGG 2 cut(s) 87, 420
BmsI GCATC 2 cut(s) 352, 721
BmuI ACTGGG 2 cut(s) 87, 420
BoxI GACNNNNGTC 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 521
BpuMI CCSGG 1 cut(s) 1037
BsaJI CCNNGG 3 cut(s) 357, 381, 924
BsaXI ACNNNNNCTCC 2 cut(s) 861, 891
Bsc4I CCNNNNNNNGG 1 cut(s) 69
Bse1I ACTGG 3 cut(s) 82, 298, 415
BseBI CCWGG 2 cut(s) 375, 582
BseDI CCNNGG 3 cut(s) 357, 381, 924
BseGI GGATG 5 cut(s) 204, 367, 420, 790, 1024
BseLI CCNNNNNNNGG 1 cut(s) 69
BseMII CTCAG 3 cut(s) 741, 891, 975
BseNI ACTGG 3 cut(s) 82, 298, 415
BseRI GAGGAG 1 cut(s) 494
BseXI GCAGC 6 cut(s) 23, 25, 28, 71, 776, 1019
BseYI CCCAGC 1 cut(s) 500
Bsh1236I CGCG 1 cut(s) 490
BshFI GGCC 4 cut(s) 297, 441, 580, 1001
BshNI GGYRCC 1 cut(s) 377
BsiHKAI GWGCWC 1 cut(s) 154
BsiHKCI CYCGRG 2 cut(s) 147, 1056
BsiSI CCGG 1 cut(s) 1037
BslFI GGGAC 3 cut(s) 661, 703, 1066
BslI CCNNNNNNNGG 1 cut(s) 69
BsmAI GTCTC 2 cut(s) 489, 704
BsmFI GGGAC 3 cut(s) 661, 703, 1066
BsnI GGCC 4 cut(s) 297, 441, 580, 1001
BsoBI CYCGRG 2 cut(s) 147, 1056
Bsp1286I GDGCHC 1 cut(s) 154
Bsp1407I TGTACA 1 cut(s) 615
Bsp143I GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
Bsp19I CCATGG 2 cut(s) 381, 924
BspACI CCGC 1 cut(s) 488
BspANI GGCC 4 cut(s) 297, 441, 580, 1001
BspCNI CTCAG 3 cut(s) 740, 892, 976
BspFNI CGCG 1 cut(s) 490
BspLI GGNNCC 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 377
BsrGI TGTACA 1 cut(s) 615
BsrI ACTGG 3 cut(s) 82, 298, 415
BssECI CCNNGG 3 cut(s) 357, 381, 924
BssMI GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
BssT1I CCWWGG 3 cut(s) 357, 381, 924
Bst2UI CCWGG 2 cut(s) 375, 582
Bst4CI ACNGT 3 cut(s) 818, 1087, 1102
Bst6I CTCTTC 2 cut(s) 408, 702
BstAPI GCANNNNNTGC 1 cut(s) 321
BstAUI TGTACA 1 cut(s) 615
BstC8I GCNNGC 1 cut(s) 295
BstDEI CTNAG 3 cut(s) 727, 900, 984
BstDSI CCRYGG 2 cut(s) 381, 924
BstF5I GGATG 5 cut(s) 204, 367, 420, 790, 1024
BstFNI CGCG 1 cut(s) 490
BstKTI GATC 7 cut(s) 160, 168, 181, 751, 790, 813, 843
BstMAI GTCTC 2 cut(s) 489, 704
BstMBI GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
BstMWI GCNNNNNNNGC 2 cut(s) 321, 362
BstNI CCWGG 2 cut(s) 375, 582
BstNSI RCATGY 1 cut(s) 398
BstPAI GACNNNNGTC 1 cut(s) 272
BstSCI CCNGG 3 cut(s) 373, 580, 1035
BstUI CGCG 1 cut(s) 490
BstV1I GCAGC 6 cut(s) 23, 25, 28, 71, 776, 1019
BstV2I GAAGAC 1 cut(s) 534
BstX2I RGATCY 1 cut(s) 840
BstXI CCANNNNNNTGG 1 cut(s) 358
BstYI RGATCY 1 cut(s) 840
BsuRI GGCC 4 cut(s) 297, 441, 580, 1001
BtgI CCRYGG 2 cut(s) 381, 924
BtsCI GGATG 5 cut(s) 204, 367, 420, 790, 1024
BtsI GCAGTG 1 cut(s) 431
BtsIMutI CAGTG 2 cut(s) 431, 1107
Cac8I GCNNGC 1 cut(s) 295
CaiI CAGNNNCTG 1 cut(s) 38
Cfr13I GGNCC 2 cut(s) 440, 818
Csp6I GTAC 5 cut(s) 95, 301, 616, 872, 1093
CspCI CAANNNNNGTGG 4 cut(s) 757, 792, 926, 961
CviAII CATG 8 cut(s) 272, 343, 382, 395, 551, 831, 925, 1114
CviQI GTAC 5 cut(s) 95, 301, 616, 872, 1093
DdeI CTNAG 3 cut(s) 727, 900, 984
DpnI GATC 7 cut(s) 159, 167, 180, 750, 789, 812, 842
DpnII GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
DraI TTTAAA 1 cut(s) 666
EaeI YGGCCR 1 cut(s) 999
Eam1104I CTCTTC 2 cut(s) 408, 702
EarI CTCTTC 2 cut(s) 408, 702
Ecl136II GAGCTC 1 cut(s) 152
Eco130I CCWWGG 3 cut(s) 357, 381, 924
Eco147I AGGCCT 1 cut(s) 580
Eco24I GRGCYC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 818
Eco53kI GAGCTC 1 cut(s) 152
Eco57I CTGAAG 3 cut(s) 36, 389, 477
Eco88I CYCGRG 2 cut(s) 147, 1056
EcoICRI GAGCTC 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 373, 580
EcoT14I CCWWGG 3 cut(s) 357, 381, 924
EcoT38I GRGCYC 1 cut(s) 154
ErhI CCWWGG 3 cut(s) 357, 381, 924
FaeI CATG 8 cut(s) 275, 346, 385, 398, 554, 834, 928, 1117
FaqI GGGAC 3 cut(s) 661, 703, 1066
FatI CATG 8 cut(s) 271, 342, 381, 394, 550, 830, 924, 1113
FbaI TGATCA 4 cut(s) 157, 178, 748, 787
Fnu4HI GCNGC 6 cut(s) 12, 39, 42, 60, 765, 1033
FokI GGATG 5 cut(s) 191, 374, 427, 797, 1031
FriOI GRGCYC 1 cut(s) 154
Fsp4HI GCNGC 6 cut(s) 12, 39, 42, 60, 765, 1033
FspBI CTAG 2 cut(s) 606, 740
GluI GCNGC 6 cut(s) 12, 39, 42, 60, 765, 1033
GsaI CCCAGC 1 cut(s) 504
HaeIII GGCC 4 cut(s) 297, 441, 580, 1001
HapII CCGG 1 cut(s) 1037
Hin1II CATG 8 cut(s) 275, 346, 385, 398, 554, 834, 928, 1117
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 5 cut(s) 246, 268, 482, 1054, 1106
HpaII CCGG 1 cut(s) 1037
Hpy188I TCNGA 5 cut(s) 165, 408, 730, 883, 901
Hpy188III TCNNGA 4 cut(s) 155, 524, 844, 1058
HpyAV CCTTC 3 cut(s) 85, 124, 829
HpyCH4III ACNGT 3 cut(s) 818, 1087, 1102
HpyCH4V TGCA 5 cut(s) 59, 436, 574, 734, 857
HpyF10VI GCNNNNNNNGC 2 cut(s) 321, 362
HpyF3I CTNAG 3 cut(s) 727, 900, 984
Hsp92II CATG 8 cut(s) 275, 346, 385, 398, 554, 834, 928, 1117
Ksp22I TGATCA 4 cut(s) 157, 178, 748, 787
Kzo9I GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
LmnI GCTCC 1 cut(s) 157
Lsp1109I GCAGC 6 cut(s) 23, 25, 28, 71, 776, 1019
LweI GCATC 2 cut(s) 352, 721
MaeI CTAG 2 cut(s) 606, 740
MaeIII GTNAC 6 cut(s) 73, 255, 265, 283, 326, 429
MalI GATC 7 cut(s) 159, 167, 180, 750, 789, 812, 842
MboI GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
MboII GAAGA 8 cut(s) 29, 180, 395, 470, 539, 594, 719, 896
MfeI CAATTG 2 cut(s) 305, 1003
MflI RGATCY 1 cut(s) 840
MhlI GDGCHC 1 cut(s) 154
MlsI TGGCCA 1 cut(s) 1001
MluCI AATT 6 cut(s) 305, 619, 796, 954, 994, 1003
MluNI TGGCCA 1 cut(s) 1001
MlyI GAGTC 4 cut(s) 240, 262, 491, 1048
MnlI CCTC 6 cut(s) 28, 384, 472, 537, 680, 895
Mox20I TGGCCA 1 cut(s) 1001
MscI TGGCCA 1 cut(s) 1001
MseI TTAA 5 cut(s) 590, 665, 804, 1041, 1156
MslI CAYNNNNRTG 1 cut(s) 124
Msp20I TGGCCA 1 cut(s) 1001
MspA1I CMGCKG 1 cut(s) 14
MspI CCGG 1 cut(s) 1037
MspR9I CCNGG 3 cut(s) 375, 582, 1037
MunI CAATTG 2 cut(s) 305, 1003
MvaI CCWGG 2 cut(s) 375, 582
MvnI CGCG 1 cut(s) 490
MwoI GCNNNNNNNGC 2 cut(s) 321, 362
NciI CCSGG 1 cut(s) 1037
NcoI CCATGG 2 cut(s) 381, 924
NdeII GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
NlaIII CATG 8 cut(s) 275, 346, 385, 398, 554, 834, 928, 1117
NlaIV GGNNCC 1 cut(s) 379
NmuCI GTSAC 4 cut(s) 265, 283, 326, 429
NspI RCATGY 1 cut(s) 398
PaeR7I CTCGAG 2 cut(s) 147, 1056
PceI AGGCCT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 806
PfeI GAWTC 1 cut(s) 1106
PkrI GCNGC 6 cut(s) 13, 40, 43, 61, 766, 1034
PleI GAGTC 4 cut(s) 240, 262, 490, 1048
PpsI GAGTC 4 cut(s) 240, 262, 490, 1048
PshAI GACNNNNGTC 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 154
Psp6I CCWGG 2 cut(s) 373, 580
PspFI CCCAGC 1 cut(s) 500
PspGI CCWGG 2 cut(s) 373, 580
PspN4I GGNNCC 1 cut(s) 379
PspPI GGNCC 2 cut(s) 440, 818
PspXI VCTCGAGB 1 cut(s) 147
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 1 cut(s) 840
PvuII CAGCTG 1 cut(s) 14
RsaI GTAC 5 cut(s) 96, 302, 617, 873, 1094
RsaNI GTAC 5 cut(s) 95, 301, 616, 872, 1093
RseI CAYNNNNRTG 1 cut(s) 124
SacI GAGCTC 1 cut(s) 154
SaqAI TTAA 5 cut(s) 590, 665, 804, 1041, 1156
SatI GCNGC 6 cut(s) 12, 39, 42, 60, 765, 1033
Sau3AI GATC 7 cut(s) 157, 165, 178, 748, 787, 810, 840
Sau96I GGNCC 2 cut(s) 440, 818
SchI GAGTC 4 cut(s) 240, 262, 491, 1048
ScrFI CCNGG 3 cut(s) 375, 582, 1037
SduI GDGCHC 1 cut(s) 154
SfaNI GCATC 2 cut(s) 352, 721
Sfr274I CTCGAG 2 cut(s) 147, 1056
SinI GGWCC 1 cut(s) 818
SlaI CTCGAG 2 cut(s) 147, 1056
SmiI ATTTAAAT 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 124
SmlI CTYRAG 3 cut(s) 147, 536, 1056
SmoI CTYRAG 3 cut(s) 147, 536, 1056
Sse9I AATT 6 cut(s) 305, 619, 796, 954, 994, 1003
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 1 cut(s) 488
SspMI CTAG 2 cut(s) 606, 740
SstI GAGCTC 1 cut(s) 154
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 3 cut(s) 373, 580, 1035
StyI CCWWGG 3 cut(s) 357, 381, 924
SwaI ATTTAAAT 1 cut(s) 666
TaaI ACNGT 3 cut(s) 818, 1087, 1102
TaqI TCGA 3 cut(s) 148, 466, 1057
TasI AATT 6 cut(s) 305, 619, 796, 954, 994, 1003
TatI WGTACW 2 cut(s) 615, 871
TfiI GAWTC 1 cut(s) 1106
Tru1I TTAA 5 cut(s) 590, 665, 804, 1041, 1156
Tru9I TTAA 5 cut(s) 590, 665, 804, 1041, 1156
TscAI CASTG 2 cut(s) 438, 1107
TseFI GTSAC 4 cut(s) 265, 283, 326, 429
TseI GCWGC 6 cut(s) 11, 38, 41, 59, 764, 1032
Tsp45I GTSAC 4 cut(s) 265, 283, 326, 429
TspDTI ATGAA 3 cut(s) 193, 431, 465
TspRI CASTG 2 cut(s) 438, 1107
VpaK11BI GGWCC 1 cut(s) 818
XceI RCATGY 1 cut(s) 398
XcmI CCANNNNNNNNNTGG 1 cut(s) 828
XhoI CTCGAG 2 cut(s) 147, 1056
XspI CTAG 2 cut(s) 606, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.