MD03G1071000.v1.1

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015921
Physical Location & Seq
Forward (+)
5794586 .. 5795719
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1071000.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGCAGCTGAAGAAACTAAAAATCCAGAGGTTGGGATTGAACATGTAGTAGCGATTTTCGGGGTCACAGGGCTAGTTGGGAAGGTTCTGGCCAGAGAGCTGGTCACAAAACCCAAATGGAAGGTTTACGGCATAGCTCGAAAGCTAGAGATCATGCTACCCATTGATCAATGTTCATCGAACTTCCATTTCATCCAATGTGATCTGCTAAACCCTTCGGAAACCAAAGATCGGCTTTCATTGTTGCAAAATGTTACTCTCGTGTTTTGGCTCACTTGGCCAAGCCAATGCCAGTTGGATAGCATTGAGTGTTGCGAGCAGAATAAGACTATGATGTCGAATGCATTGGATTCCATTGTTCTGAAAACAAAGGCATTGCAGCATGTTTCTCTCCAGACAGGGATGAAGCATTACGTGTCATTGCAAGGGCCTTTTGATGATGAAGTAGAAGCTCGATACTACAATGAAGATTGCCCGAGAGTGGATAAAGGGTGCAATTTTTACTATGTCCTAGAAGACTTGCTTAAGGAAGGCTGGCCGACTTGTATCGATGGCTCGGATGCAAGGCTAGTAGCGCAACGGTATATTTGGGCAGCAACCAATGATGGTTTGTACTCATATTCCACTGACGGTCAGTCATACAATGCAATCAATGGGCCAAGTTTTACATGGAAGGAAATCTGGACGGCTCTTGGAGAGAAATTCGGAGTAGAAGTAATACAGGAAAGTGCATTTTCGGATGAGTTCTGGTATTCGAAATCCTTGGCAGACAAGAAAGAGGTTTGGAAAGAAATTGTAGCAGAGAAAGGACTGGTTCAAACTGAGATGGAAAATTTGGCCAATTGGGAATTCTTGGACGTCTTGTTTCGTTGTCCGTTTAAGCTATTGGGAACCCAAAACAAAGTTGATCGACTACGGTTTACTGTAAGGTATAAGACACTGGATTCAATCTTGTACTGGATAGATTGTATGGGATATGAAAAATTAATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

332

Amino Acids

38.05

Weight (kDa)

5.03

Isoelectric Point (pI)

27.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58750 AT5G58750
fragaria_vesca FvH4_3g38520
malus_domestica MD03G1071000.v1.1 MD11G1075000.v1.1
prunus_persica Prupe.6G057800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0069421
rosa_laevigata RLG00000036051
rosa_multiflora Rmu_sc0002658.1_g000023
rosa_roxburghii Rroxscaffold_1G00011540
rosa_rugosa Rorug05G0397500
rosa_samantha Rh5BG473500 Rh5CG496000 Rh5DG485500
rosa_wichuraiana Rw5G042410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 334
AatII GACGTC 1 cut(s) 861
AccB7I CCANNNNNTGG 1 cut(s) 32
AcoI YGGCCR 4 cut(s) 90, 278, 536, 837
AcsI RAATTY 3 cut(s) 701, 832, 848
AcuI CTGAAG 1 cut(s) 30
AcyI GRCGYC 1 cut(s) 858
AfaI GTAC 2 cut(s) 614, 956
AfiI CCNNNNNNNGG 3 cut(s) 32, 231, 481
AflII CTTAAG 1 cut(s) 524
AflIII ACRYGT 2 cut(s) 43, 414
AgsI TTSAA 3 cut(s) 41, 818, 948
AhdI GACNNNNNGTC 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
AluBI AGCT 6 cut(s) 8, 100, 137, 145, 452, 883
AluI AGCT 6 cut(s) 8, 100, 137, 145, 452, 883
Ama87I CYCGRG 1 cut(s) 475
AoxI GGCC 6 cut(s) 90, 278, 428, 536, 656, 837
ApeKI GCWGC 3 cut(s) 5, 379, 593
ApoI RAATTY 3 cut(s) 701, 832, 848
AseI ATTAAT 1 cut(s) 986
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 2 cut(s) 428, 656
AsuII TTCGAA 1 cut(s) 755
AvaI CYCGRG 1 cut(s) 475
BalI TGGCCA 3 cut(s) 92, 280, 839
BauI CACGAG 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 522
BbvI GCAGC 3 cut(s) 17, 391, 605
BccI CCATC 3 cut(s) 545, 599, 820
BceAI ACGGC 2 cut(s) 145, 702
BclI TGATCA 1 cut(s) 166
BfaI CTAG 4 cut(s) 74, 146, 512, 569
BfrI CTTAAG 1 cut(s) 524
BisI GCNGC 3 cut(s) 6, 380, 594
BlsI GCNGC 3 cut(s) 7, 381, 595
BmeRI GACNNNNNGTC 1 cut(s) 634
BmeT110I CYCGRG 1 cut(s) 475
BmgT120I GGNCC 2 cut(s) 428, 656
BmiI GGNNCC 1 cut(s) 892
BmsI GCATC 1 cut(s) 550
BpiI GAAGAC 1 cut(s) 522
BpmI CTGGAG 1 cut(s) 377
Bpu14I TTCGAA 1 cut(s) 755
Bsa29I ATCGAT 1 cut(s) 549
BsaAI YACGTR 1 cut(s) 415
BsaHI GRCGYC 1 cut(s) 858
BsaJI CCNNGG 1 cut(s) 762
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 231, 481
Bse1I ACTGG 4 cut(s) 292, 816, 945, 962
Bse3DI GCAATG 2 cut(s) 374, 419
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 4 cut(s) 192, 408, 565, 745
BseLI CCNNNNNNNGG 3 cut(s) 32, 231, 481
BseMI GCAATG 2 cut(s) 374, 419
BseMII CTCAG 1 cut(s) 813
BseNI ACTGG 4 cut(s) 292, 816, 945, 962
BseXI GCAGC 3 cut(s) 17, 391, 605
BshFI GGCC 6 cut(s) 92, 280, 430, 538, 658, 839
BshVI ATCGAT 1 cut(s) 549
BsiHKCI CYCGRG 1 cut(s) 475
BslI CCNNNNNNNGG 3 cut(s) 32, 231, 481
BsmI GAATGC 1 cut(s) 346
BsnI GGCC 6 cut(s) 92, 280, 430, 538, 658, 839
BsoBI CYCGRG 1 cut(s) 475
Bsp119I TTCGAA 1 cut(s) 755
Bsp143I GATC 5 cut(s) 150, 166, 202, 229, 907
BspANI GGCC 6 cut(s) 92, 280, 430, 538, 658, 839
BspCNI CTCAG 1 cut(s) 814
BspDI ATCGAT 1 cut(s) 549
BspLI GGNNCC 1 cut(s) 892
BspT104I TTCGAA 1 cut(s) 755
BspTI CTTAAG 1 cut(s) 524
BsrDI GCAATG 2 cut(s) 374, 419
BsrI ACTGG 4 cut(s) 292, 816, 945, 962
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 5 cut(s) 150, 166, 202, 229, 907
BssNI GRCGYC 1 cut(s) 858
BssSI CACGAG 1 cut(s) 260
BssT1I CCWWGG 1 cut(s) 762
Bst2BI CACGAG 1 cut(s) 260
Bst4CI ACNGT 4 cut(s) 582, 632, 918, 925
BstACI GRCGYC 1 cut(s) 858
BstAFI CTTAAG 1 cut(s) 524
BstBAI YACGTR 1 cut(s) 415
BstBI TTCGAA 1 cut(s) 755
BstC8I GCNNGC 2 cut(s) 317, 536
BstDEI CTNAG 1 cut(s) 822
BstF5I GGATG 4 cut(s) 192, 408, 565, 745
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 5 cut(s) 153, 169, 205, 232, 910
BstMBI GATC 5 cut(s) 150, 166, 202, 229, 907
BstMWI GCNNNNNNNGC 2 cut(s) 277, 574
BstNSI RCATGY 2 cut(s) 47, 386
BstV1I GCAGC 3 cut(s) 17, 391, 605
BstV2I GAAGAC 1 cut(s) 522
BstXI CCANNNNNNTGG 1 cut(s) 100
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 6 cut(s) 92, 280, 430, 538, 658, 839
BsuTUI ATCGAT 1 cut(s) 549
BtsCI GGATG 4 cut(s) 192, 408, 565, 745
BtsIMutI CAGTG 2 cut(s) 624, 938
Cac8I GCNNGC 2 cut(s) 317, 536
CfoI GCGC 1 cut(s) 577
Cfr13I GGNCC 2 cut(s) 428, 656
ClaI ATCGAT 1 cut(s) 549
Csp6I GTAC 2 cut(s) 613, 955
CviAII CATG 4 cut(s) 44, 154, 383, 669
CviQI GTAC 2 cut(s) 613, 955
DdeI CTNAG 1 cut(s) 822
DpnI GATC 5 cut(s) 152, 168, 204, 231, 909
DpnII GATC 5 cut(s) 150, 166, 202, 229, 907
DrdI GACNNNNNNGTC 1 cut(s) 334
DriI GACNNNNNGTC 1 cut(s) 634
DseDI GACNNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 4 cut(s) 90, 278, 536, 837
Eam1105I GACNNNNNGTC 1 cut(s) 634
Eco130I CCWWGG 1 cut(s) 762
Eco57I CTGAAG 1 cut(s) 30
Eco88I CYCGRG 1 cut(s) 475
EcoO109I RGGNCCY 1 cut(s) 428
EcoRI GAATTC 1 cut(s) 848
EcoT14I CCWWGG 1 cut(s) 762
EcoT22I ATGCAT 1 cut(s) 346
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 4 cut(s) 47, 157, 386, 672
FalI AAGNNNNNCTT 4 cut(s) 219, 251, 507, 539
FatI CATG 4 cut(s) 43, 153, 382, 668
FbaI TGATCA 1 cut(s) 166
Fnu4HI GCNGC 3 cut(s) 6, 380, 594
FokI GGATG 4 cut(s) 179, 415, 572, 752
Fsp4HI GCNGC 3 cut(s) 6, 380, 594
FspBI CTAG 4 cut(s) 74, 146, 512, 569
GlaI GCGC 1 cut(s) 576
GluI GCNGC 3 cut(s) 6, 380, 594
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 6 cut(s) 92, 280, 430, 538, 658, 839
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 858
Hin1II CATG 4 cut(s) 47, 157, 386, 672
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HinfI GANTC 2 cut(s) 350, 944
Hpy166II GTNNAC 2 cut(s) 127, 921
Hpy188I TCNGA 5 cut(s) 220, 363, 559, 707, 739
Hpy188III TCNNGA 3 cut(s) 26, 394, 682
Hpy8I GTNNAC 2 cut(s) 127, 921
HpyAV CCTTC 5 cut(s) 76, 115, 225, 524, 667
HpyCH4III ACNGT 4 cut(s) 582, 632, 918, 925
HpyCH4IV ACGT 2 cut(s) 414, 858
HpyCH4V TGCA 8 cut(s) 247, 344, 379, 424, 495, 563, 647, 731
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 574
HpyF3I CTNAG 1 cut(s) 822
HpySE526I ACGT 2 cut(s) 414, 858
Hsp92I GRCGYC 1 cut(s) 858
Hsp92II CATG 4 cut(s) 47, 157, 386, 672
HspAI GCGC 1 cut(s) 575
Ksp22I TGATCA 1 cut(s) 166
Kzo9I GATC 5 cut(s) 150, 166, 202, 229, 907
Lsp1109I GCAGC 3 cut(s) 17, 391, 605
LweI GCATC 1 cut(s) 550
MaeI CTAG 4 cut(s) 74, 146, 512, 569
MaeII ACGT 2 cut(s) 414, 858
MaeIII GTNAC 3 cut(s) 64, 103, 253
MalI GATC 5 cut(s) 152, 168, 204, 231, 909
MboI GATC 5 cut(s) 150, 166, 202, 229, 907
MboII GAAGA 3 cut(s) 23, 479, 527
MfeI CAATTG 1 cut(s) 841
MlsI TGGCCA 3 cut(s) 92, 280, 839
MluCI AATT 7 cut(s) 496, 701, 792, 832, 841, 848, 983
MluNI TGGCCA 3 cut(s) 92, 280, 839
MmeI TCCRAC 1 cut(s) 276
MnlI CCTC 2 cut(s) 22, 772
Mox20I TGGCCA 3 cut(s) 92, 280, 839
Mph1103I ATGCAT 1 cut(s) 346
MscI TGGCCA 3 cut(s) 92, 280, 839
MseI TTAA 4 cut(s) 525, 879, 986, 994
Msp20I TGGCCA 3 cut(s) 92, 280, 839
MspA1I CMGCKG 1 cut(s) 8
MspCI CTTAAG 1 cut(s) 524
MunI CAATTG 1 cut(s) 841
Mva1269I GAATGC 1 cut(s) 346
MwoI GCNNNNNNNGC 2 cut(s) 277, 574
NdeII GATC 5 cut(s) 150, 166, 202, 229, 907
NlaIII CATG 4 cut(s) 47, 157, 386, 672
NlaIV GGNNCC 1 cut(s) 892
NmuCI GTSAC 2 cut(s) 64, 103
NsiI ATGCAT 1 cut(s) 346
NspI RCATGY 2 cut(s) 47, 386
NspV TTCGAA 1 cut(s) 755
PciI ACATGT 1 cut(s) 43
PctI GAATGC 1 cut(s) 346
PfeI GAWTC 2 cut(s) 350, 944
PflMI CCANNNNNTGG 1 cut(s) 32
PkrI GCNGC 3 cut(s) 7, 381, 595
Ppu21I YACGTR 1 cut(s) 415
PscI ACATGT 1 cut(s) 43
PshBI ATTAAT 1 cut(s) 986
PspN4I GGNNCC 1 cut(s) 892
PspPI GGNCC 2 cut(s) 428, 656
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 2 cut(s) 614, 956
RsaNI GTAC 2 cut(s) 613, 955
SaqAI TTAA 4 cut(s) 525, 879, 986, 994
SatI GCNGC 3 cut(s) 6, 380, 594
Sau3AI GATC 5 cut(s) 150, 166, 202, 229, 907
Sau96I GGNCC 2 cut(s) 428, 656
SfaNI GCATC 1 cut(s) 550
SfuI TTCGAA 1 cut(s) 755
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 7 cut(s) 496, 701, 792, 832, 841, 848, 983
SspMI CTAG 4 cut(s) 74, 146, 512, 569
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 4 cut(s) 582, 632, 918, 925
TaiI ACGT 2 cut(s) 417, 861
TaqI TCGA 7 cut(s) 139, 179, 338, 454, 549, 755, 910
TasI AATT 7 cut(s) 496, 701, 792, 832, 841, 848, 983
TatI WGTACW 2 cut(s) 612, 954
TfiI GAWTC 2 cut(s) 350, 944
Tru1I TTAA 4 cut(s) 525, 879, 986, 994
Tru9I TTAA 4 cut(s) 525, 879, 986, 994
TscAI CASTG 2 cut(s) 631, 945
TseFI GTSAC 2 cut(s) 64, 103
TseI GCWGC 3 cut(s) 5, 379, 593
Tsp45I GTSAC 2 cut(s) 64, 103
TspDTI ATGAA 7 cut(s) 165, 181, 228, 419, 456, 480, 993
TspGWI ACGGA 1 cut(s) 864
TspRI CASTG 2 cut(s) 631, 945
Van91I CCANNNNNTGG 1 cut(s) 32
Vha464I CTTAAG 1 cut(s) 524
VspI ATTAAT 1 cut(s) 986
XapI RAATTY 3 cut(s) 701, 832, 848
XceI RCATGY 2 cut(s) 47, 386
XcmI CCANNNNNNNNNTGG 1 cut(s) 666
XspI CTAG 4 cut(s) 74, 146, 512, 569
ZraI GACGTC 1 cut(s) 859
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.