Prupe.6G057800_v2.0.a1

3-oxo-Delta(4,5)-steroid 5-beta-reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0015921
Physical Location & Seq
Forward (+)
4038700 .. 4040262
1563 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G057800.1

Sequence Viewer

Length: 1014 bp
ATGGCAGCTGAAGAAACTAAAAGACCAGAGGCAGCTGGCATTGAACATGTTGCAGCCATTTTTGGTGTTACAGGGCTAGTTGGGAAGGTGCTGGCCAAAGAGCTAATCTCAAAATCCAAATGGAAGGTTTATGGTATAGCTCGAAATCCGATGATCATATCGAATCAGCAATGTTCAAATTTCCATTTCATCCAATGTGATTTGCTAAACCCTTTGGAAACCCAGCAAAGGCTCTCATTGTTACAAGATGTGACTCATGTGTTTTGGGTCACTTGGGCAAGCCAATACCAGTTAGATAGCATTGAGTGTTGTGAGCAAAACAAGGCCATGATGTCCAATGTCTTGAATGCCATCGTCCCCACGGCCAAGGCATTGAAGCATGTTTCTCTTCAGACAGGGATGAAGCATTATGTGTCATTGCAGGGGCCCTTCGATGAAGCAGAAGCTCGGTACTACAATGAGAATGGCCCGAGAGTGAAAGCAGGCTGCAATTTTTACTATGTGCTGGAAGACTTGCTCAAGGAGAGGCTGGATGGGACAAAGAAGTGTTGGGAAGAGACCTGCATTGACGGCTCAGATGCAAGACTAGTAGCTGAACAACACATTTGGGCAGCCACCAATGATGATGTAAATGTACAATCCACTGATGGCCAGTCATTCAATGCAATCAATGGTCCAAGTTTCACATGGAAGGAGATCTGGACAGCACTTGCAGAAAAGTTTGGACTAGAAGTAGTACCCGAAAATGCATTTTCGGAGGACTTCTGGTATTCGAAAGCAATGGCTGACAAGAAAGAAGTTTGGAAAGAAATTGTTGCAAAGGAAAGGATGATGAAAACTGAGATGGAAGATTTGGCCAATTGGGCATTCTTGGATGTCTTGTTTCGTTGTCCGTTTAAGCTATTGGGAACCCGAAACAAAGTTGATCAGCTTGGTTTTACTGTAAGGTATAAAACACTGGATTCAATCTTGTATTGGATAGATTGTATGAGAGATGAAAAATTAATACCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

338

Amino Acids

38.55

Weight (kDa)

5.43

Isoelectric Point (pI)

31.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015921)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G58750 AT5G58750
fragaria_vesca FvH4_3g38520
malus_domestica MD03G1071000.v1.1 MD11G1075000.v1.1
prunus_persica Prupe.6G057800_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0069421
rosa_laevigata RLG00000036051
rosa_multiflora Rmu_sc0002658.1_g000023
rosa_roxburghii Rroxscaffold_1G00011540
rosa_rugosa Rorug05G0397500
rosa_samantha Rh5BG473500 Rh5CG496000 Rh5DG485500
rosa_wichuraiana Rw5G042410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 569
AcoI YGGCCR 4 cut(s) 93, 363, 649, 855
AcsI RAATTY 1 cut(s) 178
AcuI CTGAAG 2 cut(s) 30, 374
AfaI GTAC 3 cut(s) 452, 636, 738
AfiI CCNNNNNNNGG 1 cut(s) 228
AflIII ACRYGT 1 cut(s) 46
AgsI TTSAA 6 cut(s) 44, 177, 346, 376, 661, 966
AhlI ACTAGT 1 cut(s) 586
AjuI GAANNNNNNNTTGG 2 cut(s) 851, 883
AluBI AGCT 8 cut(s) 8, 35, 103, 140, 446, 593, 901, 931
AluI AGCT 8 cut(s) 8, 35, 103, 140, 446, 593, 901, 931
Alw26I GTCTC 1 cut(s) 551
Ama87I CYCGRG 1 cut(s) 469
AoxI GGCC 7 cut(s) 93, 324, 363, 425, 466, 649, 855
ApaI GGGCCC 1 cut(s) 429
ApeKI GCWGC 5 cut(s) 5, 32, 53, 486, 611
ApoI RAATTY 1 cut(s) 178
AseI ATTAAT 1 cut(s) 1004
AspS9I GGNCC 4 cut(s) 425, 426, 467, 674
AsuII TTCGAA 1 cut(s) 773
AvaI CYCGRG 1 cut(s) 469
AvaII GGWCC 1 cut(s) 674
BaeGI GKGCMC 1 cut(s) 429
BalI TGGCCA 3 cut(s) 95, 651, 857
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 516
BbvI GCAGC 5 cut(s) 17, 44, 65, 473, 623
BccI CCATC 4 cut(s) 359, 527, 641, 838
BceAI ACGGC 2 cut(s) 378, 586
BclI TGATCA 2 cut(s) 153, 925
BcoDI GTCTC 1 cut(s) 551
BcuI ACTAGT 1 cut(s) 586
BfaI CTAG 3 cut(s) 77, 587, 728
BfuAI ACCTGC 1 cut(s) 569
BglI GCCNNNNNGGC 1 cut(s) 863
BglII AGATCT 1 cut(s) 696
BisI GCNGC 5 cut(s) 6, 33, 54, 487, 612
BlsI GCNGC 5 cut(s) 7, 34, 55, 488, 613
Bme18I GGWCC 1 cut(s) 674
BmeT110I CYCGRG 1 cut(s) 469
BmgT120I GGNCC 4 cut(s) 425, 426, 467, 674
BmiI GGNNCC 3 cut(s) 426, 427, 910
BmsI GCATC 1 cut(s) 568
BpiI GAAGAC 1 cut(s) 516
BplI GAGNNNNNCTC 2 cut(s) 92, 124
Bpu14I TTCGAA 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 503
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 2 cut(s) 360, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 228
Bse1I ACTGG 3 cut(s) 289, 652, 963
Bse3DI GCAATG 3 cut(s) 176, 416, 786
BseDI CCNNGG 2 cut(s) 360, 366
BseGI GGATG 5 cut(s) 189, 405, 538, 834, 880
BseLI CCNNNNNNNGG 1 cut(s) 228
BseMI GCAATG 3 cut(s) 176, 416, 786
BseMII CTCAG 2 cut(s) 588, 831
BseNI ACTGG 3 cut(s) 289, 652, 963
BseSI GKGCMC 1 cut(s) 429
BseXI GCAGC 5 cut(s) 17, 44, 65, 473, 623
BseYI CCCAGC 1 cut(s) 222
BshFI GGCC 7 cut(s) 95, 326, 365, 427, 468, 651, 857
BsiHKCI CYCGRG 1 cut(s) 469
BslFI GGGAC 2 cut(s) 341, 550
BslI CCNNNNNNNGG 1 cut(s) 228
BsmAI GTCTC 1 cut(s) 551
BsmFI GGGAC 2 cut(s) 341, 550
BsmI GAATGC 2 cut(s) 352, 866
BsnI GGCC 7 cut(s) 95, 326, 365, 427, 468, 651, 857
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 1 cut(s) 469
Bsp119I TTCGAA 1 cut(s) 773
Bsp120I GGGCCC 1 cut(s) 425
Bsp1286I GDGCHC 1 cut(s) 429
Bsp1407I TGTACA 1 cut(s) 634
Bsp143I GATC 3 cut(s) 153, 696, 925
BspANI GGCC 7 cut(s) 95, 326, 365, 427, 468, 651, 857
BspCNI CTCAG 2 cut(s) 587, 832
BspLI GGNNCC 3 cut(s) 426, 427, 910
BspMI ACCTGC 1 cut(s) 569
BspT104I TTCGAA 1 cut(s) 773
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 3 cut(s) 176, 416, 786
BsrGI TGTACA 1 cut(s) 634
BsrI ACTGG 3 cut(s) 289, 652, 963
BssECI CCNNGG 2 cut(s) 360, 366
BssMI GATC 3 cut(s) 153, 696, 925
BssT1I CCWWGG 1 cut(s) 366
Bst4CI ACNGT 1 cut(s) 943
Bst6I CTCTTC 2 cut(s) 393, 549
BstAUI TGTACA 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 773
BstC8I GCNNGC 4 cut(s) 37, 93, 280, 484
BstDEI CTNAG 2 cut(s) 574, 840
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 5 cut(s) 189, 405, 538, 834, 880
BstKTI GATC 3 cut(s) 156, 699, 928
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 3 cut(s) 153, 696, 925
BstMWI GCNNNNNNNGC 2 cut(s) 570, 863
BstNSI RCATGY 2 cut(s) 50, 383
BstSLI GKGCMC 1 cut(s) 429
BstV1I GCAGC 5 cut(s) 17, 44, 65, 473, 623
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 1 cut(s) 696
BstYI RGATCY 1 cut(s) 696
BsuRI GGCC 7 cut(s) 95, 326, 365, 427, 468, 651, 857
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 5 cut(s) 189, 405, 538, 834, 880
BtsIMutI CAGTG 2 cut(s) 642, 956
BveI ACCTGC 1 cut(s) 569
Cac8I GCNNGC 4 cut(s) 37, 93, 280, 484
Cfr13I GGNCC 4 cut(s) 425, 426, 467, 674
Csp6I GTAC 3 cut(s) 451, 635, 737
CviAII CATG 5 cut(s) 47, 257, 328, 380, 687
CviQI GTAC 3 cut(s) 451, 635, 737
DdeI CTNAG 2 cut(s) 574, 840
DpnI GATC 3 cut(s) 155, 698, 927
DpnII GATC 3 cut(s) 153, 696, 925
EaeI YGGCCR 4 cut(s) 93, 363, 649, 855
Eam1104I CTCTTC 2 cut(s) 393, 549
EarI CTCTTC 2 cut(s) 393, 549
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 551
Eco47I GGWCC 1 cut(s) 674
Eco57I CTGAAG 2 cut(s) 30, 374
Eco88I CYCGRG 1 cut(s) 469
EcoO109I RGGNCCY 2 cut(s) 425, 426
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 751
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 5 cut(s) 50, 260, 331, 383, 690
FaqI GGGAC 2 cut(s) 341, 550
FatI CATG 5 cut(s) 46, 256, 327, 379, 686
FbaI TGATCA 2 cut(s) 153, 925
Fnu4HI GCNGC 5 cut(s) 6, 33, 54, 487, 612
FokI GGATG 5 cut(s) 176, 412, 545, 841, 887
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 6, 33, 54, 487, 612
FspBI CTAG 3 cut(s) 77, 587, 728
GluI GCNGC 5 cut(s) 6, 33, 54, 487, 612
GsaI CCCAGC 1 cut(s) 226
HaeIII GGCC 7 cut(s) 95, 326, 365, 427, 468, 651, 857
Hin1II CATG 5 cut(s) 50, 260, 331, 383, 690
HinfI GANTC 3 cut(s) 163, 253, 962
Hpy188I TCNGA 4 cut(s) 150, 393, 577, 757
Hpy188III TCNNGA 2 cut(s) 343, 700
HpyAV CCTTC 4 cut(s) 79, 118, 439, 685
HpyCH4III ACNGT 1 cut(s) 943
HpyCH4V TGCA 9 cut(s) 53, 421, 489, 564, 581, 665, 713, 749, 818
HpyF10VI GCNNNNNNNGC 2 cut(s) 570, 863
HpyF3I CTNAG 2 cut(s) 574, 840
Hsp92II CATG 5 cut(s) 50, 260, 331, 383, 690
Ksp22I TGATCA 2 cut(s) 153, 925
Kzo9I GATC 3 cut(s) 153, 696, 925
Lsp1109I GCAGC 5 cut(s) 17, 44, 65, 473, 623
LweI GCATC 1 cut(s) 568
MaeI CTAG 3 cut(s) 77, 587, 728
MaeIII GTNAC 4 cut(s) 67, 240, 250, 268
MalI GATC 3 cut(s) 155, 698, 927
MboI GATC 3 cut(s) 153, 696, 925
MboII GAAGA 5 cut(s) 23, 380, 521, 566, 860
MfeI CAATTG 1 cut(s) 859
MflI RGATCY 1 cut(s) 696
MhlI GDGCHC 1 cut(s) 429
MlsI TGGCCA 3 cut(s) 95, 651, 857
MluCI AATT 5 cut(s) 178, 490, 810, 859, 1001
MluNI TGGCCA 3 cut(s) 95, 651, 857
MlyI GAGTC 1 cut(s) 247
MnlI CCTC 3 cut(s) 22, 519, 751
Mox20I TGGCCA 3 cut(s) 95, 651, 857
Mph1103I ATGCAT 1 cut(s) 751
MscI TGGCCA 3 cut(s) 95, 651, 857
MseI TTAA 3 cut(s) 897, 1004, 1012
Msp20I TGGCCA 3 cut(s) 95, 651, 857
MspA1I CMGCKG 2 cut(s) 8, 35
MunI CAATTG 1 cut(s) 859
Mva1269I GAATGC 2 cut(s) 352, 866
MwoI GCNNNNNNNGC 2 cut(s) 570, 863
NdeII GATC 3 cut(s) 153, 696, 925
NlaIII CATG 5 cut(s) 50, 260, 331, 383, 690
NlaIV GGNNCC 3 cut(s) 426, 427, 910
NmuCI GTSAC 2 cut(s) 250, 268
NsiI ATGCAT 1 cut(s) 751
NspI RCATGY 2 cut(s) 50, 383
NspV TTCGAA 1 cut(s) 773
PciI ACATGT 1 cut(s) 46
PctI GAATGC 2 cut(s) 352, 866
PfeI GAWTC 2 cut(s) 163, 962
PkrI GCNGC 5 cut(s) 7, 34, 55, 488, 613
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
PscI ACATGT 1 cut(s) 46
PshBI ATTAAT 1 cut(s) 1004
PspFI CCCAGC 1 cut(s) 222
PspN4I GGNNCC 3 cut(s) 426, 427, 910
PspOMI GGGCCC 1 cut(s) 425
PspPI GGNCC 4 cut(s) 425, 426, 467, 674
PsuI RGATCY 1 cut(s) 696
PvuII CAGCTG 2 cut(s) 8, 35
RsaI GTAC 3 cut(s) 452, 636, 738
RsaNI GTAC 3 cut(s) 451, 635, 737
SaqAI TTAA 3 cut(s) 897, 1004, 1012
SatI GCNGC 5 cut(s) 6, 33, 54, 487, 612
Sau3AI GATC 3 cut(s) 153, 696, 925
Sau96I GGNCC 4 cut(s) 425, 426, 467, 674
SchI GAGTC 1 cut(s) 247
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 1 cut(s) 568
SfuI TTCGAA 1 cut(s) 773
SinI GGWCC 1 cut(s) 674
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SpeI ACTAGT 1 cut(s) 586
Sse9I AATT 5 cut(s) 178, 490, 810, 859, 1001
SspMI CTAG 3 cut(s) 77, 587, 728
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 943
TaqI TCGA 4 cut(s) 142, 161, 432, 773
TasI AATT 5 cut(s) 178, 490, 810, 859, 1001
TatI WGTACW 1 cut(s) 634
TfiI GAWTC 2 cut(s) 163, 962
Tru1I TTAA 3 cut(s) 897, 1004, 1012
Tru9I TTAA 3 cut(s) 897, 1004, 1012
TscAI CASTG 2 cut(s) 649, 963
TseFI GTSAC 2 cut(s) 250, 268
TseI GCWGC 5 cut(s) 5, 32, 53, 486, 611
Tsp45I GTSAC 2 cut(s) 250, 268
TspDTI ATGAA 5 cut(s) 178, 416, 450, 848, 1011
TspGWI ACGGA 1 cut(s) 882
TspRI CASTG 2 cut(s) 649, 963
VpaK11BI GGWCC 1 cut(s) 674
VspI ATTAAT 1 cut(s) 1004
XapI RAATTY 1 cut(s) 178
XceI RCATGY 2 cut(s) 50, 383
XcmI CCANNNNNNNNNTGG 1 cut(s) 684
XspI CTAG 3 cut(s) 77, 587, 728
Zsp2I ATGCAT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.