AT5G58787

Prokaryotic RING finger family 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007024
Physical Location & Seq
Forward (+)
23742363 .. 23744646
2284 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58787.2

Sequence Viewer

Length: 729 bp
ATGGCTAAATTATCTTTCAAGGATTCTCTTAAAGCTCTTGAAGCCGATATTCAACACGCTAATACACTAGCTTTAGATTATCCTCGGGAGAAGGATGGAGCGCGCGTTCAGATGAGATTATCATACAGCCCGACTGCTCAATTTTTTCTTTTTCTTGTACAATGGACTGATTGTAAGCTTGCTGGTTTCCTCGGTTTACTCAGAGTTCTTATTTATATGACTTATGCAGATGGGAAAACAACCATGTCGGTTTACGAAAGAAAAGCCAGTATTAGAGAGTTCCAAGCTGTGATATTACCATCTTTGTCACAACTACAAAGGGGTGTTACAGACATAGATGACAGCAAACAAAAAGAGGTATGCAAGATGCGATACAGAAAGAAGGATGAGAGCGAAATGTCGGAGATTGAGATCGAGAGGGAAGAAGAATGTGGAATCTGTATGGAGATGAACAGCAAGGTGGTGCTACCAAACTGTACTCATTCTTTATGCATCAAGTGTTACCGCGATTGGCGAGGGAGATCACAGTCATGCCCGTTCTGCAGAGACAGCCTTAAAAGAGTAGATTCAGGTGATCTCTGGATGTTTTTGGACCAAAACGATACAGTGAATCTCACTGCAATCGCTAGAGAGAACCAGAAACGATTGTTTATGTACATTGAGAAGTTACCACTAGTGGTTCCAGATCAAGTGTATGCGTCTTCTCCTTACGATTTCCATGTGAGATGA

Protein Analysis

242

Amino Acids

28.14

Weight (kDa)

7.49

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 126 - 172 4.5e-10 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 128 - 167 1.5e-07 Ring finger domain
zf-C3HC4_2 PF13923 128 - 166 5.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 128 - 167 9.9e-06 zinc-RING finger domain
zf-C3HC4 PF00097 129 - 166 4.3e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 103, 105, 507
AciI CCGC 1 cut(s) 505
AfaI GTAC 3 cut(s) 159, 478, 656
AgsI TTSAA 3 cut(s) 19, 41, 53
AhlI ACTAGT 1 cut(s) 673
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 4 cut(s) 35, 71, 178, 287
AluI AGCT 4 cut(s) 35, 71, 178, 287
Alw26I GTCTC 1 cut(s) 540
Ama87I CYCGRG 1 cut(s) 84
AspLEI GCGC 2 cut(s) 103, 105
AspS9I GGNCC 1 cut(s) 592
AsuHPI GGTGA 1 cut(s) 584
AvaI CYCGRG 1 cut(s) 84
AvaII GGWCC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 693
BccI CCATC 3 cut(s) 89, 224, 307
BcoDI GTCTC 1 cut(s) 540
BcuI ACTAGT 1 cut(s) 673
BfaI CTAG 3 cut(s) 68, 627, 674
BfmI CTRYAG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 592
BmeT110I CYCGRG 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 592
BmiI GGNNCC 1 cut(s) 681
BmsI GCATC 2 cut(s) 357, 501
BpiI GAAGAC 1 cut(s) 693
BsaBI GATNNNNATC 1 cut(s) 410
BsaJI CCNNGG 2 cut(s) 83, 190
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bse1I ACTGG 1 cut(s) 267
Bse8I GATNNNNATC 1 cut(s) 410
BseDI CCNNGG 2 cut(s) 83, 190
BseGI GGATG 3 cut(s) 100, 391, 588
BseJI GATNNNNATC 1 cut(s) 410
BseMII CTCAG 1 cut(s) 214
BseNI ACTGG 1 cut(s) 267
BsePI GCGCGC 1 cut(s) 101
Bsh1236I CGCG 3 cut(s) 103, 105, 507
BsiHKCI CYCGRG 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 540
BsoBI CYCGRG 1 cut(s) 84
Bsp1407I TGTACA 2 cut(s) 157, 654
Bsp143I GATC 4 cut(s) 411, 521, 574, 685
BspACI CCGC 1 cut(s) 505
BspCNI CTCAG 1 cut(s) 213
BspFNI CGCG 3 cut(s) 103, 105, 507
BspLI GGNNCC 1 cut(s) 681
BspMAI CTGCAG 1 cut(s) 545
BsrGI TGTACA 2 cut(s) 157, 654
BsrI ACTGG 1 cut(s) 267
BssECI CCNNGG 2 cut(s) 83, 190
BssHII GCGCGC 1 cut(s) 101
BssMI GATC 4 cut(s) 411, 521, 574, 685
Bst4CI ACNGT 3 cut(s) 476, 528, 607
BstAUI TGTACA 2 cut(s) 157, 654
BstC8I GCNNGC 2 cut(s) 103, 180
BstDEI CTNAG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 100, 391, 588
BstFNI CGCG 3 cut(s) 103, 105, 507
BstHHI GCGC 2 cut(s) 103, 105
BstKTI GATC 4 cut(s) 414, 524, 577, 688
BstMAI GTCTC 1 cut(s) 540
BstMBI GATC 4 cut(s) 411, 521, 574, 685
BstMWI GCNNNNNNNGC 3 cut(s) 41, 540, 549
BstSFI CTRYAG 1 cut(s) 541
BstUI CGCG 3 cut(s) 103, 105, 507
BstV2I GAAGAC 1 cut(s) 693
BtsCI GGATG 3 cut(s) 100, 391, 588
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 2 cut(s) 612, 615
Cac8I GCNNGC 2 cut(s) 103, 180
CfoI GCGC 2 cut(s) 103, 105
Cfr13I GGNCC 1 cut(s) 592
CseI GACGC 1 cut(s) 687
Csp6I GTAC 3 cut(s) 158, 477, 655
CviAII CATG 3 cut(s) 244, 531, 719
CviJI RGCY 9 cut(s) 5, 35, 44, 71, 129, 178, 266, 287, 552
CviKI_1 RGCY 9 cut(s) 5, 35, 44, 71, 129, 178, 266, 287, 552
CviQI GTAC 3 cut(s) 158, 477, 655
DdeI CTNAG 1 cut(s) 200
DpnI GATC 4 cut(s) 413, 523, 576, 687
DpnII GATC 4 cut(s) 411, 521, 574, 685
Eco47I GGWCC 1 cut(s) 592
Eco88I CYCGRG 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 494
FaeI CATG 3 cut(s) 247, 534, 722
FatI CATG 3 cut(s) 243, 530, 718
FokI GGATG 3 cut(s) 107, 398, 595
FspBI CTAG 3 cut(s) 68, 627, 674
GlaI GCGC 2 cut(s) 102, 104
HgaI GACGC 1 cut(s) 687
HhaI GCGC 2 cut(s) 103, 105
Hin1II CATG 3 cut(s) 247, 534, 722
Hin6I GCGC 2 cut(s) 101, 103
HinP1I GCGC 2 cut(s) 101, 103
HindIII AAGCTT 1 cut(s) 176
HinfI GANTC 4 cut(s) 23, 435, 566, 610
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 2 cut(s) 197, 253
Hpy188I TCNGA 3 cut(s) 111, 203, 403
Hpy188III TCNNGA 5 cut(s) 38, 86, 415, 580, 683
Hpy8I GTNNAC 2 cut(s) 197, 253
HpyAV CCTTC 2 cut(s) 85, 376
HpyCH4III ACNGT 3 cut(s) 476, 528, 607
HpyCH4V TGCA 5 cut(s) 227, 363, 492, 543, 620
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 540, 549
HpyF3I CTNAG 1 cut(s) 200
Hsp92II CATG 3 cut(s) 247, 534, 722
HspAI GCGC 2 cut(s) 101, 103
Kzo9I GATC 4 cut(s) 411, 521, 574, 685
LmnI GCTCC 1 cut(s) 98
LpnPI CCDG 6 cut(s) 168, 280, 555, 565, 650, 696
LweI GCATC 2 cut(s) 357, 501
MaeI CTAG 3 cut(s) 68, 627, 674
MaeIII GTNAC 4 cut(s) 306, 325, 500, 666
MalI GATC 4 cut(s) 413, 523, 576, 687
MboI GATC 4 cut(s) 411, 521, 574, 685
MboII GAAGA 3 cut(s) 434, 437, 693
MluCI AATT 2 cut(s) 8, 140
MmeI TCCRAC 1 cut(s) 381
MnlI CCTC 5 cut(s) 93, 200, 349, 411, 509
Mph1103I ATGCAT 1 cut(s) 494
MseI TTAA 2 cut(s) 30, 555
MslI CAYNNNNRTG 1 cut(s) 529
MvnI CGCG 3 cut(s) 103, 105, 507
MwoI GCNNNNNNNGC 3 cut(s) 41, 540, 549
NdeII GATC 4 cut(s) 411, 521, 574, 685
NlaIII CATG 3 cut(s) 247, 534, 722
NlaIV GGNNCC 1 cut(s) 681
NmuCI GTSAC 1 cut(s) 306
NsiI ATGCAT 1 cut(s) 494
PauI GCGCGC 1 cut(s) 101
PfeI GAWTC 4 cut(s) 23, 435, 566, 610
PspN4I GGNNCC 1 cut(s) 681
PspPI GGNCC 1 cut(s) 592
PstI CTGCAG 1 cut(s) 545
PteI GCGCGC 1 cut(s) 101
RsaI GTAC 3 cut(s) 159, 478, 656
RsaNI GTAC 3 cut(s) 158, 477, 655
RseI CAYNNNNRTG 1 cut(s) 529
SaqAI TTAA 2 cut(s) 30, 555
Sau3AI GATC 4 cut(s) 411, 521, 574, 685
Sau96I GGNCC 1 cut(s) 592
SetI ASST 7 cut(s) 37, 73, 180, 289, 360, 462, 574
SfaNI GCATC 2 cut(s) 357, 501
SfcI CTRYAG 1 cut(s) 541
SinI GGWCC 1 cut(s) 592
SmiMI CAYNNNNRTG 1 cut(s) 529
SpeI ACTAGT 1 cut(s) 673
Sse9I AATT 2 cut(s) 8, 140
SsiI CCGC 1 cut(s) 505
SspMI CTAG 3 cut(s) 68, 627, 674
TaaI ACNGT 3 cut(s) 476, 528, 607
TaqI TCGA 1 cut(s) 414
TasI AATT 2 cut(s) 8, 140
TatI WGTACW 3 cut(s) 157, 476, 654
TfiI GAWTC 4 cut(s) 23, 435, 566, 610
Tru1I TTAA 2 cut(s) 30, 555
Tru9I TTAA 2 cut(s) 30, 555
TscAI CASTG 2 cut(s) 612, 622
TseFI GTSAC 1 cut(s) 306
Tsp45I GTSAC 1 cut(s) 306
TspDTI ATGAA 1 cut(s) 464
TspRI CASTG 2 cut(s) 612, 622
VpaK11BI GGWCC 1 cut(s) 592
XspI CTAG 3 cut(s) 68, 627, 674
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.