AT5G60580

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007726
Physical Location & Seq
Forward (+)
24353562 .. 24356974
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60580.1

Sequence Viewer

Length: 1464 bp
ATGGCTACTGAAGAGAAGCCTCTTAATTCTCTTGATTTAAGCCATGATGATTCTTCCCCTGCTTCAAATCAGGCTGAAGGATCATCTGCTATAACTGAAGACACTTCTGCGAATGTTCAACAATGGAGGCGAAAGAATCTCTCTTTACAGATACCCTCTAGAGCAGCTGGTCTCTCTCCTGAAGATTCGGTTGTTATCAAAATGCCTCCAACGCCTAGTCCAACTCCAAGAAGAGTCAACTTTGCTTTGACTTCGAGTTCTCCAGGTCCTACTCCTACTTCTTCTTCTGTTCTTCCTCGTGGCAAATCGTCCCTGAAGAATCTATTGCCAAAAGCTGGGTGTAAACCAAAAACCTCTAACACTGATATCGAGAAGGGACAAGGGAATGCCTGTTCTCCTCCTGCATCGCAGGAGAAGGCTTCGATATCGAGGTCATTATCACTTTCAAAGCTATTTACACCTAGGATCAAGAGAACTTCGTCCCTACCTGTGACTCCTGTTATTCTTTCAAACTCAGAGTCAGCTCACGGAGGAACCAGTGTTGCTCCTCAAACTCCAAACAGAAAAGGGAGTGTACACATAGCTCGCTCTCGCTCTGTGCCCCTTAACGACAAGGAATTAAGCCTGAAGGGAATGGATTCATTTTTCAGAGTAATTCCTTCGACTCCTCGTGTTAAGGAAGGAGACGTTTTCTCAAATGCATCAGAGGCTGGTAATACTGAAACAGGTGATGCTGATGGAGAAGACATACCTGAGGATGAAGCAGTTTGTAGGATTTGTTTGGTAGAGCTCTGTGAAGGAGGAGAAACCTTAAAAATGGAGTGTAGTTGCAAAGGCGAACTTGCTCTTGCCCACAAAGATTGTGCTCTTAAATGGTTCACCATAAAGGGTAACAAGACTTGTGAGGTGTGTAAACAAGAAGTTAAGAACTTACCTGTAACACTCTTACGCATCCAAAGCCTTCGAAATTCTGGTGTTCCTCAGCTAGATGTCTCTGGCTATAGGGTGTGGCAGGAGGTACCGGTTCTAGTAATCATCAGCATGCTCGCTTACTTCTGCTTCCTCGAGCAGCTCCTGGTTGAGAATATGGGTACAGGTGCCATCGCTATATCACTGCCGTTTTCTTGTATTCTTGGTCTTCTTGCATCCATGACCGCATCAACCATGGTAATGAGAAGATTTGTCTGGATTTACGCATCTGTCCAGTTTGCGTTGGTCGTTCTCTTCGCCCATATATTTTACTCTGTGGTGAAGTTGCAACCAGTTCTGTCAGTTCTTCTGTCAACATTTGCTGGATTTGGTGTATGCATATGCGGAAGTTCAGTGATGGTTGAGTTTGTGAGATGGAGACGAAGATGGCGAGCCAGAAGGCTAGAGCAACAGCTGAACCATGCTTTGACTCTGTCACAACCGCCGCAACCACTGGATCCAACAACCTCTCTGCATCATTCAAATACCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

487

Amino Acids

52.85

Weight (kDa)

8.87

Isoelectric Point (pI)

60.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 257 - 304 1.8e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1018
AccB1I GGYRCC 2 cut(s) 1018, 1097
AccB7I CCANNNNNTGG 1 cut(s) 335
AciI CCGC 4 cut(s) 1155, 1314, 1412, 1415
AclWI GGATC 4 cut(s) 88, 473, 1421, 1434
AcsI RAATTY 1 cut(s) 967
AcuI CTGAAG 6 cut(s) 30, 96, 117, 201, 335, 647
AfaI GTAC 3 cut(s) 576, 1020, 1093
AfiI CCNNNNNNNGG 1 cut(s) 335
AgeI ACCGGT 1 cut(s) 1021
AgsI TTSAA 5 cut(s) 66, 119, 447, 510, 1452
AjnI CCWGG 2 cut(s) 262, 1074
AloI GAACNNNNNNTCC 2 cut(s) 376, 408
AluBI AGCT 9 cut(s) 167, 335, 451, 524, 584, 790, 985, 1072, 1384
AluI AGCT 9 cut(s) 167, 335, 451, 524, 584, 790, 985, 1072, 1384
Alw21I GWGCWC 2 cut(s) 792, 868
Alw26I GTCTC 4 cut(s) 176, 678, 997, 1342
AlwI GGATC 4 cut(s) 88, 473, 1421, 1434
AlwNI CAGNNNCTG 2 cut(s) 710, 1075
Ama87I CYCGRG 1 cut(s) 1064
ApeKI GCWGC 2 cut(s) 164, 1069
ApoI RAATTY 1 cut(s) 967
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
AsiGI ACCGGT 1 cut(s) 1021
Asp700I GAANNNNTTC 1 cut(s) 637
Asp718I GGTACC 1 cut(s) 1018
AspA2I CCTAGG 1 cut(s) 461
AspS9I GGNCC 1 cut(s) 266
AsuHPI GGTGA 3 cut(s) 740, 871, 1261
AsuII TTCGAA 1 cut(s) 964
AvaI CYCGRG 1 cut(s) 1064
AvaII GGWCC 1 cut(s) 266
AvrII CCTAGG 1 cut(s) 461
AxyI CCTNAGG 1 cut(s) 753
BaeGI GKGCMC 1 cut(s) 603
BamHI GGATCC 1 cut(s) 1426
BanI GGYRCC 2 cut(s) 1018, 1097
BanII GRGCYC 1 cut(s) 792
BauI CACGAG 2 cut(s) 297, 669
BbsI GAAGAC 3 cut(s) 105, 750, 1130
Bbv12I GWGCWC 2 cut(s) 792, 868
BbvCI CCTCAGC 1 cut(s) 981
BbvI GCAGC 2 cut(s) 176, 1081
BccI CCATC 5 cut(s) 731, 1109, 1321, 1338, 1350
BceAI ACGGC 1 cut(s) 1102
BciT130I CCWGG 2 cut(s) 264, 1076
BcoDI GTCTC 4 cut(s) 176, 678, 997, 1342
BfaI CTAG 6 cut(s) 159, 216, 462, 986, 1028, 1373
BfmI CTRYAG 1 cut(s) 1000
BisI GCNGC 3 cut(s) 165, 1070, 1415
BlnI CCTAGG 1 cut(s) 461
BlsI GCNGC 3 cut(s) 166, 1071, 1416
Bme1390I CCNGG 2 cut(s) 264, 1076
Bme18I GGWCC 1 cut(s) 266
BmeT110I CYCGRG 1 cut(s) 1064
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 4 cut(s) 535, 1020, 1099, 1428
BmrFI CCNGG 2 cut(s) 264, 1076
BmsI GCATC 8 cut(s) 413, 710, 721, 960, 1154, 1166, 1205, 1453
BpiI GAAGAC 3 cut(s) 105, 750, 1130
BpmI CTGGAG 1 cut(s) 246
Bpu10I CCTNAGC 1 cut(s) 981
Bpu14I TTCGAA 1 cut(s) 964
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 2 cut(s) 461, 1164
BsaWI WCCGGW 1 cut(s) 1021
Bsc4I CCNNNNNNNGG 1 cut(s) 335
Bse118I RCCGGY 1 cut(s) 1021
Bse1I ACTGG 4 cut(s) 537, 1204, 1262, 1428
Bse21I CCTNAGG 1 cut(s) 753
BseBI CCWGG 2 cut(s) 264, 1076
BseDI CCNNGG 2 cut(s) 461, 1164
BseGI GGATG 3 cut(s) 763, 951, 1145
BseLI CCNNNNNNNGG 1 cut(s) 335
BseMII CTCAG 3 cut(s) 528, 744, 995
BseNI ACTGG 4 cut(s) 537, 1204, 1262, 1428
BseRI GAGGAG 4 cut(s) 387, 537, 657, 816
BseSI GKGCMC 1 cut(s) 603
BseXI GCAGC 2 cut(s) 176, 1081
BseYI CCCAGC 1 cut(s) 335
BshNI GGYRCC 2 cut(s) 1018, 1097
BshTI ACCGGT 1 cut(s) 1021
BsiHKAI GWGCWC 2 cut(s) 792, 868
BsiHKCI CYCGRG 1 cut(s) 1064
BsiSI CCGG 1 cut(s) 1022
BslFI GGGAC 3 cut(s) 295, 390, 466
BslI CCNNNNNNNGG 1 cut(s) 335
BsmAI GTCTC 4 cut(s) 176, 678, 997, 1342
BsmBI CGTCTC 2 cut(s) 678, 1342
BsmFI GGGAC 3 cut(s) 295, 390, 466
BsmI GAATGC 1 cut(s) 391
Bso31I GGTCTC 1 cut(s) 176
BsoBI CYCGRG 1 cut(s) 1064
Bsp119I TTCGAA 1 cut(s) 964
Bsp1286I GDGCHC 3 cut(s) 603, 792, 868
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 3 cut(s) 80, 465, 1426
Bsp19I CCATGG 1 cut(s) 1164
BspACI CCGC 4 cut(s) 1155, 1314, 1412, 1415
BspCNI CTCAG 3 cut(s) 527, 745, 994
BspLI GGNNCC 4 cut(s) 535, 1020, 1099, 1428
BspPI GGATC 4 cut(s) 88, 473, 1421, 1434
BspT104I TTCGAA 1 cut(s) 964
BspT107I GGYRCC 2 cut(s) 1018, 1097
BspTNI GGTCTC 1 cut(s) 176
BsrFI RCCGGY 1 cut(s) 1021
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 4 cut(s) 537, 1204, 1262, 1428
BssAI RCCGGY 1 cut(s) 1021
BssECI CCNNGG 2 cut(s) 461, 1164
BssMI GATC 3 cut(s) 80, 465, 1426
BssSI CACGAG 2 cut(s) 297, 669
BssT1I CCWWGG 2 cut(s) 461, 1164
Bst2BI CACGAG 2 cut(s) 297, 669
Bst2UI CCWGG 2 cut(s) 264, 1076
Bst6I CTCTTC 3 cut(s) 6, 226, 1229
BstAUI TGTACA 1 cut(s) 574
BstBI TTCGAA 1 cut(s) 964
BstC8I GCNNGC 4 cut(s) 586, 1043, 1047, 1362
BstDEI CTNAG 3 cut(s) 514, 753, 981
BstDSI CCRYGG 1 cut(s) 1164
BstF5I GGATG 3 cut(s) 763, 951, 1145
BstKTI GATC 3 cut(s) 83, 468, 1429
BstMAI GTCTC 4 cut(s) 176, 678, 997, 1342
BstMBI GATC 3 cut(s) 80, 465, 1426
BstMWI GCNNNNNNNGC 3 cut(s) 211, 707, 957
BstNI CCWGG 2 cut(s) 264, 1076
BstNSI RCATGY 1 cut(s) 1045
BstSCI CCNGG 2 cut(s) 262, 1074
BstSFI CTRYAG 1 cut(s) 1000
BstSLI GKGCMC 1 cut(s) 603
BstV1I GCAGC 2 cut(s) 176, 1081
BstV2I GAAGAC 3 cut(s) 105, 750, 1130
BstX2I RGATCY 1 cut(s) 1426
BstYI RGATCY 1 cut(s) 1426
Bsu36I CCTNAGG 1 cut(s) 753
BtgI CCRYGG 1 cut(s) 1164
BtgZI GCGATG 2 cut(s) 390, 1087
BtsCI GGATG 3 cut(s) 763, 951, 1145
BtsI GCAGTG 1 cut(s) 1112
BtsIMutI CAGTG 5 cut(s) 360, 544, 1112, 1329, 1421
Cac8I GCNNGC 4 cut(s) 586, 1043, 1047, 1362
CaiI CAGNNNCTG 2 cut(s) 710, 1075
Cfr10I RCCGGY 1 cut(s) 1021
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 3 cut(s) 575, 1019, 1092
CspAI ACCGGT 1 cut(s) 1021
CspCI CAANNNNNGTGG 2 cut(s) 842, 877
CviAII CATG 5 cut(s) 44, 1042, 1150, 1165, 1391
CviQI GTAC 3 cut(s) 575, 1019, 1092
DdeI CTNAG 3 cut(s) 514, 753, 981
DpnI GATC 3 cut(s) 82, 467, 1428
DpnII GATC 3 cut(s) 80, 465, 1426
Eam1104I CTCTTC 3 cut(s) 6, 226, 1229
EarI CTCTTC 3 cut(s) 6, 226, 1229
Ecl136II GAGCTC 1 cut(s) 790
Eco130I CCWWGG 2 cut(s) 461, 1164
Eco24I GRGCYC 1 cut(s) 792
Eco31I GGTCTC 1 cut(s) 176
Eco32I GATATC 2 cut(s) 367, 426
Eco47I GGWCC 1 cut(s) 266
Eco53kI GAGCTC 1 cut(s) 790
Eco57I CTGAAG 6 cut(s) 30, 96, 117, 201, 335, 647
Eco81I CCTNAGG 1 cut(s) 753
Eco88I CYCGRG 1 cut(s) 1064
EcoICRI GAGCTC 1 cut(s) 790
EcoO109I RGGNCCY 1 cut(s) 266
EcoRII CCWGG 2 cut(s) 262, 1074
EcoRV GATATC 2 cut(s) 367, 426
EcoT14I CCWWGG 2 cut(s) 461, 1164
EcoT22I ATGCAT 2 cut(s) 703, 1310
EcoT38I GRGCYC 1 cut(s) 792
ErhI CCWWGG 2 cut(s) 461, 1164
Esp3I CGTCTC 2 cut(s) 678, 1342
FaeI CATG 5 cut(s) 47, 1045, 1153, 1168, 1394
FalI AAGNNNNNCTT 2 cut(s) 825, 857
FaqI GGGAC 3 cut(s) 295, 390, 466
FatI CATG 5 cut(s) 43, 1041, 1149, 1164, 1390
FauNDI CATATG 1 cut(s) 1310
Fnu4HI GCNGC 3 cut(s) 165, 1070, 1415
FokI GGATG 3 cut(s) 770, 938, 1132
FriOI GRGCYC 1 cut(s) 792
Fsp4HI GCNGC 3 cut(s) 165, 1070, 1415
FspBI CTAG 6 cut(s) 159, 216, 462, 986, 1028, 1373
GluI GCNGC 3 cut(s) 165, 1070, 1415
GsaI CCCAGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 246
HapII CCGG 1 cut(s) 1022
Hin1II CATG 5 cut(s) 47, 1045, 1153, 1168, 1394
HincII GTYRAC 2 cut(s) 238, 1284
HindII GTYRAC 2 cut(s) 238, 1284
HpaII CCGG 1 cut(s) 1022
HphI GGTGA 3 cut(s) 740, 871, 1261
Hpy166II GTNNAC 7 cut(s) 238, 344, 575, 577, 879, 914, 1284
Hpy188I TCNGA 3 cut(s) 517, 650, 706
Hpy188III TCNNGA 6 cut(s) 32, 159, 179, 370, 469, 1186
Hpy8I GTNNAC 7 cut(s) 238, 344, 575, 577, 879, 914, 1284
HpyAV CCTTC 9 cut(s) 71, 367, 409, 622, 669, 674, 791, 971, 1362
HpyCH4IV ACGT 1 cut(s) 687
HpyCH4V TGCA 7 cut(s) 404, 701, 831, 1145, 1258, 1308, 1444
HpyF10VI GCNNNNNNNGC 3 cut(s) 211, 707, 957
HpyF3I CTNAG 3 cut(s) 514, 753, 981
HpySE526I ACGT 1 cut(s) 687
Hsp92II CATG 5 cut(s) 47, 1045, 1153, 1168, 1394
KpnI GGTACC 1 cut(s) 1022
Kzo9I GATC 3 cut(s) 80, 465, 1426
LmnI GCTCC 2 cut(s) 550, 1077
Lsp1109I GCAGC 2 cut(s) 176, 1081
LweI GCATC 8 cut(s) 413, 710, 721, 960, 1154, 1166, 1205, 1453
MaeI CTAG 6 cut(s) 159, 216, 462, 986, 1028, 1373
MaeII ACGT 1 cut(s) 687
MaeIII GTNAC 4 cut(s) 490, 890, 937, 1404
MalI GATC 3 cut(s) 82, 467, 1428
MboI GATC 3 cut(s) 80, 465, 1426
MflI RGATCY 1 cut(s) 1426
MhlI GDGCHC 3 cut(s) 603, 792, 868
MluCI AATT 4 cut(s) 25, 617, 654, 967
MlyI GAGTC 5 cut(s) 243, 487, 527, 658, 1393
MmeI TCCRAC 3 cut(s) 233, 245, 1454
Mph1103I ATGCAT 2 cut(s) 703, 1310
MroXI GAANNNNTTC 1 cut(s) 637
MseI TTAA 8 cut(s) 24, 38, 606, 620, 675, 812, 870, 924
MslI CAYNNNNRTG 2 cut(s) 1040, 1169
MspA1I CMGCKG 2 cut(s) 167, 1384
MspI CCGG 1 cut(s) 1022
MspR9I CCNGG 2 cut(s) 264, 1076
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 2 cut(s) 264, 1076
MwoI GCNNNNNNNGC 3 cut(s) 211, 707, 957
NcoI CCATGG 1 cut(s) 1164
NdeI CATATG 1 cut(s) 1310
NdeII GATC 3 cut(s) 80, 465, 1426
NlaIII CATG 5 cut(s) 47, 1045, 1153, 1168, 1394
NlaIV GGNNCC 4 cut(s) 535, 1020, 1099, 1428
NmuCI GTSAC 2 cut(s) 490, 1404
NsiI ATGCAT 2 cut(s) 703, 1310
NspI RCATGY 1 cut(s) 1045
NspV TTCGAA 1 cut(s) 964
PaeI GCATGC 1 cut(s) 1045
PaeR7I CTCGAG 1 cut(s) 1064
PcsI WCGNNNNNNNCGW 1 cut(s) 1357
PctI GAATGC 1 cut(s) 391
PdmI GAANNNNTTC 1 cut(s) 637
PfeI GAWTC 5 cut(s) 50, 136, 185, 319, 638
PflFI GACNNNGTC 1 cut(s) 1402
PflMI CCANNNNNTGG 1 cut(s) 335
PinAI ACCGGT 1 cut(s) 1021
PkrI GCNGC 3 cut(s) 166, 1071, 1416
PleI GAGTC 5 cut(s) 242, 487, 526, 658, 1393
PpsI GAGTC 5 cut(s) 242, 487, 526, 658, 1393
PpuMI RGGWCCY 1 cut(s) 266
Psp124BI GAGCTC 1 cut(s) 792
Psp5II RGGWCCY 1 cut(s) 266
Psp6I CCWGG 2 cut(s) 262, 1074
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 2 cut(s) 262, 1074
PspN4I GGNNCC 4 cut(s) 535, 1020, 1099, 1428
PspPI GGNCC 1 cut(s) 266
PspPPI RGGWCCY 1 cut(s) 266
PspXI VCTCGAGB 1 cut(s) 1064
PstNI CAGNNNCTG 2 cut(s) 710, 1075
PsuI RGATCY 1 cut(s) 1426
PsyI GACNNNGTC 1 cut(s) 1402
PvuII CAGCTG 2 cut(s) 167, 1384
RsaI GTAC 3 cut(s) 576, 1020, 1093
RsaNI GTAC 3 cut(s) 575, 1019, 1092
RseI CAYNNNNRTG 2 cut(s) 1040, 1169
SacI GAGCTC 1 cut(s) 792
SaqAI TTAA 8 cut(s) 24, 38, 606, 620, 675, 812, 870, 924
SatI GCNGC 3 cut(s) 165, 1070, 1415
Sau3AI GATC 3 cut(s) 80, 465, 1426
Sau96I GGNCC 1 cut(s) 266
SchI GAGTC 5 cut(s) 243, 487, 527, 658, 1393
ScrFI CCNGG 2 cut(s) 264, 1076
SduI GDGCHC 3 cut(s) 603, 792, 868
SfaNI GCATC 8 cut(s) 413, 710, 721, 960, 1154, 1166, 1205, 1453
SfcI CTRYAG 1 cut(s) 1000
Sfr274I CTCGAG 1 cut(s) 1064
SfuI TTCGAA 1 cut(s) 964
SinI GGWCC 1 cut(s) 266
SlaI CTCGAG 1 cut(s) 1064
SmiMI CAYNNNNRTG 2 cut(s) 1040, 1169
SmlI CTYRAG 1 cut(s) 1064
SmoI CTYRAG 1 cut(s) 1064
SphI GCATGC 1 cut(s) 1045
Sse9I AATT 4 cut(s) 25, 617, 654, 967
SsiI CCGC 4 cut(s) 1155, 1314, 1412, 1415
SspMI CTAG 6 cut(s) 159, 216, 462, 986, 1028, 1373
SstI GAGCTC 1 cut(s) 792
StyD4I CCNGG 2 cut(s) 262, 1074
StyI CCWWGG 2 cut(s) 461, 1164
TaiI ACGT 1 cut(s) 690
TaqI TCGA 7 cut(s) 254, 369, 422, 428, 662, 964, 1065
TasI AATT 4 cut(s) 25, 617, 654, 967
TatI WGTACW 1 cut(s) 574
TauI GCSGC 1 cut(s) 1417
TfiI GAWTC 5 cut(s) 50, 136, 185, 319, 638
Tru1I TTAA 8 cut(s) 24, 38, 606, 620, 675, 812, 870, 924
Tru9I TTAA 8 cut(s) 24, 38, 606, 620, 675, 812, 870, 924
TscAI CASTG 5 cut(s) 367, 544, 1119, 1329, 1428
TseFI GTSAC 2 cut(s) 490, 1404
TseI GCWGC 2 cut(s) 164, 1069
Tsp45I GTSAC 2 cut(s) 490, 1404
TspDTI ATGAA 2 cut(s) 630, 774
TspGWI ACGGA 1 cut(s) 543
TspRI CASTG 5 cut(s) 367, 544, 1119, 1329, 1428
Tth111I GACNNNGTC 1 cut(s) 1402
Van91I CCANNNNNTGG 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 266
XapI RAATTY 1 cut(s) 967
XbaI TCTAGA 1 cut(s) 158
XceI RCATGY 1 cut(s) 1045
XhoI CTCGAG 1 cut(s) 1064
XmaJI CCTAGG 1 cut(s) 461
XmnI GAANNNNTTC 1 cut(s) 637
XspI CTAG 6 cut(s) 159, 216, 462, 986, 1028, 1373
Zsp2I ATGCAT 2 cut(s) 703, 1310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.