MD03G1116800.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007726
Physical Location & Seq
Forward (+)
10490643 .. 10495647
5005 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1116800.v1.1.491

Sequence Viewer

Length: 1389 bp
ATGGGAACTGAGGAAGAAAAGCATGATCATGATCAAAAATCATGCCAAGGGCAAGGAGAAGTTGTTGTGCCACTTCACAAGGTTGAAGATTCAGTAGGAGTAGCTGAAGAAGCACCCCAAGTTCATCCATGGAAGCGACAAAATCTCCACCTGGAGATACCCACGAGAACATCCGAAGATTGTGTCAAGGATTTTGTTGCAATAAAGATGCCTCCAACACCAAGTCCTACTCCTAGGAAAGTGAATTTTCTTTTGACACCTGGTTCTGTAGACGGAAGAATGAGTGATTCGCCAGTGCCCTCTTCAGCAAAAGGCAAATCATCTATAAAATCTATTTTTCCAAAACTAAGCTTCAAGTATCGAAGTTCTGCTGATGCCGAGAAGGCGTCCAACCTAGCTGCAGAAGGTTCGTCTACAGGAGCTCGGGAGAAAGCTTCCATCTCAAGGTCACTGTCTCTCACAAAACTATTCACTCCGAGGATGAAGACAACATCATCGCTACCTGTAACTCCAATTGCACTTGCACACCGAGAATATGCACATGGCGGAAGTGTTGGTGGCTCTCTGTATTCAATTAGGAAAGAATCCCGAAGACAGATAGCTCGCTCACTTTCAGTCCCAGTCAACCATAAAGAAATGAACTTAAAGATGATGGATTCATTTTTCCGTGTAATTCCTTCAACTCCTCGAGTCAAGGAAGGGGATGGTGTATCGATTAGTTCCCCAACAGTAGATGCTGATGATGATAATGGTGAAGAGATACCGGAGAAAGAGGCCGTCTGTAGAATTTGTCTAGTGGAACTGTGTGAAGGGGGTGAGACCCTCAAAATGGAATGCAGTTGCAAAGGCGAACTTGCTCTGGCCCACCAAGAATGTGCTATTAAATGGTTTAGCATCAAAGGTAACAAGACGTGTGATGTTTGTAAGCAAGAGGTTCAAAACCTGCCTGTCACTCTATTACGAATTCAAAGTGTTCGAGCTCGAAATGCTGGAGCAAGTCTGGTTTGGCAGGAAGTGCCTGTGCTTGTCATTGTCAGCATGCTTGCCTACTTTTGTTTTCTTGAACAGCTCCTGGTGGAGAAGATGGGTACTGGAGCAATTGCCATCTCTCTTCCATTTTCTTGTGTACTGGGTCTTCTCTCATCCGTGACTTCATCAACCATGGTGAAAAGAAGATTTGTTTGGGTTTACGCATCGGTTCAGTTTGCTCTGGTCGTTCTATTTGCACATATATTTTACTCTTTGGTCCGCGTGCAAGCAGTTCTTTGTATTCTCCTCGCAACATTTGCTGGGTTGGGAGTCGCAATAAGTGGGAGTTCCATTATTGTCGAGTTCTTGAGATGGAGAAGAAGATGGCAAACTGCGTCTGGGCAACGCCAGATGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

463

Amino Acids

50.66

Weight (kDa)

8.95

Isoelectric Point (pI)

54.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 261 - 308 2.9e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 951
AccI GTMKAC 2 cut(s) 270, 413
AccII CGCG 1 cut(s) 1251
AciI CCGC 2 cut(s) 546, 1249
AcsI RAATTY 3 cut(s) 244, 786, 963
AcuI CTGAAG 2 cut(s) 126, 288
AcyI GRCGYC 1 cut(s) 386
AfaI GTAC 2 cut(s) 1090, 1128
AfiI CCNNNNNNNGG 2 cut(s) 444, 829
AflIII ACRYGT 1 cut(s) 911
AgsI TTSAA 7 cut(s) 86, 355, 573, 681, 938, 968, 1064
AjiI CACGTC 1 cut(s) 912
AjnI CCWGG 3 cut(s) 150, 259, 1071
AjuI GAANNNNNNNTTGG 2 cut(s) 1165, 1197
AluBI AGCT 8 cut(s) 104, 351, 398, 422, 434, 602, 980, 1069
AluI AGCT 8 cut(s) 104, 351, 398, 422, 434, 602, 980, 1069
Alw21I GWGCWC 2 cut(s) 424, 982
Alw26I GTCTC 2 cut(s) 459, 812
AlwNI CAGNNNCTG 1 cut(s) 1072
Ama87I CYCGRG 2 cut(s) 423, 687
AoxI GGCC 2 cut(s) 774, 861
ApeKI GCWGC 1 cut(s) 398
ApoI RAATTY 3 cut(s) 244, 786, 963
ArsI GACNNNNNNTTYG 2 cut(s) 168, 200
AspA2I CCTAGG 1 cut(s) 233
AspS9I GGNCC 2 cut(s) 862, 1246
AsuHPI GGTGA 3 cut(s) 764, 827, 1177
AvaI CYCGRG 2 cut(s) 423, 687
AvaII GGWCC 1 cut(s) 1246
AvrII CCTAGG 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 300
BanII GRGCYC 2 cut(s) 424, 982
BarI GAAGNNNNNNTAC 2 cut(s) 1119, 1151
BauI CACGAG 1 cut(s) 163
BbsI GAAGAC 3 cut(s) 491, 598, 1127
Bbv12I GWGCWC 2 cut(s) 424, 982
BbvI GCAGC 1 cut(s) 385
BccI CCATC 7 cut(s) 446, 646, 698, 1078, 1112, 1335, 1347
BceAI ACGGC 1 cut(s) 761
BcgI CGANNNNNNTGC 2 cut(s) 390, 424
BciT130I CCWGG 3 cut(s) 152, 261, 1073
BclI TGATCA 2 cut(s) 25, 31
BcoDI GTCTC 2 cut(s) 459, 812
BfaI CTAG 3 cut(s) 234, 395, 794
BfmI CTRYAG 4 cut(s) 267, 399, 414, 781
BfuAI ACCTGC 1 cut(s) 951
BglI GCCNNNNNGGC 1 cut(s) 383
BisI GCNGC 1 cut(s) 399
BlnI CCTAGG 1 cut(s) 233
BlsI GCNGC 1 cut(s) 400
Bme1390I CCNGG 3 cut(s) 152, 261, 1073
Bme18I GGWCC 1 cut(s) 1246
BmeT110I CYCGRG 2 cut(s) 423, 687
BmgBI CACGTC 1 cut(s) 912
BmgT120I GGNCC 2 cut(s) 862, 1246
BmrFI CCNGG 3 cut(s) 152, 261, 1073
BmrI ACTGGG 2 cut(s) 614, 1139
BmsI GCATC 5 cut(s) 198, 364, 724, 903, 1202
BmuI ACTGGG 2 cut(s) 614, 1139
BpiI GAAGAC 3 cut(s) 491, 598, 1127
BpmI CTGGAG 3 cut(s) 173, 1011, 1113
BpuEI CTTGAG 2 cut(s) 427, 1357
Bsa29I ATCGAT 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 812
BsaJI CCNNGG 5 cut(s) 46, 128, 233, 476, 1161
BsaWI WCCGGW 1 cut(s) 763
BsaXI ACNNNNNCTCC 4 cut(s) 48, 78, 129, 159
Bsc4I CCNNNNNNNGG 2 cut(s) 444, 829
Bse1I ACTGG 4 cut(s) 293, 620, 1096, 1134
Bse8I GATNNNNATC 1 cut(s) 30
BseBI CCWGG 3 cut(s) 152, 261, 1073
BseCI ATCGAT 1 cut(s) 713
BseDI CCNNGG 5 cut(s) 46, 128, 233, 476, 1161
BseGI GGATG 5 cut(s) 124, 170, 486, 709, 1142
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 444, 829
BseNI ACTGG 4 cut(s) 293, 620, 1096, 1134
BseRI GAGGAG 2 cut(s) 675, 1265
BseSI GKGCMC 1 cut(s) 300
BseXI GCAGC 1 cut(s) 385
BseYI CCCAGC 1 cut(s) 1289
Bsh1236I CGCG 1 cut(s) 1251
BshFI GGCC 2 cut(s) 776, 863
BshVI ATCGAT 1 cut(s) 713
BsiHKAI GWGCWC 2 cut(s) 424, 982
BsiHKCI CYCGRG 2 cut(s) 423, 687
BsiSI CCGG 1 cut(s) 764
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 2 cut(s) 444, 829
BsmAI GTCTC 2 cut(s) 459, 812
BsmFI GGGAC 1 cut(s) 602
BsmI GAATGC 1 cut(s) 839
BsnI GGCC 2 cut(s) 776, 863
Bso31I GGTCTC 1 cut(s) 812
BsoBI CYCGRG 2 cut(s) 423, 687
Bsp1286I GDGCHC 3 cut(s) 300, 424, 982
Bsp143I GATC 2 cut(s) 25, 31
Bsp19I CCATGG 2 cut(s) 128, 1161
BspACI CCGC 2 cut(s) 546, 1249
BspANI GGCC 2 cut(s) 776, 863
BspDI ATCGAT 1 cut(s) 713
BspFNI CGCG 1 cut(s) 1251
BspHI TCATGA 1 cut(s) 28
BspMAI CTGCAG 1 cut(s) 403
BspMI ACCTGC 1 cut(s) 951
BspTNI GGTCTC 1 cut(s) 812
BsrI ACTGG 4 cut(s) 293, 620, 1096, 1134
BssECI CCNNGG 5 cut(s) 46, 128, 233, 476, 1161
BssMI GATC 2 cut(s) 25, 31
BssNI GRCGYC 1 cut(s) 386
BssSI CACGAG 1 cut(s) 163
BssT1I CCWWGG 4 cut(s) 46, 128, 233, 1161
Bst2BI CACGAG 1 cut(s) 163
Bst2UI CCWGG 3 cut(s) 152, 261, 1073
Bst4CI ACNGT 3 cut(s) 453, 730, 804
Bst6I CTCTTC 3 cut(s) 307, 750, 1116
BstACI GRCGYC 1 cut(s) 386
BstAPI GCANNNNNTGC 2 cut(s) 1015, 1286
BstC8I GCNNGC 5 cut(s) 604, 1040, 1044, 1253, 1257
BstDEI CTNAG 2 cut(s) 9, 347
BstDSI CCRYGG 2 cut(s) 128, 1161
BstF5I GGATG 5 cut(s) 124, 170, 486, 709, 1142
BstFNI CGCG 1 cut(s) 1251
BstKTI GATC 2 cut(s) 28, 34
BstMAI GTCTC 2 cut(s) 459, 812
BstMBI GATC 2 cut(s) 25, 31
BstMWI GCNNNNNNNGC 5 cut(s) 110, 383, 986, 1015, 1286
BstNI CCWGG 3 cut(s) 152, 261, 1073
BstNSI RCATGY 1 cut(s) 1042
BstSCI CCNGG 3 cut(s) 150, 259, 1071
BstSFI CTRYAG 4 cut(s) 267, 399, 414, 781
BstSLI GKGCMC 1 cut(s) 300
BstUI CGCG 1 cut(s) 1251
BstV1I GCAGC 1 cut(s) 385
BstV2I GAAGAC 3 cut(s) 491, 598, 1127
Bsu15I ATCGAT 1 cut(s) 713
BsuRI GGCC 2 cut(s) 776, 863
BsuTUI ATCGAT 1 cut(s) 713
BtgI CCRYGG 2 cut(s) 128, 1161
BtgZI GCGATG 1 cut(s) 480
BtrI CACGTC 1 cut(s) 912
BtsCI GGATG 5 cut(s) 124, 170, 486, 709, 1142
BtsIMutI CAGTG 2 cut(s) 300, 449
BveI ACCTGC 1 cut(s) 951
Cac8I GCNNGC 5 cut(s) 604, 1040, 1044, 1253, 1257
CaiI CAGNNNCTG 1 cut(s) 1072
CciI TCATGA 1 cut(s) 28
Cfr13I GGNCC 2 cut(s) 862, 1246
ClaI ATCGAT 1 cut(s) 713
CseI GACGC 2 cut(s) 375, 1353
CsiI ACCWGGT 1 cut(s) 259
Csp6I GTAC 2 cut(s) 1089, 1127
CviAII CATG 7 cut(s) 23, 29, 42, 129, 542, 1039, 1162
CviQI GTAC 2 cut(s) 1089, 1127
DdeI CTNAG 2 cut(s) 9, 347
DpnI GATC 2 cut(s) 27, 33
DpnII GATC 2 cut(s) 25, 31
Eam1104I CTCTTC 3 cut(s) 307, 750, 1116
EarI CTCTTC 3 cut(s) 307, 750, 1116
EciI GGCGGA 1 cut(s) 561
Ecl136II GAGCTC 2 cut(s) 422, 980
Eco130I CCWWGG 4 cut(s) 46, 128, 233, 1161
Eco24I GRGCYC 2 cut(s) 424, 982
Eco31I GGTCTC 1 cut(s) 812
Eco47I GGWCC 1 cut(s) 1246
Eco53kI GAGCTC 2 cut(s) 422, 980
Eco57I CTGAAG 2 cut(s) 126, 288
Eco88I CYCGRG 2 cut(s) 423, 687
EcoICRI GAGCTC 2 cut(s) 422, 980
EcoRI GAATTC 1 cut(s) 963
EcoRII CCWGG 3 cut(s) 150, 259, 1071
EcoT14I CCWWGG 4 cut(s) 46, 128, 233, 1161
EcoT38I GRGCYC 2 cut(s) 424, 982
ErhI CCWWGG 4 cut(s) 46, 128, 233, 1161
FaeI CATG 7 cut(s) 26, 32, 45, 132, 545, 1042, 1165
FalI AAGNNNNNCTT 4 cut(s) 837, 869, 1248, 1280
FaqI GGGAC 1 cut(s) 602
FatI CATG 7 cut(s) 22, 28, 41, 128, 541, 1038, 1161
FbaI TGATCA 2 cut(s) 25, 31
FblI GTMKAC 2 cut(s) 270, 413
Fnu4HI GCNGC 1 cut(s) 399
FokI GGATG 5 cut(s) 111, 157, 493, 716, 1129
FriOI GRGCYC 2 cut(s) 424, 982
Fsp4HI GCNGC 1 cut(s) 399
FspBI CTAG 3 cut(s) 234, 395, 794
GluI GCNGC 1 cut(s) 399
GsaI CCCAGC 1 cut(s) 1293
GsuI CTGGAG 3 cut(s) 173, 1011, 1113
HaeIII GGCC 2 cut(s) 776, 863
HapII CCGG 1 cut(s) 764
HgaI GACGC 2 cut(s) 375, 1353
Hin1I GRCGYC 1 cut(s) 386
Hin1II CATG 7 cut(s) 26, 32, 45, 132, 545, 1042, 1165
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HindIII AAGCTT 2 cut(s) 349, 432
HinfI GANTC 6 cut(s) 89, 287, 584, 656, 690, 1299
HpaII CCGG 1 cut(s) 764
HphI GGTGA 3 cut(s) 764, 827, 1177
Hpy166II GTNNAC 5 cut(s) 271, 414, 625, 1127, 1189
Hpy188I TCNGA 2 cut(s) 175, 477
Hpy188III TCNNGA 5 cut(s) 29, 425, 588, 1061, 1336
Hpy8I GTNNAC 5 cut(s) 271, 414, 625, 1127, 1189
HpyAV CCTTC 5 cut(s) 376, 398, 687, 692, 803
HpyCH4III ACNGT 3 cut(s) 453, 730, 804
HpyCH4IV ACGT 1 cut(s) 911
HpyCH4V TGCA 9 cut(s) 200, 401, 518, 524, 539, 837, 843, 1226, 1255
HpyF10VI GCNNNNNNNGC 5 cut(s) 110, 383, 986, 1015, 1286
HpyF3I CTNAG 2 cut(s) 9, 347
HpySE526I ACGT 1 cut(s) 911
Hsp92I GRCGYC 1 cut(s) 386
Hsp92II CATG 7 cut(s) 26, 32, 45, 132, 545, 1042, 1165
Ksp22I TGATCA 2 cut(s) 25, 31
Kzo9I GATC 2 cut(s) 25, 31
LmnI GCTCC 4 cut(s) 419, 992, 1074, 1094
Lsp1109I GCAGC 1 cut(s) 385
LweI GCATC 5 cut(s) 198, 364, 724, 903, 1202
MabI ACCWGGT 1 cut(s) 259
MaeI CTAG 3 cut(s) 234, 395, 794
MaeII ACGT 1 cut(s) 911
MaeIII GTNAC 5 cut(s) 447, 505, 902, 949, 1147
MalI GATC 2 cut(s) 27, 33
MboI GATC 2 cut(s) 25, 31
MfeI CAATTG 2 cut(s) 513, 1098
MhlI GDGCHC 3 cut(s) 300, 424, 982
MluCI AATT 7 cut(s) 244, 513, 573, 672, 786, 963, 1098
MlyI GAGTC 2 cut(s) 699, 1308
MmeI TCCRAC 2 cut(s) 239, 414
MnlI CCTC 9 cut(s) 4, 222, 310, 471, 696, 766, 833, 925, 1286
MseI TTAA 2 cut(s) 644, 882
MslI CAYNNNNRTG 1 cut(s) 27
MspI CCGG 1 cut(s) 764
MspR9I CCNGG 3 cut(s) 152, 261, 1073
MunI CAATTG 2 cut(s) 513, 1098
Mva1269I GAATGC 1 cut(s) 839
MvaI CCWGG 3 cut(s) 152, 261, 1073
MvnI CGCG 1 cut(s) 1251
MwoI GCNNNNNNNGC 5 cut(s) 110, 383, 986, 1015, 1286
NcoI CCATGG 2 cut(s) 128, 1161
NdeII GATC 2 cut(s) 25, 31
NlaIII CATG 7 cut(s) 26, 32, 45, 132, 545, 1042, 1165
NmeAIII GCCGAG 1 cut(s) 403
NmuCI GTSAC 3 cut(s) 447, 949, 1147
NspI RCATGY 1 cut(s) 1042
PaeI GCATGC 1 cut(s) 1042
PaeR7I CTCGAG 1 cut(s) 687
PagI TCATGA 1 cut(s) 28
PctI GAATGC 1 cut(s) 839
PfeI GAWTC 4 cut(s) 89, 287, 584, 656
PkrI GCNGC 1 cut(s) 400
PleI GAGTC 2 cut(s) 698, 1307
PpsI GAGTC 2 cut(s) 698, 1307
Psp124BI GAGCTC 2 cut(s) 424, 982
Psp6I CCWGG 3 cut(s) 150, 259, 1071
PspFI CCCAGC 1 cut(s) 1289
PspGI CCWGG 3 cut(s) 150, 259, 1071
PspPI GGNCC 2 cut(s) 862, 1246
PspXI VCTCGAGB 1 cut(s) 687
PstI CTGCAG 1 cut(s) 403
PstNI CAGNNNCTG 1 cut(s) 1072
RsaI GTAC 2 cut(s) 1090, 1128
RsaNI GTAC 2 cut(s) 1089, 1127
RseI CAYNNNNRTG 1 cut(s) 27
SacI GAGCTC 2 cut(s) 424, 982
SaqAI TTAA 2 cut(s) 644, 882
SatI GCNGC 1 cut(s) 399
Sau3AI GATC 2 cut(s) 25, 31
Sau96I GGNCC 2 cut(s) 862, 1246
SchI GAGTC 2 cut(s) 699, 1308
ScrFI CCNGG 3 cut(s) 152, 261, 1073
SduI GDGCHC 3 cut(s) 300, 424, 982
SexAI ACCWGGT 1 cut(s) 259
SfaNI GCATC 5 cut(s) 198, 364, 724, 903, 1202
SfcI CTRYAG 4 cut(s) 267, 399, 414, 781
Sfr274I CTCGAG 1 cut(s) 687
SinI GGWCC 1 cut(s) 1246
SlaI CTCGAG 1 cut(s) 687
SmiMI CAYNNNNRTG 1 cut(s) 27
SmlI CTYRAG 3 cut(s) 442, 687, 1336
SmoI CTYRAG 3 cut(s) 442, 687, 1336
SphI GCATGC 1 cut(s) 1042
Sse9I AATT 7 cut(s) 244, 513, 573, 672, 786, 963, 1098
SsiI CCGC 2 cut(s) 546, 1249
SspMI CTAG 3 cut(s) 234, 395, 794
SstI GAGCTC 2 cut(s) 424, 982
StyD4I CCNGG 3 cut(s) 150, 259, 1071
StyI CCWWGG 4 cut(s) 46, 128, 233, 1161
TaaI ACNGT 3 cut(s) 453, 730, 804
TaiI ACGT 1 cut(s) 914
TaqI TCGA 6 cut(s) 361, 688, 713, 976, 982, 1329
TasI AATT 7 cut(s) 244, 513, 573, 672, 786, 963, 1098
TatI WGTACW 1 cut(s) 1126
TfiI GAWTC 4 cut(s) 89, 287, 584, 656
Tru1I TTAA 2 cut(s) 644, 882
Tru9I TTAA 2 cut(s) 644, 882
TscAI CASTG 2 cut(s) 300, 456
TseFI GTSAC 3 cut(s) 447, 949, 1147
TseI GCWGC 1 cut(s) 398
Tsp45I GTSAC 3 cut(s) 447, 949, 1147
TspDTI ATGAA 5 cut(s) 113, 497, 648, 653, 1143
TspGWI ACGGA 3 cut(s) 288, 656, 1135
TspRI CASTG 2 cut(s) 300, 456
VpaK11BI GGWCC 1 cut(s) 1246
XapI RAATTY 3 cut(s) 244, 786, 963
XceI RCATGY 1 cut(s) 1042
XhoI CTCGAG 1 cut(s) 687
XmaJI CCTAGG 1 cut(s) 233
XmiI GTMKAC 2 cut(s) 270, 413
XspI CTAG 3 cut(s) 234, 395, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.