Rh5CG424000

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0007726
Physical Location & Seq
Reverse (-)
58647795 .. 58653076
5282 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG424000.1

Sequence Viewer

Length: 1068 bp
ATGGCAACTGAGGAAAAGCATGTGGGTGATGGGAATGATGAGAATGCAAGCCAAGGGACAATTGTTGCTTCTCCAGTTGTTCAAAAGGTTGAAGACTCAGTAGGAATAACTGAAGAAACACCTCAAGTTCATCCATGGAAGCGACAAAATCTCCACCTTGAGATACCCTCAAGAACAGAAGAAGACGGAGCTCAGGATTTTGTTGCAATAAAGATGCCTCCAACACCAAGTCTCACCCCTAGGAAAGTAAATTTTAATCTTACTCCAGGTTCAAGTGATGCAAGAATTAGTGGATCTCCAGGCTCCTCTTCATCCAAAGGAAAATCTTGTAAAAGATCTCTTTTTCCGAAACTAAGCTTCAAGAATCGAAGTTCTTCAGATGTCGAGAAGGCTGCTAACCTAAATTCAGAAGGATCACCTGCAGGACCAGCGCGAGAAAAGCCTTCCATATCAAGGTCATTGTCCCTTACAAAGCTGTTCACTCCGCGGATGAAGAGAACATCATCTCTACCTGTAACTCCTCTTGCACATTCACGCCTAGAATCTACACATGGTGGAAGTGTTGGCAGCTCTCTAAATTCAGCAACAAAAGGAGCCCATCGACAGATAGCTCGCTCCCTTTCAGTTCCGATCAACCCTAAAGAAAAGGTTACCTTAAGGAGGATGGATTCTTTTTTCCGTGTAATTCCTTCCACTCCCCGAATGAAGGAAGGGGATGTAATATCGACTACTTCCCCAACAGCGGATGCTGGAAAAGATGATGACAATGGTGAAGACATATCTGAAGAAGAGGCTGTCTGCAGAATTTGTCTAGTTGAACTGTGTGAAGGGGGTGAGACCCTCAAAATGGAATGCAGTTGCAAAGGTGAACTTGCTCTGGCCCATCAGGAATGCGCTATAAAATGGTTTAGTATCAAAGGTAACAAGACCTGTGATGTATGCAAGCAAGAGGTTCTAAACCTGCCTGTCACTCTATTACGAATACAAAGTGTCCGAACTCGAAATGCTGGAAGTAGATTTCAGCAGGAAGATGCTAATGGATACAGGCAAGTGTTCAATAGGCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

355

Amino Acids

38.61

Weight (kDa)

8.8

Isoelectric Point (pI)

56.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 267 - 314 2e-11 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 427
Acc36I ACCTGC 2 cut(s) 427, 969
AccII CGCG 2 cut(s) 433, 487
AciI CCGC 3 cut(s) 485, 487, 743
AclWI GGATC 2 cut(s) 301, 421
AcsI RAATTY 4 cut(s) 250, 403, 577, 804
AcuI CTGAAG 3 cut(s) 132, 360, 804
AdeI CACNNNGTG 1 cut(s) 554
AfiI CCNNNNNNNGG 5 cut(s) 453, 660, 706, 742, 847
AflII CTTAAG 1 cut(s) 655
AgsI TTSAA 6 cut(s) 83, 92, 273, 361, 818, 1057
AjnI CCWGG 2 cut(s) 265, 298
AluBI AGCT 5 cut(s) 191, 357, 475, 570, 611
AluI AGCT 5 cut(s) 191, 357, 475, 570, 611
Alw21I GWGCWC 1 cut(s) 193
Alw26I GTCTC 2 cut(s) 236, 830
AlwI GGATC 2 cut(s) 301, 421
AoxI GGCC 1 cut(s) 879
ApeKI GCWGC 2 cut(s) 392, 567
ApoI RAATTY 4 cut(s) 250, 403, 577, 804
Asp700I GAANNNNTTC 1 cut(s) 373
AspA2I CCTAGG 1 cut(s) 239
AspLEI GCGC 2 cut(s) 433, 896
AspS9I GGNCC 2 cut(s) 425, 880
AsuHPI GGTGA 6 cut(s) 38, 226, 408, 782, 845, 878
AvaII GGWCC 1 cut(s) 425
AvrII CCTAGG 1 cut(s) 239
BanII GRGCYC 2 cut(s) 193, 598
BbsI GAAGAC 3 cut(s) 99, 189, 780
Bbv12I GWGCWC 1 cut(s) 193
BbvI GCAGC 2 cut(s) 379, 579
BccI CCATC 4 cut(s) 23, 606, 658, 891
BcgI CGANNNNNNTGC 2 cut(s) 374, 408
BciT130I CCWGG 2 cut(s) 267, 300
BciVI GTATCC 1 cut(s) 1034
BcoDI GTCTC 2 cut(s) 236, 830
BfaI CTAG 4 cut(s) 240, 539, 812, 1066
BfmI CTRYAG 2 cut(s) 420, 799
BfrI CTTAAG 1 cut(s) 655
BfuAI ACCTGC 2 cut(s) 427, 969
BfuI GTATCC 1 cut(s) 1034
BglII AGATCT 1 cut(s) 335
BisI GCNGC 2 cut(s) 393, 568
BlnI CCTAGG 1 cut(s) 239
BlsI GCNGC 2 cut(s) 394, 569
Bme1390I CCNGG 2 cut(s) 267, 300
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 2 cut(s) 425, 880
BmiI GGNNCC 2 cut(s) 304, 595
BmrFI CCNGG 2 cut(s) 267, 300
BmsI GCATC 4 cut(s) 204, 268, 736, 1021
BpiI GAAGAC 3 cut(s) 99, 189, 780
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpmI CTGGAG 3 cut(s) 57, 249, 282
Bpu10I CCTNAGC 1 cut(s) 192
BpuEI CTTGAG 3 cut(s) 108, 154, 179
BsaI GGTCTC 1 cut(s) 830
BsaJI CCNNGG 4 cut(s) 52, 134, 239, 485
BsaXI ACNNNNNCTCC 2 cut(s) 135, 165
Bsc4I CCNNNNNNNGG 5 cut(s) 453, 660, 706, 742, 847
Bse1I ACTGG 1 cut(s) 74
BseBI CCWGG 2 cut(s) 267, 300
BseDI CCNNGG 4 cut(s) 52, 134, 239, 485
BseGI GGATG 6 cut(s) 130, 311, 495, 669, 721, 751
BseLI CCNNNNNNNGG 5 cut(s) 453, 660, 706, 742, 847
BseMII CTCAG 2 cut(s) 111, 206
BseNI ACTGG 1 cut(s) 74
BseRI GAGGAG 2 cut(s) 295, 510
BseXI GCAGC 2 cut(s) 379, 579
Bsh1236I CGCG 2 cut(s) 433, 487
BshFI GGCC 1 cut(s) 881
BsiHKAI GWGCWC 1 cut(s) 193
BslFI GGGAC 2 cut(s) 70, 448
BslI CCNNNNNNNGG 5 cut(s) 453, 660, 706, 742, 847
BsmAI GTCTC 2 cut(s) 236, 830
BsmFI GGGAC 2 cut(s) 70, 448
BsmI GAATGC 3 cut(s) 49, 857, 896
BsnI GGCC 1 cut(s) 881
Bso31I GGTCTC 1 cut(s) 830
Bsp1286I GDGCHC 2 cut(s) 193, 598
Bsp143I GATC 4 cut(s) 293, 335, 413, 630
Bsp19I CCATGG 1 cut(s) 134
BspACI CCGC 3 cut(s) 485, 487, 743
BspANI GGCC 1 cut(s) 881
BspCNI CTCAG 2 cut(s) 110, 205
BspFNI CGCG 2 cut(s) 433, 487
BspLI GGNNCC 2 cut(s) 304, 595
BspMAI CTGCAG 2 cut(s) 424, 803
BspMI ACCTGC 2 cut(s) 427, 969
BspPI GGATC 2 cut(s) 301, 421
BspTI CTTAAG 1 cut(s) 655
BspTNI GGTCTC 1 cut(s) 830
BsrI ACTGG 1 cut(s) 74
BssECI CCNNGG 4 cut(s) 52, 134, 239, 485
BssMI GATC 4 cut(s) 293, 335, 413, 630
BssT1I CCWWGG 3 cut(s) 52, 134, 239
Bst2UI CCWGG 2 cut(s) 267, 300
Bst4CI ACNGT 1 cut(s) 822
Bst6I CTCTTC 3 cut(s) 313, 488, 783
BstAFI CTTAAG 1 cut(s) 655
BstC8I GCNNGC 3 cut(s) 49, 613, 944
BstDEI CTNAG 4 cut(s) 9, 97, 192, 353
BstDSI CCRYGG 2 cut(s) 134, 485
BstEII GGTNACC 1 cut(s) 649
BstENI CCTNNNNNAGG 1 cut(s) 658
BstF5I GGATG 6 cut(s) 130, 311, 495, 669, 721, 751
BstFNI CGCG 2 cut(s) 433, 487
BstHHI GCGC 2 cut(s) 433, 896
BstKTI GATC 4 cut(s) 296, 338, 416, 633
BstMAI GTCTC 2 cut(s) 236, 830
BstMBI GATC 4 cut(s) 293, 335, 413, 630
BstMWI GCNNNNNNNGC 2 cut(s) 428, 439
BstNI CCWGG 2 cut(s) 267, 300
BstNSI RCATGY 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 649
BstSCI CCNGG 2 cut(s) 265, 298
BstSFI CTRYAG 2 cut(s) 420, 799
BstUI CGCG 2 cut(s) 433, 487
BstV1I GCAGC 2 cut(s) 379, 579
BstV2I GAAGAC 3 cut(s) 99, 189, 780
BstX2I RGATCY 2 cut(s) 293, 335
BstYI RGATCY 2 cut(s) 293, 335
BsuI GTATCC 1 cut(s) 1034
BsuRI GGCC 1 cut(s) 881
BtgI CCRYGG 2 cut(s) 134, 485
BtsCI GGATG 6 cut(s) 130, 311, 495, 669, 721, 751
BveI ACCTGC 2 cut(s) 427, 969
Cac8I GCNNGC 3 cut(s) 49, 613, 944
CfoI GCGC 2 cut(s) 433, 896
Cfr13I GGNCC 2 cut(s) 425, 880
Cfr42I CCGCGG 1 cut(s) 488
CviAII CATG 3 cut(s) 20, 135, 551
DdeI CTNAG 4 cut(s) 9, 97, 192, 353
DpnI GATC 4 cut(s) 295, 337, 415, 632
DpnII GATC 4 cut(s) 293, 335, 413, 630
DraIII CACNNNGTG 1 cut(s) 554
Eam1104I CTCTTC 3 cut(s) 313, 488, 783
EarI CTCTTC 3 cut(s) 313, 488, 783
Ecl136II GAGCTC 1 cut(s) 191
Eco130I CCWWGG 3 cut(s) 52, 134, 239
Eco24I GRGCYC 2 cut(s) 193, 598
Eco31I GGTCTC 1 cut(s) 830
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 191
Eco57I CTGAAG 3 cut(s) 132, 360, 804
Eco91I GGTNACC 1 cut(s) 649
EcoICRI GAGCTC 1 cut(s) 191
EcoNI CCTNNNNNAGG 1 cut(s) 658
EcoO65I GGTNACC 1 cut(s) 649
EcoRII CCWGG 2 cut(s) 265, 298
EcoT14I CCWWGG 3 cut(s) 52, 134, 239
EcoT38I GRGCYC 2 cut(s) 193, 598
ErhI CCWWGG 3 cut(s) 52, 134, 239
FaeI CATG 3 cut(s) 23, 138, 554
FaiI YATR 7 cut(s) 21, 136, 449, 552, 779, 899, 940
FalI AAGNNNNNCTT 4 cut(s) 638, 670, 855, 887
FaqI GGGAC 2 cut(s) 70, 448
FatI CATG 3 cut(s) 19, 134, 550
Fnu4HI GCNGC 2 cut(s) 393, 568
FokI GGATG 6 cut(s) 117, 298, 502, 676, 728, 758
FriOI GRGCYC 2 cut(s) 193, 598
Fsp4HI GCNGC 2 cut(s) 393, 568
FspBI CTAG 4 cut(s) 240, 539, 812, 1066
GlaI GCGC 2 cut(s) 432, 895
GluI GCNGC 2 cut(s) 393, 568
GsuI CTGGAG 3 cut(s) 57, 249, 282
HaeIII GGCC 1 cut(s) 881
HhaI GCGC 2 cut(s) 433, 896
Hin1II CATG 3 cut(s) 23, 138, 554
Hin6I GCGC 2 cut(s) 431, 894
HinP1I GCGC 2 cut(s) 431, 894
HindIII AAGCTT 1 cut(s) 355
HinfI GANTC 4 cut(s) 95, 364, 542, 668
HphI GGTGA 6 cut(s) 38, 226, 408, 782, 845, 878
Hpy166II GTNNAC 2 cut(s) 480, 869
Hpy188I TCNGA 6 cut(s) 348, 379, 409, 630, 784, 995
Hpy188III TCNNGA 5 cut(s) 171, 194, 361, 385, 887
Hpy8I GTNNAC 2 cut(s) 480, 869
HpyAV CCTTC 7 cut(s) 382, 404, 453, 699, 700, 704, 821
HpyCH4III ACNGT 1 cut(s) 822
HpyCH4V TGCA 9 cut(s) 47, 206, 281, 422, 527, 801, 855, 861, 942
HpyF10VI GCNNNNNNNGC 2 cut(s) 428, 439
HpyF3I CTNAG 4 cut(s) 9, 97, 192, 353
Hsp92II CATG 3 cut(s) 23, 138, 554
HspAI GCGC 2 cut(s) 431, 894
KspI CCGCGG 1 cut(s) 488
Kzo9I GATC 4 cut(s) 293, 335, 413, 630
LmnI GCTCC 4 cut(s) 188, 308, 593, 620
Lsp1109I GCAGC 2 cut(s) 379, 579
LweI GCATC 4 cut(s) 204, 268, 736, 1021
MaeI CTAG 4 cut(s) 240, 539, 812, 1066
MaeIII GTNAC 4 cut(s) 514, 649, 920, 967
MalI GATC 4 cut(s) 295, 337, 415, 632
MboI GATC 4 cut(s) 293, 335, 413, 630
MfeI CAATTG 1 cut(s) 60
MflI RGATCY 2 cut(s) 293, 335
MhlI GDGCHC 2 cut(s) 193, 598
MluCI AATT 7 cut(s) 60, 250, 285, 403, 577, 684, 804
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 1 cut(s) 245
MroXI GAANNNNTTC 1 cut(s) 373
MseI TTAA 2 cut(s) 255, 656
MslI CAYNNNNRTG 1 cut(s) 24
MspA1I CMGCKG 2 cut(s) 487, 743
MspCI CTTAAG 1 cut(s) 655
MspR9I CCNGG 2 cut(s) 267, 300
MunI CAATTG 1 cut(s) 60
Mva1269I GAATGC 3 cut(s) 49, 857, 896
MvaI CCWGG 2 cut(s) 267, 300
MvnI CGCG 2 cut(s) 433, 487
MwoI GCNNNNNNNGC 2 cut(s) 428, 439
NcoI CCATGG 1 cut(s) 134
NdeII GATC 4 cut(s) 293, 335, 413, 630
NlaIII CATG 3 cut(s) 23, 138, 554
NlaIV GGNNCC 2 cut(s) 304, 595
NmuCI GTSAC 1 cut(s) 967
NspI RCATGY 1 cut(s) 23
PaqCI CACCTGC 1 cut(s) 427
PctI GAATGC 3 cut(s) 49, 857, 896
PdmI GAANNNNTTC 1 cut(s) 373
PfeI GAWTC 3 cut(s) 364, 542, 668
PkrI GCNGC 2 cut(s) 394, 569
PleI GAGTC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 89
Psp124BI GAGCTC 1 cut(s) 193
Psp6I CCWGG 2 cut(s) 265, 298
PspEI GGTNACC 1 cut(s) 649
PspGI CCWGG 2 cut(s) 265, 298
PspN4I GGNNCC 2 cut(s) 304, 595
PspPI GGNCC 2 cut(s) 425, 880
PstI CTGCAG 2 cut(s) 424, 803
PsuI RGATCY 2 cut(s) 293, 335
RseI CAYNNNNRTG 1 cut(s) 24
SacI GAGCTC 1 cut(s) 193
SacII CCGCGG 1 cut(s) 488
SaqAI TTAA 2 cut(s) 255, 656
SatI GCNGC 2 cut(s) 393, 568
Sau3AI GATC 4 cut(s) 293, 335, 413, 630
Sau96I GGNCC 2 cut(s) 425, 880
SbfI CCTGCAGG 1 cut(s) 424
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 267, 300
SdaI CCTGCAGG 1 cut(s) 424
SduI GDGCHC 2 cut(s) 193, 598
SfaNI GCATC 4 cut(s) 204, 268, 736, 1021
SfcI CTRYAG 2 cut(s) 420, 799
Sfr303I CCGCGG 1 cut(s) 488
SgrBI CCGCGG 1 cut(s) 488
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 1 cut(s) 24
SmlI CTYRAG 4 cut(s) 123, 158, 169, 655
SmoI CTYRAG 4 cut(s) 123, 158, 169, 655
Sse8387I CCTGCAGG 1 cut(s) 424
Sse9I AATT 7 cut(s) 60, 250, 285, 403, 577, 684, 804
SsiI CCGC 3 cut(s) 485, 487, 743
SspMI CTAG 4 cut(s) 240, 539, 812, 1066
SstI GAGCTC 1 cut(s) 193
StyD4I CCNGG 2 cut(s) 265, 298
StyI CCWWGG 3 cut(s) 52, 134, 239
TaaI ACNGT 1 cut(s) 822
TaqI TCGA 5 cut(s) 367, 384, 601, 725, 1000
TasI AATT 7 cut(s) 60, 250, 285, 403, 577, 684, 804
TfiI GAWTC 3 cut(s) 364, 542, 668
Tru1I TTAA 2 cut(s) 255, 656
Tru9I TTAA 2 cut(s) 255, 656
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 2 cut(s) 392, 567
Tsp45I GTSAC 1 cut(s) 967
TspDTI ATGAA 4 cut(s) 119, 300, 506, 719
TspGWI ACGGA 2 cut(s) 201, 668
Vha464I CTTAAG 1 cut(s) 655
VpaK11BI GGWCC 1 cut(s) 425
XagI CCTNNNNNAGG 1 cut(s) 658
XapI RAATTY 4 cut(s) 250, 403, 577, 804
XceI RCATGY 1 cut(s) 23
XmaJI CCTAGG 1 cut(s) 239
XmnI GAANNNNTTC 1 cut(s) 373
XspI CTAG 4 cut(s) 240, 539, 812, 1066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.