AT5G61540

Asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008429
Physical Location & Seq
Forward (+)
24744853 .. 24747542
2690 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61540.3

Sequence Viewer

Length: 1080 bp
ATGGCGAGAAGCGATGTTTTGATTTTTGTTTCTACTCTTCTTCTGTTTCTGTCTCTGCTTACGGTAGCTGATGCGGAATTGGTGAAATCTGACAAGTTTCCGGTAGTGGTGAGTACTTGGCCTTTCCTTGAAGCTGTTAGAGCTGCTTGGAGAGCAGTTGATAATGGGAGCTCTGCAGTGGAAGCTGTTGTTGAAGGTTGTTCTGCTTGTGAAGAACTTCGTTGTGACGGTACAGTTGGGCCTGGAGGAAGTCCGGATGAGAATGGTGAGACCATGATTGATGCTTTAGTCATGGATGGGGTGACTATGGAGGTTGGAGCTGTTGCTGCAATGCGGTATGTTAAAGACGGTATAAGAGCTGCACATCTAGTGATGAAATACTCTCAACACACGTTGCTTGCCGGAGAAGGAGCATCAGCATTTGCAATCTCCATGGGTCTCCCCGGACCCATGAACCTAAGCTCCCCTGAATCTGTAAAGAAGTGGTCGGACTGGAAAGAGAATCAGTGTCAACCCAATTTCAGGAAGAATGTAGTCCCCGCAAATGACTGTGGACCTTACAAGCCAAACAATAGTGCTATGAATGTTTTTGTAGACAAAAGCACTGAATCTTGCGAGATGGGGGCGATTGAGTATAAACCTCCTCTTGTTGGTCCTCACAATCACGATACGATATCGATGGCTGTCATTGACAGAATGGGGCATATAGCTGTTGGGACATCGACCAATGGAGCCACATATAAAATTCCTGGAAGGGTTGGTGATGGGCCTATCGTCGGTTCATCAGCCTACGCTGATGATGAAGTAGGTGGATGTGGTGCAACTGGAGATGGTGACACCATGATGCGCTTCCTCCCTTGTTACCAAGTTGTGGAGAGCATGAGACAAGGAATGAAACCAGAAGAAGCTGCTAAAGATGCAATCTCGAGAATCGCAAGGAAGTTTCCAGATTTCGTGGGAGCAGTTGTGGCTGTTGACAAGAACGGATCTCACGCAGGAGCTTGCTATGGCTGGACGTTTCAGTACTCAGTCCAAAACCCTGACATGAACGATGTTCAAGTCTTTACAGTTCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

359

Amino Acids

38.25

Weight (kDa)

4.87

Isoelectric Point (pI)

20.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 1 - 233 6.8e-56 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 871
AccI GTMKAC 1 cut(s) 594
AccIII TCCGGA 1 cut(s) 253
AciI CCGC 3 cut(s) 74, 334, 540
AclWI GGATC 1 cut(s) 994
AcsI RAATTY 1 cut(s) 744
AfaI GTAC 3 cut(s) 115, 232, 1025
AfiI CCNNNNNNNGG 4 cut(s) 522, 650, 776, 871
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 3 cut(s) 131, 194, 1058
AjnI CCWGG 2 cut(s) 241, 748
AloI GAACNNNNNNTCC 2 cut(s) 446, 478
Alw21I GWGCWC 1 cut(s) 173
Alw26I GTCTC 4 cut(s) 57, 263, 443, 877
AlwI GGATC 1 cut(s) 994
Ama87I CYCGRG 1 cut(s) 925
Aor13HI TCCGGA 1 cut(s) 253
AoxI GGCC 3 cut(s) 119, 239, 767
ApeKI GCWGC 4 cut(s) 143, 326, 359, 908
ApoI RAATTY 1 cut(s) 744
Asp700I GAANNNNTTC 1 cut(s) 216
AspLEI GCGC 1 cut(s) 849
AspS9I GGNCC 5 cut(s) 239, 446, 554, 653, 767
AsuC2I CCSGG 1 cut(s) 444
AsuHPI GGTGA 6 cut(s) 94, 121, 278, 313, 773, 845
AvaI CYCGRG 1 cut(s) 925
AvaII GGWCC 3 cut(s) 446, 554, 653
BanII GRGCYC 1 cut(s) 173
Bbv12I GWGCWC 1 cut(s) 173
BbvI GCAGC 4 cut(s) 130, 313, 346, 895
BccI CCATC 5 cut(s) 290, 613, 673, 758, 824
BciT130I CCWGG 2 cut(s) 243, 750
BcnI CCSGG 1 cut(s) 444
BcoDI GTCTC 4 cut(s) 57, 263, 443, 877
BfaI CTAG 1 cut(s) 368
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 4 cut(s) 144, 327, 360, 909
BlsI GCNGC 4 cut(s) 145, 328, 361, 910
BmcAI AGTACT 2 cut(s) 115, 1025
Bme1390I CCNGG 3 cut(s) 243, 444, 750
Bme18I GGWCC 3 cut(s) 446, 554, 653
BmeT110I CYCGRG 1 cut(s) 925
BmgT120I GGNCC 5 cut(s) 239, 446, 554, 653, 767
BmiI GGNNCC 2 cut(s) 448, 733
BmrFI CCNGG 3 cut(s) 243, 444, 750
BmsI GCATC 5 cut(s) 61, 271, 422, 834, 907
BpmI CTGGAG 2 cut(s) 264, 846
Bpu10I CCTNAGC 1 cut(s) 458
BpuMI CCSGG 1 cut(s) 444
Bsa29I ATCGAT 1 cut(s) 677
BsaI GGTCTC 2 cut(s) 263, 443
BsaJI CCNNGG 2 cut(s) 432, 442
BsaWI WCCGGW 2 cut(s) 100, 253
BsaXI ACNNNNNCTCC 4 cut(s) 446, 476, 951, 981
Bsc4I CCNNNNNNNGG 4 cut(s) 522, 650, 776, 871
Bse1I ACTGG 2 cut(s) 497, 829
Bse3DI GCAATG 1 cut(s) 336
BseAI TCCGGA 1 cut(s) 253
BseBI CCWGG 2 cut(s) 243, 750
BseCI ATCGAT 1 cut(s) 677
BseDI CCNNGG 2 cut(s) 432, 442
BseGI GGATG 3 cut(s) 262, 301, 818
BseLI CCNNNNNNNGG 4 cut(s) 522, 650, 776, 871
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 1 cut(s) 1041
BseNI ACTGG 2 cut(s) 497, 829
BseRI GAGGAG 1 cut(s) 633
BseXI GCAGC 4 cut(s) 130, 313, 346, 895
BsgI GTGCAG 1 cut(s) 345
BshFI GGCC 3 cut(s) 121, 241, 769
BshVI ATCGAT 1 cut(s) 677
BsiHKAI GWGCWC 1 cut(s) 173
BsiHKCI CYCGRG 1 cut(s) 925
BsiSI CCGG 4 cut(s) 101, 254, 402, 444
BslFI GGGAC 2 cut(s) 521, 730
BslI CCNNNNNNNGG 4 cut(s) 522, 650, 776, 871
BsmAI GTCTC 4 cut(s) 57, 263, 443, 877
BsmFI GGGAC 2 cut(s) 521, 730
BsnI GGCC 3 cut(s) 121, 241, 769
Bso31I GGTCTC 2 cut(s) 263, 443
BsoBI CYCGRG 1 cut(s) 925
Bsp1286I GDGCHC 1 cut(s) 173
Bsp13I TCCGGA 1 cut(s) 253
Bsp143I GATC 1 cut(s) 986
Bsp19I CCATGG 1 cut(s) 432
BspACI CCGC 3 cut(s) 74, 334, 540
BspANI GGCC 3 cut(s) 121, 241, 769
BspCNI CTCAG 1 cut(s) 1040
BspDI ATCGAT 1 cut(s) 677
BspEI TCCGGA 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 448, 733
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 1 cut(s) 994
BspTNI GGTCTC 2 cut(s) 263, 443
BsrDI GCAATG 1 cut(s) 336
BsrI ACTGG 2 cut(s) 497, 829
BssECI CCNNGG 2 cut(s) 432, 442
BssMI GATC 1 cut(s) 986
BssT1I CCWWGG 1 cut(s) 432
Bst2UI CCWGG 2 cut(s) 243, 750
Bst4CI ACNGT 6 cut(s) 64, 230, 235, 350, 551, 1069
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 399, 1003
BstDEI CTNAG 2 cut(s) 458, 1027
BstDSI CCRYGG 1 cut(s) 432
BstF5I GGATG 3 cut(s) 262, 301, 818
BstHHI GCGC 1 cut(s) 849
BstKTI GATC 1 cut(s) 989
BstMAI GTCTC 4 cut(s) 57, 263, 443, 877
BstMBI GATC 1 cut(s) 986
BstMWI GCNNNNNNNGC 6 cut(s) 140, 152, 182, 326, 917, 968
BstNI CCWGG 2 cut(s) 243, 750
BstSCI CCNGG 3 cut(s) 241, 442, 748
BstSFI CTRYAG 1 cut(s) 174
BstV1I GCAGC 4 cut(s) 130, 313, 346, 895
BstX2I RGATCY 1 cut(s) 986
BstYI RGATCY 1 cut(s) 986
Bsu15I ATCGAT 1 cut(s) 677
BsuRI GGCC 3 cut(s) 121, 241, 769
BsuTUI ATCGAT 1 cut(s) 677
BtgI CCRYGG 1 cut(s) 432
BtgZI GCGATG 1 cut(s) 27
BtsCI GGATG 3 cut(s) 262, 301, 818
BtsI GCAGTG 1 cut(s) 183
BtsIMutI CAGTG 3 cut(s) 183, 512, 603
Cac8I GCNNGC 2 cut(s) 399, 1003
CfoI GCGC 1 cut(s) 849
Cfr13I GGNCC 5 cut(s) 239, 446, 554, 653, 767
ClaI ATCGAT 1 cut(s) 677
Csp6I GTAC 3 cut(s) 114, 231, 1024
CviAII CATG 8 cut(s) 274, 292, 433, 451, 841, 880, 1045, 1077
CviQI GTAC 3 cut(s) 114, 231, 1024
DdeI CTNAG 2 cut(s) 458, 1027
DpnI GATC 1 cut(s) 988
DpnII GATC 1 cut(s) 986
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 173
Eco31I GGTCTC 2 cut(s) 263, 443
Eco32I GATATC 1 cut(s) 675
Eco47I GGWCC 3 cut(s) 446, 554, 653
Eco53kI GAGCTC 1 cut(s) 171
Eco88I CYCGRG 1 cut(s) 925
EcoICRI GAGCTC 1 cut(s) 171
EcoRII CCWGG 2 cut(s) 241, 748
EcoRV GATATC 1 cut(s) 675
EcoT14I CCWWGG 1 cut(s) 432
EcoT38I GRGCYC 1 cut(s) 173
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 8 cut(s) 277, 295, 436, 454, 844, 883, 1048, 1080
FaqI GGGAC 2 cut(s) 521, 730
FatI CATG 8 cut(s) 273, 291, 432, 450, 840, 879, 1044, 1076
FauI CCCGC 1 cut(s) 547
FblI GTMKAC 1 cut(s) 594
Fnu4HI GCNGC 4 cut(s) 144, 327, 360, 909
FokI GGATG 3 cut(s) 269, 308, 825
FriOI GRGCYC 1 cut(s) 173
Fsp4HI GCNGC 4 cut(s) 144, 327, 360, 909
FspBI CTAG 1 cut(s) 368
GlaI GCGC 1 cut(s) 848
GluI GCNGC 4 cut(s) 144, 327, 360, 909
GsuI CTGGAG 2 cut(s) 264, 846
HaeIII GGCC 3 cut(s) 121, 241, 769
HapII CCGG 4 cut(s) 101, 254, 402, 444
HhaI GCGC 1 cut(s) 849
Hin1II CATG 8 cut(s) 277, 295, 436, 454, 844, 883, 1048, 1080
Hin6I GCGC 1 cut(s) 847
HinP1I GCGC 1 cut(s) 847
HincII GTYRAC 2 cut(s) 512, 976
HindII GTYRAC 2 cut(s) 512, 976
HinfI GANTC 4 cut(s) 470, 502, 608, 930
HpaII CCGG 4 cut(s) 101, 254, 402, 444
HphI GGTGA 6 cut(s) 94, 121, 278, 313, 773, 845
Hpy166II GTNNAC 4 cut(s) 512, 554, 595, 976
Hpy188I TCNGA 2 cut(s) 91, 490
Hpy188III TCNNGA 6 cut(s) 254, 523, 665, 925, 927, 947
Hpy8I GTNNAC 4 cut(s) 512, 554, 595, 976
Hpy99I CGWCG 1 cut(s) 779
HpyAV CCTTC 3 cut(s) 188, 401, 747
HpyCH4III ACNGT 6 cut(s) 64, 230, 235, 350, 551, 1069
HpyCH4IV ACGT 2 cut(s) 392, 1016
HpyCH4V TGCA 6 cut(s) 176, 329, 362, 425, 821, 920
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 152, 182, 326, 917, 968
HpyF3I CTNAG 2 cut(s) 458, 1027
HpySE526I ACGT 2 cut(s) 392, 1016
Hsp92II CATG 8 cut(s) 277, 295, 436, 454, 844, 883, 1048, 1080
HspAI GCGC 1 cut(s) 847
Kpn2I TCCGGA 1 cut(s) 253
Kzo9I GATC 1 cut(s) 986
LmnI GCTCC 7 cut(s) 168, 317, 410, 467, 731, 959, 998
Lsp1109I GCAGC 4 cut(s) 130, 313, 346, 895
LweI GCATC 5 cut(s) 61, 271, 422, 834, 907
MaeI CTAG 1 cut(s) 368
MaeII ACGT 2 cut(s) 392, 1016
MaeIII GTNAC 4 cut(s) 224, 301, 833, 860
MalI GATC 1 cut(s) 988
MboI GATC 1 cut(s) 986
MboII GAAGA 6 cut(s) 29, 32, 224, 538, 914, 1064
MflI RGATCY 1 cut(s) 986
MhlI GDGCHC 1 cut(s) 173
MluCI AATT 3 cut(s) 77, 517, 744
MmeI TCCRAC 2 cut(s) 295, 468
MnlI CCTC 6 cut(s) 239, 304, 651, 654, 666, 863
MroI TCCGGA 1 cut(s) 253
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 1 cut(s) 342
MslI CAYNNNNRTG 1 cut(s) 842
MspI CCGG 4 cut(s) 101, 254, 402, 444
MspR9I CCNGG 3 cut(s) 243, 444, 750
MvaI CCWGG 2 cut(s) 243, 750
MwoI GCNNNNNNNGC 6 cut(s) 140, 152, 182, 326, 917, 968
NciI CCSGG 1 cut(s) 444
NcoI CCATGG 1 cut(s) 432
NdeII GATC 1 cut(s) 986
NlaIII CATG 8 cut(s) 277, 295, 436, 454, 844, 883, 1048, 1080
NlaIV GGNNCC 2 cut(s) 448, 733
NmuCI GTSAC 3 cut(s) 224, 301, 833
PaeR7I CTCGAG 1 cut(s) 925
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 4 cut(s) 470, 502, 608, 930
PflMI CCANNNNNTGG 1 cut(s) 871
PfoI TCCNGGA 1 cut(s) 748
PkrI GCNGC 4 cut(s) 145, 328, 361, 910
Psp124BI GAGCTC 1 cut(s) 173
Psp6I CCWGG 2 cut(s) 241, 748
PspGI CCWGG 2 cut(s) 241, 748
PspN4I GGNNCC 2 cut(s) 448, 733
PspPI GGNCC 5 cut(s) 239, 446, 554, 653, 767
PstI CTGCAG 1 cut(s) 178
PsuI RGATCY 1 cut(s) 986
RsaI GTAC 3 cut(s) 115, 232, 1025
RsaNI GTAC 3 cut(s) 114, 231, 1024
RseI CAYNNNNRTG 1 cut(s) 842
SacI GAGCTC 1 cut(s) 173
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 4 cut(s) 144, 327, 360, 909
Sau3AI GATC 1 cut(s) 986
Sau96I GGNCC 5 cut(s) 239, 446, 554, 653, 767
ScaI AGTACT 2 cut(s) 115, 1025
ScrFI CCNGG 3 cut(s) 243, 444, 750
SduI GDGCHC 1 cut(s) 173
SfaNI GCATC 5 cut(s) 61, 271, 422, 834, 907
SfcI CTRYAG 1 cut(s) 174
Sfr274I CTCGAG 1 cut(s) 925
SinI GGWCC 3 cut(s) 446, 554, 653
SlaI CTCGAG 1 cut(s) 925
SmiMI CAYNNNNRTG 1 cut(s) 842
SmlI CTYRAG 1 cut(s) 925
SmoI CTYRAG 1 cut(s) 925
Sse9I AATT 3 cut(s) 77, 517, 744
SsiI CCGC 3 cut(s) 74, 334, 540
SspMI CTAG 1 cut(s) 368
SstI GAGCTC 1 cut(s) 173
StyD4I CCNGG 3 cut(s) 241, 442, 748
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 6 cut(s) 64, 230, 235, 350, 551, 1069
TaiI ACGT 2 cut(s) 395, 1019
TaqI TCGA 3 cut(s) 677, 722, 926
TasI AATT 3 cut(s) 77, 517, 744
TatI WGTACW 2 cut(s) 113, 1023
TfiI GAWTC 4 cut(s) 470, 502, 608, 930
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 3 cut(s) 183, 512, 610
TseFI GTSAC 3 cut(s) 224, 301, 833
TseI GCWGC 4 cut(s) 143, 326, 359, 908
Tsp45I GTSAC 3 cut(s) 224, 301, 833
TspDTI ATGAA 7 cut(s) 389, 467, 596, 771, 816, 908, 1061
TspGWI ACGGA 1 cut(s) 999
TspRI CASTG 3 cut(s) 183, 512, 610
Van91I CCANNNNNTGG 1 cut(s) 871
VpaK11BI GGWCC 3 cut(s) 446, 554, 653
XapI RAATTY 1 cut(s) 744
XhoI CTCGAG 1 cut(s) 925
XmiI GTMKAC 1 cut(s) 594
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 1 cut(s) 368
ZrmI AGTACT 2 cut(s) 115, 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.