MD07G1250300.v1.1

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008429
Physical Location & Seq
Reverse (-)
31901824 .. 31905047
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1250300.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGCTGCCAACCGTCTGAGCTCTCTACCAATCTTGATCCTTACAGTTTTGCTGCTTTCCTTGGTCATAGGCCACGAAGCTTCGGATTCAGGGCAGTACCCTGTGGTGGTGAGCACATGGCCGTTCGTAGAGGCTGTCAGAGCAGCATGGAGGGCTGTGGATAATGGGTTCTCCGCCGTGGATGCCGTCGTTGAGGGTTGCTCGGCTTGCGAGGAGCTCAGATGTGATGGTACAGTTGGCCCTGGTGGGAGTCCAGATGAGAACGGAGAAACTACAATCGATGCTCTGGTCATGGATGGGGTGACAATGGAGGTTGGAGCTGTTGCTGCCATGAGGTATGTAAAGGATGGAATCAAAGCTGCCAGGTTAGTGATGCAGTATACCGAACACACTATGCTTGTCGGAGAGCAAGCCTCAGCCTTTGCAATTTCAATGGGTCTTCCAGGACCAACAAACCTTAATTCATCAGAATCCATTGAGAAGTGGACCAAATGGAAAGAAAACGACTGCCAGCCTAATTTTTGGAAAGATGTTGTACCTGCTAATAGTTGTGGCCCTTATCATGCAAAGGACTCTCCAGGCCTTTCTCATGGGCTGTGTTCCAAAACCACTAATATTAACCCAAGATCATCTCCCATTGGTCGTCACAACCACGACACCATATCAATGGCAGTTTTTGATAAAATGGGGAAAATTGCTGTTGGTACCTCGACAAATGGAGCCACATTTAAGATCCCAGGGAGGGTGGGTGATGGGCCAATTGCTGGATCTTCTGCATATGCTGATGATGAAGTTGGTGCTTGTGGTGCAACCGGAGATGGTGACGTCATGATGCGTTTCCTCCCATGTTATCAAGTTGTGGAGAGTATGAGATTAGGGATGGAACCTAAACTCGCTGCCAAGGATGCGATATCACGAATCGCGAGAAAATTCCCTGGTTTTGTGGGAGCTGTTTTTGCCATCAATAAGAAGGGCGTGCATGCCGGTGCTTGCCACGGATGGACATTTCAGTACTCAGTGAGAAGCCCGGAGATGGACGATGTGAAGGTTTACACTGTCCTGCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

356

Amino Acids

37.68

Weight (kDa)

5.64

Isoelectric Point (pI)

30.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 31 - 332 4.2e-77 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 828
Acc36I ACCTGC 1 cut(s) 547
Acc65I GGTACC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 704
AccB7I CCANNNNNTGG 1 cut(s) 764
AccI GTMKAC 1 cut(s) 380
AccII CGCG 1 cut(s) 923
AciI CCGC 1 cut(s) 174
AclWI GGATC 3 cut(s) 31, 727, 775
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 1 cut(s) 929
AcyI GRCGYC 1 cut(s) 825
AfaI GTAC 5 cut(s) 98, 232, 537, 706, 1013
AfiI CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
AgsI TTSAA 1 cut(s) 432
AjnI CCWGG 6 cut(s) 241, 362, 442, 577, 736, 934
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 6 cut(s) 21, 80, 217, 320, 359, 950
AluI AGCT 6 cut(s) 21, 80, 217, 320, 359, 950
Alw21I GWGCWC 3 cut(s) 23, 116, 219
AlwI GGATC 3 cut(s) 31, 727, 775
AoxI GGCC 6 cut(s) 70, 119, 238, 553, 580, 755
ApeKI GCWGC 6 cut(s) 5, 52, 143, 326, 359, 896
ApoI RAATTY 1 cut(s) 929
Asp718I GGTACC 1 cut(s) 704
AspS9I GGNCC 5 cut(s) 239, 446, 486, 554, 755
AsuC2I CCSGG 1 cut(s) 1028
AsuHPI GGTGA 4 cut(s) 121, 313, 761, 833
AvaII GGWCC 2 cut(s) 446, 486
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 2 cut(s) 23, 219
BbsI GAAGAC 1 cut(s) 431
Bbv12I GWGCWC 3 cut(s) 23, 116, 219
BbvCI CCTCAGC 1 cut(s) 415
BbvI GCAGC 5 cut(s) 39, 155, 313, 346, 883
BccI CCATC 9 cut(s) 221, 290, 341, 746, 812, 874, 968, 993, 1027
BceAI ACGGC 4 cut(s) 106, 161, 170, 1048
BciT130I CCWGG 6 cut(s) 243, 364, 444, 579, 738, 936
BcnI CCSGG 1 cut(s) 1028
BfuAI ACCTGC 1 cut(s) 547
BisI GCNGC 6 cut(s) 6, 53, 144, 327, 360, 897
BlsI GCNGC 6 cut(s) 7, 54, 145, 328, 361, 898
BmcAI AGTACT 1 cut(s) 1013
Bme1390I CCNGG 7 cut(s) 243, 364, 444, 579, 738, 936, 1028
Bme18I GGWCC 2 cut(s) 446, 486
BmgT120I GGNCC 5 cut(s) 239, 446, 486, 554, 755
BmiI GGNNCC 3 cut(s) 706, 721, 885
BmrFI CCNGG 7 cut(s) 243, 364, 444, 579, 738, 936, 1028
BmsI GCATC 5 cut(s) 172, 271, 363, 822, 895
BpiI GAAGAC 1 cut(s) 431
BplI GAGNNNNNCTC 4 cut(s) 185, 217, 398, 430
BpmI CTGGAG 1 cut(s) 561
Bpu10I CCTNAGC 1 cut(s) 415
BpuMI CCSGG 1 cut(s) 1028
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 1 cut(s) 825
BsaJI CCNNGG 8 cut(s) 60, 177, 241, 736, 737, 900, 934, 994
BsaWI WCCGGW 1 cut(s) 812
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
Bse118I RCCGGY 1 cut(s) 983
BseBI CCWGG 6 cut(s) 243, 364, 444, 579, 738, 936
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 8 cut(s) 60, 177, 241, 736, 737, 900, 934, 994
BseGI GGATG 6 cut(s) 187, 301, 352, 885, 910, 1004
BseLI CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
BseMII CTCAG 4 cut(s) 8, 232, 429, 1029
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 5 cut(s) 39, 155, 313, 346, 883
Bsh1236I CGCG 1 cut(s) 923
BshFI GGCC 6 cut(s) 72, 121, 240, 555, 582, 757
BshNI GGYRCC 1 cut(s) 704
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 3 cut(s) 23, 116, 219
BsiSI CCGG 3 cut(s) 813, 984, 1028
BslI CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
BsnI GGCC 6 cut(s) 72, 121, 240, 555, 582, 757
Bsp1286I GDGCHC 3 cut(s) 23, 116, 219
Bsp143I GATC 4 cut(s) 36, 626, 732, 767
Bsp68I TCGCGA 1 cut(s) 923
BspACI CCGC 1 cut(s) 174
BspANI GGCC 6 cut(s) 72, 121, 240, 555, 582, 757
BspCNI CTCAG 4 cut(s) 9, 231, 428, 1028
BspDI ATCGAT 1 cut(s) 279
BspFNI CGCG 1 cut(s) 923
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 3 cut(s) 706, 721, 885
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 3 cut(s) 31, 727, 775
BspT107I GGYRCC 1 cut(s) 704
BsrFI RCCGGY 1 cut(s) 983
BssAI RCCGGY 1 cut(s) 983
BssECI CCNNGG 8 cut(s) 60, 177, 241, 736, 737, 900, 934, 994
BssMI GATC 4 cut(s) 36, 626, 732, 767
BssNAI GTATAC 1 cut(s) 381
BssNI GRCGYC 1 cut(s) 825
BssT1I CCWWGG 2 cut(s) 60, 900
Bst1107I GTATAC 1 cut(s) 381
Bst2UI CCWGG 6 cut(s) 243, 364, 444, 579, 738, 936
Bst4CI ACNGT 4 cut(s) 14, 46, 235, 1057
BstACI GRCGYC 1 cut(s) 825
BstC8I GCNNGC 6 cut(s) 208, 411, 512, 977, 981, 991
BstDEI CTNAG 4 cut(s) 17, 218, 415, 1015
BstDSI CCRYGG 2 cut(s) 177, 994
BstF5I GGATG 6 cut(s) 187, 301, 352, 885, 910, 1004
BstFNI CGCG 1 cut(s) 923
BstKTI GATC 4 cut(s) 39, 629, 735, 770
BstMBI GATC 4 cut(s) 36, 626, 732, 767
BstMWI GCNNNNNNNGC 8 cut(s) 140, 152, 182, 207, 326, 806, 905, 956
BstNI CCWGG 6 cut(s) 243, 364, 444, 579, 738, 936
BstNSI RCATGY 1 cut(s) 983
BstSCI CCNGG 7 cut(s) 241, 362, 442, 577, 736, 934, 1026
BstUI CGCG 1 cut(s) 923
BstV1I GCAGC 5 cut(s) 39, 155, 313, 346, 883
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 2 cut(s) 732, 767
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 2 cut(s) 732, 767
BstZ17I GTATAC 1 cut(s) 381
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 6 cut(s) 72, 121, 240, 555, 582, 757
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 2 cut(s) 177, 994
BtsCI GGATG 6 cut(s) 187, 301, 352, 885, 910, 1004
BtsIMutI CAGTG 2 cut(s) 1023, 1053
BtuMI TCGCGA 1 cut(s) 923
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 6 cut(s) 208, 411, 512, 977, 981, 991
CciI TCATGA 1 cut(s) 828
Cfr10I RCCGGY 1 cut(s) 983
Cfr13I GGNCC 5 cut(s) 239, 446, 486, 554, 755
ClaI ATCGAT 1 cut(s) 279
Csp6I GTAC 5 cut(s) 97, 231, 536, 705, 1012
CviAII CATG 9 cut(s) 117, 147, 292, 331, 563, 590, 829, 846, 980
CviQI GTAC 5 cut(s) 97, 231, 536, 705, 1012
DdeI CTNAG 4 cut(s) 17, 218, 415, 1015
DpnI GATC 4 cut(s) 38, 628, 734, 769
DpnII GATC 4 cut(s) 36, 626, 732, 767
EaeI YGGCCR 1 cut(s) 119
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 2 cut(s) 21, 217
Eco130I CCWWGG 2 cut(s) 60, 900
Eco147I AGGCCT 1 cut(s) 582
Eco24I GRGCYC 2 cut(s) 23, 219
Eco32I GATATC 1 cut(s) 912
Eco47I GGWCC 2 cut(s) 446, 486
Eco53kI GAGCTC 2 cut(s) 21, 217
EcoICRI GAGCTC 2 cut(s) 21, 217
EcoRII CCWGG 6 cut(s) 241, 362, 442, 577, 736, 934
EcoRV GATATC 1 cut(s) 912
EcoT14I CCWWGG 2 cut(s) 60, 900
EcoT38I GRGCYC 2 cut(s) 23, 219
ErhI CCWWGG 2 cut(s) 60, 900
FaeI CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
FatI CATG 9 cut(s) 116, 146, 291, 330, 562, 589, 828, 845, 979
FauNDI CATATG 1 cut(s) 778
FblI GTMKAC 1 cut(s) 380
Fnu4HI GCNGC 6 cut(s) 6, 53, 144, 327, 360, 897
FokI GGATG 6 cut(s) 194, 308, 359, 892, 917, 1011
FriOI GRGCYC 2 cut(s) 23, 219
Fsp4HI GCNGC 6 cut(s) 6, 53, 144, 327, 360, 897
GluI GCNGC 6 cut(s) 6, 53, 144, 327, 360, 897
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 6 cut(s) 72, 121, 240, 555, 582, 757
HapII CCGG 3 cut(s) 813, 984, 1028
Hin1I GRCGYC 1 cut(s) 825
Hin1II CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 6 cut(s) 86, 250, 351, 470, 572, 918
HpaII CCGG 3 cut(s) 813, 984, 1028
HphI GGTGA 4 cut(s) 121, 313, 761, 833
Hpy166II GTNNAC 3 cut(s) 381, 486, 1051
Hpy188I TCNGA 6 cut(s) 18, 85, 140, 221, 404, 469
Hpy188III TCNNGA 5 cut(s) 34, 254, 829, 915, 922
Hpy8I GTNNAC 3 cut(s) 381, 486, 1051
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 964, 1039
HpyCH4III ACNGT 4 cut(s) 14, 46, 235, 1057
HpyCH4IV ACGT 1 cut(s) 825
HpyCH4V TGCA 6 cut(s) 376, 425, 566, 776, 809, 979
HpyF10VI GCNNNNNNNGC 8 cut(s) 140, 152, 182, 207, 326, 806, 905, 956
HpyF3I CTNAG 4 cut(s) 17, 218, 415, 1015
HpySE526I ACGT 1 cut(s) 825
Hsp92I GRCGYC 1 cut(s) 825
Hsp92II CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
KpnI GGTACC 1 cut(s) 708
Kzo9I GATC 4 cut(s) 36, 626, 732, 767
LmnI GCTCC 4 cut(s) 214, 317, 719, 947
Lsp1109I GCAGC 5 cut(s) 39, 155, 313, 346, 883
LweI GCATC 5 cut(s) 172, 271, 363, 822, 895
MaeII ACGT 1 cut(s) 825
MaeIII GTNAC 3 cut(s) 301, 644, 821
MalI GATC 4 cut(s) 38, 628, 734, 769
MboI GATC 4 cut(s) 36, 626, 732, 767
MboII GAAGA 2 cut(s) 431, 762
MfeI CAATTG 1 cut(s) 759
MflI RGATCY 2 cut(s) 732, 767
MhlI GDGCHC 3 cut(s) 23, 116, 219
MluCI AATT 6 cut(s) 426, 460, 517, 693, 759, 929
MlyI GAGTC 2 cut(s) 259, 566
MmeI TCCRAC 2 cut(s) 295, 382
MseI TTAA 3 cut(s) 459, 618, 729
MslI CAYNNNNRTG 2 cut(s) 665, 984
MspI CCGG 3 cut(s) 813, 984, 1028
MspR9I CCNGG 7 cut(s) 243, 364, 444, 579, 738, 936, 1028
MunI CAATTG 1 cut(s) 759
MvaI CCWGG 6 cut(s) 243, 364, 444, 579, 738, 936
MvnI CGCG 1 cut(s) 923
MwoI GCNNNNNNNGC 8 cut(s) 140, 152, 182, 207, 326, 806, 905, 956
NciI CCSGG 1 cut(s) 1028
NdeI CATATG 1 cut(s) 778
NdeII GATC 4 cut(s) 36, 626, 732, 767
NlaIII CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
NlaIV GGNNCC 3 cut(s) 706, 721, 885
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 3 cut(s) 301, 644, 821
NruI TCGCGA 1 cut(s) 923
NspI RCATGY 1 cut(s) 983
PaeI GCATGC 1 cut(s) 983
PagI TCATGA 1 cut(s) 828
PasI CCCWGGG 1 cut(s) 737
PceI AGGCCT 1 cut(s) 582
PfeI GAWTC 4 cut(s) 86, 351, 470, 918
PflMI CCANNNNNTGG 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 6 cut(s) 7, 54, 145, 328, 361, 898
PleI GAGTC 2 cut(s) 258, 566
PpsI GAGTC 2 cut(s) 258, 566
Psp124BI GAGCTC 2 cut(s) 23, 219
Psp6I CCWGG 6 cut(s) 241, 362, 442, 577, 736, 934
PspGI CCWGG 6 cut(s) 241, 362, 442, 577, 736, 934
PspN4I GGNNCC 3 cut(s) 706, 721, 885
PspPI GGNCC 5 cut(s) 239, 446, 486, 554, 755
PsuI RGATCY 2 cut(s) 732, 767
RruI TCGCGA 1 cut(s) 923
RsaI GTAC 5 cut(s) 98, 232, 537, 706, 1013
RsaNI GTAC 5 cut(s) 97, 231, 536, 705, 1012
RseI CAYNNNNRTG 2 cut(s) 665, 984
SacI GAGCTC 2 cut(s) 23, 219
SaqAI TTAA 3 cut(s) 459, 618, 729
SatI GCNGC 6 cut(s) 6, 53, 144, 327, 360, 897
Sau3AI GATC 4 cut(s) 36, 626, 732, 767
Sau96I GGNCC 5 cut(s) 239, 446, 486, 554, 755
ScaI AGTACT 1 cut(s) 1013
SchI GAGTC 2 cut(s) 259, 566
ScrFI CCNGG 7 cut(s) 243, 364, 444, 579, 738, 936, 1028
SduI GDGCHC 3 cut(s) 23, 116, 219
SfaNI GCATC 5 cut(s) 172, 271, 363, 822, 895
SinI GGWCC 2 cut(s) 446, 486
SmiMI CAYNNNNRTG 2 cut(s) 665, 984
SphI GCATGC 1 cut(s) 983
Sse9I AATT 6 cut(s) 426, 460, 517, 693, 759, 929
SseBI AGGCCT 1 cut(s) 582
SsiI CCGC 1 cut(s) 174
SspI AATATT 1 cut(s) 616
SstI GAGCTC 2 cut(s) 23, 219
StuI AGGCCT 1 cut(s) 582
StyD4I CCNGG 7 cut(s) 241, 362, 442, 577, 736, 934, 1026
StyI CCWWGG 2 cut(s) 60, 900
TaaI ACNGT 4 cut(s) 14, 46, 235, 1057
TaiI ACGT 1 cut(s) 828
TaqI TCGA 2 cut(s) 279, 710
TasI AATT 6 cut(s) 426, 460, 517, 693, 759, 929
TatI WGTACW 1 cut(s) 1011
TfiI GAWTC 4 cut(s) 86, 351, 470, 918
Tru1I TTAA 3 cut(s) 459, 618, 729
Tru9I TTAA 3 cut(s) 459, 618, 729
TscAI CASTG 2 cut(s) 1023, 1060
TseFI GTSAC 3 cut(s) 301, 644, 821
TseI GCWGC 6 cut(s) 5, 52, 143, 326, 359, 896
Tsp45I GTSAC 3 cut(s) 301, 644, 821
TspDTI ATGAA 2 cut(s) 453, 804
TspGWI ACGGA 2 cut(s) 279, 1011
TspRI CASTG 2 cut(s) 1023, 1060
Van91I CCANNNNNTGG 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 446, 486
XapI RAATTY 1 cut(s) 929
XceI RCATGY 1 cut(s) 983
XmiI GTMKAC 1 cut(s) 380
ZraI GACGTC 1 cut(s) 826
ZrmI AGTACT 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.