Rw1G035180

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008429
Physical Location & Seq
Forward (+)
63015552 .. 63018448
2897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035180.1

Sequence Viewer

Length: 1068 bp
ATGGCTACCAAAACTCTGAGCTCTACCTGTTTCTTGACCCTTACATTTTTACTTCTCTTCCTGGTAAATACGACATCACAGAATTTAGTTTCACAGATAAAGCTATCTGCTTTACTCACTTGGCCTTTCGTAGAGGCTGTCAGAGCTGCTTGGAGGGCTGTTGATAATGGGCTTTCTGCAGTTGATGCAGTTGTTGACGGCTGTTCGGCTTGTGAGGAACTCAGATGTGATGGTACAGTTGGGCCTGGTGGGAGTCCAGATGAGAATGGAGGGACAACAATTGATGCTCTGGTCATGGATGGGGTGACAATGGAGGTTGGAGCTGTTGCTGCCATGAGGTATGTCAAAGATGGAATCAAAGCTGCTAGGTTAGTTATGCAACATACCGAACACACTCTGTTAGTTGGAGAGCAAGCCTCAGTGTTTGCTATTTCAATGGGTCTTCCAGGACCAACAAACCTTAGTTCATCAGAGTCGATGGAGAAGTGGATTAAATGGAAAGAAAATCACTGCCAACCTAACTTTTGGAAAGACGTTGTACCTAAAGGTGGATGTGGCCCTTATCATGCAAAGGCCTACTATGATGAGACATGTTCAAATACCACTCCAGTAGGCAATATTAGATCCAGTTCTCATATTGGTCTTCACAACCATGACACCATATCAATGGCAGTTATTGATAAAATGGGTCACATTGCTGTTGGTACCTCGACTAATGGAGCCACATTCAAGATCCCAGGGAGGGTGGGTGATGGACCTATTGCAGGATCTTCTTCATACGCTGATGATGAAGTGGGTGGTTGTGGTGCAACTGGAGATGGTGACATCATGATGCGTTTCCTTCCATGTTATCAAGTTGTGGAGAGTATGAGATTAGGGATGGAGCCTAAGCTTGCTGCCAAGGATGCAATATCACGCATTGCACGAAAATTCCCTGATTTTGTGGGAGCTGTTTTTGCAATTAATAAGAACGGCGTGCATGCTGGTGCTTGCCATGGCTGGACATTTCAGTACTCGGTGAGAAACCCAGACATGGATGATGTGAAGGTTTACACGGTATTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

355

Amino Acids

37.88

Weight (kDa)

5.79

Isoelectric Point (pI)

24.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 37 - 332 4.7e-74 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 704
AclWI GGATC 3 cut(s) 618, 727, 775
AcsI RAATTY 2 cut(s) 82, 929
AfaI GTAC 4 cut(s) 235, 540, 706, 1013
AfiI CCNNNNNNNGG 4 cut(s) 548, 742, 764, 1033
AflIII ACRYGT 1 cut(s) 590
AgsI TTSAA 3 cut(s) 435, 597, 730
AjnI CCWGG 4 cut(s) 60, 244, 445, 736
AluBI AGCT 7 cut(s) 21, 103, 146, 323, 362, 892, 950
AluI AGCT 7 cut(s) 21, 103, 146, 323, 362, 892, 950
Alw21I GWGCWC 1 cut(s) 23
Alw26I GTCTC 1 cut(s) 581
AlwI GGATC 3 cut(s) 618, 727, 775
AoxI GGCC 4 cut(s) 122, 242, 556, 573
ApeKI GCWGC 4 cut(s) 146, 329, 362, 896
ApoI RAATTY 2 cut(s) 82, 929
AseI ATTAAT 1 cut(s) 963
Asp718I GGTACC 1 cut(s) 704
AspS9I GGNCC 4 cut(s) 242, 449, 557, 755
AsuHPI GGTGA 4 cut(s) 316, 761, 833, 1030
AvaII GGWCC 2 cut(s) 449, 755
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 1 cut(s) 23
BbsI GAAGAC 2 cut(s) 434, 635
Bbv12I GWGCWC 1 cut(s) 23
BbvI GCAGC 4 cut(s) 133, 316, 349, 883
BccI CCATC 7 cut(s) 224, 293, 344, 472, 746, 812, 874
BceAI ACGGC 2 cut(s) 214, 988
BciT130I CCWGG 4 cut(s) 62, 246, 447, 738
BcoDI GTCTC 1 cut(s) 581
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 1 cut(s) 177
BisI GCNGC 4 cut(s) 147, 330, 363, 897
BlsI GCNGC 4 cut(s) 148, 331, 364, 898
BmcAI AGTACT 1 cut(s) 1013
Bme1390I CCNGG 4 cut(s) 62, 246, 447, 738
Bme18I GGWCC 2 cut(s) 449, 755
BmgT120I GGNCC 4 cut(s) 242, 449, 557, 755
BmiI GGNNCC 3 cut(s) 706, 721, 885
BmrFI CCNGG 4 cut(s) 62, 246, 447, 738
BmsI GCATC 4 cut(s) 175, 274, 822, 895
BpiI GAAGAC 2 cut(s) 434, 635
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 2 cut(s) 591, 834
Bpu10I CCTNAGC 1 cut(s) 888
BsaJI CCNNGG 4 cut(s) 736, 737, 900, 994
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 4 cut(s) 548, 742, 764, 1033
Bse1I ACTGG 3 cut(s) 608, 627, 817
Bse3DI GCAATG 2 cut(s) 693, 918
BseBI CCWGG 4 cut(s) 62, 246, 447, 738
BseDI CCNNGG 4 cut(s) 736, 737, 900, 994
BseGI GGATG 5 cut(s) 304, 557, 885, 910, 1042
BseLI CCNNNNNNNGG 4 cut(s) 548, 742, 764, 1033
BseMI GCAATG 2 cut(s) 693, 918
BseMII CTCAG 3 cut(s) 8, 235, 432
BseNI ACTGG 3 cut(s) 608, 627, 817
BseXI GCAGC 4 cut(s) 133, 316, 349, 883
BshFI GGCC 4 cut(s) 124, 244, 558, 575
BshNI GGYRCC 1 cut(s) 704
BsiHKAI GWGCWC 1 cut(s) 23
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 4 cut(s) 548, 742, 764, 1033
BsmAI GTCTC 1 cut(s) 581
BsmFI GGGAC 1 cut(s) 286
BsnI GGCC 4 cut(s) 124, 244, 558, 575
Bsp1286I GDGCHC 1 cut(s) 23
Bsp143I GATC 3 cut(s) 623, 732, 767
Bsp19I CCATGG 1 cut(s) 994
BspANI GGCC 4 cut(s) 124, 244, 558, 575
BspCNI CTCAG 3 cut(s) 9, 234, 431
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 3 cut(s) 706, 721, 885
BspMAI CTGCAG 1 cut(s) 181
BspPI GGATC 3 cut(s) 618, 727, 775
BspT107I GGYRCC 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 693, 918
BsrI ACTGG 3 cut(s) 608, 627, 817
BssECI CCNNGG 4 cut(s) 736, 737, 900, 994
BssMI GATC 3 cut(s) 623, 732, 767
BssT1I CCWWGG 2 cut(s) 900, 994
Bst2UI CCWGG 4 cut(s) 62, 246, 447, 738
Bst4CI ACNGT 2 cut(s) 238, 1057
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 5 cut(s) 414, 894, 977, 981, 991
BstDEI CTNAG 5 cut(s) 17, 221, 418, 461, 888
BstDSI CCRYGG 1 cut(s) 994
BstENI CCTNNNNNAGG 1 cut(s) 762
BstF5I GGATG 5 cut(s) 304, 557, 885, 910, 1042
BstKTI GATC 3 cut(s) 626, 735, 770
BstMAI GTCTC 1 cut(s) 581
BstMBI GATC 3 cut(s) 623, 732, 767
BstMWI GCNNNNNNNGC 6 cut(s) 143, 155, 185, 329, 905, 956
BstNI CCWGG 4 cut(s) 62, 246, 447, 738
BstNSI RCATGY 2 cut(s) 594, 983
BstSCI CCNGG 4 cut(s) 60, 244, 445, 736
BstSFI CTRYAG 1 cut(s) 177
BstV1I GCAGC 4 cut(s) 133, 316, 349, 883
BstV2I GAAGAC 2 cut(s) 434, 635
BstX2I RGATCY 3 cut(s) 623, 732, 767
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 3 cut(s) 623, 732, 767
BsuRI GGCC 4 cut(s) 124, 244, 558, 575
BtgI CCRYGG 1 cut(s) 994
BtsCI GGATG 5 cut(s) 304, 557, 885, 910, 1042
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 2 cut(s) 426, 508
Cac8I GCNNGC 5 cut(s) 414, 894, 977, 981, 991
CciI TCATGA 1 cut(s) 828
Cfr13I GGNCC 4 cut(s) 242, 449, 557, 755
Csp6I GTAC 4 cut(s) 234, 539, 705, 1012
CviQI GTAC 4 cut(s) 234, 539, 705, 1012
DdeI CTNAG 5 cut(s) 17, 221, 418, 461, 888
DpnI GATC 3 cut(s) 625, 734, 769
DpnII GATC 3 cut(s) 623, 732, 767
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 900, 994
Eco147I AGGCCT 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 23
Eco47I GGWCC 2 cut(s) 449, 755
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoNI CCTNNNNNAGG 1 cut(s) 762
EcoRII CCWGG 4 cut(s) 60, 244, 445, 736
EcoT14I CCWWGG 2 cut(s) 900, 994
EcoT38I GRGCYC 1 cut(s) 23
ErhI CCWWGG 2 cut(s) 900, 994
FaqI GGGAC 1 cut(s) 286
Fnu4HI GCNGC 4 cut(s) 147, 330, 363, 897
FokI GGATG 5 cut(s) 311, 564, 892, 917, 1049
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 4 cut(s) 147, 330, 363, 897
FspBI CTAG 1 cut(s) 366
GluI GCNGC 4 cut(s) 147, 330, 363, 897
GsuI CTGGAG 2 cut(s) 591, 834
HaeIII GGCC 4 cut(s) 124, 244, 558, 575
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HindIII AAGCTT 1 cut(s) 890
HinfI GANTC 3 cut(s) 253, 354, 473
HphI GGTGA 4 cut(s) 316, 761, 833, 1030
Hpy166II GTNNAC 2 cut(s) 196, 1051
Hpy188I TCNGA 4 cut(s) 18, 143, 224, 472
Hpy188III TCNNGA 4 cut(s) 34, 257, 730, 829
Hpy8I GTNNAC 2 cut(s) 196, 1051
HpyAV CCTTC 2 cut(s) 851, 1039
HpyCH4III ACNGT 2 cut(s) 238, 1057
HpyCH4IV ACGT 1 cut(s) 534
HpyF10VI GCNNNNNNNGC 6 cut(s) 143, 155, 185, 329, 905, 956
HpyF3I CTNAG 5 cut(s) 17, 221, 418, 461, 888
HpySE526I ACGT 1 cut(s) 534
KpnI GGTACC 1 cut(s) 708
Kzo9I GATC 3 cut(s) 623, 732, 767
LmnI GCTCC 4 cut(s) 320, 719, 883, 947
Lsp1109I GCAGC 4 cut(s) 133, 316, 349, 883
LweI GCATC 4 cut(s) 175, 274, 822, 895
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 534
MaeIII GTNAC 3 cut(s) 304, 689, 821
MalI GATC 3 cut(s) 625, 734, 769
MboI GATC 3 cut(s) 623, 732, 767
MboII GAAGA 5 cut(s) 49, 434, 635, 762, 765
MfeI CAATTG 1 cut(s) 279
MflI RGATCY 3 cut(s) 623, 732, 767
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 4 cut(s) 82, 279, 929, 960
MlyI GAGTC 2 cut(s) 262, 482
MmeI TCCRAC 2 cut(s) 298, 385
MnlI CCTC 9 cut(s) 127, 147, 208, 263, 307, 330, 427, 718, 735
MseI TTAA 2 cut(s) 492, 963
MslI CAYNNNNRTG 4 cut(s) 651, 665, 830, 984
MspR9I CCNGG 4 cut(s) 62, 246, 447, 738
MunI CAATTG 1 cut(s) 279
MvaI CCWGG 4 cut(s) 62, 246, 447, 738
MwoI GCNNNNNNNGC 6 cut(s) 143, 155, 185, 329, 905, 956
NcoI CCATGG 1 cut(s) 994
NdeII GATC 3 cut(s) 623, 732, 767
NlaIV GGNNCC 3 cut(s) 706, 721, 885
NmuCI GTSAC 3 cut(s) 304, 689, 821
NspI RCATGY 2 cut(s) 594, 983
PaeI GCATGC 1 cut(s) 983
PagI TCATGA 1 cut(s) 828
PasI CCCWGGG 1 cut(s) 737
PceI AGGCCT 1 cut(s) 575
PciI ACATGT 1 cut(s) 590
PcsI WCGNNNNNNNCGW 1 cut(s) 922
PfeI GAWTC 1 cut(s) 354
PfoI TCCNGGA 1 cut(s) 445
PkrI GCNGC 4 cut(s) 148, 331, 364, 898
PleI GAGTC 2 cut(s) 261, 481
PpsI GAGTC 2 cut(s) 261, 481
PscI ACATGT 1 cut(s) 590
PshBI ATTAAT 1 cut(s) 963
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 4 cut(s) 60, 244, 445, 736
PspGI CCWGG 4 cut(s) 60, 244, 445, 736
PspN4I GGNNCC 3 cut(s) 706, 721, 885
PspPI GGNCC 4 cut(s) 242, 449, 557, 755
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 3 cut(s) 623, 732, 767
RsaI GTAC 4 cut(s) 235, 540, 706, 1013
RsaNI GTAC 4 cut(s) 234, 539, 705, 1012
RseI CAYNNNNRTG 4 cut(s) 651, 665, 830, 984
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 492, 963
SatI GCNGC 4 cut(s) 147, 330, 363, 897
Sau3AI GATC 3 cut(s) 623, 732, 767
Sau96I GGNCC 4 cut(s) 242, 449, 557, 755
ScaI AGTACT 1 cut(s) 1013
SchI GAGTC 2 cut(s) 262, 482
ScrFI CCNGG 4 cut(s) 62, 246, 447, 738
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 4 cut(s) 175, 274, 822, 895
SfcI CTRYAG 1 cut(s) 177
SinI GGWCC 2 cut(s) 449, 755
SmiMI CAYNNNNRTG 4 cut(s) 651, 665, 830, 984
SphI GCATGC 1 cut(s) 983
Sse9I AATT 4 cut(s) 82, 279, 929, 960
SseBI AGGCCT 1 cut(s) 575
SspI AATATT 1 cut(s) 619
SspMI CTAG 1 cut(s) 366
SstI GAGCTC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 575
StyD4I CCNGG 4 cut(s) 60, 244, 445, 736
StyI CCWWGG 2 cut(s) 900, 994
TaaI ACNGT 2 cut(s) 238, 1057
TaiI ACGT 1 cut(s) 537
TaqI TCGA 2 cut(s) 476, 710
TasI AATT 4 cut(s) 82, 279, 929, 960
TatI WGTACW 1 cut(s) 1011
TfiI GAWTC 1 cut(s) 354
Tru1I TTAA 2 cut(s) 492, 963
Tru9I TTAA 2 cut(s) 492, 963
TscAI CASTG 2 cut(s) 426, 515
TseFI GTSAC 3 cut(s) 304, 689, 821
TseI GCWGC 4 cut(s) 146, 329, 362, 896
Tsp45I GTSAC 3 cut(s) 304, 689, 821
TspDTI ATGAA 3 cut(s) 456, 765, 804
TspRI CASTG 2 cut(s) 426, 515
VpaK11BI GGWCC 2 cut(s) 449, 755
VspI ATTAAT 1 cut(s) 963
XagI CCTNNNNNAGG 1 cut(s) 762
XapI RAATTY 2 cut(s) 82, 929
XceI RCATGY 2 cut(s) 594, 983
XspI CTAG 1 cut(s) 366
ZrmI AGTACT 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.