Prupe.2G273700_v2.0.a1

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0008429
Physical Location & Seq
Reverse (-)
27645199 .. 27648901
3703 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G273700.2

Sequence Viewer

Length: 1110 bp
ATGAAAGACCTTCACTCTTTGCTGCCCCACTCGATGGCTGCCACCAATCTGAACTCCGCATCTATCTTTCTCCTTACATTTGCATTACTCTTCATGGTCATGGGACACGAAGCTTCGAATTCCGGGCAATACCCTGTGGTGGTGAGCACATGGCCATTCTTAGAGGCTGTGAGAGCAGCTTGGAGGGTTGTTGATAATGGGTTCTCTGCGGTGGATGCCGTTGTTGAGGGTTGTTCAGCTTGCGAGGAACTCAGATGTGATGGTACAGTTGGGCCTGGTGGGAGTCCAGATGAGAATGGAGAAACTACAATCGATGCTCTGGTCATGGATGGGGTGACAATGGAGGTTGGAGCTGTTGCTGCCATGAGGTATATAAAGGATGGAATCAAAGCTGCTAGGTTAGTGATGCAATATACCGAACACACTTTGCTTGTCGGAGAGCAAGCCGCAGCTTTTGCTATTTCTATGGGTCTTCCAGGACCAACAAACCTTAGTTCATCGGAGTCTATGGATAAGTGGACCAAATGGAAAGAAAACCACTGCCAGCCTAATTTTTGGAAAGATGTTGTACCTGTAGATAGGTGTGGCCCTTATCATGCAAAGGACTTCCTGGGCTCTTCTCATGGGACATGTTCCAAAACCACTCTGATGGAAAATATTAAATCAAGATCATCTCATGTTGGTCGTCACAACCATGACACCATATCAATGGCAGTTTTTGATAAAATGGGGAACATCGCTGTTGGTACCTCAACAAATGGAGCCACATTTAAGATCCCAGGGAGGGTGGGTGATGGACCAATTGCAGGATCTTCTGCATATGCTGATGACAAAGTTGGTGCTTGTGGTGCAACTGGAGATGGTGACATCATGATGCGTTTCCTTCCATGTTATCAAGTTGTGGAGAGTATGAGATTGGGGATGGAACCTAATCTTGCCGCGAAGGATGCAATATCACGAATTGCGAGAAAATTCCCTGATTTTGTAGGAGCTGTGTTTGCCGTCAATAAGAAGGGCGTGCATGCTGGTGCTTGCCATGGATGGACGTTTCAGTACTCAGTAAGAAGCCCGGAAATGGATGATGTAAAGGTTTACACCGTGGTGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

370

Amino Acids

39.57

Weight (kDa)

5.94

Isoelectric Point (pI)

23.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 746
AccB1I GGYRCC 2 cut(s) 746, 1102
AccB7I CCANNNNNTGG 1 cut(s) 34
AccII CGCG 1 cut(s) 941
AciI CCGC 4 cut(s) 57, 209, 447, 939
AclWI GGATC 2 cut(s) 769, 817
AcoI YGGCCR 1 cut(s) 152
AcsI RAATTY 2 cut(s) 118, 971
AfaI GTAC 4 cut(s) 265, 570, 748, 1055
AfiI CCNNNNNNNGG 5 cut(s) 34, 139, 784, 806, 1075
AflIII ACRYGT 1 cut(s) 629
AjnI CCWGG 4 cut(s) 274, 475, 609, 778
AleI CACNNNNGTG 1 cut(s) 1100
AluBI AGCT 7 cut(s) 113, 179, 239, 353, 392, 452, 992
AluI AGCT 7 cut(s) 113, 179, 239, 353, 392, 452, 992
Alw21I GWGCWC 1 cut(s) 149
AlwI GGATC 2 cut(s) 769, 817
AoxI GGCC 3 cut(s) 152, 272, 586
ApeKI GCWGC 6 cut(s) 22, 38, 176, 359, 392, 449
ApoI RAATTY 2 cut(s) 118, 971
Asp718I GGTACC 1 cut(s) 746
AspS9I GGNCC 5 cut(s) 272, 479, 519, 587, 797
AsuC2I CCSGG 2 cut(s) 124, 1070
AsuHPI GGTGA 4 cut(s) 154, 346, 803, 875
AsuII TTCGAA 1 cut(s) 116
AvaII GGWCC 3 cut(s) 479, 519, 797
BaeGI GKGCMC 1 cut(s) 1107
BalI TGGCCA 1 cut(s) 154
BanI GGYRCC 2 cut(s) 746, 1102
BanII GRGCYC 1 cut(s) 617
BbsI GAAGAC 1 cut(s) 464
Bbv12I GWGCWC 1 cut(s) 149
BbvI GCAGC 6 cut(s) 9, 25, 188, 346, 379, 461
BccI CCATC 9 cut(s) 28, 254, 323, 374, 643, 788, 854, 916, 1035
BceAI ACGGC 2 cut(s) 203, 986
BciT130I CCWGG 4 cut(s) 276, 477, 611, 780
BcnI CCSGG 2 cut(s) 124, 1070
BfaI CTAG 1 cut(s) 396
BfmI CTRYAG 1 cut(s) 573
BisI GCNGC 8 cut(s) 23, 39, 177, 360, 393, 447, 450, 939
BlsI GCNGC 8 cut(s) 24, 40, 178, 361, 394, 448, 451, 940
BmcAI AGTACT 1 cut(s) 1055
Bme1390I CCNGG 6 cut(s) 124, 276, 477, 611, 780, 1070
Bme18I GGWCC 3 cut(s) 479, 519, 797
BmgT120I GGNCC 5 cut(s) 272, 479, 519, 587, 797
BmiI GGNNCC 4 cut(s) 748, 763, 927, 1104
BmrFI CCNGG 6 cut(s) 124, 276, 477, 611, 780, 1070
BmsI GCATC 6 cut(s) 68, 205, 304, 396, 864, 937
BpiI GAAGAC 1 cut(s) 464
BpmI CTGGAG 1 cut(s) 876
Bpu14I TTCGAA 1 cut(s) 116
BpuMI CCSGG 2 cut(s) 124, 1070
Bsa29I ATCGAT 1 cut(s) 312
BsaJI CCNNGG 5 cut(s) 610, 778, 779, 1036, 1098
BsaXI ACNNNNNCTCC 2 cut(s) 775, 805
Bsc4I CCNNNNNNNGG 5 cut(s) 34, 139, 784, 806, 1075
Bse1I ACTGG 1 cut(s) 859
BseBI CCWGG 4 cut(s) 276, 477, 611, 780
BseCI ATCGAT 1 cut(s) 312
BseDI CCNNGG 5 cut(s) 610, 778, 779, 1036, 1098
BseGI GGATG 7 cut(s) 220, 334, 385, 927, 952, 1046, 1084
BseLI CCNNNNNNNGG 5 cut(s) 34, 139, 784, 806, 1075
BseMII CTCAG 2 cut(s) 265, 1071
BseNI ACTGG 1 cut(s) 859
BseSI GKGCMC 1 cut(s) 1107
BseXI GCAGC 6 cut(s) 9, 25, 188, 346, 379, 461
Bsh1236I CGCG 1 cut(s) 941
BshFI GGCC 3 cut(s) 154, 274, 588
BshNI GGYRCC 2 cut(s) 746, 1102
BshVI ATCGAT 1 cut(s) 312
BsiHKAI GWGCWC 1 cut(s) 149
BsiSI CCGG 2 cut(s) 123, 1070
BslFI GGGAC 2 cut(s) 117, 640
BslI CCNNNNNNNGG 5 cut(s) 34, 139, 784, 806, 1075
BsmFI GGGAC 2 cut(s) 117, 640
BsnI GGCC 3 cut(s) 154, 274, 588
Bsp119I TTCGAA 1 cut(s) 116
Bsp1286I GDGCHC 3 cut(s) 149, 617, 1107
Bsp143I GATC 3 cut(s) 668, 774, 809
Bsp19I CCATGG 1 cut(s) 1036
BspACI CCGC 4 cut(s) 57, 209, 447, 939
BspANI GGCC 3 cut(s) 154, 274, 588
BspCNI CTCAG 2 cut(s) 264, 1070
BspDI ATCGAT 1 cut(s) 312
BspFNI CGCG 1 cut(s) 941
BspHI TCATGA 1 cut(s) 870
BspLI GGNNCC 4 cut(s) 748, 763, 927, 1104
BspPI GGATC 2 cut(s) 769, 817
BspQI GCTCTTC 1 cut(s) 622
BspT104I TTCGAA 1 cut(s) 116
BspT107I GGYRCC 2 cut(s) 746, 1102
BsrI ACTGG 1 cut(s) 859
BssECI CCNNGG 5 cut(s) 610, 778, 779, 1036, 1098
BssMI GATC 3 cut(s) 668, 774, 809
BssT1I CCWWGG 1 cut(s) 1036
Bst2UI CCWGG 4 cut(s) 276, 477, 611, 780
Bst4CI ACNGT 2 cut(s) 268, 1099
Bst6I CTCTTC 2 cut(s) 95, 622
BstAPI GCANNNNNTGC 1 cut(s) 455
BstBI TTCGAA 1 cut(s) 116
BstC8I GCNNGC 6 cut(s) 241, 444, 545, 1019, 1023, 1033
BstDEI CTNAG 4 cut(s) 160, 251, 491, 1057
BstDSI CCRYGG 2 cut(s) 1036, 1098
BstF5I GGATG 7 cut(s) 220, 334, 385, 927, 952, 1046, 1084
BstFNI CGCG 1 cut(s) 941
BstKTI GATC 3 cut(s) 671, 777, 812
BstMBI GATC 3 cut(s) 668, 774, 809
BstMWI GCNNNNNNNGC 7 cut(s) 173, 215, 359, 455, 848, 947, 998
BstNI CCWGG 4 cut(s) 276, 477, 611, 780
BstNSI RCATGY 2 cut(s) 633, 1025
BstSCI CCNGG 6 cut(s) 122, 274, 475, 609, 778, 1068
BstSFI CTRYAG 1 cut(s) 573
BstSLI GKGCMC 1 cut(s) 1107
BstUI CGCG 1 cut(s) 941
BstV1I GCAGC 6 cut(s) 9, 25, 188, 346, 379, 461
BstV2I GAAGAC 1 cut(s) 464
BstX2I RGATCY 2 cut(s) 774, 809
BstXI CCANNNNNNTGG 2 cut(s) 649, 709
BstYI RGATCY 2 cut(s) 774, 809
Bsu15I ATCGAT 1 cut(s) 312
BsuRI GGCC 3 cut(s) 154, 274, 588
BsuTUI ATCGAT 1 cut(s) 312
BtgI CCRYGG 2 cut(s) 1036, 1098
BtgZI GCGATG 1 cut(s) 721
BtsCI GGATG 7 cut(s) 220, 334, 385, 927, 952, 1046, 1084
BtsI GCAGTG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 538
Cac8I GCNNGC 6 cut(s) 241, 444, 545, 1019, 1023, 1033
CciI TCATGA 1 cut(s) 870
Cfr13I GGNCC 5 cut(s) 272, 479, 519, 587, 797
ClaI ATCGAT 1 cut(s) 312
Csp6I GTAC 4 cut(s) 264, 569, 747, 1054
CviQI GTAC 4 cut(s) 264, 569, 747, 1054
DdeI CTNAG 4 cut(s) 160, 251, 491, 1057
DpnI GATC 3 cut(s) 670, 776, 811
DpnII GATC 3 cut(s) 668, 774, 809
EaeI YGGCCR 1 cut(s) 152
Eam1104I CTCTTC 2 cut(s) 95, 622
EarI CTCTTC 2 cut(s) 95, 622
Eco130I CCWWGG 1 cut(s) 1036
Eco24I GRGCYC 1 cut(s) 617
Eco47I GGWCC 3 cut(s) 479, 519, 797
EcoRI GAATTC 1 cut(s) 118
EcoRII CCWGG 4 cut(s) 274, 475, 609, 778
EcoT14I CCWWGG 1 cut(s) 1036
EcoT38I GRGCYC 1 cut(s) 617
ErhI CCWWGG 1 cut(s) 1036
FaqI GGGAC 2 cut(s) 117, 640
FauNDI CATATG 1 cut(s) 820
Fnu4HI GCNGC 8 cut(s) 23, 39, 177, 360, 393, 447, 450, 939
FokI GGATG 7 cut(s) 227, 341, 392, 934, 959, 1053, 1091
FriOI GRGCYC 1 cut(s) 617
Fsp4HI GCNGC 8 cut(s) 23, 39, 177, 360, 393, 447, 450, 939
FspBI CTAG 1 cut(s) 396
GluI GCNGC 8 cut(s) 23, 39, 177, 360, 393, 447, 450, 939
GsuI CTGGAG 1 cut(s) 876
HaeIII GGCC 3 cut(s) 154, 274, 588
HapII CCGG 2 cut(s) 123, 1070
HindIII AAGCTT 1 cut(s) 111
HinfI GANTC 3 cut(s) 283, 384, 503
HpaII CCGG 2 cut(s) 123, 1070
HphI GGTGA 4 cut(s) 154, 346, 803, 875
Hpy166II GTNNAC 2 cut(s) 519, 1093
Hpy188I TCNGA 5 cut(s) 51, 254, 437, 502, 648
Hpy188III TCNNGA 4 cut(s) 287, 666, 871, 957
Hpy8I GTNNAC 2 cut(s) 519, 1093
HpyAV CCTTC 4 cut(s) 20, 893, 937, 1006
HpyCH4III ACNGT 2 cut(s) 268, 1099
HpyCH4IV ACGT 1 cut(s) 1046
HpyCH4V TGCA 8 cut(s) 83, 409, 599, 806, 818, 851, 950, 1021
HpyF10VI GCNNNNNNNGC 7 cut(s) 173, 215, 359, 455, 848, 947, 998
HpyF3I CTNAG 4 cut(s) 160, 251, 491, 1057
HpySE526I ACGT 1 cut(s) 1046
KpnI GGTACC 1 cut(s) 750
Kzo9I GATC 3 cut(s) 668, 774, 809
LguI GCTCTTC 1 cut(s) 622
LmnI GCTCC 3 cut(s) 350, 761, 989
Lsp1109I GCAGC 6 cut(s) 9, 25, 188, 346, 379, 461
LweI GCATC 6 cut(s) 68, 205, 304, 396, 864, 937
MaeI CTAG 1 cut(s) 396
MaeII ACGT 1 cut(s) 1046
MaeIII GTNAC 3 cut(s) 334, 686, 863
MalI GATC 3 cut(s) 670, 776, 811
MboI GATC 3 cut(s) 668, 774, 809
MboII GAAGA 4 cut(s) 82, 464, 609, 804
MfeI CAATTG 1 cut(s) 801
MflI RGATCY 2 cut(s) 774, 809
MhlI GDGCHC 3 cut(s) 149, 617, 1107
MlsI TGGCCA 1 cut(s) 154
MluCI AATT 5 cut(s) 118, 550, 801, 960, 971
MluNI TGGCCA 1 cut(s) 154
MlyI GAGTC 2 cut(s) 292, 512
MmeI TCCRAC 2 cut(s) 328, 415
MnlI CCTC 8 cut(s) 157, 177, 220, 238, 337, 360, 760, 777
Mox20I TGGCCA 1 cut(s) 154
MscI TGGCCA 1 cut(s) 154
MseI TTAA 2 cut(s) 660, 771
MslI CAYNNNNRTG 7 cut(s) 98, 647, 693, 707, 872, 1026, 1100
Msp20I TGGCCA 1 cut(s) 154
MspI CCGG 2 cut(s) 123, 1070
MspR9I CCNGG 6 cut(s) 124, 276, 477, 611, 780, 1070
MunI CAATTG 1 cut(s) 801
MvaI CCWGG 4 cut(s) 276, 477, 611, 780
MvnI CGCG 1 cut(s) 941
MwoI GCNNNNNNNGC 7 cut(s) 173, 215, 359, 455, 848, 947, 998
NciI CCSGG 2 cut(s) 124, 1070
NcoI CCATGG 1 cut(s) 1036
NdeI CATATG 1 cut(s) 820
NdeII GATC 3 cut(s) 668, 774, 809
NlaIV GGNNCC 4 cut(s) 748, 763, 927, 1104
NmuCI GTSAC 3 cut(s) 334, 686, 863
NspI RCATGY 2 cut(s) 633, 1025
NspV TTCGAA 1 cut(s) 116
OliI CACNNNNGTG 1 cut(s) 1100
PaeI GCATGC 1 cut(s) 1025
PagI TCATGA 1 cut(s) 870
PasI CCCWGGG 1 cut(s) 779
PciI ACATGT 1 cut(s) 629
PciSI GCTCTTC 1 cut(s) 622
PfeI GAWTC 1 cut(s) 384
PflMI CCANNNNNTGG 1 cut(s) 34
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 8 cut(s) 24, 40, 178, 361, 394, 448, 451, 940
PleI GAGTC 2 cut(s) 291, 511
PpsI GAGTC 2 cut(s) 291, 511
PscI ACATGT 1 cut(s) 629
Psp6I CCWGG 4 cut(s) 274, 475, 609, 778
PspGI CCWGG 4 cut(s) 274, 475, 609, 778
PspN4I GGNNCC 4 cut(s) 748, 763, 927, 1104
PspPI GGNCC 5 cut(s) 272, 479, 519, 587, 797
PsuI RGATCY 2 cut(s) 774, 809
RsaI GTAC 4 cut(s) 265, 570, 748, 1055
RsaNI GTAC 4 cut(s) 264, 569, 747, 1054
RseI CAYNNNNRTG 7 cut(s) 98, 647, 693, 707, 872, 1026, 1100
SapI GCTCTTC 1 cut(s) 622
SaqAI TTAA 2 cut(s) 660, 771
SatI GCNGC 8 cut(s) 23, 39, 177, 360, 393, 447, 450, 939
Sau3AI GATC 3 cut(s) 668, 774, 809
Sau96I GGNCC 5 cut(s) 272, 479, 519, 587, 797
ScaI AGTACT 1 cut(s) 1055
SchI GAGTC 2 cut(s) 292, 512
ScrFI CCNGG 6 cut(s) 124, 276, 477, 611, 780, 1070
SduI GDGCHC 3 cut(s) 149, 617, 1107
SfaNI GCATC 6 cut(s) 68, 205, 304, 396, 864, 937
SfcI CTRYAG 1 cut(s) 573
SfuI TTCGAA 1 cut(s) 116
SinI GGWCC 3 cut(s) 479, 519, 797
SmiMI CAYNNNNRTG 7 cut(s) 98, 647, 693, 707, 872, 1026, 1100
SphI GCATGC 1 cut(s) 1025
Sse9I AATT 5 cut(s) 118, 550, 801, 960, 971
SsiI CCGC 4 cut(s) 57, 209, 447, 939
SspI AATATT 1 cut(s) 658
SspMI CTAG 1 cut(s) 396
StyD4I CCNGG 6 cut(s) 122, 274, 475, 609, 778, 1068
StyI CCWWGG 1 cut(s) 1036
TaaI ACNGT 2 cut(s) 268, 1099
TaiI ACGT 1 cut(s) 1049
TaqI TCGA 3 cut(s) 32, 116, 312
TasI AATT 5 cut(s) 118, 550, 801, 960, 971
TatI WGTACW 1 cut(s) 1053
TauI GCSGC 2 cut(s) 449, 941
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 2 cut(s) 660, 771
Tru9I TTAA 2 cut(s) 660, 771
TscAI CASTG 1 cut(s) 545
TseFI GTSAC 3 cut(s) 334, 686, 863
TseI GCWGC 6 cut(s) 22, 38, 176, 359, 392, 449
Tsp45I GTSAC 3 cut(s) 334, 686, 863
TspDTI ATGAA 3 cut(s) 17, 82, 486
TspRI CASTG 1 cut(s) 545
Van91I CCANNNNNTGG 1 cut(s) 34
VpaK11BI GGWCC 3 cut(s) 479, 519, 797
XapI RAATTY 2 cut(s) 118, 971
XceI RCATGY 2 cut(s) 633, 1025
XspI CTAG 1 cut(s) 396
ZrmI AGTACT 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.