pycom07g22330

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008429
Physical Location & Seq
Reverse (-)
23707327 .. 23710135
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22330.5

Sequence Viewer

Length: 1068 bp
ATGGCTGCCAACCGTCTGAGCTCTCTACCAATCTTGATCCTTACATTTTCACTGCTTTCCTTGGTCATAGGCCAGGAAGCTTCGGATTCAGGGCAGTACCCTGTGGTGGTGAGCACATGGCCCTTCGTAGAGGCTGTCAGAGCAGCATGGAGGGCTGTGGATAATGGGTTCTCCGCCGTGGATTCCGTCGTTGAGGGTTGCTCGGCTTGCGAGGAGCTCAGATGTGATGGTACAGTTGGGCCTGGTGGGAGTCCAGATGAGAACGGAGAAACTACAATCGATGCTTTGGTCATGGATGGGGTGACAATGGAGGTTGGAGCTGTTGCTGCCATGAGGTATGTAAGGGAAGGAATCAAAGCTGCTAGGTTAGTGATGCAGTATACCGAACACACTTTGCTTGTCGGAGAGAAGGCGTCAGCCTTTGCAATTTCAATGGGTCTTCCAGGACCAACAAACCTTAGTTCATCAGAATCCATTGAGAAGTGGACCAAATGGAAAGAAAACGACTGCCAGCCTAATTTTTGGAAAGATGTCGTACCTGCTAATAGTTGTGGCCCTTATCATGCAAAGGACTCTCTAGGTCTTTCTCATGGGCTGTGTTCCAAAACCACTAATATTAACCCAAGATCATCTCCCATTGGTCGTCACAACCACGACACCATATCAATGGCAGTTTTTGATAAAATGGGGAAAATTGCCGTTGGTACCTCGACAAATGGAGCCACATTTAAGATCCCAGGGAGGGTGGGTGATGGGCCAATTGCTGGATCTTCTGCATATGCTGATGATGAAGTTGGTGCTTGTGGTGCAACCGGAGATGGTGATGTCATGATGCGTTTCCTTCCATGTTATCAAGTTGTGGAGAGTATGAGATTAGGGATGGAACCTAAACTCGCTGCTAAGGATGCGATGTCACGAATAGCGAGAAAATTCCCTGGTTTTGTTGGAGCTGTTTTCGCCATCAATAAGAAGGGCGTGCATGCCGGTGCTTGCCACGGATGGACATTTCAGTACTCAGTGAGAAGCCCGGAGATGGACGATGTCAAGGTTTACACCGTGCTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

356

Amino Acids

37.74

Weight (kDa)

5.69

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 547
Acc65I GGTACC 1 cut(s) 704
AccB1I GGYRCC 1 cut(s) 704
AccB7I CCANNNNNTGG 1 cut(s) 764
AccI GTMKAC 1 cut(s) 380
AciI CCGC 1 cut(s) 174
AclWI GGATC 3 cut(s) 31, 727, 775
AcsI RAATTY 1 cut(s) 929
AcyI GRCGYC 1 cut(s) 413
AfaI GTAC 5 cut(s) 98, 232, 537, 706, 1013
AfiI CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
AgsI TTSAA 1 cut(s) 432
AjnI CCWGG 5 cut(s) 72, 241, 442, 736, 934
AloI GAACNNNNNNTCC 2 cut(s) 152, 184
AluBI AGCT 6 cut(s) 21, 80, 217, 320, 359, 950
AluI AGCT 6 cut(s) 21, 80, 217, 320, 359, 950
Alw21I GWGCWC 3 cut(s) 23, 116, 219
AlwI GGATC 3 cut(s) 31, 727, 775
AoxI GGCC 5 cut(s) 70, 119, 239, 553, 755
ApeKI GCWGC 5 cut(s) 5, 143, 326, 359, 896
ApoI RAATTY 1 cut(s) 929
Asp718I GGTACC 1 cut(s) 704
AspS9I GGNCC 6 cut(s) 120, 239, 446, 486, 554, 755
AsuC2I CCSGG 1 cut(s) 1028
AsuHPI GGTGA 4 cut(s) 121, 313, 761, 833
AvaII GGWCC 2 cut(s) 446, 486
BanI GGYRCC 1 cut(s) 704
BanII GRGCYC 2 cut(s) 23, 219
BbsI GAAGAC 1 cut(s) 431
Bbv12I GWGCWC 3 cut(s) 23, 116, 219
BbvI GCAGC 4 cut(s) 155, 313, 346, 883
BccI CCATC 8 cut(s) 221, 290, 746, 812, 874, 968, 993, 1027
BceAI ACGGC 2 cut(s) 161, 683
BciT130I CCWGG 5 cut(s) 74, 243, 444, 738, 936
BcnI CCSGG 1 cut(s) 1028
BfaI CTAG 2 cut(s) 363, 578
BfuAI ACCTGC 1 cut(s) 547
BisI GCNGC 5 cut(s) 6, 144, 327, 360, 897
BlsI GCNGC 5 cut(s) 7, 145, 328, 361, 898
BmcAI AGTACT 1 cut(s) 1013
Bme1390I CCNGG 6 cut(s) 74, 243, 444, 738, 936, 1028
Bme18I GGWCC 2 cut(s) 446, 486
BmgT120I GGNCC 6 cut(s) 120, 239, 446, 486, 554, 755
BmiI GGNNCC 3 cut(s) 706, 721, 885
BmrFI CCNGG 6 cut(s) 74, 243, 444, 738, 936, 1028
BmsI GCATC 4 cut(s) 271, 363, 822, 895
BpiI GAAGAC 1 cut(s) 431
BplI GAGNNNNNCTC 2 cut(s) 185, 217
Bpu10I CCTNAGC 1 cut(s) 900
BpuMI CCSGG 1 cut(s) 1028
Bsa29I ATCGAT 1 cut(s) 279
BsaHI GRCGYC 1 cut(s) 413
BsaJI CCNNGG 6 cut(s) 60, 177, 736, 737, 934, 994
BsaWI WCCGGW 1 cut(s) 812
BsaXI ACNNNNNCTCC 2 cut(s) 733, 763
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
Bse118I RCCGGY 1 cut(s) 983
BseBI CCWGG 5 cut(s) 74, 243, 444, 738, 936
BseCI ATCGAT 1 cut(s) 279
BseDI CCNNGG 6 cut(s) 60, 177, 736, 737, 934, 994
BseGI GGATG 4 cut(s) 301, 885, 910, 1004
BseLI CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
BseMII CTCAG 3 cut(s) 8, 232, 1029
BseRI GAGGAG 1 cut(s) 227
BseXI GCAGC 4 cut(s) 155, 313, 346, 883
BshFI GGCC 5 cut(s) 72, 121, 241, 555, 757
BshNI GGYRCC 1 cut(s) 704
BshVI ATCGAT 1 cut(s) 279
BsiHKAI GWGCWC 3 cut(s) 23, 116, 219
BsiSI CCGG 3 cut(s) 813, 984, 1028
BslI CCNNNNNNNGG 4 cut(s) 106, 742, 764, 1033
BsnI GGCC 5 cut(s) 72, 121, 241, 555, 757
Bsp1286I GDGCHC 3 cut(s) 23, 116, 219
Bsp143I GATC 4 cut(s) 36, 626, 732, 767
BspACI CCGC 1 cut(s) 174
BspANI GGCC 5 cut(s) 72, 121, 241, 555, 757
BspCNI CTCAG 3 cut(s) 9, 231, 1028
BspDI ATCGAT 1 cut(s) 279
BspHI TCATGA 1 cut(s) 828
BspLI GGNNCC 3 cut(s) 706, 721, 885
BspMI ACCTGC 1 cut(s) 547
BspPI GGATC 3 cut(s) 31, 727, 775
BspT107I GGYRCC 1 cut(s) 704
BsrFI RCCGGY 1 cut(s) 983
BssAI RCCGGY 1 cut(s) 983
BssECI CCNNGG 6 cut(s) 60, 177, 736, 737, 934, 994
BssMI GATC 4 cut(s) 36, 626, 732, 767
BssNAI GTATAC 1 cut(s) 381
BssNI GRCGYC 1 cut(s) 413
BssT1I CCWWGG 1 cut(s) 60
Bst1107I GTATAC 1 cut(s) 381
Bst2UI CCWGG 5 cut(s) 74, 243, 444, 738, 936
Bst4CI ACNGT 4 cut(s) 14, 235, 1057, 1065
BstACI GRCGYC 1 cut(s) 413
BstC8I GCNNGC 5 cut(s) 208, 512, 977, 981, 991
BstDEI CTNAG 5 cut(s) 17, 218, 458, 900, 1015
BstDSI CCRYGG 2 cut(s) 177, 994
BstF5I GGATG 4 cut(s) 301, 885, 910, 1004
BstKTI GATC 4 cut(s) 39, 629, 735, 770
BstMBI GATC 4 cut(s) 36, 626, 732, 767
BstMWI GCNNNNNNNGC 7 cut(s) 140, 152, 207, 326, 806, 905, 956
BstNI CCWGG 5 cut(s) 74, 243, 444, 738, 936
BstNSI RCATGY 1 cut(s) 983
BstSCI CCNGG 6 cut(s) 72, 241, 442, 736, 934, 1026
BstV1I GCAGC 4 cut(s) 155, 313, 346, 883
BstV2I GAAGAC 1 cut(s) 431
BstX2I RGATCY 2 cut(s) 732, 767
BstXI CCANNNNNNTGG 1 cut(s) 667
BstYI RGATCY 2 cut(s) 732, 767
BstZ17I GTATAC 1 cut(s) 381
Bsu15I ATCGAT 1 cut(s) 279
BsuRI GGCC 5 cut(s) 72, 121, 241, 555, 757
BsuTUI ATCGAT 1 cut(s) 279
BtgI CCRYGG 2 cut(s) 177, 994
BtgZI GCGATG 1 cut(s) 923
BtsCI GGATG 4 cut(s) 301, 885, 910, 1004
BtsI GCAGTG 1 cut(s) 50
BtsIMutI CAGTG 2 cut(s) 50, 1023
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 5 cut(s) 208, 512, 977, 981, 991
CciI TCATGA 1 cut(s) 828
Cfr10I RCCGGY 1 cut(s) 983
Cfr13I GGNCC 6 cut(s) 120, 239, 446, 486, 554, 755
ClaI ATCGAT 1 cut(s) 279
CseI GACGC 1 cut(s) 402
Csp6I GTAC 5 cut(s) 97, 231, 536, 705, 1012
CviAII CATG 9 cut(s) 117, 147, 292, 331, 563, 590, 829, 846, 980
CviQI GTAC 5 cut(s) 97, 231, 536, 705, 1012
DdeI CTNAG 5 cut(s) 17, 218, 458, 900, 1015
DpnI GATC 4 cut(s) 38, 628, 734, 769
DpnII GATC 4 cut(s) 36, 626, 732, 767
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 2 cut(s) 21, 217
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 2 cut(s) 23, 219
Eco47I GGWCC 2 cut(s) 446, 486
Eco53kI GAGCTC 2 cut(s) 21, 217
EcoICRI GAGCTC 2 cut(s) 21, 217
EcoRII CCWGG 5 cut(s) 72, 241, 442, 736, 934
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 2 cut(s) 23, 219
ErhI CCWWGG 1 cut(s) 60
FaeI CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
FatI CATG 9 cut(s) 116, 146, 291, 330, 562, 589, 828, 845, 979
FauNDI CATATG 1 cut(s) 778
FblI GTMKAC 1 cut(s) 380
Fnu4HI GCNGC 5 cut(s) 6, 144, 327, 360, 897
FokI GGATG 4 cut(s) 308, 892, 917, 1011
FriOI GRGCYC 2 cut(s) 23, 219
Fsp4HI GCNGC 5 cut(s) 6, 144, 327, 360, 897
FspBI CTAG 2 cut(s) 363, 578
GluI GCNGC 5 cut(s) 6, 144, 327, 360, 897
HaeIII GGCC 5 cut(s) 72, 121, 241, 555, 757
HapII CCGG 3 cut(s) 813, 984, 1028
HgaI GACGC 1 cut(s) 402
Hin1I GRCGYC 1 cut(s) 413
Hin1II CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 6 cut(s) 86, 182, 250, 351, 470, 572
HpaII CCGG 3 cut(s) 813, 984, 1028
HphI GGTGA 4 cut(s) 121, 313, 761, 833
Hpy166II GTNNAC 3 cut(s) 381, 486, 1051
Hpy188I TCNGA 6 cut(s) 18, 85, 140, 221, 404, 469
Hpy188III TCNNGA 4 cut(s) 34, 254, 829, 915
Hpy8I GTNNAC 3 cut(s) 381, 486, 1051
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 5 cut(s) 133, 341, 403, 851, 964
HpyCH4III ACNGT 4 cut(s) 14, 235, 1057, 1065
HpyCH4V TGCA 6 cut(s) 376, 425, 566, 776, 809, 979
HpyF10VI GCNNNNNNNGC 7 cut(s) 140, 152, 207, 326, 806, 905, 956
HpyF3I CTNAG 5 cut(s) 17, 218, 458, 900, 1015
Hsp92I GRCGYC 1 cut(s) 413
Hsp92II CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
KpnI GGTACC 1 cut(s) 708
Kzo9I GATC 4 cut(s) 36, 626, 732, 767
LmnI GCTCC 4 cut(s) 214, 317, 719, 947
Lsp1109I GCAGC 4 cut(s) 155, 313, 346, 883
LweI GCATC 4 cut(s) 271, 363, 822, 895
MaeI CTAG 2 cut(s) 363, 578
MaeIII GTNAC 3 cut(s) 301, 644, 912
MalI GATC 4 cut(s) 38, 628, 734, 769
MboI GATC 4 cut(s) 36, 626, 732, 767
MboII GAAGA 2 cut(s) 431, 762
MfeI CAATTG 1 cut(s) 759
MflI RGATCY 2 cut(s) 732, 767
MhlI GDGCHC 3 cut(s) 23, 116, 219
MluCI AATT 5 cut(s) 426, 517, 693, 759, 929
MlyI GAGTC 2 cut(s) 259, 566
MmeI TCCRAC 3 cut(s) 295, 382, 925
MnlI CCTC 8 cut(s) 124, 144, 187, 205, 304, 327, 718, 735
MseI TTAA 2 cut(s) 618, 729
MslI CAYNNNNRTG 2 cut(s) 665, 984
MspI CCGG 3 cut(s) 813, 984, 1028
MspR9I CCNGG 6 cut(s) 74, 243, 444, 738, 936, 1028
MunI CAATTG 1 cut(s) 759
MvaI CCWGG 5 cut(s) 74, 243, 444, 738, 936
MwoI GCNNNNNNNGC 7 cut(s) 140, 152, 207, 326, 806, 905, 956
NciI CCSGG 1 cut(s) 1028
NdeI CATATG 1 cut(s) 778
NdeII GATC 4 cut(s) 36, 626, 732, 767
NlaIII CATG 9 cut(s) 120, 150, 295, 334, 566, 593, 832, 849, 983
NlaIV GGNNCC 3 cut(s) 706, 721, 885
NmeAIII GCCGAG 1 cut(s) 182
NmuCI GTSAC 3 cut(s) 301, 644, 912
NspI RCATGY 1 cut(s) 983
PaeI GCATGC 1 cut(s) 983
PagI TCATGA 1 cut(s) 828
PasI CCCWGGG 1 cut(s) 737
PfeI GAWTC 4 cut(s) 86, 182, 351, 470
PflFI GACNNNGTC 1 cut(s) 1040
PflMI CCANNNNNTGG 1 cut(s) 764
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 5 cut(s) 7, 145, 328, 361, 898
PleI GAGTC 2 cut(s) 258, 566
PpsI GAGTC 2 cut(s) 258, 566
Psp124BI GAGCTC 2 cut(s) 23, 219
Psp6I CCWGG 5 cut(s) 72, 241, 442, 736, 934
PspGI CCWGG 5 cut(s) 72, 241, 442, 736, 934
PspN4I GGNNCC 3 cut(s) 706, 721, 885
PspPI GGNCC 6 cut(s) 120, 239, 446, 486, 554, 755
PsuI RGATCY 2 cut(s) 732, 767
PsyI GACNNNGTC 1 cut(s) 1040
RsaI GTAC 5 cut(s) 98, 232, 537, 706, 1013
RsaNI GTAC 5 cut(s) 97, 231, 536, 705, 1012
RseI CAYNNNNRTG 2 cut(s) 665, 984
SacI GAGCTC 2 cut(s) 23, 219
SaqAI TTAA 2 cut(s) 618, 729
SatI GCNGC 5 cut(s) 6, 144, 327, 360, 897
Sau3AI GATC 4 cut(s) 36, 626, 732, 767
Sau96I GGNCC 6 cut(s) 120, 239, 446, 486, 554, 755
ScaI AGTACT 1 cut(s) 1013
SchI GAGTC 2 cut(s) 259, 566
ScrFI CCNGG 6 cut(s) 74, 243, 444, 738, 936, 1028
SduI GDGCHC 3 cut(s) 23, 116, 219
SfaNI GCATC 4 cut(s) 271, 363, 822, 895
SinI GGWCC 2 cut(s) 446, 486
SmiMI CAYNNNNRTG 2 cut(s) 665, 984
SphI GCATGC 1 cut(s) 983
Sse9I AATT 5 cut(s) 426, 517, 693, 759, 929
SsiI CCGC 1 cut(s) 174
SspI AATATT 1 cut(s) 616
SspMI CTAG 2 cut(s) 363, 578
SstI GAGCTC 2 cut(s) 23, 219
StyD4I CCNGG 6 cut(s) 72, 241, 442, 736, 934, 1026
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 4 cut(s) 14, 235, 1057, 1065
TaqI TCGA 2 cut(s) 279, 710
TasI AATT 5 cut(s) 426, 517, 693, 759, 929
TatI WGTACW 1 cut(s) 1011
TfiI GAWTC 4 cut(s) 86, 182, 351, 470
Tru1I TTAA 2 cut(s) 618, 729
Tru9I TTAA 2 cut(s) 618, 729
TscAI CASTG 2 cut(s) 57, 1023
TseFI GTSAC 3 cut(s) 301, 644, 912
TseI GCWGC 5 cut(s) 5, 143, 326, 359, 896
Tsp45I GTSAC 3 cut(s) 301, 644, 912
TspDTI ATGAA 2 cut(s) 453, 804
TspGWI ACGGA 3 cut(s) 175, 279, 1011
TspRI CASTG 2 cut(s) 57, 1023
Tth111I GACNNNGTC 1 cut(s) 1040
Van91I CCANNNNNTGG 1 cut(s) 764
VpaK11BI GGWCC 2 cut(s) 446, 486
XapI RAATTY 1 cut(s) 929
XceI RCATGY 1 cut(s) 983
XmiI GTMKAC 1 cut(s) 380
XspI CTAG 2 cut(s) 363, 578
ZrmI AGTACT 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.