Rroxscaffold_4G00283940

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0008429
Physical Location & Seq
Forward (+)
5717801 .. 5720966
3166 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283940.1

Sequence Viewer

Length: 768 bp
ATGGAGGTTGGAGCTGTTGCTGCCATGAGGTATGTCAAAGATGGAATCAAAGCTGCTAGGTTAGTTATGCAACATACCGAACACACTCTGCTAGTTGGAGAGCAAGCCTCAGTGTTTGCTATTTCCATGGGTCTTCCAGGACCAACAAACCTTAGTTCATCAGAGTCGATGGAGAAGTGGATTAAATGGAAAGAAAATCACTGCCAACCTAACTTTTGGAAAGACGTTGTACCTAAAGGTGGATGTGGCCCTTATCATGCAAAGGACTTTGTAGGCCTTTATGATGAGACATGTTCAAATACCACTCCAGTAGGCAATATTAGATCCAGTTCTCATATTGGTCTTCACAACCATGACACCATATCAATGGCTGTTATTGATAAAATGGGTCACATTGCTGTTGGTACGTCGACTAATGGAGCCACATTCAAGATCCCAGGGAGGGTGGGTGATGGACCTATTGCAGGATCTTCTTCATATGCTGATGATGAAGTGGGTGGTTGTGGTGCAACTGGAGATGGTGACATCATGATGCGTTTCCTTCCATGTTATCAAGTCGTGGAGAGTATGAGATTAGGGATGGAGCCTAAGCTTGCTGCCAAGGATGCAATATCACGCATTGCACGAAAATTCCCTGATTTTGTGGGAGCTGTTTTTGCAATTAATAAAAACGGCGTGCATGCTGGTGCTTGCCATGGATGGACATTTCAGTACTCGGTGAGAAGCCCAGACATGGATGATGTGAAGGTTTACACGGTATTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

255

Amino Acids

27.42

Weight (kDa)

6.54

Isoelectric Point (pI)

26.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 1 - 232 1.1e-52 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 410
AclWI GGATC 3 cut(s) 318, 427, 475
AcsI RAATTY 1 cut(s) 629
AfaI GTAC 3 cut(s) 231, 406, 713
AfiI CCNNNNNNNGG 4 cut(s) 239, 442, 464, 733
AflIII ACRYGT 1 cut(s) 290
AgsI TTSAA 2 cut(s) 297, 430
AjnI CCWGG 2 cut(s) 136, 436
AluBI AGCT 4 cut(s) 14, 53, 592, 650
AluI AGCT 4 cut(s) 14, 53, 592, 650
Alw26I GTCTC 1 cut(s) 281
AlwI GGATC 3 cut(s) 318, 427, 475
AoxI GGCC 2 cut(s) 247, 274
ApeKI GCWGC 3 cut(s) 20, 53, 596
ApoI RAATTY 1 cut(s) 629
AseI ATTAAT 1 cut(s) 663
AspS9I GGNCC 3 cut(s) 140, 248, 455
AsuHPI GGTGA 3 cut(s) 461, 533, 730
AvaII GGWCC 2 cut(s) 140, 455
BbsI GAAGAC 2 cut(s) 125, 335
BbvI GCAGC 3 cut(s) 7, 40, 583
BccI CCATC 6 cut(s) 35, 163, 446, 512, 574, 693
BceAI ACGGC 1 cut(s) 688
BciT130I CCWGG 2 cut(s) 138, 438
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 2 cut(s) 57, 92
BisI GCNGC 3 cut(s) 21, 54, 597
BlsI GCNGC 3 cut(s) 22, 55, 598
BmcAI AGTACT 1 cut(s) 713
Bme1390I CCNGG 2 cut(s) 138, 438
Bme18I GGWCC 2 cut(s) 140, 455
BmgT120I GGNCC 3 cut(s) 140, 248, 455
BmiI GGNNCC 2 cut(s) 421, 585
BmrFI CCNGG 2 cut(s) 138, 438
BmsI GCATC 2 cut(s) 522, 595
BpiI GAAGAC 2 cut(s) 125, 335
BplI GAGNNNNNCTC 2 cut(s) 92, 124
BpmI CTGGAG 2 cut(s) 291, 534
Bpu10I CCTNAGC 1 cut(s) 588
BsaJI CCNNGG 5 cut(s) 126, 436, 437, 600, 694
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 4 cut(s) 239, 442, 464, 733
Bse1I ACTGG 3 cut(s) 308, 327, 517
Bse3DI GCAATG 2 cut(s) 393, 618
BseBI CCWGG 2 cut(s) 138, 438
BseDI CCNNGG 5 cut(s) 126, 436, 437, 600, 694
BseGI GGATG 5 cut(s) 248, 585, 610, 704, 742
BseLI CCNNNNNNNGG 4 cut(s) 239, 442, 464, 733
BseMI GCAATG 2 cut(s) 393, 618
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 3 cut(s) 308, 327, 517
BseXI GCAGC 3 cut(s) 7, 40, 583
BshFI GGCC 2 cut(s) 249, 276
BslI CCNNNNNNNGG 4 cut(s) 239, 442, 464, 733
BsmAI GTCTC 1 cut(s) 281
BsnI GGCC 2 cut(s) 249, 276
Bsp143I GATC 3 cut(s) 323, 432, 467
Bsp19I CCATGG 2 cut(s) 126, 694
BspANI GGCC 2 cut(s) 249, 276
BspCNI CTCAG 1 cut(s) 122
BspHI TCATGA 1 cut(s) 528
BspLI GGNNCC 2 cut(s) 421, 585
BspPI GGATC 3 cut(s) 318, 427, 475
BsrDI GCAATG 2 cut(s) 393, 618
BsrI ACTGG 3 cut(s) 308, 327, 517
BssECI CCNNGG 5 cut(s) 126, 436, 437, 600, 694
BssMI GATC 3 cut(s) 323, 432, 467
BssT1I CCWWGG 3 cut(s) 126, 600, 694
Bst2UI CCWGG 2 cut(s) 138, 438
Bst4CI ACNGT 1 cut(s) 757
BstC8I GCNNGC 5 cut(s) 105, 594, 677, 681, 691
BstDEI CTNAG 3 cut(s) 109, 152, 588
BstDSI CCRYGG 2 cut(s) 126, 694
BstENI CCTNNNNNAGG 1 cut(s) 462
BstF5I GGATG 5 cut(s) 248, 585, 610, 704, 742
BstKTI GATC 3 cut(s) 326, 435, 470
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 3 cut(s) 323, 432, 467
BstMWI GCNNNNNNNGC 3 cut(s) 20, 605, 656
BstNI CCWGG 2 cut(s) 138, 438
BstNSI RCATGY 2 cut(s) 294, 683
BstSCI CCNGG 2 cut(s) 136, 436
BstV1I GCAGC 3 cut(s) 7, 40, 583
BstV2I GAAGAC 2 cut(s) 125, 335
BstX2I RGATCY 3 cut(s) 323, 432, 467
BstXI CCANNNNNNTGG 1 cut(s) 367
BstYI RGATCY 3 cut(s) 323, 432, 467
BsuRI GGCC 2 cut(s) 249, 276
BtgI CCRYGG 2 cut(s) 126, 694
BtsCI GGATG 5 cut(s) 248, 585, 610, 704, 742
BtsI GCAGTG 1 cut(s) 199
BtsIMutI CAGTG 2 cut(s) 117, 199
Cac8I GCNNGC 5 cut(s) 105, 594, 677, 681, 691
CciI TCATGA 1 cut(s) 528
Cfr13I GGNCC 3 cut(s) 140, 248, 455
Csp6I GTAC 3 cut(s) 230, 405, 712
CviQI GTAC 3 cut(s) 230, 405, 712
DdeI CTNAG 3 cut(s) 109, 152, 588
DpnI GATC 3 cut(s) 325, 434, 469
DpnII GATC 3 cut(s) 323, 432, 467
Eco130I CCWWGG 3 cut(s) 126, 600, 694
Eco147I AGGCCT 1 cut(s) 276
Eco47I GGWCC 2 cut(s) 140, 455
EcoNI CCTNNNNNAGG 1 cut(s) 462
EcoRII CCWGG 2 cut(s) 136, 436
EcoT14I CCWWGG 3 cut(s) 126, 600, 694
ErhI CCWWGG 3 cut(s) 126, 600, 694
FauNDI CATATG 1 cut(s) 478
FblI GTMKAC 1 cut(s) 410
Fnu4HI GCNGC 3 cut(s) 21, 54, 597
FokI GGATG 5 cut(s) 255, 592, 617, 711, 749
Fsp4HI GCNGC 3 cut(s) 21, 54, 597
FspBI CTAG 2 cut(s) 57, 92
GluI GCNGC 3 cut(s) 21, 54, 597
GsuI CTGGAG 2 cut(s) 291, 534
HaeIII GGCC 2 cut(s) 249, 276
HincII GTYRAC 1 cut(s) 411
HindII GTYRAC 1 cut(s) 411
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 2 cut(s) 45, 164
HphI GGTGA 3 cut(s) 461, 533, 730
Hpy166II GTNNAC 2 cut(s) 411, 751
Hpy188I TCNGA 1 cut(s) 163
Hpy188III TCNNGA 2 cut(s) 430, 529
Hpy8I GTNNAC 2 cut(s) 411, 751
Hpy99I CGWCG 1 cut(s) 412
HpyAV CCTTC 2 cut(s) 551, 739
HpyCH4III ACNGT 1 cut(s) 757
HpyCH4IV ACGT 2 cut(s) 225, 407
HpyCH4V TGCA 8 cut(s) 70, 260, 464, 509, 608, 623, 659, 679
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 605, 656
HpyF3I CTNAG 3 cut(s) 109, 152, 588
HpySE526I ACGT 2 cut(s) 225, 407
Kzo9I GATC 3 cut(s) 323, 432, 467
LmnI GCTCC 4 cut(s) 11, 419, 583, 647
Lsp1109I GCAGC 3 cut(s) 7, 40, 583
LweI GCATC 2 cut(s) 522, 595
MaeI CTAG 2 cut(s) 57, 92
MaeII ACGT 2 cut(s) 225, 407
MaeIII GTNAC 2 cut(s) 389, 521
MalI GATC 3 cut(s) 325, 434, 469
MboI GATC 3 cut(s) 323, 432, 467
MboII GAAGA 4 cut(s) 125, 335, 462, 465
MflI RGATCY 3 cut(s) 323, 432, 467
MluCI AATT 2 cut(s) 629, 660
MlyI GAGTC 1 cut(s) 173
MmeI TCCRAC 1 cut(s) 76
MnlI CCTC 3 cut(s) 21, 118, 435
MseI TTAA 2 cut(s) 183, 663
MslI CAYNNNNRTG 4 cut(s) 351, 365, 530, 684
MspR9I CCNGG 2 cut(s) 138, 438
MvaI CCWGG 2 cut(s) 138, 438
MwoI GCNNNNNNNGC 3 cut(s) 20, 605, 656
NcoI CCATGG 2 cut(s) 126, 694
NdeI CATATG 1 cut(s) 478
NdeII GATC 3 cut(s) 323, 432, 467
NlaIV GGNNCC 2 cut(s) 421, 585
NmuCI GTSAC 2 cut(s) 389, 521
NspI RCATGY 2 cut(s) 294, 683
PaeI GCATGC 1 cut(s) 683
PagI TCATGA 1 cut(s) 528
PasI CCCWGGG 1 cut(s) 437
PceI AGGCCT 1 cut(s) 276
PciI ACATGT 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 622
PfeI GAWTC 1 cut(s) 45
PfoI TCCNGGA 1 cut(s) 136
PkrI GCNGC 3 cut(s) 22, 55, 598
PleI GAGTC 1 cut(s) 172
PpsI GAGTC 1 cut(s) 172
PscI ACATGT 1 cut(s) 290
PshBI ATTAAT 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 136, 436
PspGI CCWGG 2 cut(s) 136, 436
PspN4I GGNNCC 2 cut(s) 421, 585
PspPI GGNCC 3 cut(s) 140, 248, 455
PsuI RGATCY 3 cut(s) 323, 432, 467
RsaI GTAC 3 cut(s) 231, 406, 713
RsaNI GTAC 3 cut(s) 230, 405, 712
RseI CAYNNNNRTG 4 cut(s) 351, 365, 530, 684
SalI GTCGAC 1 cut(s) 409
SaqAI TTAA 2 cut(s) 183, 663
SatI GCNGC 3 cut(s) 21, 54, 597
Sau3AI GATC 3 cut(s) 323, 432, 467
Sau96I GGNCC 3 cut(s) 140, 248, 455
ScaI AGTACT 1 cut(s) 713
SchI GAGTC 1 cut(s) 173
ScrFI CCNGG 2 cut(s) 138, 438
SfaNI GCATC 2 cut(s) 522, 595
SinI GGWCC 2 cut(s) 140, 455
SmiMI CAYNNNNRTG 4 cut(s) 351, 365, 530, 684
SphI GCATGC 1 cut(s) 683
Sse9I AATT 2 cut(s) 629, 660
SseBI AGGCCT 1 cut(s) 276
SspI AATATT 1 cut(s) 319
SspMI CTAG 2 cut(s) 57, 92
StuI AGGCCT 1 cut(s) 276
StyD4I CCNGG 2 cut(s) 136, 436
StyI CCWWGG 3 cut(s) 126, 600, 694
TaaI ACNGT 1 cut(s) 757
TaiI ACGT 2 cut(s) 228, 410
TaqI TCGA 2 cut(s) 167, 410
TasI AATT 2 cut(s) 629, 660
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 2 cut(s) 183, 663
Tru9I TTAA 2 cut(s) 183, 663
TscAI CASTG 2 cut(s) 117, 206
TseFI GTSAC 2 cut(s) 389, 521
TseI GCWGC 3 cut(s) 20, 53, 596
Tsp45I GTSAC 2 cut(s) 389, 521
TspDTI ATGAA 3 cut(s) 147, 465, 504
TspRI CASTG 2 cut(s) 117, 206
VpaK11BI GGWCC 2 cut(s) 140, 455
VspI ATTAAT 1 cut(s) 663
XagI CCTNNNNNAGG 1 cut(s) 462
XapI RAATTY 1 cut(s) 629
XceI RCATGY 2 cut(s) 294, 683
XmiI GTMKAC 1 cut(s) 410
XspI CTAG 2 cut(s) 57, 92
ZrmI AGTACT 1 cut(s) 713
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.