FvH4_7g27050

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008429
Physical Location & Seq
Reverse (-)
20303794 .. 20307448
3655 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g27050.t2

Sequence Viewer

Length: 1077 bp
ATGCCTACCAGAACAACTCTGAGCTCTACCTGTTTTTTGACCCTCACAGTTTCACTACTCTTCCTGGTTACAGGGCACCAAGCTTTGAATTCTGGGCAATACCCTGTGGTGGTGAGCACTTGGCCTTTCTTAGAGGCTGTCAGAGCTGCTTGGAGGGCTGTGGATAATGGGCTCTCTGCAGTTGATGCAGTTGTCGAGGGCTGTTCTGCTTGTGAGGAACTCAGATGTGATGGTACAGTTGGGCCTGGTGGGAGTCCAGATGAGAATGGAGAGACGACAATCGATGCTCTGGTCATGGATGGGGTGACGATGGAGGTTGGAGCTGTCGCTGCCATGAGGTATGTCAAAGATGGAATCAAAGCTGCTAGATTAGTTATGCAACATACCGAACACACTCTGCTTGTCGGAGAGCAAGCCTCAGTGTTTGCCATTTCAATGGGTCTTTCAGGACCAACAAACCTCAGTTCATCAGAGTCTATGGAGAAGTGGACTAAATGGAAAGAAAATCACTGCCAACCTAACTTTTGGAAAGATGTCGTACCTGTAGGTGGATGTGGCCCTTATCATGCAAAGGACTTCGTAGGCCTTTATGATGAGACATGTTCAAACACCACTCCGGTAGGAAATATTAGATCAAGTTCTCATATTGGTCTTCACAACCATGACACCATATCAATGGCAGTTATTGATAAAATGGGTCACATTGCTGTTGGTACATCGACTAATGGAGCTACATTTAAGATCCCAGGGAGGGTGGGTGATGGACCTATTGCAGGATCTTCTTCATATGCTGATGATGAAGTGGGTGCTTGTGGTGCAACTGGAGATGGTGACATAATGATGCGTTTCCTTCCGTGTTATCAAGTTGTGGAGAGTATGAGATTGGGGATGGAGCCTAAGCTTGCTGCCAAGGATGCAATATCACGCATCGCGCGAAAATTCCCTGATTTTGTGGGAGCTGTTTTTGCAATTAATAAGAACGGCGTGCATGCTGGTGCTTGCCATGGGTGGACGTTTCAGTACTCGGTGAGAAACCCAGACATGGATGATGTGAAGGTTTACACTGTAGTGCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

359

Amino Acids

38.13

Weight (kDa)

5.43

Isoelectric Point (pI)

24.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 1 - 237 6.7e-56 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 75
AccII CGCG 2 cut(s) 932, 934
AclWI GGATC 2 cut(s) 736, 784
AcsI RAATTY 2 cut(s) 88, 938
AfaI GTAC 4 cut(s) 235, 540, 715, 1022
AfiI CCNNNNNNNGG 5 cut(s) 109, 548, 751, 773, 1042
AflIII ACRYGT 1 cut(s) 599
AgsI TTSAA 3 cut(s) 88, 435, 606
AjnI CCWGG 3 cut(s) 63, 244, 745
AleI CACNNNNGTG 1 cut(s) 1067
AluBI AGCT 8 cut(s) 24, 83, 146, 323, 362, 731, 901, 959
AluI AGCT 8 cut(s) 24, 83, 146, 323, 362, 731, 901, 959
Alw21I GWGCWC 2 cut(s) 26, 119
Alw26I GTCTC 2 cut(s) 266, 590
AlwI GGATC 2 cut(s) 736, 784
AoxI GGCC 4 cut(s) 122, 242, 556, 583
ApeKI GCWGC 4 cut(s) 146, 329, 362, 905
ApoI RAATTY 2 cut(s) 88, 938
AseI ATTAAT 1 cut(s) 972
AspLEI GCGC 1 cut(s) 934
AspS9I GGNCC 4 cut(s) 242, 449, 557, 764
AsuHPI GGTGA 5 cut(s) 124, 316, 770, 842, 1039
AvaII GGWCC 2 cut(s) 449, 764
BaeGI GKGCMC 2 cut(s) 78, 1074
BanI GGYRCC 1 cut(s) 75
BanII GRGCYC 2 cut(s) 26, 174
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 2 cut(s) 26, 119
BbvI GCAGC 4 cut(s) 133, 316, 349, 892
BccI CCATC 7 cut(s) 224, 293, 304, 344, 755, 821, 883
BceAI ACGGC 1 cut(s) 997
BciT130I CCWGG 3 cut(s) 65, 246, 747
BcoDI GTCTC 2 cut(s) 266, 590
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 3 cut(s) 177, 543, 1065
BisI GCNGC 4 cut(s) 147, 330, 363, 906
BlsI GCNGC 4 cut(s) 148, 331, 364, 907
BmcAI AGTACT 1 cut(s) 1022
Bme1390I CCNGG 3 cut(s) 65, 246, 747
Bme18I GGWCC 2 cut(s) 449, 764
BmgT120I GGNCC 4 cut(s) 242, 449, 557, 764
BmiI GGNNCC 2 cut(s) 77, 894
BmrFI CCNGG 3 cut(s) 65, 246, 747
BmsI GCATC 5 cut(s) 175, 274, 831, 904, 936
BpiI GAAGAC 1 cut(s) 644
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 897
Bsa29I ATCGAT 1 cut(s) 282
BsaJI CCNNGG 4 cut(s) 745, 746, 909, 1003
BsaWI WCCGGW 1 cut(s) 616
BsaXI ACNNNNNCTCC 2 cut(s) 742, 772
Bsc4I CCNNNNNNNGG 5 cut(s) 109, 548, 751, 773, 1042
Bse1I ACTGG 1 cut(s) 826
Bse3DI GCAATG 1 cut(s) 702
BseBI CCWGG 3 cut(s) 65, 246, 747
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 4 cut(s) 745, 746, 909, 1003
BseGI GGATG 5 cut(s) 304, 557, 894, 919, 1051
BseLI CCNNNNNNNGG 5 cut(s) 109, 548, 751, 773, 1042
BseMI GCAATG 1 cut(s) 702
BseMII CTCAG 4 cut(s) 11, 235, 432, 475
BseNI ACTGG 1 cut(s) 826
BseSI GKGCMC 2 cut(s) 78, 1074
BseXI GCAGC 4 cut(s) 133, 316, 349, 892
Bsh1236I CGCG 2 cut(s) 932, 934
BshFI GGCC 4 cut(s) 124, 244, 558, 585
BshNI GGYRCC 1 cut(s) 75
BshVI ATCGAT 1 cut(s) 282
BsiHKAI GWGCWC 2 cut(s) 26, 119
BsiSI CCGG 1 cut(s) 617
BslI CCNNNNNNNGG 5 cut(s) 109, 548, 751, 773, 1042
BsmAI GTCTC 2 cut(s) 266, 590
BsmBI CGTCTC 1 cut(s) 266
BsnI GGCC 4 cut(s) 124, 244, 558, 585
Bsp1286I GDGCHC 5 cut(s) 26, 78, 119, 174, 1074
Bsp143I GATC 3 cut(s) 632, 741, 776
Bsp19I CCATGG 1 cut(s) 1003
BspANI GGCC 4 cut(s) 124, 244, 558, 585
BspCNI CTCAG 4 cut(s) 12, 234, 431, 474
BspDI ATCGAT 1 cut(s) 282
BspFNI CGCG 2 cut(s) 932, 934
BspLI GGNNCC 2 cut(s) 77, 894
BspMAI CTGCAG 1 cut(s) 181
BspPI GGATC 2 cut(s) 736, 784
BspT107I GGYRCC 1 cut(s) 75
BsrDI GCAATG 1 cut(s) 702
BsrI ACTGG 1 cut(s) 826
BssECI CCNNGG 4 cut(s) 745, 746, 909, 1003
BssMI GATC 3 cut(s) 632, 741, 776
BssT1I CCWWGG 2 cut(s) 909, 1003
Bst2UI CCWGG 3 cut(s) 65, 246, 747
Bst4CI ACNGT 3 cut(s) 49, 238, 1066
Bst6I CTCTTC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 185
BstC8I GCNNGC 5 cut(s) 414, 903, 986, 990, 1000
BstDEI CTNAG 6 cut(s) 20, 130, 221, 418, 461, 897
BstDSI CCRYGG 1 cut(s) 1003
BstENI CCTNNNNNAGG 1 cut(s) 771
BstF5I GGATG 5 cut(s) 304, 557, 894, 919, 1051
BstFNI CGCG 2 cut(s) 932, 934
BstHHI GCGC 1 cut(s) 934
BstKTI GATC 3 cut(s) 635, 744, 779
BstMAI GTCTC 2 cut(s) 266, 590
BstMBI GATC 3 cut(s) 632, 741, 776
BstMWI GCNNNNNNNGC 7 cut(s) 143, 155, 185, 329, 815, 914, 965
BstNI CCWGG 3 cut(s) 65, 246, 747
BstNSI RCATGY 2 cut(s) 603, 992
BstSCI CCNGG 3 cut(s) 63, 244, 745
BstSFI CTRYAG 3 cut(s) 177, 543, 1065
BstSLI GKGCMC 2 cut(s) 78, 1074
BstUI CGCG 2 cut(s) 932, 934
BstV1I GCAGC 4 cut(s) 133, 316, 349, 892
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 2 cut(s) 741, 776
BstXI CCANNNNNNTGG 2 cut(s) 436, 676
BstYI RGATCY 2 cut(s) 741, 776
Bsu15I ATCGAT 1 cut(s) 282
BsuRI GGCC 4 cut(s) 124, 244, 558, 585
BsuTUI ATCGAT 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 1003
BtgZI GCGATG 1 cut(s) 913
BtsCI GGATG 5 cut(s) 304, 557, 894, 919, 1051
BtsI GCAGTG 1 cut(s) 508
BtsIMutI CAGTG 3 cut(s) 426, 508, 1062
Cac8I GCNNGC 5 cut(s) 414, 903, 986, 990, 1000
CfoI GCGC 1 cut(s) 934
Cfr13I GGNCC 4 cut(s) 242, 449, 557, 764
ClaI ATCGAT 1 cut(s) 282
Csp6I GTAC 4 cut(s) 234, 539, 714, 1021
CviAII CATG 8 cut(s) 295, 334, 566, 600, 662, 989, 1004, 1042
CviQI GTAC 4 cut(s) 234, 539, 714, 1021
DdeI CTNAG 6 cut(s) 20, 130, 221, 418, 461, 897
DpnI GATC 3 cut(s) 634, 743, 778
DpnII GATC 3 cut(s) 632, 741, 776
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 2 cut(s) 909, 1003
Eco147I AGGCCT 1 cut(s) 585
Eco24I GRGCYC 2 cut(s) 26, 174
Eco47I GGWCC 2 cut(s) 449, 764
Eco53kI GAGCTC 1 cut(s) 24
EcoICRI GAGCTC 1 cut(s) 24
EcoNI CCTNNNNNAGG 1 cut(s) 771
EcoRI GAATTC 1 cut(s) 88
EcoRII CCWGG 3 cut(s) 63, 244, 745
EcoT14I CCWWGG 2 cut(s) 909, 1003
EcoT38I GRGCYC 2 cut(s) 26, 174
ErhI CCWWGG 2 cut(s) 909, 1003
Esp3I CGTCTC 1 cut(s) 266
FaeI CATG 8 cut(s) 298, 337, 569, 603, 665, 992, 1007, 1045
FatI CATG 8 cut(s) 294, 333, 565, 599, 661, 988, 1003, 1041
FauNDI CATATG 1 cut(s) 787
Fnu4HI GCNGC 4 cut(s) 147, 330, 363, 906
FokI GGATG 5 cut(s) 311, 564, 901, 926, 1058
FriOI GRGCYC 2 cut(s) 26, 174
Fsp4HI GCNGC 4 cut(s) 147, 330, 363, 906
FspBI CTAG 1 cut(s) 366
GlaI GCGC 1 cut(s) 933
GluI GCNGC 4 cut(s) 147, 330, 363, 906
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 4 cut(s) 124, 244, 558, 585
HapII CCGG 1 cut(s) 617
HhaI GCGC 1 cut(s) 934
Hin1II CATG 8 cut(s) 298, 337, 569, 603, 665, 992, 1007, 1045
Hin6I GCGC 1 cut(s) 932
HinP1I GCGC 1 cut(s) 932
HindIII AAGCTT 2 cut(s) 81, 899
HinfI GANTC 3 cut(s) 253, 354, 473
HpaII CCGG 1 cut(s) 617
HphI GGTGA 5 cut(s) 124, 316, 770, 842, 1039
Hpy166II GTNNAC 3 cut(s) 489, 1011, 1060
Hpy188I TCNGA 5 cut(s) 21, 143, 224, 407, 472
Hpy188III TCNNGA 2 cut(s) 257, 447
Hpy8I GTNNAC 3 cut(s) 489, 1011, 1060
HpyAV CCTTC 2 cut(s) 860, 1048
HpyCH4III ACNGT 3 cut(s) 49, 238, 1066
HpyCH4IV ACGT 1 cut(s) 1013
HpyCH4V TGCA 9 cut(s) 179, 188, 379, 569, 773, 818, 917, 968, 988
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 155, 185, 329, 815, 914, 965
HpyF3I CTNAG 6 cut(s) 20, 130, 221, 418, 461, 897
HpySE526I ACGT 1 cut(s) 1013
Hsp92II CATG 8 cut(s) 298, 337, 569, 603, 665, 992, 1007, 1045
HspAI GCGC 1 cut(s) 932
Kzo9I GATC 3 cut(s) 632, 741, 776
LmnI GCTCC 4 cut(s) 320, 728, 892, 956
Lsp1109I GCAGC 4 cut(s) 133, 316, 349, 892
LweI GCATC 5 cut(s) 175, 274, 831, 904, 936
MaeI CTAG 1 cut(s) 366
MaeII ACGT 1 cut(s) 1013
MaeIII GTNAC 4 cut(s) 67, 304, 698, 830
MalI GATC 3 cut(s) 634, 743, 778
MboI GATC 3 cut(s) 632, 741, 776
MboII GAAGA 4 cut(s) 52, 644, 771, 774
MflI RGATCY 2 cut(s) 741, 776
MhlI GDGCHC 5 cut(s) 26, 78, 119, 174, 1074
MluCI AATT 3 cut(s) 88, 938, 969
MlyI GAGTC 2 cut(s) 262, 482
MmeI TCCRAC 2 cut(s) 298, 385
MseI TTAA 2 cut(s) 738, 972
MslI CAYNNNNRTG 6 cut(s) 434, 660, 674, 839, 993, 1067
MspI CCGG 1 cut(s) 617
MspR9I CCNGG 3 cut(s) 65, 246, 747
MvaI CCWGG 3 cut(s) 65, 246, 747
MvnI CGCG 2 cut(s) 932, 934
MwoI GCNNNNNNNGC 7 cut(s) 143, 155, 185, 329, 815, 914, 965
NcoI CCATGG 1 cut(s) 1003
NdeI CATATG 1 cut(s) 787
NdeII GATC 3 cut(s) 632, 741, 776
NlaIII CATG 8 cut(s) 298, 337, 569, 603, 665, 992, 1007, 1045
NlaIV GGNNCC 2 cut(s) 77, 894
NmuCI GTSAC 3 cut(s) 304, 698, 830
NspI RCATGY 2 cut(s) 603, 992
OliI CACNNNNGTG 1 cut(s) 1067
PaeI GCATGC 1 cut(s) 992
PasI CCCWGGG 1 cut(s) 746
PceI AGGCCT 1 cut(s) 585
PciI ACATGT 1 cut(s) 599
PcsI WCGNNNNNNNCGW 1 cut(s) 931
PfeI GAWTC 1 cut(s) 354
PkrI GCNGC 4 cut(s) 148, 331, 364, 907
PleI GAGTC 2 cut(s) 261, 481
PpsI GAGTC 2 cut(s) 261, 481
PscI ACATGT 1 cut(s) 599
PshBI ATTAAT 1 cut(s) 972
Psp124BI GAGCTC 1 cut(s) 26
Psp6I CCWGG 3 cut(s) 63, 244, 745
PspGI CCWGG 3 cut(s) 63, 244, 745
PspN4I GGNNCC 2 cut(s) 77, 894
PspPI GGNCC 4 cut(s) 242, 449, 557, 764
PstI CTGCAG 1 cut(s) 181
PsuI RGATCY 2 cut(s) 741, 776
RsaI GTAC 4 cut(s) 235, 540, 715, 1022
RsaNI GTAC 4 cut(s) 234, 539, 714, 1021
RseI CAYNNNNRTG 6 cut(s) 434, 660, 674, 839, 993, 1067
SacI GAGCTC 1 cut(s) 26
SaqAI TTAA 2 cut(s) 738, 972
SatI GCNGC 4 cut(s) 147, 330, 363, 906
Sau3AI GATC 3 cut(s) 632, 741, 776
Sau96I GGNCC 4 cut(s) 242, 449, 557, 764
ScaI AGTACT 1 cut(s) 1022
SchI GAGTC 2 cut(s) 262, 482
ScrFI CCNGG 3 cut(s) 65, 246, 747
SduI GDGCHC 5 cut(s) 26, 78, 119, 174, 1074
SfaNI GCATC 5 cut(s) 175, 274, 831, 904, 936
SfcI CTRYAG 3 cut(s) 177, 543, 1065
SinI GGWCC 2 cut(s) 449, 764
SmiMI CAYNNNNRTG 6 cut(s) 434, 660, 674, 839, 993, 1067
SphI GCATGC 1 cut(s) 992
Sse9I AATT 3 cut(s) 88, 938, 969
SseBI AGGCCT 1 cut(s) 585
SspI AATATT 1 cut(s) 628
SspMI CTAG 1 cut(s) 366
SstI GAGCTC 1 cut(s) 26
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 63, 244, 745
StyI CCWWGG 2 cut(s) 909, 1003
TaaI ACNGT 3 cut(s) 49, 238, 1066
TaiI ACGT 1 cut(s) 1016
TaqI TCGA 3 cut(s) 195, 282, 719
TasI AATT 3 cut(s) 88, 938, 969
TatI WGTACW 1 cut(s) 1020
TfiI GAWTC 1 cut(s) 354
Tru1I TTAA 2 cut(s) 738, 972
Tru9I TTAA 2 cut(s) 738, 972
TscAI CASTG 3 cut(s) 426, 515, 1069
TseFI GTSAC 3 cut(s) 304, 698, 830
TseI GCWGC 4 cut(s) 146, 329, 362, 905
Tsp45I GTSAC 3 cut(s) 304, 698, 830
TspDTI ATGAA 3 cut(s) 456, 774, 813
TspGWI ACGGA 1 cut(s) 843
TspRI CASTG 3 cut(s) 426, 515, 1069
VpaK11BI GGWCC 2 cut(s) 449, 764
VspI ATTAAT 1 cut(s) 972
XagI CCTNNNNNAGG 1 cut(s) 771
XapI RAATTY 2 cut(s) 88, 938
XceI RCATGY 2 cut(s) 603, 992
XspI CTAG 1 cut(s) 366
ZrmI AGTACT 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.