RLG00000026799

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008429
Physical Location & Seq
Forward (+)
6127374 .. 6130265
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026799

Sequence Viewer

Length: 1074 bp
ATGGCTACCAAAACTCTGAGCTCCGCCTGTTTCGTGACCCTTACATTTTTACTTCTCTTCCTGGTTACAGGGCACCATGCTTTGAATTCTGGGCCATACCCTGTGGTGGTGAGCACTTGGCCTTTCGTAGAGGCTGTCAGAGCTGCTTGGAGGGCTGCTGATAATGGGCTCTCTGCAGTTGATGCAGTTGTTGAGGGCTGTTTGGCCTGTGAGGAACTCAGATGTGATGGTACAGTTGGGCCTGGTGGGAGTCCAGATGAGAATGGAGAGACAACAATTGATGCTCTGGTCATGGATGGGGTGACAATGGAGGTTGGAGCTGTTGCTGCCATGAGGTATGTCAAAGATGGAATCAAAGCTGCTAGGTTAGTTATGCAACATACTGAACACACTCTGCTAGTTGGAGAGCAAGCCTCAGTGTTTGCTATTTCAATGGGTCTTCCAGGACCGACAAACCTTAGTTCATCAGAGTCGATGGAGAAGTGGATTAAATGGAAAGAAAATCACTGCCAACCTAACTTTTGGAAAGACGTTGTACCTAAAGGTGGATGTGGCCCTTATCATGCAAAGGACTTTGTAGGCCTTTATGATGAGACATGTTCAAATACCACTCCAGTAGGCAATATTAGATCCAGTTCTCATATTGGTCTTCACAACCATGACACCATATCAATGGCAGTTATTGATAAAATGGGTCACATTGCTGTTGGTACCTCGACTAATGGAGCCACATTCAAGATCCCAGGGAGGGTGGGTGATGGACCTATTGCAGGATCTTCTTCATATGCTGATGATGAAGTGGGTGGTTGTGGTGCAACTGGAGATGGTGACATCATGATGCGTTTCCTTCCATGTTATCAAGTTGTGGAGAGTATGAGATTAGGGATGGAGCCTAAGCTTGCTGCCAAGGATGCAATATCACGCATTGCACGAAAATTCCCTGATTTTGTGGGAGCTGTTTTTGCAATTAATAAGAATGGCGTGCATGCTGGTGCTTGCCATGGGTGGACATTTCAGTACTCGGTGAGAAACCCAGACATGGATGATGTGAAGGTTTACACGGTATTGCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00511 ko04142 map00511 map04142
Pfam Domains
Protein Families
Asparaginase_2

Protein Analysis

358

Amino Acids

38.02

Weight (kDa)

5.61

Isoelectric Point (pI)

25.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 34 - 334 5.9e-74 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 710
AccB1I GGYRCC 2 cut(s) 72, 710
AciI CCGC 1 cut(s) 24
AclWI GGATC 3 cut(s) 624, 733, 781
AcsI RAATTY 2 cut(s) 85, 935
AfaI GTAC 4 cut(s) 232, 537, 712, 1019
AfiI CCNNNNNNNGG 5 cut(s) 106, 545, 748, 770, 1039
AflIII ACRYGT 1 cut(s) 596
AgsI TTSAA 4 cut(s) 85, 432, 603, 736
AjnI CCWGG 4 cut(s) 60, 241, 442, 742
AluBI AGCT 6 cut(s) 21, 143, 320, 359, 898, 956
AluI AGCT 6 cut(s) 21, 143, 320, 359, 898, 956
Alw21I GWGCWC 2 cut(s) 23, 116
Alw26I GTCTC 2 cut(s) 263, 587
AlwI GGATC 3 cut(s) 624, 733, 781
AoxI GGCC 6 cut(s) 92, 119, 204, 239, 553, 580
ApeKI GCWGC 5 cut(s) 143, 155, 326, 359, 902
ApoI RAATTY 2 cut(s) 85, 935
AseI ATTAAT 1 cut(s) 969
Asp718I GGTACC 1 cut(s) 710
AspS9I GGNCC 5 cut(s) 92, 239, 446, 554, 761
AsuHPI GGTGA 5 cut(s) 121, 313, 767, 839, 1036
AvaII GGWCC 2 cut(s) 446, 761
BaeGI GKGCMC 1 cut(s) 75
BanI GGYRCC 2 cut(s) 72, 710
BanII GRGCYC 2 cut(s) 23, 171
BbsI GAAGAC 2 cut(s) 431, 641
Bbv12I GWGCWC 2 cut(s) 23, 116
BbvI GCAGC 5 cut(s) 130, 142, 313, 346, 889
BccI CCATC 7 cut(s) 221, 290, 341, 469, 752, 818, 880
BciT130I CCWGG 4 cut(s) 62, 243, 444, 744
BcoDI GTCTC 2 cut(s) 263, 587
BfaI CTAG 2 cut(s) 363, 398
BfmI CTRYAG 1 cut(s) 174
BisI GCNGC 5 cut(s) 144, 156, 327, 360, 903
BlsI GCNGC 5 cut(s) 145, 157, 328, 361, 904
BmcAI AGTACT 1 cut(s) 1019
Bme1390I CCNGG 4 cut(s) 62, 243, 444, 744
Bme18I GGWCC 2 cut(s) 446, 761
BmgT120I GGNCC 5 cut(s) 92, 239, 446, 554, 761
BmiI GGNNCC 4 cut(s) 74, 712, 727, 891
BmrFI CCNGG 4 cut(s) 62, 243, 444, 744
BmsI GCATC 4 cut(s) 172, 271, 828, 901
BpiI GAAGAC 2 cut(s) 431, 641
BplI GAGNNNNNCTC 2 cut(s) 398, 430
BpmI CTGGAG 2 cut(s) 597, 840
Bpu10I CCTNAGC 1 cut(s) 894
BsaJI CCNNGG 4 cut(s) 742, 743, 906, 1000
BsaXI ACNNNNNCTCC 2 cut(s) 739, 769
Bsc4I CCNNNNNNNGG 5 cut(s) 106, 545, 748, 770, 1039
Bse1I ACTGG 3 cut(s) 614, 633, 823
Bse3DI GCAATG 2 cut(s) 699, 924
BseBI CCWGG 4 cut(s) 62, 243, 444, 744
BseDI CCNNGG 4 cut(s) 742, 743, 906, 1000
BseGI GGATG 5 cut(s) 301, 554, 891, 916, 1048
BseLI CCNNNNNNNGG 5 cut(s) 106, 545, 748, 770, 1039
BseMI GCAATG 2 cut(s) 699, 924
BseMII CTCAG 3 cut(s) 8, 232, 429
BseNI ACTGG 3 cut(s) 614, 633, 823
BseSI GKGCMC 1 cut(s) 75
BseXI GCAGC 5 cut(s) 130, 142, 313, 346, 889
BshFI GGCC 6 cut(s) 94, 121, 206, 241, 555, 582
BshNI GGYRCC 2 cut(s) 72, 710
BsiHKAI GWGCWC 2 cut(s) 23, 116
BslI CCNNNNNNNGG 5 cut(s) 106, 545, 748, 770, 1039
BsmAI GTCTC 2 cut(s) 263, 587
BsnI GGCC 6 cut(s) 94, 121, 206, 241, 555, 582
Bsp1286I GDGCHC 4 cut(s) 23, 75, 116, 171
Bsp143I GATC 3 cut(s) 629, 738, 773
Bsp19I CCATGG 1 cut(s) 1000
BspACI CCGC 1 cut(s) 24
BspANI GGCC 6 cut(s) 94, 121, 206, 241, 555, 582
BspCNI CTCAG 3 cut(s) 9, 231, 428
BspHI TCATGA 1 cut(s) 834
BspLI GGNNCC 4 cut(s) 74, 712, 727, 891
BspMAI CTGCAG 1 cut(s) 178
BspPI GGATC 3 cut(s) 624, 733, 781
BspT107I GGYRCC 2 cut(s) 72, 710
BsrDI GCAATG 2 cut(s) 699, 924
BsrI ACTGG 3 cut(s) 614, 633, 823
BssECI CCNNGG 4 cut(s) 742, 743, 906, 1000
BssMI GATC 3 cut(s) 629, 738, 773
BssT1I CCWWGG 2 cut(s) 906, 1000
Bst2UI CCWGG 4 cut(s) 62, 243, 444, 744
Bst4CI ACNGT 2 cut(s) 235, 1063
Bst6I CTCTTC 1 cut(s) 62
BstAPI GCANNNNNTGC 1 cut(s) 182
BstC8I GCNNGC 5 cut(s) 411, 900, 983, 987, 997
BstDEI CTNAG 5 cut(s) 17, 218, 415, 458, 894
BstDSI CCRYGG 1 cut(s) 1000
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 5 cut(s) 301, 554, 891, 916, 1048
BstKTI GATC 3 cut(s) 632, 741, 776
BstMAI GTCTC 2 cut(s) 263, 587
BstMBI GATC 3 cut(s) 629, 738, 773
BstMWI GCNNNNNNNGC 6 cut(s) 140, 152, 182, 326, 911, 962
BstNI CCWGG 4 cut(s) 62, 243, 444, 744
BstNSI RCATGY 2 cut(s) 600, 989
BstSCI CCNGG 4 cut(s) 60, 241, 442, 742
BstSFI CTRYAG 1 cut(s) 174
BstSLI GKGCMC 1 cut(s) 75
BstV1I GCAGC 5 cut(s) 130, 142, 313, 346, 889
BstV2I GAAGAC 2 cut(s) 431, 641
BstX2I RGATCY 3 cut(s) 629, 738, 773
BstXI CCANNNNNNTGG 1 cut(s) 673
BstYI RGATCY 3 cut(s) 629, 738, 773
BsuRI GGCC 6 cut(s) 94, 121, 206, 241, 555, 582
BtgI CCRYGG 1 cut(s) 1000
BtsCI GGATG 5 cut(s) 301, 554, 891, 916, 1048
BtsI GCAGTG 1 cut(s) 505
BtsIMutI CAGTG 2 cut(s) 423, 505
Cac8I GCNNGC 5 cut(s) 411, 900, 983, 987, 997
CciI TCATGA 1 cut(s) 834
Cfr13I GGNCC 5 cut(s) 92, 239, 446, 554, 761
Csp6I GTAC 4 cut(s) 231, 536, 711, 1018
CviQI GTAC 4 cut(s) 231, 536, 711, 1018
DdeI CTNAG 5 cut(s) 17, 218, 415, 458, 894
DpnI GATC 3 cut(s) 631, 740, 775
DpnII GATC 3 cut(s) 629, 738, 773
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
EciI GGCGGA 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 2 cut(s) 906, 1000
Eco147I AGGCCT 1 cut(s) 582
Eco24I GRGCYC 2 cut(s) 23, 171
Eco47I GGWCC 2 cut(s) 446, 761
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 4 cut(s) 60, 241, 442, 742
EcoT14I CCWWGG 2 cut(s) 906, 1000
EcoT38I GRGCYC 2 cut(s) 23, 171
ErhI CCWWGG 2 cut(s) 906, 1000
FauNDI CATATG 1 cut(s) 784
Fnu4HI GCNGC 5 cut(s) 144, 156, 327, 360, 903
FokI GGATG 5 cut(s) 308, 561, 898, 923, 1055
FriOI GRGCYC 2 cut(s) 23, 171
Fsp4HI GCNGC 5 cut(s) 144, 156, 327, 360, 903
FspBI CTAG 2 cut(s) 363, 398
GluI GCNGC 5 cut(s) 144, 156, 327, 360, 903
GsuI CTGGAG 2 cut(s) 597, 840
HaeIII GGCC 6 cut(s) 94, 121, 206, 241, 555, 582
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 3 cut(s) 250, 351, 470
HphI GGTGA 5 cut(s) 121, 313, 767, 839, 1036
Hpy166II GTNNAC 2 cut(s) 1008, 1057
Hpy188I TCNGA 4 cut(s) 18, 140, 221, 469
Hpy188III TCNNGA 4 cut(s) 34, 254, 736, 835
Hpy8I GTNNAC 2 cut(s) 1008, 1057
HpyAV CCTTC 2 cut(s) 857, 1045
HpyCH4III ACNGT 2 cut(s) 235, 1063
HpyCH4IV ACGT 1 cut(s) 531
HpyF10VI GCNNNNNNNGC 6 cut(s) 140, 152, 182, 326, 911, 962
HpyF3I CTNAG 5 cut(s) 17, 218, 415, 458, 894
HpySE526I ACGT 1 cut(s) 531
KpnI GGTACC 1 cut(s) 714
Kzo9I GATC 3 cut(s) 629, 738, 773
LmnI GCTCC 5 cut(s) 26, 317, 725, 889, 953
Lsp1109I GCAGC 5 cut(s) 130, 142, 313, 346, 889
LweI GCATC 4 cut(s) 172, 271, 828, 901
MaeI CTAG 2 cut(s) 363, 398
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 5 cut(s) 34, 64, 301, 695, 827
MalI GATC 3 cut(s) 631, 740, 775
MboI GATC 3 cut(s) 629, 738, 773
MboII GAAGA 5 cut(s) 49, 431, 641, 768, 771
MfeI CAATTG 1 cut(s) 276
MflI RGATCY 3 cut(s) 629, 738, 773
MhlI GDGCHC 4 cut(s) 23, 75, 116, 171
MluCI AATT 4 cut(s) 85, 276, 935, 966
MlyI GAGTC 2 cut(s) 259, 479
MmeI TCCRAC 2 cut(s) 295, 382
MnlI CCTC 9 cut(s) 124, 144, 187, 205, 304, 327, 424, 724, 741
MseI TTAA 2 cut(s) 489, 969
MslI CAYNNNNRTG 4 cut(s) 657, 671, 836, 990
MspR9I CCNGG 4 cut(s) 62, 243, 444, 744
MunI CAATTG 1 cut(s) 276
MvaI CCWGG 4 cut(s) 62, 243, 444, 744
MwoI GCNNNNNNNGC 6 cut(s) 140, 152, 182, 326, 911, 962
NcoI CCATGG 1 cut(s) 1000
NdeI CATATG 1 cut(s) 784
NdeII GATC 3 cut(s) 629, 738, 773
NlaIV GGNNCC 4 cut(s) 74, 712, 727, 891
NmuCI GTSAC 4 cut(s) 34, 301, 695, 827
NspI RCATGY 2 cut(s) 600, 989
PaeI GCATGC 1 cut(s) 989
PagI TCATGA 1 cut(s) 834
PasI CCCWGGG 1 cut(s) 743
PceI AGGCCT 1 cut(s) 582
PciI ACATGT 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 928
PfeI GAWTC 1 cut(s) 351
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 5 cut(s) 145, 157, 328, 361, 904
PleI GAGTC 2 cut(s) 258, 478
PpsI GAGTC 2 cut(s) 258, 478
PscI ACATGT 1 cut(s) 596
PshBI ATTAAT 1 cut(s) 969
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 4 cut(s) 60, 241, 442, 742
PspGI CCWGG 4 cut(s) 60, 241, 442, 742
PspN4I GGNNCC 4 cut(s) 74, 712, 727, 891
PspPI GGNCC 5 cut(s) 92, 239, 446, 554, 761
PstI CTGCAG 1 cut(s) 178
PsuI RGATCY 3 cut(s) 629, 738, 773
RsaI GTAC 4 cut(s) 232, 537, 712, 1019
RsaNI GTAC 4 cut(s) 231, 536, 711, 1018
RseI CAYNNNNRTG 4 cut(s) 657, 671, 836, 990
SacI GAGCTC 1 cut(s) 23
SaqAI TTAA 2 cut(s) 489, 969
SatI GCNGC 5 cut(s) 144, 156, 327, 360, 903
Sau3AI GATC 3 cut(s) 629, 738, 773
Sau96I GGNCC 5 cut(s) 92, 239, 446, 554, 761
ScaI AGTACT 1 cut(s) 1019
SchI GAGTC 2 cut(s) 259, 479
ScrFI CCNGG 4 cut(s) 62, 243, 444, 744
SduI GDGCHC 4 cut(s) 23, 75, 116, 171
SfaNI GCATC 4 cut(s) 172, 271, 828, 901
SfcI CTRYAG 1 cut(s) 174
SinI GGWCC 2 cut(s) 446, 761
SmiMI CAYNNNNRTG 4 cut(s) 657, 671, 836, 990
SphI GCATGC 1 cut(s) 989
Sse9I AATT 4 cut(s) 85, 276, 935, 966
SseBI AGGCCT 1 cut(s) 582
SsiI CCGC 1 cut(s) 24
SspI AATATT 1 cut(s) 625
SspMI CTAG 2 cut(s) 363, 398
SstI GAGCTC 1 cut(s) 23
StuI AGGCCT 1 cut(s) 582
StyD4I CCNGG 4 cut(s) 60, 241, 442, 742
StyI CCWWGG 2 cut(s) 906, 1000
TaaI ACNGT 2 cut(s) 235, 1063
TaiI ACGT 1 cut(s) 534
TaqI TCGA 2 cut(s) 473, 716
TaqII GACCGA 1 cut(s) 463
TasI AATT 4 cut(s) 85, 276, 935, 966
TatI WGTACW 1 cut(s) 1017
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 2 cut(s) 489, 969
Tru9I TTAA 2 cut(s) 489, 969
TscAI CASTG 2 cut(s) 423, 512
TseFI GTSAC 4 cut(s) 34, 301, 695, 827
TseI GCWGC 5 cut(s) 143, 155, 326, 359, 902
Tsp45I GTSAC 4 cut(s) 34, 301, 695, 827
TspDTI ATGAA 3 cut(s) 453, 771, 810
TspRI CASTG 2 cut(s) 423, 512
VpaK11BI GGWCC 2 cut(s) 446, 761
VspI ATTAAT 1 cut(s) 969
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 2 cut(s) 85, 935
XceI RCATGY 2 cut(s) 600, 989
XspI CTAG 2 cut(s) 363, 398
ZrmI AGTACT 1 cut(s) 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.