AT5G64020

function) domain. TBL gene family has 46 members, two of which (TBR AT5G06700 and TBL3 AT5G01360) have been shown to be involved in the synthesis and deposition of secondary wall cellulose, presumably by influencing the esterification state of pectic polymers. A nomenclature for this gene family has been proposed (Volker Bischoff Wolf Scheible, 2010, personal communication)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008475
Physical Location & Seq
Reverse (-)
25620246 .. 25622646
2401 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64020.1

Sequence Viewer

Length: 1227 bp
ATGTTTGGTGGAAAAAGTCATATACTCAGAGGGAGTGTATCACTGGCCTTGATCGTCCTTATTCTTTTGGTCATTATACTCCTGGTTTCTGAGGAAAACCCGCTTCGTGATTCTCTCTTTGAAGTAAAACGTCAGTTTTCATCTTCTTCTTCTTCTTCTTCTTCAGTTTGTAACTTTGCAAAGGGAAAATGGGTTGAAGACCGGAAGAGACCATTGTATTCTGGTTTTGAATGTAAACAATGGTTATCATCCATGTGGTCATGTAGAATAATGGGCAGACCCGATTTCTCATTTGAGGGTTACCGTTGGCAGCCAGAAGGTTGCAACATGCCACAGTTTGACAGATTCACTTTCTTGACAAGAATGCAGAACAAGACGATAGCATTTATAGGAGACTCATTGGGGCGGCAACAGTTTCAATCCCTAATGTGTATGGCCAGTGGTGGTGAAGATAGCCCAGAGGTTCAAAACGTGGGATGGGAGTACGGTTTGGTGAAAGCCAAAGGAGCTCTCCGTCCTGATGGTTGGGCTTATCGGTTCCCAACCACAAACACGACGATCTTGTATTATTGGTCAGCTAGCTTATCAGATTTGGTACCCATGAACAATACAGATCCACCTAGTCTTACTGCAATGCATCTAGATCGTCCACCAGCATTCATGAGAAACTACCTTCACCGTTTCGATGTATTGGTTCTAAACACTGGTCACCATTGGAACAGAGGTAAGATTGAAGGAAACCATTGGGTGATGCATGTAAATGGAACACAGGTCGAAGGCGAATATCTCAAAGATATCAGAAACGCCAAGGATTTTACAATACACAGCGTTGCGAAGTGGCTCGATGCACAGCTTCCATTGCATCCGCGGTTGAAAGCTTTCTTTAGGACAATTTCTCCGAGGCATTTTAAAAACGGAGATTGGAATACAGGTGGAAACTGTAACAACACGGTTCCTTTGTCTAGAGGCAGCGAAATCACAGGGGATGATGGATCGATCGATGCAACAGTTGAGAGTGCTGTGAACGGGACAAGGATCAAGATTCTTGACATAACTGCACTTTCTGAGCTAAGAGACGAAGCTCATATCTCAGGGTCTAAACTCAAACCCCGAAAACCGAAGAAGGCAAGTAACGTGACCTCAACTCCAACGATCAACGATTGCTTGCATTGGTGCTTACCAGGGATCCCAGATACTTGGAATGAACTTTTCATTGCTCAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

408

Amino Acids

46.1

Weight (kDa)

8.82

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 56 - 109 1.2e-16 PMR5 N terminal Domain
PC-Esterase PF13839 110 - 406 1.7e-73 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 595
AccB1I GGYRCC 1 cut(s) 595
AccII CGCG 1 cut(s) 868
AciI CCGC 4 cut(s) 101, 406, 866, 868
AclWI GGATC 5 cut(s) 608, 1000, 1043, 1180, 1193
AcoI YGGCCR 1 cut(s) 435
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 2 cut(s) 485, 597
AgsI TTSAA 7 cut(s) 122, 197, 230, 419, 467, 734, 874
AjnI CCWGG 2 cut(s) 81, 1180
AluBI AGCT 7 cut(s) 509, 578, 582, 853, 878, 1069, 1082
AluI AGCT 7 cut(s) 509, 578, 582, 853, 878, 1069, 1082
Alw21I GWGCWC 1 cut(s) 511
Alw26I GTCTC 3 cut(s) 202, 387, 1068
AlwI GGATC 5 cut(s) 608, 1000, 1043, 1180, 1193
AoxI GGCC 2 cut(s) 45, 435
ApeKI GCWGC 2 cut(s) 310, 969
Asp700I GAANNNNTTC 1 cut(s) 878
Asp718I GGTACC 1 cut(s) 595
AsuHPI GGTGA 5 cut(s) 458, 505, 668, 701, 760
AsuNHI GCTAGC 1 cut(s) 578
BalI TGGCCA 1 cut(s) 437
BamHI GGATCC 1 cut(s) 1185
BanI GGYRCC 1 cut(s) 595
BanII GRGCYC 1 cut(s) 511
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 322, 981
BccI CCATC 3 cut(s) 471, 515, 983
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 2 cut(s) 83, 1182
BcoDI GTCTC 3 cut(s) 202, 387, 1068
BfaI CTAG 4 cut(s) 579, 621, 641, 963
BisI GCNGC 3 cut(s) 311, 407, 970
BlsI GCNGC 3 cut(s) 312, 408, 971
Bme1390I CCNGG 2 cut(s) 83, 1182
BmiI GGNNCC 4 cut(s) 539, 597, 954, 1187
BmrFI CCNGG 2 cut(s) 83, 1182
BmsI GCATC 5 cut(s) 646, 741, 835, 871, 991
BmtI GCTAGC 1 cut(s) 582
BpiI GAAGAC 1 cut(s) 204
Bsa29I ATCGAT 2 cut(s) 995, 999
BsaI GGTCTC 1 cut(s) 202
BsaJI CCNNGG 4 cut(s) 807, 866, 899, 1181
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 4 cut(s) 880, 910, 1129, 1159
Bse1I ACTGG 3 cut(s) 48, 438, 709
Bse3DI GCAATG 3 cut(s) 639, 857, 1212
BseBI CCWGG 2 cut(s) 83, 1182
BseCI ATCGAT 2 cut(s) 995, 999
BseDI CCNNGG 4 cut(s) 807, 866, 899, 1181
BseGI GGATG 4 cut(s) 248, 482, 862, 991
BseMI GCAATG 3 cut(s) 639, 857, 1212
BseMII CTCAG 4 cut(s) 40, 81, 1056, 1104
BseNI ACTGG 3 cut(s) 48, 438, 709
BseXI GCAGC 2 cut(s) 322, 981
BsgI GTGCAG 1 cut(s) 1041
Bsh1236I CGCG 1 cut(s) 868
Bsh1285I CGRYCG 1 cut(s) 999
BshFI GGCC 2 cut(s) 47, 437
BshNI GGYRCC 1 cut(s) 595
BshVI ATCGAT 2 cut(s) 995, 999
BsiEI CGRYCG 1 cut(s) 999
BsiHKAI GWGCWC 1 cut(s) 511
BsiSI CCGG 1 cut(s) 202
BslFI GGGAC 1 cut(s) 1042
BsmAI GTCTC 3 cut(s) 202, 387, 1068
BsmBI CGTCTC 1 cut(s) 1068
BsmFI GGGAC 1 cut(s) 1042
BsmI GAATGC 2 cut(s) 369, 656
BsnI GGCC 2 cut(s) 47, 437
Bso31I GGTCTC 1 cut(s) 202
Bsp1286I GDGCHC 1 cut(s) 511
Bsp143I GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
BspACI CCGC 4 cut(s) 101, 406, 866, 868
BspANI GGCC 2 cut(s) 47, 437
BspCNI CTCAG 4 cut(s) 39, 82, 1057, 1103
BspDI ATCGAT 2 cut(s) 995, 999
BspFNI CGCG 1 cut(s) 868
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 4 cut(s) 539, 597, 954, 1187
BspOI GCTAGC 1 cut(s) 582
BspPI GGATC 5 cut(s) 608, 1000, 1043, 1180, 1193
BspT107I GGYRCC 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 202
BsrDI GCAATG 3 cut(s) 639, 857, 1212
BsrI ACTGG 3 cut(s) 48, 438, 709
BssECI CCNNGG 4 cut(s) 807, 866, 899, 1181
BssMI GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
BssT1I CCWWGG 1 cut(s) 807
Bst2UI CCWGG 2 cut(s) 83, 1182
Bst4CI ACNGT 8 cut(s) 305, 336, 414, 488, 680, 941, 952, 1009
Bst6I CTCTTC 1 cut(s) 200
BstC8I GCNNGC 2 cut(s) 580, 1166
BstDEI CTNAG 6 cut(s) 26, 90, 1065, 1070, 1090, 1218
BstDSI CCRYGG 1 cut(s) 866
BstEII GGTNACC 2 cut(s) 299, 707
BstF5I GGATG 4 cut(s) 248, 482, 862, 991
BstFNI CGCG 1 cut(s) 868
BstKTI GATC 9 cut(s) 54, 561, 616, 646, 995, 999, 1038, 1155, 1188
BstMAI GTCTC 3 cut(s) 202, 387, 1068
BstMBI GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
BstMCI CGRYCG 1 cut(s) 999
BstMWI GCNNNNNNNGC 2 cut(s) 506, 859
BstNI CCWGG 2 cut(s) 83, 1182
BstNSI RCATGY 2 cut(s) 331, 758
BstPI GGTNACC 2 cut(s) 299, 707
BstSCI CCNGG 2 cut(s) 81, 1180
BstUI CGCG 1 cut(s) 868
BstV1I GCAGC 2 cut(s) 322, 981
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 2 cut(s) 613, 1185
BstXI CCANNNNNNTGG 1 cut(s) 1197
BstYI RGATCY 2 cut(s) 613, 1185
Bsu15I ATCGAT 2 cut(s) 995, 999
BsuRI GGCC 2 cut(s) 47, 437
BsuTUI ATCGAT 2 cut(s) 995, 999
BtgI CCRYGG 1 cut(s) 866
BtsCI GGATG 4 cut(s) 248, 482, 862, 991
BtsIMutI CAGTG 3 cut(s) 41, 445, 702
Cac8I GCNNGC 2 cut(s) 580, 1166
CciI TCATGA 1 cut(s) 660
Cfr42I CCGCGG 1 cut(s) 869
ClaI ATCGAT 2 cut(s) 995, 999
Csp6I GTAC 2 cut(s) 484, 596
CviAII CATG 6 cut(s) 253, 261, 328, 601, 661, 755
CviQI GTAC 2 cut(s) 484, 596
DdeI CTNAG 6 cut(s) 26, 90, 1065, 1070, 1090, 1218
DpnI GATC 9 cut(s) 53, 560, 615, 645, 994, 998, 1037, 1154, 1187
DpnII GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
DraI TTTAAA 1 cut(s) 910
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 509
Eco130I CCWWGG 1 cut(s) 807
Eco24I GRGCYC 1 cut(s) 511
Eco31I GGTCTC 1 cut(s) 202
Eco32I GATATC 1 cut(s) 796
Eco53kI GAGCTC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 147
Eco91I GGTNACC 2 cut(s) 299, 707
EcoICRI GAGCTC 1 cut(s) 509
EcoO65I GGTNACC 2 cut(s) 299, 707
EcoRII CCWGG 2 cut(s) 81, 1180
EcoRV GATATC 1 cut(s) 796
EcoT14I CCWWGG 1 cut(s) 807
EcoT22I ATGCAT 2 cut(s) 639, 756
EcoT38I GRGCYC 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 807
Esp3I CGTCTC 1 cut(s) 1068
FaeI CATG 6 cut(s) 256, 264, 331, 604, 664, 758
FaqI GGGAC 1 cut(s) 1042
FatI CATG 6 cut(s) 252, 260, 327, 600, 660, 754
FauI CCCGC 1 cut(s) 108
Fnu4HI GCNGC 3 cut(s) 311, 407, 970
FokI GGATG 4 cut(s) 235, 489, 849, 998
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 3 cut(s) 311, 407, 970
FspBI CTAG 4 cut(s) 579, 621, 641, 963
GluI GCNGC 3 cut(s) 311, 407, 970
HaeIII GGCC 2 cut(s) 47, 437
HapII CCGG 1 cut(s) 202
Hin1II CATG 6 cut(s) 256, 264, 331, 604, 664, 758
HindIII AAGCTT 1 cut(s) 876
HinfI GANTC 4 cut(s) 110, 345, 395, 1042
HpaII CCGG 1 cut(s) 202
HphI GGTGA 5 cut(s) 458, 505, 668, 701, 760
Hpy166II GTNNAC 3 cut(s) 236, 650, 1024
Hpy188I TCNGA 7 cut(s) 29, 91, 589, 800, 900, 1066, 1221
Hpy188III TCNNGA 8 cut(s) 107, 355, 518, 641, 661, 963, 1039, 1046
Hpy8I GTNNAC 3 cut(s) 236, 650, 1024
Hpy99I CGWCG 1 cut(s) 559
HpyAV CCTTC 5 cut(s) 311, 683, 728, 770, 1117
HpyCH4III ACNGT 8 cut(s) 305, 336, 414, 488, 680, 941, 952, 1009
HpyCH4IV ACGT 3 cut(s) 130, 471, 1134
HpyF10VI GCNNNNNNNGC 2 cut(s) 506, 859
HpyF3I CTNAG 6 cut(s) 26, 90, 1065, 1070, 1090, 1218
HpySE526I ACGT 3 cut(s) 130, 471, 1134
Hsp92II CATG 6 cut(s) 256, 264, 331, 604, 664, 758
KpnI GGTACC 1 cut(s) 599
KspI CCGCGG 1 cut(s) 869
Kzo9I GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
LmnI GCTCC 1 cut(s) 506
Lsp1109I GCAGC 2 cut(s) 322, 981
LweI GCATC 5 cut(s) 646, 741, 835, 871, 991
MaeI CTAG 4 cut(s) 579, 621, 641, 963
MaeII ACGT 3 cut(s) 130, 471, 1134
MaeIII GTNAC 6 cut(s) 170, 299, 707, 941, 1130, 1135
MalI GATC 9 cut(s) 53, 560, 615, 645, 994, 998, 1037, 1154, 1187
MboI GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
MflI RGATCY 2 cut(s) 613, 1185
MhlI GDGCHC 1 cut(s) 511
MlsI TGGCCA 1 cut(s) 437
MluCI AATT 1 cut(s) 891
MluNI TGGCCA 1 cut(s) 437
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 1 cut(s) 1172
MnlI CCTC 8 cut(s) 23, 85, 289, 454, 716, 894, 959, 1150
Mox20I TGGCCA 1 cut(s) 437
Mph1103I ATGCAT 2 cut(s) 639, 756
MroXI GAANNNNTTC 1 cut(s) 878
MscI TGGCCA 1 cut(s) 437
MseI TTAA 1 cut(s) 909
MslI CAYNNNNRTG 2 cut(s) 253, 759
Msp20I TGGCCA 1 cut(s) 437
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 1 cut(s) 202
MspR9I CCNGG 2 cut(s) 83, 1182
Mva1269I GAATGC 2 cut(s) 369, 656
MvaI CCWGG 2 cut(s) 83, 1182
MvnI CGCG 1 cut(s) 868
MwoI GCNNNNNNNGC 2 cut(s) 506, 859
NdeII GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
NheI GCTAGC 1 cut(s) 578
NlaIII CATG 6 cut(s) 256, 264, 331, 604, 664, 758
NlaIV GGNNCC 4 cut(s) 539, 597, 954, 1187
NmuCI GTSAC 2 cut(s) 707, 1135
NsiI ATGCAT 2 cut(s) 639, 756
NspI RCATGY 2 cut(s) 331, 758
PagI TCATGA 1 cut(s) 660
PctI GAATGC 2 cut(s) 369, 656
PdmI GAANNNNTTC 1 cut(s) 878
PfeI GAWTC 3 cut(s) 110, 345, 1042
PkrI GCNGC 3 cut(s) 312, 408, 971
Ple19I CGATCG 1 cut(s) 999
PleI GAGTC 1 cut(s) 389
PpsI GAGTC 1 cut(s) 389
Psp124BI GAGCTC 1 cut(s) 511
Psp6I CCWGG 2 cut(s) 81, 1180
PspEI GGTNACC 2 cut(s) 299, 707
PspGI CCWGG 2 cut(s) 81, 1180
PspN4I GGNNCC 4 cut(s) 539, 597, 954, 1187
PsuI RGATCY 2 cut(s) 613, 1185
PvuI CGATCG 1 cut(s) 999
RsaI GTAC 2 cut(s) 485, 597
RsaNI GTAC 2 cut(s) 484, 596
RseI CAYNNNNRTG 2 cut(s) 253, 759
SacI GAGCTC 1 cut(s) 511
SacII CCGCGG 1 cut(s) 869
SaqAI TTAA 1 cut(s) 909
SatI GCNGC 3 cut(s) 311, 407, 970
Sau3AI GATC 9 cut(s) 51, 558, 613, 643, 992, 996, 1035, 1152, 1185
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 2 cut(s) 83, 1182
SduI GDGCHC 1 cut(s) 511
SfaNI GCATC 5 cut(s) 646, 741, 835, 871, 991
Sfr303I CCGCGG 1 cut(s) 869
SgrBI CCGCGG 1 cut(s) 869
SmiMI CAYNNNNRTG 2 cut(s) 253, 759
Sse9I AATT 1 cut(s) 891
SsiI CCGC 4 cut(s) 101, 406, 866, 868
SspMI CTAG 4 cut(s) 579, 621, 641, 963
SstI GAGCTC 1 cut(s) 511
StyD4I CCNGG 2 cut(s) 81, 1180
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 8 cut(s) 305, 336, 414, 488, 680, 941, 952, 1009
TaiI ACGT 3 cut(s) 133, 474, 1137
TaqI TCGA 5 cut(s) 684, 774, 843, 995, 999
TasI AATT 1 cut(s) 891
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 3 cut(s) 110, 345, 1042
Tru1I TTAA 1 cut(s) 909
Tru9I TTAA 1 cut(s) 909
TscAI CASTG 3 cut(s) 48, 445, 709
TseFI GTSAC 2 cut(s) 707, 1135
TseI GCWGC 2 cut(s) 310, 969
Tsp45I GTSAC 2 cut(s) 707, 1135
TspDTI ATGAA 5 cut(s) 129, 617, 649, 1201, 1218
TspGWI ACGGA 2 cut(s) 503, 930
TspRI CASTG 3 cut(s) 48, 445, 709
XbaI TCTAGA 2 cut(s) 640, 962
XceI RCATGY 2 cut(s) 331, 758
XmnI GAANNNNTTC 1 cut(s) 878
XspI CTAG 4 cut(s) 579, 621, 641, 963
Zsp2I ATGCAT 2 cut(s) 639, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.