Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0008475

Physical Location & Seq

Genomic Coordinates Reverse (-)

9756919 .. 9763433

Gene Structure 6515 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1134400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGAAAGGGGGATATAACTACAAATTGATTGGGAAACCAATCTCTTTCACTCTGGCTGCCCTTATATTCACAACAATATTACTTTGGGCGTGGGAGAAAAATCCTTTTGCTGATACTCTACTATTGCCGCGCCAGCGGTTTCCAATGCCTTCCATAGATTTTTTTGGGGACTTCTCAAATAATTCCTCCAAACCTATGAAGACAAATGGAAATATTGAAGGGAAGGATTTACATTTGAATACGGCAGAAGATACAAAAGTAATAGAAAAGTTTGGCACAGCCCCAATAGATTCTATGTATGCATTCTCTCCTGGAATGCATGAAAGTAATGGGAATGCGACATCTTTCCAAACTAAAGTGTGTAATTATGCCAAGGGTAGATGGGTTGCGGACAACAGGCGCCCTTTATATTCTGGGTTTAAATGTAAGCAGTGGTTATCAGAAATGTGGGCGTGTAGACTGACACAACGAACAGATTTTTCTTTTGAGGGTTACCGATGGAAGCCAGCAAATTGTGAAATACCAGATTTTGAACGCTCTGCATTCTTGAGAAGGATGCAGGACAAAACGATTGCTTTCATAGGAGATTCATTGGGTAGACAGCAGTTCCAGTCTTTGATGTGTATGGCAACTGGCGGGGAAGAAAGCCCCGAAGTTGAGAATGTCGGAAAGGAATATGGCCTTGTCAAACGTCGTGGAGCCATTCGTCCTGATGGCTGGGCCTATCGATTCTCTAACACCAATACCACTATCTTATATTATTGGTCAGCAAGCCTGTGTGATCTAGTGCCCCTTAACATCTCAGACCGAGCCAGTGATATTGCCATGCATTTGGATCGTCCCCCGGCTTTCTTGAGACAATACCTTCATCGGTTTGATGTGCTGGTTCTTAATACAGGGCATCATTGGAATAGGGGAAAGATTAATGGTAACCGGTGGGTAATGCATGTAAACGGAAAGCCCAATCAAGAAAAGATGCGTGCAGAAATTGGAAATGCTAAAAATTTTACTGTGTACAGTATTACCAGGTGGGTTGATTCACAACTACCATCACATCCCCGCCTAAAAGCTTTCTTTCGGACAATTTCACCGAGGCATTTCTTCAATGGTGACTGGAATACTGGGGGTACCTGCGACAATACTACTCCGTTGGTCGGAGGAAGTGAAGTAGTCCAGGAAGGATCCAGTGATTCAGTGATCGAGGGTGCTTTGAAAGGTACAAAAGTAAAGTTACTGGATATAACTGCCATTTCTCAATTGAGAGATGAAGGTCACATATCCCACTATACCATTAGAGGAAAAGGAAATGCAGGCACAAGTGATTGCTTACACTGGTGCTTACCTGGAATTCCAGATGCATGGAATGAACTCCTTGTTGCACAGATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

463

Amino Acids

52.32

Weight (kDa)

9.0

Isoelectric Point (pI)

43.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	120 - 173	4.1e-15	PMR5 N terminal Domain
PC-Esterase	PF13839	174 - 460	1.3e-73	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64020
fragaria_vesca	FvH4_1g24540 FvH4_1g24540 FvH4_1g24540 FvH4_1g24540 FvH4_1g24540 FvH4_1g24540
malus_domestica	MD01G1006300.v1.1 MD15G1134400.v1.1
prunus_persica	Prupe.4G274000_v2.0.a1 Prupe.4G274000_v2.0.a1
pyrus_communis	pycom15g12100
rosa_chinensis	RchiOBHm_Chr2g0121951
rosa_laevigata	RLG00000018636
rosa_multiflora	Rmu_sc0003880.1_g000021
rosa_roxburghii	Rroxscaffold_2G00122190
rosa_rugosa	Rorug02G0235000
rosa_samantha	Rh2AG292100 Rh2BG302300 Rh2CG281100 Rh2DG316300
rosa_wichuraiana	Rw2G023420 Rw2G023440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1139
Acc65I	GGTACC	1 cut(s)	1127
AccB1I	GGYRCC	2 cut(s)	399, 1127
AccI	GTMKAC	2 cut(s)	457, 598
AccII	CGCG	1 cut(s)	130
AciI	CCGC	5 cut(s)	128, 136, 389, 636, 1060
AclWI	GGATC	3 cut(s)	843, 1176, 1189
AcsI	RAATTY	2 cut(s)	1003, 1347
AcyI	GRCGYC	1 cut(s)	400
AfaI	GTAC	3 cut(s)	1016, 1129, 1219
AfiI	CCNNNNNNNGG	1 cut(s)	1154
AgeI	ACCGGT	1 cut(s)	933
AgsI	TTSAA	5 cut(s)	218, 238, 533, 1105, 1213
AjnI	CCWGG	4 cut(s)	310, 1025, 1173, 1342
AluBI	AGCT	1 cut(s)	1070
AluI	AGCT	1 cut(s)	1070
Alw26I	GTCTC	1 cut(s)	850
AlwI	GGATC	3 cut(s)	843, 1176, 1189
AoxI	GGCC	2 cut(s)	679, 720
ApeKI	GCWGC	1 cut(s)	56
ApoI	RAATTY	2 cut(s)	1003, 1347
AseI	ATTAAT	1 cut(s)	924
AsiGI	ACCGGT	1 cut(s)	933
Asp718I	GGTACC	1 cut(s)	1127
AspLEI	GCGC	2 cut(s)	132, 402
AspS9I	GGNCC	1 cut(s)	720
AsuC2I	CCSGG	1 cut(s)	845
AsuHPI	GGTGA	2 cut(s)	1080, 1121
BaeGI	GKGCMC	1 cut(s)	792
BaeI	ACNNNNGTAYC	4 cut(s)	243, 276, 1209, 1242
BamHI	GGATCC	1 cut(s)	1181
BanI	GGYRCC	2 cut(s)	399, 1127
BbsI	GAAGAC	1 cut(s)	206
BbvI	GCAGC	1 cut(s)	43
BccI	CCATC	4 cut(s)	375, 492, 707, 1057
BceAI	ACGGC	1 cut(s)	258
BcgI	CGANNNNNNTGC	2 cut(s)	818, 852
BciT130I	CCWGG	4 cut(s)	312, 1027, 1175, 1344
BcnI	CCSGG	1 cut(s)	845
BcoDI	GTCTC	1 cut(s)	850
BfaI	CTAG	1 cut(s)	785
BfoI	RGCGCY	1 cut(s)	403
BfuAI	ACCTGC	1 cut(s)	1139
BisI	GCNGC	2 cut(s)	57, 128
BlsI	GCNGC	2 cut(s)	58, 129
Bme1390I	CCNGG	5 cut(s)	312, 845, 1027, 1175, 1344
BmgT120I	GGNCC	1 cut(s)	720
BmiI	GGNNCC	4 cut(s)	401, 700, 1129, 1183
BmrFI	CCNGG	5 cut(s)	312, 845, 1027, 1175, 1344
BmrI	ACTGGG	1 cut(s)	1131
BmsI	GCATC	4 cut(s)	546, 910, 966, 1345
BmuI	ACTGGG	1 cut(s)	1131
BpiI	GAAGAC	1 cut(s)	206
BpuEI	CTTGAG	2 cut(s)	568, 874
BpuMI	CCSGG	1 cut(s)	845
Bsa29I	ATCGAT	1 cut(s)	727
BsaHI	GRCGYC	1 cut(s)	400
BsaJI	CCNNGG	3 cut(s)	372, 843, 1091
BsaWI	WCCGGW	1 cut(s)	933
Bsc4I	CCNNNNNNNGG	1 cut(s)	1154
Bse118I	RCCGGY	1 cut(s)	933
Bse1I	ACTGG	8 cut(s)	610, 637, 813, 1118, 1126, 1185, 1239, 1337
BseBI	CCWGG	4 cut(s)	312, 1027, 1175, 1344
BseCI	ATCGAT	1 cut(s)	727
BseDI	CCNNGG	3 cut(s)	372, 843, 1091
BseGI	GGATG	2 cut(s)	561, 1054
BseLI	CCNNNNNNNGG	1 cut(s)	1154
BseMII	CTCAG	1 cut(s)	816
BseNI	ACTGG	8 cut(s)	610, 637, 813, 1118, 1126, 1185, 1239, 1337
BseSI	GKGCMC	1 cut(s)	792
BseXI	GCAGC	1 cut(s)	43
BseYI	CCCAGC	1 cut(s)	717
BsgI	GTGCAG	1 cut(s)	1002
Bsh1236I	CGCG	1 cut(s)	130
BshFI	GGCC	2 cut(s)	681, 722
BshNI	GGYRCC	2 cut(s)	399, 1127
BshTI	ACCGGT	1 cut(s)	933
BshVI	ATCGAT	1 cut(s)	727
BsiSI	CCGG	2 cut(s)	845, 934
BslFI	GGGAC	2 cut(s)	182, 825
BslI	CCNNNNNNNGG	1 cut(s)	1154
BsmAI	GTCTC	1 cut(s)	850
BsmFI	GGGAC	2 cut(s)	182, 825
BsmI	GAATGC	4 cut(s)	302, 321, 340, 542
BsnI	GGCC	2 cut(s)	681, 722
Bsp1286I	GDGCHC	1 cut(s)	792
Bsp1407I	TGTACA	1 cut(s)	1014
Bsp143I	GATC	4 cut(s)	781, 835, 1181, 1197
BspACI	CCGC	5 cut(s)	128, 136, 389, 636, 1060
BspANI	GGCC	2 cut(s)	681, 722
BspCNI	CTCAG	1 cut(s)	815
BspDI	ATCGAT	1 cut(s)	727
BspFNI	CGCG	1 cut(s)	130
BspLI	GGNNCC	4 cut(s)	401, 700, 1129, 1183
BspMI	ACCTGC	1 cut(s)	1139
BspPI	GGATC	3 cut(s)	843, 1176, 1189
BspT107I	GGYRCC	2 cut(s)	399, 1127
BsrFI	RCCGGY	1 cut(s)	933
BsrGI	TGTACA	1 cut(s)	1014
BsrI	ACTGG	8 cut(s)	610, 637, 813, 1118, 1126, 1185, 1239, 1337
BssAI	RCCGGY	1 cut(s)	933
BssECI	CCNNGG	3 cut(s)	372, 843, 1091
BssMI	GATC	4 cut(s)	781, 835, 1181, 1197
BssNI	GRCGYC	1 cut(s)	400
BssT1I	CCWWGG	1 cut(s)	372
Bst2UI	CCWGG	4 cut(s)	312, 1027, 1175, 1344
Bst4CI	ACNGT	2 cut(s)	1012, 1019
BstACI	GRCGYC	1 cut(s)	400
BstAUI	TGTACA	1 cut(s)	1014
BstC8I	GCNNGC	5 cut(s)	134, 507, 772, 981, 1312
BstDEI	CTNAG	1 cut(s)	802
BstEII	GGTNACC	2 cut(s)	491, 929
BstF5I	GGATG	2 cut(s)	561, 1054
BstFNI	CGCG	1 cut(s)	130
BstH2I	RGCGCY	1 cut(s)	403
BstHHI	GCGC	2 cut(s)	132, 402
BstKTI	GATC	4 cut(s)	784, 838, 1184, 1200
BstMAI	GTCTC	1 cut(s)	850
BstMBI	GATC	4 cut(s)	781, 835, 1181, 1197
BstMWI	GCNNNNNNNGC	1 cut(s)	133
BstNI	CCWGG	4 cut(s)	312, 1027, 1175, 1344
BstNSI	RCATGY	1 cut(s)	950
BstPI	GGTNACC	2 cut(s)	491, 929
BstSCI	CCNGG	5 cut(s)	310, 843, 1025, 1173, 1342
BstSLI	GKGCMC	1 cut(s)	792
BstUI	CGCG	1 cut(s)	130
BstV1I	GCAGC	1 cut(s)	43
BstV2I	GAAGAC	1 cut(s)	206
BstX2I	RGATCY	1 cut(s)	1181
BstXI	CCANNNNNNTGG	2 cut(s)	832, 1359
BstYI	RGATCY	1 cut(s)	1181
Bsu15I	ATCGAT	1 cut(s)	727
BsuRI	GGCC	2 cut(s)	681, 722
BsuTUI	ATCGAT	1 cut(s)	727
BtsCI	GGATG	2 cut(s)	561, 1054
BtsI	GCAGTG	1 cut(s)	437
BtsIMutI	CAGTG	5 cut(s)	437, 820, 1192, 1200, 1330
BveI	ACCTGC	1 cut(s)	1139
Cac8I	GCNNGC	5 cut(s)	134, 507, 772, 981, 1312
CfoI	GCGC	2 cut(s)	132, 402
Cfr10I	RCCGGY	1 cut(s)	933
Cfr13I	GGNCC	1 cut(s)	720
ClaI	ATCGAT	1 cut(s)	727
CsiI	ACCWGGT	1 cut(s)	1025
Csp6I	GTAC	3 cut(s)	1015, 1128, 1218
CspAI	ACCGGT	1 cut(s)	933
CspCI	CAANNNNNGTGG	2 cut(s)	676, 711
CviAII	CATG	4 cut(s)	320, 826, 947, 1359
CviQI	GTAC	3 cut(s)	1015, 1128, 1218
DdeI	CTNAG	1 cut(s)	802
DinI	GGCGCC	1 cut(s)	401
DpnI	GATC	4 cut(s)	783, 837, 1183, 1199
DpnII	GATC	4 cut(s)	781, 835, 1181, 1197
DraI	TTTAAA	1 cut(s)	421
Eco130I	CCWWGG	1 cut(s)	372
Eco91I	GGTNACC	2 cut(s)	491, 929
EcoO65I	GGTNACC	2 cut(s)	491, 929
EcoRI	GAATTC	1 cut(s)	1347
EcoRII	CCWGG	4 cut(s)	310, 1025, 1173, 1342
EcoT14I	CCWWGG	1 cut(s)	372
EcoT22I	ATGCAT	5 cut(s)	304, 321, 831, 948, 1360
EgeI	GGCGCC	1 cut(s)	401
EheI	GGCGCC	1 cut(s)	401
ErhI	CCWWGG	1 cut(s)	372
FaeI	CATG	4 cut(s)	323, 829, 950, 1362
FaqI	GGGAC	2 cut(s)	182, 825
FatI	CATG	4 cut(s)	319, 825, 946, 1358
FauI	CCCGC	2 cut(s)	629, 1067
FblI	GTMKAC	2 cut(s)	457, 598
Fnu4HI	GCNGC	2 cut(s)	57, 128
FokI	GGATG	2 cut(s)	568, 1041
Fsp4HI	GCNGC	2 cut(s)	57, 128
FspBI	CTAG	1 cut(s)	785
GlaI	GCGC	2 cut(s)	131, 401
GluI	GCNGC	2 cut(s)	57, 128
GsaI	CCCAGC	1 cut(s)	721
HaeII	RGCGCY	1 cut(s)	403
HaeIII	GGCC	2 cut(s)	681, 722
HapII	CCGG	2 cut(s)	845, 934
HhaI	GCGC	2 cut(s)	132, 402
Hin1I	GRCGYC	1 cut(s)	400
Hin1II	CATG	4 cut(s)	323, 829, 950, 1362
Hin6I	GCGC	2 cut(s)	130, 400
HinP1I	GCGC	2 cut(s)	130, 400
HindIII	AAGCTT	1 cut(s)	1068
HinfI	GANTC	5 cut(s)	290, 587, 729, 1037, 1190
HpaII	CCGG	2 cut(s)	845, 934
HphI	GGTGA	2 cut(s)	1080, 1121
Hpy166II	GTNNAC	4 cut(s)	458, 599, 952, 1015
Hpy188I	TCNGA	5 cut(s)	442, 668, 805, 1080, 1157
Hpy188III	TCNNGA	5 cut(s)	547, 710, 853, 968, 1352
Hpy8I	GTNNAC	4 cut(s)	458, 599, 952, 1015
Hpy99I	CGWCG	1 cut(s)	696
HpyAV	CCTTC	7 cut(s)	159, 212, 217, 546, 875, 1172, 1262
HpyCH4III	ACNGT	2 cut(s)	1012, 1019
HpyCH4IV	ACGT	1 cut(s)	691
HpyF10VI	GCNNNNNNNGC	1 cut(s)	133
HpyF3I	CTNAG	1 cut(s)	802
HpySE526I	ACGT	1 cut(s)	691
Hsp92I	GRCGYC	1 cut(s)	400
Hsp92II	CATG	4 cut(s)	323, 829, 950, 1362
HspAI	GCGC	2 cut(s)	130, 400
KasI	GGCGCC	1 cut(s)	399
KpnI	GGTACC	1 cut(s)	1131
Kzo9I	GATC	4 cut(s)	781, 835, 1181, 1197
LmnI	GCTCC	1 cut(s)	698
Lsp1109I	GCAGC	1 cut(s)	43
LweI	GCATC	4 cut(s)	546, 910, 966, 1345
MabI	ACCWGGT	1 cut(s)	1025
MaeI	CTAG	1 cut(s)	785
MaeII	ACGT	1 cut(s)	691
MaeIII	GTNAC	5 cut(s)	491, 929, 1109, 1230, 1271
MalI	GATC	4 cut(s)	783, 837, 1183, 1199
MboI	GATC	4 cut(s)	781, 835, 1181, 1197
MboII	GAAGA	4 cut(s)	211, 260, 653, 1093
MfeI	CAATTG	1 cut(s)	1256
MflI	RGATCY	1 cut(s)	1181
MhlI	GDGCHC	1 cut(s)	792
MluCI	AATT	9 cut(s)	23, 181, 364, 511, 987, 1003, 1083, 1256, 1347
Mly113I	GGCGCC	1 cut(s)	400
MmeI	TCCRAC	2 cut(s)	646, 1135
MnlI	CCTC	6 cut(s)	196, 481, 1086, 1151, 1195, 1289
Mph1103I	ATGCAT	5 cut(s)	304, 321, 831, 948, 1360
MseI	TTAA	4 cut(s)	420, 795, 891, 924
MspA1I	CMGCKG	1 cut(s)	136
MspI	CCGG	2 cut(s)	845, 934
MspR9I	CCNGG	5 cut(s)	312, 845, 1027, 1175, 1344
MunI	CAATTG	1 cut(s)	1256
Mva1269I	GAATGC	4 cut(s)	302, 321, 340, 542
MvaI	CCWGG	4 cut(s)	312, 1027, 1175, 1344
MvnI	CGCG	1 cut(s)	130
MwoI	GCNNNNNNNGC	1 cut(s)	133
NarI	GGCGCC	1 cut(s)	400
NciI	CCSGG	1 cut(s)	845
NdeII	GATC	4 cut(s)	781, 835, 1181, 1197
NlaIII	CATG	4 cut(s)	323, 829, 950, 1362
NlaIV	GGNNCC	4 cut(s)	401, 700, 1129, 1183
NmuCI	GTSAC	2 cut(s)	1109, 1271
NsiI	ATGCAT	5 cut(s)	304, 321, 831, 948, 1360
NspI	RCATGY	1 cut(s)	950
PctI	GAATGC	4 cut(s)	302, 321, 340, 542
PfeI	GAWTC	5 cut(s)	290, 587, 729, 1037, 1190
PfoI	TCCNGGA	2 cut(s)	310, 1173
PinAI	ACCGGT	1 cut(s)	933
PkrI	GCNGC	2 cut(s)	58, 129
PluTI	GGCGCC	1 cut(s)	403
PshBI	ATTAAT	1 cut(s)	924
Psp6I	CCWGG	4 cut(s)	310, 1025, 1173, 1342
PspEI	GGTNACC	2 cut(s)	491, 929
PspFI	CCCAGC	1 cut(s)	717
PspGI	CCWGG	4 cut(s)	310, 1025, 1173, 1342
PspN4I	GGNNCC	4 cut(s)	401, 700, 1129, 1183
PspPI	GGNCC	1 cut(s)	720
PsuI	RGATCY	1 cut(s)	1181
RsaI	GTAC	3 cut(s)	1016, 1129, 1219
RsaNI	GTAC	3 cut(s)	1015, 1128, 1218
SaqAI	TTAA	4 cut(s)	420, 795, 891, 924
SatI	GCNGC	2 cut(s)	57, 128
Sau3AI	GATC	4 cut(s)	781, 835, 1181, 1197
Sau96I	GGNCC	1 cut(s)	720
ScrFI	CCNGG	5 cut(s)	312, 845, 1027, 1175, 1344
SduI	GDGCHC	1 cut(s)	792
SetI	ASST	9 cut(s)	196, 694, 867, 1031, 1072, 1133, 1219, 1273, 1345
SexAI	ACCWGGT	1 cut(s)	1025
SfaNI	GCATC	4 cut(s)	546, 910, 966, 1345
SfoI	GGCGCC	1 cut(s)	401
SmlI	CTYRAG	2 cut(s)	547, 853
SmoI	CTYRAG	2 cut(s)	547, 853
Sse9I	AATT	9 cut(s)	23, 181, 364, 511, 987, 1003, 1083, 1256, 1347
SsiI	CCGC	5 cut(s)	128, 136, 389, 636, 1060
SspDI	GGCGCC	1 cut(s)	399
SspI	AATATT	2 cut(s)	78, 214
SspMI	CTAG	1 cut(s)	785
StyD4I	CCNGG	5 cut(s)	310, 843, 1025, 1173, 1342
StyI	CCWWGG	1 cut(s)	372
TaaI	ACNGT	2 cut(s)	1012, 1019
TaiI	ACGT	1 cut(s)	694
TaqI	TCGA	2 cut(s)	727, 1200
TaqII	GACCGA	1 cut(s)	822
TasI	AATT	9 cut(s)	23, 181, 364, 511, 987, 1003, 1083, 1256, 1347
TatI	WGTACW	1 cut(s)	1014
TauI	GCSGC	1 cut(s)	130
TfiI	GAWTC	5 cut(s)	290, 587, 729, 1037, 1190
Tru1I	TTAA	4 cut(s)	420, 795, 891, 924
Tru9I	TTAA	4 cut(s)	420, 795, 891, 924
TscAI	CASTG	5 cut(s)	437, 820, 1192, 1200, 1337
TseFI	GTSAC	2 cut(s)	1109, 1271
TseI	GCWGC	1 cut(s)	56
Tsp45I	GTSAC	2 cut(s)	1109, 1271
TspDTI	ATGAA	8 cut(s)	17, 212, 336, 568, 579, 857, 1281, 1380
TspGWI	ACGGA	2 cut(s)	969, 1137
TspRI	CASTG	5 cut(s)	437, 820, 1192, 1200, 1337
VspI	ATTAAT	1 cut(s)	924
XapI	RAATTY	2 cut(s)	1003, 1347
XceI	RCATGY	1 cut(s)	950
XmiI	GTMKAC	2 cut(s)	457, 598
XspI	CTAG	1 cut(s)	785
Zsp2I	ATGCAT	5 cut(s)	304, 321, 831, 948, 1360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.