Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008475

Physical Location & Seq

Genomic Coordinates Reverse (-)

2253962 .. 2262775

Gene Structure 8814 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1006300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1389 bp

ATGAAGGGGGGATATAACTATAGATTGATTGGGAAACCAATCTCTCTCACTCTGGCTGCCCTTGTATTCACAACAATATTACTTTGGGCTTGGGAGAAAAATCCTTTTGCTGATACTCTACTATTGCAAGGCCAGCGGTTTACAATGCCTTCCATAGATTTTCTTGGGGACTTCTTAAATAATTCCTCAAAACCTATGAAGACAAATGGAAATGCTGAAGGGAAGGATTCATTTTTGAATACAGTAGAAGATAGAAAAGTAGTAGAGAATTTTGGCACTGCCCCACTAGATTCTATGTATGCATTCTCTCCTAGAATGCAAGAAAGTAATGGGATCGTGACATCTTTACAAACTAAAGTGTGTAATTATGCCAAGGGTAGATGGGTTGCAGATAGCACACGCCCTTTATATTCGGGGTTTAATTGTAAACAGTGGTTATCACAAATGTGGGCATGTAGGCTGACACAACGAACAGATTTTTCTTTTGAGGGCTACCGATGGAAGCCAGCAAATTGTGAAATGCCAGAGTTTGAACGCTCTGCATTCTTGAGAAGGATGCAGGACAAAACGATTGCTTTCATAGGAGATTCATTGGGCAGACAACAGTTCCAGTCTTTGATGTGTATGGCAACTGGTGGGGAAGAAAACCTAGAAGTTCAGAACGTTGGAAAGGAATATGGTCTTGTCAAACATCGTGGAGCCATTCGTCCTGATGGCTGGGCTTATCGATTCCCGAACACCAATACCACTATATTATATTATTGGTCAGCAAGCCTATCTGATCTAGTGCCCATTAACATCTCAGACCGAGCCAGTGATATTGCCATGCATTTGGATCGACCCCCGGCTTTCTTGAGACAATTCCTTCATCGGTTTGATGTGCTGGTTCTTAATACAGGGCATCATTGGAACAGGGGAAAGATTAATGGAAACCGGTGGGTAATGTATGTAGACGGAAAGCCCAATGAAGACAAGAAACGTGCAGAAATTGAGAATGCTAAGAATTTTACTGTGTACAGTATTTCCAGGTGGGTTGATTCTCAACTTCCATCACATCCTCGCCTAAAAGCTTTCTTTCGGACTATTTCACCAAGACATTTCTTCAATGGTGACTGGAATACTGGGGGTACCTGTGACAATACTAATCCGTTGGCTGGAGGAAGTGAAGTAGTCCAGGAAGGATCCAGTGATTCAGTAACTGAGGGTGCTTTCAAAGGTACAAAAATAAAGTTACTGGATATAACTGCTCTTTCTCAAATTAGAGATGAAGGTCACATATCTCACTATACTATTAGAGGAAAAGGAAATGCAGGCATAAATGATTGCTTACATTGGTGCTTACCGGGAATTCCAGATGCATGGAATGAACTCCTTGTTGCACAAATATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

463

Amino Acids

52.37

Weight (kDa)

8.77

Isoelectric Point (pI)

36.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	120 - 173	5.1e-15	PMR5 N terminal Domain
PC-Esterase	PF13839	174 - 460	1.3e-73	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G64020
fragaria_vesca	FvH4_1g24540 FvH4_1g24540 FvH4_1g24540 FvH4_1g24540 FvH4_1g24540 FvH4_1g24540
malus_domestica	MD01G1006300.v1.1 MD15G1134400.v1.1
prunus_persica	Prupe.4G274000_v2.0.a1 Prupe.4G274000_v2.0.a1
pyrus_communis	pycom15g12100
rosa_chinensis	RchiOBHm_Chr2g0121951
rosa_laevigata	RLG00000018636
rosa_multiflora	Rmu_sc0003880.1_g000021
rosa_roxburghii	Rroxscaffold_2G00122190
rosa_rugosa	Rorug02G0235000
rosa_samantha	Rh2AG292100 Rh2BG302300 Rh2CG281100 Rh2DG316300
rosa_wichuraiana	Rw2G023420 Rw2G023440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1127
AccB1I	GGYRCC	1 cut(s)	1127
AccI	GTMKAC	1 cut(s)	951
AciI	CCGC	1 cut(s)	136
AclI	AACGTT	1 cut(s)	663
AclWI	GGATC	4 cut(s)	341, 843, 1176, 1189
AcsI	RAATTY	3 cut(s)	268, 1003, 1347
AcuI	CTGAAG	1 cut(s)	237
AfaI	GTAC	3 cut(s)	1016, 1129, 1219
AfiI	CCNNNNNNNGG	1 cut(s)	1154
AgeI	ACCGGT	1 cut(s)	933
AgsI	TTSAA	4 cut(s)	238, 533, 1105, 1213
AjnI	CCWGG	2 cut(s)	1025, 1173
AleI	CACNNNNGTG	1 cut(s)	445
AluBI	AGCT	1 cut(s)	1070
AluI	AGCT	1 cut(s)	1070
Alw26I	GTCTC	1 cut(s)	850
AlwI	GGATC	4 cut(s)	341, 843, 1176, 1189
AlwNI	CAGNNNCTG	1 cut(s)	1199
AoxI	GGCC	1 cut(s)	130
ApeKI	GCWGC	1 cut(s)	56
ApoI	RAATTY	3 cut(s)	268, 1003, 1347
AseI	ATTAAT	1 cut(s)	924
AsiGI	ACCGGT	1 cut(s)	933
Asp718I	GGTACC	1 cut(s)	1127
AsuC2I	CCSGG	2 cut(s)	845, 1344
AsuHPI	GGTGA	2 cut(s)	1080, 1121
BaeGI	GKGCMC	1 cut(s)	792
BamHI	GGATCC	1 cut(s)	1181
BanI	GGYRCC	1 cut(s)	1127
BbsI	GAAGAC	2 cut(s)	206, 975
BbvI	GCAGC	1 cut(s)	43
BccI	CCATC	4 cut(s)	375, 492, 707, 1057
BcgI	CGANNNNNNTGC	2 cut(s)	818, 852
BciT130I	CCWGG	2 cut(s)	1027, 1175
BcnI	CCSGG	2 cut(s)	845, 1344
BcoDI	GTCTC	1 cut(s)	850
BfaI	CTAG	4 cut(s)	287, 312, 650, 785
BfmI	CTRYAG	1 cut(s)	19
BisI	GCNGC	1 cut(s)	57
BlsI	GCNGC	1 cut(s)	58
Bme1390I	CCNGG	4 cut(s)	845, 1027, 1175, 1344
BmiI	GGNNCC	3 cut(s)	700, 1129, 1183
BmrFI	CCNGG	4 cut(s)	845, 1027, 1175, 1344
BmrI	ACTGGG	1 cut(s)	1131
BmsI	GCATC	3 cut(s)	546, 910, 1345
BmuI	ACTGGG	1 cut(s)	1131
BpiI	GAAGAC	2 cut(s)	206, 975
BpmI	CTGGAG	1 cut(s)	1176
BpuEI	CTTGAG	2 cut(s)	568, 874
BpuMI	CCSGG	2 cut(s)	845, 1344
Bsa29I	ATCGAT	1 cut(s)	727
BsaJI	CCNNGG	2 cut(s)	372, 843
BsaWI	WCCGGW	1 cut(s)	933
Bsc4I	CCNNNNNNNGG	1 cut(s)	1154
Bse118I	RCCGGY	1 cut(s)	933
Bse1I	ACTGG	7 cut(s)	610, 637, 813, 1118, 1126, 1185, 1239
BseBI	CCWGG	2 cut(s)	1027, 1175
BseCI	ATCGAT	1 cut(s)	727
BseDI	CCNNGG	2 cut(s)	372, 843
BseGI	GGATG	2 cut(s)	561, 1054
BseLI	CCNNNNNNNGG	1 cut(s)	1154
BseMII	CTCAG	2 cut(s)	816, 1191
BseNI	ACTGG	7 cut(s)	610, 637, 813, 1118, 1126, 1185, 1239
BseSI	GKGCMC	1 cut(s)	792
BseXI	GCAGC	1 cut(s)	43
BseYI	CCCAGC	1 cut(s)	717
BsgI	GTGCAG	1 cut(s)	1002
BshFI	GGCC	1 cut(s)	132
BshNI	GGYRCC	1 cut(s)	1127
BshTI	ACCGGT	1 cut(s)	933
BshVI	ATCGAT	1 cut(s)	727
BsiSI	CCGG	3 cut(s)	845, 934, 1343
BslFI	GGGAC	1 cut(s)	182
BslI	CCNNNNNNNGG	1 cut(s)	1154
BsmAI	GTCTC	1 cut(s)	850
BsmFI	GGGAC	1 cut(s)	182
BsmI	GAATGC	4 cut(s)	302, 321, 542, 1000
BsnI	GGCC	1 cut(s)	132
Bsp1286I	GDGCHC	1 cut(s)	792
Bsp1407I	TGTACA	1 cut(s)	1014
Bsp143I	GATC	4 cut(s)	333, 781, 835, 1181
BspACI	CCGC	1 cut(s)	136
BspANI	GGCC	1 cut(s)	132
BspCNI	CTCAG	2 cut(s)	815, 1192
BspDI	ATCGAT	1 cut(s)	727
BspLI	GGNNCC	3 cut(s)	700, 1129, 1183
BspPI	GGATC	4 cut(s)	341, 843, 1176, 1189
BspT107I	GGYRCC	1 cut(s)	1127
BsrFI	RCCGGY	1 cut(s)	933
BsrGI	TGTACA	1 cut(s)	1014
BsrI	ACTGG	7 cut(s)	610, 637, 813, 1118, 1126, 1185, 1239
BssAI	RCCGGY	1 cut(s)	933
BssECI	CCNNGG	2 cut(s)	372, 843
BssMI	GATC	4 cut(s)	333, 781, 835, 1181
BssT1I	CCWWGG	1 cut(s)	372
Bst2UI	CCWGG	2 cut(s)	1027, 1175
Bst4CI	ACNGT	5 cut(s)	244, 432, 606, 1012, 1019
BstAUI	TGTACA	1 cut(s)	1014
BstC8I	GCNNGC	4 cut(s)	134, 507, 772, 1312
BstDEI	CTNAG	3 cut(s)	802, 999, 1200
BstF5I	GGATG	2 cut(s)	561, 1054
BstKTI	GATC	4 cut(s)	336, 784, 838, 1184
BstMAI	GTCTC	1 cut(s)	850
BstMBI	GATC	4 cut(s)	333, 781, 835, 1181
BstMWI	GCNNNNNNNGC	1 cut(s)	133
BstNI	CCWGG	2 cut(s)	1027, 1175
BstNSI	RCATGY	1 cut(s)	456
BstSCI	CCNGG	4 cut(s)	843, 1025, 1173, 1342
BstSFI	CTRYAG	1 cut(s)	19
BstSLI	GKGCMC	1 cut(s)	792
BstV1I	GCAGC	1 cut(s)	43
BstV2I	GAAGAC	2 cut(s)	206, 975
BstX2I	RGATCY	1 cut(s)	1181
BstXI	CCANNNNNNTGG	2 cut(s)	832, 1359
BstYI	RGATCY	1 cut(s)	1181
Bsu15I	ATCGAT	1 cut(s)	727
BsuRI	GGCC	1 cut(s)	132
BsuTUI	ATCGAT	1 cut(s)	727
BtsCI	GGATG	2 cut(s)	561, 1054
BtsI	GCAGTG	1 cut(s)	276
BtsIMutI	CAGTG	4 cut(s)	276, 437, 820, 1192
Cac8I	GCNNGC	4 cut(s)	134, 507, 772, 1312
CaiI	CAGNNNCTG	1 cut(s)	1199
Cfr10I	RCCGGY	1 cut(s)	933
ClaI	ATCGAT	1 cut(s)	727
Csp6I	GTAC	3 cut(s)	1015, 1128, 1218
CspAI	ACCGGT	1 cut(s)	933
CspCI	CAANNNNNGTGG	2 cut(s)	676, 711
CviAII	CATG	3 cut(s)	453, 826, 1359
CviQI	GTAC	3 cut(s)	1015, 1128, 1218
DdeI	CTNAG	3 cut(s)	802, 999, 1200
DpnI	GATC	4 cut(s)	335, 783, 837, 1183
DpnII	GATC	4 cut(s)	333, 781, 835, 1181
Eco130I	CCWWGG	1 cut(s)	372
Eco57I	CTGAAG	1 cut(s)	237
EcoRI	GAATTC	1 cut(s)	1347
EcoRII	CCWGG	2 cut(s)	1025, 1173
EcoT14I	CCWWGG	1 cut(s)	372
EcoT22I	ATGCAT	3 cut(s)	304, 831, 1360
ErhI	CCWWGG	1 cut(s)	372
FaeI	CATG	3 cut(s)	456, 829, 1362
FaqI	GGGAC	1 cut(s)	182
FatI	CATG	3 cut(s)	452, 825, 1358
FblI	GTMKAC	1 cut(s)	951
Fnu4HI	GCNGC	1 cut(s)	57
FokI	GGATG	2 cut(s)	568, 1041
Fsp4HI	GCNGC	1 cut(s)	57
FspBI	CTAG	4 cut(s)	287, 312, 650, 785
GluI	GCNGC	1 cut(s)	57
GsaI	CCCAGC	1 cut(s)	721
GsuI	CTGGAG	1 cut(s)	1176
HaeIII	GGCC	1 cut(s)	132
HapII	CCGG	3 cut(s)	845, 934, 1343
Hin1II	CATG	3 cut(s)	456, 829, 1362
HindIII	AAGCTT	1 cut(s)	1068
HinfI	GANTC	6 cut(s)	227, 290, 587, 729, 1037, 1190
HpaII	CCGG	3 cut(s)	845, 934, 1343
HphI	GGTGA	2 cut(s)	1080, 1121
Hpy166II	GTNNAC	4 cut(s)	141, 428, 952, 1015
Hpy188I	TCNGA	4 cut(s)	660, 781, 805, 1080
Hpy188III	TCNNGA	6 cut(s)	337, 547, 710, 733, 853, 1352
Hpy8I	GTNNAC	4 cut(s)	141, 428, 952, 1015
HpyAV	CCTTC	7 cut(s)	159, 212, 217, 546, 875, 1172, 1262
HpyCH4III	ACNGT	5 cut(s)	244, 432, 606, 1012, 1019
HpyCH4IV	ACGT	2 cut(s)	663, 979
HpyF10VI	GCNNNNNNNGC	1 cut(s)	133
HpyF3I	CTNAG	3 cut(s)	802, 999, 1200
HpySE526I	ACGT	2 cut(s)	663, 979
Hsp92II	CATG	3 cut(s)	456, 829, 1362
KpnI	GGTACC	1 cut(s)	1131
Kzo9I	GATC	4 cut(s)	333, 781, 835, 1181
LmnI	GCTCC	1 cut(s)	698
Lsp1109I	GCAGC	1 cut(s)	43
LweI	GCATC	3 cut(s)	546, 910, 1345
MaeI	CTAG	4 cut(s)	287, 312, 650, 785
MaeII	ACGT	2 cut(s)	663, 979
MaeIII	GTNAC	6 cut(s)	337, 1109, 1133, 1195, 1230, 1271
MalI	GATC	4 cut(s)	335, 783, 837, 1183
MboI	GATC	4 cut(s)	333, 781, 835, 1181
MboII	GAAGA	5 cut(s)	211, 260, 653, 980, 1093
MflI	RGATCY	1 cut(s)	1181
MhlI	GDGCHC	1 cut(s)	792
MmeI	TCCRAC	1 cut(s)	646
MnlI	CCTC	6 cut(s)	196, 481, 1068, 1151, 1195, 1289
Mph1103I	ATGCAT	3 cut(s)	304, 831, 1360
MseI	TTAA	5 cut(s)	176, 420, 795, 891, 924
MslI	CAYNNNNRTG	1 cut(s)	445
MspA1I	CMGCKG	1 cut(s)	136
MspI	CCGG	3 cut(s)	845, 934, 1343
MspR9I	CCNGG	4 cut(s)	845, 1027, 1175, 1344
Mva1269I	GAATGC	4 cut(s)	302, 321, 542, 1000
MvaI	CCWGG	2 cut(s)	1027, 1175
MwoI	GCNNNNNNNGC	1 cut(s)	133
NciI	CCSGG	2 cut(s)	845, 1344
NdeII	GATC	4 cut(s)	333, 781, 835, 1181
NlaIII	CATG	3 cut(s)	456, 829, 1362
NlaIV	GGNNCC	3 cut(s)	700, 1129, 1183
NmuCI	GTSAC	4 cut(s)	337, 1109, 1133, 1271
NsiI	ATGCAT	3 cut(s)	304, 831, 1360
NspI	RCATGY	1 cut(s)	456
OliI	CACNNNNGTG	1 cut(s)	445
PctI	GAATGC	4 cut(s)	302, 321, 542, 1000
PfeI	GAWTC	6 cut(s)	227, 290, 587, 729, 1037, 1190
PfoI	TCCNGGA	1 cut(s)	1173
PinAI	ACCGGT	1 cut(s)	933
PkrI	GCNGC	1 cut(s)	58
PshBI	ATTAAT	1 cut(s)	924
Psp1406I	AACGTT	1 cut(s)	663
Psp6I	CCWGG	2 cut(s)	1025, 1173
PspFI	CCCAGC	1 cut(s)	717
PspGI	CCWGG	2 cut(s)	1025, 1173
PspN4I	GGNNCC	3 cut(s)	700, 1129, 1183
PstNI	CAGNNNCTG	1 cut(s)	1199
PsuI	RGATCY	1 cut(s)	1181
RsaI	GTAC	3 cut(s)	1016, 1129, 1219
RsaNI	GTAC	3 cut(s)	1015, 1128, 1218
RseI	CAYNNNNRTG	1 cut(s)	445
SaqAI	TTAA	5 cut(s)	176, 420, 795, 891, 924
SatI	GCNGC	1 cut(s)	57
Sau3AI	GATC	4 cut(s)	333, 781, 835, 1181
ScrFI	CCNGG	4 cut(s)	845, 1027, 1175, 1344
SduI	GDGCHC	1 cut(s)	792
SetI	ASST	9 cut(s)	196, 651, 666, 982, 1031, 1072, 1133, 1219, 1273
SfaNI	GCATC	3 cut(s)	546, 910, 1345
SfcI	CTRYAG	1 cut(s)	19
SmiMI	CAYNNNNRTG	1 cut(s)	445
SmlI	CTYRAG	2 cut(s)	547, 853
SmoI	CTYRAG	2 cut(s)	547, 853
SsiI	CCGC	1 cut(s)	136
SspI	AATATT	1 cut(s)	78
SspMI	CTAG	4 cut(s)	287, 312, 650, 785
StyD4I	CCNGG	4 cut(s)	843, 1025, 1173, 1342
StyI	CCWWGG	1 cut(s)	372
TaaI	ACNGT	5 cut(s)	244, 432, 606, 1012, 1019
TaiI	ACGT	2 cut(s)	666, 982
TaqI	TCGA	2 cut(s)	727, 838
TaqII	GACCGA	1 cut(s)	822
TatI	WGTACW	1 cut(s)	1014
TfiI	GAWTC	6 cut(s)	227, 290, 587, 729, 1037, 1190
Tru1I	TTAA	5 cut(s)	176, 420, 795, 891, 924
Tru9I	TTAA	5 cut(s)	176, 420, 795, 891, 924
TscAI	CASTG	4 cut(s)	283, 437, 820, 1192
TseFI	GTSAC	4 cut(s)	337, 1109, 1133, 1271
TseI	GCWGC	1 cut(s)	56
Tsp45I	GTSAC	4 cut(s)	337, 1109, 1133, 1271
TspDTI	ATGAA	9 cut(s)	17, 212, 219, 568, 579, 857, 981, 1281, 1380
TspGWI	ACGGA	2 cut(s)	969, 1137
TspRI	CASTG	4 cut(s)	283, 437, 820, 1192
VspI	ATTAAT	1 cut(s)	924
XapI	RAATTY	3 cut(s)	268, 1003, 1347
XceI	RCATGY	1 cut(s)	456
XmiI	GTMKAC	1 cut(s)	951
XspI	CTAG	4 cut(s)	287, 312, 650, 785
Zsp2I	ATGCAT	3 cut(s)	304, 831, 1360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.