AT5G64600

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015813
Physical Location & Seq
Forward (+)
25824997 .. 25828185
3189 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64600.1

Sequence Viewer

Length: 1569 bp
ATGGTGAAACACAGAAACTCATCTCGCTCGATAATCTCATATTCTTCTTCAATAGCAAGATTCTTCTCTAGAAAAGCTATTTCTCTCTACTTGATCTTCGTCTTTGCTTTTACCATCTGGGTTCTCGTCTTCAGCTCCAGAAACATTCAAACCGATGATGACCACACCAAACATCAACAACAACATCATCGGGATCTAATCGATTCAGAATCATTTCCGCCACCGTATTTGCCTCCTAGGAAGAATTTGCAGAAACCGTATGAAAATACTCAACTTTGGACTCCTCCTTTCAGCTTTGGGTTGCATCCATGTGTCAAACCTACTCCGAAATACAAAGAATTTTCAGAATCAGATCATTATATAACAGTGAAAAGTAATGGTGGACTAAATCAAATGCGTACTGGTATAGCAGATATAGTGGCTGTTGCGCACATCATGAATGCAACTTTAGTCATTCCTGAGCTGGATAAGCGATCGTTTTGGCAAGATTCAAGTGTTTTTTCAGATATTTTTGACGAGGAACAATTCATTAAATCATTGCGAAGAGATGTCAAGGTTATTAAAAAGCTCCCAAAGGAAGTGGAATCTCTACCTAGAGCAAGGAAGCATTTCACTTCTTGGTCTAGTGTTGGATATTATGAAGAAATGACTCACTTGTGGAAGGAGTACAAGGTCATCCATGTCGCAAAATCAGATTCTCGCCTTGCAAATAATGACCTGCCTATCGACGTTCAAAGACTGAGATGTCGTGTACTATATCGTGGTCTCTGCTTCTCTCCTGCAATTGAAAGCCTTGGACAGAAGCTGGTTGAGAGACTCAAGTCACGTGCTGGGAGATATATTGCATTGCACCTGAGATATGAGAAAGATATGTTGGCTTTCACTGGTTGCACCTATGGTCTCACTGATGCTGAATCCGAAGAACTGAGAGTAATGCGGGAAAGTACAAGCCATTGGAAGATCAAAAGTATAAATTCAACAGAGCAGAGAGAGGAAGGCCTTTGTCCATTGACTCCAAAAGAAGTGGGAATATTTCTGAAAGGTCTCGGATATTCTCAGTCAACAGTCATATATATTGCAGCAGGGGAAATCTATGGGGGTGATGATAGACTCTCTGAGCTTAAGTCGCGCTTCCCAAATCTGGTTTTTAAGGAAACGCTTGCTGGGAACGAAGAGTTAAAAGGTTTCACTGGCCATGCGACTAAAACGGCTGCTCTTGATTACATAATTTCTGTTGAGAGTGATGTGTTTGTTCCTTCACATTCTGGAAACATGGCAAGAGCAGTTGAAGGTCACCGCAGATTTCTAGGGCATCGCAGGACTATCACTCCCGACAGGAAAGGACTAGTGAAACTCTTCGTTAAGATGGAGAGAGGACAGCTAAAAGAAGGACCAAAGTTGTCCAATTTTGTGAATCAAATGCATAAAGACAGACAAGGTGCACCGAGGAGAAGGAAAGGACCAACGCAGGGGATCAAAGGACGTGCACGGTTTAGAACTGAAGAAGCCTTTTATGAGAATCCATATCCAGAGTGTATTTGCAGTTCAAAGGAGCACAAAGAACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

522

Amino Acids

60.06

Weight (kDa)

9.48

Isoelectric Point (pI)

43.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 120 - 435 1.3e-104 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64600
fragaria_vesca FvH4_1g24200 FvH4_1g24200 FvH4_1g24200
rosa_chinensis RchiOBHm_Chr2g0120631 RchiOBHm_Chr2g0120641
rosa_laevigata RLG00000018563
rosa_multiflora Rmu_sc0001844.1_g000005
rosa_roxburghii Rroxscaffold_2G00123270
rosa_rugosa Rorug02G0230000
rosa_samantha Rh2AG286000 Rh2BG297800 Rh2CG275500 Rh2DG311500
rosa_wichuraiana Rw2G022970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 744
Acc16I TGCGCA 1 cut(s) 429
Acc36I ACCTGC 1 cut(s) 726
AccII CGCG 1 cut(s) 1129
AciI CCGC 3 cut(s) 218, 937, 1297
AclWI GGATC 2 cut(s) 201, 1481
AcoI YGGCCR 1 cut(s) 1192
AcsI RAATTY 3 cut(s) 244, 338, 973
AcuI CTGAAG 2 cut(s) 115, 1521
AcvI CACGTG 1 cut(s) 827
AfaI GTAC 4 cut(s) 400, 668, 753, 946
AfiI CCNNNNNNNGG 2 cut(s) 1141, 1469
AflII CTTAAG 1 cut(s) 1121
AgsI TTSAA 8 cut(s) 51, 149, 492, 734, 788, 978, 1289, 1548
AhlI ACTAGT 1 cut(s) 1345
AjiI CACGTC 1 cut(s) 1484
AjuI GAANNNNNNNTTGG 2 cut(s) 857, 889
AluBI AGCT 8 cut(s) 77, 135, 294, 463, 568, 805, 1120, 1381
AluI AGCT 8 cut(s) 77, 135, 294, 463, 568, 805, 1120, 1381
Alw21I GWGCWC 3 cut(s) 1444, 1489, 1557
Alw26I GTCTC 4 cut(s) 770, 808, 905, 1049
Alw44I GTGCAC 2 cut(s) 1440, 1485
AlwI GGATC 2 cut(s) 201, 1481
AlwNI CAGNNNCTG 1 cut(s) 805
AoxI GGCC 2 cut(s) 997, 1192
ApaLI GTGCAC 2 cut(s) 1440, 1485
ApeKI GCWGC 2 cut(s) 1079, 1211
ApoI RAATTY 3 cut(s) 244, 338, 973
Asp700I GAANNNNTTC 3 cut(s) 213, 608, 1355
AspA2I CCTAGG 1 cut(s) 236
AspLEI GCGC 2 cut(s) 430, 1131
AspS9I GGNCC 2 cut(s) 1391, 1460
AsuHPI GGTGA 3 cut(s) 16, 1112, 1286
AvaII GGWCC 2 cut(s) 1391, 1460
AvrII CCTAGG 1 cut(s) 236
BaeGI GKGCMC 2 cut(s) 1444, 1489
BalI TGGCCA 1 cut(s) 1194
BbrPI CACGTG 1 cut(s) 827
BbsI GAAGAC 1 cut(s) 121
Bbv12I GWGCWC 3 cut(s) 1444, 1489, 1557
BbvI GCAGC 2 cut(s) 1091, 1198
BccI CCATC 2 cut(s) 122, 1360
BceAI ACGGC 1 cut(s) 1224
BcoDI GTCTC 4 cut(s) 770, 808, 905, 1049
BcuI ACTAGT 1 cut(s) 1345
BfaI CTAG 6 cut(s) 69, 237, 594, 624, 1307, 1346
BfrI CTTAAG 1 cut(s) 1121
BfuAI ACCTGC 1 cut(s) 726
BisI GCNGC 2 cut(s) 1080, 1212
BlnI CCTAGG 1 cut(s) 236
BlsI GCNGC 2 cut(s) 1081, 1213
Bme18I GGWCC 2 cut(s) 1391, 1460
BmgBI CACGTC 1 cut(s) 1484
BmgT120I GGNCC 2 cut(s) 1391, 1460
BmsI GCATC 3 cut(s) 313, 898, 1321
BoxI GACNNNNGTC 1 cut(s) 820
BpiI GAAGAC 1 cut(s) 121
BpmI CTGGAG 1 cut(s) 121
Bpu10I CCTNAGC 1 cut(s) 459
BpuEI CTTGAG 1 cut(s) 803
Bsa29I ATCGAT 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 827
BsaI GGTCTC 3 cut(s) 770, 905, 1049
BsaJI CCNNGG 3 cut(s) 236, 793, 1445
BsaXI ACNNNNNCTCC 2 cut(s) 1312, 1342
Bsc4I CCNNNNNNNGG 2 cut(s) 1141, 1469
Bse1I ACTGG 3 cut(s) 406, 889, 1195
Bse3DI GCAATG 2 cut(s) 536, 845
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 3 cut(s) 236, 793, 1445
BseGI GGATG 2 cut(s) 304, 675
BseLI CCNNNNNNNGG 2 cut(s) 1141, 1469
BseMI GCAATG 2 cut(s) 536, 845
BseMII CTCAG 6 cut(s) 450, 731, 845, 917, 1070, 1107
BseNI ACTGG 3 cut(s) 406, 889, 1195
BseRI GAGGAG 2 cut(s) 273, 1462
BseSI GKGCMC 2 cut(s) 1444, 1489
BseXI GCAGC 2 cut(s) 1091, 1198
BseYI CCCAGC 2 cut(s) 830, 1163
Bsh1236I CGCG 1 cut(s) 1129
Bsh1285I CGRYCG 1 cut(s) 476
BshFI GGCC 2 cut(s) 999, 1194
BshVI ATCGAT 1 cut(s) 201
BsiEI CGRYCG 1 cut(s) 476
BsiHKAI GWGCWC 3 cut(s) 1444, 1489, 1557
BslI CCNNNNNNNGG 2 cut(s) 1141, 1469
BsmAI GTCTC 4 cut(s) 770, 808, 905, 1049
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 2 cut(s) 999, 1194
Bso31I GGTCTC 3 cut(s) 770, 905, 1049
Bsp1286I GDGCHC 3 cut(s) 1444, 1489, 1557
Bsp143I GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
BspACI CCGC 3 cut(s) 218, 937, 1297
BspANI GGCC 2 cut(s) 999, 1194
BspCNI CTCAG 6 cut(s) 451, 732, 846, 918, 1069, 1108
BspDI ATCGAT 1 cut(s) 201
BspFNI CGCG 1 cut(s) 1129
BspHI TCATGA 1 cut(s) 435
BspMI ACCTGC 1 cut(s) 726
BspPI GGATC 2 cut(s) 201, 1481
BspTI CTTAAG 1 cut(s) 1121
BspTNI GGTCTC 3 cut(s) 770, 905, 1049
BsrDI GCAATG 2 cut(s) 536, 845
BsrI ACTGG 3 cut(s) 406, 889, 1195
BssECI CCNNGG 3 cut(s) 236, 793, 1445
BssMI GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
BssT1I CCWWGG 2 cut(s) 236, 793
Bst4CI ACNGT 5 cut(s) 225, 258, 367, 1066, 1491
Bst6I CTCTTC 3 cut(s) 538, 1167, 1361
BstAFI CTTAAG 1 cut(s) 1121
BstBAI YACGTR 1 cut(s) 827
BstC8I GCNNGC 1 cut(s) 1161
BstDEI CTNAG 6 cut(s) 459, 740, 854, 926, 1056, 1116
BstEII GGTNACC 1 cut(s) 1292
BstF5I GGATG 2 cut(s) 304, 675
BstFNI CGCG 1 cut(s) 1129
BstHHI GCGC 2 cut(s) 430, 1131
BstKTI GATC 6 cut(s) 96, 196, 355, 476, 963, 1476
BstMAI GTCTC 4 cut(s) 770, 808, 905, 1049
BstMBI GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
BstMCI CGRYCG 1 cut(s) 476
BstMWI GCNNNNNNNGC 2 cut(s) 469, 1126
BstPAI GACNNNNGTC 1 cut(s) 820
BstPI GGTNACC 1 cut(s) 1292
BstSLI GKGCMC 2 cut(s) 1444, 1489
BstUI CGCG 1 cut(s) 1129
BstV1I GCAGC 2 cut(s) 1091, 1198
BstV2I GAAGAC 1 cut(s) 121
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 201
BsuRI GGCC 2 cut(s) 999, 1194
BsuTUI ATCGAT 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 1298
BtrI CACGTC 1 cut(s) 1484
BtsCI GGATG 2 cut(s) 304, 675
BtsIMutI CAGTG 4 cut(s) 372, 882, 903, 1188
BveI ACCTGC 1 cut(s) 726
Cac8I GCNNGC 1 cut(s) 1161
CaiI CAGNNNCTG 1 cut(s) 805
CciI TCATGA 1 cut(s) 435
CfoI GCGC 2 cut(s) 430, 1131
Cfr13I GGNCC 2 cut(s) 1391, 1460
ClaI ATCGAT 1 cut(s) 201
Csp6I GTAC 4 cut(s) 399, 667, 752, 945
CspCI CAANNNNNGTGG 6 cut(s) 210, 245, 561, 596, 1005, 1040
CviAII CATG 5 cut(s) 309, 436, 680, 1196, 1273
CviQI GTAC 4 cut(s) 399, 667, 752, 945
DdeI CTNAG 6 cut(s) 459, 740, 854, 926, 1056, 1116
DpnI GATC 6 cut(s) 95, 195, 354, 475, 962, 1475
DpnII GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
DrdI GACNNNNNNGTC 1 cut(s) 744
DseDI GACNNNNNNGTC 1 cut(s) 744
EaeI YGGCCR 1 cut(s) 1192
Eam1104I CTCTTC 3 cut(s) 538, 1167, 1361
EarI CTCTTC 3 cut(s) 538, 1167, 1361
EciI GGCGGA 1 cut(s) 207
Eco130I CCWWGG 2 cut(s) 236, 793
Eco147I AGGCCT 1 cut(s) 999
Eco31I GGTCTC 3 cut(s) 770, 905, 1049
Eco47I GGWCC 2 cut(s) 1391, 1460
Eco57I CTGAAG 2 cut(s) 115, 1521
Eco72I CACGTG 1 cut(s) 827
Eco91I GGTNACC 1 cut(s) 1292
EcoO65I GGTNACC 1 cut(s) 1292
EcoT14I CCWWGG 2 cut(s) 236, 793
EcoT22I ATGCAT 1 cut(s) 1425
ErhI CCWWGG 2 cut(s) 236, 793
FaeI CATG 5 cut(s) 312, 439, 683, 1199, 1276
FalI AAGNNNNNCTT 2 cut(s) 1115, 1147
FatI CATG 5 cut(s) 308, 435, 679, 1195, 1272
FauI CCCGC 1 cut(s) 930
Fnu4HI GCNGC 2 cut(s) 1080, 1212
FokI GGATG 2 cut(s) 291, 662
Fsp4HI GCNGC 2 cut(s) 1080, 1212
FspBI CTAG 6 cut(s) 69, 237, 594, 624, 1307, 1346
FspI TGCGCA 1 cut(s) 429
GlaI GCGC 2 cut(s) 429, 1130
GluI GCNGC 2 cut(s) 1080, 1212
GsaI CCCAGC 2 cut(s) 834, 1167
GsuI CTGGAG 1 cut(s) 121
HaeIII GGCC 2 cut(s) 999, 1194
HhaI GCGC 2 cut(s) 430, 1131
Hin1II CATG 5 cut(s) 312, 439, 683, 1199, 1276
Hin6I GCGC 2 cut(s) 428, 1129
HinP1I GCGC 2 cut(s) 428, 1129
HincII GTYRAC 1 cut(s) 1062
HindII GTYRAC 1 cut(s) 1062
HphI GGTGA 3 cut(s) 16, 1112, 1286
Hpy166II GTNNAC 5 cut(s) 383, 752, 1062, 1442, 1487
Hpy188III TCNNGA 9 cut(s) 69, 138, 191, 436, 458, 1217, 1266, 1331, 1529
Hpy8I GTNNAC 5 cut(s) 383, 752, 1062, 1442, 1487
Hpy99I CGWCG 1 cut(s) 731
HpyAV CCTTC 6 cut(s) 655, 989, 1266, 1283, 1382, 1446
HpyCH4III ACNGT 5 cut(s) 225, 258, 367, 1066, 1491
HpyCH4IV ACGT 3 cut(s) 729, 826, 1483
HpyF10VI GCNNNNNNNGC 2 cut(s) 469, 1126
HpyF3I CTNAG 6 cut(s) 459, 740, 854, 926, 1056, 1116
HpySE526I ACGT 3 cut(s) 729, 826, 1483
Hsp92II CATG 5 cut(s) 312, 439, 683, 1199, 1276
HspAI GCGC 2 cut(s) 428, 1129
Kzo9I GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
LmnI GCTCC 3 cut(s) 140, 573, 1552
Lsp1109I GCAGC 2 cut(s) 1091, 1198
LweI GCATC 3 cut(s) 313, 898, 1321
MaeI CTAG 6 cut(s) 69, 237, 594, 624, 1307, 1346
MaeII ACGT 3 cut(s) 729, 826, 1483
MaeIII GTNAC 2 cut(s) 822, 1292
MalI GATC 6 cut(s) 95, 195, 354, 475, 962, 1475
MboI GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
MfeI CAATTG 1 cut(s) 783
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 3 cut(s) 1444, 1489, 1557
MlsI TGGCCA 1 cut(s) 1194
MluCI AATT 7 cut(s) 244, 338, 524, 783, 973, 1227, 1405
MluNI TGGCCA 1 cut(s) 1194
MlyI GAGTC 5 cut(s) 274, 643, 810, 1006, 1104
MmeI TCCRAC 1 cut(s) 610
MnlI CCTC 6 cut(s) 243, 294, 511, 985, 1367, 1440
Mox20I TGGCCA 1 cut(s) 1194
Mph1103I ATGCAT 1 cut(s) 1425
MroXI GAANNNNTTC 3 cut(s) 213, 608, 1355
MscI TGGCCA 1 cut(s) 1194
MseI TTAA 6 cut(s) 531, 561, 1122, 1149, 1178, 1362
MslI CAYNNNNRTG 1 cut(s) 309
Msp20I TGGCCA 1 cut(s) 1194
MspCI CTTAAG 1 cut(s) 1121
MunI CAATTG 1 cut(s) 783
Mva1269I GAATGC 1 cut(s) 445
MvnI CGCG 1 cut(s) 1129
MwoI GCNNNNNNNGC 2 cut(s) 469, 1126
NdeII GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
NlaIII CATG 5 cut(s) 312, 439, 683, 1199, 1276
NmuCI GTSAC 2 cut(s) 822, 1292
NsbI TGCGCA 1 cut(s) 429
NsiI ATGCAT 1 cut(s) 1425
PagI TCATGA 1 cut(s) 435
PceI AGGCCT 1 cut(s) 999
PctI GAATGC 1 cut(s) 445
PdmI GAANNNNTTC 3 cut(s) 213, 608, 1355
PkrI GCNGC 2 cut(s) 1081, 1213
Ple19I CGATCG 1 cut(s) 476
PleI GAGTC 5 cut(s) 274, 643, 810, 1006, 1104
PmaCI CACGTG 1 cut(s) 827
PmlI CACGTG 1 cut(s) 827
PpsI GAGTC 5 cut(s) 274, 643, 810, 1006, 1104
Ppu21I YACGTR 1 cut(s) 827
PshAI GACNNNNGTC 1 cut(s) 820
PspCI CACGTG 1 cut(s) 827
PspEI GGTNACC 1 cut(s) 1292
PspFI CCCAGC 2 cut(s) 830, 1163
PspPI GGNCC 2 cut(s) 1391, 1460
PsrI GAACNNNNNNTAC 2 cut(s) 1528, 1560
PstNI CAGNNNCTG 1 cut(s) 805
PsuI RGATCY 1 cut(s) 193
PvuI CGATCG 1 cut(s) 476
RsaI GTAC 4 cut(s) 400, 668, 753, 946
RsaNI GTAC 4 cut(s) 399, 667, 752, 945
RseI CAYNNNNRTG 1 cut(s) 309
SaqAI TTAA 6 cut(s) 531, 561, 1122, 1149, 1178, 1362
SatI GCNGC 2 cut(s) 1080, 1212
Sau3AI GATC 6 cut(s) 93, 193, 352, 473, 960, 1473
Sau96I GGNCC 2 cut(s) 1391, 1460
SchI GAGTC 5 cut(s) 274, 643, 810, 1006, 1104
SduI GDGCHC 3 cut(s) 1444, 1489, 1557
SfaNI GCATC 3 cut(s) 313, 898, 1321
SinI GGWCC 2 cut(s) 1391, 1460
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 2 cut(s) 818, 1121
SmoI CTYRAG 2 cut(s) 818, 1121
SpeI ACTAGT 1 cut(s) 1345
Sse9I AATT 7 cut(s) 244, 338, 524, 783, 973, 1227, 1405
SseBI AGGCCT 1 cut(s) 999
SsiI CCGC 3 cut(s) 218, 937, 1297
SspI AATATT 1 cut(s) 1032
SspMI CTAG 6 cut(s) 69, 237, 594, 624, 1307, 1346
StuI AGGCCT 1 cut(s) 999
StyI CCWWGG 2 cut(s) 236, 793
TaaI ACNGT 5 cut(s) 225, 258, 367, 1066, 1491
TaiI ACGT 3 cut(s) 732, 829, 1486
TaqI TCGA 3 cut(s) 29, 201, 726
TasI AATT 7 cut(s) 244, 338, 524, 783, 973, 1227, 1405
TatI WGTACW 3 cut(s) 666, 751, 944
Tru1I TTAA 6 cut(s) 531, 561, 1122, 1149, 1178, 1362
Tru9I TTAA 6 cut(s) 531, 561, 1122, 1149, 1178, 1362
TscAI CASTG 4 cut(s) 372, 889, 910, 1195
TseFI GTSAC 2 cut(s) 822, 1292
TseI GCWGC 2 cut(s) 1079, 1211
Tsp45I GTSAC 2 cut(s) 822, 1292
TspDTI ATGAA 4 cut(s) 276, 452, 517, 654
TspRI CASTG 4 cut(s) 372, 889, 910, 1195
Vha464I CTTAAG 1 cut(s) 1121
VneI GTGCAC 2 cut(s) 1440, 1485
VpaK11BI GGWCC 2 cut(s) 1391, 1460
XapI RAATTY 3 cut(s) 244, 338, 973
XbaI TCTAGA 1 cut(s) 68
XmaJI CCTAGG 1 cut(s) 236
XmnI GAANNNNTTC 3 cut(s) 213, 608, 1355
XspI CTAG 6 cut(s) 69, 237, 594, 624, 1307, 1346
Zsp2I ATGCAT 1 cut(s) 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.