RchiOBHm_Chr2g0120641

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015813
Physical Location & Seq
Forward (+)
33423347 .. 33426080
2734 bp
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UTR
Exon/CDS
Intron
PRQ49324

Sequence Viewer

Length: 696 bp
ATGTTGTCTTTTACTGGTTGTTCTTATGGTTTGACTGATGAGGAATCCGAGGAACTAAGAATAATGAGGGAGAACACAAATCACTGGAAAGTTAAAAAGATTAACTCTACTGAGCAGAGACTAGGTGGCTTCTGTCCACTAACTCCCAAGGAGATTGGCATATTTCTTCAAGCTCTCGGTTATCCACCATCATCGTTAATATATATTGCAGCCGGGGAGATCTATGGTGGTAGTACTCACCTTTTGGAGCTCACATCCCGTTTTCCAAATATAGTTTTCAAGGAAACACTTGCGACCCAGGAGGAACTAAGGGCATTTACTAATCATGCATCTCAAACTGCAGCTCTTGATTATGTGATCTCAGTTGAAAGTGATGTGTTTGTCCCATCATATTCTGGTAACATGGCAAGAGCTGTTGAGGGCCAACGCAGATTCTTAGGTCACCGCAAAACGATCACTCCAGATAGGAAAGGACTTGTTGAAGTTTTTGATATGCTGGAAAATGGACAACAAACAGAAGGATCTTCGCTGTCAGATATTATACAGACCATGCACAAAAATAGGAAAGGAGCTCCAAGGAGGAGAAGAGGGCCACTGCCAGAAACGAAAGGTCGAGCACGATTGAGAACTGAAGAATCTTTCTATGAAAACCCTTACCCTGAGTGTATATGTGCTTCGAAAAAGTACTTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

231

Amino Acids

26.03

Weight (kDa)

8.3

Isoelectric Point (pI)

52.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 3 - 145 2.4e-36 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64600
fragaria_vesca FvH4_1g24200 FvH4_1g24200 FvH4_1g24200
rosa_chinensis RchiOBHm_Chr2g0120631 RchiOBHm_Chr2g0120641
rosa_laevigata RLG00000018563
rosa_multiflora Rmu_sc0001844.1_g000005
rosa_roxburghii Rroxscaffold_2G00123270
rosa_rugosa Rorug02G0230000
rosa_samantha Rh2AG286000 Rh2BG297800 Rh2CG275500 Rh2DG311500
rosa_wichuraiana Rw2G022970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 445
AclWI GGATC 1 cut(s) 529
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 2 cut(s) 235, 686
AgsI TTSAA 4 cut(s) 170, 280, 368, 482
AjnI CCWGG 1 cut(s) 297
AjuI GAANNNNNNNTTGG 1 cut(s) 671
AluBI AGCT 5 cut(s) 173, 250, 344, 413, 572
AluI AGCT 5 cut(s) 173, 250, 344, 413, 572
Alw21I GWGCWC 3 cut(s) 252, 574, 619
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 1 cut(s) 529
AoxI GGCC 2 cut(s) 421, 590
ApeKI GCWGC 2 cut(s) 209, 341
AspS9I GGNCC 2 cut(s) 421, 590
AsuC2I CCSGG 1 cut(s) 214
AsuHPI GGTGA 2 cut(s) 230, 434
AsuII TTCGAA 1 cut(s) 677
BanII GRGCYC 2 cut(s) 252, 574
BarI GAAGNNNNNNTAC 2 cut(s) 658, 690
Bbv12I GWGCWC 3 cut(s) 252, 574, 619
BbvI GCAGC 2 cut(s) 221, 353
BccI CCATC 2 cut(s) 196, 394
BciT130I CCWGG 1 cut(s) 299
BcnI CCSGG 1 cut(s) 214
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 122
BfmI CTRYAG 1 cut(s) 339
BglII AGATCT 1 cut(s) 219
BisI GCNGC 2 cut(s) 210, 342
BlsI GCNGC 2 cut(s) 211, 343
BmcAI AGTACT 2 cut(s) 235, 686
Bme1390I CCNGG 2 cut(s) 214, 299
BmgT120I GGNCC 2 cut(s) 421, 590
BmrFI CCNGG 2 cut(s) 214, 299
BmsI GCATC 1 cut(s) 338
BpmI CTGGAG 1 cut(s) 444
Bpu14I TTCGAA 1 cut(s) 677
BpuMI CCSGG 1 cut(s) 214
BsaJI CCNNGG 5 cut(s) 48, 147, 213, 297, 575
BsaXI ACNNNNNCTCC 2 cut(s) 442, 472
Bse1I ACTGG 2 cut(s) 19, 89
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 5 cut(s) 48, 147, 213, 297, 575
BseGI GGATG 1 cut(s) 254
BseMII CTCAG 3 cut(s) 102, 375, 651
BseNI ACTGG 2 cut(s) 19, 89
BseRI GAGGAG 1 cut(s) 595
BseXI GCAGC 2 cut(s) 221, 353
BshFI GGCC 2 cut(s) 423, 592
BsiHKAI GWGCWC 3 cut(s) 252, 574, 619
BsiSI CCGG 1 cut(s) 213
BslFI GGGAC 1 cut(s) 368
BsmAI GTCTC 1 cut(s) 112
BsmFI GGGAC 1 cut(s) 368
BsnI GGCC 2 cut(s) 423, 592
Bsp119I TTCGAA 1 cut(s) 677
Bsp1286I GDGCHC 3 cut(s) 252, 574, 619
Bsp143I GATC 4 cut(s) 219, 357, 453, 521
BspACI CCGC 1 cut(s) 445
BspANI GGCC 2 cut(s) 423, 592
BspCNI CTCAG 3 cut(s) 103, 374, 652
BspMAI CTGCAG 1 cut(s) 343
BspPI GGATC 1 cut(s) 529
BspT104I TTCGAA 1 cut(s) 677
BsrI ACTGG 2 cut(s) 19, 89
BssECI CCNNGG 5 cut(s) 48, 147, 213, 297, 575
BssMI GATC 4 cut(s) 219, 357, 453, 521
BssT1I CCWWGG 2 cut(s) 147, 575
Bst2UI CCWGG 1 cut(s) 299
Bst6I CTCTTC 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 677
BstDEI CTNAG 6 cut(s) 56, 111, 308, 361, 436, 660
BstEII GGTNACC 1 cut(s) 440
BstF5I GGATG 1 cut(s) 254
BstKTI GATC 4 cut(s) 222, 360, 456, 524
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 4 cut(s) 219, 357, 453, 521
BstNI CCWGG 1 cut(s) 299
BstPI GGTNACC 1 cut(s) 440
BstSCI CCNGG 2 cut(s) 212, 297
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 221, 353
BstX2I RGATCY 2 cut(s) 219, 521
BstYI RGATCY 2 cut(s) 219, 521
BsuRI GGCC 2 cut(s) 423, 592
BtsCI GGATG 1 cut(s) 254
BtsI GCAGTG 1 cut(s) 593
BtsIMutI CAGTG 2 cut(s) 82, 593
Cfr13I GGNCC 2 cut(s) 421, 590
Csp6I GTAC 2 cut(s) 234, 685
CviAII CATG 3 cut(s) 326, 403, 550
CviJI RGCY 9 cut(s) 129, 173, 212, 250, 344, 413, 423, 572, 592
CviKI_1 RGCY 9 cut(s) 129, 173, 212, 250, 344, 413, 423, 572, 592
CviQI GTAC 2 cut(s) 234, 685
DdeI CTNAG 6 cut(s) 56, 111, 308, 361, 436, 660
DpnI GATC 4 cut(s) 221, 359, 455, 523
DpnII GATC 4 cut(s) 219, 357, 453, 521
Eam1104I CTCTTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 580
Ecl136II GAGCTC 2 cut(s) 250, 572
Eco130I CCWWGG 2 cut(s) 147, 575
Eco24I GRGCYC 2 cut(s) 252, 574
Eco53kI GAGCTC 2 cut(s) 250, 572
Eco57I CTGAAG 1 cut(s) 651
Eco91I GGTNACC 1 cut(s) 440
EcoICRI GAGCTC 2 cut(s) 250, 572
EcoO65I GGTNACC 1 cut(s) 440
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 2 cut(s) 147, 575
EcoT22I ATGCAT 1 cut(s) 331
EcoT38I GRGCYC 2 cut(s) 252, 574
ErhI CCWWGG 2 cut(s) 147, 575
FaeI CATG 3 cut(s) 329, 406, 553
FaqI GGGAC 1 cut(s) 368
FatI CATG 3 cut(s) 325, 402, 549
Fnu4HI GCNGC 2 cut(s) 210, 342
FokI GGATG 1 cut(s) 241
FriOI GRGCYC 2 cut(s) 252, 574
Fsp4HI GCNGC 2 cut(s) 210, 342
FspBI CTAG 1 cut(s) 122
GluI GCNGC 2 cut(s) 210, 342
GsuI CTGGAG 1 cut(s) 444
HaeIII GGCC 2 cut(s) 423, 592
HapII CCGG 1 cut(s) 213
Hin1II CATG 3 cut(s) 329, 406, 553
HinfI GANTC 3 cut(s) 44, 432, 635
HpaII CCGG 1 cut(s) 213
HphI GGTGA 2 cut(s) 230, 434
Hpy166II GTNNAC 1 cut(s) 137
Hpy188I TCNGA 2 cut(s) 49, 535
Hpy188III TCNNGA 2 cut(s) 347, 461
Hpy8I GTNNAC 1 cut(s) 137
HpyAV CCTTC 1 cut(s) 512
HpyCH4V TGCA 4 cut(s) 209, 329, 341, 553
HpyF3I CTNAG 6 cut(s) 56, 111, 308, 361, 436, 660
Hsp92II CATG 3 cut(s) 329, 406, 553
Kzo9I GATC 4 cut(s) 219, 357, 453, 521
LmnI GCTCC 3 cut(s) 247, 569, 577
LpnPI CCDG 9 cut(s) 70, 226, 284, 311, 381, 474, 482, 612, 672
Lsp1109I GCAGC 2 cut(s) 221, 353
LweI GCATC 1 cut(s) 338
MaeI CTAG 1 cut(s) 122
MaeIII GTNAC 2 cut(s) 398, 440
MalI GATC 4 cut(s) 221, 359, 455, 523
MboI GATC 4 cut(s) 219, 357, 453, 521
MboII GAAGA 4 cut(s) 158, 516, 597, 644
MflI RGATCY 2 cut(s) 219, 521
MhlI GDGCHC 3 cut(s) 252, 574, 619
MnlI CCTC 7 cut(s) 34, 43, 60, 295, 412, 573, 581
Mph1103I ATGCAT 1 cut(s) 331
MseI TTAA 3 cut(s) 93, 102, 197
MspI CCGG 1 cut(s) 213
MspR9I CCNGG 2 cut(s) 214, 299
MvaI CCWGG 1 cut(s) 299
NciI CCSGG 1 cut(s) 214
NdeII GATC 4 cut(s) 219, 357, 453, 521
NlaIII CATG 3 cut(s) 329, 406, 553
NmuCI GTSAC 1 cut(s) 440
NsiI ATGCAT 1 cut(s) 331
NspV TTCGAA 1 cut(s) 677
PfeI GAWTC 3 cut(s) 44, 432, 635
PkrI GCNGC 2 cut(s) 211, 343
Psp124BI GAGCTC 2 cut(s) 252, 574
Psp6I CCWGG 1 cut(s) 297
PspEI GGTNACC 1 cut(s) 440
PspGI CCWGG 1 cut(s) 297
PspPI GGNCC 2 cut(s) 421, 590
PstI CTGCAG 1 cut(s) 343
PsuI RGATCY 2 cut(s) 219, 521
RsaI GTAC 2 cut(s) 235, 686
RsaNI GTAC 2 cut(s) 234, 685
SacI GAGCTC 2 cut(s) 252, 574
SaqAI TTAA 3 cut(s) 93, 102, 197
SatI GCNGC 2 cut(s) 210, 342
Sau3AI GATC 4 cut(s) 219, 357, 453, 521
Sau96I GGNCC 2 cut(s) 421, 590
ScaI AGTACT 2 cut(s) 235, 686
ScrFI CCNGG 2 cut(s) 214, 299
SduI GDGCHC 3 cut(s) 252, 574, 619
SetI ASST 9 cut(s) 127, 175, 243, 252, 346, 415, 442, 574, 613
SfaNI GCATC 1 cut(s) 338
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 677
SsiI CCGC 1 cut(s) 445
SspMI CTAG 1 cut(s) 122
SstI GAGCTC 2 cut(s) 252, 574
StyD4I CCNGG 2 cut(s) 212, 297
StyI CCWWGG 2 cut(s) 147, 575
TaqI TCGA 2 cut(s) 613, 677
TatI WGTACW 2 cut(s) 233, 684
TfiI GAWTC 3 cut(s) 44, 432, 635
Tru1I TTAA 3 cut(s) 93, 102, 197
Tru9I TTAA 3 cut(s) 93, 102, 197
TscAI CASTG 2 cut(s) 89, 600
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 2 cut(s) 209, 341
Tsp45I GTSAC 1 cut(s) 440
TspDTI ATGAA 1 cut(s) 660
TspRI CASTG 2 cut(s) 89, 600
XspI CTAG 1 cut(s) 122
ZrmI AGTACT 2 cut(s) 235, 686
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.