Rh2AG286000

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0015813
Physical Location & Seq
Forward (+)
34984648 .. 34991095
6448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG286000.1

Sequence Viewer

Length: 1488 bp
ATGGTACAGAGAGGCTATCACAGACCCAGACCACCATCTCCACACGCCATTAAACTCAACCTAATCCTCATTTTCGCCTTCTTCTTTTTGGTCTTCATCTTCTACACAAGGCAATGGCATCTGCTCGACGATCAGCAAAAGCAACAGCCTTTCTCCGATGAACATTCCCAAACTGACCGGATGCCAGATGCAGAACTTTGGGATGCTCCTCCTAGCTATGGTTTACGCTCATGTGTCAAGCCGACCTCTAGATATAAAGCTGCCACTGGATGGGACCACTACATGACTGTGAGGAGTAATGGAGGACTAAATCAGATGCGAACAGGCATTGCAGACATGGTGGCTGTGGCACGCATTATGAATGCGACCTTGGTCATTCCTCAATTGGATAAACGTTCATTCTGGCATGACTCGAGTACATTTTCAGATATATTTGATGAACTTCACTTCATTGAAACTCTGCAAGAAGATGTCAAGATTGTCAAGGAGCTCCCACAGGAATTGGCAAATGTTCCTCGGGCGAGGAAGCATTTCACATCCTGGGCTAGCACCAGTTACTATGAAGACATGACACAGCTATGGAAAGAATATCAGGTGATTCATGTTGCAAAATCAGATTCTCGACTTGCAAACAATGATCTCCCTCTTGATATCCAGAGGTTGAGATGCCGTGCCTTGTATCATGCGCTCCGTTTCTCTCCCCCAATTGAGAGCTTTGGAAAGAAGCTGGTGGAGCGGCTTAGAGCACGTGGGGGAAAATATATTGCTCTTCATTTGAGATATGAGAAAGACATGTTGTCTTTTACTGGTTGTTCTTATGGTTTGACTGATGAGGAATCCGAGGAACTAAGAATAATGAGGGAGAACACAAATCACTGGAAAGTTAAAAAGATTAACTCTACTGAGCAGAGACTAGGTGGCTTCTGTCCACTAACTCCCAAGGAGATTGGCATATTTCTTCGAGCTCTCGGTTATCCACCATCATCGTTAATATATATTGCAGCTGGGGAGATCTATGGTGGTAGTACTCACCTTTTGGAGCTCACATCCCGTTTTCCAAATATAGTTTTCAAGGAAACACTTGCGACCCAGGAGGAACTAAGCGCATTTACTAATCATGCATCTCAAACTGCAGCTCTTGATTATGTGATCTCAGTTGAAAGTGATGTGTTTGTCCCATCATATTCTGGTAACATGGCAAGAGCTGTTGAGGGCCAACGCAGATTCTTAGGTCACCAAAAAACAATCACTCCAGATAGGAAAGGACTTGTTGAAGTTTTTGATATGCTGGAAAATGGACAACAAACAGAAGGATCTTCGCTGTCACATATTATACAGACCATGCACAAAAATAGGAAAGGAGCTCCAAGGAGGAGAAGAGGGCCACTGCCAGAAATGAAAGGTCGAGCACGATTGAGAACTGAAGAATCTTTCTATGAAAACCCTTACCCTGAGTGTATATGTGCTTCGAAAAAGTACTTGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

495

Amino Acids

56.98

Weight (kDa)

8.98

Isoelectric Point (pI)

53.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 94 - 409 5.3e-105 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64600
fragaria_vesca FvH4_1g24200 FvH4_1g24200 FvH4_1g24200
rosa_chinensis RchiOBHm_Chr2g0120631 RchiOBHm_Chr2g0120641
rosa_laevigata RLG00000018563
rosa_multiflora Rmu_sc0001844.1_g000005
rosa_roxburghii Rroxscaffold_2G00123270
rosa_rugosa Rorug02G0230000
rosa_samantha Rh2AG286000 Rh2BG297800 Rh2CG275500 Rh2DG311500
rosa_wichuraiana Rw2G022970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 736
AciI CCGC 1 cut(s) 736
AclI AACGTT 1 cut(s) 394
AclWI GGATC 1 cut(s) 1321
AcuI CTGAAG 1 cut(s) 1443
AcvI CACGTG 1 cut(s) 749
AfaI GTAC 4 cut(s) 6, 418, 1027, 1478
AfiI CCNNNNNNNGG 2 cut(s) 218, 270
AflIII ACRYGT 1 cut(s) 792
AgsI TTSAA 4 cut(s) 455, 1072, 1160, 1274
AhdI GACNNNNNGTC 1 cut(s) 796
AjnI CCWGG 2 cut(s) 539, 1089
AjuI GAANNNNNNNTTGG 3 cut(s) 353, 385, 1463
Alw21I GWGCWC 6 cut(s) 492, 748, 967, 1044, 1366, 1411
Alw26I GTCTC 1 cut(s) 904
AlwI GGATC 1 cut(s) 1321
Ama87I CYCGRG 2 cut(s) 412, 516
AoxI GGCC 2 cut(s) 1213, 1382
ApeKI GCWGC 3 cut(s) 260, 1001, 1133
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 2 cut(s) 688, 1106
AspS9I GGNCC 3 cut(s) 274, 1213, 1382
AsuHPI GGTGA 3 cut(s) 607, 1022, 1226
AsuII TTCGAA 1 cut(s) 1469
AsuNHI GCTAGC 1 cut(s) 545
AvaI CYCGRG 2 cut(s) 412, 516
AvaII GGWCC 1 cut(s) 274
BanII GRGCYC 4 cut(s) 492, 967, 1044, 1366
BarI GAAGNNNNNNTAC 2 cut(s) 1450, 1482
BbrPI CACGTG 1 cut(s) 749
BbsI GAAGAC 2 cut(s) 85, 570
Bbv12I GWGCWC 6 cut(s) 492, 748, 967, 1044, 1366, 1411
BbvI GCAGC 3 cut(s) 247, 1013, 1145
BccI CCATC 4 cut(s) 43, 264, 988, 1186
BceAI ACGGC 1 cut(s) 654
BciT130I CCWGG 2 cut(s) 541, 1091
BcoDI GTCTC 1 cut(s) 904
BfaI CTAG 4 cut(s) 213, 249, 546, 914
BfmI CTRYAG 1 cut(s) 1131
BglII AGATCT 1 cut(s) 1011
BisI GCNGC 4 cut(s) 261, 737, 1002, 1134
BlsI GCNGC 4 cut(s) 262, 738, 1003, 1135
BmcAI AGTACT 2 cut(s) 1027, 1478
Bme1390I CCNGG 2 cut(s) 541, 1091
Bme18I GGWCC 1 cut(s) 274
BmeRI GACNNNNNGTC 1 cut(s) 796
BmeT110I CYCGRG 2 cut(s) 412, 516
BmgT120I GGNCC 3 cut(s) 274, 1213, 1382
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 2 cut(s) 541, 1091
BmsI GCATC 7 cut(s) 127, 171, 178, 193, 306, 656, 1130
BmtI GCTAGC 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 371
BpiI GAAGAC 2 cut(s) 85, 570
BpmI CTGGAG 1 cut(s) 1236
Bpu14I TTCGAA 1 cut(s) 1469
BsaAI YACGTR 1 cut(s) 749
BsaJI CCNNGG 7 cut(s) 369, 515, 540, 840, 939, 1089, 1367
BsaWI WCCGGW 1 cut(s) 177
BsaXI ACNNNNNCTCC 2 cut(s) 1234, 1264
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 270
Bse1I ACTGG 4 cut(s) 271, 552, 811, 881
Bse3DI GCAATG 2 cut(s) 119, 327
BseBI CCWGG 2 cut(s) 541, 1091
BseDI CCNNGG 7 cut(s) 369, 515, 540, 840, 939, 1089, 1367
BseGI GGATG 5 cut(s) 186, 208, 275, 536, 1046
BseLI CCNNNNNNNGG 2 cut(s) 218, 270
BseMI GCAATG 2 cut(s) 119, 327
BseMII CTCAG 3 cut(s) 894, 1167, 1443
BseNI ACTGG 4 cut(s) 271, 552, 811, 881
BseRI GAGGAG 3 cut(s) 198, 307, 1387
BseXI GCAGC 3 cut(s) 247, 1013, 1145
BseYI CCCAGC 1 cut(s) 1004
BshFI GGCC 2 cut(s) 1215, 1384
BsiHKAI GWGCWC 6 cut(s) 492, 748, 967, 1044, 1366, 1411
BsiHKCI CYCGRG 2 cut(s) 412, 516
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 2 cut(s) 287, 1160
BslI CCNNNNNNNGG 2 cut(s) 218, 270
BsmAI GTCTC 1 cut(s) 904
BsmFI GGGAC 2 cut(s) 287, 1160
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 2 cut(s) 1215, 1384
BsoBI CYCGRG 2 cut(s) 412, 516
Bsp119I TTCGAA 1 cut(s) 1469
Bsp1286I GDGCHC 6 cut(s) 492, 748, 967, 1044, 1366, 1411
Bsp143I GATC 5 cut(s) 130, 637, 1011, 1149, 1313
BspACI CCGC 1 cut(s) 736
BspANI GGCC 2 cut(s) 1215, 1384
BspCNI CTCAG 3 cut(s) 895, 1166, 1444
BspLI GGNNCC 1 cut(s) 275
BspMAI CTGCAG 1 cut(s) 1135
BspOI GCTAGC 1 cut(s) 549
BspPI GGATC 1 cut(s) 1321
BspQI GCTCTTC 1 cut(s) 774
BspT104I TTCGAA 1 cut(s) 1469
BsrBI CCGCTC 1 cut(s) 736
BsrDI GCAATG 2 cut(s) 119, 327
BsrI ACTGG 4 cut(s) 271, 552, 811, 881
BssECI CCNNGG 7 cut(s) 369, 515, 540, 840, 939, 1089, 1367
BssMI GATC 5 cut(s) 130, 637, 1011, 1149, 1313
BssT1I CCWWGG 3 cut(s) 369, 939, 1367
Bst2UI CCWGG 2 cut(s) 541, 1091
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 2 cut(s) 774, 1372
BstBAI YACGTR 1 cut(s) 749
BstBI TTCGAA 1 cut(s) 1469
BstC8I GCNNGC 2 cut(s) 352, 547
BstDEI CTNAG 7 cut(s) 740, 848, 903, 1100, 1153, 1228, 1452
BstEII GGTNACC 1 cut(s) 1232
BstF5I GGATG 5 cut(s) 186, 208, 275, 536, 1046
BstHHI GCGC 2 cut(s) 688, 1106
BstKTI GATC 5 cut(s) 133, 640, 1014, 1152, 1316
BstMAI GTCTC 1 cut(s) 904
BstMBI GATC 5 cut(s) 130, 637, 1011, 1149, 1313
BstMWI GCNNNNNNNGC 1 cut(s) 733
BstNI CCWGG 2 cut(s) 541, 1091
BstNSI RCATGY 1 cut(s) 796
BstPAI GACNNNNGTC 1 cut(s) 371
BstPI GGTNACC 1 cut(s) 1232
BstSCI CCNGG 2 cut(s) 539, 1089
BstSFI CTRYAG 1 cut(s) 1131
BstV1I GCAGC 3 cut(s) 247, 1013, 1145
BstV2I GAAGAC 2 cut(s) 85, 570
BstX2I RGATCY 2 cut(s) 1011, 1313
BstYI RGATCY 2 cut(s) 1011, 1313
BsuRI GGCC 2 cut(s) 1215, 1384
BtsCI GGATG 5 cut(s) 186, 208, 275, 536, 1046
BtsI GCAGTG 1 cut(s) 1385
BtsIMutI CAGTG 3 cut(s) 264, 874, 1385
Cac8I GCNNGC 2 cut(s) 352, 547
CfoI GCGC 2 cut(s) 688, 1106
Cfr13I GGNCC 3 cut(s) 274, 1213, 1382
Csp6I GTAC 4 cut(s) 5, 417, 1026, 1477
CspCI CAANNNNNGTGG 2 cut(s) 483, 518
CviQI GTAC 4 cut(s) 5, 417, 1026, 1477
DdeI CTNAG 7 cut(s) 740, 848, 903, 1100, 1153, 1228, 1452
DpnI GATC 5 cut(s) 132, 639, 1013, 1151, 1315
DpnII GATC 5 cut(s) 130, 637, 1011, 1149, 1313
DriI GACNNNNNGTC 1 cut(s) 796
Eam1104I CTCTTC 2 cut(s) 774, 1372
Eam1105I GACNNNNNGTC 1 cut(s) 796
EarI CTCTTC 2 cut(s) 774, 1372
Ecl136II GAGCTC 4 cut(s) 490, 965, 1042, 1364
Eco130I CCWWGG 3 cut(s) 369, 939, 1367
Eco24I GRGCYC 4 cut(s) 492, 967, 1044, 1366
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 4 cut(s) 490, 965, 1042, 1364
Eco57I CTGAAG 1 cut(s) 1443
Eco72I CACGTG 1 cut(s) 749
Eco88I CYCGRG 2 cut(s) 412, 516
Eco91I GGTNACC 1 cut(s) 1232
EcoICRI GAGCTC 4 cut(s) 490, 965, 1042, 1364
EcoO65I GGTNACC 1 cut(s) 1232
EcoRII CCWGG 2 cut(s) 539, 1089
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 3 cut(s) 369, 939, 1367
EcoT22I ATGCAT 1 cut(s) 1123
EcoT38I GRGCYC 4 cut(s) 492, 967, 1044, 1366
ErhI CCWWGG 3 cut(s) 369, 939, 1367
FaqI GGGAC 2 cut(s) 287, 1160
Fnu4HI GCNGC 4 cut(s) 261, 737, 1002, 1134
FokI GGATG 5 cut(s) 193, 215, 282, 523, 1033
FriOI GRGCYC 4 cut(s) 492, 967, 1044, 1366
Fsp4HI GCNGC 4 cut(s) 261, 737, 1002, 1134
FspBI CTAG 4 cut(s) 213, 249, 546, 914
GlaI GCGC 2 cut(s) 687, 1105
GluI GCNGC 4 cut(s) 261, 737, 1002, 1134
GsaI CCCAGC 1 cut(s) 1008
GsuI CTGGAG 1 cut(s) 1236
HaeIII GGCC 2 cut(s) 1215, 1384
HapII CCGG 1 cut(s) 178
HhaI GCGC 2 cut(s) 688, 1106
Hin6I GCGC 2 cut(s) 686, 1104
HinP1I GCGC 2 cut(s) 686, 1104
HinfI GANTC 6 cut(s) 410, 598, 617, 836, 1224, 1427
HpaII CCGG 1 cut(s) 178
HphI GGTGA 3 cut(s) 607, 1022, 1226
Hpy166II GTNNAC 2 cut(s) 224, 929
Hpy188I TCNGA 5 cut(s) 157, 315, 427, 616, 841
Hpy188III TCNNGA 7 cut(s) 249, 475, 621, 647, 655, 1139, 1253
Hpy8I GTNNAC 2 cut(s) 224, 929
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 2 cut(s) 88, 1304
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 394, 748
HpyCH4V TGCA 9 cut(s) 191, 332, 463, 608, 629, 1001, 1121, 1133, 1345
HpyF10VI GCNNNNNNNGC 1 cut(s) 733
HpyF3I CTNAG 7 cut(s) 740, 848, 903, 1100, 1153, 1228, 1452
HpySE526I ACGT 2 cut(s) 394, 748
HspAI GCGC 2 cut(s) 686, 1104
Kzo9I GATC 5 cut(s) 130, 637, 1011, 1149, 1313
LguI GCTCTTC 1 cut(s) 774
LmnI GCTCC 8 cut(s) 211, 487, 495, 693, 733, 1039, 1361, 1369
Lsp1109I GCAGC 3 cut(s) 247, 1013, 1145
LweI GCATC 7 cut(s) 127, 171, 178, 193, 306, 656, 1130
MaeI CTAG 4 cut(s) 213, 249, 546, 914
MaeII ACGT 2 cut(s) 394, 748
MaeIII GTNAC 4 cut(s) 554, 1190, 1232, 1323
MalI GATC 5 cut(s) 132, 639, 1013, 1151, 1315
MbiI CCGCTC 1 cut(s) 736
MboI GATC 5 cut(s) 130, 637, 1011, 1149, 1313
MfeI CAATTG 2 cut(s) 383, 705
MflI RGATCY 2 cut(s) 1011, 1313
MhlI GDGCHC 6 cut(s) 492, 748, 967, 1044, 1366, 1411
MluCI AATT 3 cut(s) 383, 500, 705
MlyI GAGTC 1 cut(s) 404
Mph1103I ATGCAT 1 cut(s) 1123
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 4 cut(s) 51, 885, 894, 989
MslI CAYNNNNRTG 2 cut(s) 287, 577
MspA1I CMGCKG 1 cut(s) 1004
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 2 cut(s) 541, 1091
MunI CAATTG 2 cut(s) 383, 705
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 2 cut(s) 541, 1091
MwoI GCNNNNNNNGC 1 cut(s) 733
NdeII GATC 5 cut(s) 130, 637, 1011, 1149, 1313
NheI GCTAGC 1 cut(s) 545
NlaIV GGNNCC 1 cut(s) 275
NmuCI GTSAC 2 cut(s) 1232, 1323
NsiI ATGCAT 1 cut(s) 1123
NspI RCATGY 1 cut(s) 796
NspV TTCGAA 1 cut(s) 1469
PaeR7I CTCGAG 1 cut(s) 412
PciI ACATGT 1 cut(s) 792
PciSI GCTCTTC 1 cut(s) 774
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 5 cut(s) 598, 617, 836, 1224, 1427
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 4 cut(s) 262, 738, 1003, 1135
PleI GAGTC 1 cut(s) 404
PmaCI CACGTG 1 cut(s) 749
PmlI CACGTG 1 cut(s) 749
PpsI GAGTC 1 cut(s) 404
Ppu21I YACGTR 1 cut(s) 749
PscI ACATGT 1 cut(s) 792
PshAI GACNNNNGTC 1 cut(s) 371
Psp124BI GAGCTC 4 cut(s) 492, 967, 1044, 1366
Psp1406I AACGTT 1 cut(s) 394
Psp6I CCWGG 2 cut(s) 539, 1089
PspCI CACGTG 1 cut(s) 749
PspEI GGTNACC 1 cut(s) 1232
PspFI CCCAGC 1 cut(s) 1004
PspGI CCWGG 2 cut(s) 539, 1089
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 3 cut(s) 274, 1213, 1382
PspXI VCTCGAGB 1 cut(s) 412
PstI CTGCAG 1 cut(s) 1135
PsuI RGATCY 2 cut(s) 1011, 1313
PvuII CAGCTG 1 cut(s) 1004
RsaI GTAC 4 cut(s) 6, 418, 1027, 1478
RsaNI GTAC 4 cut(s) 5, 417, 1026, 1477
RseI CAYNNNNRTG 2 cut(s) 287, 577
SacI GAGCTC 4 cut(s) 492, 967, 1044, 1366
SapI GCTCTTC 1 cut(s) 774
SaqAI TTAA 4 cut(s) 51, 885, 894, 989
SatI GCNGC 4 cut(s) 261, 737, 1002, 1134
Sau3AI GATC 5 cut(s) 130, 637, 1011, 1149, 1313
Sau96I GGNCC 3 cut(s) 274, 1213, 1382
ScaI AGTACT 2 cut(s) 1027, 1478
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 2 cut(s) 541, 1091
SduI GDGCHC 6 cut(s) 492, 748, 967, 1044, 1366, 1411
SfaNI GCATC 7 cut(s) 127, 171, 178, 193, 306, 656, 1130
SfcI CTRYAG 1 cut(s) 1131
Sfr274I CTCGAG 1 cut(s) 412
SfuI TTCGAA 1 cut(s) 1469
SinI GGWCC 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 412
SmiMI CAYNNNNRTG 2 cut(s) 287, 577
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
Sse9I AATT 3 cut(s) 383, 500, 705
SsiI CCGC 1 cut(s) 736
SspMI CTAG 4 cut(s) 213, 249, 546, 914
SstI GAGCTC 4 cut(s) 492, 967, 1044, 1366
StyD4I CCNGG 2 cut(s) 539, 1089
StyI CCWWGG 3 cut(s) 369, 939, 1367
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 2 cut(s) 397, 751
TaqI TCGA 6 cut(s) 126, 413, 622, 961, 1405, 1469
TasI AATT 3 cut(s) 383, 500, 705
TatI WGTACW 3 cut(s) 416, 1025, 1476
TauI GCSGC 1 cut(s) 739
TfiI GAWTC 5 cut(s) 598, 617, 836, 1224, 1427
Tru1I TTAA 4 cut(s) 51, 885, 894, 989
Tru9I TTAA 4 cut(s) 51, 885, 894, 989
TscAI CASTG 3 cut(s) 271, 881, 1392
TseFI GTSAC 2 cut(s) 1232, 1323
TseI GCWGC 3 cut(s) 260, 1001, 1133
Tsp45I GTSAC 2 cut(s) 1232, 1323
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 3 cut(s) 271, 881, 1392
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 274
XbaI TCTAGA 1 cut(s) 248
XceI RCATGY 1 cut(s) 796
XhoI CTCGAG 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 4 cut(s) 213, 249, 546, 914
ZrmI AGTACT 2 cut(s) 1027, 1478
Zsp2I ATGCAT 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.